β1 signaling in in vitro endothelial cell models
Báo cáo khoa học: Therapeutic targeting of molecules involved in leukocyte–endothelial cell interactions potx
Ngày tải lên :
07/03/2014, 12:20
... redistribution from intracellular pools [20] Integrins mediate cell cell, cell extracellular matrix and cell pathogen interactions by binding to distinct, but overlapping, FEBS Journal 273 (2006) ... proteincoupled receptor (GPCR) signaling [6] Several antiinflammatory drugs indirectly inhibit components involved in leukocyte endothelial cell interactions For example, compounds that block interleukin ... containing sulfated sialyl-Lewis X like molecules Inhibiting leukocyte rolling by blocking selectins affects the accumulation of leukocytes in many experimental settings [14,15] Blocking selectin...
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Báo cáo y học: "Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblasts" potx
Ngày tải lên :
09/08/2014, 08:23
... H, Onishi Y, Inukai K, Anai M, Fukushima Y, et al.: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain J Biol Chem ... plated at 10,000 cells/cm2 in 96-well plates and incubated overnight in Ham F12/DMEM media containing 10% FBS Cells were then serumstarved for 24 hours in the same media containing 0.5% BSA then ... Syp/IRS-1 also demonstrated an increased interaction in the basal state between IRS-1 and Syp in OA Obs, unlike in normal cells Since there is an increase in IGF-1 production in OA Obs with a concomitant...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
Ngày tải lên :
12/08/2014, 23:21
... x104 cells/well in 96-well plates containing various concentrations of test compound The infected cells were incubated for days at 37°C in 5% CO2 Antiviral activity was measured by determining ... (PD) of didanosine (ddI) alone and in combination with azidothymidine (AZT) in human T-cells; a stochastic biochemical approach to antiretroviral nucleoside drug combination in inhibiting HIV-reverse ... combination is shown in Fig 4A In this experiment, TFV- Table 1: Evaluation of drug combinations for inhibition of HIV-1 in MT-2 cell culture Combinations Analysis Method Median-Effecta (Combination...
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Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes" pdf
Ngày tải lên :
13/08/2014, 09:20
... lowering rates of transmission, given the relative inefficiency of infection [35,36] The effect of CQ on DC-SIGN binding in vivo remains to be determined, but if the DC-SIGN binding is indeed ... important for 2G12 binding [23], as well as an additional site in the V3 region of gp120 (data not shown) The PNG site expressing carbohydrates involved in 2G12 binding (397) is lost in the V4 region ... 1B), suggesting a co-receptor phenotype restriction to inhibition by CQ The observed inhibition by CQ was not due to enhanced cell death since cell In order to determine whether the inhibitory...
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Characterization of sema5a plexin b3 signaling in the oligodendrocyte cell line OLN 93
Ngày tải lên :
03/10/2015, 20:32
... intracellular domain containing proline-rich motifs Class semaphorins have PDZ-binding domain at their C-termini of intracellular domain The specific receptors for semaphorins are plexins The plexins are ... Furthermore, the cytoplasmic domain of plexin-B family has a specific sequence responsible for binding PDZ domain-containing protein (PDZ-binding domain) at the C-terminus Plexin-B1 interacts directly with ... are required from origins in neural tube to final myelination stage, and and regulated by both cell- extrinsic factors and cell- intrinsic factors Cell- intrinsic factors include olig-1, olig-2...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based ... UK); Cellomics NF-jB Hit kit Evaluation Screening (Cellomics Inc., Pittsburg, PA, USA); DMEM (Gibco, Invitrogen); 200 mm l-glutamine; fetal bovine serum (Gibco Invitrogen, Virkon, Pzer internal ... lL; prewarmed in a water bath at 37 C) was dispensed into each well and incubated for 15 (prewarming xative is critical to maintaining cell integrity) After 15 min, the plates were inverted to...
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Báo cáo y học: "Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells." pot
Ngày tải lên :
11/08/2014, 03:20
... inhibitor), Wortmannin (PI3K inhibitor), SB203580 (p38 MAPK inhibitor) and SP600125 (c-jun N-terminal Kinase [JNK] inhibitor) to determine which kinase pathways were being activated by insulin ... We used kinase inhibitors to determine their influences on insulin and TNFα stimulated nuclear import of NFκB in endothelial cells While MEK1/2, p38 and JNK inhibitors reduced insulin and TNFα-stimulated ... positive controls (insulin or TNFα with no inhibitor) #, P < 0.05 vs insulin plus TNFα (no inhibitors) (SFM) serum-free medium; (INHB) inhibitors; (NI) no inhibitors; (INS) insulin; (TNFα) Tumor...
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![Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc](https://media.store123doc.com/images/document/2014_08/12/medium_9S3XH52qK4.jpg)
Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
Ngày tải lên :
11/08/2014, 08:21
... quickly, inhibiting PMNs adhesion to HUVEC within 10–20 min, The author(s) declare that they have no competing interests PGE2: prostaglandin E2; PGI2: prostacyclin I2; HUVEC: Human Umbilical Vein Endothelial ... heat-inactivated bovine calf serum (BCS), 100 U/ml penicillin, 100 µg/ml streptomycin, U.I./ml heparin, 12 µg/ml bovine brain extract and 200 mM glutamine HUVEC were grown to confluence in flasks ... of CAMs in modulating PMN adhesion to ECs is well known The CAMs involved in leukocyte trafficking constitute excellent targets for pharmacological modulation of the cellular response in inflammation...
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Báo cáo y học: " Reduced CD4 T cell activation and in vitro susceptibility to HIV-1 infection in exposed uninfected Central Africans" pptx
Ngày tải lên :
13/08/2014, 09:20
... mononuclear cells to in vitro HIV-1 infection Susceptibility of peripheral blood mononuclear cells to in vitro HIV-1 infection PBMCs (105 cells/well in 96-well microplates) from 45 EUs and 44 UCs were infected ... shown to influence in vitro infectability with R5 HIV-1 [37-41] and R5 variants are largely prevalent in infected individuals in Bangui [42] Therefore, low levels of CCR5-expressing CD4 T cells ... PBMC" Viral inoculums able to consistently infect standard PBMCs were determined in preliminary assays For each individual, PBMCs (105 cells/ well in 96-well microplates) were infected in quadruplicate...
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Báo cáo y học: " Liver sinusoidal endothelial cell modulation upon resection and shear stress in vitro" doc
Ngày tải lên :
13/08/2014, 13:20
... factor influencing lining endothelial cells Consistent with the increased permeability in zone and zone following PHx, accumulation of lipid droplets was evident 10 hrs post surgery, persisting ... of endothelial cells or between tumor cells and endothelial cells Activation of VEGFR-2 has been shown to be involved in the formation of complexes with various cytoplasmic proteins including ... elevated number of endothelial cell gaps in both periportal and pericentral areas Shear stress in vitro induces in endothelial cells membrane translocation of VEGFR-2 and neuropilin-1 It is conceivable...
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Báo cáo y học: "The association of endothelial cell signaling, severity of illness, and organ dysfunction in sepsis" ppt
Ngày tải lên :
13/08/2014, 21:21
... of 12 Figure Endothelial cell response in sepsis (a) Leukocyte trafficking Activated endothelial cells (red-colored cells) express increased levels of E-selectin, P-selectin, intercellular adhesion ... suspected infection Suspected infection was defined as a clinical suspicion of an infectious etiology as assessed by the treating clinician, and Page of 12 determined by interviewing the treating physician ... of endothelial cell activation in the response to infection, we correlated the selected biomarkers which individually represent various components of the endothelial cell signaling pathway Using...
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The role of caspase 1 in a murine model of influenza pneumonitis, and studies on cell death inhibitors in vitro
Ngày tải lên :
13/10/2015, 16:41
... embryonic stem cells FBS foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected ... caspase inhibitors and a p53 inhibitor in vitro 55 CHAPTER 56 vi RESULTS 57 3.1 INFLUENZA A INFECTION OF RAW264.7 MURINE MACROPHAGE CELL- LINE IN VITRO 57 ... 54 2.29.1 Influenza A infection of RAW264.7 murine macrophage cell line in vitro 54 2.29.2 An investigation of the role of caspase-1 in an in vivo mouse model of influenza pneumonitis...
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Nghiên cứu kỹ thuật bào chế, đánh giá khả năng kháng khuẩn trên in vitro của chế phẩm kem Silver sulfa 1%
Ngày tải lên :
29/10/2012, 11:45
... quinon, alcaloid, tinh dầu, acid hữu cơ, phytosterol - Dịch chiết cồn: quinon, flavonoid, alcaloid, acid hữu cơ, sterol, đường khử, tanin, anthocynosid - Dịch chiết nước acid: alcaloid, tanin, ... Gelatin - - - Acid hữu -Tinh thể Na CO -Xanh Bromothymol + + + + + + Đường khử -Fehling A- B + + - Anthocyanosid - HCl; KOH - - - Tinh dầu -Bay - - - 10 Caroten - H SO 10% - - - 11 Saponin + ... 12h, lọc, sấy Tinh thể P lần Tinh thể P lần Hình 3.4: Sơ đồ quy trình chiết xuất, tinh chế chất P2 sau sắc ký lớp chế hoá 3.4 Nhận dạng chất P2 chiết - Thể chất: tinh thể P2 tinh thể hình kim,...
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Nghiên cứu kỹ thuật bào chế, đánh giá khả năng kháng khuẩn trên in Vitro của chế phẩm kem Silver Sulfa 1%
Ngày tải lên :
01/04/2013, 15:10
... CT3 Silvirin 1% tương đương Kết đo kháng khuẩn invitro Bảng 9:So sánh khả khuẩn invitrô CT3 Silvirin 1% Vi khuẩn S.aureus P.aeruginosa E.coli Đường kính vùng ức chế (mm) CT3 Silvirin 1% 10 10 ... Triglycorides Glycrol Chất bảo quản Nước tinh khiết vđ CT 3: Sulfadiazin bạc Alcol cetostearic Glycerin monostearat Vaselin Paraffin Tween 60 Chất bảo quản Nước tinh khiết vđ 1,0g 1,4g 0,6g 6,5g 11,0g ... thương binh lên tới 70% - 85% Thuốc mỡ sát khuẩn điều trị vết bỏng chỗ * Kem maduxin;* Mỡ cao vàng;* Damcream * Chế phẩm chứa hoạt chất sulfadiazin bạc : Sivadene (Mỹ), Flammazin (Pháp), Silvirin...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
Ngày tải lên :
18/02/2014, 16:20
... Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling and proteolysis inhibition, indicating ... the integrin system as one major sensor of hepatocyte swelling [12–14] Integrin-inhibitory peptides exhibiting an arginine-glycine-aspartic acid (RGD) motif abolish hypoosmotic osmosignaling...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
Ngày tải lên :
07/03/2014, 11:20
... GSM-900 and cell stress in skin models S Sanchez et al expression under stress conditions has been reported in a number of cell types, including skin cells The major HSPs expressed in the skin [5] ... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding ... expression (Fig 6J) In the case of Hsp70 (Fig 6G–I), instead of being expressed only in the cytoplasm as in NHDFe, it was also expressed in the nucleus UVB exposure induced a slight increase in Hsp70 expression,...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... CDO/chemotherapy combinations in GEO cells resulted in significant reductions in cell number This was further investigated by assessing BrdU incorporation Results confirmed the synergistic reduction in cell number, ... increased in GEO but unchanged in HCT116 cells (B) Standard immunoblots for cyclin and CDK levels in GEO cells (C) Immunoprecipitation experiments highlighting cyclin/CDK interactions in the GEO cell ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy...
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Báo cáo khoa học: Pathways involved in testicular germ cell apoptosis induced by H2O2 in vitro doc
Ngày tải lên :
16/03/2014, 04:20
... apoptosis in testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction ... pathways involved in testicular germ cell apoptosis pertaining to specific treatments As previously shown [7], testicular cell apoptosis follows both extrinsic and intrinsic pathways The extrinsic ... resuspended in medium-199 containing 100 lgÆmL)1 streptomycin sulfate and 100 IUÆmL)1 penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess...
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Báo cáo khoa học: Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle pdf
Ngày tải lên :
16/03/2014, 05:20
... Regeard et al In vitro HCV infection models mutations Furthermore, it should be kept in mind that, in this system, hundreds of RNA copies per cell are present, in contrast to 5–50 copies in infected ... lymphocyte cell lines and also for dendritic cells, independently of CD81 expression [51,52] The limited involvement of CD81 in the binding process was further illustrated by the poor binding inhibition ... hepatocytes infected with patient sera are the most physiological in vitro model at present, the difficulty of obtaining cells and intrinsic technical constraints make it hard to use in everyday...
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