β lapachone treatment induces the death of erythrocytes and endocardium
Báo cáo y học: " Functional cooperation of of IL-1b and RGS4 in the brachial plexus avulsion mediated brain reorganizatio" pps
... they have been implicated in the excessive production and processing of b-amyloid precursor protein and the plaque-associated proteins [28]; They can induce the production of various growth and ... the addition of ml of 90% sucrose prepared in MBS at pH 6.8 and placed at the bottom of an ultracentrifuge tube The lysate was then overlaid with ml of 35% sucrose and ml of 5% sucrose, both ... 2, RGS4 and PKACb mRNA expression in cerebral cortex was 1.02 ± 0.17 and 0.97 ± 0.21, 2.77 ± 0.34 and 2.97 ± 0.48, 2.59 ± Page of 11 0.39 and 3.12 ± 1.13 folds of control in 1, and month of operation...
- 11
- 299
- 0
Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... (slopes of –3.37 and –3.48, respectively) However, the efficiency using the PBMC template was considerably lower with a slope of –3.90 The magnitude of the difference in the C(t) between PBMC and the ... aliquot of PBMC was assayed for GAPDH on each day and either the raw C(t) was recorded or the amount was correlated to the standard curve generated on the same day The CV was then calculated for the ... standards Data analysis Results are expressed as the mean ± standard error of the CE or the RE, unless otherwise indicated The withintissue coefficient of variation (CV) was calculated as the...
- 9
- 557
- 0
Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
... ces 003 rof C 27 ta noisnetxe lanif dna ces 09 rof C 27 pmet noisnetxe ,ces 54 rof C 06 pmet gnilaenna ,ces 54 rof C 49 ta noitarutaned ,ces 06 rof C 49 ta noitarutaned laitini ,sgniwollof eht ... rof nitca -β dna 1H2PYC ,A3PYC fo tcudorp RCP lm siserohportcele leg esoraga %1 no nur erew selpmas eht ,nitca -β dna 1H2PYC ,A3PYC fo noisserpxe eneg eht yfitnauq oT suoiverp sa emas si RCP rof ... saw eneg 1H2PYC dna A3PYC rof oitar noisserpxe evitaleR denimreted saw )adanaC ,satnemreF( reluR eneG reddal AND bK dradnatS ot ecnerefer htiw nitca -β dna 1H2PYC ,A3PYC rof ytitnauq fo noitatitnauq...
- 7
- 291
- 0
Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of the ... monitoring the effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The ... Amplification of the target DNA, and hybridization to a fluorogenic probe are conducted in a single PCR and therefore the chances of possible contamination are minimised The main advantage of real-time...
- 10
- 458
- 0
báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of the ... monitoring the effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The ... Amplification of the target DNA, and hybridization to a fluorogenic probe are conducted in a single PCR and therefore the chances of possible contamination are minimised The main advantage of real-time...
- 10
- 440
- 0
Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot
... region of known HPVs and should allow for detection of any of these types The second qPCR assay targets the coding region of the hypervariable loop V of the L1 gene of HPV6, 11, 16 and 18 These ... types of HPV represented in an FDA approved vaccine, types 6,11,16, and 18 Page 16 of 17 The ability of the assay to distinguish between these types of HPV in a complex mixture of DNA and in the ... contained a mixture of × 105 copies of HPV6 L1, × 104 copies of HPV11 L1, × 103 copies of HPV16 L1 and × 102 copies of HPV18 L1 The assay was performed in the presence or absence of 100 ng of DG-75 DNA...
- 17
- 413
- 0
Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
... infiltration of muscular and connective tissues of the retroorbital space is a histological hallmark of Graves’ ophthalmopathy The pathogenesis of Graves’ ophthalmopathy and whether it is the result of ... the inhibition of one dye caused by another without emission of a proton The reporter dye is found on the 5’ end of the probe and the quencher at the 3’ end Figure 18 TaqMan® probe The red circle ... away from the quencher and to emit its energy, which is then quantified by the instrument (Figure 19) The greater the number of cycles of PCR takes place, the higher the incidence of Taqman®...
- 98
- 145
- 0
Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
... throughout the year, and the abundance ratio of the microcystin-degrading bacteria increased to approximately 0.005% of the total bacteria The number of cells increased in fall, and the number of microcystin-degrading ... in the biofilm of the biological treatment facility were quantified The bacteria were present in the biofilm throughout the year, suggesting that the biofilm of the biological treatment facility ... 0.005% of the total bacteria present in the biofilm, and their cell number increased during fall (Fig 3) Fig - Seasonal changes in the abundance ratio of microcystin-degrading bacteria in the biofilm...
- 9
- 522
- 0
Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... most of the experiments QZ drafted the manuscript AC and MW strictly revised the manuscript and the experiment design CX, DZ, RJ, QL, YZ, ZC and XC assisted with the experiments All of the authors ... in the x-axis, whereas the corresponding Ct values are presented on the y-axis The correlation coefficient and the slope value of the regression curve were calculated and indicated while the ... specificity, repeatability and reproducibility The sensitivity of the assay was used as the limit extent of detection when testing 10-fold diluted DNA standards in triplicate The dilution of plasmid pcDNA3.1-gC...
- 10
- 293
- 0
Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... RAB25, and CDH1 PC3 was negatively correlated with the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the ... (AD and SQ) and negative PC2 values (LC and SC) Because PC2 was positively correlated with the expression of CDH1, S100P, and RAB25, these genes may be significant in the classification of the ... data and the Kim et al data together, the remarkable and specific upregulation of S100P in AD cells suggests that this is a candidate marker for distinguishing the AD subtype Although the Kim and...
- 12
- 520
- 0
Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... addition to the loss of cell–cell adhesion, involves degradation of the extracellular matrix and release of cells from the constraints of cell–cell and cell–matrix interaction MMPs and urokinase-type ... suppressed the disruption of cell–cell adhesion, the scattered phenotype of the cells and the redistribution of E-cadherin into the cytoplasm (Fig 5C) C a b c d e f g h i j k l B Fig Involvement of Src ... disruption of E-cadherin-dependent cell–cell-adhesion and the expression of uPA are causally involved in tumor progression, the understanding of these underlying intracellular events is of importance...
- 14
- 599
- 0
báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... designing the qRT-PCR strategy GCS provided funding and helped designing the study and writing the paper PZ designed the qRT-PCR strategy, evaluated the gene expression data and wrote the paper ... reverse transcribed and amplified in the presence of primers and probes specific for β- actin house keeping gene and genes encoding different cytokines Expression of IFN-γ and IL-2 genes was significantly ... In line with these data, the extent of the increased expression of these genes was strictly dependent on the number of spiked gp100280–288 specific CTL Most importantly, these results indicate...
- 9
- 438
- 0
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final ... Quantification of viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm...
- 5
- 452
- 0
báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final ... Quantification of viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm...
- 5
- 539
- 0
Báo cáo khoa học: "Effects of Benzo[a]pyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells" doc
... phenol or TCDD at the concentrations shown The columns represent the means of triplicate cultures, and the bar on the columns represent the means±SD of triplicate cultures Effects of Benzo[a]pyrene, ... were apparent between the pollutant-treated and the vehicle-treated groups (Fig 2) Next, we addressed the question as to whether the administration of each pollutant at the reference dosage caused ... which were attributed to the model systems used for the experiments, these varied from the enhancement of induction to no change [1, 7, 16-18] In terms of the effects on IL-6 and TNFα production,...
- 8
- 420
- 0
Báo cáo y học: "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte" pptx
... contributed to the writing of the manuscript SMB conceived of the study, participated in its design and analysis, and prepared and revised the manuscript Both authors read and approved the final manuscript ... density of cells and prevalence of filamentous actin throughout the monolayer precluded any further quantitative analysis (data not shown) Effects of TNF-α and EGF on levels of aggrecan and type ... to the addition of EGF for the remainder of the 24 hours resulted in comparable decreases in levels of aggrecan and type II collagen mRNA (89 ± 2% and 81 ± 7%, respectively; data not shown) The...
- 12
- 388
- 0
Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
... expression of E-cadherin, α-, β- and γcatenin may persist into the later stages of breast carcinoma Siitonen et al and Oka et al found a correlation between loss of E-cadherin and the presence of nodal ... contributed to the conduct of the study, REM contributed to clinical follow ups and helped in editing the manuscript, WGJ contributed to the conduct of the study, design of primers and statistical ... difference in the expression of Ecahderin, α-, β- and γ-catenin between tumour and normal breast tissue Furthermore, measurement of expression of these molecules is not of prognostic value in predicting...
- 6
- 303
- 0
Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... infection, there were 2-5 folds increase in the expression of TNF-a and CCL-5/RANTES At hours post-H5N1/2004 infection, there were marked increase in the expression of CXCL-10/IP-10 and CCL-5/ ... in the stimulation of B lymphocytes for antibody production TNF-a together with IL-6 may boost proliferation and differentiation of B cells, and proliferation of T cells As a result, all these ... to the infiltration of inflammatory cells into the influenza infected respiratory tract Our results showed that H5N1 was a potent inducer of CXCL-10/IP-10 and CCL-5/RANTES The induction of these...
- 9
- 338
- 0
Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc
... CPS and CZhu carried out the molecular probe studies, and drafted the manuscript CZha participated in the design of the study and performed the statistical analysis SC conceived of the study, and ... copies/μL The sensitivity of the real-time PCR was compared with conventional PCR (Yue et al., 2009) Specificity of the real-time PCR To determine the specificity of the real-time PCR, the standard ... mix of 25 μL: 16.0 μL sterilized water, 2.5 μL of 10× buffer, 3.0 μL of dNTP, μL of each primer (NS1-FP and NS1-RP), μL of BQ strain DNA, and 0.5 μL of Ex Taq™ DNA Polymerase (Ex taq) The thermal...
- 4
- 587
- 0
báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... on the geometric mean of the expression values of the set of the control genes tested The lower the M value, the more stably expressed the gene is Also, the program enables the exclusion of the ... value of all the other genes and also a change in the M values of the unstable genes, BbrizGDP and BbrizUBI (Figure 3b) To check if the Figure Box-whisker showing the Ct variation of each candidate ... BbrizUBCE and BbrizTUB were again the more stable and the least stable genes respectively, while there was a slight difference in the order of stability of the other genes The second and third...
- 10
- 267
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25