Ứng dụng chỉ thị dna để định danh gene kháng bệnh sương mai ( late blight) ở một số giống dòng cà chua

45 RESULTS· SEQUENCE: , 5'- CAT GTC CAG TTG AGG AGC GA -3' COMPLEMENT: 5'- TCG CTC CTC AAC TGG ACA TG -3' LENGTH: 20 ' GC CONTENT: 55.0 % MELT TEMP: 57.0 oc MOLECULAR WEIGHT: 6182.1 g/mole EXTINCTION COEFFICIENT: 199500 L/(mole·cm) nmole/OD260: 5.01 ' pg/OD2&0: 30.99 ' i MELTING.TEMPERATURE SE1TINGS TARGETlYPE: DNA ·OLIGO CONC : Na CONC + · Mg+ + CONC dNTPsCONC 0.25 pM 50 mM monovalent salt O mM divalent salt mM nucleotide triphosphate C_fi� true thir efip (I:Iairp�) Structures ,., Structure Name " ' ,., ,-, _ 6G (kcalmolc"1) Tm("() •0.32 30.8 -16.9 -55.6 -0.08 26.2 -20.9 ·69.82 Cfiu true thir �dp (Self-Dimer) -'- ! �··.? �·,.;� JI � ,,, lr�-. -= ZTT-GJ.ZGJ.OCGA -_ _3 , -'· CAl'GT===cGA 11 :: 3' AGCGAGGAGTTGACCTGTAC 1111 3' �GACCTGTAC ' Oelta G •1,95 kcaVmole BasePairs ;' DebG C.64 kcaVmole , Base Pairs 5' 5' Ql'GTCCA '"TTGAGGAGCGA IU 11 : : :: • Oelt•G ·1.95 kc:aVmole ·, Base Pairs ' Delta G ·3.61 kcaVmole ' Base Pairs - CATGTCCAGnGA.GGAGCGA 3' AGCGAGGAGTTGACCTGTAC 3' A$ �GAGTTGACCtGTAC 13• -3.t4kcaVmole Oelta G ·1,95 kc:aVmole 8asePalrs Delta G -5.38 kcaVmole hsePAlrs Ill f::::_._ � Oelt•G ['' �-.GA , Haximum Delta G -37.9 kcaVmole 5' Output �GTCCA '"TTGAGGA&.'"GA II AGCGArnGAm : �-5, Cll-GT-= = -· - : II :: : : I 3• AGCGAGGAGTTGACCTGYAC Cfiu true thir efip (Hetero-Dimer) etia TG63 el� el� ... C_fi� true thir efip (I:Iairp�) Structures ,., Structure Name " ' ,., ,-, _ 6G (kcalmolc"1) Tm( "() •0.32 30.8 -16.9 -55.6 -0.08 26.2 -20.9 ·69.82 Cfiu true thir �dp (Self-Dimer) -'- !... WEIGHT: 6182.1 g/mole EXTINCTION COEFFICIENT: 199500 L/(mole·cm) nmole/OD260: 5.01 ' pg/OD2&0: 30.99 ' i MELTING.TEMPERATURE SE1TINGS TARGETlYPE: DNA ·OLIGO CONC : Na CONC + · Mg+ + CONC dNTPsCONC... AGCGArnGAm : �-5, Cll-GT-= = -· - : II :: : : I 3• AGCGAGGAGTTGACCTGYAC Cfiu true thir efip (Hetero-Dimer) etia TG63 el� el�