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DermFactor VẬT LIỆU SINH HỌC ĐIỀU TRỊ VẾT THƯƠNG, LOÉT, PHẪU THUẬT CẮT TRĨ

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Nhiễm trùng vết mổ, dùng cho các trường hợp bỏng, cấy ghép da, vết thương cấp tính, mãn tính, điều trị vết loét tiểu đường, loét tĩnh mạch. Loét xạ trị, Lazer. Loét do bỏng nặng Loét do bệnh lý mạch máu Loét sâu một phần hay toàn phần Vết thương do chấn thương lâu lành Nằm liệt, ngồi lâu ngày gây nên lỡ loét Vết mổ nhiễm trùng, vết phẫu thuật hậu môn Áp dụng cho việc hồi phục vết thương giữa giai đoạn thứ hai và thứ ba Loét chân, vết thương ở chân lâu lành do biến chứng của đái tháo đường (tiểu đường)

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Contents lists available atScienceDirect International Journal of Surgery journal homepage:www.elsevier.com/locate/ijsu Original Research

wound healing after anal surgery: A randomized study

Shuo Chena,1, Zhiguang Huanb,1, Lin Zhanga,∗, Jiang Changb,∗∗

a Department of Anorectal Surgery, Tianjin Union Medicine Centre, 190 Jieyuan Road, Tianjin 300121, China

b State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road,

Shanghai 200050, China

A R T I C L E I N F O

Keywords:

Silicate-based wound dressing

DermFactor®

Anal surgery

Wound healing

A B S T R A C T

Background: The object of the present study is to evaluate the feasibility of a silicate-based wound dressing (DermFactor®) in treating the wound of the patients after anorectal surgery

Materials and Methods: The present study included 328 patients who received anal surgery during the period from March 2013 to June 2015 The patients were randomized to 2 groups The patients (n = 162) in the control group received conventional dressing therapy, while those in the observation group (n = 166) were treated with the combination of conventional dressing therapy and the use of a silicate-based wound dressing (DermFactor®) The wound healing outcomes of the two groups were observed and compared with each other by statistical analysis

Results: The average healing cycles in the observation group were 19.04 days for combined hemorrhoid patients, 23.72 days for analfistula patients and 21.14 days for anal fissure patients, respectively, which were shorter than those in the control group (23.25 days for mixed hemorrhoid patients, 27.76 days for analfistula patients and 24.32 days forfissure in ano patients, respectively) In addition, the observation group presented a significantly higher effective rate (80.4%) than the control group (70.4%)

Conclusion: Our data demonstrated that the wound after anorectal surgery could be more effectively treated by using silicate-containing DermFactor®

1 Introduction

Anal diseases, including hemorrhoid, analfistula and anal fissure, is

common all over the world and affecting people in all age groups [1,2]

The patients suffering from these diseases frequently require surgical

treatment, and the post-operative care has been considered as one of

the most important factors that determine the cosmetic and functional

outcome of the anal organ [3]

Wound healing is an essential step during the procedure of

post-operative care after anal surgery [3] For example, it is believed that a

desirable wound healing could help reduce the post-operative pain after

haemorrhoidectomy, which quite commonly causes delayed patient

discharge [4] However, due to the unusual circumstance, the wound

healing after anal surgery is frequently delayed by postoperative

com-plications, such as bleeding, infection and edema, despite the use of

wound healing cream Therefore, it is still necessary to investigate more

efficient therapeutic regimen to accelerate the postoperative wound

healing after anal surgery

DermFactor®is a silicate-based wound dressing that has been widely used for wound treatment in cases of burn, diabetic foot and bedsore [5], and its bioactive effect on wound healing is based on the fact that silicate materials can stimulate the vascularization that is essential for healing of soft tissue [6] This important feature makes DermFactor®a potential candidate as a wound dressing to accelerate wound healing during the postoperative care after anal surgery Therefore, during the period from March 2013 to June 2015, DermFactor®was used to treat

166 patients, who received anal surgery, aiming at improving the wound healing This study presented the clinical outcome of the ap-plication of DermFactor®as wound dressing for anal surgery, and also evaluated its benefit in terms of wound healing rate and efficiency

https://doi.org/10.1016/j.ijsu.2018.02.036

Received 30 August 2017; Received in revised form 4 February 2018; Accepted 14 February 2018

∗ Corresponding author.

∗∗ Corresponding author.

1 The two authors contributed to the work equally.

E-mail addresses: xz2013l@126.com (L Zhang), jchang@mail.sic.ac.cn (J Chang).

Available online 23 February 2018

1743-9191/ © 2018 IJS Publishing Group Ltd Published by Elsevier Ltd All rights reserved.

T

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2 Materials and Methods

2.1 Patients

This study included 328 patients who received anal surgery at our

hospital from March 2013 to June 2015 The selection of the patient

was based on the following criteria:

The patients suffered from mixed hemorrhoid, anal fistula or fissure

in ano before receiving anal surgery

The patients were not allergic to any drug that was involved in this

study, and those suffering from serious mental sickness and

hematolo-gical diseases were excluded In addition, pregnant and lactating

tients were not included in this study The ages of all the selected

pa-tients in this study were over 18 years old All the papa-tients were fully

informed before voluntarily attending this study with the informed

consent signed precedent to the treatment

The patients (n = 328) randomized to 2 groups The patients

(n = 162) in the control group received conventional dressing therapy,

while those in the observation group (n = 166) were treated with the

combination of conventional dressing therapy and the use of

DermFactor® (Shanghai Gui-Jian Biomaterials Co., Ltd, Shanghai,

China) The patients' brief data was summarized and presented in

Table 1 The patient's data of the control group did not show significant

difference from that of the observation group, which allowed for

rea-sonable comparison between the groups

2.2 Methods of wound treatment

For the patients in the control group, the postoperative wound care

was carried out by means of routine sitz cleaning and dressing change

[7], until the wound was completely healed For the observation group,

the wound was first cleaned by means of routine sitz cleaning and

covered with the same dressing as that used in the control group

Subsequently, DermFactor®was sprayed on the wound area until it was

completely covered by the DermFactor®powder Finally, the wound

area was covered with axenic gauze After 24 h, the axenic gauze was

removed and the wound area was cleaned and observed for evaluation

of the wound healing progress The procedure as mentioned above was

repeated until the wound was completely healed

The affected areas of the patients were under close observation

during the postoperative care [8] Special attention was paid on the

surface area, exudate, color and healing time of the wound The total

follow up period for all the patients was set to 1 year Symptomatic

treatment was carried out in case that untoward reaction took place

2.3 Outcome measures

The criterion for the outcome measures in this study was as the

follows:

― The treatment was considered to be “significantly effective” (SE) if

the wound was completely cured

― The treatment was considered to be “effective” (E) if the cured part

of the wound surface area amounted to at least 50% of the overall wound surface

― It was noted as “improved” if 30%–50% of the wound was cured (IMP)

― It was noted as “ineffective” (INE) if the cured part of wound was less than 30%

The total effective rate (Et) was calculated according to Eq (1):

Et (%) NSE NE

where NSE and NE stand for the patients number of the SE and E groups after the treatment, and Nt is the total patients number

2.4 Statistical analysis All the data involved in this study was analyzed using SPSS 15.0 software The enumeration data was presented as number (n) and percentage (%), of which the comparison between the control and ob-servation was made using Chi-square test The measurement data was demonstrated as mean ± SD Comparisons between the measurement data of the control and observation groups was made using t-test

P < 0.05 was considered statistically significant

3 Results 3.1 The time of wound healing The wound healing of the patients in the control and observation groups was observed according to previous instruction [9], and the average healing time of the patients was presented in Fig 1 The average healing time of the patients in the observation was 21.86 days, which was significantly shorter than that in the control group (25.36 days, P < 0.05) For the patients suffering from different type of anal disease, the average healing time in the observation group (19.04, 23.72 and 21.14 days for mixed hemorrhoid, analfistula and fissure in ano, respectively) was also significantly lower than that in the control group (23.25, 27.76 and 24.32 days for mixed hemorrhoid, analfistula andfissure in ano, respectively)

3.2 Therapeutic effect After 21 days of treatment, the outcome in the control and ob-servation groups was measured and compared with each other, and the

Table 1

Patients' brief data.

Control group Observation group Patients number (n) 162 168

Average age (years) 43.5 ± 1.9 41.5 ± 2.3

Male/Female (n) 94/68 102/66

Type of disease

Mixed hemorrhoid (n) 65 78

Fissure in ano (n) 39 37 Fig 1 The wound healing time of the patients in the control and observation (* indicated

p < 0.05).

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results were presented inTable 2 No complication of wound healing

occurred in the patients In the control group, the number of the

pa-tients that were considered as SE, E, IMP and INE was 62, 52, 0 and 48,

respectively In contrast, the observation group has 74, 61, 0 and 33

patients who were consider as SE, E, IMP and INE, respectively The

number of SE and E is significantly higher than that in the control

group In addition, the observation group showed a higher total

effec-tive rate (80.4%) as compared with the control group (70.4%)

4 Discussion

Anal diseases, including mixed hemorrhoid, analfistula and fissure

in ano, are common disease all over the world, for which anal surgery is

a routine method of treatment However, the wound healing is a major

problem in the postoperative care of the anal surgery due to the fact

that the anal site is prone to bacterial infection The formation of

bacteriotoxin and enzyme induced by the bacterial infection could lead

to a series of serious consequence, such as necrosis of local tissue and

the dissolution of matrix and collagenfibrils [10], which subsequently

result in exacerbated tissue trauma and retarded wound healing In

addition, the wound exudation and increased necrotic tissue that are

induced by the infected wound could elevate the local strain in the

wound, which in turn allows for the diffusion of the infection and hence

hinders the wound healing process

Due to the unusual condition of the wound site in anal surgery as

above mentioned, the postoperative dressing change is crucial for the

wound healing, which is one of the most important factors that

de-termine the outcome of the treatment [11] However, the routine

treatment may not be adequate for desirable outcome of wound

healing In this study, it was clear that DermFactor® possessed the

ability of accelerating the wound healing process and improving the

therapeutic effectiveness, which was closely related to the

well-estab-lished physicochemical and biological characteristics of silicate-based

wound healing materials Containing silicate micro-particles as the

major component, DermFactor®was able to dissolve in a short period

once after contacting with the bodyfluid, when subsequently allowed

for the formation of a silica layer on the surface Due to its

character-istic surface chemistry, such a silica layer was capable of adsorbing the

surrounding biological molecules and hence accelerating the

penetra-tion of fibrocytes into the site [12] This process could consequently

contribute to the construction of collagen tissue along the surface of the

silicate-based micro-particles [13], and then a collagen matrix would be

formed in the wound site, which could consequently act as a temporary

extracellular matrix that supported the proliferation offibrocytes,

epi-cytes and endotheliocyte during the regeneration of soft tissue in the

wound site [14,15] The overall process enabled the accelerated wound

healing by the use of DermFactor®in this study, and the results of this

study suggested that DermFactor®is a beneficial wound dressing for the

postoperative care after anal surgery

5 Conclusion

In summary, we found in this study that the postoperative wound healing process of the patients after receiving anal surgery was sig-nificantly accelerated by the use of DermFactor®, which did not induce any adverse reaction to the patients Thereafter, the silicate based bioactive dressing DermFactor®seems to be an ideal supplement to the current routine postoperative wound care that would contribute to improved clinical outcome and patients comfort

Ethical approval The study protocol and the patient informed consent was reviewed and approved by the study review board in People's Hospital of Tianjin prior to study initiation

Author contribution Shuo Chen and Lin Zhang carried out the design, participated all the experiments andfinished the data collections Shuo Chen and Zhiguang Huan coordinately analyzed experimental data and prepared the manuscript Jiang Chang proposed the study, reviewed the experi-mental data and the manuscript and carried out the revision of the manuscript prior to the submission All authors have read and approved the content of the manuscript

Conflicts of interest None

Research Registration Unique Identifying Number (UIN) UIN: researchregistry2473

Guarantor Jiang Chang

Acknowledgment This study was supported by The National Key Research and Development Program of China (2016YFC1100201) and Shanghai S&T Innovation Action Plan Project (16441904500)

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Table 2

Comparison between the therapeutic effect in the control and observation group after 21

days of treatment.

Control group Observation group Total patients number (n) 162 168

Therapeutic effect

OE (patients number, n) 62 74

E (patients number, n) 52 61

IMP (patients number, n) 0 0

INE (patients number, n) 48 33

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acute soft tissue injury in experimental animal research progress, Chin J Exp Trad.

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[14] S.N Feng, S.L Wang, F.Q Zhu, H Huang, B Zhang, J.X Jiang, Z.G Wang, Stromal cell derived factor-1 alpha promotes neovacsularization during cutaneous wound healing, J Clin Rehabilitative Tissue Eng Res 13 (2009) 2019–2023 [15] P Saravanapavan, J.R Jones, S Verrier, R Beilby, V.J Shirtliff, L.L Hench, J.M Polak, Binary CaO-SiO2 gel glasses for biomedical applications, Bio Med Mater Eng 14 (2004) 467–486

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