NGHIÊN CỨU, TỔNG HỢP, ĐẶC TRƯNG VẬT LIỆU LAI VÔ CƠ (Ag, Fe3O4) HỮU CƠ (CHITOSAN) CẤU TRÚC NANO ĐỊNH HƯỚNG ỨNG DỤNG TRONG Y SINH

NGUYỄN THỊ NGOAN NGHIÊN CỨU, TỔNG HỢP, ĐẶC TRƯNG VẬT LIỆU LAI VÔ CƠ (Ag, Fe3O4) - HỮU CƠ (CHITOSAN) CẤU TRÚC NANO ĐỊNH HƯỚNG ỨNG DỤNG TRONG Y SINH BÌNH ĐỊNH - 2017 NGUYỄN THỊ NGOAN VIỆN HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM HỌC VIỆN KHOA HỌC VÀ CÔNG NGHỆ NGHIÊN CỨU, TỔNG HỢP, ĐẶC TRƯNG VẬT LIỆU LAI VÔ CƠ (Ag, Fe3O4) - HỮU CƠ (CHITOSAN) CẤU TRÚC NANO ĐỊNH HƯỚNG ỨNG DỤNG TRONG Y SINH LUẬN ÁN TIẾN SỸ HÓA HỌC Chuyên ngành: Vật liệu cao phân tử tổ hợp Mã số: 62440125 Người hướng dẫn khoa học: PGS.TS Trần Đại Lâm PGS.TS Nguyễn Tuấn Dung BÌNH ĐỊNH - 2017 Tôi xin cam đoan nội dung luận án thực hướng dẫn người hướng dẫn khoa học Các số liệu, kết trình bày luận án trung thực chưa công bố luận án khác Tác giả luận án NCS Nguyễn Thị Ngoan Trước hết xin trân trọng bày tỏ lòng biết ơn sâu sắc đến PGS.TS Trần Đại Lâm, PGS.TS Nguyễn Tuấn Dung tận tình hướng dẫn suốt trình hoàn nghiên cứu hoàn thiện luận án Tôi xin gửi lời cảm ơn sâu sắc đến PGS.TS Phạm Gia Điền tập thể phòng Công nghệ chất có Hoạt tính sinh học - Viện hóa học; Phòng Nghiên cứu ứng dụng triển khai công nghệ - Viện Kỹ thuật nhiệt đới; GS Jeung Sang Go phòng thí nghiệm trọng điểm NExT MEMS - Trường Đại học Pusan - Hàn Quốc tạo điều kiện giúp hoàn thành nhiệm vụ nghiên cứu hoàn thiện luận án Tôi xin gửi lời cảm ơn chân thành đến phòng sau đại học Viện Kỹ thuật Nhiệt đới, Khoa Hóa học - Học viện Khoa học Công nghệ - Viện Hàn lâm Khoa học Công nghệ Việt Nam tạo điều kiện để hoàn thiện luận án Xin gửi lời cảm ơn đến gia đình, bạn bè động viên suốt trình thực luận án! Tác giả luận án Nguyễn Thị Ngoan MỤC LỤC CHƯƠNG CÁC PHƯƠNG PHÁP NGHIÊN CỨU VÀ THỰC NGHIỆM 40 1.1 1.2 1.3 AgNPs Bs Ca CS CS/NPs CS/AgNPs1 CS/AgNPs2 CS/AgNPs3 CS/MNPs CS/MNPs1 CS/MNPs2 CS/MNP3 CS-FITC DANH MỤC CÁC CHỮ VIẾT TẮT 1.4 Nano bạc 1.5 Bacillus subtilis Candida albicans Chitosan 1.6 Nanocompozit chứa nano kim loại chitosan Nanocompozit chứa nano bạc chitosan chế tạo phương pháp khử NaBH 1.7 Nanocompozit chứa nano bạc chitosan chế tạo 1.8 phương pháp dùng chitosan điều kiện gia nhiệt 1.9 Nanocompozit chứa nano bạc chitosan chế tạo 1.10 phương pháp dùng chitosan lò vi sóng 1.11 Nanocompozit chứa nano sắt từ chitosan 1.12 Nanocompozit chứa nano sắt từ chitosan chế tạo 1.13 phương pháp đồng kết tủa dòng nitơ trơ 1.14 Nanocompozit chứa nano sắt từ chitosan chế tạo 1.15 phương pháp đồng kết tủa hệ vi lưu 1.16 Nanocompozit chứa nano sắt từ chitosan chế tạo 1.17 phương pháp oxy hóa kết tủa 1.18 Chitosan biến tính với florescien isothiocyanat 1.19 Curcumin 1.20 Diclometan 1.21 Độ đề axetyl hóa Cur 1.22 Doxorubicin DCM 1.23 Escherichia coli DD 1.24 Phương pháp phổ tán xạ lượng Hiển vi điện tử quét xạ DOX Ec EDX trường 1.25 Nanocompozit nano sắt từ chitosan gắn chất phát quang FESEM 1.26 Fluorescein isothiocyanat FMNPs 1.27 Nồng độ ức chế tối thiểu 50% vi khuẩn, vi nấm FITC IC50 1.1 L a 1.3 M NP 1.5 M S 1.7 P a 1.9 S a 1.11 S e 1.13 S E 1.15 T E 1.17 T LC 1.19 X RD 1.21 U V1.23 V S 1.25 1.28 1.2 Lactobacillus fermentum 1.4 Nano sắt từ Fe3Ơ4 1.6 1.8 1.10 Từ độ bão hòa Pseudomonas aeruginosa Staphylococcus aureus 1.12 Salmonella enteric 1.14 Hiển vi điện tử quét 1.16 Hiển vi điện tử truyền qua 1.18 Sắc ký lớp mỏng 1.20 Nhiễu xạ tia X 1.22 thấy 1.24 Hấp thụ ánh sáng tử ngoại vùng nhìn Từ kế mẫu rung 1.29 DANH MỤC CÁC BẢNG 1.30 1.31 1.32 DANH MỤC CÁC HÌNH 1.33 1.34 1.35 1.36 MỞ ĐẦU Công nghệ vật liệu nano lĩnh vực mẻ thu hút quan tâm nhà khoa học Nhiều thành tựu ứng dụng ngành vật liệu điện tử, quang điện tử, vật liệu từ, y sinh học nghiên cứu phát triển thành công [6, 83, 87] 1.37 Vật liệu polyme nanocompozit, kết kết hợp polyme vật liệu nano, thể ưu điểm thành phần chất gia cường nano pha phân tán polyme Lý thuyết, mô hình chế tạo ứng dụng loại vật liệu quan tâm ý Ngoài ứng dụng vượt trội vật liệu polyme nanocompozit lĩnh vực xây dựng, điện tử, gia dụng tính chất lý trội vật liệu nanocompozit tăng độ bền, chịu nhiệt, giảm độ thấm khí Ngày nay, vật liệu nanocompozit quan tâm cho ứng dụng sinh học dựa vào khả tương thích sinh học phân hủy sinh học số loại polyme sinh học kết hợp với tính đặc biệt tính chất từ, tính chất quang chất gia cường nano 1.38 Trong số polyme sinh học, chitosan thu hút quan tâm nhà nghiên cứu [5, 9, 70, 86] Chitosan sản phẩm đề axetyl hóa chitin, có nguồn gốc từ phế phẩm ngành chế biến thủy hải sản, polyme có hàm lượng đứng thứ tự nhiên (sau xenlulo) [86] Chitosan mang đầy đủ đặc trưng ưu việt chitin như: (i) có tính tương thích sinh học không độc hại, (ii) có khả phân hủy sinh học, (iii) có tính hấp phụ cao 1.39 Thêm vào đó, chất gia cường nano hạt nano bạc, nano sắt từ chế tạo ứng dụng thành công ứng dụng lĩnh vực y sinh, chẩn đoán điều trị bệnh mang tính chất từ tính chất quang tính kháng khuẩn thú vị [1, 7, 15, 28, 32, 46, 97] 1.40 Vật liệu polyme nanocompozit kết hợp chất gia cường nano bạc, nano sắt từ polyme chitosan hứa hẹn tăng cường ứng dụng loại vật liệu lĩnh vực y sinh 1.41 Chính mục tiêu luận án chế tạo vật liệu nanocompozit đa chức chứa nano bạc, nano sắt từ chitosan nhằm kết hợp tính chất quý báu riêng rẽ vật liệu thành phần, tạo hệ polyme nanocompozit đa chức có Trần Thị Luyến Nguyễn Huỳnh Duy Bảo, (2000) Hoàn thiện quy trình sản xuất chitin - chitosan chế biến số sản phẩm công nghiệp từ phế liệu vỏ tôm, cua Báo cáo khoa học, đề tài cấp bộ, trường Đại Học Thủy sản 1.523 r 1.524 rp i X • • /V /V _ A _ 1.525 Tài liệu tiêng Anh 10 Alexandre Luis ParizeI Hellen Karine Stulzer; Mauro César Marghetti Laranjeira; Inês Maria da Costa Brighente; Tereza Cristina Rozone de Souza, (2012), Evaluation of chitosan microparticles containing curcumin and crosslinked with sodium tripolyphosphate produced by spray drying, Quím Nova, 35, 11 Alireza Alishahi, Mohammed Alder, (2012), Applications of Chitosan in the Seafood Industry and Aquaculture: A Review, Food and Bioprocess Technology, 5, 3, 817-830 12 Andreas Bernkop-Schnurch, Sarah Dunnhaupt, (2012), Chitosan - based drug delivery systems, European Journal of Pharmaceutics and Biopharmaceutics, 81, 3, 463-469 13 An-Hui Lu, E L Salaba, Ferdi Schuth, (2007), Magnetic Nanoparticles: Synthesis, Protection, Functionalization, and application, Angewandte Chemie International Edition, 46, 8, 1222-1244 14 Anumita Chaudhury, Surajit Das, (2011), Recent Advancement of Chitosan- Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents, AAPS PharmSciTech.; 12(1): 10-20 15 A A Kudrinskiy, A Yu Ivanov, E V Kulakovskaya, A I Klimov, P M Zherebin, D V Khodarev, Anh-Tuan Le, Le Thi Tam, G V Lisichkin, Yu A Krutyakov (2014), The Mode of Action of Silver and Silver Halides Nanoparticles against Saccharomyces cerevisiae Cells Journal of Nanoparticles, 16 Bahareh Khodashenasa, Hamid Reza Ghorbanib, (2015), Synthesis of silver nanoparticles with different shapes, Arabian Journal of Chemistry In Press, Corrected Proof, Available online January 17 Bhawana, Basniwal RK, Buttar HS, Jain VK, Jain N, (2011) Curcumin nanoparticles: preparation, characterization, and antimicrobial study, J Agric Food Chem 9; 59(5):2056-61 18 Bo Duan, Feng Liu, Meng He, Lina Zhang, (2014), Ag-Fe 3O4 nanocomposites@chitin microspheres constructed by in situ one-pot synthesis for rapid hydrogenation catalysis, Green Chemistry, 16 (5), 2835-2845 19 Begum Akduman Begum Akduman, Murat Uygun (2013), Fe3Ơ4 magnetic core coated by silver and functionalized withN-axetyl cysteine as novel nanoparticles in ferritin adsorption, JNanopart Res, 15:1564 20 Bhupendra Chudasama, Anjana K Vala, Nidhi Andhariya, R V Upadhyay, R V Mehta (2010), Enhanced antibacterial activity of bifunctional Fe3Ơ4-Ag core-shell nanostructures, Nano research, 2, (12), 955-965 21 Boca-Farcau S, Potara M, Simon T, Juhem A, Baldeck P, Astilean S, (2014), Folic acid-conjugated, SERS-labeled silver nanotriangles for multimodal detection and targeted photothermal treatment on human ovarian cancer cells, MolPharm 3; 11(2): 391-9 22 Bouazza, S., Alonzo, V., & Hauchard, D (2009) Synthesis and characterization of Ag nanoparticles-polyaniline composite powder material, Synth Metals, 159, 1612-1619 23 B He, J Tan, K Liew, H Liu, (2004), Synthesis of size controlled Ag nanoparticles, J Mol Catal Chem., 221, 121-126 24 Chen, L Y and Subirade, M., (2005), Chitosan/B-lactoglobulin core-shell nanoparticles as nutraceutical carriers Biomaterials, 26, 6041-6053 25 Chignell CF, Bilski P, Reszka KJ, Motten AG, Sik RH, Dahl TA, (1994), Spectral and photochemical properties of curcumin, Photochem Photobiol.; 59(3):295-302 26 Chuah LH, Billa N, Roberts CJ, Burley JC, Manickam S, (2013), Curcumincontaining chitosan nanoparticles as a potential mucoadhesive delivery system to the colon, Pharm Dev Technol.; 18(3):591-9 27 Cornell R.M, Schwertmann U (2003), The ion oxide: structure, properties, reaction, occurences, and uses, WILEY - vhc, Germany 28 Dao Thi Thuy Nguyet, Nguyen Phuc Duong, Le Thanh Hung, Than Duc Hien, Takuya Satoh (2011), Crystallization and magnetic behavior of nanosized nickel ferrite prepared by citrate precursor method Journal of Alloys and Compounds, 509 (23), 6621-6625 29 Dang Thi My Dung, Le Thi Thu Tuyet, Fribourg-Blanc Eric, Dang Mau Chien (2012), Influence of surfactant on the preparation of silver nanoparticles by polyol method Advances in Natural Sciences: Nanoscience and Nanotechnology, 3(3), 035004 30 Ding Hao Ding Hao, Shen Cheng-min, Hui Chao, Xu Zhi-chuan, Li Chen, Tian Yuan, Zhi Xue-Zhao, and Gao Hong-Jun, (2010), Synthesis and properties of Au- Fe 3O4 and Ag- Fe3O4 heterodimeric nanoparticles, Chin Phys B, 19, 6, 066102 31 DPatel V M., Prajapati B G., Patel M M., (2007), Design and characterization of chitosan containing mucoadhesive buccal patches of propranolol hydrochloride Acta Pharm., 57, 61-72 32 Dung Tran Thi Ngoc, Buu Ngo Quoc, Quang Dang Viet, Ha Huynh Thi, Bang Le Anh, Chau Nguyen Hoai, Ly Nguyen Thi, Trung Nguyen Vu, (2009) Synthesis of nanosilver particles by reverse micelle method and study of their bactericidal properties Journal of Physics: Conference Series, 187(1), 012054 33 Fabrega J, Luoma SN, Tyler CR, Galloway TS, Lead JR, (2011), Silver nanoparticles: behaviour and effects in the aquatic environment, Environ Int.; 37(2):517-31 34 Gavhane Yogeshkumar N., Gurav Atul S and Yadav Adhikrao V., (2013), Chitosan and Its Applications: A Review of Literature, International Journal of Research in Pharmaceutical and Biomedical Science, (1), 312-321 35 Gogoi N, Chowdhury D., (2014), In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties, JNanosci Nanotechnol.; 14(6):4147-55 36 Guichuan Xing, Jiang Jiang, Jackie Y Ying, and Wei Ji, (2010), Fe 3O4-Ag nanocomposites for optical limiting: broad temporal response and low threshold, Optics Express, 18, 6, 6183-6190 37 Gupta P, Bajpai M, Bajpai SK (2008) Investigation of antibacterial properties of silver nanoparticle-loaded poly (acrylamide-coitaconic acid)-grafted cotton fabric J Cotton Sci 12:280-286 38 H Bokura and kobayashi, (2003), Chitosan decreases total cholesterol in women: a randomized, double-blind, placebo-controlled trial, European Journal of Clinical Nutrition, 57, 721-725 39 Ha Phuong Thu, Le Thi Thu Huong, Hoang Thi My Nhung, Nguyen Thi 1.526 Tham, Nguyen Dac Tu, Ha Thi Minh Thi, Pham Thi Bich Hanh, Tran Thi Minh Nguyet, Nguyen Thi Quy, Pham Hong Nam, Tran Dai Lam, Nguyen Xuan Phuc, Duong Tuan Quang, (2011), Fe3O4/o-Carboxymethyl 1.527 Chitosan/Curcumin-based Nanodrug System for Chemotherapy and Fluorescence Imaging in HT29 Cancer Cell Line Chemistry Letters, 40(11), 12641266 40 Haizhen Huanga, Qiang Yuanb, Xiurong Yanga, (2004), Preparation and characterization of metal-chitosan nanocomposites, Colloids and Surfaces B: Biointerfaces, 39 (1-2), 31-37 41 Hongshui Wanga, Xueliang Qiaoa, Jianguo Chena, Shiyuan Ding, (2005), Preparation of silver nanoparticles by chemical reduction method, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 256 (2-3) , 111-115 42 Hu Q, Li B, Wang M, Shen J, (2004), Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: a potential material as internal fixation of bone fracture, Biomaterials.; 25(5):779-85 43 Hui Liu, Yumin Du, Xiaohui Wang, Liping Sun,(2004), Chitosan kills bacteria through cell membrane damage, International Journal of Food Microbiology, 95, (2), 147-155 44 Hui-Chia Yang and Min-Hsiung Hon, Preparation and characterization of magnetic chitosan nanoparticles and its encapsulation efficiency for Fluorouracil Chinese jounal of Science, 31, 10pp 45 Hui Wang Hui Wang, Jing Shen, Guixin Cao, Zheng Gai, (2012), Multifunctional PEG encapsulated Fe3O4@silver hybrid nanoparticles: antibacterial activity, cell imaging and combined photothermo/chemo-therapy, Journal of Materials Chemistry B 1, 6225-6234 46 Humberto H Lara, Nilda V Ayala-Nunez, Liliana Ixtepan-Turrent, Cristina RodriguezPadilla, (2010), Mode of antiviral action of silver nanoparticles against HIV-1, Journal of Nanobiotechnology, 8:1 47 H.El Ghandoor, H M Zidan, Mostafa M.H Khalil and M I M Ismail, (2012), Synthesis and Some Physical Properties of Magnetite (Fe 3O4) Nanoparticle, Int J Electrochem Sci., 7, 5734 - 5745 48 Inmaculad Aranaz, Marian Mengibar, Ruth Harris, Inés Panos, Beatriz Miralles, Niuris Acosta, Gemma Galed, Angles Heras, (2009), Function Characterization of chitin and chitosan, Current chemical Biology, 3, 200 - 230 49 Jan SS, Liu DC, Dong XY, Hu YM, Chen JD, (2008), Effects of chitosan and its derivative added to water on immunological enhancement and disease control, Mmunotherapy 4(7):697-701 50 Jeffrey N Anker, W Paige Hall1, Olga Lyandres, Nilam C Shah, Jing Zhao & Richard P Van Duyne (2012), Biosensing with plasmonic nanosensors, Nature Materials, 7, 442 - 453 51 Jiang Jiang, Hongwei Gu, Huilin Shao, Eamonn Devlin,Georgia C Papaefthymiou, Jackie Y Ying (2008), Bifunctional Fe 3O4-Ag Heterodimer Nanoparticles for TwoPhoton Fluorescence Imaging and Magnetic Manipulation, Advanced Materials, Volume 20, issue 23, 4403-4407 52 Ji-Ho Park, Geoffrey von Maltzahn, Lianglin Zhang, Michael P Schwartz, Sangeeta N Bhatia, Erkki Ruoslahti, and Michael J Sailor, (2008), “Magnetic Iron Oxide Nanoworms for Tumor Targeting and Imaging, Adv Mater (Cover Article) 20, 16301635 53 Khalid Z Elwakeel, (2010), Environmental Application of Chitosan Resins for the Treatment of Water and Wastewater: A Review, Journal of Dispersion Science and Technology, 31, 3, 273 - 288 54 Karla M Gregorio-Jauregui, Ma Guadalupe Pineda, Jorge E Rivera-Salinas, Gil Hurtado, Hened Saade, José L Martinez, Anna Ilyina, and Raúl G Lopez (2012), One-Step Method for Preparation of Magnetic Nanoparticles Coated with Chitosan, Journal of Nanomaterials, 14(10): 19636-19650 55 Ka§ HS, (1997), Chitosan: properties, preparations and application to microparticulate systems J Microencapsul 14(6):689-711 56 Khokon MA, Uraji M, Munemasa S, Okuma E, Nakamura Y, Mori IC, Murata Y., (2010), Chitosan-Induced Stomatal Closure Accompanied by Peroxidase- Mediated Reactive Oxygen Species Production in Arabidopsis, Bioscience, Biotechnology, and Biochemistry, 74, 11 57 Kong L, Gao Y, Cao W, Gong Y, Zhao N, Zhang X (2005), Preparation and characterization of nano-hydroxyapatite/chitosan composite J Biomed Mater Res, Part A 75A:275-282 58 K Vellingiri, T Ramachandran, M Senthilkumar, (2013), Eco-friendly Application of Nano Chitosan in Antimicrobial Coatings in the Textile Industry, Nanoscience and Nanotechnology, 3(4): 75-89 59 Lam Dai Tran, Nhung My T Hoang, Trang Thu Mai, Hoang Vinh Tran, Ngoan Thi Nguyen, Thanh Dang Tran, Manh Hung Do, Qui Thi Nguyen, Dien Gia Pham, Thu Phuong Ha, Hong Van Le, Phuc Xuan Nguyen (2010), Nanosized magnetofluorescent Fe3O4-curcumin conjugate for multimodal monitoring and drug targeting Colloids and Surfaces A: Physicochemical and Engineering Aspects 371: 104-12 60 Le Bin Ho, Tran Nguyen Lan, Tran Hoang Hai (2013), Monte Carlo simulations of core/shell nanoparticles containing interfacial defects: Role of disordered ferromagnetic spins Physica B: Condensed Matter, 430(0), 10-13 61 Le Thi Tam, Vu Ngoc Phan, Hoang Lan, Nguyen Thanh Thuy, Tran Minh Hien, Tran Quang Huy, Nguyen Van Quy, Huynh Dang Chinh, Le Minh Tung, Pham Anh Tuan, Vu Dinh Lam, Anh-Tuan Le (2013), Characterization and antimicrobial activity of silver nanoparticles prepared by a thermal decomposition technique Applied Physics A 113(3), 613-621 62 Le Anh-Tuan, Le Thi Tam, Nguyen Van Quy, Tran Huy Hoang, Dang Duc Anh, Tran Quang Huy, Vu Dinh Lam (2012), Powerful colloidal silver nanoparticles for the prevention of gastrointestinal bacterial infections 1.528 Advances in Natural Sciences: Nanoscience and Nanotechnology, 3(4), 045007, 10pp 63 Liang Guo, Guang Liu, Ruo-Yu Hong, Hong-Zhong Li, (2010), Preparation and Characterization of Chitosan Poly(acrylic acid) Magnetic Microspheres, Mar Drugs, 8, 2212-2222 64 Liang X.J., H.W Shi, X.C Jia, Y.X Yang, and X.N Liu (2011), Dispersibility, shape and magnetic properties of nano-Fe3O4 particles, Materials Sciences and Applications, 2, 1644-1653 65 Majeti N.V Ravi Kumar, (2000), A review of chitin and chitosan applications, Reactive and Functional Polymers, 46, 1, 1-27 66 Manfred Hesse, Herbert Meier, Bernd Beeh, (2007), Spectroscopic Methods in Organic Chemistry, 2nd Edition 67 Mansor Bin Ahmad, Jenn Jye Lim, Kamyar Shameli, Nor Azowa Ibrahim and Mei Yen Tay, (2011), Synthesis of Silver Nanoparticles in Chitosan, Gelatin and Chitosan/Gelatin Bionanocomposites by a Chemical Reducing Agent and Their Characterization, Molecules, 16, 7237-7248 68 Mansour Ghaffari-Moghaddam, Hassan Eslahi, (2014), Synthesis, characterization and antibacterial properties of a novel nanocomposite based on polyaniline/polyvinyl alcohol/Ag, Arabian Journal of Chemistry, 7, 5, 846855 69 Maragoni Venkatesham, Dasari Ayodhya, Alle Madhusudhan, Nagati Veera Babu, Guttena Veerabhadram, (2014), A novel green one-step synthesis of silver nanoparticles using chitosan: catalytic activity and antimicrobial studies, 4, 1, 113-119 70 Marguerite Rinaudo, (2006) Chitin and chitosan: Properties and applications, Prog Polym Sci 31, 603-632 71 Menon VP, Sudheer AR, (2007), Antioxidant and anti-inflammatory properties of curcumin, Adv Exp Med Biol.; 595:105-25 72 Mila Tejamaya , Isabella Romer , Ruth C Merrifield , and Jamie R Lead, (2012), Stability of Citrate, PVP, and PEG Coated Silver Nanoparticles in Ecotoxicology Media, Environ Sci Technol., 46 (13), 7011-7017 73 Mhurchu CN, Poppitt SD, McGill AT, Leahy FE, Bennett DA, Lin RB, Ormrod D, Ward L, Strik C, Rodgers A, (2004), The effect of the dietary supplement, Chitosan, on body weight: a randomised controlled trial in 250 overweight and obese adults, Int J Obes Relat Metab Disord.; 28(9): 1149-56 74 Murakami K et Al (2010), Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings, Biomater 3, 83-9 75 M.Kooti, S Saiahi, H Motamedi (2013), Fabrication of silver-coated cobalt ferrite nanocomposite and the study of its antibacterial activity, Journal of Magnetism and Magnetic Materials, 333, 138-143 76 M.Pradeepa, J.Jerlin Showmya and N Geetha, (2006), Preparation and Characterization of Silver Nanoparticles Impregnated With Chitosan Scaffolds, Plan cell biotechnology and molecular biology, 12, 1-2 77 Nataporn Sowasod, Tawatchai Charinpanitkul, Wiwut Tanthapanichakoon (2012), Nanoencapsulation of Curcumin in biodegradable Chitosan via Multiple Emulsion/ solvent evaporation, Bangkok journal 78 Ngo Thanh Hieu, Tran Dai Lam, Do Hung Manh, Tran Vinh Hoang, Le Van Hong, Nguyen Xuan Phuc (2010), Facile and solvent-free routes for the synthesis of sizecontrollable Fe3O4 nanoparticles Advances in Natural Sciences: Nanoscience and Nanotechnology, 1(3), 035001, 7pp 79 Nguyen Hoang Hai, Nguyen Chau, Nguyen Hoang Luong, Nguyen Thi Van Anh, Phan Tuan Nghia (2008), Applications of Magnetite Nanoparticles for Water Treatment and for DNA and Cell Separation Journal of Korean Physical Society, 53(3), 1601-1606 80 Nguyen T K Thanh, N Maclean , and S Mahiddine (2014), Mechanisms of Nucleation and Growth of Nanoparticles in Solution, Chem Rev., 114 (15), 76107630 81 N D Phu, D T Ngo, L H Hoang, N H Luong, N Chau, N H Hai, (2011), Crystallization process and magnetic properties of amorphous iron oxide nanoparticles Journal of Physics D: Applied Physics., 44(34), 345002 82 N Tresilwised, P Pithayanukul and C Plank, (2005), Factors Affecting Sizes of Magnetic Particles Formed by Chemical Co-precipitation, Mahidol University Journal of Pharmaceutical Sciences; 32(3-4): 71-76 83 Ov Salata, (2004), Applications of nanoparticles in biology and medicine, Journal of Nanobiotechnology, 2:3 84 Ping Gong, Huimin Li, Xiaoxiao He, Kemin Wang, Jianbing Hu, Weihong Tan, Shouchun Zhang and Xiaohai Yang, Preparation and antibacterial activity of Fe3O4@Ag nanoparticles, Nanotechnology, 18, 285604 85 Pham Hoai Linh, Do Hung Manh, Tran Dai Lam, Le Van Hong, Nguyen Xuan Phuc, (2011), Magnetic nanoparticles: study of magnetic heating and adsorption/desorption for biomedical and environmantal application International Journal of Nanotechnology, 8(4), 399-415 86 Prakip Kumar Dutta, Joydeep dutta, V S Tripathi (2004), Chitin and chitosan properties and application, Journal of Scientific & Industrial Research, 63, 20 - 31 87 P RangaReddy, K MohanaRaju, N SubbaramiRedd, (2012), A Review on Polymer nanocomposites: Monometallic and Bimetallic Nanoparticles for Biomedical, Optical and Engineering Application, Chemical Science Review and Letters 1(4), 228-235 88 P Przybylski, M Ratajczak-Sitarz, A Katrusiak, W Schilf, G Wojciechowski, B Brzezinski (2003), Crystal Structure of Schiff Base Derivative of Gossypol with 3,6,9-trioxa-decylamine Journal of Molecular Structure, 655: 293-300 89 Quang Ton That, Kim Phi Phung Nguyen, Poul Erik Hansen (2005), Schiff bases of Gossypol: an NMR and DFT study, Magnetic Resonance in Chemistry, 43, 4, 302-308 90 Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM (2008) Antibacterial characterization of silver nanoparticles against E coli J Mater Sci Technol, 24(2):192196 91 Reason Wilken, Mysore S Veena, Marilene B Wang, and Eri S Srivatsan, (2011), Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma, Mol Cancer.; 10: 12 92 Rhim J-W, Hong S-I, Park H-M, Perry Ng KW (2006) Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity JAgric Food Chem, 54: 5814-5822 93 Robert Prucek, Jin Tucek, Martina Kilianová, Ales Panácek, Libor Kvítek, Jan Filip, Milan Kolár, Katerina Tománková, Radek Zboril, (2011), The targeted antibacterial and antifungal properties of magnetic nanocomposite of iron oxide and silver nanoparticles Biomaterials, 32(21), 4704-4713 94 Sang Woo Kim, Jin Hee Jung, Kabir Lamsal, Yun Seok Kim, Ji Seon Min, and Youn Su Lee (2012), Antifungal Effects of Silver Nanoparticles (AgNPs) against Various Plant Pathogenic Fungi, Mycobiology; 40(1): 53-58 1.529 95.Savita Bisht, Georg Feldmann, Sheetal Soni, Rajani Ravi, Collins Karikar, Amarnath Maitra, Anirban Maitra, (2007), Polymeric nanoparticle- encapsulated curcumin ("nanocurcumin"): a novel strategy for human cancer therapy, Journal of Nanobiotechnology, 5:3 96 Schwertmann U, Cornell R.M (1991), Irons oxides in the laboratory preparation and characterization, Wiley - VHC, Germany 97 Senyei A, Widder K and Czerlinski C (1978), Magnetic guidance of drug carrying microspheres, J Appl Phys.49 3578-83 1.530 98.Sun Sheng-Nan, Wei Chao, Zhu Zan-Zan, Hou Yang-Long, Subbu S Venkatraman and Xu Zhi-Chuan (2014), Magnetic iron oxide nanoparticles: Synthesis and surface coating techniques for biomedical applications, Chinese Phys B 23: 037503 99 Susan W.P, Wijnhoven, Willie J.G.M, Peijnenburg, Carla A, Herberts, Werner I, Hagen (2009), Nano-silver a review of available data and knowledge gaps in human and environmental risk assessment, Nanotoxicology; 3(2): 109-138 100 S Govindan, E A K Nivethaa, R Saravanan, V Narayanan, A Stephen (2012), Synthesis and characterization of chitosan-silver nanocomposite, Applied Nanoscience, 2, 3, 299-303 101 S.Link and El-Sayed, (1999), Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles, J Phys Chem B, 103, 4212 - 4217 102 S Mohammadi-Samani, R Miri, M Salmanpour, N Khalighian, S Sotoudeh, and N Erfani (2013), Preparation and assessment of chitosan-coated superparamagnetic Fe3O4 nanoparticles for controlled delivery of methotrexate, Res Pharm Sci 8(1): 2533 103 Tai Thien Luong, Thu Phuong Ha, Lam Dai Tran, Manh Hung Do, Trang Thu Mai, Nam Hong Pham, Hoa Bich Thi Phan, Giang Ha Thi Pham, Nhung My Thi Hoang, Quy Thi Nguyen, Phuc Xuan Nguyen, (2011), Design of carboxylated Fe3O4/poly(styrene-co-acrylic acid) ferrofluids with highly efficient magnetic heating effect Colloids and Surfaces A: Physicochemical and Engineering Aspects, 384(1-3), 23-30 104 Tran Dai Lam, Le Van Hong, Pham Hoai Linh, Hoang Thi My Nhung, Nguyen Thi Quy, Luong Thien Tai, Ha Phuong Thu, Nguyen Xuan Phuc (2010), Biomedical and environmental applications of magnetic nanoparticles Advances in Natural Sciences: Nanoscience and Nanotechnology, 1(4), 045013, 5pp 105 Tran Thi Viet Nga, Nguyen Phuc Duong, Than Duc Hien, Composition and magnetic studies of ultrafine Al-substituted Sr hexaferrite particles prepared by citrate sol-gel method Journal of Magnetism and Magnetic Materials, 2012 324(6), 11411146 106 T.D.Lam, V.D.Hoang, L.N.Lien, N.N.Thinh, P.G.Dien (2006) Synthesis and Characterization of Chitosan Nanoparticles Used as Drug Carrier Vietnam Journal of Chemistry 44: 105-9 107 T Abdul kareem, A Anu kaliani (2012), Synthesis and thermal study of octahedral silver nano-plates in polyvinyl alcohol (PVA), Arabian Journal of Chemistry, 4, 3, 325-331 108 Vallerie H DeLeon, Thanh D Nguyen, Mangesh Nar, Nandika A D'Souzab, Teresa D Goldena (2012), Polymer nanocomposites for improved drug delivery efficiency, Materials Chemistry andPhysics,132, 2-3, 409-415 109 Vipin Bansal, Pramod Kumar Sharma, Nitin Sharma, Om Prakash Pal and Rishabha Malviy (2011), Applications of Chitosan and Chitosan Derivatives in Drug Delivery, Advances in Biological Research, (1): 28-37 110 Xiaole Zhang, Hongyun Niu, Jinping Yan, Yaqi Cai, (2011), Immobilizing silver nanoparticles onto the surface of magnetic silica composite to prepare magnetic disinfectant with enhanced stability and antibacterial activity Colloids and Surfaces A: Physicochemical and Engineering Aspects, 375(1-3), 186-192 111 Xueping Zhang, Wanquan Jiang, Xinglong Gong, Zhong Zhan, 2010), Sonochemical synthesis and characterization of magnetic separable Fe 3O4/Ag composites and its catalytic properties, Journal of Alloys and Compounds, 508, 400405 112 Wanquan Jiang Wanquan Jiang, Yufeng Zhou, Yanli Zhang, Shouhu Xuan, Xinglong Gong (2012), Superparamagnetic Ag@Fe 3O4 core-shell nanospheres: fabrication, characterization and application as reusable nanocatalyst, Dalton Trans., 41, 4594-4601 113 Wong KK, Cheung SO, Huang L, Niu J, Tao C, Ho CM, Che CM, Tam PK, (2009), Further evidence of the anti-inflammatory effects of silver nanoparticles, ChemMedChem.; 4(7):1129-35 114 Zhang, H.W.; Cai, G.Q.; Tang, G.P.; Wang, L.Q.; Jiang, H.L (2011), Synthesis, self-assembly, and cytotoxicity of well-defined trimethylated chitosan-O-poly(epsiloncaprolactone): Effect of chitosan molecular weight J Biomed Mater Res B, 98B, 290-299 115 Zhang W, Gai L, Li Z, Jiang H and Ma W, (2008), Low temperature hydrothermal synthesis of octahedral Fe3O4 microcrystals, J.Phys D: Appl Phys 41, 225001 116 Zhi Yong Bao, Jiyan Dai, Dang Yuan Lei, and Yucheng W, (2013), Maximizing surface-enhanced Raman scattering sensitivity of surfactantfree Ag-Fe 3O4 nanocomposites through optimization of silver nanoparticle density and magnetic selfassembly, J Appl Phys 114, 124305 117 Ziba Roveimiab, Ali Reza Mahdavian, Esmaeil Biazar, (2012), Preparation of magnetic chitosan nanocompozit: Particles and Their Susceptibility for Cellular Separation Applications, Journal of Colloid Science and Biotechnology; 1:1-7 118 Zohre Zarnegara, Javad Safari (2014), Fe 3O4@chitosan nanoparticles: a valuable heterogeneous nanocatalyst for the synthesis of 2,4,5-trisubstituted imidazoles, RSC Advances, 4, 20932-20939 119 Zong minh Xiu, Quing Bo Zhang, Hema L.Puppala, Vicki l.Colvin, Pedro J.J.Alvarez, (2012), Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles, Nano Lett., 12 (8), 4271-4275 120 Yan Weia, Bing Hanb, Xiaoyang Hua, Yuanhua Linc, Xinzhi Wang, Xuliang Denga, (2012), Synthesis of Fe3O4 Nanoparticles and their Magnetic Properties, Procedia Engineering, 27, 632-637 121 Ying Zhou, Yinghui Zhao, Lu Wang, Ling Xu, Maolin Zhai, Shicheng Wei, (2012), Radiation synthesis and characterization of nanosilver/gelatin/carboxymethyl chitosan hydrogel, Radiation Physics and Chemistry, 81, 5, 553-560 122 Yuan-Yuan Liang and Li-Ming Zhang, (2007), Bioconjugation of Papain on Superparamagnetic Nanoparticles Decorated with Carboxymethylated Chitosan, Biomacromolecules, (5), 1480-1486 114 1.531 1.532 ... CÔNG NGHỆ NGHIÊN CỨU, TỔNG HỢP, ĐẶC TRƯNG VẬT LIỆU LAI VÔ CƠ (Ag, Fe3O4) - HỮU CƠ (CHITOSAN) CẤU TRÚC NANO ĐỊNH HƯỚNG ỨNG DỤNG TRONG Y SINH LUẬN ÁN TIẾN SỸ HÓA HỌC Chuyên ngành: Vật liệu cao phân... Ngoài ứng dụng vượt trội vật liệu polyme nanocompozit lĩnh vực x y dựng, điện tử, gia dụng tính chất lý trội vật liệu nanocompozit tăng độ bền, chịu nhiệt, giảm độ thấm khí Ng y nay, vật liệu nanocompozit... 87] 1.37 Vật liệu polyme nanocompozit, kết kết hợp polyme vật liệu nano, thể ưu điểm thành phần chất gia cường nano pha phân tán polyme Lý thuyết, mô hình chế tạo ứng dụng loại vật liệu quan