Nghiên cứu quy trình giám định một số chất ma túy tổng hợp nhóm ATS trong nước tiểu bằng phương pháp điện di mao quản

I HC QUC GIA H NI TRNG I HC KHOA HC T NHIấN - Lấ TH THANH XUN NGHIấN CU QUY TRèNH GIM NH MT S CHT MA TY TNG HP NHểM ATS TRONG NC TIU BNG PHNG PHP IN DI MAO QUN LUN VN THC S KHOA HC H Ni - 2014 I HC QUC GIA H NI TRNG I HC KHOA HC T NHIấN - Lấ TH THANH XUN NGHIấN CU QUY TRèNH GIM NH MT S CHT MA TY TNG HP NHểM ATS TRONG NC TIU BNG PHNG PHP IN DI MAO QUN Chuyờn ngnh: Húa phõn tớch Mó s: 60440118 LUN VN THC S KHOA HC NGI HNG DN KHOA HC - HD 1: TS Nguyn Xuõn Trng - HD 2: TS Nguyn Th nh Hng H Ni - 2014 LI CM N Tụi xin by t lũng bit n sõu sc v chõn thnh nht n vi TS Nguyn Xuõn Trng v TS Nguyn Th nh Hng Thy, cụ ó giao ti, nhit tỡnh hng dn v to mi iu kin thun li nht giỳp tụi thc hin lun ny Tụi xin chõn thnh cm n GS Peter C Hauser, TS Mai Thanh c v ThS Bựi Duy Anh ó h tr trang thit b cho nghiờn cu ny Tụi xin chõn thnh cm n cỏc thy, cụ b mụn Húa Phõn tớch núi riờng v khoa Húa hc núi chung ó dy d, ch bo v ng viờn tụi thi gian tụi hc ti trng i hc Khoa hc T nhiờn H Ni Tụi chõn thnh cm n cỏc cỏn b giỏm nh ma tỳy ca Trung tõm Giỏm nh ma tỳy, Vin Khoa hc Hỡnh s, B Cụng an ó to mi iu kin v giỳp tụi quỏ trỡnh x lý mu v thc hin phõn tớch i chng Qua õy, tụi xin cm n gia ỡnh, cỏc bn hc viờn v sinh viờn ca B mụn Húa phõn tớch ó luụn ng viờn, tn tỡnh giỳp tụi thi gian hc v thc hin lun ny H Ni, ngy 21 thỏng 12 nm 2014 Hc viờn Lờ Th Thanh Xuõn DANH MC CC CH VIT TT Tờn vit tt Tờn y Ace Axetic Arg L- arginin C4D Detector o dn kt ni kiu t in CE Phng phỏp in di mao qun EOF Dũng in di thm thu GC Sc ký khớ GC/MS Sc ký khớ ph His Histidin HPLC Sc ký lng hiu nng cao Leff Chiu di hiu dng ca mao qun Ltot Tng chiu di mao qun LOD Gii hn phỏt hin LOQ Gii hn nh lng MEKC in di mao qun in ng hc Mixen MA Methamphetamin.HCl MDA Methylenedioxyamphetamin.HCl MDMA Methylenedioxymethamphetamin MDEA 3,4-Methylenedioxyethamphetamin.HCl M Mu Phos Photphoric Ppm Parts per million: phn triu %RSD % lch chun tng i SD lch chun UPLC Sc ký lng siờu hiu nng ATS Amphetamin type stimulants MC LC M U Error! Bookmark not defined CHNG 1: TNG QUAN Error! Bookmark not defined 1.1 Gii thiu chung v cht ma tỳy Error! Bookmark not defined 1.1.1 Mt s khỏi nim v cht ma tỳy Error! Bookmark not defined 1.1.2 Phõn loi cỏc cht ma tỳy Error! Bookmark not defined 1.1.3 Cỏc cht ma tỳy tng hp nhúm ATS Error! Bookmark not defined 1.1.3.1 Thụng tin chung v ma tỳy tng hp nhúm ATS Error! Bookmark not defined 1.1.3.2 Thụng tin chung v bn cht ma tỳy tng hp c phõn tớch Error! Bookmark not defined 1.1.3.3 Ngun gc, tng hp ca MA, MDA, MDMA, MDEA Error! Bookmark not defined 1.1.3.4 Tỏc dng i vi c th v c ch tỏc dng ca nhúm ATS Error! Bookmark not defined 1.1.3.5 Hp thu, phõn b, chuyn húa v thi tr Error! Bookmark not defined 1.2 Vn s dng ma tỳy tng hp nhúm ATS trờn th gii v Vit Nam Error! Bookmark not defined 1.2.1 Vn s dng ma tỳy tng hp nhúm ATS trờn th gii Error! Bookmark not defined 1.2.2 Vn s dng ma tỳy tng hp nhúm ATS Vit Nam Error! Bookmark not defined 1.3 Cỏc phng phỏp x lý mu phm sinh hc cha ma tỳy tng hp nhúm ATS Error! Bookmark not defined 1.3.1 Chit lng - lng Error! Bookmark not defined 1.3.2 Chit pha rn Error! Bookmark not defined 1.4 Cỏc phng phỏp xỏc nh ma tỳy tng hp nhúm ATS Error! Bookmark not defined 1.4.1 Phng phỏp phõn tớch dch hc (immunoassay) Error! Bookmark not defined 1.4.2 Cỏc phng phỏp sc kớ Error! Bookmark not defined 1.4.2.1 Phng phỏp sc ký lng Error! Bookmark not defined 1.4.2.2 Phng phỏp sc ký khớ - ph (GC/MS) Error! Bookmark not defined 1.4.2.3 Phng phỏp in di mao qun Error! Bookmark not defined 1.5 Gii thiu chung v phng phỏp in di mao qun (CE) Error! Bookmark not defined 1.5.1 Mao qun Error! Bookmark not defined 1.5.2 Dung dch m, pH v pha ng phng phỏp in di mao qun Error! Bookmark not defined 1.5.3 Ngun in th cao Error! Bookmark not defined 1.5.4 Cỏc k thut bm mu phng phỏp in di mao qun Error! Bookmark not defined 1.5.5 Detector phng phỏp in di mao qun Error! Bookmark not defined CHNG 2: THC NGHIM Error! Bookmark not defined 2.1 Mc tiờu v ni dung nghiờn cu Error! Bookmark not defined 2.1.1 Mc tiờu nghiờn cu Error! Bookmark not defined 2.1.2 Ni dung nghiờn cu Error! Bookmark not defined 2.2 Phng phỏp nghiờn cu Error! Bookmark not defined 2.2.1 Phng phỏp phõn tớch Error! Bookmark not defined 2.2.2 Phng phỏp x lý mu Error! Bookmark not defined 2.2.2.1 X lý mu viờn thuc Error! Bookmark not defined 2.2.2.2 X lý mu nc tiu Error! Bookmark not defined 2.3 Cỏc thụng s ỏnh giỏ tin cy ca phng phỏp phõn tớch Error! Bookmark not defined 2.3.1 Gii hn phỏt hin (LOD) v gii hn nh lng (LOQ) Error! Bookmark not defined 2.3.2 chm ( lp li) ca phng phỏp Error! Bookmark not defined 2.3.3 ỳng ( thu hi) ca thit b, ca phng phỏp Error! Bookmark not defined 2.4 Thc nghim Error! Bookmark not defined 2.4.1 Húa cht Error! Bookmark not defined 2.4.1.1 Cht chun Error! Bookmark not defined 2.4.1.2 Húa cht dung mụi Error! Bookmark not defined 2.4.1.3 Chun b cỏc dung dch húa cht Error! Bookmark not defined 2.4.2 Thit b dng c Error! Bookmark not defined CHNG 3: KT QU V THO LUN Error! Bookmark not defined 3.1 Nghiờn cu kho sỏt ti u iu kin tỏch MA, MDA, MDMA, MDEA bng phng phỏp in di mao qun CE-C4D Error! Bookmark not defined 3.1.1 Kho sỏt nh hng ca h m Error! Bookmark not defined 3.1.1.1 Kho sỏt thnh phn ca h m in diError! Bookmark not defined 3.1.1.2 Kho sỏt pH ca dung dch m in di Error! Bookmark not defined 3.1.1.3 Kho sỏt nng dung dch m in di Error! Bookmark not defined 3.1.2 Kho sỏt nh hng ca thi gian bm mu Error! Bookmark not defined 3.1.3 Kho sỏt nh hng ca th t vo hai u mao qun Error! Bookmark not defined 3.2 Kho sỏt cỏc cht gõy nh hng Error! Bookmark not defined 3.3 ỏnh giỏ phng phỏp phõn tớch (Thm nh phng phỏp) Error! Bookmark not defined 3.3.1 Lp ng chun Error! Bookmark not defined 3.3.2 Gii hn phỏt hin (LOD) v gii hn nh lng (LOQ) ca thit b Error! Bookmark not defined 3.3.2.1 Gii hn phỏt hin (LOD) Error! Bookmark not defined 3.3.2.2 Gii hn nh lng (LOQ) Error! Bookmark not defined 3.3.3 ỏnh giỏ chm ( lp li) v ỳng ( thu hi) Error! Bookmark not defined 3.3.3.1 chm ca thit b Error! Bookmark not defined 3.3.3.2 ỳng ca phng phỏp Error! Bookmark not defined 3.4 Phõn tớch mu thc t v o i chng vi phng phỏp tiờu chun GC/MS Error! Bookmark not defined 3.4.1 Phõn tớch mu ma tỳy b bt gi Error! Bookmark not defined 3.4.1.1 Mu MA dng viờn Error! Bookmark not defined 3.4.1.2 Mu ma tỳy ỏ cha MA Error! Bookmark not defined 3.4.1.3 Mu ma tỳy cha MDMA dng viờn nộn Error! Bookmark not defined 3.4.1.4 Kt qu phõn tớch mu nc tiu Error! Bookmark not defined 3.4.2 Phõn tớch i chng phng phỏp CE-C4D vi phng phỏp GC/MS Error! Bookmark not defined KT LUN Error! Bookmark not defined TI LIU THAM KHO 12 PH LC Error! Bookmark not defined DANH MC CC BNG Bng 1.1 Thụng tin chung v cht ma tỳy tng hp nhúm ATS: MA, MDA, MDMA, MDEA [2] Error! Bookmark not defined Bng 1.2 Cỏc cht thng dựng lm pha ng CE Error! Bookmark not defined Bng 3.1 S ph thuc din tớch pic ca MA, MDA, MDMA v MDEA vo thnh phn h m in di Error! Bookmark not defined Bng 3.2 Kt qu kho sỏt nh hng ca pH n din tớch pic (Spic) v thi gian di chuyn (tdc) ca MA, MDA, MDMA, MDEA Error! Bookmark not defined Bng 3.3 Kt qu kho sỏt s ph thuc ca din tớch pic (Spic) v thi gian di chuyn (tdc) ca MA, MDA, MDMA, MDEA vo nng dung dch m in di Error! Bookmark not defined Bng 3.4 Kt qu s ph thuc din tớch pic (Spic) v thi gian di chuyn (tdc) ca MA, MDA, MDMA, MDEA vo thi gian bm mu Error! Bookmark not defined Bng 3.5 nh hng ca in th tỏch (E) n thi gian di chuyn ca cỏc cht phõn tớch Error! Bookmark not defined Bng 3.6 iu kin ti u cho phõn tớch hn hp MA, MDA, MDMA, MDEA bng phng phỏp CE-C4D Error! Bookmark not defined Bng 3.7 S ph thuc ca din tớch pic vo nng MA, MDA, MDMA, MDEA t=15 s Error! Bookmark not defined Bng 3.8 Phng trỡnh ng chun ca MA, MDA, MDMA, MDEA t=15 s Error! Bookmark not defined Bng 3.9 Kt qu so sỏnh gia giỏ tr a vi giỏ tr ca phng trỡnh ng chun MA, MDA, MDMA, MDEA t = 15 s Error! Bookmark not defined Bng 3.10 S ph thuc ca din tớch pic trung bỡnh vo nng ca MA, MDA, MDMA v MDEA t = 45 s Error! Bookmark not defined Bng 3.11 Phng trỡnh ng chun ca MA, MDA, MDMA, MDEA t=45 s Error! Bookmark not defined Bng 3.12 Kt qu so sỏnh gia giỏ tr a vi giỏ tr ca phng trỡnh ng chun MA, MDMA, MDMA, MDEA t= 45 s Error! Bookmark not defined Bng 3.13 Tớnh gii hn phỏt hin ca MA, MDA, MDMA, MDEA t =15 s Error! Bookmark not defined Bng 3.14 Tớnh gii hn phỏt hin ca MA, MDA, MDMA, MDEA t=45 s Error! Bookmark not defined Bng 3.15 Giỏ tr khong tuyn tớnh v LOD, LOQ ca MA, MDA, MDMA, MDEA t =15 s Error! Bookmark not defined Bng 3.16 Giỏ tr khong tuyn tớnh v LOD, LOQ ca MA, MDA, MDMA, MDEA t = 45 s Error! Bookmark not defined Bng 3.17 Kt qu xỏc nh lp li ca phng phỏp CE - C4D nh lng MA Error! Bookmark not defined Bng 3.18 Kt qu xỏc nh lp li ca phng phỏp CE - C4D nh lng MDA Error! Bookmark not defined Bng 3.19 Kt qu xỏc nh lp li ca phng phỏp CE - C4 D nh lng MDMA Error! Bookmark not defined Bng 3.20 Kt qu xỏc nh lp li ca phng phỏp CE-C4D nh lng MDEA Error! Bookmark not defined Bng 3.21 Kt qu tớnh gii hn phỏt hin (LOD), gii hn nh lng (LOQ) ca phng phỏp i vi MA, MDA, MDMA, MDEA Error! Bookmark not defined Bng 3.22 Kt qu kho sỏt ỳng ca phng phỏp da trờn thờm chun MA Error! Bookmark not defined Bng 3.23 Kt qu kho sỏt ỳng ca phng phỏp da trờn thờm chun MDA Error! Bookmark not defined Bng 3.24 Kt qu kho sỏt ỳng ca phng phỏp da trờn thờm chun MDMA Error! Bookmark not defined Bng 3.25 Kt qu kho sỏt ỳng ca phng phỏp da trờn thờm chun MDEA Error! Bookmark not defined Bng 3.26 Thụng tin v c im ca tng loi mu ma tỳy b bt gi Error! Bookmark not defined Bng 3.27 Kt qu xỏc nh hm lng MA mu viờn nộn cha MA Error! Bookmark not defined Bng 3.28 Kt qu xỏc nh nng MA mu MA ỏ bng phng phỏp thờm chun Error! Bookmark not defined Bng 3.29 Kt qu xỏc nh nng MA mu 66 Error! Bookmark not defined Bng 3.30 Kt qu phõn tớch 12 mu nc tiu dng tớnh bng CE Error! Bookmark not defined Bng 3.31 Kt qu phõn tớch i chng 12 mu nc tiu vi phng phỏp tiờu chun GC/MS Error! Bookmark not defined DANH MC CC HèNH Hỡnh 1.1 Qui trỡnh chit xut ma tỳy tng hp nhúm ATS nc tiu Error! Bookmark not defined Hỡnh 1.2 Mụ hỡnh cu to ca mt h phõn tớch in di mao qun Error! Bookmark not defined Hỡnh 1.3 Mt ct ngang ca mao qun Error! Bookmark not defined Hỡnh 1.4 Lp in tớch kộp trờn b mt mao qun Error! Bookmark not defined Hỡnh 1.5 nh hng ca dũng EOF n tc cỏc ion quỏ trỡnh in di Error! Bookmark not defined Hỡnh 1.6 Cỏc k thut bm mu phng phỏp in di mao qun Error! Bookmark not defined Hỡnh 1.7 Nguyờn lý hot ng ca cm bin o dn khụng tip xỳc Error! Bookmark not defined Hỡnh 1.8 Quỏ trỡnh chuyn i tớn hiu ca C4D Error! Bookmark not defined Hỡnh 2.1 H in di s dng detector o dn khụng tip xỳc Error! Bookmark not defined Hỡnh 3.1 in di kho sỏt s nh hng ca thnh phn h m n s phõn tỏch ca MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.2 S ph thuc din tớch pic ca MA, MDA, MDMA, MDEA vo thnh phn h m in di Error! Bookmark not defined Hỡnh 3.3 in di kho sỏt nh hng ca pH n s phõn tỏch ca MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.4 th th hin mi tng quan gia tớn hiu din tớch pic ca MDA v phõn gii R ca pic MDA v MDMA ph thuc vo pH ca dung dch m in di Error! Bookmark not defined Hỡnh 3.5 in di kho sỏt nh hng ca nng dung dch m in di n quỏ trỡnh phõn tỏch cỏc cht MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.6 in di kho sỏt s nh hng ca thi gian bm mu Error! Bookmark not defined Hỡnh 3.7 in di kho sỏt s nh hng ca in th tỏch n thi gian di chuyn v s phõn tỏch cỏc pic Error! Bookmark not defined Hỡnh 3.8 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn MA ỏ Error! Bookmark not defined Hỡnh 3.9 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn MA viờn Error! Bookmark not defined Hỡnh 3.10 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn mu nc tiu s 17 Error! Bookmark not defined Hỡnh 3.11 ng chun ca MA theo din tớch pic t = 15 s Error! Bookmark not defined Hỡnh 3.12 ng chun ca MDA theo din tớch pic t = 15 s Error! Bookmark not defined Hỡnh 3.13 ng chun ca MDMA theo din tớch pic t = 15 s Error! Bookmark not defined Hỡnh 3.14 ng chun ca MDEA theo din tớch pic t = 15 s Error! Bookmark not defined Hỡnh 3.15 ng chun ca MA theo din tớch pic t = 45 s Error! Bookmark not defined Hỡnh 3.16 ng chun ca MDMA theo din tớch pic t = 45 s Error! Bookmark not defined Hỡnh 3.17 ng chun ca MDA theo din tớch pic t = 45 sError! Bookmark not defined Hỡnh 3.18 ng chun ca MDEA theo din tớch pic t = 45 s Error! Bookmark not defined Hỡnh 3.19 in di xỏc nh MA viờn nộn sau thờm chun MA cỏc nng khỏc Error! Bookmark not defined Hỡnh 3.20 in di xỏc nh ng thi MA, MDA, MDMA, MDEA nn mu viờn nộn cha MA Error! Bookmark not defined Hỡnh 3.21 in di xỏc nh MA mu ma tỳy ỏ cha MA sau thờm chun MA nng khỏc Error! Bookmark not defined Hỡnh 3.22 in di xỏc nh s cú mt ng thi ca MA, MDA, MDMA, MDEA nn mu MA ỏ Error! Bookmark not defined Hỡnh 3.23 in di xỏc nh MDMA viờn nộn Error! Bookmark not defined Hỡnh 3.24 in di xỏc nh s cú mt ca MA mu 66 bng cỏch thờm chun MA nng khỏc Error! Bookmark not defined Hỡnh 3.25 in di xỏc nh s cú mt ca MDMA mu nc tiu s bng cỏch thờm chun MDMA nng khỏc Error! Bookmark not defined Hỡnh 3.26 in di xỏc nh s cú mt ca MDMA mu nc tiu s bng cỏch thờm chun MDMA nng khỏc Error! Bookmark not defined Hỡnh 3.27 in di xỏc nh s cú mt ca MDMA mu nc tiu s bng cỏch thờm chun MDMA nng khỏc Error! Bookmark not defined TI LIU THAM KHO TING VIT Mai Thanh c, Nguyn Th nh Hng (2013), in di mao qun kt ni vi cm bin o dn khụng tip xỳc CE-C4D, Ti liu lu hnh ni b Trn Minh Hng (2004), Cỏc cht ma tỳy thng gp v phng phỏp giỏm nh mu phm sinh hc, Nh xut bn cụng an nhõn dõn Nguyn Th nh Hng (2010), Nghiờn cu xỏc nh cỏc dng asen vụ c nc ngm bng phng phỏp in di mao qun s dng detector dn khụng tip xỳc, Lun ỏn Tin s Húa hc, Trng i hc Khoa hc T nhiờn - HQGHN ng c khanh - Vin Phỏp y Quõn i;Trn Vit Hựng-Vin Kim nghim thuc Trung ng; Trn Th Thỳy- Trng i hc Bỏch khoa H Ni (2011), Xõy dng quy trỡnh phõn tớch ng thi cỏc cht ma tỳy tng hp MA, MDA, MDMA nc tiu bng phng phỏp sc ký khớ ph, Bỏo quõn i nhõn dõn online, Fax: (84 - 4) 3747- 4913 Liờn hp quc (2014), Cụng b Bỏo cỏo tỡnh hỡnh ma tỳy th gii nm 2014do cỏc t chc Liờn hp quc ti Vit Nam t chc ngy 26/6 ti H Ni, Cng thụng tin in t B lao ng thng binh v xó hi Phm Lun (2005), C s lý thuyt ca Sc kớ in di mao qun hiu nng cao, Giỏo trỡnh ging dy dnh cho sinh viờn chuyờn ngnh Húa Phõn tớch, Trng H Khoa hc T Nhiờn H Ni Quc hi nc Cng hũa xó hi ch ngha Vit Nam khúa X, k hp th thụng qua ngy 09 thỏng 12 nm 2000 Lut s 23/2000/QH10 ca Quc hi: Lut Phũng, Chng ma tỳy Nguyn Vn Ri (2011), Cỏc k thut phõn tớch in di, Sỏch chuyờn cao hc, i hc khoa hc T nhiờn - HQG H Ni Nguyn Vn Ri (2011), Cỏc phng phỏp tỏch sc ký, Sỏch chuyờn cao hc, i hc khoa hc T nhiờn-HQG H Ni 10 T Th Tho (2010), Bi ging chuyờn thng kờ húa phõn tớch, H Quc gia H Ni 11 Th tng Chớnh ph ban hnh, Quyt nh 1203/Q-TTg phờ duyt chng trỡnh mc tiờu quc gia phũng, chng ma tỳy giai on 2012 - 2015 12 Th tng Chớnh ph, Quyt nh s 93/2001/Q-TTG: V thỏng hnh ng phũng, chng ma tỳy v ngy ton dõn phũng, chng ma tỳy 13 Vin kim nghim ATVSTP Quc Gia (2010), Thm nh phng phỏp phõn tớch húa hc v vi sinh vt, Nh xut bn khoa hc k thut H Ni TING ANH 14 Aysel eztunc, Armagan enal, et al (2012), Detection of Methamphetamine, Methylenedioxymethamphetamine,3,4-Methylenedioxy-Nethylamphetamine in Spiked Plasma by HPLC and TLC, Journal of AOAC International, vol 93, N02 15 Alison Beavis Claude Roux Philip Doble, (2014), The use of organic and inorganic impurities found in MDMA police seizures in a drug intelligence perspective, Centre for Forensic Science, Department of Chemistry and Forensic Science, University of Technology Sydney (UTS), Sydney, Australia 16 Alison Beavis Claude Roux, Philip Doble (2010), Analysis of amphetaminetype substances by capillary zone electrophoresis using capacitively coupled contactless conductivity detection, Strategy forum japan 2014,pp 2608-2613 17 Bruno S, De Martinis A, Allan J, Barnes B, Karl B, Scheidweiler B, Marilyn A Huestis (2007), Development and validation of a disk solid phase extraction and gas chromatography-mass spectrometry method for MDMA, MDA, HMMA, HMA, MDEA, methamphetamine and amphetamine in sweat, Journal of Chromatography B, 852, pp.450-458 18 B.K Gan, D Baugh, R.H Liu and A.S Walia (1991), Simultaneous analysis of ampheta- mine, methamphetamine, and 3,4-methylen dioxy metham phetamin in urine samples by solid-phase extraction, derivatisation, and gas chromatography/mass spectromctry, J Forens Sci 36, pp 1331- 1341 19 D.Schlegel, J Mattusch, R Wennrich (1996), Speciation analysis of arsenic and selenium compounds by capillary electrophoresis, Fres J Anal Chem (354), pp 535-539 20 da Costa JL, da Matta Chasin AA.(2004), Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection J Chromatogr B Analyt Technol Biomed Life Sci , (811), pp 41-45 21 Hajime Miyaguchi, Hiroyuki Inoue (2011), Determination of amphetaminetype stimulants, cocaine and ketamine in human hair by liquid chromatography/linear ion trap-orbitrap hybrid mass spectrometry, Analyst, 136, pp 3503 22 Laurian Vlase, Daniela-Saveta Popa, Felicia Loghin, Sorin E (2009), LeucutaHigh-throughput toxicological analysis of Methamphetamine, MDA and MDMA from human plasma by LC-MS/MS, Romanian Society of Legal Medicine ,3, pp 213 - 220 23 Moffat, A C., Osselton, M D., Widdop, B., Galichet, L Y (Eds.) (1994) Clarkes analysis of drugs and poisons, Pharma- ceutical Press, London 2004 Pickering, G V S., Addiction, 89, pp 1385 24 Pavel Kubá, Peter C Hauser (2008), A review of the recent achievements in capacitively coupled contactless conductivity detection, Analytica Chimica Acta (607), pp 15-29 25 Rochelle Epple, Lucas Blanes, Alison Beavis, Claude Roux, Philip Doble (2010), Analysis of amphetamine-type substances by capillary zone electrophoresis using capacitively coupled contactless conductivity detection, Electrophoresis, 31, pp 2608-2613 26 Ragnhild Elộn Gjulem Jamt, Astrid Gjelstad, Lars Erik Eng Eibak , Elisabeth Leere ỉiestad, Asbjứrg Solberg Christophersen, Knut Einar Rasmussen, Stig Pedersen-Bjergaard stimulating drugs from (2012), Electromembrane undiluted whole blood, extraction of Journal of Chromatography A, 1232 , pp 27-36 27 Satoshi Chinaka, Reiko Iio, Nariaki Takayama, Shuji Kodama, and Kazuichi Hayakawa (2006), Chiral Capillary Electrophoresis of Amphetamine-Type Stimulants, Journal of Health Science, 52(6), pp 649-654 28 Thitirat Mantim, Duangjai Nacapricha, Prapin Wilairat, Peter C Hauser (2012), Enantiomeric separation of some common ontrolledstimulants by capillary electrophoresis with contactless conductivity detection, Electrophoresis 2012, 33, pp 388-394 29 Worapan Pormsila, Rộjane Morand, Stephan Krọhenbỹhl, Peter C Hauser (2011), Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples, Journal of Chromatography B, 879 (2011), pp 921-926 30 United nations international drug control programme (2011), A summary of commercially available products and their applications: guidance for the selection of suitable product, Scientific and technical notes, pp 5-6 31 United nations office on drugs and crime (2010), World drug report 2010, United nations, New York, pp 95-96 [...]... MDMA, MDEA trong nn mu viờn nộn cha MA Error! Bookmark not defined Hỡnh 3.21 in di xỏc nh MA trong mu ma tỳy ỏ cha MA sau khi thờm chun MA 3 nng khỏc nhau Error! Bookmark not defined Hỡnh 3.22 in di xỏc nh s cú mt ng thi ca MA, MDA, MDMA, MDEA trong nn mu MA ỏ Error! Bookmark not defined Hỡnh 3.23 in di xỏc nh MDMA trong viờn nộn Error! Bookmark not defined Hỡnh 3.24 in di xỏc nh... nh s cú mt ca MA trong mu 66 bng cỏch thờm chun MA 3 nng khỏc nhau Error! Bookmark not defined Hỡnh 3.25 in di xỏc nh s cú mt ca MDMA trong mu nc tiu s 2 bng cỏch thờm chun MDMA 1 nng khỏc Error! Bookmark not defined Hỡnh 3.26 in di xỏc nh s cú mt ca MDMA trong mu nc tiu s 5 bng cỏch thờm chun MDMA 1 nng khỏc Error! Bookmark not defined Hỡnh 3.27 in di xỏc nh s cú mt ca MDMA trong mu nc tiu... Bookmark not defined Hỡnh 3.1 in di kho sỏt s nh hng ca thnh phn h m n s phõn tỏch ca MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.2 S ph thuc din tớch pic ca MA, MDA, MDMA, MDEA vo thnh phn h m in di Error! Bookmark not defined Hỡnh 3.3 in di kho sỏt nh hng ca pH n s phõn tỏch ca MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.4 th th hin mi tng quan gia tớn hiu din... ca MDMA theo din tớch pic khi t = 45 s Error! Bookmark not defined Hỡnh 3.17 ng chun ca MDA theo din tớch pic khi t = 45 sError! Bookmark not defined Hỡnh 3.18 ng chun ca MDEA theo din tớch pic khi t = 45 s Error! Bookmark not defined Hỡnh 3.19 in di xỏc nh MA trong viờn nộn sau khi thờm chun MA cỏc nng khỏc nhau Error! Bookmark not defined Hỡnh 3.20 in di xỏc nh ng thi MA, MDA, MDMA,... trờn b mt mao qun Error! Bookmark not defined Hỡnh 1.5 nh hng ca dũng EOF n tc cỏc ion trong quỏ trỡnh in di Error! Bookmark not defined Hỡnh 1.6 Cỏc k thut bm mu trong phng phỏp in di mao qun Error! Bookmark not defined Hỡnh 1.7 Nguyờn lý hot ng ca cm bin o dn khụng tip xỳc Error! Bookmark not defined Hỡnh 1.8 Quỏ trỡnh chuyn i tớn hiu ca C4D Error! Bookmark not defined Hỡnh 2.1 H in di s dng... chun MDEA Error! Bookmark not defined Bng 3.26 Thụng tin v c im ca tng loi mu ma tỳy b bt gi Error! Bookmark not defined Bng 3.27 Kt qu xỏc nh hm lng MA trong mu viờn nộn cha MA Error! Bookmark not defined Bng 3.28 Kt qu xỏc nh nng MA trong mu MA ỏ bng phng phỏp thờm chun Error! Bookmark not defined Bng 3.29 Kt qu xỏc nh nng MA trong mu 66 Error! Bookmark not defined Bng 3.30 Kt... defined Hỡnh 3.8 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn MA ỏ Error! Bookmark not defined Hỡnh 3.9 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn MA viờn Error! Bookmark not defined Hỡnh 3.10 in di kho sỏt s nh hng ca cỏc cation n s phõn tỏch ca MA, MDA, MDMA, MDEA trờn nn mu nc tiu s 17 Error! Bookmark not defined Hỡnh... v MDMA ph thuc vo pH ca dung dch m in di Error! Bookmark not defined Hỡnh 3.5 in di kho sỏt nh hng ca nng dung dch m in di n quỏ trỡnh phõn tỏch cỏc cht MA, MDA, MDMA, MDEA Error! Bookmark not defined Hỡnh 3.6 in di kho sỏt s nh hng ca thi gian bm mu Error! Bookmark not defined Hỡnh 3.7 in di kho sỏt s nh hng ca in th tỏch n thi gian di chuyn v s phõn tỏch cỏc pic Error! Bookmark... bng CE Error! Bookmark not defined Bng 3.31 Kt qu phõn tớch i chng 12 mu nc tiu vi phng phỏp tiờu chun GC/MS Error! Bookmark not defined DANH MC CC HèNH Hỡnh 1.1 Qui trỡnh chit xut ma tỳy tng hp nhúm ATS trong nc tiu Error! Bookmark not defined Hỡnh 1.2 Mụ hỡnh cu to ca mt h phõn tớch in di mao qun Error! Bookmark not defined Hỡnh 1.3 Mt ct ngang ca mao qun Error! Bookmark not defined Hỡnh... chun ca MA theo din tớch pic khi t = 15 s Error! Bookmark not defined Hỡnh 3.12 ng chun ca MDA theo din tớch pic khi t = 15 s Error! Bookmark not defined Hỡnh 3.13 ng chun ca MDMA theo din tớch pic khi t = 15 s Error! Bookmark not defined Hỡnh 3.14 ng chun ca MDEA theo din tớch pic khi t = 15 s Error! Bookmark not defined Hỡnh 3.15 ng chun ca MA theo din tớch pic khi t = 45 s Error! Bookmark not