2.1 Introduction························································ 39
2.2 Materials··························································· 40
2.3 Methods··························································· 40
2.3.2 Determination of Particle Size································· 41
2.3.3 Determination of Zeta Potential································ 41
2.3.5 Shaking Test·················································· 42
2.3.6 Statistical Analysis············································ 43
2.4.3 Effect of Secondary Emulsifier on the Properties of Emulsion··· 46
3.1 Introduction ······················································· 57
3.2 Materials··························································· 58
3.3.7 Interaction of Tributyrin Emulsion with LDL and HDL·········· 65
3.3.7.2 Electron Micrographs of Emulsion-LDL Complex········· 66
3.4.2 Characterization of Tributyrin Emulsion and Lipoproteins······· 72
3.4.3 Interaction of Tributyrin Emulsion with LDL and HDL·········· 73
3.4.4 Electron Micrographs of Emulsion-LDL Complex ············· 76
3.5 Discussion························································· 80
4.1 Introduction························································ 84
4.2 Materials··························································· 85
4.3 Methods··························································· 85
5.1 Introduction························································ 99
5.2 Materials and Methods·············································· 100
5.3 Results····························································· 104
5.4 Discussion ························································· 109
6.1 Introduction························································ 114
CHAPTER ONE
1.1 Butyric Acid from Diet
CHAPTER TWO
2.1 Introduction
2.2 Materials
2.3 Methods
2.3.2 Determination of Particle Size
2.3.3 Determination of Zeta Potential
2.3.5 Shaking Test
2.3.6 Statistical Analysis
2.4.3 Effect of Secondary Emulsifier on the Properties of Emulsion
CHAPTER THREE
3.1 Introduction
3.2 Materials
3.3.7 Interaction of Tributyrin Emulsion with LDL and HDL
3.3.7.2 Electron Micrographs of Emulsion-LDL Complex
3.4.2 Characterization of Tributyrin Emulsion and Lipoproteins
3.4.3 Interaction of Tributyrin Emulsion with LDL and HDL
3.4.4 Electron Micrographs of Emulsion-LDL Complex
3.5 Discussion
CHAPTER FOUR
4.1 Introduction
4.2 Materials
4.3 Methods
CHAPTER FIVE
5.1 Introduction
5.2 Materials and Methods
5.3 Results
5.4 Discussion
CHAPTER SIX
6.1 Introduction
CHAPTER SEVEN
7.1 Introduction
7.2 Materials and Methods
7.2.4 Interaction of ATRA Emulsion with Lipoprotein Fractions
CHAPTER EIGHT
SDS Polyacrylamide Gel Electrophoresis
(SDS-PAGE)