ProfessorB.Austin SchoolofLifeSciences JohnMuirBuilding HeriotWattUniversity Riccarton Edinburgh UK DrD.A.Austin ResearchAssociate HeriotWattUniversity Riccarton Edinburgh UK SPRINGERPRAXISBOOKSINAQUATICANDMARINESCIENCES SUBJECTADVISORYEDITOR:DrPeterDobbinsPh.D.,CEng.,F.I.O.A.,SeniorConsultant,MarineDevision, SEA,Bristol,UK ISBN9781402060687SpringerDordrechtBerlinHeidelbergNewYork SpringerispartofSpringerScience+BusinessMedia(springer.com) AcataloguerecordofthisbookisavailablefromtheLibraryofCongress Apartfromanyfairdealingforthepurposesofresearchorprivatestudy,orcriticism orreview,aspermittedundertheCopyright,DesignsandPatentsAct1988,this publicationmayonlybereproduced,storedortransmitted,inanyformorbyany means,withthepriorpermissioninwritingofthepublishers,orinthecaseof reprographicreproductioninaccordancewiththetermsoflicencesissuedbythe CopyrightLicensingAgency.Enquiriesconcerningreproductionoutsidethoseterms shouldbesenttothepublishers. ©PraxisPublishingLtd,Chichester,UK,2007 PrintedinGermany Theuseofgeneraldescriptivenames,registerednames,trademarks,etc.inthis publicationdoesnotimply,evenintheabsenceofaspecificstatement,thatsuch namesareexemptfromtherelevantprotectivelawsandregulationsandthereforefree forgeneraluse. Coverdesign:JimWilkie Projectmanagement:OriginatorPublishingServicesLtd,GtYarmouth,Norfolk,UK Printedonacidfreepaper
[...]... bacterial fish pathogens Diagnostic traits of the Gram-negative bacterial fish pathogens Experimental data concerning the survival of A salmonicida in water Methods of controlling bacterial fish diseases Composition of the purified basal medium to which different concentrations of vitamin C at 0-150mg/kg were added Vaccines for A salmonicida Methods for appHcation of antimicrobial compounds to fish Methods... recovered from moribund and dead rainbow trout fry Methods of isolation for bacterial fish pathogens External signs of disease associated with the bacterial fish pathogens Internal signs of disease Profiles of fish pathogens obtained with the API 20E rapid identification system Differential characteristics of some fish pathogens obtained with the API 20NE rapid identification system Distinguishing profiles... representative Pseudomonadaceae representatives Vibrionaceae representatives Miscellaneous pathogens "Candidatus Arthromitus" Unknown Gram-negative rod 10 Control Wild fish stocks Farmed fish Husbandry Genetically resistant stock Adequate diets/dietary supplements Vaccines Composition of bacterial fish vaccines Methods of vaccine inactivation Methods of administering vaccines to fish Vaccine development... than wild, fish The scope of the book covers all of the bacterial taxa that have at one time or another been reported as fish pathogens Of course, it is reahsed that some taxa are merely secondary invaders of already damaged tissues, whereas others comprise serious, primary pathogens Shortcomings in the literature or gaps in the overall understanding of the subject have been highlighted xvi Preface... Serratia xxv xxvi Abbreviations and acronyms SKDM SSH Sta Sir TCBS TCID TSA TSB V Vag VAM vapA VHH VRML Y Selective Kidney Disease Medium Suppression Subtractive Hybridisation Staphylococcus Streptococcus Thiosulphate Citrate Bile Salts Sucrose Agar Tissue Culture Infectivity Dose Tryptone Soya Agar Tryptone Soya Broth Vibrio Vagococcus Vibrio Anguillarum Medium virulence array protein gene A Vibrio harveyi... European Association of Fish Pathologists, and the U.K Federation of Culture Collections; and has written previous books on bacterial taxonomy, marine microbiology, methods in aquatic bacteriology, methods for the microbiological examination of fish and shellfish, and pathogens in the environment Dawn Austin is a Research Associate at Heriot-Watt University, a position she has held since 1986 Prior to this... Postulates may be conveniently forgotten So, what are the bacterial fish pathogens? A comprehensive list of all the bacteria, which have been considered to represent fish pathogens, has been included in Table 1.1 (see p 4) Some genera, e.g Vibrio, include many species that are acknowledged to be pathogens of freshwater and/or marine fish species Taxa (highUghted by quotation marks), namely "Catenabacterium'\... (asymptomatic) fish Examples of these "obligate" pathogens include Aer salmonicida and Ren salmoninarum, the causal agents of furunculosis and bacterial kidney disease (BKD), respectively In later chapters, it will be questioned whether or not bacteria should be considered as obligate pathogens of fish, at all It is a personal view that the inability to isolate an organism from the aquatic environment may... interaction between the host (= fish) , the disease-causing situation (= pathogen) and external stressor(s) (= unsuitable changes in the environment; poor hygiene; stress) Before the occurrence of cHnical signs of disease, there may be demonstrable damage to/weakening of the host Yet all too often, the isolation of bacteria from an obviously diseased fish is taken as evidence of infection Koch's Postulates... atypical Aer salmonicida A well-developed ulcer on a Koi carp The aetiological agent was atypical Aer salmonicida An ulcerated goldfish on which the lesion has extended across the body wall, exposing the underlying organs The aetiological agent was atypical Aer salmonicida Carp erythrodermatitis The aetiological agent is Hkely to be atypical Aer salmonicida An ulcer, caused by Vibrio sp., on the surface . y0 w1 h0" alt="" Bacterial Fish Pathogens Diseases of Farmed and Wild Fish B. Austin and D. A. Austin Bacterial Fish Pathogens Diseases of Farmed and Wild Fish Fourth Edition J'V'v. (w/v) crystal violet 232 Requirement for 0.1% (w/v) L-cysteine hydrochloride 233 Oxidation-fermentation test 233 Indole production 233 a-Galactosidase production 233 P-Galactosidase production. by Biolog-GN 222 6.7 Diagnostic traits of the Gram-positive bacterial fish pathogens 225 6.8 Diagnostic traits of the Gram-negative bacterial fish pathogens 227 8.1 Experimental data concerning