z Wild Type Capture Probe-1(WTCP): (It is specifically bound with wild type HBV) 5’ – AAT ACC ACA TCA TCC ATA TA – 3’ z Mutant Type-1 Capture Probe(MT-1CP): (It is specifically bound with mutant type-1 HBVgenome-YVDD) 5’ – AAT ACC ACA TCA TCC AcA TA – 3’ z Mutant Type-2 Capture Probe(MT-2CP):(It is specifically bound with mutant type-2 HBVgenome-YIDD) 5’ – AAT ACC ACA TCA TCa ATA TA – 3’ z Wild type target sequence-1(WTT=YMDD): 5’- TAT ATG GAT GAT GTG GTA TT-3’ z Mutant type-1 target sequence-2(MT-1T=YVDD): 5’- TAT gTG GAT GAT GTG GTA TT-3’ z Mutant type-2 target sequence-3(MT-2T=YIDD): 5’- TAT ATt GAT GAT GTG GTA TT-3’ z Non comlpementary sequences: 5’ – AAT ACC TGT ATT CCT CGC CTG TC –3’ Hybridization detection procedure Cloned Cloned PCR PCR products products z The guanine signal obtained from the wild type probe modified PGE after hybridization with samples 1, 2 and 3 gave a R. S. D. value of 9.4%, 7.6% and 10.2%. z The guanine signal obtained from the hybridization of the Mutant type-1 probe with the amplicons(6 and 1) a R. S. D. value of 10.4% and 11.8%. z The guanine signal obtained from the hybridization of the Mutant type-2 probe with the samples 8 and 9 gave a R. S. D. value of 7.4 % and 6.0%. The detection limits, estimated from S / N = 3, correspond to 457 fmole / mL using 6µg/ml concentration level of target at PGE. DetecTox Development of an SPR-based biosensor for the detection of lipophilic phycotoxins in shellfish residues A European Commission-funded Specific Targeted Research Project Food Quality and Safety Food-CT- 200 4-514055 Biosensors and their application to improving food safety and quality Biosensors and their application to improving food safety and quality The Classic Binding Molecule: The Antibody The Classic Binding Molecule: The Antibody IgG MW ~180,000 SPR SPR Photo-1: (left to right) Assoc. Prof.Arzum ERDEM, Prof. Mehmet OZSOZ, PhD.Std. Kagan KERMAN, PhD Std. Pinar KARA, PhD.Std. Dilsat OZKAN. Photo-2: (left to right) PhD.Std.Burcu MERIC, PhD Std. Pinar KARA, Assoc. Prof.Arzum ERDEM, PhD.Std. Dilsat OZKAN, PhD.Std. Kagan KERMAN. Photo-3: (left to right) Prof. Mehmet OZSOZ and , PhD Std. Hakan KARADENIZ . and 3 gave a R. S. D. value of 9.4%, 7 .6% and 10.2%. z The guanine signal obtained from the hybridization of the Mutant type-1 probe with the amplicons (6 and 1) a R. S. D. value of 10.4% and. samples 8 and 9 gave a R. S. D. value of 7.4 % and 6. 0%. The detection limits, estimated from S / N = 3, correspond to 457 fmole / mL using 6 g/ml concentration level of target at PGE. DetecTox Development. Research Project Food Quality and Safety Food-CT- 200 4-514055 Biosensors and their application to improving food safety and quality Biosensors and their application to improving food safety