ELECTROCHEMICAL DNA BIOSENSORS pot

ELECTROCHEMICAL DNA ELECTROCHEMICAL DNA BIOSENSORS BIOSENSORS Prof. Dr. Mehmet OZSOZ Dept. of Analytical Chemistry, Faculty of Pharmacy, Ege University 35100 Bornova /Izmir, TURKEY ozsozs@pharmacy.ege.edu.tr http://pharmacy.ege.edu.tr/~ozsozs Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Imagination is more important than knowledge. Albert Einstein Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com z If a man emties his purse into his head, no man can take it away from him. An investment in knowledge always pays the best interest. Benjamin Franklin Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com PRESENTATION PRESENTATION OVERVIEW OVERVIEW z Electrochemical DNA Hybridization Sensing Strategies z Inosine based hybridization detection by using carbon paste electrode (CPE) z Detection of NOS-terminator, a genetic element present in GMOs z Carbon Nanotubes z Optical Biosensors Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com z If a man emties his purse into his head, no man can take it away from him. An investment in knowledge always pays the best interest. Benjamin Franklin Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com DNA DNA biosensor scheme biosensor scheme Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Basic principle of Basic principle of a glucose biosensor a glucose biosensor GOX β-D-glucose + O 2 + H 2 O Gluconolactone + H 2 O 2 Transducer Analytical signal Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com K 3 Fe(CN) 6 Screen printed electrode Working electrode reactions pathway ChOx layer Electrochemical mediator Electrode surface (-50mV vs pseudo Ag/AgCl) H 2 O 2 Fe (III) Å Fe (II) Fe (III) + e - Æ Fe (II) Acetylcholine + H 2 O Æ Acetate + Choline (AChE) ChOx Organaphosphate assay Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Electrochemical Electrochemical DNA DNA Hybridization Hybridization Sensing Strategies Sensing Strategies 1.Label based a) Hybridization indicators – metal complexes – organic dye molecules – anticancer agents etc. b) Labelled probe - Metal label (Au or Ag-nanoparticles,) - oligonucleotide containing -SH, -NH2, groups. 2. Label free – Electrochemical signals of DNA purine bases guanine, (Inosine), adenine Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com [...]... strategie Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com How is the probe DNA sequence selected ? Ethidium Bromide Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com HYBRIDIZATION INDICATORS Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com PNA vs DNA Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Label free detection... and Split Unregistered Version - http://www.simpopdf.com Oxidation signal of DNA bases perpendicular to the electrode surface Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Covalent immobilization of Oligonucleotide onto graphite Color shift is only observed from the hybridization with the target DNA Elghanian, R.; Storhoff, J J.; Mucic, R C.; Letsinger, R L.; Mirkin, C A... NOS-terminator, a genetic element present in GMOs - PCR methods, based on exponential amplification of a specific GMO DNA fragment , are highly sensitive, but require sophisticated instruments for post-amplification steps, such as gel electrophoresis and fluorescent imaging Alternatively an electrochemical genosensor is presented here for rapid analysis of GMO related PCR products Simpo PDF Merge and Split... http://www.simpopdf.com Au Electrode Study Procedure Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Electrode system Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com DNA- Chip technology Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Chip Design Factor V Leiden mutation is caused by a point mutation from G to A at the 1691st position of the... What is Factor V Leiden Mutation? When hybridization was occured between probe and target on CPE surface, a guanine oxidation signal at ~+1.00 V was appeared The YES / NO system was established for the electrochemical detection of allele – specific mutation on Factor V Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Experimental Procedure Simpo PDF Merge and Split Unregistered... 1997, 277, 1078-1080 Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com The visible color shift and aggregation of oligonucleotide modified Au nanoparticles upon binding to target DNA is a well-described event Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Simpo PDF Merge and Split... Version - http://www.simpopdf.com Since GMOs and their derivatives are widely used in food industry, the analytical assays are needed to provide the detection and the quanti.cation of GMOs in food matrices DNA extraction and PCR was performed From the amplification of the NOS-terminator region,fragments of 180 bp (base pairs) were obtained Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com... C-3’ Target 5’-GAT TAG AGT CCC CCA ATT AAT CAT T-3’ Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Multi walled carbon nanotubes (MWNTs) were used as nanowires which combined DNA molecules to a carbon paste electrode(CPE) Unique electronic and mechanical properties and chemical stability CNT accelerate the electron transfer Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com . ELECTROCHEMICAL DNA ELECTROCHEMICAL DNA BIOSENSORS BIOSENSORS Prof. Dr. Mehmet OZSOZ Dept. of Analytical Chemistry, Faculty. http://www.simpopdf.com Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Electrochemical Electrochemical DNA DNA Hybridization Hybridization Sensing Strategies Sensing Strategies 1.Label. interest. Benjamin Franklin Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com DNA DNA biosensor scheme biosensor scheme Simpo PDF Merge and Split Unregistered Version - http://www.simpopdf.com Basic