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Luận văn thạc sĩ Công nghệ thực phẩm: Nghiên cứu sản xuất rượu vang giàu chất chống oxy hóa từ quả thanh long Hylocereus polyrhizus bằng nấm men Saccharomyces cerevisiae

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Tiêu đề Nghiên cứu sản xuất rượu vang giàu chất chống oxy hóa từ quả thanh long Hylocereus polyrhizus bằng nấm men Saccharomyces cerevisiae
Tác giả Trương Hữu Uyên Thy
Người hướng dẫn GS.TS. Đống Thị Anh Đào
Trường học Đại học Quốc gia TP. HCM
Chuyên ngành Công nghệ thực phẩm
Thể loại Luận văn thạc sĩ
Năm xuất bản 2017
Thành phố Tp. HCM
Định dạng
Số trang 142
Dung lượng 50,49 MB

Cấu trúc

  • DANH MỤC CÁC CHỮ VIET TAT (16)
    • CHUONG 2. CƠ SỞ KHOA HỌC (35)
    • CHƯƠNG 3. NGUYÊN LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU (39)
    • CHƯƠNG 4. KET QUA NGHIÊN CỨU (55)
    • CHƯƠNG 5. KÉT LUẬN VÀ KIÊN NGHỊ (92)
  • DANH MUC TAI LIEU THAM KHAO (93)
  • KET QUA THU NGHIEM (142)

Nội dung

NHIEM VU VA NOI DUNG: - Phan tích một số thành phan dinh dưỡng trong trái thanh long ruột đỏ Bình Thuận - Khao sát và tối ưu hóa quá trình thủy phân với các yếu t6 tý lệ pha loãng, pH, t

DANH MUC TAI LIEU THAM KHAO

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A Xây dung đường chuẩn A.1 Đường chuẩn Hàm lượng phenolic tổng và độ hấp thu

Simple Regression — Do hap thu A vs Nong do acid gallic

Dependent variable: Do hap thu A Independent variable: Nong do acid gallic (mg/L) Linear model: Y = a + b*X

Least Squares | Standard T Parameter| Estimate Error Statistic | P-Value Intercept |0,0994211 0,00698095 {14,241810,0000 Slope 0,00359774 |0,0000461945 | 77,8825 |0,0000 Analysis of Variance

Source Sum of Squares | Df| Mean Square | F-Ratio | P-Value Model 1,74304 1 |1,74304 6065,68 | 0,0000 Residual 0,00632 194 22|0,000287361

Total (Corr.)| 1,74936 23 Correlation Coefficient = 0,998191 R-squared = 99,6386 percent The output shows the results of fitting a linear model to describe the relationship between A and Nong do acid gallic, The equation of the fitted model is

Do hap thu A = 0,0994211 + 0,00359774*Nong do acid gallic

Plot of Fitted Model A = 0.0994211 + 0.00359774*Nong do acid gallic

0.2 [TT TT [TTT|[TTTỊITTTỊITITT]I mm mnainnnninnnninnnininaning

A.2 Đường chuẩn Hoạt tính chống oxy hóa DPPH va độ hap thu

Simple Regression — Do hap thu A vs Nong do Trolox

Dependent variable: Do hap thu A Independent variable: Nong do Trolox (mg/L) Linear model: Y = a + b*X

Least Squares | Standard T Parameter| Estimate Error Statistic |P-Value Intercept |0,540548 0,00449402 [120,281 |0,0000 Slope -0,00245048 | 0,0000386784 | -63,3552]0,0000 Analysis of Variance

Source Sum of Squares | Df|Mean Square |F-Ratio |P-Value Model 0,378305 1 |0,378305 4013,88]0,0000 Residual 0,00150799 16]0,0000942491

Total (Corr.)|0,379813 17 Correlation Coefficient = -0,998013 R-squared = 99,603 percent The output shows the results of fitting a linear model to describe the relationship between A and Nong do Trolox The equation of the fitted model is

Do hap thu A = 0,540548 - 0,00245048*Nong do Trolox

Plot of Fitted Model A = 0.540548 - 0.00245048*Nong do trolox

B Xử lý số liệu các thí nghiệm khảo sát quá trình thủy phân

B.1 Khảo sát tỷ lệ pha loãng thịt quả/nước (w/w)

Bảng B.1 Kết quả thi nghiệm khảo sát tỷ lệ pha loãng thịt gquả/nước

Ty lệ thịt Hiệu suất thu Hàm lượng Hoạt tính chống oxy quả/nước hồi chất khô phenolie tổng hóa DPPH

Hiệu suất thu hôi chất khô H%

Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range} Stnd Stnd. deviation variation skewness kurtosis 1/0 |3 68,0633 | 1,81814 2,67125% 66,04 69,56 3,52 |-0,844391

ANOVA Table for H% by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups |622,712 3 |207,571 124,67 |0,0000 Within groups | 13,3194 8 |1,66492

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD

Mau \Count|Mean | Homogeneous Groups 1/0 |3 68,0633 | X

Summary Statistics for Phenolic tổng

Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis 1/0 |3 440.333 |5.45191 1.23813% 434.5 445.3 10.8 |-0.493043

ANOVA Table for Phenolic tong by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 83960 1 3 |27986.7 537.77 10.0000 Within groups |416.34 8 52.0425

Multiple Range Tests for Phenolic tong by Mau

Mau |\Count|Mean _|Homogeneous Groups 1/1.5]3 214.4 |X

Hoạt tính chống oxy hóa DPPH

Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis 1/0 |3 304.167 |3.4990S 1.15037% 301.4 308.1 6.7 10.943002

ANOVA Table for DPPH by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups} 71477.0 3 |23825.7 971.19 10.0000 Within groups | 196.26 8 |24.5325

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |\Count|Mean _|Homogeneous Groups 1/1.5]3 95.5333 |X

Bang B.2 Kết quả thi nghiệm khảo sát pH pH Hiệu suất thu hồi Hàm lượng phenolie Hoạt tính chống oxy hóa chất khô (%) tổng (mgGAE/L) DPPH (mgTEAC/L) 4.0 81,5+(,9*° 334,744 196, 1+3,3°

Hiệu suất thu hồi chat khô H%

Mau |Count| Average | Standard Coeff of | Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness |kurtosis 4,0 |3 81,47 {0,91 1,11698% 80,56 82,38 1,82 10,0

ANOVA Table for H% by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 23,7821 3 |7,92736 5,18 0,0280 Within groups | 12,2463 8 |1,53079

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD

Mau\Count|Mean | Homogeneous Groups 5,5 |3 7957 |X

Summary Statistics for Phenolic tổng

Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis 4 3 334.7 {4.15812 1.24234% 330.2 338.4 8.2 -0.589535

ANOVA Table for Phenolic tong by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 748.357 3 1249.452 7.74 0.0095 Within groups |257.92 8 }32.24

Multiple Range Tests for Phenolic tong by Mau

Mau}\Count|Mean _|Homogeneous Groups 5.5 |3 330.167|X

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis

ANOVA Table for DPPH by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 708.549 3 |236.183 21.60 10.0005 Within groups |87.48 8 |10.935

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau)\Count|Mean | Homogeneous Groups

B.3 Khao sát tỷ lệ enzyme pectinase/cellulase (v/v)

Bang B.3 Kết qua thi nghiệm khảo sát ty lệ enzyme Pectinase/Cellulase

Ty lệ enzyme Hiệu suất thu héi Ham lượngHoạt tính chống Pectinase/Cellulase chat khô (3%) phenolic tngoxy hóa DPPH

Hiệu suất thu hôi chất khô H%

Summary Statistics for H% mau _ |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis 0/0 |3 62.1533 |0.735006 1.18257% 61.42 62.89 1.47 |0.0144303

ANOVA Table for H% by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups} 1017.36 7 {145.337 81.10 10.0000 Within groups |28.6749 16| 1.79218

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD mau|Count|Mean _ | Homogeneous Groups 0/0 |3 62.1533|X

Summary Statistics for Phenolic tổng

Multiple Range Tests for Phenolic tong by Mau

Method: 95.0 percent LSD mau \Count |Mean Homogeneous Groups 0/0 43 303.513 |X

0/1 3 309893 | X 1/0 ]3 312.51 XX 153 43 316.997 XX 3/1 3 317.467 X J2 l3 322.843 xX 2/1 3 323.477 X 1/1 3 332.28 X mạam |Count|Average|Standard Coeff of |Minimum| Maximum | Range |Stnd Stnd. deviation variation skewness kurtosis 0/0 43 303.513 [2.78857 0.918764% 301.52 |306.7 5.18 {1.11232

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |1683.7 7 |240.528 31.72 0.0000 Within groups 121.327 l6 |7.58292

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau |Count| Average |Standard Coeff of |Minimum| Maximum | Range |Stnd Stnd. deviation variation skewness kurtosis 0/0 |3 195.12 |2.20959 1.13243% 19321 |197.54 |4353 {0.695312

ANOVA Table for DPPH by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |481.099 7 {68.7284 50.08 0.0000 Within groups |21.9575 16 |1.37235

Multiple Range Tests for DPPH by Mau

Method: 95.0 percent LSD Mau |\Count |Mean Homogeneous Groups

B.4 Khao sát ham lượng enzyme tổng (%v/dwt) Bảng B.4 Kết quả thi nghiệm khảo sát hàm lượng enzyme

Hàm lượng Hiệu suất thu Hàm lượng enzyme hồi chất khô phenolic tong

Hoạt tính chống oxy hóa DPPH (mgTEAC/L)

Hiệu suất thu hồi chất khô H%

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range | Sind Stnd. deviation variation skewness kurtosis 0% |3 63.0933 | 1.09715 1.73893% 61.84 63.88 2.04 |-1.10858

ANOVA Table for H% by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups} 1192.91 4 1298.227 146.95 10.0000 Within groups |20.2947 1012.02947

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD

Mau}\Count|Mean _|Homogeneous Groups 0% |3 63.0933 |X

Summary Statistics for Phenolic tổng

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range | Sind Stnd. deviation variation skewness _ | kurtosis 0% |3 306.733 | 4.65224 1.5167% 301.5 310.4 8.9 |-0.950031

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups |3418.9 4 1854.724 1637 10.0002 Within groups |522.14 10152.214

Multiple Range Tests for Phenolic tổng by Mau

Mau}\Count|Mean _|Homogeneous Groups 0% |3 306.733|X

Hoạt tính chống oxy hóa DPPH

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range |Stnd Stnd. deviation variation skewness _ | kurtosis 0% |3 194.733 |4.96622 2.55027% 190.5 200.2 97 {0.74149

ANOVA Table for DPPH by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 5528.6 4 |1382.15 52.88 0.0000 Within groups |261.393 10}26.1393

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau}\Count|Mean _|Homogeneous Groups 0% |3 194.733] X

Bang B.5 Kết qua thi nghiệm khảo sát nhiệt độ thủy phân

Khảo sát nhiệt độ thủy phân

Nhiệt Hiệu suấtthuhồi Hàm lượng phenolic Hoạt tính chống oxy hóa tông (mgGAE/L) độ chất khô (%)

Hiệu suất thu hồi chất khô H%

Mau |Count| Average | Standard Coeff of | Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis 40 {3 81.2467 | 1.62488 1.99993% 79.51 82.73 3.22 |-0.48404

ANOVA Table for H% by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 76.4764 4 119.1191 9.98 0.0016 Within groups |19.1635 10|1.91635

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD

Mau}\Count|Mean _|Homogeneous Groups 40 |3 81.2467} X

Summary Statistics for Phenolic tổng

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range |Stnd Stnd. deviation variation skewness kurtosis 40 {3 317.767 | 6.26126 1.97039% 312.8 324.8 12.0 |0.935857

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 5042.75 4 11260.69 15.28 10.0003 Within groups |825.187 10|82.5187

Multiple Range Tests for Phenolic tong by Mau

Method: 95,0 percent LSDMau|Count Mean Homogeneous Groups40 |3 317 767 X

Hoạt tính chống oxy hóa DPPH

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis 40 {3 210.333 | 9.9631 4.73681% 199.4 218.9 19.5 |-0.713207

ANOVA Table for DPPH by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between sroups |2810.72 4 1702.679 21.01 10.0001 Within groups |334.387 1033.4387

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau}\Count|Mean _|Homogeneous Groups 40 |3 210.333] X

B.6 Khảo sát thời gian thủy phân

Bảng B.6 Kết quả thi nghiệm khảo sát thời gian thủy phân Thòigian Hiệu suất thu

(phút) hôi chất khô (%) tong (mgGAE/L)

Hàm lượng phenolic Hoạt tinh chống oxy

Hiệu suất thu hồi chất khô H%

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range | Sind Stnd. deviation variation skewness | kurtosis 0 3 4833 | 1.82502 3.77617% 46.51 50.16 3.65 10.0174348

ANOVA Table for H% by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups |3907.09 5 {781.418 316.47 10.0000 Within groups |29.6303 12|2.46919

Multiple Range Tests for H% by Mau Method: 95,0 percent LSD

Mau\Count|Mean | Homogeneous Groups

Summary Statistics for Phenolic tổng Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis

ANOVA Table for Phenolic tong by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups} 11390.6 5 {2278.12 47.63 10.0000 Within groups |573.98 12 |47.8317

Multiple Range Tests for Phenolic tổng by Mau

Mau}\Count|Mean _|Homogeneous Groups 0 3 285.9 |X

Hoạt tính chống oxy hóa DPPH

Mau |Count| Average | Standard Coeff of Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis 0 3 181.68 |3.54208 1.94962% 178.21 {185.29 |708 |0.125571

ANOVA Table for DPPH by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 14691.7 5 [2938.34 135.61 10.0000 Within groups |260.002 12|21.6669

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau}\Count|Mean _|Homogeneous Groups 0 3 181.68 |X

C Xử lý số liệu các thí nghiệm khảo sát lên men chínhC.1 Khảo sát hàm lượng chất khô hòa tan ban đầu

Bảng C.1 Kết quả thí nghiệm khảo sát hàm lượng chất khô hòa tan ban đầu

Hàm lượng chất Hàm lượng Hàm lượng Hoạt tính chong khô hòa tan ban ethanol (%v/v) | phenolic tong oxy hóa DPPH dau (Bx) (mgGAE/L) (mgTEAC/L)

Summary Statistics for Ethanol Mau |\Count|Average|Standard Coeff of Minimum|Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis 16Bx|3 7.46667 {0.11547 1.54647% 7.4 7.6 0.2 1.22474

18Bx|3 7.06667 |0.11547 1.63401% 7.0 7.2 0.2 1.22474 20Bx|3 6.53333 |0.11547 1.7674% 6.4 6.6 0.2 |-1.22474 22Bx|3 6.06667 |0.11547 1.90335% 6.0 6.2 0.2 1.22474 24Bx|3 5.53333 |0.11547 2.08681% 5.4 5.6 0.2 |-1.22474 Total | 15 6.53333 |0.719788 11.0172% 5.4 7.6 2.2 |-0.142883 |-I.01939

ANOVA Table for Ethanol by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |7.12 4 1.78 133.50 0.0000 Within groups |0.133333 10 |0.0133333

Multiple Range Tests for Ethanol by Mau

Method: 95.0 percent LSD Mau |Count |Mean Homogeneous Groups 24Bx |3 5.53333 |X

Summary Statistics for Phenolic tổng Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range} Stnd Stnd. deviation variation skewness kurtosis 16Bx|3 258,433 |5,36315 2,07525% 253,3 264,0 10,7 |0,25542

18Bx]3 265,2 |4,16053 1,56883% 260,6 268,7 8,1 -0,782475 20Bx|3 269,767 |4,45009 1,64961% 265,3 274,2 8,9 |-0,0238332 22Bx|3 275,367 | 11,0636 4,01777% 264,0 286,1 22,1 |-0,181555 24Bx|3 278,267 |5,09346 1,83042% 274,7 284,1 9,4 1,1356 Total] 15 269,407 |9,20105 3,4153% 253,3 286,1 32,8 |0,316941 -0,292655

ANOVA Table for Phenolic tong by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 756,783 4 |189,196 4,42 0,0259 Within groups |428,447 10|42,8447

Multiple Range Tests for Phenolic tong by Mau

Method: 95,0 percent LSD Mau |\Count|Mean | Homogeneous Groups

16Bx|3 258,433|X 18Bx]3 2652_ |XX 20Bx|3 269,767 | XXX 22Bx|3 275,367| XX 24Bx|3 278,267| X

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range} Stnd Stnd. deviation variation skewness kurtosis 16Bx|3 156,167 2,75015 1,76104% 153,4 158,9 5,5 -0,0385617

18Bx]3 157,367 |3,48473 2,2144% 154,2 161,1 6,9 |0,50375320Bx|3 164,067 |3,27465 1,99593% 160,4 166,7 6,3 -0,90410822Bx|3 162,267 |3,80701 2,34614% 158,6 166,2 7,6 {0,22179224Bx|3 161,733 | 1,80093 1,11352% 159,9 163,5 3,6 |-0,117629Total] 15 160,32 |4,0859 2,54859% 153,4 166,7 13,3 |-0,13248 -0,598351

ANOVA Table for DPPH by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups] 137,391 4 1343477 3,57 0,0469 Within groups |96,3333 1019,63333

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |\Count|Mean | Homogeneous Groups 16Bx|3 156,167|X

18Bx]3 157,367| XxX 24Bx|3 161,733|XXX 22Bx|3 162,267| XX 20Bx|3 164,067] xX

C.2 Khao sat ham lượng men

Bang C.2 Kết qua thí nghiệm khảo sát hàm lượng men

Hàm lượng men Hàm lượng Hàm lượng Hoạt tính chống (Yow/w) ethanol (“ov/v) | phenolic ting oxy hóa DPPH

Summary Statistics for Ethanol Mau |Count|Average |Standard Coeff of Minimum | Maximum |Range|Stnd Stnd. deviation variation skewness kurtosis 0% |3 0.133333|0.11547 86.6025% 0.0 0.2 0.2 |-1.22474

ANOVA Table for Ethanol by Mau

Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups {149.21 3 {49.7367 2486.83 0.0000 Within groups |0.16 0.02

Multiple Range Tests for Ethanol by Mau

Method: 95.0 percent LSD Mau Count |Mean Homogeneous Groups

Summary Statistics for Phenolic tổng

Mau_|Count|Average | Standard deviation| Coeff of variation| Minimum |Maximum| Range |Stnd skewness 0% |3 266,5 |2,0664 0,775384% 264,3 268,4 4,1 -0,452224 0,02%|3 269,633 |4,2501 1,57625% 265,4 273,9 8,5 0,0249546 0,04%|3 2727 |1,15326 0,422903% 271,5 273,8 2,3 -0,273838 0,0ó%|3 275,4 |4,25088 1,54353% 271,1 279,6 8,5 -0,0748132 Total H12 271,058 |4,43672 1,63681% 264,3 279,6 15,3 |0,301718

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 133,063 3 144,3542 4,25 0,0451 Within groups [83,4667 8 |10,4333

Multiple Range Tests for Phenolic tong by Mau

Method: 95,0 percent LSD Mau |Count\|Mean _ | Homogeneous Groups 0% l3 266,5 |X

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau_|Count|Average | Standard deviation| Coeff of variation| Minimum |Maximum| Range |Stnd skewness 0% |3 146,633 |5,78475 3,94504% 141,7 153,0 11,3 |0,74002 0,02%|3 150,167 |5,27857 3,51514% 144,6 155,1 10,5 |-0,376284 0,04%|3 162,233 |7,8015 4,80881% 155,0 170,5 15,5 |0,41407 0,0ó%|3 164,333 |7,35006 4,47265% 156,2 170,5 14,3 |-0,790452 Total H12 155,842 |9,73424 6,24624% 141,7 170,5 28,8 |0,333009

ANOVA Table for DPPH by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 689,883 3 |229,961 5,22 0,0275 Within groups |352,427 8 |44,0533

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |Count\|Mean _ | Homogeneous Groups 0% l3 146,633 |X

Bảng C.3 Kết qua thí nghiệm khảo sát nhiệt độ lên men chính

Ham lượng phenolic Hoat tinh chong tong (mgGAE/L) oxy hóa DPPH

Summary Statistics for Ethanol Mau |\Count|Average|Standard Coeff of Minimum|Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis I5oC|3 4.4 0.0 0.0% 4.4 4.4 0.0

ANOVA Table for Ethanol by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups | 10.6233 3 {3.54111 212.47 0.0000 Within groups |0.133333 8 0.0166667

Multiple Range Tests for Ethanol by Mau

Method: 95.0 percent LSD Mau |Count |\Mean Homogeneous Groups

Summary Statistics for phenolic tong Mau |Count| Average | Standard Coeff of |Minimum | Maximum | Range} Stnd Stnd. deviation variation skewness kurtosis 150C}3 265,1 |9,36002 3,53075% 256,0 274,7 18,7 |0,169492

ANOVA Table for phenolic tổng by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 1228,17 3 409,389 5,23 0,0273 Within groups [625,913 8 |78,2392

Multiple Range Tests for phenolic tong by Mau

Method: 95,0 percent LSD Mau |\Count|Mean | Homogeneous Groups

15oC |3 2651 |X 30oC |3 268,367|X 25oC |3 278,733|XX 20oC |3 291,0 xX

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau |Count| Average | Standard Coeff of | Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness | kurtosis 150C}3 171,6 |4,27551 2,49156% 166,8 175,0 8,2 -0,930211

ANOVA Table for DPPH by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups |582,39 3 1194,13 7,51 0,0103 Within groups |206,927 8 |25,8658

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |\Count|Mean | Homogeneous Groups 30oC |3 154,633|X

25oC |3 163,8 |XX20oC |3 171,5 xX15oC |3 171,6 xX

C.4 Khảo sát thời gian lên men chính

Bảng C.4 Kết quả thí nghiệm khảo sát thời gian lên men chính Thời gian Hàm lượng Hàm lượng phenolic Hoạt tính chống oxy

(ngày) ethanol (“ov/v) tong (mgGAE/L) hoa DPPH

Summary Statistics for Ethanol Mau |Count|Average|Standard Coeff of Minimum | Maximum | Range| Sind Stnd. deviation variation skewness | kurtosis 2 ngày |3 3.06667 |0.11547 3.76533% 3.0 3.2 0.2 1.22474

ANOVA Table for Ethanol by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |100.917 3 [33.6389 1261.46 0.0000 Within groups |0.213333 8 0.0266667

Total (Corr.) 101.13 11 Multiple Range Tests for Ethanol by Mau Method: 95.0 percent LSD

Mau Count |Mean Homogeneous Groups 2ngay |3 3.06667 |X

Hàm lượng Phenolic tông Summary Statistics for Phenolic tổng

Mau |Counf| Average | Standard deviation| Coeff of variation |Minimum | Maximum | Range | Stnd skewness2 ngày |3 275,0 |5,85577 2,12937% 269,3 281,0 11,7 |0,162594 ngày |3 277,833 |4,80555 1,72965% 272,9 282,5 9,6 |-0,1760296 ngày |3 280,3 |0,7 0,249732% 279,6 281,0 1,4 109,08 ngày |3 287,267 |3,98037 1,3856% 283,6 291,5 7,9 |0,443822Total |12 280,1 |5,99303 2,1396% 269,3 291,5 22,2 10,0205391

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups |247,647 3 |82,5489 4,48 0,0399 Within groups | 147,433 8 |18,4292

Multiple Range Tests for Phenolic tong by Mau

Method: 95,0 percent LSD Mau |Count|Mean |Homogeneous Groups 2 ngày |3 2750 |X

Hoạt tính chống oxy hóa DPPH

Summary Statistics for DPPH Mau |Counf| Average | Standard deviation| Coeff of variation |Minimum | Maximum | Range | Stnd skewness 2 ngày |3 164,9 16.8942 4,18084% 158,5 172,2 13,7 |0,408311 4 ngày |3 174,967 15,4151 3,09493% 170,1 180,8 10,7 |0,549924 6 ngày |3 183,867 |6,26285 3,40619% 178,4 190,7 12,3 |0,6613 8 ngày |3 185,033 |5,30786 2,86859% 179,9 190,5 10,6 10,19904 Total |12 177,192 |9,88456 5,57845% 158,5 190,7 32,2 |-0,619202

ANOVA Table for DPPH by Mau Source Sum of Squares | Df|Mean Square | F-Rafio| P-Value Between groups | 786,249 3 |262,083 7,27 0,0113 Within groups |288,5 8 |36,0625

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |Count|Mean |Homogeneous Groups 2 ngày |3 1649 |X

D Anh hưởng của các thông số đến hàm lượng phenolic tổng (quá trình lên men phụ)

D.1 Khảo sát nhiệt độ lên men phụ

Bảng D.1 Kết quả thí nghiệm khảo sát nhiệt độ lên men phụ

Nhiệt Hàm lượng phenolic tổng Hoạt tính chống oxy hóa DPPH độ (mgGAE/L) (mgTEAC/L) °C 266,4+4,8 175,4+3,42

Summary Statistics for Phenolic tổng

Mau |Coumi| Average |Standard Coeff of Minimum|Maximum | Range} Sind Stnd. deviation variation skewness _ | kurtosis 100C|3 261.567 |3.00888 1.15033% 258.7 264.7 6.0 |0.279793

ANOVA Table for Phenolic tổng by Mau

Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |3142.82 4 1785.704 29.07 0.0000 Within groups |270.273 10 |27.0273

Multiple Range Tests for Phenolic tong by Mau

Mau |Count |\Mean Homogeneous Groups 250C |3 228.367 |X

Hoạt tính chống oxy hóa DPPH

Mau |Coumi| Average |Standard Coeff of Minimum|Maximum | Range} Sind Stnd. deviation variation skewness kurtosis 100C]|3 1739 |3.24191 1.86424% 171.0 177.4 6.4 |0.568736

I5oC|3 1722 |2.227II 1.29333% 170.2 174.6 4.4 10.553065 200C}3 165.567 |2.31805 1.40007% 162.9 167.1 4.2 |-1.18384 25oC|3 162.267 | 1.97569 1.21755% 160.5 164.4 3.9 |0.570202 5oC |3 175.4 |3.38674 1.93087% 172.7 179.2 6.5 |0.924469 Total} 15 169.867 |5.72072 3.36777% 160.5 179.2 18.7 |-0.261817 |-0.785739

ANOVA Table for DPPH by Mau

Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups [385.74 4 96.435 13.31 0.0005 Within groups |72.4333 10 |7.24333

Multiple Range Tests for DPPH by Mau Method: 95,0 percent LSD

Mau |Couní |\Mean Homogeneous Groups 250C |3 162.267 |X

D.2 Khảo sát thời gian lên men phụ

Bảng D.2 Kết quả thí nghiệm khảo sát thời gian lên men phụ

(mgGAE/L) gian Ham lượng phenolic tổng

Hoạt tinh chống oxy hóa DPPH

Summary Statistics for Phenolic Mau |Count| Average |Standard Coeff of Minimum|Maximum | Range | Stnd Stnd. deviation variation skewness _ |kurtosis IS 43 272.233 | 6.86246 2.5208% 264.9 278.5 13.6 }|-0.482642

ANOVA Table for Phenolic by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |358.951 4 89.7377 4.35 0.0270 Within groups |206.133 10 {20.6133

Multiple Range Tests for Phenolic by Mau Method: 95.0 percent LSD

Mau |\Count |Mean Homogeneous Groups 35 3 258.967 |X

Hoạt tinh chong oxy hóa DPPH

Summary Statistics for DPPH Mau |Count| Average |Standard Coeff of Minimum | Maximum | Range | Stnd Stnd. deviation variation skewness kurtosis IS 43 180.067 |4.24774 2.35898% 175.5 183.9 8.4 |-0.532966

ANOVA Table for DPPH by Mau Source Sum of Squares \Df |Mean Square |F-Ratio P-Value Between groups |245.617 4 161.4043 3.83 0.0387 Within groups 160.307 10 {16.0307

Multiple Range Tests for DPPH by Mau Method: 95.0 percent LSD

Mau |Count |Mean Homogeneous Groups 35 3 168.9 x

E Phương pháp xác định các thành phan nguyên liệu

E.1 Phương pháp xác định hàm lượng pectin (phương pháp Ca-pectat) [A310]

Chuẩn bị: dung dịch CH3COOH IN, dung dich NaOH 0,1N, dung dịch CaCl2 2N (cân 230g CaCl trong 1 lít nước cất, khuấy đều và lọc can thận)

Cân 20g nguyên liệu thanh long, giã nhỏ rồi cho vào 100mL nước cat, gia nhiệt lên 40 + 45°C, sau 2 + 3 giờ, lay dịch chiết lần thứ nhất, tương tự cho lần 2 và 3 sao cho tong thé tích dịch chiết là 200mL.

Ngày đăng: 09/09/2024, 06:08

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