Báo cáo nghiên cứu khoa học " Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs - Milestones 7 " docx

Ministry of Agriculture & Rural Development Progress Report Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs. Milestones 7: 4 th 6 monthly Report Date 01/01/2007 to 30/06/2007 Table of Contents 1. Institute Information ____________________________________________ 3 2. Project Abstract _______________________________________________ 4 3. Executive Summary ____________________________________________ 4 4. Introduction & Background ______________________________________ 5 5. Progress to Date _______________________________________________ 6 5.1 Implementation Highlights ___________________________________ 6 5.2 Smallholder Benefits ________________________________________ 7 5.3 Capacity Building___________________________________________ 8 5.4 Publicity __________________________________________________ 8 5.5 Project Management ________________________________________ 8 6. Report on Cross-Cutting Issues __________________________________ 8 6.1 Environment_______________________________________________ 8 6.2 Gender and Social Issues ____________________________________ 8 7. Implementation & Sustainability Issues ____________________________ 9 7.1 Issues and Constraints ______________________________________ 9 7.2 Options ___________________________________________________ 9 7.3 Sustainability ______________________________________________ 9 8. Next Critical Steps ____________________________________________ 10 9. Conclusion __________________________________________________ 10 10. Statuatory Declaration _________________________________________ 11 11. Project progress against proposed objectives, outputs, activities and inputs___________________________________________________ 16 1. Institute Information Project Name Vietnamese Institution Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam. Vietnamese Project Team Leader Dr. Dong Manh Hoa Australian Organisation A ustralian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Australian Personnel Mr Chris Morrissy Date commenced 01/06/2005 Completion date (original) 01/06/2008 Completion date (revised) Reporting period 01/07/2006 – 31/12/2006 Contact Officer(s) In Australia: Team Leader Name: Mr Chris Morrissy Telephone: +61 3 5227 5000 Position: Diagnostic Virologist Supervisor Mammalian Virology Fax: +61 3 5227 5555 Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Email: chris.morrissy@csiro.au In Australia: Administrative contact Name: Mr Chris Morrissy Telephone: +61 3 5227 5000 Position: Patents Contracts Officer Fax: +61 3 5227 5555 Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Email: chris.morrissy@csir o.au In Vietnam Name: Dr. Dong Manh Hoa Telephone: + 84 8 8568220 Position: Director Fax: + 84 8 8569050 Organisation Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam. Email: rahchcmc@hcm.vnn.v n 2. Project Abstract The project’s purpose is to determine why there are vaccine failures, and to investigate what serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for these serotypes. Regional laboratories are setup with the reagents and methods to allow a diagnostic capability for FMDV diagnosis and serology. Control strategies for understanding of FMD epidemiology have been implemented through veterinary and laboratory training workshops and laboratory testing for both virus typing and sero- surveillance have begun. The project has highlighted the importance of having a laboratory network to identify what is happening in the field and how to prevent and control disease outbreaks. The pilot zones are established in provinces near the borders of Vietnam to study serotypes circulating in Vietnam and to determine their origin. The quality and collection of samples have increased giving more data on the FMD situation in Vietnam. Molecular epidemiological studies of the FMDV isolates in these provinces will provide insights to the effectiveness of border control and the origin of FMDV circulating in Vietnam each year. Virus isolation and molecular studies have started to be carried out on FMD samples from the field. A diagnostic capacity for FMD would allow the early detection and identification of disease enabling better control of disease and help reduce loss of livestock and so productivity. 3. Executive Summary The main focus of this reporting period was to improve the data collected from the field studies for sero-surveillance and from disease investigations. The Regional Animal Health Centre in Ho Chi Minh City continued to train and support the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals for the villages in the project. The data on the animals from the sero-surveillance is critical to allow analysis of the data and for making decisions on cause of vaccine failure. The establishment FMD diagnostics in the collaborating laboratories is continuing with the HCMC laboratory having FMD diagnostics established for virus isolation, ELISA and PCR. The HCMC laboratory is preparing its own ELISA antigen for use in the project as part of training to become self sufficient. One scientist from the Regional Animal Health laboratory HCMC and one scientist from NCVD Hanoi trained at AAHL in FMD diagnostics, with a focus on cell culture and QA. Two AAHL consultants also carried out training at the HCMC and Hanoi laboratories with one consultant also visiting the laboratories at Can Tho and Da Nang. The focus of the visit to the four collaborating laboratories was to implement the use of the Vietnam antigen produced at HCMC into the FMD ELISAs. The first consultant reviewed virus isolation, molecular and ELISA technologies for diagnosis of FMD and reviewed ELISA results from the sero- surveillance. Quality Assurance (QA) was again a important part of the training and QA records were reviewed during training visit to each laboratory. The project has been asked to attend a number of national meetings to advise the Vietnamese Government and DAH on control of FMD. Avian Influenza (AI) continues to delay the sero-surveillance in the field as field staff deal with the AI outbreak. . 4. Introduction & Background Objectives of the project: 1. To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance. 2. To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies. Completing these objectives will improve the diagnostic capability of the Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control. This will strengthen the profile of DAH which will play a vital role in making Vietnam more economically competitive. Improved animal health will lead to an increase in rural productivity though increased animal production and indirectly in increased crop production. Healthy animals will enable small farmers to be more competitive in the local market. Control of FMD and animal diseases in general will give poor farmers a more stable income stream and reduce their vulnerability to natural and economic problems. Establishing a diagnostic network which extends from the North to South Vietnam, from the laboratory to the farm level, reinforced by training and education, will give Vietnam a working model on which to base disease control. This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast. Implementation Approach and Strategy The project approach is thought to be the most appropriate for developing an understanding of FMD epidemiology in Vietnam. The approach for technology transfer is well established at AAHL and has been successfully applied in previous projects in Vietnam, Thailand and Indonesia. The field studies and epidemiology and sero-surveillance approaches have been designed and planned in conjunction with DAH to provide the maximum necessary information to demonstrate the FMD situation in Vietnam and the effectiveness of FMD vaccines. The diagnostic technologies that will be used in this approach are the standard diagnostic tests in use throughout the world to study FMD as directed by OIE. AAHL has a lot of experience with field surveys for prevalence of antibodies, as in the ACIAR projects in Laos and Thailand on FMD. The Philippines is another example where OIE standard diagnostic tests are being used to control and eradicate FMD. 5. Progress to Date 5.1 Implementation Highlights Main achievements for this reporting period were: • Further training of field veterinarians was carried out with feedback of the results and experiences from first two rounds of testing. The aim was to increase the quality of sample and data collection to ensure issues of vaccine failure can be addressed. • Training of laboratory staff at AAHL, one scientist from the RAHO-HCMC and one scientist from NCVD-Hanoi trained at AAHL in FMD diagnostics, with a focus on cell culture and QA. The scientist from Hanoi also trained in FMD ELISA. Training was carried out under a quality system emphasizing the importance of Quality Assurance in the laboratory focusing on test maintenance, record keeping and data collection. • A consultant from AAHL (project leader) reviewed data from the field in the HCMC laboratory and outlined the improved required in data collection. A plan was made to retest samples to compare antibody titres for samples from the provinces to allow identification by serology of the circulating isolate. • AAHL consultant reviewed cell culture and virus isolation for growth of FMD isolates from the field. Cell culture is important to grow FMD virus to allow further analysis of FMD field isolates by PCR and sequencing. ELISA and molecular techniques were also reviewed. Again Quality Assurance, record keeping and data collection was emphasized. • The consultant from AAHL also reviewed the transfer of ELISA technology to each laboratory and the implementation of a Quality Assurance system into the laboratory. The ELISA antigen from HCMC was taken to each laboratory to compare the use of this Vietnam antigen to the antigen from AAHL. The antigen from Vietnam was found to give similar results and the production of antigen was a success. • Sampling for next round sera collection completed for each province for epidemiological studies and sero-surveillance. The collection of tissue samples from outbreaks continued to increase giving valuable information on the serotypes circulating in Vietnam • Virus isolation and cell culture in the process of being setup at the Hanoi laboratory. • The supply of consumables and further reagents for testing of samples by FMD ELISA, molecular technology and cell culture were supplied as requested. Report from Vietnam: ACTIVITIES from Jan 1 st 2007 to May 30 th 2007 1.Training No. Date & place Subject Person in charge Remark 1 March, 2007 - Expert from AAHL working in Viet Nam - Chris Morrissy - Darren Schafer - Testing and validation the first batch of FMDV serotype O, A and Asia 1 ELISA antigen produced by RAHC-HCMC 2 April 18 – May 16, 2007, Australia - ELISA in advance training - Virus isolation - Chris Morrissy - Lynda Wright - 1 Participant from RAHC- HCMC 2. Field activities 2.1.1. Visit to 8 provinces in the project (Middle and South Vietnam), March. 2007. • Transfer the equipments and documents ( form, questionnaire) for sample collection. • Transfer money for sample collection. • Feed back the results from third testing round and share the experience on sample collection with the field veterinarian staff to increase the quality of collected samples . • Staff in charge : Dr. Hoa, Dr. Vu , Dr. Ha ( RAHC-HCMC) - Dr. Quan , Dr.Quang (RAHC-Da Nang) – Dr. Thanh , Dr. Dung ( RAHC-Can Tho) 2.1.2. Visit to 2 provinces in the project (North Vietnam ), March. 2007 • Transfer the equipments and documents ( form, questionnaire) for sample collection. • Transfer money for sample collection. • Feed back the results from third testing round and share the experience on sample collection with the field veterinarian staff to increase the quality of collected samples . • Staff in charge : Dr. Cam , Dr. Tung ( NVDC- Ha Noi) 2.2. Collect sera samples from 10 provinces for the four round of surveillance , 120 cattle sera and 120 pig sera from each province. 2.2.1 NVDC-Ha Noi : 02 provinces (Quang Ninh and Lang Son) , total 480 sera samples. 2.2.2 RAHC-Da Nang : 02 provinces (Kontum and Quang Nam) , total 480 sera samples. 2.2.3 RAHC-Can Tho : 02 provinces (An Giang, Kien Giang ), toatl 480 sera. 2.2.4 RAHC-HCMC : 04 provinces ( Binh Phuoc, Tay Ninh, Long An, Dong Thap), total 960 sera 2.3 Collect tissue samples for FMDV typing 2.3.1 NVDC-Ha Noi : 98 tissue samples from the North , 2.3.2 RAHC-HCMC : 123 tissue samples from the South . 3. Laboratory Activities 3.1 National Vaterinary Diagnostic Center- Ha Noi ( NVDC-Ha Noi): 3.1.1 Testing on tissue samples for FMDV typing : 98 samples, 89 of the are serotype O and 9 are serotype Asia 1. 3.1.2 Testing on sera for FMDV antibodies detection : 480 sera , test finished 3.2 Regional Animal Health Center Da Nang (RAHC-Da Nang) 3.2.1 Sending 480 sera to RAHC-HCMC for retest : 480 sera 3.3 Regional Animal Health Center Can Tho (RAHC-Can Tho) 3.2.1 Sending 480 sera to RAHC-HCMC for retest : 480 sera 3.4 Regional Animal Health Center HCMC(RAHC-HCMC) 3.4.1 Testing on tissue samples for FMDV typing : 123 samples, 01 of them is serotype A , 116 samples are serotype O and 06 samples are negative Remark: test is cover 03 serotype : serotype A, O and Asia 1 3.4.2 Testing on sera for FMDV antibodies detection : 1920 sera ( 960 sera in the region and retest 960 sera from RAHC-Can Tho and Da Nang ). Test finished 3.4.3 Isolate FMDV by BHK cell line from 20 selected samples , 01 serotype A and 19 serotype O. 3.4.4 Production FMDV ELISA antigen: Produce the first batch of FMDV antigen • Serotype O: 35 ml • Serotype A : 35ml • Serotype Asia 1: 35ml Testing this antigen with reference sera panel from AAHL with the expert from AAHL. Report by: Ngo Thanh Long 5.2 Smallholder Benefits All pig and cattle and producers are potential beneficiaries. Those that take up the advice and use vaccine according to recommendations will benefit financially through reduced losses due to death and disease in their cattle and pigs. The benefits of a more profitable farming operation flow to all family members. Farmers and district veterinarians will have improved knowledge and skill in disease prevention, knowledge on the selection of the right vaccine and improving the efficacy of vaccination 5.3 Capacity Building Training and education of field veterinarians in disease prevention, disease investigation and sample collection has continued with lessons learnt from the first round of sero-surveillance and sample collection. This training has already shown an impact with an increase in quality of sample collection and number of samples collected and submitted to the laboratory. The project has provided training and technology transfer of FMD diagnostics to each laboratory involved in the project. Reagents and standard methods have been supplied to each laboratory giving them the diagnostic capability for FMDV diagnosis and serology using ELISA technology, this technology is now being practised at each laboratory. The RAHC-HCMC has virus isolation and cell culture techniques established and has begun to use molecular technology and serology by VNT using cell culture. RAHC-HCMC now has capacity to carry out RT-PCR for FMD for detection of FMD antigen and for sequence analysis. 5.4 Publicity The CARD AusAID project has received publicity through the training programs and also through the achievements so far in the project in understanding FMD in Vietnam. FMD is a disease on importance in Vietnam and this has put our project into the lime light. The project has been publicised through a press release in Australia and articles in news letters including the SEAFMD newsletter and on the internet. The results from the project have been presented at OIE/SEAFMD meetings during the project. 5.5 Project Management Implementation of the project is on schedule. AAHL has kept the project on target and has supported or requests from the Vietnamese partners. Training has been carried out in Australia and Vietnam with both partners benefiting from the information that is being generated. Implementation in Vietnam is also continuing to go well. Trainees have been supplied in a timely manner, from DAH. The field work has been organised well with data and samples being collected from farms in each pilot zone. Training and the transfer of information to other regional laboratories in Vietnam is also occurring, RAHC-HCMC is supplying support to these laboratories. 6. Report on Cross-Cutting Issues 6.1 Environment No direct environmental impacts associated with project activities have been identified. On a broader scale, the intention is that pig and cattle farming will become more efficient through a reduction in animal death and disease. Environmental benefit will result through more efficient utilization of resources such as animal feed, the energy to provide animal feed and to run other aspects of the farm. 6.2 Gender and Social Issues Application of new diagnostic tests will improve the ability of regional and provincial diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and appropriate measures to be applied to control disease. These benefits will begin during the project and continue to accrue with continued application. The beneficiaries of this project will be both large and small farms and particularly smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control. Since women at the village level are the primary animal handlers and managers, they will be major beneficiaries of the final outcome of better diagnosis and control of animal diseases. 7. Implementation & Sustainability Issues 7.1 Issues and Constraints DAH continues to spend time controlling the AI outbreaks in Vietnam and this has made there workload high. The collection of samples continues to be made more difficult by the need to train all veterinarians in each province in data collection and on how to collect samples, collection of blood and how to restrain cattle. This increased training need has highlighted the need for further training of field veterinarians throughout Vietnam in disease investigation techniques with a focus on sample collection and data collection. There is a need for further input in this area, large scale training of field veterinarians to ensure better knowledge and control of disease in Vietnam. This could be achieved though better collaboration of aid agencies in Vietnam especially those supplying training for AI. Training in data analysis by DAH staff is also an area that needs further input. 7.2 Options The government of Vietnam is looking at increasing the support to DAH and has increased funding for AI diagnosis and is looking to do the same for FMD diagnosis. The increased funding for AI has seen laboratories updated with new equipment some of which will improve all disease diagnosis which includes FMD. The money available to improve AI diagnosis can improve all disease diagnosis if this money is used wisely, e.g. in training field veterinarians in AI diagnosis, the training should be general to cover all diseases including FMD, [...]... far in the project and continue to provide support to the establishment of the technologies transferred to the laboratories The next round of the sero -surveillance for the collection of the samples will be implemented with a greater emphasis on collection of all the data on the history of the aniamals being bled In the next 6 months AAHL will finalise the transfer of technologies for FMD diagnosis and. .. support and training to field veterinarians carrying out the sero -surveillance to improve sample and data collection from the field The sero -surveillance continues to be carried out but the schedule does experience delays in collecting samples from the field to due to the AI workload The project has highlighted field veterinarians need further training in the collection of data and how to ensure the correct... correct information is obtained from the farmer The staff at RAHC-HCMC will continue to deal directly with the veterinarians in the field to ensure the correct information is collected Training of the laboratories in FMD diagnostics was completed, with a training course for 2 trainees from the RAHO-HCMC and NCVD –Hanoi laboratories held at AAHL The RAHC-HCMC continues to carry out in country training where... DAH, NaVetCo and AAHL prior to this project, is proving sustainable NAVETCO and DAH not only maintains the diagnostic capability developed at that time, but on its own initiative and unassisted by AAHL is conducting training courses and technology transfer to other labs in Vietnam 8 Next Critical Steps In the next 6 months AAHL staff will review the project outputs and to examine the information collected... consultancy visits to the recipient laboratory for support and fine tuning A useful indicator of likely success is the initiative and ability of the trainees to make a useful attempt at implementation by themselves DAH and NAVETCO staff show a high level of involvement in this way The RAHC-HCMC now has established ELISA, cell culture and molecular technologies The AusAID funded CSFV project, that linked...CSF and other diseases of importance in Vietnam Also in improving AI diagnosis in laboratories the training should emphasize how improvement can be applied to all areas of diagnosis, eg quality assurance in the laboratory 7. 3 Sustainability The DAH laboratories appear to be well supported by the central government The DAH laboratories are also receiving increased support from overseas funding agencies... trainees will visit AAHL to be trained in virus isolation and cell culture AAHL consultants will visit the laboratories to examine data from project and to review the techniques for FMD diagnosis 9 Conclusion The project activities are ahead of schedule with training and this will allow more time for the laboratories in the project to take up the technology The RAHO-HCMC are providing further support. .. agencies as part of the AI campaign to improve laboratory facilities RAHC-HCMC is gaining in confidence as an institution This confidence is beginning to be passed on to other DAH laboratories The DAH scientific staff are quick to understand new information and are energetic in its implementation People have an obvious desire to do their jobs well This desire is obvious in staff in RAHC-HCMC and other laboratories... laboratories The laboratories just need technical support to be encouraged to carry out the techniques that they need for diagnosis and research The project is following a well tried model for successful technology transfer, of demonstration and teaching of the technology in the donor facility followed by supply of reagents for implementation by the trainees at their own initiative in the recipient... returned in a quicker timeframe The introduction of molecular techniques at RAHC-HCMC has allowed sequencing results to be obtained in real time allowing DAH Vietnam to make informed decisions about serotypes of FMD circulating in Vietnam and the correct vaccine to use in each region to control FMD AAHL, DAH and WRL have cooperated on analysis of the sequence data from Vietnam isolates This project continues . Ministry of Agriculture & Rural Development Progress Report Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of. Chi Minh City continued to train and support the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals for the villages in the. of the final outcome of better diagnosis and control of animal diseases. 7. Implementation & Sustainability Issues 7. 1 Issues and Constraints DAH continues to spend time controlling the