Báo cáo nghiên cứu khoa học " Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs " pptx

Ministry of Agriculture & Rural Development Progress Report Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs Date 30/06/2006 Table of Contents Institute Information _ Project Abstract Executive Summary _ 4 Introduction & Background _ 5 Progress to Date 5.1 Implementation Highlights 5.2 Smallholder Benefits _ 5.3 Capacity Building _ 5.4 Publicity _ 5.5 Project Management _ Report on Cross-Cutting Issues _ 6.1 Environment _ 6.2 Gender and Social Issues _ Implementation & Sustainability Issues _ 7.1 Issues and Constraints _ 7.2 Options 7.3 Sustainability _ Next Critical Steps _ 10 Conclusion _ 10 10 Statuatory Declaration 13 Institute Information Project Name Vietnamese Institution Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam Vietnamese Project Team Leader Dr Dong Manh Hoa Australian Organisation Australian Personnel Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Mr Chris Morrissy Date commenced 01/06/2005 Completion date (original) 01/06/2008 Completion date (revised) Reporting period 31/12/2005 – 30/06/2006 Contact Officer(s) In Australia: Team Leader Mr Chris Morrissy Diagnostic Virologist Supervisor Mammalian Virology Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Telephone: Fax: +61 5227 5000 +61 5227 5555 Email: chris.morrissy@csiro.au Mr Chris Morrissy Name: Patents Contracts Officer Position: Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia In Vietnam Telephone: Fax: Email: +61 5227 5000 +61 5227 5555 chris.morrissy@csiro.au Dr Dong Manh Hoa Name: Director Position: Organisation Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam Telephone: Fax: Email: + 84 8568220 + 84 8569050 rahchcmc@hcm.vnn.vn Name: Position: In Australia: Administrative contact Project Abstract The projects purpose is to determine why there are vaccine failures, and to investigate what serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for these serotypes Regional laboratories are setup with the reagents and methods to allow a diagnostic capability for FMDV diagnosis and serology Control strategies for understanding of FMD epidemiology have been implemented through veterinary and laboratory training workshops and laboratory testing for both virus typing and serosurveillance has begun The project has highlighted the importance of having a laboratory network to identify what is happening in the field and how to prevent and control disease outbreaks The pilot zones are established in provinces near the borders of Vietnam to study serotypes circulating in Vietnam and to determine their origin The quality and collection of samples have increased giving more data on the FMD situation in Vietnam Molecular epidemiological studies of the FMDV isolates in these provinces will provide insights to the effectiveness of border control and the origin of FMDV circulating in Vietnam each year A diagnostic capacity for FMD would allow the early detection and identification of disease enabling better control of disease and help reduce loss of livestock and so productivity Executive Summary The main focus of this reporting period was to commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also the training of laboratory staff All laboratory equipment was purchased and supplied to the laboratories in Vietnam All laboratory consumables and reagents were also supplied to the laboratories in Vietnam for the first months of the project AAHL staff prepared the information necessary for the training of field veterinarians This workshop information and the lectures were used to run workshops for the field veterinarians in the South and Centre of Vietnam and this information was then transferred to Dr Hoa and the staff at Ho Chi Minh City Regional Laboratory who gave the third workshop in Hanoi The field studies were planned and implemented through the veterinary workshops, the field studies began with the first serosurveillance survey in the South and Centre of Vietnam and the collection of outbreak samples The Regional Animal Health Centre in Ho Chi Minh City also visited each province to train the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals on the villages in the project One staff member from the Regional Animal Health laboratory and one staff member from NAVETCO attended AAHL for training in ELISA technology for the diagnosis of FMD and standardisation of the reagents to be used in the first year of the project Two AAHL staff consultants carried out workshops for the field veterinarians in the South and Centre of Vietnam The third veterinary workshop in North Vietnam was carried out by DAH staff Two AAHL consultants also carried out a workshop for 20 participants from each of the laboratories in the project and the regional laboratories in South Vietnam, in ELISA technologies for diagnosis of FMD at the Regional Animal Health Centre in Ho Chi Minh City An important part of the laboratory training was Quality Assurance in the laboratory to ensure tests will be run according to a standard protocol and to allow AAHL to audit the results from each laboratory and give the appropriate data to allow trouble shooting Avian Influenza (AI) has extended the sero-surveillance in the first six months Introduction & Background Objectives of the project: To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies Completing these objectives will improve the diagnostic capability of the Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control This will strengthen the profile of DAH which will play a vital role in making Vietnam more economically competitive Improved animal health will lead to an increase in rural productivity though increased animal production and indirectly in increased crop production Healthy animals will enable small farmers to be more competitive in the local market Control of FMD and animal diseases in general will give poor farmers a more stable income stream and reduce their vulnerability to natural and economic problems Establishing a diagnostic network which extends from the North to South Vietnam, from the laboratory to the farm level, reinforced by training and education, will give Vietnam a working model on which to base disease control This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast Implementation Approach and Strategy The project approach is thought to be the most appropriate for developing an understanding of FMD epidemiology in Vietnam The approach for technology transfer is well established at AAHL and has been successfully applied in previous projects in Vietnam, Thailand and Indonesia The field studies and epidemiology and serosurveillance approaches have been designed and planned in conjunction with DAH to provide the maximum necessary information to demonstrate the FMD situation in Vietnam and the effectiveness of FMD vaccines The diagnostic technologies that will be used in this approach are the standard diagnostic tests in use throughout the world to study FMD as directed by OIE AAHL has a lot of experience with field surveys for prevalence of antibodies, as in the ACIAR projects in Laos and Thailand on FMD The Philippines is another example where OIE standard diagnostic tests are being used to control and eradicate FMD Progress to Date 5.1 Implementation Highlights Main achievements for this reporting period were: • Training of laboratory staff at AAHL, one representative from each laboratory were trained in ELISA techniques for the detection of FMD antigen and antibody and the standardisation of reagents Training was carried out under a quality system emphasizing the importance of Quality Assurance in the laboratory focusing on test maintenance, record keeping and data collection • A consultant from AAHL completed training of HCMC-RAHC staff in cell culture techniques and established this technology there to be used to grow FMD isolates from the field The growth of FMD isolates from the field is important to allow further analysis of FMD field isolates by PCR and sequencing, Again Quality Assurance, record keeping and data collection was emphasized • Further training of field veterinarians and feedback of the results from first round of testing and shared the experience from the first round of sample collection with the field veterinary staff to increase the quality of collected samples • A consultant from AAHL visited each laboratory to complete the transfer of ELISA technology to each laboratory and to supply the ELISA reagents Implementation of a Quality Assurance system into the laboratory and the testing of sera in each laboratory was discussed Training of staf at RAHCHCMC in the use of molecular techniques for the detection of FMD and the sequencing of FMD isolates Training of RAHC-HCMC staff in FMD serology using cell culture to carry out the Virus Neutralisation Test (VNT) • Sampling for second phase of sera collection completed for each province for epidemiological studies and serosurveillance The collection of tissue samples from outbreaks continued to increase giving valuable information on the serotypes circulating in Vietnam • ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can Tho, for serotyping of FMD tissue samples from the field Sera from first round of serosurveillance tested at RAHC-HCMC • Virus isolation in use at RAHC-HCMC Molecular technology and serology by VNT using cell culture has began to be used • Supply of consumables and further reagents for testing of samples by FMD ELISA, molecular technology and cell culture Report from Vietnam: Activities from Jan 1st 2006 to May 30th 2006 Training No Date & place 02-18 Feb 06 HCM city 30 March to 15 April 06 AAHL Subject Tissue culture ( BHK 21, BHK vacc, PK15 , BTY, Pig kidney ,Lamb kidney, Lamb testis ) Using the above cells to isolate FMDV in Vietnam and compare the sensitivity between the cells Application of ELISA for FMDV diagnosis (antigen and antibody detection , Ag-ELISA 3ABC-ELISA, LP-ELISA, CELISA ) Operation under a Quality Assurance system Reagent standardisation Person in charge Ms Catherine William Dr Long Dr.Phong Dr.Vu Remark participants Mr Chris Morrissy Ms Linda participants from partner laboratories (NVDC, RAHCHCMC, CAN THO , DA NANG ) and Sub-DAHHCMC Field activities 2.1 Visit provinces in the project (Middle and South Vietnam), March 2006 - - Transfer money for sample collection - Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples - 2.1.2 Transfer the equipment and documents (form and questionnaire) for sample collection Staff in charge: Dr Hoa, Dr Vu , Dr Ha ( RAHC-HCMC) Dr Quan, Dr.Quang (RAHC-Da Nang) Dr Thanh, Dr Dung (RAHC-Can Tho) Visit provinces in the project (North Vietnam), March 2006 - Transfer the equipment and documents (form and questionnaire) for sample collection - Transfer money for sample collection - 2.1.3 Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples Staff in charge: Dr Cam, Dr Tung (NVDC- Hanoi) Visit laboratories in the Project ( NVDC-Ha Noi , RAHC-Can tho, Da nang) early May, 2006 - Discuss the plan for second testing round and share experience on laboratory management focusing on laboratory Quality Assurance and test management 2.2 Transfer the ELISA reagents Staff in charge: Mr Chris Morrissy, Dr Long Collect sera samples from 10 provinces for the second round of surveillance, 120 cattle sera and 120 pig sera from each province - RAHC-Da Nang: provinces (Kontum and Quang Nam), total 480 sera - RAHC-Can Tho: provinces (An Giang, Kien Giang ), total 480 sera - 2.3 NVDC-Ha Noi: provinces (Quang Ninh and Lang Son), total 480 sera RAHC-HCMC: provinces (Binh Phuoc, Tay Ninh, Long An, Dong Thap), total 960 sera Collection of tissue samples from outbreaks for FMDV serotyping - NVDC-Hanoi: 208 tissue samples from North Vietnam - RAHC-Can Tho: 34 tissue samples from Mekong Delta - RAHC-HCMC : 390 tissue samples from the South Vietnam Laboratory Activities 3.1 National Vaterinary Diagnostic Center- Ha Noi (NVDC-Hanoi): - 3.2 Testing on tissue samples for FMDV serotyping: 208 samples, of them are serotype Asia and 201 of them are Serotype O Testing on sera for FMDV antibody detection: 480 sera, on going Regional Animal Health Centre Da Nang (RAHC-Da Nang) - 3.3 Testing on sera for FMDV antibody detection: 480 sera, on going Regional Animal Health Centre Can Tho (RAHC-Can Tho) - 3.4 Testing on tissue samples for FMDV serotyping : 34 samples, all of them are serotype O Testing on sera for FMDV antibody detection: 480 sera , on going Regional Animal Health Centre HCMC (RAHC-HCMC) - Testing on tissue samples for FMDV serotyping: 390 samples, of them is serotype Asia 1, the remaining are serotype O - Testing on sera for FMDV antibody detection: 960 sera , on going - Isolation FMDV by BHK cell line to confirm samples from NVDCHanoi, of them is serotype Asia1 in pigs and sample is serotype O and1 sample virus could not isolated - Isolation FMDV by BHK cell line from 39 selected field samples, was serotype Asia1 and 38 were serotype O - Application of Virus Neutralisation Test (VNT) protocol (testing phase to setup VNT technology at RAHC-HCMC): • • - Virus titration for serotype O and A , two viruses for each serotype VNT applied using the serum from vaccine trial on cattle RT-PCR application (testing phase to setup molecular technology at RAHC-HCMC, May, 2006) • • • • Extract RNA from virus serotype O, A and Asia 1, two viruses for each serotype Make cDNA from these RNA RT-PCR carried out using primers to detect serotype O and A Sent cDNA and amplicons to AAHL for sequencing Reporter: Ngo Thanh Long Note: Further detail on activities for reporting period in log frame 5.2 Smallholder Benefits All pig and cattle and producers are potential beneficiaries Those that take up the advice and use vaccine according to recommendations will benefit financially through reduced losses due to death and disease in their cattle and pigs The benefits of a more profitable farming operation flow to all family members Farmers and district veterinarians will have improved knowledge and skill in disease prevention, knowledge on the selection of the right vaccine and improving the efficacy of vaccination 5.3 Capacity Building Training and education of field veterinarians in disease prevention, disease investigation and sample collection has been carried out in the North, Centre and South of Vietnam This training has already shown an impact with an increase in quality of sample collection and number of samples collected and submitted to the laboratory The project has provided training and technology transfer of FMD diagnostics to each laboratory involved in the project Reagents and standard methods have been supplied to each laboratory giving them the diagnostic capability for FMDV diagnosis and serology using ELISA technology, this technology is now able to be practised at each laboratory 5.4 Publicity The CARD AusAID project have received publicity through the training programs and also through the achievements so far in the project in understanding FMD in Vietnam FMD is a disease on importance in Vietnam and this has put our project into the lime light The project has been publicised through a press release in Australia and articles in news letters including the SEAFMD newsletter 5.5 Project Management Implementation of the project is on schedule AAHL has kept the project on target and has supported or requests from the Vietnamese partners Training has been carried out in Australia and Vietnam with both partners benefiting from the information that is being generated Implementation in Vietnam is also continuing to go well Trainees have been supplied in a timely manner, from DAH The field work has been organised well with data and samples being collected from farms in each pilot zone Training and the transfer of information to other regional laboratories in Vietnam is also occurring RAHC-HCMC will supply support to these laboratories Report on Cross-Cutting Issues 6.1 Environment No direct environmental impacts associated with project activities have been identified On a broader scale, the intention is that pig and cattle farming will become more efficient through a reduction in animal death and disease Environmental benefit will result through more efficient utilization of resources such as animal feed, the energy to provide animal feed and to run other aspects of the farm 6.2 Gender and Social Issues Application of new diagnostic tests will improve the ability of regional and provincial diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and appropriate measures to be applied to control disease These benefits will begin during the project and continue to accrue with continued application The beneficiaries of this project will be both large and small farms and particularly smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control Since women at the village level are the primary animal handlers and managers, they will be major beneficiaries of the final outcome of better diagnosis and control of animal diseases Dr To Long Thanh 15 Dr Nguyen Thu Ha 20 Nguyen Tung, Ms 20 RAHC-DA NANG Tran Van Quan,DVM 15 Le Thanh Quang, DVM 20 Huynh Thi Manh, Ms 20 RAHC-CAN THO Nguyen Ba Thanh, Ms 15 Truong Kim Dung, Ms 20 Tran Quoc Phong, DVM 20 NAVETCO Dr.Tran Xuan Hanh 15 Nguyen Thu Hong, Ms 20 Nguyen Van Hung, Bc 20 EQUIPMENT AND OTHER SERVICES Equipment & Other Services Description Inverted Microscope Pipettes Microfuge Laptop Reagents Sample Collection Molecular technologies Laboratory consumables Freight ELIZA work Budget Limit 4000 5000 3000 4000 4000 8755 2000 4000 14000 5000 Project Progress Against Proposed Objectives, Outputs, Activities And Inputs Project Title: Vietnamese Implementing Institution: Narrative OBJECTIVES PROPOSAL Information Required To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance (Objective 1.2.2, 2.1.1 & Strategy 1) PROGRESS REPORT Performance Assumptions Information Required Measures Samples collected and Technology will be Establishment of an effective laboratory submitted to laboratory taken up by network for the diagnosis and control of FMD Data produced and results laboratories continued with training given at AAHL under a feedback to field veterinary Accurate data Quality system, method established at each staff and farms collected from farms laboratory, QA introduced and resource Little risk supplied The four DAH laboratories are working together to carry out surveillance and testing 1.1 Train field veterinary staff in outbreak investigation for the investigation of cases of FMD on farms, including record keeping, sampling strategies and data collection Samples collected from field and submitted to laboratory Sample and farm records presented with samples Accurate data collected from farms Little risk Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff 1.2 Establish ELISA technology from AAHL to collaborating laboratories Diagnostics test in use QA controls meet internal quality control sheets for examination, correct results in external proficiency testing organized by AAHL Results generated Technology will be taken up by laboratories Little risk ELISA training and reagents delivered Laboratories have began to use the ELISA technology to test samples Narrative Information Required Performance Measures Assumptions Information Required 1.3 Using cell culture at RAHCHCMC, make a collection of isolates of FMD viruses from cases of FMD disease on farms Virus isolates grown in cell culture Cell cultures can be maintained at RAHCHCMC Little risk Training at AAHL carried out and cells transferred to RAHC-HCMC FMD samples collected Further training in HCMC this months and cell culture established at RAHCHCMC 1.4 At RAHC-HCMC and AAHL use molecular techniques to assess strains of FMD circulating Sequence data produced Technology will be taken up by laboratories Little risk FMD samples collected 1.5 Introduce cell culture to NVDCHanoi for FMD diagnosis Cell culture in use at NVDC-Hanoi Technology will be taken up by laboratories Little risk Activity later in project 1.6 Firm link with AAHL for the ongoing supply of diagnostic reagents Diagnostic reagents used for routine diagnostics Reagents available Little risk Process began To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies (Objective 1.2.2, 2.1.1 & Strategy 1) Project Report presented to DAH and AusAID Feedback meetings held in provinces Project objectives meet Little risk FMD samples collected Narrative Information Required Performance Measures Assumptions Information Required 2.1 Hold information and training workshops for farmers and their veterinary advisors, with emphasis on FMD prevention and disease investigation and sample collection Samples collected from field and submitted to laboratory Sample and farm records presented with samples Accurate data collected from farms Little risk Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff Further information to farmers to be delivered later in project 2.2 Pilot zones established in each province Samples collected from field and submitted to laboratory Sample and farm records presented with samples DAH is able to have farmers agree to project Little risk Pilot zones established First round of serosurveillance completed 2.3 Feedback to collaborating laboratories results from test comparisons at AAHL and RAHC-HCMC Results on laboratory comparisons and EQA send back to each laboratory Technology will be taken up by laboratories Little risk Activity later in project 2.4 Feedback results to veterinary field staff and farmers Results maintained at field veterinary office Samples collected and tested Little risk Activity later in project 2.5 Determine the efficacy of the FMD vaccine in protecting against FMD isolates circulating in Vietnam Comparison of FMD genotypes to vaccine Results of serosurveillance Samples collected and tested Little risk Activity later in project Samples collected Hold information and training workshops farmers and their veterinary advisors, with emphasis on FMD prevention and presenting the data obtained during the CARD project 2.6 Meetings held with farmers and their veterinary advisors Project will achieve objectives Little risk Samples collected Samples collected and submitted to laboratory Data produced and results feedback to field veterinary staff and farms Technology will be taken up by laboratories Accurate data collected from farms Little risk Process began Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff Activity later in project OUTPUTS & • • • • • • • Field veterinarians that are skilled in on farm diagnosis and sample collection Education of farmers to allow better understanding of the benefits of diagnostic technologies Development of cell culture capability for virus propagation, isolation from field samples and for serology by virus neutralisation test (VNT) Diagnostic capabilities at all the laboratories participating in the project to carry out functional tests as described under a Quality System Pilot zones established and operational Firm link with AAHL for the ongoing supply of diagnostic reagents Application of technologies, knowledge and skills to other Training at AAHL carried out and cells transferred to RAHC-HCMC FMD samples collected Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource supplied Pilot zones established First round of serosurveillance completed Process began Activity later in project • 1.1-1.6 & 2.1- 2.6 • • • • diseases Increased productivity by small farm holders with more food available to poor farmers This will have a more pronounced effect on the women and children who are mainly responsible for the animals’ upkeep and likely to obtain the major benefits of increased crop production and sale of animals Field veterinarians trained in on farm diagnosis and sample collection Education of farmers to allow better understanding of the benefits of diagnostic technologies to disease control Setup farms/villages in each province for seroservalance Transfer of ELISA technology to collaborating laboratories Expected outcome at end of project Samples collected from field and submitted to laboratory Sample and farm records presented with samples Accurate data collected from farms Little risk Farmers participating in projects DAH is able to have farmers agree to project Little risk Samples collected from field and submitted to laboratory Sample and farm records presented with samples DAH is able to have farmers agree to project Little risk Diagnostics test in use QA controls met, internal quality control sheets available for examination, correct results in external Technology will be taken up by laboratories Little risk Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff Pilot zones established First round of serosurveillance completed Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource supplied proficiency testing organized by AAHL Results generated • ELISA established in each laboratory and EQA carried out in each laboratory Results on laboratory comparisons and EQA send back to each laboratory Technology will be taken up by laboratories Little risk Process began • Transfer cell culture capability to RAHC-HCMC Virus isolates grown in cell culture Cell cultures can be maintained at RAHCHCMC Little risk Training at AAHL carried out and cells transferred to RAHC-HCMC • Cell culture used to grow FMDV from field isolates FMDV isolates sequenced Cell cultures can be maintained at RAHCHCMC Little risk FMD samples collected • Transfer molecular techniques for FMDV RTPCR to RAHC-HCMC PCR products send to AAHL Technology will be taken up by laboratories Little risk Activity later in project Train RAHC-HCMC staff in sequence analysis of sequenced FMD isolates Sequence data produced PCR products submitted to AAHL Activity later in project Train NVDC in use of cell culture for FMD diagnosis Trainee at AAHL from NVDC-Hanoi Communication of diagnostic results to field veterinarians and farmers Results maintained at field veterinary offices • • • • Recommendations on efficacy of FMD vaccines Comparison of FMD genotypes to vaccine Trainee does not attend training Little risk Activity later in project Samples collected and tested Little risk Activity later in project Samples collected and tested Little risk Activity later in project • • Comparison of FMD isolates circulating in Vietnam Reagents in use in DAH laboratories and purchase of reagents by DAH for ongoing epidemiology studies and serosurveillance Results of serosurveillance Report on sequence analysis of FMD isolates FMD Isolates collected Little risk Activity later in project Diagnostic reagents used for routine diagnostics Reagents available Little risk Activity later in project • Final project report written summarising results obtained from project Meetings held with farmers and their veterinary advisors Project will achieve objectives Little risk Activity later in project • Report to DAH and results of project to be published Final reports written Project will achieve objectives Little risk Activity later in project Major activity/objective of the project, to be completed over the years of the project Diagnostic capabilities at all the laboratories participating in the project to carry out functional tests as described under a Quality System Field veterinarians that are skilled in on farm diagnosis and sample collection Education of farmers to allow better understanding of the benefits of diagnostic technologies Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource supplied ACTIVITIES & To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies Pilot zones Pilot zones established First round of serosurveillance completed established and operational Recommendations on vaccine application from epidemiological and serosurveillance data • Order and purchase equipment • Supply laboratory consumables First months year AAHL staff will prepare information and lectures for workshop for training and education of field veterinarians and farmers First months year • Run first workshop at RAHCHCMC field veterinarians First months year Workshop held at RAHC-HCMC • Supply consumables and budget for sample collection First months year Consumables and budget supplied • RAHC-HCMC to initiate epidemiological and serosurveillance programs First months year Contact made with field veterianians to begin sample collection for disease outbreaks • RAHC-HCMC and NCDCHanoi to coordinate training and meetings with field Equipment in place at laboratories First month year Consumables supplied to laboratories • First 3-6 months year Equipment in place at laboratories Consumables at laboratories Workshop held at RAHC-HCMC Workshop & meetings held Completed Information supplied and used in workshops Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff Consumables and budget supplied Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff veterinarians in provinces where fields studies are to be carried out Meetings held • • • • Hold meetings to educate farmers on the benefits of epidemiological studies, serosurveillance and laboratory diagnostics to disease control First month year Farms/village setup with information on sampling schedule and project requirement First months year Costs of sampling negotiated with farmers and field veterinary staff First months year Serosurveillance carried in each province Twice a year for years Samples collected from field and tested in each laboratory • Scientists from RAHC-HCMC laboratories to be trained in Sampling schedule documented during workshop for Veterinarians Pilot zones established First round of serosurveillance completed Document for project field studies circulated to filed veterinarians and farmers Pilot zones established First round of serosurveillance completed Payment details completed Budget for field work received and transfer to field staff Samples collected and send to laboratories Completed FMD outbreak samples tested by ELISA Activity to continue later in project Scientists trained at AAHL Completed First 3-6 months year Sampling schedule setup for each province in the project • Document produced for sampling schedule Results generated • Initial contact made with farmers in project Education to be carried out as part of National program Twice a year for years First months year FMD diagnostics, ELISAs and cell culture at AAHL First - months year Visit by AAHL consultant to RAHCHCMC Activity later in project Follow up training by AAHL to establish ELISA and cell culture techniques at RAHCHCMC • Cell cultures and consumables supplied to RAHC-HCMC Cell cultures & consumables delivered Completed First - months year Cell cultures in use Completed • Cell cultures grown and stored at RAHC-HCMC First - months year FMDV isolated from field SNT used for serology Activity later in project Workshop completed Completed • • Cell cultures used for virus isolation and serology 7-8th month of year • AAHL to run ELISA workshop at RAHC-HCMC First months year • Follow training by RAHCHCMC staff to each laboratory • FMD ELISAs established in regional laboratories • Samples send from each laboratory to RAHC-HCMC and AAHL for comparison • Accreditation of laboratory for FMD ELISAs Completed First - months year ELISA working in each laboratory Activity later in project First 6-9 months year Activity later in project Twice a year for years ELISA results send to AAHL Results of comparisons reported back to laboratories Activity later in project First 6-9 months year ELISAs functional at each laboratory, IQC available • • • • • First month year Scientists trained at AAHL Activity later in project One scientist from each of the regional laboratories trained in ELISA techniques at AAHL Visit by AAHL scientist to each regional laboratory to check ELISA operation and QA in the laboratories Visit by AAHL consultant completed Activity later in project First months year First months year EQA results collated and returned to each laboratory Activity later in project Carry out EQA testing at each laboratory Meeting to be held in Australia with project leaders to discuss progress of project, QA lectures will be part of this visit Activity later in project Activity later in project Middle of Year2 Activity later in project Carry out final round of EQA RAHC-HCMC scientists trained in molecular techniques PCR products FMD isolates in Vietnam sent to AAHL from RAHC-HCMC Second & Third year of project PCR products received at AAHL Activity later in project Sequence data from FMD isolates produced Activity later in project Third months of Year • Activity later in project First months year • Activity later in project Scientists trained at AAHL Set up external QA program for the regional laboratories at RAHC-HCMC • Sequencing on FMDV Second & Third year of isolates collected in Vietnam at AAHL project First months year AAHL staff to visit Vietnam Activity later in project AAHL staff to train regional laboratory staff in analyzing data from the field Scientist trained at AAHL Activity later in project First months year Scientist from RAHC-HCMC to be trained in cell culture and virus isolation at AAHL Scientist trained at AAHL Activity later in project First months year • Supply cells to HVDC-Hanoi Cell cultures received at HVDC-Hanoi Activity later in project First months year • Further workshops at regional centres to reinforce disease investigation protocols and to provide feedback to veterinarians and farmers on the epidemiological and serosurveillance data collected Review of project and accomplishments • Finalise results from samples from field outbreaks and report back to field veterinarians and farmers • • • One NVDC-Hanoi scientist trained at AAHL in cell culture Last months year Twice a year for years Feedback to field veterinarians and farmers on project results and recommendations Results reported to DAH and farmers Activity later in project Activity later in project Activity later in project • Finalise serology results and report back to field veterinarians and farmers Twice a year for years Results reported to DAH and farmers • Summarise results from epidemiological studies and serosurveillance from project Last months year Write report • Carry out sequencing of FMD isolates collected during project Collate & report sequence data from project Activity later in project Second & Third years of project Write recommendations for use of FMD vaccine Last months year • Draft final project report Last months year Report recommendations from project Final project meeting held Reports completed Activity later in project • Write final project reports for CARD and present report to MARD and DAH Last months year Activity later in project • INPUTS See attached statuary declaration and above for project inputs Activity later in project Activity later in project ... necessary for the training of field veterinarians This workshop information and the lectures were used to run workshops for the field veterinarians in the South and Centre of Vietnam and this information... improve the diagnostic capability of the Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control This will strengthen the profile of DAH which... commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also the training of laboratory staff All laboratory equipment was purchased and supplied