Morphological and physiological characteristics of some acid tolerant fungi species (khóa luận tốt nghiệp)

VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY  LE PHUONG THAO UNDERGRADUATE THESIS TITLE: MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERISTICS OF SOME ACID-TOLERANT FUNGI SPECIES Hanoi, March 2022 VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY  UNDERGRADUATE THESIS TITLE: MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERISTICS OF SOME ACID-TOLERANT FUNGI SPECIES Student name : Le Phuong Thao Class : K62CNSHE Faculty : Biotechnology Supervisors : Nguyen Thi Thuy Hanh, PhD Vu Nguyen Thanh, Assoc Prof PhD Hanoi, March 2022 COMMITMENT I hereby declare that: This is my study, which was conducted under the guidance of the supervisors; All data provided are true and accurate; All published data and information have been duly cited Hanoi, March 2022 Student Le Phuong Thao i ACKNOWLEDGEMENTS Firstly, I would like to express my gratitude to Assoc Prof Dr Vu Nguyen Thanh – who has given me all support, guidance, all the necessary information that made me complete the thesis He allowed me to the necessary research work and use the lab equipment needed in his lab – Center for industrial microbiology, Food Industries Research Institute In addition, I would like to extend our sincere esteems to all members in laboratory for their timely support Secondly, I owe my deep gratitude to Dr Nguyen Thi Thuy Hanh for an opportunity to conduct my thesis at Food Industries Research Institute and her invaluable guidance during the past time It is also giving my thankfulness to friends and relatives for sharing my difficulties and giving me various used advices during the process of learning and studying Finally, I would like to thanks the leaders of Vietnam National University of Agriculture, Department of Biotechnology and Food Industries Research Institute for creating conditions for me to complete my thesis well Thank you so much! Le Phuong Thao ii TABLE OF CONTENTS COMMITMENT i ACKNOWLEDGEMENTS ii TABLE OF CONTENTS iii LIST OF TABLES v LIST OF FIGURES vi ABBREVIATIONS vii ABSTRACT viii I INTRODUCTION II LITERATURE REVIEW 2.1 Introduction of acid-tolerant fungi 2.1.1 Origin and characteristics of acid-tolerant fungi 2.1.2 Some representative group of acid-tolerant fungi 2.1.3 Introduction of the genus Penicillium 2.2 Research on acid-tolerant fungi in the world and in Vietnam 11 2.2.1 Research on acid-tolerant fungi in the world 11 2.2.2 Research on acid-tolerant fungi in Vietnam 13 2.3 DNA barcoding for identification for phylogenetic species recognition 13 2.4 Methods to evaluate the biochemical and physiological characteristics of fungi 17 III MATERIALS AND METHODS 19 3.1 Materials 19 3.2 Chemicals, equipment and media 20 3.2.1 Chemicals 20 3.2.2 Equipment 20 3.2.3 Media 20 3.3 Research methods 22 iii 3.3.1 Observation of colonies, cells 22 3.3.2 Purification and maintenance of strains 22 3.3.3 Method for biochemical and physiological test 22 3.3.4 DNA extraction and purification method for fungi cells 23 3.3.5 Method to classify based on DNA barcoding 23 3.3.6 Electrophoresis method 25 3.3.7 Staining the gel and reading the result 25 IV RESULTS AND DISCUSSION 26 4.1 Observation of colonies and cells 26 4.2 Classification of strains based on rDNA sequence 28 4.3 Classification of strains based on β-tubulin and calmodulin sequence 31 4.4 The growth of strains on different environmental conditions 34 V CONCLUSION AND PROPOSAL 37 5.1 Conclusion 37 5.2 Proposal 37 REFERENCES 38 ANNEX 41 iv LIST OF TABLES Table The list of the acid-tolerant fungi, the fungi originally described as indigenous inhabitants of highly acidic habitats (pH < 3) (Hujslová et al., 2019) Table 2 Primers used for amplification and sequencing (Visagie et al., 2005) 16 Table Thermal cycle programs used for amplification (Visagie et al., 2005) 17 Table Origin of isolates of low pH acid-tolerant fungi strains 19 Table Primers used for amplification and sequencing 24 Table The colony diameter at the widest part of the colony after days of cultivation 36 v LIST OF FIGURES Figure Extreme acidic environments Figure 2 Morphological features of the Acidomyces acidophilus WKC-1 Figure Acidothrix acidophila Figure Acidea extrema Figure Microscopy of Hortaea acidophila, CBS 113389 Figure Morphological characteristics of colonies and cells on PDA medium and Malt 2Bx medium pH 1.0 of representative strains (Bar, 10 µm) 27 Figure Neighbour-joining phylogram depicting the relationships between strains and neighbouring taxa based on ITS sequences 29 Figure Neighbour-joining phylogram depicting the relationships between Amplistroma and neighbouring taxa based on D1/D2 sequences 30 Figure 4 Neighbour-joining phylogram depicting the relationships between Penicillum and neighbouring taxa based on CaM sequences 32 Figure Neighbour-joining phylogram depicting the relationships between Penicillum and neighbouring taxa based on BenA sequences 33 Figure Colonies after days of cultivation on different media 35 vi ABBREVIATIONS DNA Deoxyribonucleic Acid rDNA Recombinant Deoxyribonucleic Acid dNTPs Deoxyribonucleotide triphosphates PCR Polymerase Chain Reaction TAE Tris-acetate-EDTA ITS Internal Transcribed Spacer BenA β-tubulin CaM Calmodulin RPB2 RNA polymerase II second largest subunit vii ABSTRACT Life in natural and man-made environments with extremely low pH can be very diverse, in which microbial studies are particularly interesting It simply because the importance in biotechnology and their potential solutions to environmental pollution This study was carried out with 19 strains that have been identified with the ability to grow on low pH environment were isolated in Vietnam The results of this study have identified new species including: Amplistroma sp nov., Penicillum sp1 nov., Penicillum sp2 nov., Penicillum sp3 nov., Penicillum sp4 nov., Talaromyces sp1 nov., Sarocladium sp nov., Thyronectria sp nov The above species have also been studied in detail through DNA barcoding gene sequencing include: Internal Transcribed Spacer (ITS), β-tubulin (BenA), Calmodulin (CaM), RNA polymerase II second largest subunit (RPB2) Morphological and physiological information was also clarified by culturing the strains on different media viii Figure Neighbour-joining phylogram depicting the relationships between Penicillum and neighbouring taxa based on BenA sequences The optimal tree is shown The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site This analysis involved 32 nucleotide sequences Codon positions included were 1st+2nd+3rd+Noncoding All ambiguous positions were removed for each sequence pair (pairwise deletion option) There were a total of 381 positions in the final dataset Evolutionary analyses were conducted in MEGA11 33 Phylogenetic tree analysis based on CAM and BenA sequences has differences with ITS trees Specifically, ASM 137-2 is close to Penicillium griseolum NRRL 2671T EF506213.1 and Penicillium griseolum DTO 264-G7T KM089016.1 on BenA and CaM phylogenetic tree but close to Penicillium dravuni BPI 844248T NR 119501.1 on ITS phylogenetic tree In further study, the sequence of two RNA polymerase II subunits, the gene for elongation factor 1-α (EF1-α), will be analyzed, so that we will see more clearly the relationship and genetic differences between species of this fungi 4.4 The growth of strains on different environmental conditions To evaluate in more detail the growth and development of the strains, we cultured the strains on different media for observation, including CYA and MEA, CZ, YES, OA, CREA, DG18, MEAbl and CYAS All media are incubated at the standard temperature of 30°C for days, with additional CYA plates at 37°C that are useful to distinguish between species It is crucial that temperatures are carefully checked as small differences have a large impact on colony growth 34 Figure Colonies after days of cultivation on different media After days of cultivating the strains on different media, strains were observed the colony‟s characteristics All species that grow on YES media not generate extrolite Penicillum sp2 nov ASM 147 strain have the ability to produce acid (change medium color from purple to yellow) while other strains not Thyronectria sp nov and Sarocladium sp nov either did not grow or grew very poorly on all media In media with low water activity DG18 and CYAS, the strains on CYAS media grew worse than on DG18 media 35 Table The colony diameter at the widest part of the colony after days of cultivation Name Strain AS 175-2 Amplistroma ASS 46-1 sp nov ASS 99-1 MEAbl CREA CZ CYA CYAS DG18 F 0.5 5.8 0.9 0.35 6.3 0.4 0.3 MEA OA YES 1.8 F 0.85 1.6 2.4 0.9 F 0.5 4.5 0.9 0.35 4.9 0.8 3.5 0.5 1.4 1.4 0.4 2.05 2.3 1.1 3.2 0.4 1.7 1.3 0.4 2.2 1.6 1.15 3.4 0.5 0.3 1.9 2.1 1.2 Penicillum ASS 326-1 sp2 nov ASS 40-3 Penicillum ASS 52-1 sp2 nov ASM 115 Penicillum ASM 147 sp2 nov ASM 153-1 3.6 0.4 3.7 0.25 0.7 3.3 4.8 1.3 3.5 0.4 2.1 2.2 0.55 1.5 3.2 2.6 1.7 2.8 0.4 2.4 0.5 0.5 2.4 2.9 1.6 3.8 1.1 2.3 1.6 1.8 3.7 3.2 3.9 1.9 3.7 4.3 3.4 3.6 3.4 4.8 4.7 3.5 0.9 2.1 2.3 1.2 1.5 3.5 2.8 AS 19 Talaromyces sp1 nov ASS 348-2 3.4 0.4 1.8 2.1 1.4 2.9 2.3 1.3 3.5 0.4 2.1 2.9 0.4 1.4 2.9 3.3 ASS 135-1 Sarocladium ASS 125-1 sp nov ASS 30 Thyronectria ASS 64-1 sp nov 0.8 - 0.6 0.45 0.4 0.3 0.8 0.8 0.5 0.9 0.4 0.65 0.5 0.35 0.3 0.8 0.8 0.5 0.45 0.8 0.6 0.4 0.3 0.9 0.8 0.5 0.5 0.4 0.2 0.8 0.4 0.2 0.5 - 0.7 ASM 139-1 Penicillum ASM 141-1 sp1 nov ASM 137-2 Note: F: Full plate; Unit (Cm ±0,1) 36 V CONCLUSION AND PROPOSAL 5.1 Conclusion From 19 strains used for research, new species have been identified, including: Amplistroma sp nov (AS 175-2, ASS 46-1, ASS 99-1), Penicillium sp1 nov (ASS 137-2, ASM 139-1, ASM 141-1), Penicillium sp2 nov (ASS 40-3, ASS 326-1), Penicillium sp3 nov (ASS 52-1), Penicillium sp4 nov (ASM 115, ASM 147, ASM 153-1), Talaromyces sp1 nov (AS 616-3, AS 19, AS 348-2), Sarocladium sp nov (ASS 135-1, ASS 125-1, ASS 30), Thyronectria sp nov (ASS 64-1) Based on the ITS, CAM, and BEN sequences, the phylogenetic tree has been built and the preliminary taxonomic positions of the above species have been identified Based on the growth of species on different environments, some physiological characteristics of species have been identified 5.2 Proposal It is necessary to continue to study other barcode genes such as RPB2, EF1- α of species to determine the exact taxonomic position Continue to describe in detail the morphological characteristics of the new species 37 REFERENCES Aguilera, A et al (2007) „Distribution and seasonal variability in the benthic eukaryotic community of Rio Tinto (SW, Spain), an acidic, high metal extreme environment‟, Systematic and Applied Microbiology, 30(7), pp 531–546 Aguilera, A and González-Toril, E (2019) „Eukaryotic life in extreme environments: acidophilic fungi‟, Fungi in extreme environments: ecological role and biotechnological significance, pp 21–38 Amaral Zettler, L A et al (2002) „Eukaryotic diversity in Spain‟s River of Fire‟, Nature, 417(6885), p 137 Baker, B J et al (2004) „Metabolically active eukaryotic communities in extremely acidic mine drainage‟, Applied and environmental microbiology, 70(10), pp 6264–6271 Brake, S S and Hasiotis, S T (2010) „Eukaryote-dominated biofilms and their significance in acidic environments‟, Geomicrobiology Journal, 27(6–7), pp 534–558 Chan, W K et al (2019) „Acidomyces acidophilus: Ecology, biochemical properties and application to bioremediation‟, in Fungi in extreme environments: ecological role and biotechnological significance Springer, pp 505–515 Chen, A J et al (2013) „Identification of ochratoxin A producing fungi associated with fresh and dry liquorice‟, PLoS One, 8(10), p e78285 Frisvad, J C and Samson, R A (2004) „Polyphasic taxonomy of Penicillium subgenus Penicillium A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins‟, Studies in mycology, 49(1), pp 1–174 Giraud, F et al (2010) „Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing‟, International journal of food microbiology, 137(2–3), pp 204–213 10 Glass, N L and Donaldson, G C (1995) „Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes‟, Applied and environmental microbiology, 61(4), pp 1323–1330 11 González-Toril, E et al (2015) „Pyrosequencing-based assessment of the microbial community structure of Pastoruri glacier area (Huascarán National Park, Perú), a natural extreme acidic environment‟, Microbial ecology, 70(4), pp 936–947 12 Hölker, U et al (2004) „Hortaea acidophila, a new acid-tolerant black yeast from lignite‟, Antonie van Leeuwenhoek, 86(4), pp 287–294 13 Houbraken, J et al (2012) „New penicillin-producing Penicillium species and an overview of section Chrysogena‟, Persoonia-Molecular Phylogeny and Evolution of Fungi, 29(1), pp 78–100 14 Houbraken, J and Samson, R (2011) „Phylogeny of Penicillium and the segregation of Trichocomaceae into three families‟, Studies in mycology, 70(1), pp 1–51 15 Huhndorf, S M et al (2009) „Amplistroma gen nov and its relation to Wallrothiella, two genera with globose ascospores and acrodontium-like anamorphs‟, Mycologia, 101(6), pp 904–919 16 Hujslová, M et al (2014) „Three new genera of fungi from extremely acidic soils‟, 38 Mycological progress, 13(3), pp 819–831 17 Hujslová, M et al (2019) „Fungi, a neglected component of acidophilic biofilms: they have a potential for biotechnology?‟, Extremophiles, 23(3), pp 267–275 18 Kambura, A K et al (2016) „Bacteria and Archaea diversity within the hot springs of Lake Magadi and Little Magadi in Kenya‟, BMC microbiology, 16(1), pp 1–12 19 Krüger, A et al (2018) „Towards a sustainable biobased industry–highlighting the impact of extremophiles‟, New Biotechnology, 40, pp 144–153 20 Peterson, S W et al (2005) „Penicillium coffeae, a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P fellutanum, P thiersii and P brocae based on parsimony analysis of multilocus DNA sequences‟, Mycologia, 97(3), pp 659– 666 21 Peterson, S W and Jurjević, Ž (2013) „Talaromyces columbinus sp nov., and genealogical concordance analysis in Talaromyces clade 2a‟, PloS one, 8(10), p e78084 22 Rothschild, L J and Mancinelli, R L (2001) „Life in extreme environments‟, Nature, 409(6823), pp 1092–1101 23 Schoch, C L et al (2012) „Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi‟, Proceedings of the National Academy of Sciences, 109(16), pp 6241–6246 24 Schoch, C L et al (2014) „Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi‟, Database, 2014 25 Schulze-Makuch, D., Airo, A and Schirmack, J (2017) „The adaptability of life on earth and the diversity of planetary habitats‟, Frontiers in microbiology, 8, p 2011 26 Sigler, L and Carmichael, J W (1974) „A new acidophilic Scytalidium‟, Canadian Journal of Microbiology, 20(2), pp 267–268 27 Skorupa, D J et al (2013) „Cyanidiales diversity in Yellowstone national park‟, Letters in applied microbiology, 57(5), pp 459–466 28 Sletten, O and Skinner, C E (1948) „Fungi capable of growing in strongly acid media and in concentrated copper sulfate solutions‟, Journal of Bacteriology, 56(5), pp 679– 681 29 Starkey, R L and Waksman, S A (1943) „Fungi tolerant to extreme acidity and high concentrations of copper sulfate‟, Journal of Bacteriology, 45(5), pp 509–519 30 Stoeck, T., Przybos, E and Dunthorn, M (2014) „The D 1‐ D region of the large subunit ribosomal DNA as barcode for ciliates‟, Molecular Ecology Resources, 14(3), pp 458–468 31 Thanh, V N et al (2019) „Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity Sci Rep 9: 3674‟ 32 Tiquia-Arashiro, S and Rodrigues, D F (2016) Extremophiles: applications in nanotechnology Springer 33 Visagie, C M et al (2005) „Identification and nomenclature of the genus Penicillium‟, Studies in mycology, 53(1), pp 53–62 34 Visagie, C M et al (2014) „Aspergillus, Penicillium and Talaromyces isolated from 39 house dust samples collected around the world‟, Studies in Mycology, 78, pp 63–139 35 Wang, B and Wang, L (2013) „Penicillium kongii, a new terverticillate species isolated from plant leaves in China‟, Mycologia, 105(6), pp 1547–1554 36 White, T J et al (1990) „Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics‟, PCR protocols: a guide to methods and applications, 18(1), pp 315–322 37 Yuan, P et al (2011) „A novel acidic and low-temperature-active endo-polygalacturonase from Penicillium sp CGMCC 1669 with potential for application in apple juice clarification‟, Food Chemistry, 129(4), pp 1369–1375 40 ANNEX Annex Sequences AS175-2 ITS5-NL4 Amplistroma erinaceum CBS 134881 NR_145379.1 97.28% ATACCGCGTACCTAGCCGGATAACATATGCATCTGCCTCGGCGGCGGCCCCAGGGGGGGCCTCTGCCCGCCAAGC CGCAACCCGCGGTACGGCGGGCGCCGCCGGTGGCGCCTAAACTAAAAAGCATTGTAAGTGGGTTATACCACTGCA AAAGAACAAAACAAAAGATAAAAATCAAAACCCATTACAACCTGCAACAACGGATCTCTTGGCTCCGGCATCGAT GAAGAACGCAGCGAAATGCGATACGTAATGCGAATTGCAGATCTCCAGTGAGTCATCGAATCTTTGAACGCACAT AGCGCCCGCCAGCTCTCTGGCGGGCATGCCCGTCCGAGCGTCGATTACACCGCTCGAGCGCGCCTCTCTACCCGG GAGGTCGTCGCTCGGTGTTGGGGCAGTAGGGGGGATTTCTCTCCCCTACGCCCCCAAAAGCATTGCGCGGCCCTC GCATCAGGACCCAAGCACAGTAAACTTCTCACGTTGAGCTGCTTAGGGACACCTACGCCGAGGCGCCAGCCGTAA AACCCCCAACATAGGCCTGCTCGCCAGGCCGCCTGATGTTGACCTCGGATCGGGTAGGACGACCCGCTGAACTTA AGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAGCGGCGAGCGAAGCGGCAAAAGCTCA GATTTGAAATCTGGCCGTCCCCGCGGCGGCCCGAATTGTAATCTGTAGGGGATGGTTCCAACTGACGTGTTCTGC CTAAGTCCCCTGGAACGGGGCGCCATAGAGGGTGAGAGCCCCGTGAGGTAGGGCGCGCTCGGTAAATTGTGAATC TCCTCCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATTCCTTCTAAAGCTAAATACCGGC CGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGGTGAAAAGCACTTTGGAAAGAGAGTGAAATAGTACGTGA AATTGTTGCAGGGGAAGCGCTCTTGACCAGTCGCGCGCCGTGGGATCAGCCGTCGTTTACTCGGCGGTGTACTCC CTCCGGTTGCGGGTCAGCACCGGCTCTGGCGGTGGGATAGGCTCGCTGGGAACGTGGCCCTGCCTTCGGGTGGGG TGTTATAGCCCCGGTGTGCGTACCGCCTGCTGGGGCCGAGGT ASS46-1 ITS5-ITS4 Amplistroma erinaceum CBS 134881 NR_145379.1 97.2% TTGATATGCTTAAGTTCAGCGGGTCGTCCTACCCGATCCGAGGTCAACATCAGGCGGCCTGGCGAGCAGGCCTAT GTTGGGGGTTTTACGGCTGGCGCCTCGGCGTAGGTGTCCCTAAGCAGCTCAACGTGAGAAGTTTACTGTGCTTGG GTCCTGATGCGAGGGCCGCGCAATGCTTTTGGGGGCGTAGGGGAGAGAAATCCCCCCCCTACTGCCCCAACACCG AGCGACGACCTCCCGGGTAGAGAGGCGCGCTCGAGCGGTGTAATCGACGCTCGGACGGGCATGCCCGCCAGAGAG CTGGCGGGCGCTATGTGCGTTCAAAGATTCGATGACTCACTGGAGATCTGCAATTCGCATTACGTATCGCATTTC GCTGCGTTCTTCATCGATGCCGGAGCCAAGAGATCCGTTGTTGCAGGTTGTAATGGGTTTTGATTTTTATCTTTT GTTTTGTTCTTTTGCAGTGGTATAACCCACTTACAATGCTTTTTAGTTTAGGCGCCACCGGCGGCGCCCGCCGTA CCGCGGGTTGCGGCTTGGCGGGCAGAGGCCCCCCCTGGGGCCGCCGCCGAGGCAGATGCATATGTTATCCGGCTA GGTACGCGGTATGGTTAAGAACAGTAGGTGGTCTGTAGACCCTCTAATGATCCCTCCGCTGGTTC ASS99-1 ITS1F-ITS4 Amplistroma erinaceum CBS 134881 NR_145379.1 97.27% ATATGCTTAAGTTCAGCGGGTCGTCCTACCCGATCCGAGGTCAACATCAGGCGGCCTGGCGAGCAGGCCTATGTT GGGGGTTTTACGGCTGGCGCCTCGGCGTAGGTGTCCCTAAGCAGCTCAACGTGAGAAGTTTACTGTGCTTGGGTC CTGATGCGAGGGCCGCGCAATGCTTTTGGGGGCGTAGGGGAGAGAAATCCCCCCCCTACTGCCCCAACACCGAGC GACGACCTCCCGGGTAGAGAGGCGCGCTCGAGCGGTGTAATCGACGCTCGGACGGGCATGCCCGCCAGAGAGCTG GCGGGCGCTATGTGCGTTCAAAGATTCGATGACTCACTGGAGATCTGCAATTCGCATTACGTATCGCATTTCGCT 41 GCGTTCTTCATCGATGCCGGAGCCAAGAGATCCGTTGTTGCAGGTTGTAATGGGTTTTGATTTTTATCTTTTGTT TTGTTCTTTTGCAGTGGTATAACCCACTTACAATGCTTTTTAGTTTAGGCGCCACCGGCGGCGCCCGCCGTACCG CGGGTTGCGGCTTGGCGGGCAGAGGCCCCCCCTGGGGCCGCCGCCGAGGCAGATGCATATGTTATCCGGCTAGGT ACGCGGTATGGTTAAGAACAGTAGGTGGTCTGTAGACCCTCTAATGATCCCTCCGCTGGTTCACCAACGGAGACC TTG ASM137-2 ITS5-ITS4 Penicillium chalabudae CBS 219.66 NR_144845.1 95.48% TTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAACCTGAAAAAAAGGATGATTTGTCGGCAGGCGCCGGCC GGGCCTACGGAGCGGGTGACAAAGCCCCATACGCTCGAGGACCGGACGCGGTGCCGCCGCTGCCTTTCGGGCCCG TCCCCCGGGGAGTCGGGGGACGAGGGCCCAACACACAAGCCGTGCTTGAGGGCAGCAATGACGCTCGGACAGGCA TGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCACTGAATTCTGCAATTCACATTA TTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTTTAACTAATTTAG CTAATTGCTCAGACTGCATTATTCAGACAGCGTTCAATGGTGTCTTCGGCGGGCGCGGGCCCGGGGGCGGATGCC CCCCGGCGGCCATGACGGCGGGCCCGCCGAAGCAACAAGGTACGATAAACACGGGTGGGAGGTTGGACCCAGAGG GCCCTCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTG ASM 139-1 Penicillium dravuni BPI 844248 NR_119501.1 97.8% AAAGGATGATTTGTCGGCAGGCGCCGGCCGGGCCTACGGAGCGGGTGACAAAGCCCCATACGCTCGAGGACCGGA CGCGGTGCCGCCGCTGCCTTTCGGGCCCGTCCCCCGGGGAGTCGGGGGACGAGGGCCCAACACACAAGCCGTGCT TGAGGGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGA TGATTCACTGAATTCTGCAATTCACATTATTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGA TCCGTTGTTGAAAGTTTTAACTAATTTAGCTAATTGCTCAGACTGCATTATTCAGACAGCGTTCAATGGTGTCTT CGGCGGGCGCGGGCCCGGGGGCGGATGCCCCCCGGCGGCCATGACGGCGGGCCCGCCGAAGCAACAAGGTACGAT AAACACGGGTGGGGAGGTTGGACCCAGAGGGCCCTCACTCAGTAATGATCCTTCCGCAGGTTCCCCTACGGAAGG ATCATTACTGAGTGAGGGCCCTCTGGGTCCAACCTCCCACGCGTGTTTATCGTACCTTGTTGCTTCGGCGGGCGC GCCGTCATGGCCGCCGGGGGGCATCCGCGCTCGGTCTCGCGCCCGCCGAAGACACCATTGAACGCTGTCTGAATA ATGCAGTCTGAGCAATTAGCTAAATTAGTTAAGACTTTCAACGACGGATCTCTTGGTTCCGGCATCGATGAAGAA CGCAGCGAAAT ASM141-1 ITS4 Penicillium dravuni BPI 844248 ITS NR_119501.1 97.96% GCGCCGGCCGGGCCTACGGAGCGGGTGACAAAGCCCCATACGCTCGAGGACCGGACGCGGTGCCGCCGCTGCCTT TCGGGCCCGTCCCCCGGGGAGTCGGGGGACGAGGGCCCAACACACAAGCCGTGCTTGAGGGCAGCAATGACGCTC GGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCACTGAATTCTGCAA TTCACATTATTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTTTAA CTAATTTAGCTAATTGCTCAGACTGCATTATTCAGACAGCGTTCAATGGTGTCTTCGGCGGGCGCGGGCCCGGGG GCGGATGCCCCCCGGCGGCCATGACGGCGGGCCCGCCGAAGCAACAAGGTACGATAAACACGGGTGGGAGGTTGG ACCCAGAGGGCCCTCACTCATTAATGATCCTTCCGCAGGTTCACCTACGGAAGGATCATTACTGAGTGAGGGCCC TCTGGGTCCAACCTCCCACCCGTGTTTATCGTACCTTGTTGCTTCGGCGGGCCCGCCGTCATGGCCGCCGGGGGG CATCCGCCCCCGGGCCCGCGCTCGCTGAAGACACCATTGAACGCTGTCTGAATAATGCAGTCTGAGCAATTAGCT AAATTAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAATA 42 ATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATG CCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCTCGTTCCCCGACTCCCGGGGGACGG GCCCGAAAGGCAGGGGGGGACCGCGTCGGGTCTCGGGCGTTGGGGCTTTGTNCCCGCCCCGTAGCCCGGCCGGGG CCTGC ASS40-3 ITS1-NL4 Thermoascus crustaceus CBS 181.67 NR_144915.1 87.8% TACCATACCCTGTTGCTTCGGCGGGCCCGCCCTCAGCGGCCGCCGGGATGGCCCCTTGCGCCTCCGGGCCCGCGC CCGCCGAAGACACCTGTGAACGCTTCTATTGAAGATTGCAGTCTGAGTGATTTTTTTCCAAATCAATCAAAACTT TCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAAT TCAGTGAATCATCGAGTCTTCGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCAT TGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCTCCTTCCCGGGGGGGACGGGCCCGAAAGGCAGC GGCGACTTCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCACTCCGCTCTGGTAGGAACGGCCGACAGTCTGCC GACTCTCCAACCTTCTTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATA AGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAATCT GGCCCCTCCGGGGTCCGAGTTGTAATTTGCAGAGGATGCTTCGGGTGCGGCCCCTATCTAAGTGCCCTGGAACGG GCCGTCATAGAGGGTGAGAATCCCGTCTGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGT TGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGGAGACCGATAGCGCAC AAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGC GCTTGCGACCAGACTCGCCTGCGGGGTTCAGCCGGCCTTCGGGCCGGTGTACTTCCCCGCAGGCGGGCCAGCGTC GGTTTGGGCGGCCGGTCAAAGGCCTCCGGAATGTGTCGCCCTTCGGGGCGTCTTATAGCCGGAGGTGCAATGCGG CCAGCCTGGACCGAG ASS326-1 ITS4 Penicillium chermesinum NRRL 2048 NR_121310.1 99.8% CTGAAAAAAGGGGTTGAGGGGGTCGGCTGGCGCCGGCCGGGCCTGCGGAGCGGGTGACAGAGCCCCATACGCTCG AGGACCGGACGCGGTGCCGCCGCTGCCTTTCGGGCCCGTCCCCGGGGGGACGGCGCCCAACACACAAGCCGGGCT TGAGGGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGA TGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGA TCCGTTGTTGAAAGTTTTAACTAATTTATGCGTATCGCTCAGACTGCAATCTTCAGACAGCGTTCAATGATGTCT TCGGCGGGCGCGGGCCCGGGGGCAGGTGCCCCCCGGCGGCCAGGCTGGCGGGCCCGCCGAAGCAACAAGGTACGG TATACACGGGTGGGAGGTTGGACCCAGAGGGCCCGCACTCAGTAATGATCCTTCCGCAGGT ASS52-1 ITS5-ITS4 Talaromyces thailandensis CBS 133147 NR_147428.1 91.36% TTGATATGCTTAAGTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCGTGGAAAAATGATGGAAGACCAACG CCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGACCTAGGCGGGCGTCGCCACTGCCTTTC AGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCGGACAGGCATG CCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACT TATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTGATTATTATCAAT CATGCTCAGACAGTCCATCTTCATGAGGGTTCAAGGGCGCTCTGGCGGGCACGGGCCCAGGGGCACAGGGGCTCC TCGGCGACCGGGAGAGCCCAGTGGGCCCGCCAAAGCAACAGGGTGTACGAGAGACAAGGGTGGGAGGTTCGGTCC ACGCGGGACCGGCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACG 43 ASM115 ITS5-ITS4 Talaromyces albisclerotius CBS 141839 NR_170776.1 92.1% ATGCTTAAGTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCGTGGGAATAAATTCGTGAGGTGACCAACGC TCCGCGGGTCCATCCCGAGCGAGTGACAAAGCCCCATACGCTCGAGGACCTGGGCGGGAACGTCGCCGCTGCCTT TCGGGCAGGTCCCCCCGTTTCGGAGGGACCAGCGCCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCG GACAGGCATGCTCCCCGGAATGCCAGGGAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAAT TCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTGAT TATTATCAACGTTGCTCAGACAGTCCATCTTCACAAAGGGTTCAAGGGGCGCTCCGGCGGGCACGGGCCCGAGGA CAGAGGCGTCCTCCGGCGACCGGGAGACCCGGTGGGCCCGCCAAAGCAACACGGTATTCAAATAGACACGGGTGG GAGGTTGGACCGCGAGGGGTCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGT ASM147 ITS5-ITS4 Talaromyces albisclerotius CBS 141839 NR_170776.1 92.12% ATGCTTAAGTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCGTGGAATAAATTCGTGAGGTGACCAACGCT CCGCGGGTCCATCCCGAGCGAGTGACAAAGCCCCATACGCTCGAGGACCTGGGCGGGAACGTCGCCGCTGCCTTT CGGGCAGGTCCCCCCGTTTCGGAGGGACCAGCGCCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCGG ACAGGCATGCTCCCCGGAATGCCAGGGAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATT CACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTGATT ATTATCAACGTTGCTCAGACAGTCCATCTTCACAAAGGGTTCAAGGGGCGCTCCGGCGGGCACGGGCCCGAGGAC AGAGGCGTCCTCCGGCGACCGGGAGACCCGGTGGGCCCGCCAAAGCAACACGGTATTCAAATAGACACGGGTGGG AGGTTGGACCGCGAGGGGTCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACG ASM153-1 ITS5-ITS4 Talaromyces albisclerotius CBS 141839 NR_170776.1 92.1% ATGCTTAAGTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCGTGGGAATAAATTCGTGAGGTGACCAACGC TCCGCGGGTCCATCCCGAGCGAGTGACAAAGCCCCATACGCTCGAGGACCTGGGCGGGAACGTCGCCGCTGCCTT TCGGGCAGGTCCCCCCGTTTCGGAGGGACCAGCGCCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCG GACAGGCATGCTCCCCGGAATGCCAGGGAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAAT TCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTGAT TATTATCAACGTTGCTCAGACAGTCCATCTTCACAAAGGGTTCAAGGGGCGCTCCGGCGGGCACGGGCCCGAGGA CAGAGGCGTCCTCCGGCGACCGGGAGACCCGGTGGGCCCGCCAAAGCAACACGGTATTCAAATAGACACGGGTGG GAGGTTGGACCGCGAGGGGTCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGT AS19 ITS1-ITS1F-ITS4 Thermoascus crustaceus CBS 181.67 NR_144915.1 87.16% AGCATCCTCTCCAAATTACAACTCGGACCCCGACGGGGCCAGATTTCAAATTTGAGCTCTTGCCGCTTCACTCGC CGTTACTGAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAACTCCTAC CTGATCCGAGGTCAACCGTGGGAAAAAAGATTGAGGTGATCGACCAAGCGCCCGCAGGCCCCTACAGAGCGAGTG ACAAAGCCCCATACGCTCGAGGACCTAGGACGCGGCGCCGCCGCTGCCTTTCGGGCACGTCCCCCCCGGGAAGGA GGACGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATA CCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG CTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTAAGTTATTTAACTAATTGACACTCA GACAGTCAATCTTCAGACAGCGTTCACATGAGCGTCTCTCGGCGGGCGCGGGCCAGAGGGCGAACACGCCCCCCG 44 GCGACCGGGGTGGCCCCGGCGGGCCCGCCAAAGCAACAAGGTATACGATAGAGACACGGGTGGGAGGTTGGACCC AGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC ASS348-2 ITS4 Penicillium georgiense NRRL 35509 NR_121325.1 97.61% CCGAGGTCAACATAAGAAGTTGTGCTTGAAGGGCAAGCGACCGGCCGGCCTACAAAGCGGGTGACAAAGCCCCAT ACGCTCGAGGACCGGACGCGGTGCCGCCATTGCATTTGAGGCCTGTCCCCCCGGAACGGAGGGACATAACCCAAG GACAAGCCGGGCTTGAGGGGTGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCG TTCAAAGACTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGC CGGAACCAAGAGATCCGTTGTTGAAAGTTTTAACTGATTAAAATTCCACTCAGACAACACTCACAGACAGAGTTC CAGGGTGTCTTCGGCGGGCGCACGCCCGGGGACAGCGTCCCCCGGCGGCCTGACAGCAGGCGGGCCCACCGAAGC AACAAGGTACAATAGACACGGGTGGGAGGTTAGACCCAGAGGGCCTGCACTCATTAATGATCCTTCCGCAG AS616-3 ITS1-ITS4 Penicillium georgiense NRRL 35509 NR_121325.1 97.61% AGGAGCTTCACACGGGCGCGGCCGCCCATCCCAGACGGGATTCTCACCCTCTATGACGGCCCGTTCCAGGGCACT TAGACGGGGACCGCACCCGAAGCATCCTCTCCAAATTACAACTCGGACCCCGACGGGGCCAGATTTCAAATTTGA GCTCTTGCCGCTTCACTCGCCGTTACTGAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAA GTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCGTGGAAAAATGTTGAGGGATGACCAACGCCCGCAGGCC CCTCCCGAGCGGGTGACAAAGCCCCATACGCTCGAGGACCTAGGCGGGCGTCGCCGCTGCCTTTCGGGCAGGTCC CCCCCGGGAAGGGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGCTCGGACAGGCATG CCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACT TATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTTGATTATTATCAAG ACACTCAGACAGTCCATCTTCATAAGGGTTCACAGAGCGCTCTGGCGGGCGCGGGCCCGGGGGCGAACACGCCCC CCGGCGACCGGGGTGGCCCCGGTGGGCCCGCCAAAGCAACAGGGTATACAAGAGACACGGGTGGGAGGTTGGACC CGCGAGGGGTCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCCTACGG ASS135-1 NL4-ITS5 Hypomyces peltigericola CBS 141848 NR_148180.1 88.85% CTCAGCCCGCCCCAGGGTATTGCGCAACGGTCTATAACACTCCCGAGGGAGCCACATTCCCGAAGCCTTTATCCC CCGCGGCGAGCTGATGCTGGCCTGGACCGGCGGAGTGCGCCGGCGAGAACGCCGGCTGATCCGCCAGGCCCAAGT CTGGTCACGAGCGCTTCCCTTTCAACAATTTCACGTACTGTTTAACCCTCTTTTCAAAGTGCTTTTCATCTTTCG ATCACTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGACATATACCTCCCATTTTGAGCAG CATTCCCAAACTACTCGACTCGTCGAAGGAGCTTTACAGAGGCTAGGCATCCGACCGGACGGGGCTCTCACCCTC TGTGGCGTCCCGTTCCAGGGAACTCGGAAGGCGCCGCGCCAAAAGCATCCTCTGCAAATTACAACTCGGACCCGC GGGGGGCCAGATTTCAAATTTGAGCTGTTGCCGCTTCACTCGCCGTTACTGGGGCAATCCCTGTTGGTTTCTTTT CCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTACTCCTGCCTGATCCGAGGTCAACCTCGTGAAAATGGTTCG TTGCGTCCTGGTGGACGGCGTGGCCGCCGCCGCGTCCCGGTGCGAGGTGCTTGCTACTACGCAGGGGGAGGCGCG GGCGGGGCCGCCACTGGATTTCGGGGACGGCGGGGCCGGAGCTGGAGCCGTTCCCCAACGCCAGGCACCCCCCCC GCCCCGGTGAAGGGGAGGAGGAGGCCTGAGGGTCGAAATGACGCTCGAACAGGCATGCCCGCCAGAGTGCTGGCG GGCGCAATGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGT TCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTTTGATTTATTCGTGGAGACTCAGAAAAGACACT 45 GCGATAAAGCCACGAGTTTCGGGCCCCCGGCGGCCGCGCGGGCGCGCCGAGGCAACGACGGGTACGGTTCACAGG GGGTTTTGGG ASS125-1 Contig ITS1-ITS4 Hypomyces peltigericola CBS 141848 NR_148180.1 88.85% GTTGCCTCGGCGCGCCCGCGCGGCCGCCGGGGGCCCGAAACTCGTGGCTTTATCGCAGTGTCTTTTCTGAGTCTC CACGAATAAATCAAAACTTTCAACAACGGATCTCTTGGCTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAA GTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCACTCTGGCGGGC ATGCCTGTTCGAGCGTCATTTCGACCCTCAGGCCTCCTCCTCCCCTTCACCGGGGCGGGGGGGGTGCCTGGCGTT GGGGAACGGCTCCAGCTCCGGCCCCGCCGTCCCCGAAATCCAGTGGCGGCCCCGCCCGCGCCTCCCCCTGCGTAG TAGCAAGCACCTCGCACCGGGACGCGGCGGCGGCCACGCCGTCCACCAGGACGCAACGAACCATTTTCACGAGGT TGACCTCGGATCAGGCAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGAT TGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCCCGCGGGTCCGAGTTGTAATT TGCAGAGGATGCTTTTGGCGCGGCGCCTTCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCC GGTCGGATGCCTAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTA TATGTCTTCTAAAGCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTT TGAAAAGAGGGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGC ASS30 ITS1-ITS4 Hypomyces peltigericola CBS 141848 NR_148180.1 89.14% GAACCTCAGCCCGCCCCAGGGTATTGCGCAACGGTCTATAACACTCCCGAGGGAGCCACATTCCCGAAGCCTTTA TCCCCCGCGGCGAGCTGATGCTGGCCTGGACCGGCGGAGTGCGCCGGCGAGAACGCCGGCTGATCCGCCAGGCCC AAGTCTGGTCACGAGCGCTTCCCTTTCAACAATTTCACGTACTGTTTAACCCTCTTTTCAAAGTGCTTTTCATCT TTCGATCACTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGACATATACCTCCCATTTTGA GCAGCATTCCCAAACTACTCGACTCGTCGAAGGAGCTTTACAGAGGCTAGGCATCCGACCGGACGGGGCTCTCAC CCTCTGTGGCGTCCCGTTCCAGGGAACTCGGAAGGCGCCGCGCCAAAAGCATCCTCTGCAAATTACAACTCGGAC CCGCGGGGGGCCAGATTTCAAATTTGAGCTGTTGCCGCTTCACTCGCCGTTACTGGGGCAATCCCTGTTGGTTTC TTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTACTCCTGCCTGATCCGAGGTCAACCTCGTGAAAATGG TTCGTTGCGTCCTGGTGGACGGCGTGGCCGCCGCCGCGTCCCGGTGCGAGGTGCTTGCTACTACGCAGGGGGAGG CGCGGGCGGGGCCGCCACTGGATTTCGGGGACGGCGGGGCCGGAGCTGGAGCCGTTCCCCAACGCCAGGCACCCC CCCGCCCCGGTGAAGGGGAGGAGGAGGCCTGAGGGTCGAAATGACGCTCGAACAGGCATGCCCGCCAGAGTGCTG GCGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTG CGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTTTGATTTATTCGTGGAGACTCAGAAAAGAC ACTGCGATAAAGCCACGAGTTTCGGGCCCCCGGCGGCCGCGCGGGCGCGCCGAGGCAACGACGGGTACGGTTCAC AGGGGGTTTTGGGAGTTGTGACACTCTGTAATGATCCCTCCGCAGGTTCACCTACGGAGGGATCATTACAGAGTG TCACAACTCCCAAAACCCCCTGTGAACCGTACCCGTCGTTGCCTCGGCGCGCCCGCGCGGCCGCCGGGGGCCCGA AACTCGTGGCTTTATCGCAGTGTCTTTTCTGAGTCTCCACGAATAAATCAAAACTTTCAACAACGGATCTCTTGG CTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCT TTGAACGCACATTGCGCCCGCCAGCACTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCGACTCTCAGGCCTCCT CCTCCCCTTCACCGGGGCGGGGGGGTGCCTGGCGTTGGGGAACGGCTCCAGCTCGGGCCCCGCCGTCCCCGAAAT CCAGTGGCGGCCCCGCCCGCGCTTCCCCTGCGTAGTAGC ASS64-1 ITS1F-NL4-ITS4 46 Stachybotrys limonispora CBS 128809 NR_156604.1 85.59% GCGCGAACCTCAGTCCGCCCCAGGGTATTACGCAACGGGCTATAACACTCCCGAGGGAGCCACATTCCCGAAACC TTTATCCCCCGGCGCGAACTGATGCTGGCCTGGACAGGCAAAGTACCCCGGCGAGAACGCCGGGTGATTCACCAC GCCCAAGTCTGGTCACAAGCGCTTCCCTTTCAACAATTTCACGTACTATTTAACCCTCTTTTCAAAGTGCTTTTC ATCTTTCGATCACTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGACATATACCTCCCATT TAGAGCAGCATTCCCAAACTACTCGACTCGTCGAAGGAGCTTTACAGAGGCTAGGTATCCAACCAGACGGGGCTC TCACCCTCTATGGCGTCCCGTTCCAGGGAACTCGGAAGGCACCGCACCAAAAGCATCCTCTGCAAATTACAACTC GGGCCTGGGGCCAGATTTCAAATTTGAGCTGTTGCCGCTTCACTCGCCGTTACTGGGGCAATCCCTGTTGGTTTC TTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATTCCTACCTGATCCGAGGTCAACATTCAGAAAAAAA TGATTTAGTTTACGGCGTGGCCGCGCCGCGTTCCAGTGCGAGGTGGTTTACTACTACGCAAGAGACGCTGCGGCG GGACCGCCACTGAAATTCGGGGCCGGCCTCCCGGAGACGGGGGGCCGATCCCCAACACCAAGCCCCCGCAATTGG GAGCTCGAGGGTTGAAATGACGCTCGAACAGGCATGCCCGCCAGAATACTGGCGGGCGCAATGTGCGTTCAAAGA TTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCA AGAGATCCGTTGTTGAAAGTTTTAATTTATTTTGTAATGACACTCAGAGAGAATACCACTATAAAGTCAAAGAGT TTTGGTCCCCCGGCGGTCGCCGTGGATCCGGCCGCGGCAGCACAGGACTGCGTGAGGCCGGGGCGTGCCCGCCGA AGCAACGAATAGGTAATGTTCACAAAGGGTTTGGGAGTTGTAAACTCGATAATGATCCCTCCGCTGGTTCACCAA CGGAGACCTTGTTACGACTTTTACTTCCTCTAA 47