VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY THESIS TITLE: ISOLATION AND IDENTIFICATION OF AGARDEGRADING MICROORGANISMS FROM DIVERSE ENVIRONMENTS IN VIETNAM Hanoi, 1/2021 VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY THESIS TITLE: ISOLATION AND IDENTIFICATION OF AGARDEGRADING MICROORGANISMS FROM DIVERSE ENVIRONMENTS IN VIETNAM Student : Vu Thi My Duyen ID : 610710 Department : Biotechnology Supervisor : Vu Nguyen Thanh, Assoc Prof Nguyen Van Giang, Assoc Prof Hanoi, 1/2021 COMMITMENT I hereby declare that: This is my study, which was conducted under the guidance of the supervisors; All data provided are true and accurate; All published data and information have been duly cited Hanoi, January 2021 Student Vu Thi My Duyen i ACKNOWLEDGEMENTS Firstly, I am grateful to express my gratitude to the Food Industries Research Institute, especially to the Center for Industrial Microbiology for granting me support to pursue my thesis as well as to the Department of Biotechnology, Vietnam National University of Agriculture, who provided me opportunities to pursue higher education and to prepare myself to better I also would like to express my gratitude to my supervisor Assoc Prof Nguyen Van Giang for providing me with an opportunity to the final work in the Vietnam National University of Agriculture and giving me all support, which made me complete the project I owe my deep gratitude to Assoc Prof Vu Nguyen Thanh, who has given me all support, guidance, all the necessary information that made me complete the project He allowed me to the necessary research work and use the lab equipment needed at the Center for Industrial Microbiology, Food Industries Research Institute Besides, I would like to extend our sincere esteems to all members in the laboratory for their timely support It also gives my thankfulness to my family, to all of my friends, for sharing my difficulties, and giving me various useful pieces of advice during the process of learning and studying Thank you very much! Hanoi, 2nd February 2021 Student Vu Thi My Duyen ii TABLE OF CONTENTS COMMITMENT i ACKNOWLEDGEMENTS ii TABLE OF CONTENTS iii LIST OF FIGURES v LIST OF TABLES vi ABBREVIATIONS vii ABSTRACT viii I INTRODUCTION II LITERATURE REVIEW 2.1 Introduction of agar 2.1.1 Agar structure 2.1.2 Agar source 2.1.3 Properties of agar 2.2 Agarolytic microorganisms 2.3 Agar-degrading enzymes 2.3.1 Agarase source .6 2.3.2 α-Agarase .7 2.3.3 Applications of agarases 11 2.4 Agar production and agar-degrading enzymes research in Vietnam .13 III MATERIALS AND METHODS 15 3.1 Study laboratory and time 15 3.2 Materials 15 3.2.1 Samples for isolation 15 3.2.2 Chemicals 15 3.2.3 Equipment 16 3.2.4 Media 16 3.3 Research methods (Fu and Kim, 2010) 17 3.3.1 Sample enrichment 17 3.3.2 Method of isolation 17 iii 3.3.3 Purification and maintenance of isolates 17 3.3.4 Observation of colonies and cells 17 3.3.5 Gram staining 17 3.3.6 Protein fingerprinting by SDS-PAGE method 19 3.3.7 DNA extraction and purification 20 3.3.8 rDNA sequencing 21 3.3.9 DNA electrophoresis 21 3.3.11 Agarase assay by Congo red staining 21 IV RESULTS AND DISCUSSION .23 4.1 Isolation results .23 4.2 Selection of agar degrading microorganisms 25 4.3 Observation of colonies, cells .27 4.4 Grouping by protein fingerprinting 29 4.5 Identification of microorganisms by rDNA sequencing 31 V CONCLUSION AND SUGGESTION .35 5.1 Conclusion 35 5.2 Suggestion 35 REFERENCES 36 ANNEX 39 iv LIST OF FIGURES Figure Structure of agarose Figure 2 Diagram of the cleavage site of agarase (Kazłowski, Pan and Ko, 2008) Figure Strategies for agarose hydrolysis and the various physiological activities of agarose-derived sugars 12 Figure Some kinds of samples 23 Figure Isolation origins of 109 agar-degrading strains 24 Figure Enrichment samples were transferred on agar medium at 28 °C after 10 days 24 Figure 4 The morphology of agar-degradable microorganisms Bar, 5µm 28 Figure Electrophoresis images of Protein fingerprinting products of 79 strains .29 Figure Neighbour-joining phylogram depicting the relationships between isolated strains and neighbouring taxa 32 Figure Pie chart comparing frequency appears of microorganisms degrading agar in different samples in Vietnam 33 Figure Congo red staining results of strains cultured on MSYA agar at 28 ° C after days 25 v LIST OF TABLES Table Some agarolytic microorganisms: origins and characteristic of the enzyme Table 2 Characterization of some recombinant β-agarases (Veerakumar and Manian, 2018) .9 Table Experimental content 15 Table Isolation results 23 Table Group of isolated strains by morphology and protein fingerprinting results 30 Table Qualitative agarase activity of representative strains .26 vi ABBREVIATIONS Abbreviations Full name DNA Deoxyribose nucleic acid PCR Polymerase chain reaction rDNA Ribosomal DNA TAE Tris-acetate-EDTA MSYA Minimal salt yeast extract agar CBB Coomassie Brilliant Blue AOS Agarooligosaccharides NAOS Neoagarooligosaccharides vii ABSTRACT In this study, samples were collected from regions in Vietnam to isolate agardegrading microorganisms As we know agar production process still has many shortcomings such as causing environmental pollution, high production costs, etc The research direction of using biotechnology to agar production is currently being interested, in particular the use of enzymes from microorganisms The thesis research for agar-degrading microorganisms, target to produce secondary products of higher value agar, and searched for microorganisms capable of producing enzymes that cut agar into other products such as AOS- agar oligosaccharides and NAOSneoagarooligosaccharides These products are used in pharmaceuticals and cosmetics From 90 samples collected, 109 agar-degrading microorganisms were isolated using morphology and protein fingerprinting method, 44 representative strains randomly of each group one or two strains selected from 61 groups for 16S sequence analysis The results of the sequence analysis identified 19 species including Microbulbifer elongatus, Enterobacter huaxiensi, Alteromonas abrolhosensis, Bacillus flexus, Bacillus tenquilensis, Catenococcus thiocycli, Luteibacter jiangsuensis, Lysinibacillus fusiformis, Microbacterium aquimaris, Nitratireductor indicus, Paracoccus homiensis, Pseudomonas pachastrellae, Salipiger pacificus, Pseudomonas plecoglossicida, Shinella curvata, Sinomicrobium oceani, Shingopyxis granuli, Stappia indica, and Advenellas kashmirensis Four undescribed species of the genera Bacillus, Microbacterium, Microbulbifer, and Shingopyxis have been identified The agarolytic activity of the strains was also investigated Annex List of 109 strains by qualitative activity test using congo red staining Note: (+) positive (-) negative (w) weak No Symbol strain Medium Congo red Group Species (Latin name) ARAS512-2 MSA_Y - Enterobacter huaxiensis ARAS480-2 MSA_Y - Enterobacter huaxiensis ARAS497-1 MSA_Y - Enterobacter huaxiensis ARAS497-2 MSA_Y - Enterobacter huaxiensis ARAS512-1 MSA_Y - Enterobacter huaxiensis ARAS480-4 MSA_Y - Microbulbifer elongatus ARAS480-3 MSA_Y - Microbulbifer elongatus ARAS480-1 MSA_Y - Microbulbifer elongatus ARAS528-1 MSA_Y(NaCl) + Microbulbifer elongatus 10 ARAS536-3 MSA_Y(NaCl) + Microbulbifer elongatus 11 ARAS467-3 MSA_Y(NaCl) + Microbulbifer elongatus 12 ARAS470-3 MSA_Y(NaCl) + Microbulbifer elongatus 13 ARAS517-3 MSA_Y(NaCl) + Microbulbifer elongatus 14 ARAS523-1 MSA_Y(NaCl) W Microbulbifer elongatus 15 ARAS523-2 MSA_Y(NaCl) - Microbulbifer elongatus 16 ARAS539-3 MSA_Y(NaCl) + Stappia indica 17 ARAS539-4 MSA_Y(NaCl) + Stappia indica 18 ARAS539-5 MSA_Y(NaCl) + Stappia indica 19 ARAS539-6 MSA_Y(NaCl) + Stappia indica 20 ARAS529-1 MSA_Y(NaCl) + 21 ARAS529-4 MSA_Y(NaCl) - 22 ARAS530-5 MSA_Y(NaCl) + 23 ARAS525-4 MSA_Y(NaCl) + Microbulbifer elongatus 24 ARAS526-3 MSA_Y(NaCl) + Microbulbifer elongatus 25 ARAS526-7 MSA_Y(NaCl) + Microbulbifer elongatus 26 ARAS460-1 MSA_Y(NaCl) + Microbulbifer elongatus 27 ARAS541-1 MSA_Y(NaCl) + Microbulbifer elongatus 28 ARAS541-2 MSA_Y(NaCl) + Microbulbifer elongatus 29 ARAS533-5 MSA_Y(NaCl) - Paracoccus homiensis 30 ARAS534-2 MSA_Y(NaCl) - Paracoccus homiensis 31 ARAS537-3 MSA_Y(NaCl) + Microbulbifer elongatus 32 ARAS537-4 MSA_Y(NaCl) + Microbulbifer elongatus 33 ARAS526-2 MSA_Y(NaCl) + 10 Microbulbifer elongatus 34 ARAS527-1 MSA_Y(NaCl) + 10 Microbulbifer elongatus 35 ARAS528-2 MSA_Y(NaCl) - 11 Bacillus flexus 36 ARAS541-4 MSA_Y(NaCl) + 11 Bacillus flexus 37 ARAS526-1 MSA_Y(NaCl) + 12 Microbulbifer elongatus 38 ARAS530-1 MSA_Y(NaCl) + 12 Microbulbifer elongatus 39 ARAS461-5 MSA_Y(NaCl) + 13 42 40 ARAS528-3 MSA_Y(NaCl) + 13 41 ARAS537-1 MSA_Y(NaCl) + 14 Bacillus tequilensis 42 ARAS515-1 MSA_Y(NaCl) - 14 Bacillus tequilensis 43 ARAS469-3 MSA_Y(NaCl) - 15 Catenococcus thiocycli 44 ARAS469-4 MSA_Y(NaCl) + 15 Catenococcus thiocycli 45 ARAS542-1 MSA_Y(NaCl) - 16 46 ARAS542-4 MSA_Y(NaCl) - 16 47 ARAS526-5 MSA_Y(NaCl) + 17 48 ARAS526-6 MSA_Y(NaCl) + 17 49 ARAS536-1 MSA_Y(NaCl) W 18 Salipiger pacificus 50 ARAS535-1 MSA_Y(NaCl) W 18 Salipiger pacificus 51 ARAS461-4 MSA_Y(NaCl) - 19 52 ARAS470-1 MSA_Y(NaCl) - 20 Microbulbifer elongatus 53 ARAS470-2 MSA_Y(NaCl) - 20 Microbulbifer elongatus 54 ARAS475-1 MSA_Y - 21 Pseudomonas plecoglossicida 55 ARAS475-2 MSA_Y W 21 Pseudomonas plecoglossicida 56 ARAS483-1 MSA_Y + 22 57 ARAS483-2 MSA_Y + 22 58 ARAS483-3 MSA_Y + 22 59 ARAS519-2 MSA_Y(NaCl) - 23 Pseudomonas pachastrellae 60 ARAS519-3 MSA_Y(NaCl) - 23 Pseudomonas pachastrellae 61 ARAS525-2 MSA_Y(NaCl) + 23 Pseudomonas pachastrellae 62 ARAS533-1 MSA_Y(NaCl) + 24 63 ARAS530-6 MSA_Y(NaCl) + 24 64 ARAS535-2 MSA_Y(NaCl) + 25 Alteromonas abrolhosensis 65 ARAS535-3 MSA_Y(NaCl) + 25 Alteromonas abrolhosensis 66 ARAS538-1 MSA_Y(NaCl) - 26 Microbulbifer elongatus 67 ARAS538-3 MSA_Y(NaCl) + 26 Microbulbifer elongatus 68 ARAS539-2 MSA_Y(NaCl) - 27 Catenococcus thiocycli 69 ARAS541-3 MSA_Y(NaCl) + 27 Catenococcus thiocycli 70 ARAS503-1 MSA_Y + 28 Microbacterim sp.nov 71 ARAS503-2 MSA_Y + 28 Microbacterim sp.nov 72 ARAS517-1 MSA_Y(NaCl) + 29 73 ARAS517-2 MSA_Y(NaCl) + 29 74 ARAS519-1 MSA_Y(NaCl) + 30 Microbulbifer elongatus 75 ARAS458-4 MSA_Y(NaCl) + 30 Microbulbifer elongatus 76 ARAS460-2 MSA_Y(NaCl) + 30 Microbulbifer elongatus 77 ARAS461-1 MSA_Y(NaCl) + 30 Microbulbifer elongatus 78 ARAS502-1 MSA_Y + 31 Microbacterium aquimaris 79 ARAS506-1 MSA_Y + 32 Luteibacter jiangsuensis 80 ARAS469-5 MSA_Y(NaCl) + 33 81 ARAS494-2 MSA_Y - 34 Shinella curvata 82 ARAS526-4 MSA_Y(NaCl) - 35 Microbulbifer elongatus 83 ARAS533-2 MSA_Y(NaCl) - 36 43 84 ARAS536-2 MSA_Y(NaCl) - 37 85 ARAS533-4 MSA_Y(NaCl) + 38 Microbulbifer sp nov 86 ARAS458-1 MSA_Y(NaCl) - 39 Microbulbifer elongatus 87 ARAS458-2 MSA_Y(NaCl) + 40 Microbacterium aquimaris 88 ARAS460-3 MSA_Y(NaCl) + 41 Microbulbifer elongatus 89 ARAS458-3 MSA_Y(NaCl) W 41 Microbulbifer elongatus 90 ARAS461-2 MSA_Y(NaCl) - 42 Bacillus sp nov 91 ARAS479-1 MSA_Y + 43 Sphingopyxis sp nov 92 ARAS482-1 MSA_Y - 44 93 ARAS498-1 MSA_Y - 45 Lysinibacillus fusiformis 94 ARAS520-1 MSA_Y(NaCl) - 46 Advenellas kashmirensis 95 ARAS525-1 MSA_Y(NaCl) - 47 Sinomicrobium oceani 96 ARAS525-6 MSA_Y(NaCl) + 48 Microbulbifer elongatus 97 ARAS530-2 MSA_Y(NaCl) + 49 Microbulbifer elongatus 98 ARAS530-3 MSA_Y(NaCl) + 50 Microbulbifer elongatus 99 ARAS533-3 MSA_Y(NaCl) + 51 Paracoccus homiensis 100 ARAS542-2 MSA_Y(NaCl) - 52 Microbulbifer elongatus 101 ARAS542-3 MSA_Y(NaCl) - 53 102 ARSS315-7 MSA_Y + 54 103 ARSS316-2 MSA_Y - 55 104 ARAS461-3 MSA_Y(NaCl) + 56 105 ARAS464-1 MSA_Y(NaCl) W 57 106 ARAS467-1 MSA_Y(NaCl) W 58 107 ARAS467-2 MSA_Y(NaCl) - 59 108 ARAS491-1 MSA_Y - 60 109 ARAS531-1 MSA_Y(NaCl) + 61 Paracoccus homiensis Microbulbifer elongatus Nitratireductor indicus Annex The rDNA sequences of 44 representative strains >ARAS 458-1 16S 27F-1492R 27F VT3242 TCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGT GGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTG CGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTGG TCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATT GCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGT AGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTGC CAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAGT TAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGGG TAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGGC TCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG ATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTAC GGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCA ACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGTG ACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGT CCTTTGTTGCCAGCGAGTAATGTCGGGAACTC >ARAS 458-2 16S 27F-1492R 27F VT3243 AAGTCGAACGGTGAAGCAGAGCTTGCTCTGTGGATCAGTGGCGAACGGGTGAGTAACACGTGAGCAACCTGCCCT GGACTCTGGGATAAGCGCTGGAAACGGCGTCTAATACTGGATATGAGATGCGATCGCATGGTCGTGTTTGGAAAG 44 ATTTTTCGGTCTGGGATGGGCTCGCGGCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGTCGACGGGT AGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGG AATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCT TTTGGTAGGGAAGAAGCCTTCGGGTGACGGTACCTGCAGAAAAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGG TAATACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGTCTGTCGCGTCTGCTGT GAAATCCCGAGGCTCAACCTCGGGCTTGCAGTGGGTACGGGCAGACTAGAGTGCGGTAGGGGAGATTGGAATTCC TGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGATGGCGAAGGCAGATCTCTGGGCCGTTACTGACG CTGAGGAGCGAAAGGGTGGGGAGCAAACAGGCTTAGATACCCTGGTAGTCCACCCCGTAAACGTTGGGAACTAGT TGTGGGGTCCTTTCCACGGATTCCGTGACGTAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGG CTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGAA CCTTACCAAGGCTTGACATACACCAGAACGGGCCAGAAATGGTCAACTCTTTGGACACTGGTGAACAGGTGGTGC ATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGTTGCC AGCACGTAATGGTGGGAACTCATGG >ARAS 460-3 16S 27F-1492R 27F VT3244 GTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAG TGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTT GCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTG GTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT TGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAG TAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTG CCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAG TTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGG GTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGG CTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAAC GATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTA CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGT GACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTG TCCTTTGTTGCCAGCGAGTAATGTCGGGAACT >ARAS 461-1 16S 27F-1492R 27F VT3245 GAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGTGG GGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCG CTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTGGTC TGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGC ACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTAG GGAGGAAGCCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTGCCA GCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAGTTA AGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGGGTA GTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGGCTC GATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAT GTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTACGG CCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAAC GCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGTGAC AGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCC TTTGTTGCCAGCGAGTAATGTCGGGAACTCA >ARAS 461-2 16S 27F-1492R 27F VT3246 CGAGCGGATTGATGGGAGCTTGCTCCCTGATATCAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTA AGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACTCATTTCCTCGCATGAGGAAATGTTGAAAGGT GGCTTTTAGCTACCACTTACAAATGGACCCGCGGCGCATTACCTAGTTGGTGAGGTAACGGCTCACCAAGGCAAC AATGCGTAGCCGACCTGAAAGGGTGATCGGCCACACTGGGACTGAAACACGGCCCAAACTCCTACGGGAGGCAGC AGTAGGGAATCTTCCGCAATGGACAAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTA AAGCTCTGTTGTTAGGGAAAAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAAAAAGCCAC GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCG CGCAGGTGGTTCCTTAATTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTG AGTGCAGAAGAGGAAAGTGGAATTCCAAGTGTAGCGGTGAAATGCGTAGATATTTGGAGGAACACCAGTGGCGAA GGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT CCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACT CCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTG 45 GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACACCCTAGAGATAGGGCTTTCCCT TCGGGGACAGANTGACAGGGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTGGGTTAAGTCC >ARAS 467-1 16S 27F-1492R 27F VT3247 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGCCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCC TTGTCCTTTGTTGCCAGCGAGTAATGTCGGG >ARAS 469-3 16S 27F-1492R 27F VT3248 GACGTTAACGGCGTCGAGCGGCGGACGGGTGAGTAATGCCTAGGAAATTGCCCTGATGTGGGGGATAACCATTGG AAACGATGGCTAATACCGCATGATGCCTACGGGCCAAAGAGGGGGACCTTCGGGCCTCTCGCGTCAGGATATGCC TAGGTGGGATTAGCTAGTTGGTGAGGTAAGGGCTCACCAAGGCGACGATCCCTAGCTGGTCTGAGAGGATGATCA GCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG CCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTCGTGAGGAAGGTTTAT GCGTTAATAGCGTATAGATTTGACGTTAGCGACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT ACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGCAGGTGGTTTGTTAAGTCAGATGTGAAA GCCCGGGGCTCAACCTCGGAATAGCATTTGAAACTGGCAGACTAGAGTACTGTAGAGGGGGGTAGAATTTCAGGT GTAGCGGTGAAATGCGTAGAGATCTGAAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGATACTGACACTCA GATGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCTACTTGGAGGT TGTGGCCTTGAGCCGTGGCTTTCGGAGCTAACGCGTTAAGTAGACCGCCTGGGGAGTACGGTCGCAAGATTAAAA CTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTAC CTACTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATGGC TGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTGTTTGCCAGCGA GTAATGTCGG >ARAS 470-1 16S 27F-1492R 27F VT3250 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCC TTGTCCTTTGTTGCCAGCGAGTAATGTCGGGA >ARAS 475-1 16S 27F-1492R 27F VT3251 GCAAGTCGAGCGGATGACGGGAGCTTGCTCCTTGATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCT GGTAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGACCTTCGG GCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATCCGT AACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGG AATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACT TTAAGTTGGGAGGAAGGGCAGTAAGCTAATACCTTGCTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACT CTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTG 46 GTTCGTTAAGTTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCGAGCTAGAGTACGGT AGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCA CCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCG TAAACGATGTCAACTAGCCGTTGGAATCCTTGAGATTTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGG GAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTC GAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAAC TCTGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAAC CCTTGTCCTTAGTTACCAGCACGTTATGGTGGGCACTCTAA >ARAS 479-1 16S 27F-1492R 27F VT3252 AAGTCGAACGAAGTCTTCGGACTTAGTGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTTGGGTACGGAATA ACTCGGAGAAATTCGTGCTAATACCGTATAATGTCTTCGGACCAAAGATTTATCGCCCAAGGATGAGCCCGCGTA GGATTAGCTAGTTGGTGAGGTAAAAGCTCACCAAGGCGACGATCCTTAGCTGGTCTGAGAGGATGATCAGCCACA CTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT CCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTTACCAGGGATGATAATGACAGTACCT GGAGAATAAGCTCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTACT GGGCGTAAAGCGCGCGTAGGCGGTTTTCCAAGTCAGGGGTGAAAGCCCGGGGCTCAACCCCGGAATAGCCCTTGA AACTGGAAGACTAGAATCTTGGAGAGGTCAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGA ACACCAGTGGCGAAGGCGACTGACTGGACAAGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTA GATACCCTGGTAGTCCACGCCGTAAACGATGATAACTAGCTGTCCGGGTTCACAGAACTTGGGTGGCGCAGCTAA CGCATTAAGTTATCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCTGCACAAGC GGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCGTTTGACATCCTGATCGCGGATAGTGG AGACACTTTCCTTCAGTTCGGCTGGATCAGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTT GGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCCTAGTTGCCAGCATTTAGTTGGG >ARAS 491-1 16S 27F-1492R 27F VT3255 AGTCGAGCGCCCCGCAAGGGGAGCGGCAGACGGGTGAGTAACGCGTGGGAATCTACCTAGCTCTACGGAATAACT CAGGGAAACTTGAACTAATACCGTATACGCCCTTCGGGGGAAAGATTTATCGGAGTTGGATGAGCCCGCGTCTGA TTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCAGTAGCTGGTCTGAGAGGATGATCAGCCACACTG GGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCA GCCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTCACCGGTGAAGATAATGACGGTAACCGGA GAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGG CGTAAAGCGCACGTAGGCGGACCATTAAGTCAGGGGTGAAATCCCGGGGCTCAACCCCGGAACTGCCTTTGATAC TGGTGGTCTCGAGTTCGAGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAGGAACA CCAGTGGCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGAT ACCCTGGTAGTCCACGCCGTAAACGATGGAAGCTAGCCGTTGGCAGGTTTACCTGTCGGTGGCGCAGCTAACGCA TTAAGCTTCCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTG GAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCCTTGACATCTGGGTCGCGGAAACCGGAGAC GGTTTCCTTCAGTTAGGCTGGACCCAAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGT TAAGTCCCGCAACGAGCGCAACCCTCGCCCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGGGACTGCCGGTG ATAAGCCGAGAGGAAGGTGGGGATGACGTCA >ARAS 494-2 16S 27F-1492R 27F VT3256 TGCAAGTCGAACGCCCCGCAAGGGGAGTGGCAGACGGGTGAGTAACGCGTGGGAACGTACCCTTTACTACGGAAT AACTCAGGGAAACTTGTGCTAATACCGTATGTGCCCTTCGGGGGAAAGATTTATCGGTAAAGGATCGGCCCGCGT TGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAGGATGATCAGCCAC ATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGA TCCAGCCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTCACCGGTGAAGATAATGACGGTAAC CGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC TGGGCGTAAAGCGCACGTAGGCGGGTATTTAAGTCAGGGGTGAAATCCCAGAGCTCAACTCTGGAACTGCCTTTG ATACTGGGTACCTAGAGTATGGAAGAGGTAAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAGG AACACCAGTGGCGAAGGCGGCTTACTGGTCCATTACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATT AGATACCCTGGTAGTCCACGCCGTAAACGATGAATGTTAGCCGTCGGCATGCATGCATGTCGGTGGCGCAGCTAA CGCATTAAACATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC GGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCCTTGACATGTCGGTCGCGGATTACAG AGATGTTTTCCTTCAGTTAGGCTGGACCGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTT GGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAG >ARAS 497-1 16S 27F-1492R 27F VT3257 GCGAGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAA TACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAG 47 CTAGTAGGTGGGGTAATGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAAC TGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA TGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGTGATGAGGTTAATAACCT CGTCAATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA GCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAA CCTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAAT GCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGT GGAGAGCAAACAGGATTAGATACTCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGG CGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGAGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTG ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTACTCTTGACAT CCAGAGAACTTAGCAGAGATGCTTTGGTGCCTTCGGGAACTCTG >ARAS 498-1 16S 27F-1492R 27F VT3258 GTCGAGCGAACAGAGAAGGAGCTTGCTCCTTCGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTACC TTATAGTTTGGGATAACTCCGGGAAACCGGGGCTAATACCGAATAATCTGTTTCACCTCATGGTGAAATACTGAA AGACGGTTTCGGCTGTCGCTATAGGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGC GACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGC AGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGATTTCGGTTC GTAAAACTCTGTTGTAAGGGAAGAACAAGTACAGTAGTAACTGGCTGTACCTTGACGGTACCTTATTAGAAAGCC ACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCG CGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACT TGAGTGCAGAAGAGGATAGTGGAATTCCAAGTGTAGCGGTGAAATGCGTAGAGATTTGGAGGAACACCAGTGGCG AAGGCGACTATCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTA GTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCA CTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATG TGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCGTTGACCACTGTAGAGATATAGTTTC CCCTTCGGGGGCAACGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCG >ARAS 502-1 16S 27F-1492R 27F VT3259 CGAACGGTGAAGCAGAGCTTGCTCTGTGGATCAGTGGCGAACGGGTGAGTAACACGTGAGCAACCTGCCCTGGAC TCTGGGATAAGCGCTGGAAACGGCGTCTAATACTGGATATGAGATGCGATCGCATGGTCGTGTTTGGAAAGATTT TTCGGTCTGGGATGGGCTCGCGGCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGTCGACGGGTAGCC GGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTTG GTAGGGAAGAAGCCTTCGGGTGACGGTACCTGCAGAAAAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAAT ACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGTCTGTCGCGTCTGCTGTGAAA TCCCGAGGCTCAACCTCGGGCTTGCAGTGGGTACGGGCAGACTAGAGTGCGGTAGGGGAGATTGGAATTCCTGGT GTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGATGGCGAAGGCAGATCTCTGGGCCGTTACTGACGCTGA GGAGCGAAAGGGTGGGGAGCAAACAGGCTTAGATACCCTGGTAGTCCACCCCGTAAACGTTGGGAACTAGTTGTG GGGTCCTTTCCACGGATTCCGTGACGTAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAA AACTCAAAGGAATTGACGGGGACCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTT ACCAAGGCTTGACATACACCAGAACGGGCCAGAAATGGTCAACTCTTTGGACACTGGTGAACAGGTGGTGCATGG TTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGTTGCCAGCA CGTAATGGTGGGAACTCATGGG >ARAS 503-1 16S 27F-1492R 27F VT3260 AACGGTGAAGCCAAGCTTGCTTGGTGGATCAGTGGCGAACGGGTGAGTAACACGTGAGCAACCTGCCCTGGACTC TGGGATAAGCGCTGGAAACGGCGTCTAATACTGGATATGAGCCTTCATCGCATGGTGGGGGTTGGAAAGATTTTT TGGTCTGGGATGGGCTCGCGGCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGTCGACGGGTAGCCGG CCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATT GCACAATGGGCGGAAGCCTGATGCAGCAACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTTAGC AAGGAAGAAGCTTTTGTGACGGTACTTGCAGAAAAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGT AGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGTTTGTCGCGTCTGCTGTGAAATCCC GAGGCTCAACCTCGGGCCTGCAGTGGGTACGGGCAGACTAGAGTGCGGTAGGGGAGATTGGAATTCCTGGTGTAG CGGTGGAATGCGCAGATATCAGGAGGAACACCGATGGCGAAGGCAGATCTCTGGGCCGTAACTGACGCTGAGGAG CGAAAGGGTGGGGAGCAAACAGGCTTAGATACCCTGGTAGTCCACCCCGTAAACGTTGGGAACTAGTTGTGGGGT CCTTTCCACGGATTCCGTGACGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACT CAAAGGAATTGACGGGGACCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCA AGGCTTGACATACACGAGAACATCCCAGAAATGGGGGACTCTTTGGACACTCGTGAACAGGTGGTGCATGGTTGT CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGTTGCCAGCACGTA ATGGTGGGAACTCATGGGATACTGCCGGGGTCAACTCGGAGGAAGGGGGGATGACGTCAAATCATCATGCC 48 >ARAS 506-1 16S 27F-1492R 27F VT3261 GTCGAACGGCAGCACAGCAGAGCTTGCTCTGTGGGTGGCGAGTGGCGGACGGGTGAGTAATGCATCGGGACCTAC CCAGACGTGGGGGATAACGTAGGGAAACTTACGCTAATACCGCATACGTCCTACGGGAGAAAGCGGGGGACCTTT TAGGCCTCGCGCGGTTGGATGGACCGATGTGCGATTAGCTTGTTGGTGAGGTAACGGCTCACCAAGGCGACGATC GCTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTG GGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGC ACTTTTATCAGGAGCGAAATCTGCAGGGCTAATACCCATGCAGTCTGACGGTACCTGAGGAATAAGCACCGGCTA ACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTAG GCGGTTGGTTAAGTCTGCTGTGAAAGCCCCGGGCTCAACCTGGGAATGGCAGTGGATACTGGCTAGCTAGAGTGT GATAGAGGATGGTGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACATCAGTGGCGAAGGCGG CCATCTGGATCAACACTGACGCTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG CCCTACACGATGCGAACTGGATGTTGGTCTCAACTCGGAGATCAGTGTCGAAGCTAACGCGTTAAGTTCGCCGCC TGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTA ATTCGATGCAACGCGAAGAACCTTACCTGGCCTTGACATGTCCGGAATCCTGCAGAGATGCGGGAGTGCCTTCGG GAATCGGA >ARAS 527-1 16S 27F-1492R 27F VT3262 AAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGT AGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACC TTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCAACGATCCGTAGC TGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT ATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTC AGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCG TGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTT AGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGA GGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCT GGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAA ACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAG TACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAA GCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCT GTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCT TGTCCTTTGTTGCCAGCGAGTAATGTCGG >ARAS 517-3 16S 27F-1492R 27F VT3263 AGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTA GTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCT TGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCT GGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCA GTAGGGAGGAAGCCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGT GCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTA GTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAG GGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTG GCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAA CGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGT ACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAG CAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTG TGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTT GTCCTTTGTTGCCAGCGAGTAATGTCGGGAA >ARAS 519-1 16S 27F-1492R 27F VT3264 TCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGT GGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTG CGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTGG TCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATT GCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGT AGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTGC CAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAGT TAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGGG TAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGGC TCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACG 49 ATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTAC GGCCGCAAGGTTAAGACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCA ACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGTG ACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGA >ARAS 519-2 16S 27F-1492R 27F VT3265 GTCGAGCGGTAGAGAAGGAGCTTGCTTCTTCTTGAGAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGG TAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAAAGTGGGGGATCTTCGGAC CTCACGCTATCAGATGAGCCTAGGTCGGATTAGCTTGTAGGTGAGGTAATGGCTCACCTAGGCAACGATCCGTAA CTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTT AAGTTGGGAGGAAGGGTTGTAGCTTAATACGCTGCAACTTTGACGTTACCAACAGAATAAGCACCGGCTAACTCT GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGC TTGATAAGATGGGTGTGAAATCCCCGGGCTTAACCTGGGAACTGCATCCATAACTGTCTGGCTAGAGTGCAGTAG AGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACC TGGACTGACACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCAACTAGCCGTTGGAATCCTTGAGATTTTAGTGGCGCAGCTAACGCACTAAGTTGACCGCCTGGGGA GTACGGTCGCAAGATTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCTGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTC >ARAS 520-1 16S 27F-1492R 27F VT3266 GCAAGTCGAACGGCAGCGGGAAAGTAGCTTGCTACTTTTGCCGGCGAGTGGCGAACGGGTGAGTAATGTATCGGA ACGTGCCCAGTAGCGGGGGATAACTACGCGAAAGCGTGGCTAATACCGCATACGCCCTACGGGGGAAAGGGGGGG ATCTTAGGACCTCTCACTATTGGAGCGGCCGATATCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGA CGATCCGTAGCTGGTTTGAGAGGACGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG CAGTGGGGAATTTTGGACAATGGGGGAAACCCTGATCCAGCCATCCCGCGTGTGCGATGAAGGCCTTCGGGTTGT AAAGCACTTTTGTCAGGGAAGAAAAGGTTTCGGATAATACCTGGAACTGATGACGGTACCTGAAGAATAAGCACC GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTG CGCAGGCGGTTCGGAAAGAAAGATGTGAAATCCCAGGGCTCAACCTTGGAACTGCATTTTTAACTCCCGAACTAG AGTATGTCAGAGGGGGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGATATGTGGAGGAACACCGATGGCGAA GGCAGCCCCCTGGGATAATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT CCACGCCCTAAACGATGTCAACTAGCTGTTGGGCCCTTCGGGGCTTAGTAGCGCAGCTAACGCGTGAAGTTGACC GCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGA TTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGTCTGGAATCCTGAAGAGATTTAGGAGTGCTCG CAAGAGAACCGGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTG >ARAS 525-1 16S 27F-1492R 27F VT3267 GCAAGTCGAGGGGTATAAATAGCTTGCTATTTAGAGACCGGCGCACGGGTGCGTAACGCGTATGCAATCTACCTT ATGCAGAGGGATAGCCCGAAGAAATTTGGATTAATACCTCATAGTATATAGAATAGGCCTCTATTTTATATTAAA GATTTATCGGCATAAGATGAGCATGCGTCCCATTAGCTAGTTGGTATGGTAACGGCATACCAAGGCAACGATGGG TAGGGGTCCTGAGAGGGAGATCCCCCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCAGTGAG GAATATTGGACAATGGTCGCAAGACTGATCCAGCCATGCCGCGTGCAGGAAGACTGCCCTATGGGTTGTAAACTG CTTTTGTATGAGAAGAATAAGAGGTACGTGTACCTTGATGACGGTATCATACGAATAAGCATCGGCTAACTCCGT GCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTAGGCGGGCT ATTAAGTCAGGGGTGAAAGTTTGCCGCTCAACGGTAAAATTGCCTTTGATACTGATAGTCTTGAATGTGTGTGAA GTGATTAGAATATGTAGTGTAGCGGTGAAATGCATAGATATTACATAGAATACCGATTGCGAAGGCAGGTCACTA ACACATTATTGACGCTGATGGACGAAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAA CGATGGATACTAGCTGTTGGGACTTCGGTTTCAGTGGCTAAGCGAAAGTGATAAGTATCCCACCTGGGGAGTACG TTCGCAAGAATGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGATA CGCGAGGAACCTTACCAAGGCTTAAATGTAGTGTGACAGGACTGGAAACAGTTTTTTCTTCGGACACATTACAAG GTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCAGGTTAAGTCCTATAACGAGCGCAACCCCTGT >ARAS 525-6 16S 27F-1492R 27F VT3268 GCAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCA GTAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGA CCTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTA GCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGA ATATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTT TCAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTC CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGG 50 TTAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGA GAGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGC CTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGT AAACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGG AGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCG AAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACT CTGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACG >ARAS 526-3 16S 27F-1492R 27F VT3270 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGCCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCC TTGTCCTTTGTTGCCAGCGAGTAATGTCGGGAA >ARAS 526-4 16S 27F-1492R 27F VT3271 GTCGAGCGCGAACGGTCCTTCAGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTA GTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCT TGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCT GGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCA GTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGT GCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTA GTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAG GGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTG GCTCGATACTGACGCTGAGGTGCGAAAGCGTGCGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAA CGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGT ACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAG CAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTG TGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTT GTCCTTTGTTGCCAGCGAGTAATGTCGGGAA >ARAS 528-1 16S 27F-1492R 27F VT3273 GGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATA CGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGT GGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACG GCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTG TGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTG ACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATC GGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAAC TGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATA TAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGGCTCGATACTGACGCTGACGTGCGAAAGCGTGTGGAGCAA ACAGGATTAGATACTCTGGTAGTCCACGCCGTACACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTGTGTGA CGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAGACTCAAATGAATTGACGGAGGCC CGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAAC TTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAG ATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCTTTGTTGCCAGCGAGTAATGTCGGGAACTCAAAGGAG ACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAGTCATCATG >ARAS 528-2 16S 27F-1492R 27F 51 VT3274 TGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAAC CTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAACATTTTCTCTTGCATAAGAGAAAA TTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACC AAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG GAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTC GGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGA AAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTA AAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG GAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAG TGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCC TGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATT AAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGA GCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAG CGTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAG TCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATT >ARAS 530-1 16S 27F-1492R 27F VT3275 GCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACC GCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTG TTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAG ACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCCATGCC GCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGG GATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGT TAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTG GGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGT AGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGG AGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTT TGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGG GGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAG AGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTC GTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTTGTTGCCAGCG >ARAS 530-2 16S 27F-1492R 27F VT3276 TCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGT GGGGGATAGCCCGGGGTAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTT GCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAGCTG GTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT TGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAG TAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCCGTG CCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAG TTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAGAGG GTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGG CTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTACAC GATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGAGTA CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGT GACAGGTGCTGCATGGCTGACGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTG TCCTTTGTTGCCAGCGAGTAATGTCGGGAAC >ARAS 530-3 16S 27F-1492R 27F VT3277 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA 52 AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGT >ARAS 533-3 16S 27F-1492R 27F VT3280 GTCGAGCGAGATCTTCGGATCTAGCGGCGGACGGGTGAGTAACGCGTGGGAACGTGCCCTTTGCTACGGAATAGT CCCGGGAAACTGGGTTTAATACCGTATGTGCCCTTCGGGGGAAAGATTTATCGGCAAAGGATCGGCCCGCGTTGG ATTAGGTAGTTGGTGGGGTAATGGCCTACCAAGCCGACGATCCATAGCTGGTTTGAGAGGATGATCAGCCACACT GGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGATCT AGCCATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTCAGCTGGGAAGATAATGACGGTACCAGC AGAAGAAGCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTACTGG GCGTAAAGCGCACGTAGGCGGACTGGAAAGTTGGGGGTGAAATCCCGGGGCTCAACCTCGGAACTGCCTCCAAAA CTATCAGTCTGGAGTTCGAGAGAGGTGAGTGGAATACCGAGTGTAGAGGTGAAATTCGTAGATATTCGGTGGAAC ACCAGTGGCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGA TACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGTCGTCGGGTTGCATGCAATTCGGTGACACACCTAACGG ATTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGT GGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAACCCTTGACATCCCAGGACCGATCCAGAGATG GATCTTTCACTTCGGTGACCTGGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAA GTCCGGCAACGAGCGCAACCCACGTCCCTAGTTGCCAGCATTCAGTTGGGCACTCTATGGAAACTGCCGATGATA AG >ARAS 533-4 16S 27F-1492R 27F VT3281 CGAGCGCGAACGGTCCTTCGTGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAGTAGT GGGGGATAGCCCGGGGTAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGACCTT GCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTACCTG GTCTGAGAGGATGATCAGCCACACTGGGACTGAAACACGGCCCAGACTCCTACGGGAGGCAACGGTGGGGAATAT TGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAAGCCTTCGGGTTGTAAAGCACTTTCAT TAGGGAGGAAAGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAACACCGGCTAACTCCGTG CCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGTTAG TTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGACTGGCTAGAGTACAAGAGAGG ATAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCCTGG CTCGATACTGACGCTGAGGTGCGAAAGCATGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTACAC GATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACTCAATAAGTAGACCGCCTGGCGAGTA CAGCCGCAAGGTTAGAACTCAAATGAATTGACGGGGGCCCGCACAACCGATGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAAACTTACCTAGTCTTGACTTCCATAGAACTTACCATACATGGATTGATGCCTTCCGGAACTCAGT GACAGG >ARAS 534-2 16S 27F-1492R 27F VT3282 TGCAAGTCGAGCGAGATCTTCGGATCTAGCGGCGGACGGGTGAGTAACGCGTGGGAACGTGCCCTTTGCTACGGA ATAGTCCCGGGAAACTGGGTTTAATACCGTATGTGCCCTTCGGGGGAAAGATTTATCGGCAAAGGATCGGCCCGC GTTGGATTAGGTAGTTGGTGGGGTAATGGCCTACCAAGCCGACGATCCATAGCTGGTTTGAGAGGATGATCAGCC ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCT GATCTAGCCATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTCAGCTGGGAAGATAATGACGGTA CCAGCAGAAGAAGCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATT ACTGGGCGTAAAGCGCACGTAGGCGGACTGGAAAGTTGGGGGTGAAATCCCGGGGCTCAACCTCGGAACTGCCTC CAAAACTATCAGTCTGGAGTTCGAGAGAGGTGAGTGGAATACCGAGTGTAGAGGTGAAATTCGTAGATATTCGGT GGAACACCAGTGGCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGA TTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGTCGTCGGGTTGCATGCAATTCGGTGACACACCT AACGGATTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAA GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAACCCTTGACATCCCAGGACCGATCCAG AGATGGATCTTTCACTTCGGTGACCTGGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTCG GTTAAGTCCGGCAACGAGCGCAACCCACGTCCCTAGTTGCCAGCATTCAGTTGGGCACTCTATGGAAACTGCCGA TGATAAGTCG >ARAS 535-2 16S 27F-1492R 27F VT3283 TAGCTTGCTAGAAGATGACGAGTGGCGGACGGGTGAGTAATGCTTGGGAACTTGCCTTTGCGAGGGGGATAACAG TTGGAAACGACTGCTAATACCGCATAATGTCTTCGGACCAAACGGGGCTTCGGCTCCGGCGCAAAGAGAGGCCCA AGTGAGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCTCTAGCTGTTCTGAGAGGAAGATCAGC CACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC TGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTTGTGAGGAAAAGTTAGTA GTTAATACCTGCTAGCCGTGACGTTAACAACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC GGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGCTAGATGTGAAAGC 53 CCCGGGCTCAACCTGGGATGGTCATTTAGAACTGGCAGACTAGAGTCTTGGAGAGGGGAGTGGAATTCCAGGTGT AGCGGTGAAATGCGTAGATATCTGGAGGAACATCAGTGGCGAAGGCGACTCCCTGGCCAAAGACTGACGCTCATG TGCGAAAGTGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGCTGTCTACTAGCTGTGTG TGCCTTTAAGGCGTGCGTAGCGAAGCTAACGCGCTAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT CAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCT ACACTTGACATGCTGAGAACTTACTAGAGATAGATTCGTGCCTTCGGGAACTCTGACACAGGTGCTGCATGGCTG TCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCCAGCCTTA AGTT >ARAS 536-1 16S 27F-1492R 27F VT3284 GCGAGACCTTCGGGTCTAGCGGCGGACGGGTGAGTAACGCGTGGGAACGTGCCCTTCTCTACGGAATAGTCCCGG GAAACTGGGTTTAATACCGTATACGCCCTTCGGGGGAAAGATTTATCGGAGAAGGATCGGCCCGCGTTAGATTAG GTAGTTGGTGGGGTAATGGCCTACCAAGCCTACGATCTATAGCTGGTTTGAGAGGATGATCAGCCACACTGGGAC TGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGATCTAGCCA TGCCGCGTGAGTGATGAAGGCCTTAGGGTCGTAAAGCTCTTTCGCTGGGGAAGATAATGACTGTACCCAGTAAAG AAACCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTA AAGCGCGCGTAGGCGGATTGGAAAGTTGGGGGTGAAATCCCGGGGCTCAACCTCGGAACTGCCTCCAAAACTCCC AGTCTTGAGTTCGAGAGAGGTGAGTGGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG TGGCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGTGTGGGGAGCAAACAGGATTAGATACCC TGGTAGTCCACACCGTAAACGATGAATGCCAGTCGTCGGCAAGCATGCTTGTCGGTGACACACCTAACGGATTAA GCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGC ATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAACCCTTGACATCCTCGGACCGCCAGAGAGATCTGGTT TTCACTTCGGTGACCGAGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCG GCAACGAGCGCAACCCACATCTTCAGTTGCCAGCAGTTCGGCTGGG >ARAS 537-1 16S 27F-1492R 27F VT3285 GTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTG TAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAG GTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAA CGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG CAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGT AAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCA CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGC TCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTT GAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGA AGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAG TCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCAC TCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCC CTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGG >ARAS 537-4 16S 27F-1492R 27F VT3286 GTCGAGCGCGAACGGTCCTATCGTGTGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TACTGGGGGATAGCCCGGGGTAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAACAGGGGATCTTCTGA CCTTGCGCTATTGGATGAGCCTATGTCAGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTA CCTGGTCTGATAGGATGATCAGCCACACTGGGACTGAAACACGGCCCAGACTCCTACCGGAGGCAACGGTGGGGA ATATTGCACAATGGGGGAAACCCTGATGCAACCATGCCGCGTGTGTGAAGAAAGCCTTCGGGTTGTAAAGCACTT TCATTACGGAGGAAAGCCCTAAAGTTAATACCTTTACGGATTGAAATTACCTACATAACAAACACCGGCTAACTC CGTGCCCAGCACCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCACGCGTAGGCG GTTAGTTAAGCTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGACTGGCTAGAGTACAAG AGAGGATAGTGGAATTTCCTGTGTAGCGGTGAGATGCGTAGATATAAGAAGGAACATCAGTGGCGAAGGCGACTG CCTGGCTCGATACTGACTCTGAGGTGCGAAGAGCGTGGCGTAGCAAACCAGGATTAGATTACCCTGCCTAGTCCA CGCCGTAACACAATGTCATACTAGACCATACGGTTCCCTTGACGGACTTTGTTGACGCAGCTAACCTCATAAGAT AGACCGCCCTGGCGGAGTACAGTCCGCAACGGTTAAGATCTCAAATGAAATTGAACTGGAGGCGCGCCACAACCC GAT >ARAS 538-3 16S 27F-1492R 27F VT3287 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG 54 CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGCCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA AACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCACCCT TGTCCTTTGTTGCCAGCGAGTAATGTCGGGAA >ARAS 539-3 16S 27F-1492R 27F VT3288 CGAACGGTCTCTTCGGAGGCAGTGGCAGACGGGTGAGTAACGCGTGGGAACATACCTTTCGGTACGGAACAACAG TTGGAAACGACTGCTAATACCGTATACGCCCTGAGGGGGAAAGATTTATCGCCGAGAGATTGGCCCGCGTTGGAT TAGCTAGTTGGTGGGGTAATGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAGGATGATCAGCCACACTGG GACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAG CCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTCGCCGGTGAAGATAATGACGGTAACCGGTA AAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGC GTAAAGCGCACGTAGGCTGACTTTTAAGTCAGGGGTGAAATCCCGGGGCTCAACCTCGGAACTGCCTTTGATACT GGAAGTCTTGAGTCCGAGAGAGGTGAGTGGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACAC CAGTGGCGAAGGCGGCTCACTGGCTCGGTACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA CCCTGGTAGTCCACGCCGTAAACGATGGAAGCTAGCTGTCAGGTAGCATGCTATTTGGTGGCGCAGCTAACGCAT TAAGCTTCCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGG AGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCCTTGACATGTCCGGCACACACCAGAGATGGT GTTTTCCCTTCGGGGACCGGAGCACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGT CCCGCAACGAGCGCAACCCTCGCCCTTAGTTGCCAGCATTCAGTTG >ARAS 541-1 16S 27F-1492R 27F VT3289 CAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCAG TAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGAC CTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTAG CTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA TATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTT CAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTCC GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGT TAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGAG AGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGCC TGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCACACAGGATTAGATACCCTGGTAGTCCACGCCGTA CACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTC TGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCC TTGTCCTTTGTTGCCAGCGAGT >ARAS 541-3 16S 27F-1492R 27F VT3290 GTCGAGCGGAACGAGTTAACTGAACCTTCGGGGAACGTTAACGGCGTCGAGCGGCGGACGGGTGAGTAATGCCTA GGAAATTGCCCTGATGTGGGGGATAACCATTGGAAACGATGGCTAATACCGCATGATGCCTACGGGCCAAAGAGG GGGACCTTCGGGCCTCTCGCGTCAGGATATGCCTAGGTGGGATTAGCTAGTTGGTGAGGTAAGGGCTCACCAAGG CGACGATCCCTAGCTGGTCTGAGAGGATGATCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGG CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGT TGTAAAGCACTTTCAGTCGTGAGGAAGGTTTATGCGTTAATAGCGTATAGATTTGACGTTAGCGACAGAAGAAGC ACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGC GCATGCAGGTGGTTTGTTAAGTCAGATGTGAAAGCCCGGGGCTCAACCTCGGAATAGCATTTGAAACTGGCAGAC TAGAGTACTGTAGAGGGGGGTAGAATTTCAGGTGTAGCGGTGAAATGCGTAGAGATCTGAAGGAATACCGGTGGC GAAGGCGGCCCCCTGGACAGATACTGACACTCAGATGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGT AGTCCACGCCGTAAACGATGTCTACTTGGAGGTTGTGGCCTTGAGCCGTGGCTTTCGGAGCTAACGCGTTAAGTA GACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATG TGGTTTAATTCGATGCAACGCGAAGAACCTTACCTACTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTG CCTTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGT 55 >ARAS 542-2 16S 27F-1492R 27F VT3291 GCAAGTCGAGCGCGAACGGTCCTTCGGGACTTATTAGAGCGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCA GTAGTGGGGGATAGCCCGGGGAAACCCGGATTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGATCTTCGGA CCTTGCGCTATTGGATGAGCCTATGTCGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCGTA GCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGA ATATTGCACAATGGGGGAAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTT TCAGTAGGGAGGAAGGCCCTAAAGTTAATACCTTTAGGGATTGACGTTACCTACAGAAGAAGCACCGGCTAACTC CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGG TTAGTTAAGCTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTCAGAACTGGCTGGCTAGAGTACGAGA GAGGGTAGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACATCAGTGGCGAAGGCGACTGC CTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGT AAACGATGTCTACTAGTCGTAGGGTTCCTTGAGGACTTTGTGACGCAGCTAACGCAATAAGTAGACCGCCTGGGG AGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCG AAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACT CTGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACC C >ARSS 315-7 16S 27F-1492R 27F VT3292 AGATCTTCGGATCTAGCGGCGTGACTGGTGAGTAACGCGTGGGANCGTGCCCTTTGCTACGGAATAGTCCCGGGA AACTGGGTTTAATACCGTATGTGCCCTTCGGGGGAAAGATTTATCGGCAAAGGATCGGCCCGCGTTGGATTAGGT AGTTGGTGGGGTAATGGCCTACCAAGCCGACGATCCATAGCTGGTTTGAGAGGATGATCAGCCACACTGGGACTG AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGATCTAGCCATG CCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTCAGCTGGGAAGATAATGACGGTACCAGCAGAAGAA GCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAA GCGCACGTAGGCGGACTGGAAAGTTGGGGGTGAAATCCCGGGGCTCAACCTCGGAACTGCCTCCAAAACTATCAG TCTGGAGTTCTAGAGAGGTGAGTGGAATACCGAGTGTAGAGGTGAAATTCGTAGATATTCGGTGGAACACCAGTG GCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTG GTAGTCCACGCCGTAAACGATGAATGCCAGTCGTCGGGTTGCATGCAATTCGGTGACACACCTAACGGATTAAGC ATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGAGCCCGCACAAGCGGTGGAGCAT GTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAACCCTTGACATCTCAGGACCGATCCAGAGATGGATCTTT CACTTCGGTGACCTGGAAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAAATGTTCGGTTAAGT 56