Nghiên cứu tăng hàm lượng khoáng có trong tảo xoắn spirulina

HỌC VIỆN NÔNG NGHIỆP VIỆT NAM KHOA CÔNG NGHỆ SINH HỌC - - KHÓA LUẬN TỐT NGHIỆP ĐỀ TÀI: NGHIÊN CỨU TĂNG HÀM LƢỢNG KHỐNG CĨ TRONG TẢO XOẮN SPIRULINA Ngƣời thực : Phạm Văn Hào Mã sinh viên : 637316 Giảng viên hƣớng dẫn : PGS.TS.Nguyễn Đức Bách Địa điểm thực : Viện nghiên cứu Vi tảo Dƣợc Mỹ phẩm Hà Nội, 2023 LỜI CAM ĐOAN Tôi xin cam đoan kết quả, hình ảnh, số liệu nghiên cứu đƣợc sử dụng luận văn trung thực, chƣa đƣợc sử dụng báo cáo Tất thơng tin khóa luận đƣợc trích dẫn Tơi xin chịu trách nhiệm lời cam đoan trƣớc Học viện Hội đồng Hà Nội, ngày… tháng… năm 2022 Sinh viên Phạm Văn Hào LỜI CẢM ƠN Tôi xin gửi lời cảm ơn chân thành đến Ban chủ nhiệm khoa Công nghệ sinh học tồn thể Thầy, Cơ truyền đạt cho kiến thức chuyên ngành, kỹ làm việc học quý báu suốt thời gian học tập, rèn luyện Học viện Nơng nghiệp Việt Nam Formatted: Font: Bold Để hồn thành khóa luận, ngồi nỗ lực cố gắng thân, nhận đƣợc giúp đỡ, động viên tích cực từ PGS.TS.Nguyễn Đức Bách, tập thể cán Viện nghiên cứu Vi tảo Dƣợc Mỹ phẩm Trong thời gian thực tập Viện Nghiên cứu Vi tảo Dƣợc mỹ phẩm, Học viện Nông nghiệp Việt Nam, nhận đƣợc quan tâm, bảo tận tình Thầy, Cơ, với cố gắng, nỗ lực thân học kinh nghiệm tơi hồn thành khóa luận tốt nghiệp Tơi xin gửi lời cảm ơn chân thành đến Ban chủ nhiệm khoa Cơng nghệ sinh học tồn thể Thầy, Cô truyền đạt cho kiến thức chuyên ngành, kỹ làm việc học quý báu suốt thời gian học tập, rèn luyện Học viện Nông nghiệp Việt Nam Tôi xin bày tỏ lòng biết ơn sâu sắc đến PGS.TS.Nguyễn Đức Bách tận tình giúp đỡ, hƣớng dẫn, quan tâm tạo điều kiện cho suốt trình học tập thực khóa luận tốt nghiệp Tôi xin trân trọng cảm ơn tất anh, chị, bạn bè làm việc môn tận tình giúp đỡ, đóng góp ý kiến bổ ích tạo điều kiện cho tơi hồn thành tốt khóa luận tốt nghiệp Mặc dù cố gắng hồn thiện luận văn nhiệt tình, lực mình, nhiên khơng thể tránh khỏi thiếu sót, mong nhận đƣợc đóng góp quý thầy bạn để tơi hồn thành khóa luận đƣợc tốt Tơi xin chân thành cảm ơn! Hà Nội, ngày… tháng… năm 2022 Sinh viên Phạm Văn Hào MỤC LỤC Formatted: Font color: Auto LỜI CAM ĐOAN LỜI CẢM ƠN MỤC LỤC DANH MỤC BẢNG 118 DANH MỤC BIỂU ĐỒ 129 DANH MỤC HÌNH 1510 DANH MỤC CHỮ VIẾT TẮT 1711 TÓM TẮT 1812 PHẦN I MỞ ĐẦU 2013 1.1 Đặt vấn đề 2013 1.2 Mục đích yêu cầu đề tài 2114 1.2.1 Mục đích 2114 1.2.2 Yêu cầu 2114 PHẦN II TỔNG QUAN TÀI LIỆU 2215 2.1 Giới thiệu vi tảo spirulina platensis 2215 2.1.1 Lịch sử phát triển vi tảo spirulina platensis 2215 2.1.2 Đặc điểm sinh học vi tảo Spirulina platensis 2316 2.1.2.1 Đặc điểm hình thái vi tảo Spirulina platensis 2316 2.1.2.2 Đặc điểm cấu tạo vi tảo Spirulina platensis 2517 2.1.2.3 Đặc điểm sinh sản vi tảo Spirulina platensis 2719 2.1.2.4 Đặc điểm sinh trƣởng vi tảo Spirulina platensis 2720 2.1.3 Các yếu tố ảnh hƣởng đến sinh trƣởng phát triển vi tảo Spirulina platensis 2921 Field Code Changed 2.1.3.1 Ảnh hƣởng nồng độ nitro 2921 2.1.3.2 Ảnh hƣởng tốc độ khuấy trộn, cƣờng độ chiếu sáng 3022 2.1.3.3 Ảnh hƣởng nguyên tố đa lƣợng vi lƣợng 3023 2.1.3.4 Ảnh hƣởng nồng độ kim loại môi trƣờng tới sinh trƣởng tảo 3225 2.2 Tình hình nghiên cứu tảo Spirulina platensis Việt Nam giới 3325 2.2.1 Tình hình nghiên cứu Việt Nam 3325 2.2.2 Tình hình nghiên cứu ni vi tảo S.platensis giới 3426 2.2 Tác dụng tảo xoắn Spirulina platensis khoáng chất tảo 3628 2.2.1 Tác dụng tảo xoắn Spirulina platensis 3628 2.2.2 Tác dụng khoáng chất tảo 3930 PHẦN III VẬT LIỆU VÀ PHƢƠNG PHÁP NGHIÊN CỨU 4234 3.1 Địa điểm thời gian thực 4234 3.2 Vật liệu nghiên cứu 4234 3.2.1 Chủng giống 4234 3.2.2 Hóa chất 4334 3.2.3 Máy móc thiết bị nghiên cứu 4436 3.3 Phƣơng pháp nghiên cứu 4637 3.3.1 Phƣơng pháp nuôi cấy 4637 3.3.2 Xác định hàm lƣợng chlorophyll, protein, phycocyanin 4737 3.4 Nội dung nghiên cứu 4841 3.4.1 Xây dựng mơi trƣờng thí nghiệm 4841 3.4.2 Tiến hành nuôi tảo môi trƣờng 5143 3.4.3 Thu hoạch tảo môi trƣờng sau nuôi cấy 5143 3.4.4 Xác định hàm lƣợng khoáng chất tảo thành phẩm 5244 3.4.4.1 Xác định hàm lƣợng sắt tảo 5345 3.4.4.1 Xác định hàm lƣợng kẽm tảo 5546 3.4.4.1 Xác định hàm lƣợng magie tảo 5748 PHẦN IV: KẾT QUẢ VÀ THẢO LUẬN 6450 4.1 Xây dựng đồ thị đƣờng chuẩn khoáng chất 6450 4.1.2 Xây dƣng đồ thị đƣờng chuẩn kẽm 6450 4.1.2 Xây dƣng đồ thị đƣờng chuẩn magie 6551 4.1.1 Sự ảnh hƣởng nồng độ FeSO4 môi trƣờng tới hàm lƣợng sắt tảo 6651 4.1.2 Sự ảnh hƣởng nồng độ ZnSO4 môi trƣờng tới hàm lƣợng kẽm tảo 6853 4.1.2 Sự ảnh hƣởng nồng độ MgSO4 môi trƣờng tới hàm lƣợng magie tảo 7054 4.2 Xây dựng đồ thị ảnh hƣởng nồng độ chất vô bổ sung tới hàm lƣợng hợp chất hữu tảo 7256 4.2.1 Sự ảnh hƣởng nồng độ FeSO4 bổ sung tới hàm lƣợng hợp chất hữu tảo 7256 4.2.2 Ảnh hƣởng nồng độ ZnSO4 bổ sung tới hàm lƣợng hợp chất hữu tảo 7558 4.2.2 Sự ảnh hƣởng nồng độ MgSO4 bổ sung tới hàm lƣợng hợp chất hữu tảo 7860 PHẦN V KẾT LUẬN VÀ ĐỀ NGHỊ 8262 5.1 Kết luận 8262 5.2 Đề nghị 8362 I Tài liệu tham khảo 8463 Error! Hyperlink reference not valid.LỜI CAM ĐOANError! defined.2 Bookmark not Field Code Changed Field Code Changed Error! Hyperlink reference not valid.LỜI CẢM ƠN Error! Bookmark not defined.3 Field Code Changed Error! Hyperlink reference not valid.MỤC LỤC Error! Bookmark not defined.4 Field Code Changed Error! Hyperlink reference not valid.DANH MỤC BẢNGError! defined.7 Bookmark not Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.DANH MỤC BIỂU ĐỒError! Bookmark not defined.8 Field Code Changed Error! Hyperlink reference not valid.DANH MỤC HÌNHError! defined.9 Field Code Changed Bookmark not Error! Hyperlink reference not valid.DANH MỤC CHỮ VIẾT TẮT Error! Bookmark not defined.10 Error! Hyperlink reference not valid.TÓM TẮT Error! Bookmark not defined.11 Error! Hyperlink reference not valid.PHẦN I MỞ ĐẦUError! defined.12 Error! Hyperlink reference not valid.1.1 Đặt vấn đềError! defined.12 Bookmark not Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Bookmark not Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.1.2 Mục đích yêu cầu đề tài Error! Bookmark not defined.13 Error! Hyperlink reference not valid.1.2.1 Mục đíchError! defined.13 Error! Hyperlink reference not valid.1.2.2 Yêu cầuError! defined.13 Bookmark not Field Code Changed Field Code Changed Field Code Changed Field Code Changed Bookmark not Field Code Changed Field Code Changed Error! Hyperlink reference not valid.PHẦN II TỔNG QUAN TÀI LIỆU Error! Bookmark not defined.14 Field Code Changed Error! Hyperlink reference not valid.2.1 Giới thiệu vi tảo spirulina platensis.Error! Bookmark not defined.14 Field Code Changed Error! Hyperlink reference not valid.2.1.1 Lịch sử phát triển vi tảo spirulina platensis Error! Bookmark not defined.14 Field Code Changed Error! Hyperlink reference not valid.Hình 2.1 Tảo Spirulina platensis Error! Bookmark not defined.15 Field Code Changed Error! Hyperlink reference not valid.2.1.2 Đặc điểm sinh học vi tảo Spirulina platensis Error! Bookmark not defined.15 Field Code Changed Error! Hyperlink reference not valid.2.1.2.1 Đặc điểm hình thái vi tảo Spirulina platensis Error! Bookmark not defined.15 Field Code Changed Error! Hyperlink reference not valid.2.1.2.2 Đặc điểm cấu tạo vi tảo Spirulina platensis Error! Bookmark not defined.17 Field Code Changed Error! Hyperlink reference not valid.2.1.2.3 Đặc điểm sinh sản vi tảo Spirulina platensis Error! Bookmark not defined.19 Field Code Changed Error! Hyperlink reference not valid.2.1.2.4 Đặc điểm sinh trƣởng vi tảo Spirulina platensis Error! Bookmark not defined.19 Field Code Changed Error! Hyperlink reference not valid.2.1.3 Các yếu tố ảnh hƣởng đến sinh trƣởng phát triển vi tảo Spirulina platensis Error! Bookmark not defined.20 Field Code Changed Error! Hyperlink reference not valid.2.1.3.1 Ảnh hƣởng nồng độ dinh dƣỡng Error! Bookmark not defined.20 Field Code Changed Error! Hyperlink reference not valid.2.2 Tình hình nghiên cứu tảo Spirulina platensis Việt Nam giới Error! Bookmark not defined.23 Field Code Changed Error! Hyperlink reference not valid.2.2.1 Tình hình nghiên cứu Việt Nam Error! Bookmark not defined.23 Field Code Changed Error! Hyperlink reference not valid.2.2.2 Tình hình nghiên cứu ni vi tảo S.platensis giới Error! Bookmark not defined.24 Field Code Changed Error! Hyperlink reference not valid.2.2 Tác dụng tảo xoắn Spirulina platensis khoáng chất tảo Error! Bookmark not defined.26 Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.2.2.1 Tác dụng tảo xoắn Spirulina platensis Error! Bookmark not defined.26 Field Code Changed Error! Hyperlink reference not valid.2.2.2 Tác dụng khoáng chất tảo Error! Bookmark not defined.29 Field Code Changed Error! Hyperlink reference not valid.PHẦN III VẬT LIỆU VÀ PHƢƠNG PHÁP NGHIÊN CỨU Error! Bookmark not defined.32 Field Code Changed Error! Hyperlink reference not valid.3.1 Địa điểm thời gian thực Error! Bookmark not defined.32 Field Code Changed Error! Hyperlink reference not valid.3.2 Vật liệu nghiên cứuError! Bookmark not defined.32 Field Code Changed Error! Hyperlink reference not valid.3.2.1 Chủng giốngError! defined.32 Field Code Changed Error! Hyperlink reference not valid.3.2.2 Hóa chấtError! defined.33 Bookmark not Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Bookmark not Field Code Changed Field Code Changed Error! Hyperlink reference not valid.Bảng 3.1 Môi trƣờng Zarrouk (Zarrouk, 1966) Error! Bookmark not defined.34 Field Code Changed Error! Hyperlink reference not valid.3.2.3 Máy móc thiết bị nghiên cứu Error! Bookmark not defined.35 Field Code Changed Error! Hyperlink reference not valid.Bảng 3.2 Các loại máy móc thiết bị nghiên cứu Error! Bookmark not defined.35 Field Code Changed Error! Hyperlink reference not valid.3.3 Phƣơng pháp nghiên cứu Error! Bookmark not defined.36 Field Code Changed Error! Hyperlink reference not valid.3.3.1 Phƣơng pháp nuôi cấy : Error! Bookmark not defined.36 Field Code Changed Error! Hyperlink reference not valid.3.3.2 Xác định hàm lƣợng chlorophyll, protein, phycocyanin Error! Bookmark not defined.36 Field Code Changed Error! Hyperlink reference not valid.3.4 Nội dung nghiên cứuError! not defined.37 Bookmark Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.3.4.1 Xây dựng mơi trƣờng thí nghiệm Error! Bookmark not defined.37 Field Code Changed Error! Hyperlink reference not valid.Bảng 3.3 Thống kê chất nồng độ thay đổi môi trƣờng thí nghiệm so với mơi trƣờng ban đầuError! Bookmark not defined.38 Field Code Changed Error! Hyperlink reference not valid.3.4.2 Tiến hành nuôi tảo môi trƣờng Error! Bookmark not defined.38 Field Code Changed Error! Hyperlink reference not valid.3.4.3 Thu hoạch tảo môi trƣờng sau nuôi cấy Error! Bookmark not defined.39 Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.3.4.4 Xác định hàm lƣợng khoáng chất tảo thành phẩm Error! Bookmark not defined.39 Field Code Changed Error! Hyperlink reference not valid.3.4.4.1 Xác định hàm lƣợng sắt tảo Error! Bookmark not defined.40 Field Code Changed Error! Hyperlink reference not valid.Bảng 3.4 công thức pha dung dịch xác định sắt Error! Bookmark not defined.41 Field Code Changed Error! Hyperlink reference not valid.3.4.4.1 Xác định hàm lƣợng kẽm tảo Error! Bookmark not defined.41 Field Code Changed Error! Hyperlink reference not valid.Bảng 3.5 công thức pha dung dịch xác định kẽm Error! Bookmark not defined.42 Field Code Changed Error! Hyperlink reference not valid.3.4.4.1 Xác định hàm lƣợng magie tảo Error! Bookmark not defined.43 Field Code Changed Error! Hyperlink reference not valid.Bảng 3.6 công thức pha dung dịch xác định magie Error! Bookmark not defined.43 Field Code Changed Error! Hyperlink reference not valid.3.4.5 Xác định hàm lƣợng protein tảo Error! Bookmark not defined.45 Field Code Changed Error! Hyperlink reference not valid.3.4.6 Xác định hàm lƣợng phycocyanin Error! Bookmark not defined.46 Field Code Changed Error! Hyperlink reference not valid.3.4.7 Xác định hàm lƣợng chlorophyll Error! Bookmark not defined.47 Field Code Changed Error! Hyperlink reference not valid.PHẦN IV: KẾT QUẢ VÀ THẢO LUẬN Error! Bookmark not defined.48 Field Code Changed Error! Hyperlink reference not valid.4.1 Xây dựng đồ thị tƣơng quan nồng độ chất vô bổ sung hàm lƣợng khoáng tảo Error! Bookmark not defined.48 Field Code Changed Error! Hyperlink reference not valid.4.1.1 Sự tƣơng quan nồng độ FeSO4 hàm lƣợng sắt tảo Error! Bookmark not defined.48 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.1 tƣơng quan nồng độ FeSO4 hàm lƣợng sắt tảo Error! Bookmark not defined.48 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.1 hàm lƣợng sắt tảo Error! Bookmark not defined.48 Field Code Changed Error! Hyperlink reference not valid.4.1.2 Sự tƣơng quan nồng độ ZnSO4 hàm lƣợng kẽm tảo Error! Bookmark not defined.49 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.2 tƣơng quan nồng độ ZnSO4 hàm lƣợng kẽm tảo Error! Bookmark not defined.49 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.2 hàm lƣợng kẽm tảo Error! Bookmark not defined.49 Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Field Code Changed Error! Hyperlink reference not valid.4.1.2 Sự tƣơng quan nồng độ MgSO4 hàm lƣợng magie tảo Error! Bookmark not defined.50 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.3 tƣơng quan nồng độ MgSO4 hàm lƣợng magie tảo Error! Bookmark not defined.50 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.3 hàm lƣợng magie tảo Error! Bookmark not defined.50 Field Code Changed Error! Hyperlink reference not valid.4.2 Xây dựng đồ thị tƣơng quan nồng độ chất vô bổ sung hàm lƣợng hợp chất hữu tảoError! Bookmark not defined.51 Field Code Changed Error! Hyperlink reference not valid.4.2.1 Sự tƣơng quan nồng độ FeSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.51 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.4 Tƣơng quan nồng độ FeSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.51 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.4 hàm lƣợng hợp chất hữu tảo bổ sung sắt Error! Bookmark not defined.52 Field Code Changed Error! Hyperlink reference not valid.4.2.2 Sự tƣơng quan nồng độ ZnSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.53 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.5 Tƣơng quan nồng độ ZnSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.53 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.5 Hàm lƣợng hợp chất hữu tảo bổ sung kẽm Error! Bookmark not defined.54 Field Code Changed Error! Hyperlink reference not valid.4.2.2 Sự tƣơng quan nồng độ MgSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.55 Field Code Changed Error! Hyperlink reference not valid.Bảng 4.6 Tƣơng quan nồng độ ZnSO4 bổ sung hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.55 Field Code Changed Error! Hyperlink reference not valid.Biểu đồ 4.6 hàm lƣợng hợp chất hữu tảo Error! Bookmark not defined.56 Field Code Changed Error! Hyperlink reference not valid.PHẦN V KẾT QUẢ VÀ ĐỀ NGHỊ Error! Bookmark not defined.57 Field Code Changed Error! 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Bookmark not defined.59 Field Code Changed tham khảo Field Code Changed Field Code Changed Field Code Changed Field Code Changed tăng hàm lƣợng FeSO4 mơi trƣờng tới nồng độ 0,222 µg l-1 ZnSO4 tảo ngƣng sinh trƣởng chết Hàm lƣợng Mg thay đổi lớn dao động từ 0,0057-0.0079 mg g tảo khô thu đƣợc => Khi bổ sung thêm hàm lƣợng MgSO4 lƣợng Mg tảo thu đƣợc tăng lên tới mức định dừng lại tiếp tục tăng thêm, ta tăng hàm lƣợng MgSO4 mơi trƣờng tới nồng độ 0,2 g/L MgSO4 tảo ngƣng sinh trƣởng chết Tùy thuộc vào hợp chất vô bổ sung hàm lƣợng hợp chất hữu tảo thay đổi theo hƣớng khác cụ thể : Khi thay đổi hàm lƣợng sắt hàm lƣợng chlorophyll có biến đổi lớn protein phycocyyanin có thay đổi nhƣng tần số thấp Formatted: Numbered + Level: + Numbering Style: 1, 2, 3, … + Start at: + Alignment: Left + Aligned at: 0.63 cm + Khi thay đổi hàm lƣợng Zn hàm lƣợng phycocyyanin có biến đổi lớn protein có thay đổi nhƣng tần số thấp chlorophyll có suy thối sớm Khi thay đổi hàm lƣợng Mg hàm lƣợng chlorophyll protein có biến đổi lớn phycocyyanin có thay đổi nhƣng tần số thấp Giới hạn chịu đựng tảo môi trƣờng chứa nồng độ ion kim loại cao nhiên nồng độ cao tảo sinh trƣởng chết 5.2 Đề nghị Dựa kết thu đƣợc lựa chọn mơi trƣờng có nồng độ hợp chất vô phù hợp để nuôi trồng tảo thành phẩm có hàm lƣợng khống hợp chất hữu mong muốn cho mục đích khác nhaulà 0,05 g/l FeSO4, 1,11 µg/l ZnSO4 0,1 g/l MgSO4 để đạt đƣợc hàm lƣợng khoáng mong muốn tối đa 84 Formatted: List Paragraph, Line spacing: 1.5 lines, Numbered + Level: + Numbering Style: 1, 2, 3, … + Start at: + Alignment: Left + Aligned at: 0.63 cm + Indent at: 1.27 cm I PHẦN VI.TÀI LIỆU THAM KHẢO M A Kean, E Brons Delgado, B.P Mensink, M.H.J Bugter(2015) Iron chelating agents and their effects on the growth of Pseudokirchneriella subcapitata, Chlorella vulgaris, Phaeodactylum tricornutum and Spirulina platensis in comparison to Fe- EDTA Mostafa M.S Ismaiel, Michele D Piercey-Normorea, Christof Rampitsch(2018) Proteomic analyses of the cyanobacterium Spirulina (Spirulina) platensis under iron and salinity stress 85 Formatted: Heading 1, Centered, No bullets or numbering Formatted: Font: 13 pt, Not Bold, Italic, English (U.S.) Formatted: Font: 13 pt, Not Bold, Italic, English (U.S.) Formatted: Font: 13 pt, Not Bold, Italic, English (U.S.) Izabela Michalaka, Małgorzata Mironiuka, Katarzyna Godlewskab, Justyna Tryndac, Krzysztof Marycz(2017) Spirulina (Spirulina) platensis: An effective biosorbent for nutrients Ting Zhoua, Jingjing Wanga, Hongli Zhenga, Xiaodan Wua, Yunpu Wanga, Mingzhi Liua, Shuyu Xianga, Leipeng Caoa, Roger Ruana,b, Yuhuan Liu(2018)Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis J A LoneJ A & A Kumar & S Kundu & F A LoneF A & M R Suseela (2013)Characterization of Tolerance Limit in Spirulina platensis in Relation to Nanoparticles Akbarnezhad, Shamsaie Mehrgan, Kamali, Javaheri Baboli (2019)Effects of microelements (Fe, Cu, Zn) on growth and pigment contents of Spirulina (Spirulina) platensis Guillaume Cogne,1 Bernd Lehmann,2 Claude-Gilles Dussap,1 Jean-Bernard Gros (2002) Uptake of Macrominerals and Trace Elements by the Cyanobacterium Spirulina platensis (Spirulina platensis PCC 8005) Under Photoautotrophic Conditions: Culture Medium Optimization Merve Esen1 Raziye Ozturk Urek(2011) Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis Tapan Adhikari, S Kundu, Ajay A K Biswas, J C TarafdarJ C & A Subba Rao (2014) Effect of Zinc, Copper and Calcium Phosphate Nano Particles on Growth of Spirulina platensis 10 Ting Zhou, Xuan Lia, Qi Zhang, Shiman Dong, Huan Liu, Yuhuan Liu, Alex V.Chaves, Peter J.Ralphe, Roger Ruan, Qilin Wang (2022) Ecotoxicological response of Spirulina platensis to coexisted copper and zinc in anaerobic digestion effluent 11 TingZhou, JingjingWang, HongliZheng, XiaodanWu, YunpuWang, MingzhiLiu, ShuyuXiang, LeipengCao, RogerRuan, YuhuanLiu(2018)Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis 12 Inga Zinicovscaia, Liliana Cepoi, Ludmila Rudi, Tatiana Chiriac, Dmitrii Grozdov(2021) Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition 13 A A NalimovaA A., V V PopovaV V., L N TsoglinL N (2005) The effects of copper and zinc on Spirulina platensis growth and heavy metal accumulation in its cells 14 Sinouvassane Djearamane, Yang Mooi Lim, Ling Shing Wong, Poh Foong Lee (2018) Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Spirulina) platensis 15 Mostafa M S Ismaiel, Yassin M El-Ayouty, Peter C Loewen (2014) Characterization of the iron-containing superoxide dismutase and its response to stress in cyanobacterium Spirulina (Spirulina) platensis 86 16 Merve Esen Raziye Öztürk Ürek (2014) Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis 17 Sarika Saxena, Monika Saxena, Mukul Saxena, Manish Pratap Singh, Dr Ashish Kumar Saxena(2012) Differential modification of growth by iron in Anabaena cylindrica and Spirulina platensis 18 Raziye Ozturk Urek, Leman Tarhan(2011)Effect of Iron Deficiency and Supplemented Conditions on the Antioxidant System, Membrane Lipid Peroxidation and Some Metal Levels in Spirulina maxima 19 TingZhou, XuanLi, QiZhang, ShimanDong, HuanLiu, Yuhuan Liu bAlex V.Chaves, Peter J.Ralph, RogerRuan, QilinWanga (2022)Ecotoxicological response of Spirulina platensis to coexisted copper and zinc in anaerobic digestion effluent 20 TingZhou, XuanLi, QiZhang, ShimanDong, HuanLiu, Yuhuan Liu, QilinWanga (2018)Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis 21 Inga Zinicovscaia, Liliana Cepoi, Ludmila Rudi, Tatiana Chiriac, Dmitrii Grozdov(2021) Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition 22 A A Nalimova, V V Popova, L N Tsoglin(2005) The effects of copper and zinc on Spirulina platensis growth and heavy metal accumulation in its cells 23 Sinouvassane Djearamane, Yang Mooi Lim, Ling Shing Wong, Poh Foong Lee(2018) Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Spirulina) platensis 24 Mostafa M S Ismaiel, Yassin M El-Ayouty, Peter C Loewen(2014) Characterization of the iron-containing superoxide dismutase and its response to stress in cyanobacterium Spirulina (Spirulina) platensis 25 MERVE ESEN, RAZİYE ÖZTÜRK ÜREK(2014) Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis 26 Sarika Saxena, Monika Saxena, Mukul Saxena, Manish Pratap Singh, Dr Ashish Kumar Saxena(2012) Differential modification of growth by iron in Anabaena cylindrica and Spirulina platensis 27 Raziye Ozturk Urek and Leman Tarhan(2011) Effect of Iron Deficiency and Supplemented Conditionson the Antioxidant System, Membrane Lipid Peroxidationand Some Metal Levels in Spirulina maxima 28 Leyla Uslu , Oya Iik, Kemal Koỗ, Tolga Gửksan(2011) The effects of nitrogen deficiencies on the lipid and protein contents of Spirulina platensis 29 Merve Esen,Raziye Ozturk Urek(2014) Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis 30 M A Kean , E Brons Delgado, B.P Mensink, M.H.J Bugter(2015) Iron chelating agents and their effects on the growth of Pseudokirchneriella subcapitata, Chlorella vulgaris, Phaeodactylum tricornutum and Spirulina platensis in comparison to Fe- EDTA 87 31 Grộgoire Puyfoulhoux, Jean-Max Rouanet, Pierre Besanỗon, Bruno Baroux, JeanClaude Baccou, and Bertrand Caporiccio(2001) Iron Availability from IronFortified Spirulina by an in Vitro Digestion/Caco-2 Cell Culture Model 32 Samy Boussiba, Amos E Richmond(1980) C-phycocyanin as a storage protein in the blue-green alga Spirulina platensis 33 Hanaa adb el baky(2003) Over Production of Phycocyanin Pigment in Blue Green Alga Spirulina sp and It`s Inhibitory Effect on Growth of Ehrlich Ascites Carcinoma Cells 34 Alfredo Walter, Júlio Cesar de Carvalho, Vanete Thomaz Soccol, Ana Bárbara Bisinella de Faria, Vanessa Ghiggi, Carlos Ricardo Soccol (2011)Study of phycocyanin production from Spirulina platensis under different light spectra 35 Feng Chen, Yiming Zhang, Siyuan Guo(1996) Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture 36 Maryam Akbarnezhad, Mehdi Shamsaie Mehrgan, Abolghasem kamali, Mehran Javaheri Baboli(2019) Effects of microelements (Fe, Cu, Zn) on growth and pigment contents of Spirulina (Spirulina) platensis 37 Nidhi Raj Yadav(2015) Toxic Effect of Chlorpyrifos and Dimethoate on Protein and Chlorophyll-a Content of Spirulina Platensis 38 Saadet Demirors, Saygideger, Ozlem Okkay(2008) Effect of 2,4Dichlorophenoxyacetic acid on growth, protein and chlorophyll-a content of Chlorella vulgaris and Spirulina platensis cells 39 Sundaramoorthy Balaji,Thiagarajan Kalaivani,Chandrasekaran Rajasekaran(2013) Biosorption of Zinc and Nickel and Its Effect on Growth of Different Spirulina Strains 40 GilverRosero-Chasoy, Rosa M.Rodríguez-Jasso, Cristóbal N.Aguilara, GermánBuitrón, IsaacChairez, Héctor A.Ruiz(2022) Growth kinetics and quantification of carbohydrate, protein, lipids, and chlorophyll of Spirulina platensis under aqueous conditions using different carbon and nitrogen sources 41 Carlota de Oliveira, Rangel-Yagui, Eliane Dalva Godoy, Danesi, João Carlos Monteirode Carvalho, SunaoSato(2004) Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process 42 E.D.GDanesi, Cde O Rangel-Yagui, J.C.Mde Carvalho, S Sato(2002) An investigation of effect of replacing nitrate by urea in the growth and production of chlorophyll by Spirulina platensis 43 SondaBenelhadj, AdemGharsallaoui, PascalDegraeve, HamadiAttia, DorraGhorbel(2016) Effect of pH on the functional properties of Spirulina (Spirulina) platensis protein isolate 44 Etiele Greque de Morais, Itaciara Larroza Nunes, Janice Izabel Druzian, Michele Greque de Morais, Ana Priscila Centeno da Rosa Jorge Alberto Vieira Costa(2020) Increase in biomass productivity and protein content of Spirulina sp LEB 18 (Spirulina) cultivated with crude glycerol 88 45 Trần Anh Tuấn, Nguyễn Xuân Việt , Phan Thị Yến , Hoàng Thị Phƣơng Thúy(2020) NGHIÊN CỨU CẢI TIẾN MÔI TRƢỜNG NUÔI CẤY TẢO XOẮN (Athrospira platensis) QUY MƠ PHÕNG THÍ NGHIỆM 46 Nada K Alharbi, Mayasar I Al-Zaban, Fawziah M Albarakaty, Sayed F Abdelwahab(2022) Kinetic, Isotherm and Thermodynamic Aspects of Zn2+ Biosorption by Spirulina platensis: Optimization of Process Variables by Response Surface Methodology 47 Gabriel Luis Castiglioni, Fernanda Ferreira Freitas, Celso JosédeMoura, Marco Antônio Assfalk de Oliveira(2021) Biosorption study of magnesium, zinc, iron and selene in Spirulina platensis high concentration crops 48 Lívia SenoFerreira, Mayla SantosRodriguesn Jỗo Carlos Monteirode Carvalho(2011) Adsorption of Ni2+, Zn2+ and Pb2+ onto dry biomass of Spirulina (Spirulina) platensis and Chlorella vulgaris I Single metal systems 49 Meysam Azadian,Hossein Ghanadzadeh Gilani,Hossein Rajabi-Kuyakhi,Nazanin Gharibipour(2021) Kinetic, isotherm, thermodynamic, and adsorption capacity studies of magnetic Spirulina microalgae onto zinc (II) 50 Arthit Jatupornpongchai (2013)ENRICHMENT OF ORGANIC ZINC IN SPIRULINA PLATENSIS 51 Principe, María VirginiaIcon ; Permigiani, Isis SabrinaIcon , Della Vedova, Maria CeciliaIcon , Petenatti, Elisa Margarita; Pacheco, Pablo HugoIcon , Gil, Raul Andres(2020) Bioaccessibility studies of Fe, Cu and Zn from Spirulina dietary supplements with different excipient composition and dosage form 52 Cepoi Liliana, Valuţă Ana, Doni Veronica, Spỵnu Crina, Dumbrăveanu Veronica, Rudic Valeriu(2019) The action of Zn(II) acetate on adaptive capacity of spirulina in response to changes in the light regime 53 Sinouvassane Djearamane, Yang Mooi Lim, Ling Shing Wong, Poh Foong Lee(2018) Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Spirulina) platensis 54 E Gelagutashvili, N.Bagdavadze, A.Gongadze, M.Gogebashvili, N.Ivanishvili(2021) Effect of Ag(I), Ni(II), Zn(II) ions on Irradiated Spirulina platensis 55 Sundaramoorthy Balaji,Thiagarajan Kalaivani,Chandrasekaran Rajasekaran(2013) Biosorption of Zinc and Nickel and Its Effect on Growth of Different Spirulina Strains 56 Mona Kaamoush, Nagwa El-Agawany, Hamida El Salhin & Ahmed ElZeiny(2022) Monitoring effect of nickel, copper, and zinc on growth and photosynthetic pigments of Spirulina platensis with suitability investigation in Idku Lake 57 Inga Zinicovscaia, Liliana Cepoi, Ludmila Rudi, Tatiana Chiriac, Dmitrii Grozdov(2021) Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition 89 58 Nalimova, V V Popova, L N Tsoglin & N A Pronina(2005) The effects of copper and zinc on Spirulina platensis growth and heavy metal accumulation in its cells 59 TingZhou, XuanLi, QiZhang, ShimanDong, HuanLiu, Yuhuan Liu, QilinWanga (2018)Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis 60 Mostafa M S Ismaiel, Yassin M El-Ayouty, Peter C Loewen & Michele D Piercey-Normore Journal of Applied Phycology(2014) Characterization of the iron-containing superoxide dismutase and its response to stress in cyanobacterium Spirulina (Spirulina) platensis 61 Mostafa M.S.Ismaiel, Michele D.Piercey-Normore, Christof Rampitschc(2018) Proteomic analyses of the cyanobacterium Spirulina (Spirulina) platensis under iron and salinity stress 62 Sarika Saxena, Monika Saxena, Mukul Saxena, Manish Pratap Singh, Dr Ashish Kumar Saxena(2012) Differential modification of growth by iron in Anabaena cylindrica and Spirulina platensis 63 Su Peng Loh, Hishamuddin Omar, Abdul Salam Abdullahl, Maznah Ismail(2006) Effects of calcium supplementation on iron bioavailability from spirulina 64 Yurii D.Perfiliev, Alexandr Kh.Tambiev, Maxim A.Konnychev, Anatoly V.Skalny, Elena S.Lobakova, Mikhail P.Kirpichnikov(2018) Mössbauer spectroscopic study of transformations of iron species by the cyanobacterium Spirulina platensis (formerly Spirulina platensis) 65 Maryam Akbarnezhad, Mehdi Shamsaie Mehrgan, Abolghasem kamali, Mehran Javaheri Baboli(2020) Effects of microelements (Fe, Cu, Zn) on growth and pigment contents of Spirulina (Spirulina) platensis 66 RUDIC, Valeriu; BULIMAGA, Valentina; ZOSIM, Lililana; CHIRIAC, Tatiana; TURTA, C.; LAZARESCU, Ana (2005) Nanobiotechnologies of iron rich spirulina cultivation as a source of antianemic products 67 Akbarnezhad, Maryam, Mehrgan, Mehdi Shamsaie, Kamali, Abolghasem; Baboli, Mehran Javaheri(2016) Bioaccumulation of Fe+2 and its effects on growth and pigment content of spirulina (Spirulina platensis) 68 SilviaMelnic, DenisProdius, CharlesSimmons, LilianaZosim, TatianaChiriac, ValentinaBulimaga, ValeriuRudic, ConstantinTurtaa(2011) Biotechnological application of homo- and heterotrinuclear iron(III) furoates for cultivation of ironenriched Spirulina 69 Naeini F, Zarezadeh M, Mohiti S, Tutunchi H, Ebrahimi Mamaghani M, Ostadrahimi A Spirulina supplementation as an adjuvant therapy in enhancement of antioxidant capacity: a systematic review and meta-analysis of controlled clinical trials Int J Clin Pract 2021;75(10):e14618 70 70.Machowiec P, Ręka G, Maksymowicz M, Piecewicz-Szczęsna H, Smoleń A Effect of spirulina supplementation on systolic and diastolic blood pressure: 90 systematic review and meta-analysis of randomized controlled trials Nutrients 2021;13(9):3054 71 Hamedifard Z, Milajerdi A, Reiner Ž, Taghizadeh M, Kolahdooz F, Asemi Z The effects of spirulina on glycemic control and serum lipoproteins in patients with metabolic syndrome and related disorders: a systematic review and meta-analysis of randomized controlled trials Phytother Res 2019;33(10):2609-2621 72 Serban MC, Sahebkar A, Dragan S, et al A systematic review and meta-analysis of the impact of Spirulina supplementation on plasma lipid concentrations Clin Nutr 2016;35(4):842-851 73 Mazokopakis EE, Papadomanolaki MG, Fousteris AA, Kotsiris DA, Lampadakis IM, Ganotakis ES The hepatoprotective and hypolipidemic effects of Spirulina (Spirulina platensis) supplementation in a Cretan population with non-alcoholic fatty liver disease: a prospective pilot study Ann Gastroenterol 2014;27(4):387394 74 Yousefi R, Saidpour A, Mottaghi A The effects of Spirulina supplementation on metabolic syndrome components, its liver manifestation and related inflammatory markers: a systematic review Complement Ther Med 2019;42:137-144 75 Moradi S, Ziaei R, Foshati S, Mohammadi H, Nachvak SM, Rouhani MH Effects of Spirulina supplementation on obesity: a systematic review and meta-analysis of randomized clinical trials Complement Ther Med 2019;47:102211 76 C Windsor, MPH, RDN, R (2022, October 10) Spirulina: Uses, Benefits, Side Effects, Dosage, Precautions Verywell Health 77 77.National Institutes Of Health (2021) Office of Dietary Supplements - Iron Nih.gov 78 National Institutes of Health (2016a) Office of Dietary Supplements Magnesium Nih.gov 79 National Institutes of Health (2016b) Office of Dietary Supplements - Zinc Nih.gov Formatted: Normal, No bullets or numbering, Tab stops: Not at 1.9 cm Patel A., Mishra S., Pawar R., Ghosh P.K (2005) Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat Protein Expression and Purification, 40, pp.248-255 M A Kean M A., E Brons Delgado E B., B.P Mensink B P., M.H.J Bugter M H J (2015) Iron chelating agents and their effects on the growth of Pseudokirchneriella subcapitata, Chlorella vulgaris, Phaeodactylum tricornutum and Spirulina platensis in comparison to Fe- EDTA Journal of Algal Biomass Utilization, 6(1): 56-73 Mostafa M.S Ismaiel M M S., Michele D Piercey-Normorea M D., Christof Rampitsch C (2018) Proteomic analyses of the cyanobacterium Spirulina 91 Formatted: Left Formatted: Font color: Red Formatted: Font color: Red Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic (Spirulina) platensis under iron and salinity stress https://doi.org/10.1016/j.envexpbot.2017.11.013 Izabela Michalaka I., Małgorzata Mironiuka M., Katarzyna Godlewskab K., Justyna Tryndac J., Krzysztof Marycz K (2017) Spirulina (Spirulina) platensis: An effective biosorbent for nutrients https://doi.org/10.1016/j.procbio.2019.10.004 Ting Zhoua T., Jingjing Wanga T., Hongli Zhenga H., Xiaodan Wua X., Yunpu Wanga Y., Mingzhi Liua M., Shuyu Xianga S., Leipeng Caoa L., Roger Ruana R.,,b, Yuhuan Liu Y (2018) Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis.https://doi.org/10.1016/j.biortech.2018.08.131 J A Lone J A , & A Kumar A &, S Kundu S &, F A Lone F A & M R Suseela M R (2013) Characterization of Tolerance Limit in Spirulina platensis in Relation to Nanoparticles Water Air Soil Pollut 224, 1670 (2013) https://doi.org/10.1007/s11270-013-1670-6 Akbarnezhad M., Shamsaie MehrganM M., Kamali A., Javaheri B M Baboli (202019) Effects of microelements (Fe, Cu, Zn) on growth and pigment contents of Spirulina (Spirulina) platensis.Iranian Journal of Fisheries Sciences, 19(2) 653668 DOI: 10.22092/ijfs.2019.120614 Cogne G., Lehmann B., Dussap C G., Gros J B (2003) Uptake of macrominerals and trace elements by the cyanobacterium Spirulina platensis (Arthrospira platensis PCC 8005) under photoautotrophic conditions: culture medium optimization Biotechnol Bioeng; 81(5):588-93 doi: 10.1002/bit.10504 Guillaume Cogne,1 Bernd Lehmann,2 Claude-Gilles Dussap,1 Jean-Bernard Gros (2002) Uptake of Macrominerals and Trace Elements by the Cyanobacterium Spirulina platensis (Spirulina platensis PCC 8005) Under Photoautotrophic Conditions: Culture Medium Optimization Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Esen M., Ozturk U R (2015) Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis Biotechnol Appl Biochem; 62(2):275-86 doi: 10.1002/bab.1268.Merve Esen1 Raziye Ozturk Urek(2011) Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis 10 Tapan Adhikari T., S Kundu S., Ajay A K Biswas A A K., J C Tarafdar J C & A Subba Rao A S (2014) Effect of Zinc, Copper and Calcium Phosphate Nano Particles on Growth of Spirulina platensis National Academy Science Letter; 37, 207-212 https://doi.org/10.1007/s40009-014-0223-x 11 Ting Zhou T., Xuan Lia X., Qi Zhang Q., Shiman Dong S., Huan Liu H., Yuhuan Liu Y., Alex V.Chaves A V., Peter J.Ralphe P J., Roger Ruan R., Qilin Wang Q (2022) Ecotoxicological response of Spirulina platensis to coexisted copper and zinc in anaerobic digestion effluent Science of The Total Environment, Volume 7, Article 155874 https://doi.org/10.1016/j.scitotenv.2022.155874.Ti 92 Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic 12 Inga Zinicovscaia I., Liliana Cepoi L., Ludmila Rudi L., Tatiana Chiriac T., Dmitrii Grozdov D (2021) Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition Environmental Science and Pollution Research, 28, 52216-52224 https://doi.org/10.1007/s11356021-14457-6 13 A A Nalimova A A., V V Popova V V., L N Tsoglin L N (2005) The effects of copper and zinc on Spirulina platensis growth and heavy metal accumulation in its cells Russian Journal of Plant Physiology, Volume 52, Issue 2, Pages 229-234 https://doi.org/10.1007/s11183-005-0035-4 14 Sinouvassane Djearamane S., Yang Mooi Lim Y M., Ling Shing Wong L S., Poh Foong Lee P F (2018) Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Spirulina) platensis doi: 10.7717/peerj.4682 15 Mostafa M S Ismaiel M M S., Yassin M El-Ayouty Y M., Peter C Loewen P C., Percey-Normore M D (2014) Characterization of the iron-containing superoxide dismutase and its response to stress in cyanobacterium Spirulina (Spirulina) platensis Journal of Applied Phycology, 26, 1649-1658 https://doi.org/10.1007/s10811-013-0233-y 16 Merve Esen M., Raziye Öztürk Ürek R Ö (2014) Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis Turkish Journal of Biology DOI: 10.3906/biy-1405-37 17 Sarika Saxena S., Monika Saxena M., Mukul Saxena M., Manish Pratap Singh M P., Dr Ashish Kumar Saxena A K (2012) Differential modification of growth by iron in Anabaena cylindrica and Spirulina platensis Journal of Pharmacy Research, 5(6), 3033-3037 Raziye Ozturk Urek R O., Leman Tarhan L (2011) Effect of Iron Deficiency and Supplemented Conditions on the Antioxidant System, Membrane Lipid Peroxidation and Some Metal Levels in Spirulina maxima Journal of Pure & Applied Microbiology, 5(2), 593-602 TingZhou, XuanLi, QiZhang, ShimanDong, HuanLiu, Yuhuan Liu bAlex V.Chaves, Peter J.Ralph, RogerRuan, QilinWanga (2022)Ecotoxicological response of Spirulina platensis to coexisted copper and zinc in anaerobic digestion effluent 18 TingZhou, XuanLi, QiZhang, ShimanDong, HuanLiu, Yuhuan Liu, QilinWanga (2018)Characterization of additional zinc ions on the growth, biochemical composition and photosynthetic performance from Spirulina platensis Inga Zinicovscaia, Liliana Cepoi, Ludmila Rudi, Tatiana Chiriac, Dmitrii Grozdov(2021) Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition A A Nalimova, V V Popova, L N Tsoglin(2005) The effects of copper and zinc on Spirulina platensis growth and heavy metal accumulation in its cells Sinouvassane Djearamane, Yang Mooi Lim, Ling Shing Wong, Poh Foong Lee(2018) Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Spirulina) platensis 93 Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Mostafa M S Ismaiel, Yassin M El-Ayouty, Peter C Loewen(2014) Characterization of the iron-containing superoxide dismutase and its response to stress in cyanobacterium Spirulina (Spirulina) platensis MERVE ESEN, RAZİYE ÖZTÜRK ÜREK(2014) Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis Sarika Saxena, Monika Saxena, Mukul Saxena, Manish Pratap Singh, Dr Ashish Kumar Saxena(2012) Differential modification of growth by iron in Anabaena cylindrica and Spirulina platensis Raziye Ozturk Urek and Leman Tarhan(2011) Effect of Iron Deficiency and Supplemented Conditionson the Antioxidant System, Membrane Lipid Peroxidationand Some Metal 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