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AL225Asubstitutioninthehumantumoursuppressor HIC1
abolishes itsinteractionwiththecorepressor CtBP
Nicolas Stankovic-Valentin
1,
*, Alexis Verger
2
, Sophie Deltour-Balerdi
1,†
, Kate G. R. Quinlan
2
,
Merlin Crossley
2
and Dominique Leprince
1,
*
1 CNRS UMR 8526, Institut de Biologie de Lille, Institut Pasteur de Lille, France
2 School of Molecular and Microbial Biosciences, University of Sydney, New South Wales, Australia
HIC1 (hypermethylated in cancer 1) encodes a tran-
scriptional repressor and is located in 17p13.3 in a
region frequently hypermethylated or deleted in many
types of prevalent humantumour [1]. HIC1 is a
tumour suppressor gene, since heterozygous HIC1
+ ⁄ –
mice develop, after 70 weeks, a gender-dependent spec-
trum of spontaneous malignant tumours [2]. HIC1 is a
direct target gene of P53 [1,3,4]. Moreover, elegant
animal models using Hic1 and p53 double heterozy-
gous knockout mice have shown that the epigenetically
silenced gene, Hic1, cooperates withthe mutated
tumour suppressor gene Trp53 in determining cancer
Keywords
17p13.3; CtBP; HIC1; Miller–Dieker
syndrome; transcriptional repression
Correspondence
D. Leprince, CNRS UMR 8161, Institut de
Biologie de Lille, Institut Pasteur de Lille,
1 Rue Calmette, 59017 Lille Cedex, France
Fax: +33 3 20 87 1111
Tel: +33 3 20 87 1119
E-mail: dominique.leprince@ibl.fr
Present address
*CNRS UMR 8161, Institut de Biologie de
Lille, Institut Pasteur de Lille, France
†Wellcome Trust, University of Cambridge,
UK
(Received 3 March 2006, revised 27 April
2006, accepted 2 May 2006)
doi:10.1111/j.1742-4658.2006.05301.x
HIC1 (hypermethylated in cancer) is atumoursuppressor gene located in
17p13.3, a region frequently hypermethylated or deleted in many types of
prevalent human tumour. HIC1 is also a candidate for a contiguous-gene
syndrome, the Miller–Dieker syndrome, a severe form of lissencephaly
accompanied by developmental anomalies. HIC1 encodes a BTB ⁄ POZ-zinc
finger transcriptional repressor. HIC1 represses transcription via two auton-
omous repression domains, an N-terminal BTB ⁄ POZ and a central region,
by trichostatin A-insensitive and trichostatin A-sensitive mechanisms,
respectively. TheHIC1 central region recruits thecorepressorCtBP (C-ter-
minal binding protein) through a conserved GLDLSKK motif, a variant of
the consensus C-terminal binding protein interaction domain PxDLSxK ⁄ R.
Here, we show that HIC1 interacts with both CtBP1 and CtBP2 and that
this interaction is stimulated by agents increasing NADH levels. Further-
more, point mutation of two CtBP2 residues forming part of the structure
of the recognition cleft for a PxDLS motif also ablates theinteraction with
a GxDLS motif. Conversely, in perfect agreement withthe structural data
and the universal conservation of this residue in all C-terminal binding pro-
tein-interacting motifs, mutation of the central leucine residue (leucine 225
in HIC1) abolishestheinteraction between HIC1 and CtBP1 or CtBP2. As
expected from thecorepressor activity of CtBP, this mutation also impairs
the HIC1-mediated transcriptional repression. These results thus demon-
strate a strong conservation inthe binding of C-terminal binding protein-
interacting domains despite great variability in their amino acid sequences.
Finally, this L225A point mutation could also provide useful knock-in ani-
mal models to study the role of the HIC1–CtBP interactionin tumorigenesis
and in development.
Abbreviations
AD, activation domain; CID, CtBP-interacting domain; CR, central region; CtBP, C-terminal binding protein; DBD, DNA-binding domain; EMT,
epithelial-to-mesenchymal transition; HDAC, histone deacetylase; HPE, holoprosencephaly; KI, knock-in; MDS, Miller–Dieker syndrome; TSA,
trichostatin A.
FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS 2879
progression and spectrum [5]. Finally, a circular regu-
latory loop has been proposed for HIC1, SIRT1 and
p53, since HIC1 represses the transcription of SIRT1,
SIRT1 deacetylates P53, thus negatively modulating
its DNA-binding properties, and P53 transactivates
HIC1 [6].
Besides its role in tumorigenesis, HIC1 is essential
during development and is also a candidate for a conti-
guous-gene syndrome, the Miller–Dieker syndrome
(MDS), a severe form of lissencephaly accompanied by
developmental anomalies [7]. HIC1 is located within the
critical 350 kbp region deleted in most patients [8], and
together with perinatal death and a reduction in overall
size, Hic1
– ⁄ –
mouse embryos have many developmental
defects resembling those found in MDS patients [9]. In
addition, parts of Hic1 expression territories in mice
embryos and in zebrafish strikingly overlap with regions
that exhibit abnormalities in MDS patients, e.g. cranio-
facial and limb mesenchymes [10,11].
The HIC1 protein is a sequence-specific transcrip-
tional repressor containing three main functional
domains: a conserved protein–protein interaction
domain called BTB⁄ POZ at the N-terminus, five
Kru
¨
ppel-like C
2
H
2
zinc fingers near its C-terminus, and
a central region, which is not well-conserved among
the HIC1 and the paralogous HRG22 proteins from
various species (Fig. 1) [12–14]. HIC1 binds specifically
to DNA through its zinc finger domain, which recogni-
zes the recently defined consensus sequence
5¢-
C
⁄
G
NG
C
⁄
G
GGGCA
C
⁄
A
CC-3¢ [15]. The BTB ⁄ POZ
domain is a conserved structural motif found mainly
in transcription factors, actin-binding proteins and
substrate-specific adapters of CUL-3-based ubiquitin
ligases [16]. The BTB ⁄ POZ domain is essential for the
function of transcriptional repressors by directly
recruiting nuclear corepressor (SMRT, N-CoR or
B-CoR)–histone deacetylase complexes (HDACs), as
shown for thehuman PLZF and BCL6 proteins
[17,18]. Previously, we have shown that theHIC1 and
HRG22 BTB ⁄ POZ domains are autonomous trans-
criptional repression domains, unable to recruit class I
or II HDACs, since they are insensitive to the specific
inhibitor trichostatin A (TSA) [12,13]. The HIC1
central region is also an autonomous transcriptional
repression domain, which recruits the corepressor
C-terminal binding protein 1 (CtBP1) [19,20] and
represses transcription ina TSA-sensitive manner [14].
Notably, HIC1 recruits CtBP1 through a short phylo-
genetically conserved sequence, GLDLSKK, slightly
divergent from the canonical CtBP-interacting domain
(CID) containing a PxDLSxK ⁄ R motif found in virtu-
ally all CtBP-interacting proteins [11,14,20].
The vertebrate genomes contain two different genes,
CtBP1 and CtBP2, widely expressed in normal human
tissues and cancer cell lines as well as throughout
development in mice [21]. These genes encode at least
four protein isoforms, including the corepressors
CtBP1 and CtBP2. These two proteins have partially
redundant functions. For example, during murine
development, Ctbp1 is poorly detectable inthe extra-
embryonic structures that express Ctbp2. Furthermore,
mouse Ctbp2
– ⁄ –
embryos die between E9 and E10.5
due to defects, notably in formation of the placenta
and neural ectoderm [21]. In contrast, Ctbp1
– ⁄ –
mice
are viable although they exhibit reduced fitness
and fertility [21]. CtBP1 is both cytoplasmic and nuc-
lear, and this subcellular localization is regulated by
interplay between post-transcriptional modifications
(SUMOylation and phosphorylation) and binding to
neural nitric oxide synthase, a PDZ domain-containing
protein [22,23]. In contrast, CtBP2 is mainly nuclear,
due to a specific N-terminal 20 amino acid region
containing Lys residues acetylated by P300 [24,24a].
Finally, CtBP1 is SUMOylated by PIAS1 and PIASxb
on Lys428, which is not conserved in CtBP2 [23],
whereas SUMOylation of CtBP2 on a nonconsensus
targeting motif requires the presence of Pc2 as an E3
ligase [25].
In this article, we demonstrate that HIC1 can inter-
act with both CtBP1 and CtBP2. This interaction relies
on a GxDLS motif that is slightly divergent from the
PxDLS consensus motif found in most CtBP-interact-
ing proteins. Furthermore, point mutation of two
CtBP2 residues forming part of the structure of the
recognition cleft for a PxDLS motif also ablates the
PLCGLDLSKKSPPGSAAP
PLCGLDLSKKSPPGSSVP
PPCGLDLSKKSPTGPSAQ
SVYGLDLSKKSPNSQSQL
PNYGLDLSKKSPSPNSQT
*********
Hu HIC1
714
BTB/POZ
Hu HIC1
Mu HIC1
Ck
FBPB
Zf HIC1b
Fu HIC1
Cons
1
Zinc Fingers
225
CID
135 422
Fig. 1. Schematic drawing of thehumanHIC1 protein. The
BTB ⁄ POZ domain and the five C
2
H
2
zinc fingers are represented as
dotted and grey boxes, respectively. The evolutionarily conserved
CtBP interaction domain (CID) [14] is represented as a dotted line,
and its sequence inthe various HIC1 proteins is shown. The central
Leu (residue 225 inhuman HIC1), which is the sole invariant resi-
due in all the CID motifs described so far, is highlighted in bold. In
the consensus lane (Cons), identical residues are indicated by *
under the aligned sequences. Hu, Human; Mu, Murine; Ck,
Chicken; Zf, zebrafish (Danio rerio); Fu, Fugu rubipres.
HIC1 interacts withCtBP N. Stankovic-Valentin et al.
2880 FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS
interaction witha GxDLS motif. Conversely, mutation
of the central Leu residue (Leu225 in HIC1), which is
the sole invariant residue in all CtBP-interacting motifs
known so far, also abolishestheinteraction between
HIC1 and CtBP1 or CtBP2. In close agreement with
the corepressor activity of CtBPs, this mutation
impairs the HIC1-mediated transcriptional repression.
These results thus demonstrate a strong conservation
in the recognition of the CtBP-interacting motifs
despite great divergence in their amino acid sequences.
Results
HIC1 can interact with both CtBP1 and CtBP2
in vivo
The vertebrate genomes contain two different genes,
CtBP1 and CtBP2, that encode two highly related
corepressors, CtBP1 and CtBP2, but with only parti-
ally redundant functions. It is thus important to deter-
mine if a given transcription factor can interact with
both corepressors. Theinteraction between HIC1 and
CtBP1 was first demonstrated through transient trans-
fection assays and ina stably tranfected HIC1 cell line
with inducible HIC1 expression [14]. We have recently
shown that endogenous HIC1 proteins can be detected
in ahuman medulloblastoma cell line, DAOY [15]. To
fully validate theinteraction between endogenous
HIC1 and CtBPs, we performed coimmunoprecipita-
tion experiments using DAOY total cell extracts and
either normal rabbit immunoglobulins or the anti-
HIC1 antibody. The immunoprecipitated proteins were
divided into two equal parts, separated by SDS ⁄ PAGE
and then immunoblotted with monoclonal antibodies
to CtBP1 or CtBP2. CtBP1 was detected withthe spe-
cific CtBP1 monoclonal antibodies inthe anti-HIC1
immunoprecipitates (Fig. 2A, top panel, lane 3) but
not in control IgG immunoprecipitates (lane 2). In
contrast, analyses of equal amounts from the same
immunoprecipitates withthe CtBP2 monoclonal anti-
bodies yield only nonspecific bands, presumably due
to the quality of the antibodies used (Fig. 2A, middle
panel, lanes 2 and 3). Coimmunoprecipitation of
DAOY nuclear extracts withthe monoclonal or
another commercial polyclonal CtBP2 antibody fol-
lowed by western blotting with an anti-HIC1 antibody
did not give better results, probably because CtBP2 is
associated with numerous partners (data not shown).
As control, HIC1 is detected inthe anti-HIC1 immu-
noprecipitates but not in control IgG immunoprecipi-
tates (Fig. 2A, bottom panel).
To confirm theinteraction observed between endog-
enous HIC1 and CtBP1 (Fig. 2A, top panel), we per-
formed another coimmunoprecipitation experiment
using a similar amount of DAOY total cell extracts
and another HIC1 antibody, the polyclonal 325 anti-
body directed against a C-terminal peptide of HIC1 or
normal rabbit immunoglobulins. The total immunopre-
cipitated proteins were separated by SDS ⁄ PAGE.
After transfer, the membrane was separated into two
parts. The upper part (proteins above 60 kDa) and the
lower part were directly immunoblotted, respectively,
with theHIC1 antibody and withthe CtBP1 monoclo-
nal antibody. CtBP1 was detected withthe specific
CtBP1 monoclonal antibodies inthe anti-HIC1 immu-
noprecipitates (Fig. 2B, lane 6) but not in control IgG
immunoprecipitates (lane 5). As a further control, pro-
teins immunoprecipitated from DAOY total extracts in
stringent conditions (RIPA buffer) with anti-CtBP1,
normal rabbit immunoglobulins or HIC1 polyclonal
antibodies were loaded on the same gel (Fig. 2B, lanes
1–3).
To determine if HIC1 can also interact with CtBP2
in vivo, we thus performed coimmunoprecipitation
assays in COS7 cells transiently transfected with
expression vectors encoding a FLAG version of human
HIC1 and CtBP1 or CtBP2, alone or in combination.
First, western blot analyses of total cell lysates with
monoclonal antibodies directed against CtBP1 or
CtBP2 demonstrated the specificity and the reactivity
of these antibodies (Fig. 2C, top and middle panels;
compare lanes 3 and 4). Immunoprecipitation of total
cell extracts withthe FLAG monoclonal antibody fol-
lowed by immunoblot withthe CtBP1 or CtBP2
monoclonal antibodies detected specific binding of
HIC1 not only to CtBP1 as previously described
(Fig. 2C, lane 11) but also to CtBP2 (Fig. 2C, lane 12)
in cotransfected cells.
CtBP has been proposed to be a redox sensor link-
ing gene expression and metabolism. Indeed, CtBP
binding to some, but not all, of its partners is potenti-
ated by hypoxia or treatment with agents such as
CoCl
2
, both of which increase the level of free NADH
[26,27]. We therefore investigated the effect of CoCl
2
treatment on theinteraction between HIC1 and
CtBP1. COS7 cells were transfected with expression
vectors for HIC1 and CtBP1 and treated with 200 lm
CoCl
2
for 3 h before lysis. After immunoprecipitation
with theHIC1 antibody, a significant increase in the
amount of CtBP1 coimmunoprecipitated was observed
in the presence of CoCl
2
(Fig. 2D; compare lanes 6
and 8), indicating that theinteraction between HIC1
and CtBP1 is favoured inthe presence of higher
NADH levels.
Thus, HIC1 can interact in vivo with both the CtBP1
and CtBP2 corepressors.
N. Stankovic-Valentin et al. HIC1 interacts with CtBP
FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS 2881
The integrity of the substrate-binding domain
of CtBP2 is required for recognition of
the GLDLSKK motif
Crystal structure analyses of the substrate-binding
domain of CtBP1 have highlighted crucial residues
involved intheinteractionwitha PIDLSKK-like
peptide. Notably, point mutations A41E or V55R,
involving two residues that directly contact the PID-
LSKK peptide, are sufficient to abolish the interaction
between the E1A C-terminus and CtBP1 [28].
HIC1 is the first transcriptional factor known to
recruit CtBP via a divergent GLDLSKK motif in
which the Pro, which was considered to be an invariant
A
IP HIC1 (2563)
Input 2%
IgG
WB: CtBP2
WB: CtBP1
CtBP1
*
1 2 3
HIC1
WB: HIC1
(325)
B
GgI
IH
)
5
2
3
( 1
C
%2
tu
p
nI
)523( 1CIH
)8
21
1( 1P
B
tC
G
gI
IP
(RIPA Buffer)
Co-IP
(IPH Buffer)
HIC1
CtBP1
WB: HIC1
(325)
WB: CtBP1
(E-12)
1 2 3
456
C
GALF
1CIH-GALF
1PBtC
2P
BtC
1PBtC+1CIH-GALF
2PBtC+1CIH-GALF
GALF
1CIH-GALF
1PBtC
2
P
Bt
C
1
PBtC+1CIH-GALF
2P
B
tC+1CIH-GALF
WB: CtBP1
WB: CtBP2
WB: HIC1 (325)
Input 5%
1 2 3 4 5 6
7 8 9 10 11 12
IP FLAG
D
+ CtBP1 + CtBP1
G
ALF
1
C
IH
-GA
L
F
GALF
1CIH-GALF
GAL
F
1CIH
-
GAL
F
GALF
1
CIH-G
A
LF
+CoCl
2
+CoCl
2
WB: CtBP1
WB: FLAG
1 2 3 4
5 6 7 8
Input 5% IP: HIC1(2563)
Fig. 2. In vivo, HIC1 can interact with C-terminal binding protein 1 (CtBP1) or CtBP2. (A) Endogenous HIC1 and CtBP1 interact. DAOY cells
were lysed with IPH buffer, and 2% of the lysates were kept as input (lane 1). Equal amounts of lysate were then immunoprecipitated with
normal rabbit immunoglobulins (lane 2: IgG) or the rabbit HIC1 antibodies (lane 3: HIC1 (2563)). The immunoprecipates were divided in two
and analysed in parallel by immunoblot with CtBP1 (top panel) or CtBP2 (middle panel) monoclonal antibodies. The anti-CtBP1 membrane
was stripped and analysed withtheHIC1 polyclonal antibodies as control (bottom panel). *Nonspecific band. (B) Endogenous HIC1 and
CtBP1 interact. DAOY cells were lysed with RIPA buffer in stringent conditions, and lysates were immunoprecipitated withthe rabbit CtBP
antibodies (lane 1: aCtBP1(1128)) [38], with normal rabbit immunoglobulins (lane 2: IgG) or another rabbit HIC1 antibody (lane 3: aHIC1
(325)). The same amount of DAOY cells as used in (A) were lysed with IPH buffer, and 2% of the lysates were kept as input (lane 4). Equal
amounts of lysate were then immunoprecipitated with normal rabbit immunoglobulins (lane 5: IgG) or the other rabbit HIC1 antibodies (lane
6: aHIC1 (325)). The immunoprecipates were loaded and analysed directly by immunoblot withthe polyclonal HIC1 325 (top panel) or the
monoclonal CtBP1 E-12 (bottom panel) antibodies. (C) In vivo, HIC1 interacts with either CtBP1 or CtBP2. COS7 cells were transfected with
the above indicated expression vectors. Five per cent of each lysate was directly resolved by SDS ⁄ PAGE and immunoblotted withthe indica-
ted antibodies (input 5%, lanes 1–6). Each cell lysate was immunoprecipitated withthe FLAG M2 antibody (IP FLAG, lanes 7–12). The immu-
noprecipitates were then analysed by western blotting witha CtBP1 monoclonal antibody (top panel). The membrane was then stripped and
reprobed witha CtBP2 polyclonal antibody (middle panel). Finally, the presence of HIC1inthe immunoprecipitates was confirmed by immu-
noblotting withtheHIC1 polyclonal antibody (lower panel). (D) Treatment with CoCl
2
increases theinteraction between HIC1 and CtBP1.
COS7 cells were transfected withthe indicated expression vectors. Forty-eight hours after transfection, cells were either treated with
200 l
M CoCl
2
or mock-treated for 3 h and lysed in IPH buffer. Immunoprecipitates were analysed by western blotting as described above
with CtBP1 or FLAG monoclonal antibodies.
HIC1 interacts withCtBP N. Stankovic-Valentin et al.
2882 FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS
residue, is replaced by a Gly [14]. The interaction
between HIC1 and CtBPs could thus involve residues
different from those involved intheinteraction via a
consensus PIDLSKK peptide. To address this question,
the equivalent point mutations were introduced by site-
directed mutagenesis into CtBP2 [29] to yield A58E
and V72R. Like wild-type (wt) CtBP2, these mutants
are able to dimerize normally (data not shown). We
first used the yeast two-hybrid assay to investigate the
interaction between the central region (CR) of HIC1
(amino acids 135–422) and wt or mutant CtBP2. Yeast
cotransformed withtheHIC1 CR fused to the Gal4
DNA-binding domain (DBD) and the CtBP2 point
mutants fused to the Gal4 activation domain (AD)
were unable to grow on His-selective medium (Fig. 3A,
right panel), similar to those transfected withthe empty
Gal4 vectors as negative control. As expected, the inter-
action between theHIC1 CR and wt CtBP2 restores
the growth on selective medium.
These results were confirmed inthe context of the
full-length proteins by coimmunoprecipitation analyses
after transient transfection in COS7 cells. In this assay,
a strong interaction is observed between wt HIC1 and
CtBP2 (Fig. 3B, lane 12), whereas the A58E and V72R
point mutants fail to interact withHIC1 (Fig. 3B,
lanes 14 and 16).
Thus, binding of a CtBP-interacting partner contain-
ing a PxDLS or a GxDLS motif is mediated by the
same peptide recognition cleft intheCtBP N-terminal
region.
Point mutation of the only invariant residue
in the CtBP-interacting domain is sufficient to
abolish the HIC1–CtBP interaction
Having established that theinteraction between the
HIC1 GxDLS motif and CtBP1 or CtBP2 occurs by
binding to the same peptide cleft that binds typical
A
Gal4DBD
Gal4DBD
HIC1 CR
SD-Leu -Trp SD-Leu -Trp -His
Gal4AD
Gal4AD
CtBP2
Gal4AD
CtBP2 A58E
Gal4AD
CtBP2 V72R
Gal4DBD
Gal4DBD
HIC1 CR
Gal4AD
Gal4AD
CtBP2
Gal4AD
CtBP2 A58E
Gal4AD
CtBP2 V72R
B
GALF
1CIH-GALF
2PBtC
2PBtC + 1CIH-G
A
L
F
E85A 2PB
t
C
E85A 2PBtC + 1CIH-GALF
R27V 2PBtC
R
27V
2
PBt
C
+
1CIH-GALF
GALF
1
C
IH-G
ALF
2
P
BtC
2PBtC +
1
C
IH-GALF
E8
5
A 2PB
t
C
E8
5
A 2PBt
C
+ 1CI
H-
G
A
L
F
R
27
V 2
P
B
t
C
R
27V
2
PB
tC
+
1
C
I
H
-GALF
*
1 2 3 4 5 6 7 8
9 10 11 12 13 14 15 16
WB:CtBP2
WB: HIC1
Input 5% IP HIC1 (2563)
Fig. 3. The GLDLSKK motif of HIC1 con-
tacts the same residues in CtBP2 as the
consensus PIDLSKK motif. (A) In yeast two-
hybrid assays, CtBP2 A58E and CtBP2
V72R fail to interact withthe central region
of HIC1. The yeast two-hybrid assay was
used to assess theinteraction between the
central region of HIC1 containing the
GLDLSKK motif and murine CtBP2 mutants
A58E and V72R. Yeast cotransformed with
the plasmids shown grew on SD-Leu-Trp
media (left panel). These transformants
were patched onto SD-Leu-Trp-His selective
media (right panel). Growth after 48 h at
30 °C is shown. (B) Effect of the CtBP2
mutants A58E or V72R on HIC1–CtBP2 inte-
raction in vivo. COS7 cells were transfected
with the indicated expression vectors. Forty-
eight hours after transfection, cells were
directly lysed in IPH buffer. Five per cent of
each lysate was directly resolved by SDS ⁄
PAGE and immunoblotted withthe indicated
antibodies to control for HIC1 and CtBP2
protein expression (input 5%, lanes 1–8).
Lysates were immunoprecipitated with anti-
HIC1 (lanes 9–16) and analysed by western
blotting with anti-CtBP2 (upper panel) and
with HIC1 polyclonal antibody (lower panel).
*Nonspecific band detected by anti-CtBP2
in each extract under the conditions used.
N. Stankovic-Valentin et al. HIC1 interacts with CtBP
FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS 2883
PxDLS-containing partners, we next investigated the
role of the central Leu intheHIC1 GxDLS motif,
which is now the sole invariant residue inthe CID.
This Leu was thus mutated to Ala (mutation L225A)
to yield a GxDAS motif (Fig. 4A). We first conducted
mammalian two-hybrid assays in rabbit kidney cells
(RK13). Chimeras between the Gal4 DBD and a trun-
cated CR of HIC1 (amino acids 135–296) [14] contain-
ing or not containing theL225A point mutation were
tested for their ability to interact with full-length
CtBP1 fused to the VP16 AD. A chimera between the
Gal4 DBD and theHIC1 BTB ⁄ POZ domain was used
as a negative control (Fig. 4A, lane 2). As previously
described [14], this truncated CR of HIC1 strongly
interacts with CtBP1 (Fig. 4A, lane 3), whereas the
point mutation of the central Leu to Ala abolished this
interaction (Fig. 4A, lane 4).
These results were obtained using the isolated CR of
HIC1. To confirm the essential role of the central
Leu225 inthe context of the full-length protein, we
next performed coimmunoprecipitation experiments.
As shown above, the full-length HIC1 protein can
interact with CtBP1 (Fig. 4B, lane 4) or CtBP2
(Fig. 4C, lane 5), whereas theL225A point mutant is
unable to interact with CtBP1 (Fig. 4B, lane 6) or
CtBP2 (Fig. 4C, lane 6).
In conclusion, mutation of Leu225 of the
GLD
LSKK motif intheHIC1 CID domain into an
Ala (GLDASKK) is sufficient to abolish the interac-
tion between HIC1 and CtBP1 or CtBP2 in vivo.
The HIC1 central region functions as a
CtBP-dependent and CtBP-independent
autonomous repression domain
We next investigated the importance of this L225A
point mutation for the transcriptional repression prop-
erties of the whole CR of HIC1. To that end, the
entire domain (amino acids 135–422) located between
the BTB ⁄ POZ domain and the first zinc finger motif
was cloned in frame withthe Gal4 DBD and tested in
the Gal4 repression assay. Upon transient transfection
into RK13 cells, Gal4-HIC1 (135–422) efficiently
repressed the expression of a reporter gene containing
five Gal4-binding sites (Fig. 5A, lane 2). Notably, the
L225A mutation, which abolishestheinteraction with
CtBP1 and CtBP2 (Fig. 4B,C), significantly reduced
but did not totally abolish the repression potential of
the CR (Fig. 5A, lane 3).
CtBP is found ina large multiprotein complex con-
taining HDACs [30]. When these experiments were
performed inthe presence of TSA, a specific inhibitor
of class I and class II HDACs, the repression exhibited
both by the wt and theL225A chimeras was signifi-
cantly reduced (Fig. 5A).
A similar effect was observed, albeit to a lesser
extent, when the repression of the wt and L225A full-
length HIC1 proteins were tested on a Luc reporter
gene driven by the recently defined HIC1-responsive
element, 5xHiRE [15] (Fig. 5B). This relatively mild
effect on repression is probably explained by the pres-
ence of the BTB ⁄ POZ domain, which is another potent
autonomous repression domain.
Thus, theHIC1 CR represses transcription through
CtBP-dependent and CtBP-independent mechanisms
and involves the recruitment of class I or class II
HDACs.
Discussion
In this report, we demonstrate that thetumour sup-
pressor gene and transcriptional repressor HIC1 inter-
acts withthe related but not fully functionally
redundant CtBP1 and CtBP2 corepressors in vivo and
that this interaction is regulated by CoCl
2
, hence
connecting the HIC1-mediated repression to NAD
+
⁄
NADH levels and hypoxia. Moreover, mutation of the
invariant Leu residue, L225A, is sufficient to disrupt
the interaction between HIC1 and CtBPs.
CtBPs have been previously shown to bind to repres-
sion domains ina number of transcription factors and
other regulatory proteins. Inthe vast majority of CtBP
partner proteins, a PxDLS motif has been shown to be
the primary determinant of CtBP binding, which has
led to the hypothesis that the PxDLS motif slots into
an ‘accepting’ pocket in CtBP. However, some variant
motifs have been reported, such as a bipartite motif
located inthe C-terminus of the viral EBNA3A pro-
tein, where two nonconsensus sites, ALDLS and
VLDLS, synergize to produce very efficient binding to
CtBP [31]. Other variants include a GLDLS motif in
HIC1 [14] and a GLELE motif in ArpNa, a brain-spe-
cific actin-related protein found in many chromatin-
remodelling and histone acetyltransferase complexes
[32]. Using theHIC1 GxDLS motif as a paradigm for
these nonconsensus motifs, we investigated the proper-
ties of its binding to CtBPs. In their elegant work on
the crystal structure of rat CtBP ⁄ BARS ina ternary
complex with NADH and a PIDLSKK peptide, Nard-
ini et al. [28] identified the peptide-binding site as a
hydrophobic surface cleft inthe N-terminal part of the
substrate-binding domain. As this cleft is lined with
hydrophobic residues that are fully conserved between
CtBP1 and CtBP2, two different point mutants were
constructed inthe PIDLS-accepting pocket of CtBP2.
In directed yeast two-hybrid assays or in transient
HIC1 interacts withCtBP N. Stankovic-Valentin et al.
2884 FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS
tranfection assays in mammalian cells, these two point
mutants are unable to interact withHIC1 (Fig. 3).
These results indicate that this cleft can accommodate
peptides with some variability in their amino acid
sequences and could be considered to be a general
binding site for (P ⁄ G ⁄ V ⁄ A)xDLS-containing partners.
A
VP16
VP16-CtBP1
1
2
3
4
296
296
DBD-Gal4
1
Fold activation
135
GLD
LSKK
135
GLD
ASKK
1
140
POZ
2
3
4
5 x gal4
Luc
WB: CtBP1
WB: FLAG
II
I
PIPI
PI
GALF
1CIH-GALF
1CIH-
G
ALF
A522L
B
+ CtBP1
IP HIC1(2563)
1 2 3 4 5 6
GALF
1CIH-GALF
1CIH-GALF
A
5
2
2L
+ CtBP1
CtBP2
FLAG-HIC1
FLAG-HIC1 L225A
IP: FLAG
WB: CtBP2
WB:HIC1 (325)
WB: CtBP2
WB: HIC1 (325)
Input 5%
12345 6
C
78 9 101112
WB: CtBP1
Input 5%
WB: FLAG
7 8 9
Fig. 4. Mutation of the central invariant Leu225 to Ala inthe GLDLSKK motif abolishestheinteraction between HIC1 and C-terminal binding
protein 1 (CtBP1). (A) In mammalian two-hybrid assays, HIC1 mutant L225A does not interact with CtBP1. Left panel: schematic structures
of the Gal4 DNA-binding domain (construct 1) and of the various Gal4-HIC1 chimeras (constructs 2–4). Numbering refers to HIC1 residues.
Black box, GLDLSKK motif; hatched box, mutated GLDASKK motif. Right panel: Luc and b-galactosidase assays were performed on total
extracts from RK13 cells that had been transiently transfected with 250 ng of the pG5-luc reporter (schematically drawn), 50 ng of the
pSG5-lacZ construct as a control of transfection efficiency, 50 ng of the indicated Gal4 construct and 150 ng of the VP16 activation domain
(grey bars) or VP16 activation domain-tagged murine CtBP1 (black bars). After normalization to b-galactosidase activity, the data were
expressed as Luc activity relative to the activity of the pG5-luc with empty control vectors, which was given an arbitrary value of 1. Results
presented are the mean values and standard deviations from two independent transfections in triplicate. (B) HIC1L225A mutation disrupts
its interactionwith murine CtBP1. COS7 cells were transfected with expression vectors for CtBP1 and the indicated FLAG construct. Forty-
eight hours after transfection, lysates were split into two and immunoprecipitated with rabbit preimmune serum (PI: lanes 1, 3 and 5) or the
rabbit HIC1 2563 polyclonal antibody (I: lanes 2, 4 and 6). The resulting immunoprecipitates were analysed by western blotting withthe indi-
cated monoclonal antibodies (anti-CtBP1, upper panel; and anti-FLAG M2, lower panel). Five per cent of each total cell extract (input) was
similarly analysed with CtBP1 and FLAG M2 antibodies to control for CtBP1 and HIC1 expression. *Nonspecific band. (C) HIC1L225A muta-
tion disrupts itsinteractionwith CtBP2. COS7 cells were transfected with expression vectors for CtBP2, FLAG-HIC1 and FLAG-HIC1 L225A
alone or in combination as indicated and the indicated FLAG. Input (lanes 7–12) and immunoprecipitates (lanes 1–6) were analysed as des-
cribed above withthe CtBP2 or HIC1 (325) antibodies.
N. Stankovic-Valentin et al. HIC1 interacts with CtBP
FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS 2885
Mutagenesis of the first Pro, of the Pro-Leu or of
the Asp-Leu residues inthe PLDLS CtBP-binding
motif from various proteins has been widely used to
severely impair their interactionwith CtBP, at least in
some assays. Based on the cocrystal structure [28],
binding of a PLDLS motif can be driven by docking
of the two Leu side chains into the conserved hydro-
phobic surface groove. However, to the best of our
knowledge, our work provides the first demonstration
that a unique point mutation of the central Leu resi-
due, which is the sole invariant residue in CID motifs,
is sufficient to ablate theinteraction between a
transcription factor and CtBPs. Our results are thus
not only in perfect agreement withthe structural data
but also provide experimental clues to the universal
conservation of this Leu residue in all CtBP-binding
motifs described so far.
The HIC1 CR appears to be a second repression
domain exhibiting both CtBP-dependent and CtBP-
independent repression mechanisms, both of which are
sensitive to TSA. Indeed, theL225A point mutation
(Fig. 5A) as well as the deletion of the GLDLS motif
[14] impaired but did not fully abolish the transcrip-
tional repression mechanisms of the CR. The other
corepressors and complexes interacting withthe HIC1
CR are currently being investigated. Along these lines,
we have recently identified a SUMOylation site at
Lys314 that plays a role inthe repression potential of
this region (Stankovic-Valentin et al., unpublished
results). In addition, a yeast two-hybrid screen per-
formed withtheHIC1 CR as bait identified not only
full-length CtBP1 and CtBP2 as HIC1 partners
but also some proteins clearly associated with HDAC-
containing complexes (C. Fleuriel and D. Leprince,
unpublished results).
Finally, we are currently generating conditional
mouse knock-in (KI) mutants harbouring the critical
Leu-to-Ala substitution at position 225, inthe CtBP-
interacting domain of HIC1. These animal models
could be very useful for directly addressing in vivo the
role played by the HIC1–CtBP interactionin two
mutually nonexclusive pathways: theHIC1 tumour
suppressor properties, which are altered in many
human cancers, and normal development.
CtBP appears to be a potential modulator of apop-
tosis and epithelial-to-mesenchymal transition (EMT),
an important feature of embryonic development and
tumorigenesis [33]. The heterozygous Hic1
+ ⁄ –
mice are
viable, and have no obvious developmental defects,
but spontaneously develop tumours late in their life
with a predominance of sarcomas and lymphomas in
females and of carcinomas in males [2]. It would thus
be interesting to determine if heterozygous mice carry-
ing theL225A point mutation inthe CtBP-binding
domain, Hic1
+
⁄ KI, will also develop tumours, especi-
ally the males.
More importantly, the homozygous Hic1
– ⁄ –
mice are
embryonic lethal and display severe developmental
anomalies, some of which are found in MDS patients
[9]. In Drosophila, CtBP is acorepressor for several
short-range repressors essential for early embryonic
development, such as Kru
¨
ppel, Giant, Knirps and Snail
[34]. In vertebrates, Ctbp1-orCtbp2-deficient embryos
exhibit a large variety of developmental defects, consis-
tent withthe fact that they interact witha multitude of
A
RK13
5 x gal4
Luc
DBD-Gal4
1
GLDLSKK
422
135
2
GLDASKK
422
135
1
2
3
4
5
6
7
DMSO
TSA
3
Fold repression
B
U2OS
5 x HiRE
Luc
1234
FLAG
FLAG-HIC1
FLAG-HIC1 L225A
Fold repression
Fig. 5. Mutation L225Ainthe CtBP-binding motif impaired the
HIC1-mediated transcriptional repression. (A) TheHIC1 central
region contains C-terminal binding protein (CtBP)-dependent and
CtBP-independent repression activities which are both trichostatin
A (TSA)-sensitive. Left panel: schematic structure of the Gal4 DNA-
binding domain and the two Gal4-HIC1 chimeras. Numbering refers
to HIC1 residues. Black box, GLD
LSKK motif; hatched box,
mutated GLD
ASKK motif. Right panel: RK13 cells were transiently
transfected in triplicate with 100 ng of the indicated constructs,
350 ng of the pG5-Luc reporter and 50 ng of the pSG5-LacZ vector.
The cells were treated 24 h later with 300 n
M TSA (dissolved in
dimethyl sulfoxide (DMSO)) (white boxes) or mock-treated with an
equal volume of DMSO (black boxes) for a further 24 h before har-
vesting. Luc and b-galactosidase assays were conducted as des-
cribed in Fig. 4A. (B) TheL225A mutation affects the transcriptional
repression potential of full-length HIC1. U2OS cells were transfect-
ed with 225 ng of the indicated expression vector, 225 ng of the
reporter 5xHiRE-luc plasmid and 50 ng of the pSG5-lacZ construct
as control of transfection efficiency. Luc and b-galactosidase assays
were done as described above.
HIC1 interacts withCtBP N. Stankovic-Valentin et al.
2886 FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS
transcription factors involved in many signalling path-
ways [21]. For all these reasons, it is tempting to specu-
late that theHic1 KI ⁄ KI embryos could phenocopy, at
least in part, the abnormalities found inthe Hic1
– ⁄ –
mice. For example, GATA2 KI ⁄ KI mice carrying a
Val-to-Gly point mutation inthe GATA2 N-terminal
zinc finger that ablates theinteractionwith cofactors of
the Friend of GATA (FOG) family have been gener-
ated. These mutant mice display complete megakaryo-
poietic failure, a phenocopy of Fog1
– ⁄ –
mice [35].
Focusing on CtBP, the best example in favour of
our working hypothesis is holoprosencephaly (HPE),
the most common structural defect of the developing
forebrain in humans, frequently accompanied by cra-
niofacial anomalies. HPE4, one of the loci associated
with this disease, maps to TGIF, a gene encoding a
homeodomain transcriptional repressor modulating
NODAL (a member of the transforming growth
factor-b family) signalling. Individuals with HPE carry
heterozygous mutations either inthe DNA-binding
domain, the SMAD-binding domain, or a CtBP-inter-
action motif [36]. This latter S28C mutation converting
a canonical PLDLS motif into a PLDLC motif is suffi-
cient to disrupt theinteraction between TGIF and
CtBP [37], as does theL225Ain HIC1.
In summary, we have shown that HIC1 is able to
interact with CtBP1 and CtBP2. We have demonstra-
ted that point mutation of the central Leu225 in the
CtBP-interacting motif is sufficient to abolish the inter-
action with CtBP, which is in perfect agreement with
the structural data and the universal conservation of
this residue in all CID motifs. This point mutation
could also provide useful KI animal models to study
the role of the HIC1–CtBP interactionin tumorigen-
esis as well as in development.
Experimental procedures
Constructs
The full-length FLAG-HIC1 L225A point mutant was gen-
erated by the two-round PCR mutagenesis strategy using
the following two mutagenic oligonucleotides, which
introduced an Nsi1 restriction site (underlined) (5¢-CGG
CGGGCTCTTCTTGG
ATGCATCCAGGCC-3¢ and 5¢-GG
CCTGG
ATGCATCCAAGAAGAGCCCGCCG-3¢) and
convenient flanking oligonucleotides. The StuI–XhoI frag-
ment containing theL225A mutation was exchanged with
the same restriction fragment inthe previously described
FLAG-HIC1 wt expression vector [14].
The Gal4-HIC1 135–296 wt and L225A were generated
by PCR using the wt or L225A full-length clones as matri-
ces and the following oligonucleotides: sense 5¢-GGAATT
CG
GGATCCCAAAGTACTGCCACCTGCGG-3¢ with a
BamH1 site, and antisense 5¢-AGT
GGTACCGTCGACTC
ATCCCGGGCTGCCGCT-3¢, witha KpnI site. The Gal4-
HIC1 135–422 wt and L225A were generated by PCR as
described above withthe same sense oligonucleotide and
an antisense oligonucleotide containing an SstI site 5¢-AT
GCACACACGTAAGGCACTCAGCTGAGAT
CTCGAG-
3¢. The BamHI–KpnI and BamHI–SstI restriction fragments
were cloned in frame withthe Gal4 DBD inthe pSG5424
vector. A58E and V72R mutations were introduced into
mCtBP2 by overlap PCR mutagenesis (mCtBP2.A58E.F,
GACCTGGCCACTGTGGAATTCTGTGATGCACAG;
mCtBP2.A58E.R, CTGTGCATCACAGAATTCCACAGT
GGCCAGGTC; mCtBP2.V72R.F, GAAATCCATGAGA
AGCGGTTGAATGAAGCTGTG; and mCtBP2.V72R.R,
CACAGCTTCAT TCAACCGCTTCTCATG GATTTC).
BglII- and SalI-digested mutant inserts were ligated into
the BamHI–SalI sites of pGAD10 vector to generate
Gal4AD-CtBP2-A58E and Gal4AD-CtBP2-V72R. Second,
wt CtBP2, CtBP2-A58E and CtBP2-V72R mutant inserts
were reamplified by PCR using appropriate primers and
cloned into the NotI–SalI sites of pMT3 (derived from
pMT2).
PCR fragments were systematically verified by sequen-
cing on both strands. All clones were checked by appropri-
ate restriction enzyme digestion, and the vector–insert
junctions were verified by sequencing.
Yeast two-hybrid system
The yeast two-hybrid system was used as described in the
manufacturer’s protocol (Clontech, Saint Quentin en Yve-
lines, France). Briefly, the CR of HIC1 [14] was cloned in-
frame into the Gal4 DBD plasmid pGBT9. These plasmids
were cotransfected withthe Gal4 AD plasmid pGAD10
fused to mCtBP2, A58E and V72R mutants into the yeast
strain HF7c, and transformants were selected on Trp ⁄ Leu-
deficient media (SD-Leu-Trp). Colonies were patched onto
Trp ⁄ Leu ⁄ His-deficient media (SD-Leu-Trp-His).
Cell culture and transfection
COS7, DAOY, U2OS and RK13 cells were maintained in
Dulbecco medium supplemented with 10% fetal bovine
serum.
Cells were transfected in OptiMEM (Gibco, Paisley, UK)
by the PEI (Euromedex, Souffelweyersheim, France) method
as previously described [14] in either 100 mm diameter
dishes (in vivo interactionin COS7 cells) with 2.5 lgof
DNA or in 12-well plates (repression and mammalian two-
hybrid assays) with 500 ng of DNA for RK13 cells and for
U2OS cells. Cells were transfected for 6 h and were then
incubated in fresh complete medium. They were rinsed in
cold NaCl ⁄ P
i
48 h after transfection and processed for
N. Stankovic-Valentin et al. HIC1 interacts with CtBP
FEBS Journal 273 (2006) 2879–2890 ª 2006 The Authors Journal compilation ª 2006 FEBS 2887
coimmunoprecipitation, repression or mammalian two-
hybrid assays as described below.
Immunoprecipitation and coimmunoprecipitation
Total DAOY cell extracts were immunoprecipitated in
stringent conditions using RIPA buffer as previously des-
cribed [14].
For coimmunoprecipitation experiments, DAOY cells or
COS7 cells (48 h after transfection) were rinsed twice in
cold NaCl ⁄ P
i
and lysed in cold IPH buffer (50 mm Tris
pH 8, 150 mm NaCl, 5 mm EDTA, 0.5% NP-40, protease
inhibitor cocktail (Roche)). Cell lysates were cleared by cen-
trifugation (20 000 g, 30 min). The supernatants were incu-
bated overnight with 4 lL of antibody. Then, protein A
Sepharose beads (Amersham Biosciences, Orsay, France)
were added for 1 h. The beads were washed three times
with IPH buffer. Proteins were eluted by boiling in Lae-
mmli loading buffer and separated by SDS ⁄ PAGE before
western blotting. For experiments using CoCl
2
, 3 h before
lysis, cells were either treated with 200 lm CoCl
2
diluted in
fresh medium or mock-treated with fresh medium.
Repression and mammalian two-hybrid assays
Forty-eight hours after transfection, cells were rinsed in
NaCl ⁄ P
i
and lysed withthe Luc assay buffer (25 mm glycyl-
glycine, pH 7.8; 15 mm MgSO
4
;4mm EGTA; 1% Triton
X-100). Luciferase and b-galactosidase activities were meas-
ured by using, respectively, beetle luciferin (Promega, Char-
bonnieres, France) and the Galacto-light kit (Tropix,
Bedford, MA, USA) witha Berthold (Thoiry, France)
chemioluminometer. After normalization to b-galactosidase
activity, the data were expressed as Luc activity relative to
the activity of pG5-Luc with empty control vector, which
was given an arbitrary value of 1. Results represent the
mean values and standard deviations from two independent
transfections in triplicate [12].
Western blot and antibodies
Western blots were performed essentially as previously des-
cribed [14]. TheHIC1 2563 and HIC1 325 antibodies have
been previously described [14]. For CtBP1, the CtBP1
monoclonal antibody (E-12) raised against amino acids 1–
440 of human CtBP1 (Santa Cruz Biotechnology, Le perray
en Yvelines, France) was used. To detect endogenous
CtBP2, we used two different antibodies: a monoclonal
antibody raised against amino acids 361–445 of mouse
CtBP2 (BD Biosciences, Pharmingen, Le Pont de Claix,
France) or a goat polyclonal antibody raised against a pep-
tide near the C-terminus of CtBP2 of human origin
(sc-5966; Santa Cruz Biotechnology). Inthe immunoprecip-
itation experiment, we used the CtBP1 rabbit polyclonal
1128 antibodies raised against the ovalbumin-coupled pep-
tide corresponding to amino acids 352–374 of murine
CtBP1 [38] and kindly provided by B. Wasylyk. Anti-Flag
M2 is a monoclonal antibody (F3165; Sigma, Lyon,
France). The secondary antibodies were horseradish peroxi-
dase-linked antibodies raised against either rabbit or mouse
immunoglobulins (Amersham Biosciences).
Acknowledgements
We thank Drs Se
´
bastien Pinte and Brian R. Rood for
critically reading the manuscript. This work was sup-
ported by funds from CNRS, the Pasteur Institute, the
Ligue Nationale contre le Cancer (Comite
´
Interre
´
gio-
nal du Septentrion), the Association pour la Recherche
contre le Cancer (ARC) and a grant from the Austra-
lian National Health and Medical Research Council.
N. Stankovic-Valentin was supported by a fellowship
from the Ministe
`
re de la Recherche et de la Technolo-
gie and by the Association pour la Recherche contre le
Cancer (ARC).
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Gal4AD
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Gal4DBD
Gal4DBD
HIC1 CR
Gal4AD
Gal4AD
CtBP2
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CtBP2 A5 8E
Gal4AD
CtBP2 V72R
B
GALF
1CIH-GALF
2PBtC
2PBtC. mutation of the only invariant residue
in the CtBP- interacting domain is sufficient to
abolish the HIC1 CtBP interaction
Having established that the interaction