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I ran Thj Pha va Dig Tap ehi KHOA HOC & CONG NGHE 90(02) 101 105 N ( ; H I E N C U U ANH HUONG CUA pH DEN KHA NANG HAP THU ASEN (As) V \ CHi (Pb) CUA C AY SAY (Phragmiles auslralis) Tran Thj Pha''''"'''' Ba[.]
I r a n Thj Pha va Dig 90(02): 101-105 Tap ehi KHOA HOC & CONG NGHE ANH HUONG CUA pH DEN KHA NANG HAP THU ASEN (As) V.\ CHi (Pb) CUA C AY SAY (Phragmiles auslralis) N(;HIEN CUU Tran Thj Pha'"' Bang Van M i n h ' , Le Buc", Bam Xuan Van' ' Tnrirng Dut hgc \ dng Idm DH Thdi Sguren Tnrirng Dut hoc Khoa hoc tir nhii'n - DH Qudc gia Hd ,\ in 1(')M T A I Nghien ciru anh hudng ciia pH den kha nang hap thu Asen va Chi ciia cay siiy cho thay ham lugng As va Pb deu dugc cay sav lich liiy than la vai cac mirc dp khac tiiy thudc vao pH, •frong dd khii niing lich liiy As va Pb than * la vii re ciia cav siiy lot nhat o C l ' l la 20.12 ppm, 75.90ppm ddi vcri As vii 25.67ppm, 94.3 ppm ddi vcri Pb Kha niing xir ly dat nhiem As giam 1112,10 lan den 9.s9 liin vii diil nhiem chi giam tir ^.64 lan den 12.32 lan so voi ban diiu Tu' khoa: car sdy d nhiem ddl kha ndng lich liiy, pH DAT V A N DL Niii d u n g nghien cii'u Viin dc d nhiem k i m loiii nang diit cang dang quan tiim anh hudng true tiep den sirc khde ngudi v i cay trdng, Kha niing lam saeh mdi tnrdng cua thuc vat da diriTc ghi ckcp tir tke k\ X V l l l nhung den cudi the ky X X plurang pluip mdi dugc nhiic den nlur mdt cdng nghe tiin tien dimg de xtr ly mdi trtrdng dat va mrdc bi d nkiem bdi cac kim loiii nang ( K L N ) O lurdc la diiy la mdl phuong pluip x u ly liit mdi me va ckua dugc quan tiim nhicu Danh gia ank kirdng cua pi I den kkii nang sink tnrdng kap tku As vii Pb cua ciiy siiy P h u o n g phap nghien c i i u Phirang phdp xdy dung dudng chudn Xay dirng dudng cliiuin pH theo phuong phap Jensen Phinrng phdp ke thira Thu tkiip tlii lieu, sd lieu ve tink kinh dat bi d nhiem K I N tai Thai Nguyen Phmmg phdp hd tri thi nghiem Sav la loiii ca\ cd tke sdng ti'ong nkirng dieu T k i nghiem dugc dat nha ludi mdi ekau kicMi thdi tiet khiic nghicl va rat plui hgp vdi kg dat gdm tki ngkiem vdi cdng tkirc hiiu \ ' i c i N a m He sink viit quanh re Ipai mdi cdng thirc nhac lai lan tdng sd ekau tki cay luiv cd tke luip thu k i m loai nang va pk.in ngkiem: thi nghiem x x = 18 ekau liny cluil hiru co Thi nghiim NOI C k o vao m d i t r u d n g diit m u d i N a i H A s O i DLNG V.A PHI ONG PHAP H : v d i n d n g la I p p n i c i m g v d i As N G I I H N CU'U cd t r o n g diit la p p m Vay t h i n g k i e m Ddi tu-irng nghien c i i u Ddi lugng nghien ciru ki ciiy Say auslralis) I n d n g As t r o n g diit (Phragmiles ki mpl loai cay ldn thudc hg Hda la 143.85ppin dugc bd t r i d c d n g tkirc kin nkiic l a i \'di CTI c k o vao dat 0(g) CaCO-,, C I thao (A)i;c'ei;e) cd ngudn gdc o nhiing vting cko Viio diit 3(g) C a C O ; diit lay o ca kku viic nkiel ddi va dn ddi tren 54.6(g) CaCO-, t u o n g irng v d i cac muc pFI tke gioi can lam t k i n g k i e m K L N ngh ien ciru ki As va Pb dirtic bd sung Thi nghiim v i o diil diroi diing mudi d mdi Irudng dat cd Cho vao mdi trudng diil mudi P b ( N O : \ d i C'T3 cko vao dat ndng dd la OOOppm cimg vdi Pb cd dat pi I khiic nkau la I6.49ppin T k i ngkiem 2, ndng dp Pb trpng /(•/ "'ts2 irrliiia I.mail phucam20(i4 a rahoo com dat la I016.49ppin dugc bd tri a cdng ikirc 101 Tran Thj Pha vo D;a Tap chi KHOA HOC & CONG NGHE 90(02): 101 - 105 (CT) lan nhac lai \'di CTI cho vao dat 0(g) CaCO., CT2 cho vao dat 3(g) CaCO- CT3 cho vao dat 52.6(gi CaCO- tirang irng vdl cac mirc pH can lam thi nghieni Cho tkem Cho tkem Phirang phdp phdn nghiem Cho vao binh sd ": 20ml Ca(OH): 0,()5N tkem nude cat cho du 50nil cd pH = ''.86 Lac deu 30 phut rdi de yen ngiiy kic qua giay Igc pH bang may tich phong thi Phdn tich cdc chi lieu irong ddt PHK( i; Dugc chiet bang KCI IN bang may pll meter Mun (\K) Phan tich bang phucmg phap tiu rin Dam long sd iNi Phan tich bang phuang pkap Kieldakl I vao binh sd 10ml Ca(OH): O.OfN nudc cat cho du 50ITII cd pH = ~.51 vao binh so 6: 15ml Ca(OH); 0.05\ nude cat cho du 50ml co pH = 1."' ~_ "' > = 0567 - a362 R^ = 9133 ' 1 Lan tdng sd (P:0«): Phan tick bang phirong phiip so mau "ing lich trao ddi cation (mgdl lOOg dat) c1 C Phiin tick bang phuang phap \nioniaxetat As va Pb dat vii cay dugc xac dinh bang biing may ASS M6 - Ihermo Phinrng phdp xir ly so lien So lieu dugc tdng hgp phan tich va xtr ly biing pluin mem Misrosoli I xcci va SAS Ciic chl ticu theo ddi - Chl lieu ve khii nang sinh tnrong ctia diy thi nghiem: chieu cao cay sd diy chieu dai la sd la KFT(,)1 \ N ( , H U N C H \',\ IIIAOLLAN Xay dung dirdng chuiin pH X.iv dung dudng ckuan pH tkeo phuong pkiip Iciiscn, Lay " binh tam giac 100ml cho vao mdi binh lOg dat khd da ray qua riiv cd dirdng kinh I mm danh dau tir I den Lan lugt cho vao binh tam giac ndi Ircn mdt lutrng Ca(OH): iidng dd 0.fi5N nhu sau; Cho vao binh sd I: 2ml Ca(OH): 0.05N them ntrdc cat cho du 50ml co pH = 6.8 C ho vao binh sd 4nil Ca(OH); 0.05N them mioc cit cho du 5iinil co pH = ~.0 C~ho v,io binh s6 3" 6ml CaiOH); 0.05N them nuoc cat cho du ^Oml ^,i pH = ".24 Clio vao binh sd m l C a ( l l i 0.05N them niroc cil cho du 50ml co pH = 7.43 1112 Hinh I Bien ihli'n dm'mg chudnpH Tir kinh ta cd ducmg chuan xiiy dung moi tirang quan y = ax ^ b y: la mat dp pH dugc x: la ham lucmg Ca" mau Qua thi tren ta tkay he sd tuang quan r d mirc qiiaii he x va v troiu: quan he tuyen tinh r = \R' = sO.Q13.3= 0.956 Vdi gia tri RO.OKr = 0.956 nhu vay tucmg quan dudng chuan pH la tucmg quan tuyen tinh manh ado tin Ciiv la 99% Danh gla anh hu-ong cua cac pH khac den kha nang sinh tru'dng ciia cay Siiv moi tryd'ng dat cd chira KLN Dgc tinh hda ly vd hdm Iinrng kim logi ngitj; ddt trudc Irdng edy Dac tinh mdi trudng dat la: Dat chua va ngheo dinh dudmg bai vi dat cd piki 4.8 kam lugng mun la ( A l 1.03% kali long sd K:0: 0.49' o v a lan tdng so P;0= 0.06% dam tdng sd N i).!)"'"', dung tick trao diii cation CEC; 15.25mgdl lOOgam dat; Dat khdng d nhiem As va Pb vdi ham lugng toni: s6 As 3.85 ppm; Pb: 16.49 ppm (thap hon IC \ N lin lugt la 3.12 lin v a 4.24 lin) Thiti gian thi nghiem la thang (tir 05 01 05 05 2011) Ciic mudi gom: NaT! \sO 'H.iJva PbiNO) Trin Thj Pha vd Dig Tap chi KHOA HOC & CONG NGHE 90(02): 101 - 105 Bang Ddc linh ly- hoa' vd hdm lircrng kim logi ndng Irong ddl triri'rc irdng cdy Chi lieu pfhci Ham lugng 4.8 o\i ,V (%) 0,07 1.03 P.-O, (%) 0.06 Zn,, (ppm) 77,25 Cd„ (ppm) 1,89 CECdngdUinOgamr dat) 15,25 K;0 Co) 0.49 Ddnh gid dnh hudng cda cdc pH khdc den kha ndng sinh trirdng ciia cdy Sdy mdi truirng ddt ed chira As vd Ph Ket qua xiic dinh kha nang sinh trudng ciia cay Sav dudi anh hirdng cua cac pH cimg mdt ndng dp As va Pb dat duge trink bay d bang Bang Anh hirong ctia pH den kha ndng sinh trirong cua cin- Sdv mdi trirdng ddt cd chiru A s vd Ph Thi nghiem Thi nghiem (As) Thi nghiem (Pb) CT So cay Chieu cao cav (cm) Chieu diii lii (cm) CTI 6,1 ±0,26" 36.9 I 0.14' 33.64 ± 0.55' cr2 5.2 t 0,1'' 34.13 - 0.81'' 31.67±0.42'' 4.7 ±0,15" 34.58 ± 0.27' 30.61 r 0.26' LSI),,,, 0,56 1.52 1.29 CTI 5.4 ± 0,26" 29.6 ±0,17" 25,63 ± 0.50" CT2 5,57 ±0,15'' 40.33 cO-lS*" 38.43 ± 0.25'" C13 7.4 ± 0,20' 54 I 0.36' 43.7 ± ' LSD(, 0^ 0,64 0.74 0,99 CT3 C;'/j;" c/i;r cdc sd cd chir u.h.c (iheo cdt) khdng cd susai khdc cr mircy nghTa 0.03 Kc'l qua d biing eho Uu^v pH diil Ui 4,8 - 8,9, vdi nong ,As dit ki I43,85ppm cav S:iv viii cd thd song va sinh trudng bink thudng I'liy nhien, d mdi trirdng dit cd pi I khiic thi kkii niing sink trudng ciia cav Siiy cimg khae pH cang liing tki sir sink tnidng cua cay siiv ciing giam va ngirgc lai pH cing thip thi sir sinh tnrang ciia Ciiy Siiy ding tiing cu (hi: ckicu cao ciui cay cao nhat dat"36.9 cm o CTI (pFl = 4.8) va ciiy thip nhit la 34,13cm a CT2 vdi pH = 6.9 Tirmiii ttr so ciiv nhidu nhit ki 6.1 diy d CTI vi Ikifp nkit ki 4.7 cav g C T ; ckidu dai ki dai nkit la 33.64ciii d CT I ngin nhit la 30.61 cm o C I diil cluia As, Ngugc lai d tki nghiem diil ckira Pb thi pll cang tiing thi sir pkat tricn cua cay cimg cang liing va pll giam tki sir sink tnidng va phiit trien cua cay say cimg ciing giam cu the: chieu cap cua cay cap nhat dat 54cm sd ea\ iikieu nkat la 7.4 sd la dai nhat la 43.7 cm (deu tuang irng vdi CT3 pH= 8,9) Trong dd chieu cao thiip va ckicu dai ki ngiin nhiit lan lirpl la 29,6 cm va 25.63 cm sd cay it nluit ki 5.4 (deu tuong irng voi CTI pH=4.8) Qua diiy cko tkay mirc dp nhay cam vdi ddc tinh As va Pb eiia cay Siiy cdn phu tkudc vito pH dat va diit d nkiem As va Pbvdi cac mdi trudng pH khac ciiy S;iy viin cd the sdng va sinh tnrdng tdt Ddnh gid khd ndng tich Idy KL.\' cda edy Sdy mdi trirdng pH khdc nhan Bung Hiim lirgng Is Pb lich liiy Inmg thdn Id vd re cuu cdy Sav va;; thdng Irdng ircmg mdi inn'mg pH khdc nhuu iii Meun - Sdi Hiim luong Pb re (ppm) Cil 20,12 ± 0.3" 75,90 0.62' Hiim luong Pb than + la (ppm) 25.67 ± 0.96" CT2 14.08 + 0.34'' 57.63 X 2.97' 23.79 ±0.42'' 79.98 ± 2.05'' CT3 13,92 ±0,18' 48.75J: 1.502' P ± 1.24' 87.16 ± 1.456' 0,85 5.9T 2.84 6.38 Cong thirc sn,, Ham lirong As Irong thiin -¥ la (ppm) Hiim Iirong ,\s re (ppm) 94.3 i 2.65' C;'/i; chii: cdc sd cd chir a.h.^ nheo cot I khdng cd sir sui khdc ir mirc y nglvu 0.03 103 Tran Thi Pha i.; £),' Tap chi KHOA HOC st CONG NGHF BanE\ Khu nJif-z xir ly -!.> va Pb ddl c Bat chira \s itpH Tririfc klii irdng Cong thirc 90(02): 101-105 \;';.; ;7/7aH Sau CTI 143.85 14.99-0."9' CT2 143,85 39.49- 0.91'' CT3 143,85 68,61- il,56- ^D,,.,.,; o i l chira Pb CTI 1016.49 S2.49- () s^' CT2 10 16.19 I " I : 0.76'' Cf3 1016.49 32.99T (1.98' Kcl qua phan tich ham lugng As va Pb tich liiy tkiin la va re cua cay say sau tilling trdng dugc the hien qua bang (.)ua bang cko thay cdng tkirc vdi ac mdi trirdng p l l kkac cua ciing mdt none \ s va ndng dp Pb dircrc chpn lam thi nghiem eay Say deu cd kka nang tick liiy \ s va Pb lluin * la va re Nong dp tick luy \s Vil Pb C T U p H 4.8) la cao nhat cu the kam lugng \ s va Pb tkiin - la re kin lugt lil 20.12 ppm 25.6" ppm va "5.90 ppm 1.3 ppm C on luim lugng \ s Pb tkiin lii re lluip nhiii a C l i p H = 8.9) vdi kiim luong tirong irng lii I •!.92 ppm va "5.90 ppm ddi voi \ s va 25.6" ppm 94.3 ppm ddi vdi Pb, Hiim luong \ s va Pb tick liiy re cua cav siiy C.IO hem riit nhieu so vdi thiin la xay a tal ca cac mdi trirdng pH cua cac cong tlure Mac dii kha niing sinh trirong cua ciiy Say o C13 vdi pH 8.9 cao hon o C I I vdi p l l ^ 4.8 nhirng kha nang uch liiy \s va Pb eay Sav o C I I lai cao trdng la I43.85ppm va 1016.49 ppm sau thang trdng mdi trudng dat co pll khac thi hieu qua kiip tku \s va Pb dat cua cay Siy eao nhiit ki o C T I eu tke la: As han diiu lii I43.85ppni xudng cdn 14,99 ppm giam 9,59 lan so voi ban diiu va Pb lir 1016.49 ppm xudng 82.19 ppm giam 2.32 lan so vdi ban dau lluip nhiit la C l chi giam 2.10 lan i i d i t d nhiem \ s va ".64 kin o diit d nhiem Pb so voi ban diiu Tir ket qua tren cko ta tkiiy riing mdi trudng diit cd nong pH cao tki kka nang klip tku \ s va Pb cua cay Say la thiip hon so voi nidi trudng d i t co p l l thiip Hien luong mdi tririing pi I 4.8 tki kha niing hda tan \ s va Pb dat la cao han dan den kha nang hiip thii As va Pb cua ciiv Siiy tdt han KF I \ N Ndng \s va Pb la cao hon so vdi IC A N 11.99 lan ddi vdi As va 4.52 lan ddi vdi Pb thi kha niing sinh tnrang cua ciiv Say van binh IHTII O C I thudng l u y nkien o cac mirc p l l khac Kel qua cho thay o mdi trudng dat co p l l tki kha nang sinh truong cua ciiy Sav cung ik.ip thi kha niing linh ddng cua As va Pb cao khae bieu hien qua cac chi lieu sinh nin cay Say hut diroc \ s va Pb nhieu han truang (sd cay ckicu eao cay ckicu diii liilcr D i i n h gia k h a n i i n g \ i r 1\ K L N t r o n g diit cua Ciiy s i \ t r o n g mdi t r i r d n g diit pll khiic n h a u mirc p l l - 4.8 tkap han a mirc p l l 8.9 - Cay Say co kha nang hap thii \s va Pb dat vcii cae mirc dp khae Cu tke kha Ket qua x u ly \ s va Pb dat cua cay say o Irong cae nidi tnrong co p l l khac nkau dupe the hien qua bang I re la "5.90 ppm a p l l Qua bang cho thay kha nang hiip tliii diit hi - la la 14.1 iS ppm re la 57.6 ^ ppm, son o nhiem \s va Pb cua ciy Sav la rat ldi \ di o pH ^- 8.9 thiin mdl ham IUOIIL; AS va Pb I r o n " diit tnioc la 48."5ppm Kha nang hap thu Pb cua ciiv 11)4 nang hap thu \ s cua cay Say la kha cao cU the la o pi 4.8 than lii la 20.12 ppm va 6.9 than la la 13.92 ppm va re Triin Thj Pha vd Dig Tap chi KHOA HOC i C()NG NGHF Say la a pll 4.8 than -i- la la 25.67 ppm va re la 94.3 ppm a pH = 6.9 than la la 23."9 ppm re la 79.98 ppm edn a pH = 8.9 than + l