HOI NGH! KHOA HQC C Q N G NGHg SINH HOC T O A N Q U O C 20 1>NG DUNG one PHAM VI SINH (SAGI BIO) Ot Xlf LY CHAT Th NUOl LÔ N Ting Thj Chlnh'''' D$ng Thj Mai Anh, Nguyin Th[ Hda, Trin Van Ti,Fa Vi$n Cdng[.]
HOI NGH! KHOA HQC C Q N G NGHg SINH HOC T O A N Q U O C 20 1>NG DUNG one PHAM VI SINH (SAGI -BIO) Ot Xlf LY CHAT Th NUOl LO^N Ting Thj Chlnh' D$ng Thj Mai Anh, Nguyin Th[ Hda, Trin Van Ti,Fa Vi$n Cdng nghi niki tnrdng, Wfn Han Urn Khoa hgc vi Cdng nghe Vi$t Nam TAT Chin nu6i gia sfic gia cim li nginh san lual quan trgng Viet Nam, c6 tdc df phSt t r i ^ nhanh, nhung ciin nSng ni cho mfii tnr&ng Tlieo dv tmh ciia Bg Nong nghiep vi phSt tnen n6ng thSn dSn n5m 2015, din \ hr^ng chit M i rin s2 diiirakiwing 20 irigu lin/nam Trong thinh phin cua chat thai rin chan nu6i Ig Khi COyi'cic vi sinh v$igftybfnh n h u n khulnE.coU (lif- II^MPN/g), Salmonella (ity- liT CFU/g) vd thii khflng duyc xii If hojc sir dvng lim nguin phan b6n khSng qua xd ly si li nhime tic nhSn gSy nh, tr^g MOl cic bitti pbip tkdikdiTidli nguon chit thai rin niy la ii compost d^Dhi^t d^ cao xd IJ phialvn dik k i ^ ph6ng thi n g h i ^ a SO^C thu rfu^c cho thiy, vi|c b6 sung che p h ^ vi sinl hrffng cic phi thii hihi CO nhir lom r? vi vd trill vao qui trinh li si lira tang h i ^ qui xir IJ: d cac miu thi ^ sau tuin xd If, f cic niu dfli chiing miii giim bSn sau 2-3 tuan d M$t dy ciia cic nhom vi khuan gSy ben vkfical eollform diu giim d b theo tbM gian xir lyf Sau tuIn xir 1^ 50°C, d cic mfiu thi nghiem b6 sung nhir kfaflng c6n cic vi khuin giy bfnh kbi dd S mau dii chiing v3n cfln nhi£n ni|lt df ciing dii gian tich chit lutmg mCin hihi co sau tuin u cbo thiy: o cic miu thi nghiem him lirpng hfla tan cao hon tu hon tif 20-30% vi photpho hfla tan cao hon 10-15% so vdi cic mlu dii chiing Til khda: Chi pham Sagi-Bio, chit tbii r i a Bacillus Streptomyces, Salmonella T6M Mt^DAU Ngfnh chSn nuOi cOa Vift Nam If ng^nh sin xuSi quan trgng cS v l tong skn phhm vk vlfc 1dm ch Id ngdnh cd t6c df phdt tiiln nhanh nhifn ddy cOng \k ngdnh gay o nhilm mfi nfng cho moi h nufli Bf NN&PTNT, vdo Ih&l dilm 1/10/2009 cd nu-^c c6 27,6 trifu Ip'n Dy tinh ddn \Qn se ndm 2015 MSi n9m chdn nufll thdi lu^ng chit thdi long 25-30 trifu m3 vd 80% chat thdi long ho$c sir dgng khfing qua xO 1;^ Id nhOng tdc nhdn gdy fl nhlSm mfli tnrdng nghifm trpng Vh kh6i I 30 trifu diiuton,liwmg chdt thdi Id r^ Ifl'n khodng 20 trifu t^n/nfm Thdnh ph^n cOa chat thii r^n t chira rdT nhifu chat hOu co nhtr xenlulcza Qnh bft, protein, cdc vi slnh v|t gdy bfnh nhir vi Sdmondla vi trdng giun sin Vi vfy phdn Ipn n^u si> dyng tn/c ti^p d l bon cho cfy tr6ng se Idr 88 Idm phdl tdn Idy Ian nguAn bfnh ciia cdc anh vft gay hai, phat tdn mijl hoi th6i tnang qud trir gom Mft s6 kit qud nghlSn dru d b f n cho thdy vifc su- dyng chl phSm vi sinh vft d i xi> 1^ ch thdnh phdn bfln hQiu co vira gidm thilu fi nhiem mfll tru'&ng vi>a cung cdp mft \ugng Idn phdn hf cho nflng nghlfp [Trjnh Quang Tuyfn vd cs 2008 Trjnh Quang Tuyen vd cs 2011] Vai trf cua vi slnh vfl qud trlnh u compost Id phdn hu} cdc chit hO-u ca khf phdn glil d l tidu Tuy nhlfn mfii nhfim nhflm vi sinh vft cfl vai trfl kh^c qud trlnh phdn huj nh phdn huy manh tinh l>ft, protein vd nhfim cfl khd ndng phan huy manh xenluloza vd cdc hpp chat \ge phdn huy phy thufc vdo dllu kifn sinh thdi vd thSnh ph^n cdc chit cfi tnang d6ng u [Tang Thj C Trona.qUd trlnh u tg nhlfn cdc VSV cfi sSn chit thdi slnh trudng vd smh cdc enzym phan h CO Idm cho nhift df dfng u tdng Jfn Mfii lop VSV cfi khd nSng smh tnfdng o- nhift toi thilu t6 nhau, ngirti ta chia chOng thdnh nhfim chinh: u>a Ignh (dudi 25°C) ira am (20 - 45"C) vd u-a n nhlft df dflng u tang Ifn cao (trfn 50°C) cac nhfim vi sinh vft ua km (vi rtit, vi nim nam r Sa/mone//a ) sf ngirng hoat dflng ho|c chit dl cht cfin c&e nhflm vi slnh vft chju nhigt vd ua nhif l ^ ^ S t e ^ w "i T '^ '^'^""" " '^"^'^ "''*"' ^^'^'""^ "^ "^ ''"'"^" *^"^^ ^ Sm r l l ' ' ; ^ ! 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KHOA HOC C O N G N G H ^ SINH HQC T O A N Q U C 2013 t^ifn-Han-larTv-Khoa-hQfl-va-eang-nghfe-Vlfit-Nam-M^t ili^ ci'i^ xa khuan c h l p h i m : 4.5 x10l luan Bacillus dgt 4,5 x109 CFU/g, I thi nghigm de xir ly phkn Ign phong thi nghi$m ChCmg (DCI) kg phdn \gn nghl$m ( T N I ) : kg p h i n I f n + lOg d i l p h i m vi sinh Sagl Bio cft(ing2 (DC2): goOg phdn Ign + lOOg t r l u " igW^m fTN3): 900g phdn Ipn + 100g tidu + 10g che p h i m vi sinh Sagi Bio Chung (DCS): gOOg phdn I f n + 100g ro-m ( d t nhfl) nghiem (TN3): 900g phdn \gn + lOOg rcrm (cat nhfi) + 10g c h l pham vi sinh Sagi Bio thf nghifm dugc u trong t u i n o" nhift dg SCC thf nghifm dugc kiem tra djnh kJ t u i n lan (nhift d f , mdu s i c , pH, dfl I m , mill vd m f t dg cfla s6 ,a v d vl s l n h ciia p h f n Ip-n tru-flrc xO ly cho t h i y m f t dg cua cdc vi slnh n Icrn t u o i Id rat cao nhir VSV dSn 10' CFU/g VSV gdy b f n h 0* CFU/g, E.coli 10^ CFU/g vd CFU/g, D d y j d n g u y f n inh mCii h f i th6i vd cdc m i m ho gla sOc K i t qud cQng cho n i m m l c khflng x i l l t h i f n O f I m cua p h i n \gn Id r l t cao f n ttilch hp'p cho cdc vi sinh v f t n gdy dnh h u d n g tdi su-c khde ^t\ tnrflrng khu vgc chkn nufii xi> 1;? chat thdi ran d i d n I'O'ng phdp Ci compost d n phdi I CO c h i t cfl d f I m t h i p d l Idm khoang 50 -55%, t?o d l l u kifn gl nhu- xg k h u l n sinh t r u i n g vk ,ghldn ci>u ndy chflng t f i d f si> d l p h i i t r f n nhu- da trlnh hky ip nghifn cfl'u B i n g K i t qua phSn tfch c h i t l u ^ g mSu phan ign t n i v c xir 1^ Chi tieu phan tfch pH Tflng huu ccf (OM) Ptingsi N ting s i Dfim VSV long sfl hiiu VSV tfing s i ky khf Ting xa lihuan Ting Salmondia Ting N i m m i c Total cdiform Trimggiun dcmv) K i t qua phan tfch 7,14 30.45 0,8 1,36 63,64 1x10° 3x10' 3x10' 1,1 X 10' 11 % % % % FU/g CFU/g CFU/g CFU/g CFU^ CFU/g TnJng/IOgam in cdc m l u t h i n g h i f m t r o n g q u d t r l n h xu> ly phdn \gn bang che p h i m v i s i n h ira n h i f t duge u tfl I m 50°C theo dfl Irong vfing t d n Chflng tfl t i l n hdnh kiem tra djnh kjr hdng t u i n d l danh i tifu miii vk s y gidm t h i k h l i so vfli tnA>c t i n hanh x i l h?, K i t qud du^c trinh hky d Bang ih g l i cdm quan cac m l u thf nghifm qua tr'mh xir li qui mfl PTN S y gidm tlch k h l i u (%) Mui i i^l in iM ua 2tu&n tuin tuin tuin miii thii mui chua mill Itiii mCii khai mill thii mill chua mill Ihii mOi khai mCii Ihii nh9 mill khai ning mil thii nhe mill khai miii Ihii nhe miii khai miii khai mCii khai nh^ mill khai nong miii khai miii khai hitmCil miii khai nh? Hit mill Mill khai hit mii! 0 0 D 12 11 20 10 , 15 tuin tuin 18 25 23 35 20 - 30 3a 39 35 50 32 37 tuin 35 46 41 60 39 50 cho t h i y , qud trlnh phdt sinh mfli d t i t cd cdc m l u thi nghifm sau U i l n fl d i u gidm mgnh so vdi id\ m i u ddi chfl'ng dfl m l u TN2 giam mfli mgnh n h l t so vdi cac m l u khde Sau tuan u cdc c h l p h i m vi sinh miii ^ i i g i n nhir khflng vd x u l t h i f n mfli khai (mCii d f c txung eka NH3), Tilu QC mfli hoi thdi cfl gidm nhifng v i n cfin Sang t u i n thir 3, d eke m l u TN x u l t h i f n mfli khai n i n g iC mfll t h i i gikm mgnh v d cfl t h f m cd mfli khai cOa NH3 Sau t u i n u d cdc m l u TN khflng mfll r cdc m l u OC v i n cfin D l l u ndy cho t h i y , qud trinh p h f n huy cdc c h i t cfi phdn Ip'n m l u n t o d n , dfi d cdc m l u DC qua trinhchua xdy rahflan todn Trong cdc m l u d i i chu-ng d m l u ' iam c h f m n h l t so voi d c m l u DC2 vd DCS, bdi d m l u C d f am cao hon khflng b l sung b l l 1^ s i lirpng xa khuln CFU/g 2,5x 10' 2.5x10' 2§x10° 3x10" 2,5x 10' 3x10' 1.5x10' 7,2x10 4.6x10 K i t qud Bdng cho thiy, s u i t q u f trinh fl xfl' IJ d cdc m l u do! diCrng (DC) khflng t h i y x u l t t dfi d cdc m l u TN dirpfc b l sung c h l p h i m vl slnh thi mat dg xg khuln t f n g mgnh sau tuan CFU/g sau l u i n u d m l u TN2, TN3 vd sau t u i n d mau TN2, d l l u ndy cho t h i y cdc chung xg kh xudl chfi phdm VI sinh smh frifdng t i t d i l u kifn moi tm^ng c h i t thdi Id p h f n Ipn Bang Bg bien d ^ g m ^ df cda vl khuin Badllus ira nhift q u i trtnh xir IJ Miu ™ ngh*n 0C2 TN2 £)C3 TN3 • ThM glan xir ly; s i lugng vi khuln Bacillus tra nhift (Nudi d SfC 48 h) C Ngay diu uiln kiln 1.8x10' 3.4 X 10* 2.3x10' 2.7x10" 2.0x10' 2.6x10" 5,5x10" 3,5 X 10' 4,1 x10° 4,8x10' 1.5x10' 3,7x10' 7,5x10" 5,2x10' 4,5x10° 6,2 X 10= 3,7 xlO" 4,6x10' 2,6 xlO' 3x10' 1,5x10° 3,2x10' 1,5x10" 1,8x10° 2.5x10' 7,3x10' 2,0x10' 5x10" 2,0x10" 6x10' HOI NGH! KHOA HQC C O N G N G H ^ SINH HQC TOAN QU6C 2013 r b i l n dong m f t d f cua nhflm vi khuan Baci/ftjs Vong & tuan u x i T l y ' c h l n a h i r c h S r m u f l l •Ipn-dcrg'crdho t h i y , cdc m l u TN cfl b l sung c h l p h i m vi sinh Sagi Bio mat dg cua chung cfi cao hon so DC, nhifin s v khde bift n f y khflng qud Idn de t h f h i f n ro rang nhif b l sung cdc chflng xg s vdo qud binh xi> ly D i l u n f y cfi till gidi thich la d i l t thai chan nuoi Ip'n, \ugng vl k h u l n I t s i n mfll tn/dng t i / n h l f n (trong d i t nude, khong ) rat cao, p h f n Ign Id m f t mfil tnrdng thich hg'p cho s y phdt t r i l n cfla nhflm v i ' k h u i n ndy,-nen cdc chflng vl k h u l n Bacillus cua c h l p h i m Jinh du-dng v d i chung qud trlnh fl Do v f y , s y sinh tnffi'ng cua chflng trpng qud trlnh fl xi> \i trgi duge nhu- d l l vdi chflng xg k h u l n ciia s i nh6m vi khuin cd t h i gdy b^nh qud trlnh xir ly (CFU/g) Thdi gian fl, tuin E)C1 TNI DC2 TN2 DC3 TN3 Ngfiy dau 1tuin tuin tuin tuin tuin 1,1x10' 1.4x10* 2.4x10* 1,3xltf 4,9xltf 79 1,1x10' 1.6x10* 7x10" 23 17 1,1x10* 5.4x10* 1.1x10* l.txltf 2,3xltf 54 1,1x10* 4,9x10* 760 33 0 •1,1x10* 3,5x10* 2,2x10* 5,4x10' 7,9x10* 76 1,1x10' 3,3x10' 2,3x1(? 49 13 Ngfiy d i u Ibiln tuin tuin tuin 5tu^ 3x10* 8x10' 3x10* 3,2x10' exio' 9xltf 1,5xltf 3x10* 6.7x10' 2x10" 7xltf 5x10" 11 3x10* 6.4 X i t f IxlO' 24 0 3x10* 4.8 X i t f 2x10* 9xltf 2x1^ 20 3x10* 7,9 X l t f 1x10" 76 10 9x10^ 7x10^ 35 37 - y t h i y , cdc chl s6 total coliform vk vi k h u l n Salmonella d cd m l u TN vfi m l u DC deu cfi xu hu-dng ri gian fl Tuy nhlfn d cdc m l u TN thi m f t d f cua chflng giam nhanh hon so vd\ d m l u DC Sau rN total aliform vk Salmonella da b| tifiu dift hodn todn, cfln d cdc m l u DC, v i n cfin nhvng m f t n g ngu-o-ng chp phdp sCr dgng Idm p h f n bdn theo qui djnh cfla B f Nflng nghlfp vd Phdt triln nfing ng p h d n b o n sau xir I j h gla c h i t Ivgmg cua m i u ph3n l?n t r u ^ v^ sau S t u i n XLF ly Donvido % % % % % % % CFU/q CFU/n CFU/q CFU/fl CFU/fl TrOmq/lOg Miu phfin ign trudc xir ly 7.14 rlt Ihdr 30,45 0,33 , 0,8 1.36 0.16 0.36 68,64 1x10° 3X10' 1,1x10° 35 11 M l u sau tuin ii xir ly BC1 7.05 mill hfli 24,7 8.7 0,72 1.1 0.28 0,45 52.3 6,5 X 10° 5,3x10* 79 0 Tf^l 7.1 hetmui 23,8 9,2 0,71 1,05 0,35 0,67 45,7 2.6x 10° 4x10' axlff 11 BC2 TN2 7,1 mill khai 28,6 9,5 0.61 1.02 0.32 0.52 -40 5.5 J( 10° 2X10"' 54 20 7.1 helmCii 25,4 11.2 0.62 0.85 0.45 0.84 33 8x10' 3x10' 1x10° 0 DCS 7.1 mill khai , 27,3 10,4 0,63 1.1 - 0.31 50 40 2x10° 3,5x 10' 76 35 TN3 7.0 hfii mfli 24.9 12.5 0.62 1,0 0.42 _ _ 0.78 34.5 6.2 X Iff 6x10' 5x10' 0 ho t t i l y , h i f u quS ro r f t cua c h l p h i m vi sinh ira nhift Sagi Bio t n sung vao qud trinh u xu- ly d i l t ng d i l u k i f n nhift d f cao, m f t d f cfla cdc vi ^ n h v f t cfi khd nang gay b f n h nhu* cdlforrm Salmonella mgnh v d trif t tieu hodn tofin sau t u i n u Cdc chi tieu danh gid chat lirgng cua mfln hi>u c o (mfln hfia p h d p h o d l tifiu) sau l u i n u d cdc m l u TN d i u cao h e n so vfii m l u d o chfl'ng: d cdc mfiu thi nghifm tan cao h o n tfl- 20-25%, N hfla tan cap hpn tCr 20-30% vd photpho hfla tan cao h a n 10-15% so cdc l l y v i f c b l sung t h f m cdc ccr c h i t Ifi vfl t r i o vd tam rg vdo tfOng qua t i n h fl x d 1]^ se Idm tdng hifu qud 0% vo t r I u CO tdc dgng xi> ly t i l hon so vdi rcrm rg I o h j xO 1^ F^dn Igm d i l u k i f n phfing thi nghifm b 50''C thu d u ^ c cho thay v l f c b l sung c h l tio (difgrc s i n x u l t tCr cdc chung yi k h u l n Bacillus vd xg k h u l n Slreptomyces ua nhift) vk 10% k h l i M h i ccr Id vo t r l u vd rcrm rg vdo qud trinh se Idm tang h i f u qud x d 1;^: d cfic m l u thi nghifm mui I t u i n xiH|?, d cdc m l u d i i chdng mOi gidm h i n sau 2-3 h j i n fl M f l d f cOa cdc nhfim vi k h u l n gdy la, E.cpf vd fecal conform d i u gldm d i n theo thfii gian xG> I j ; Sau t u i n xir ly d 50°C d cdc m l u till HOI NGHI KHOA HOC cONGNGHeSINH HPC T O A N QUOC 2013 ^hii„h4s„ng^phi.^B»ri^h.«n|»„,^^^^^^^^ nhung mil JO a i giim aanj ki Cac Chung xa khuSn MJ ™"9 " cac mau ail chiing xs khuan Chung in B vi ph« hiln " j ^ " * ' " ' S V c h S j ^ ^ c iSu mi nghiim ham lii,ng miln LMcimorn CUV nil}' TAI LI^U THAM KHAO ABasRM., Brown A.E:,MilerW.L (1998) Experimedd Micmbiology Fundamentals and Applicatran, Macmi New York 16ng-nHChl,ii{2O05) COnanghfxdlfchithOucaeiiaraethiisinhhoatbingvisinhvatuanhiftTuykntkp Hfi nghi mfll truimg tofin quic 2005, tr 97^987 Tfing -mi Chlnh OSng Dinh Wm Phan Thi Tuyit Minh Le Thanh ^f^^°°^j-^Shidnc^sAnxuil vi dng dun Blnh v4tdSxdl^ chit Ihi, hOu CO TC Khoa hpc-OHQGHN, KHTN&CN, tap XXil, No3B PT, tr 38-44 HeshamM Abdulia (2007) " enftancement of rice straw composting by lignocelluloselytic Aetinom Agriculture & biology S (1), pp 106-109 Schonbom W (1986) iW/croWa/ Degradation Biotechnology (ed H J Rehn and Reed G ), voi.8,362-398.500 -57 Tfinh Quang Tuyfai, Nguyin Qui Cfll NguySn Thi Binh, Nguyin Tiin Thflng, Bfim Tuin Tii (2008) Thgc trang X(> V chit thii bong chan nudtontrang tid tip ming Bfio c^o khoa figc nam 2008 B^ NN&PTNT, Vifn chfin nuf Trinh Quang Tuyfin vd CS (2011), M ^ s i gid phip xd ty phin vd nude Ihii nh^m giam nh$m mdi tmdng tror tral t$p tmng TC khoa hoc cflng nghf chan noW - B^ Nflng nghiep, s6 28, thSng 02/2011; tr.55-70 THE APPLiCATION MICROBIAL PREPARATION (SAGI BIO) FOR CC SOLID WASTE OF PIG BREEDING Tang ThI Chlnh*, Dang Thi Mai Anh, Nguyeh Thi Hoa, Tran Van Tua Institute of Envimnmental Techndogy, Vietnam Academy of Science and Technology SUMMARY Breeding cattle and poultry is an inqwrtant agriculniral sector in Vietnam, develops very fast but causes a pollution In accordance to Ministry of Apiculture and Rural Development, in 2015, the total quantity of pig is mitions and the solid waste is estimated to be 20 millions tons/year In the solid waste of pig breeding, the sulwtances and microorganisms which can cause disease, such as E coli (lif - lO^MPN/g), Salmonella (lif-10 eggs, if those wastei are not treated well or used as fertilizer without appropriate treatment, they can cause ; pollution Therefore, one of the best solutions for solid waste treatment is composting those wastes und Moreover, to raise ttie efTiciency and effecBveoess of the treatment, we can add Sagi Bio preparation and 10% o as straw and husk to the composting procedure The result shows that: for the Sagi-added sample, bad smell de week of ireatmenl; while for the control -sample, it took 2-3 weeks Io get the same result Importantly, the densi such as Salmonella E.coli and fecal eoUform decreased widi the treatment time; after weeks of treatment at added sample, almost disease bacteria were eliminaled, while for the coattol-sample, the density of disease b disease bactena were still available In the bottom line, the analysis of the quality of composting product afte for Sagi- Bio added sample, the amount of soluble mulch are 20-25% higher, soluble nitrogen 20-30% higher an higher than for control-sample Keywords: Microbial preparation Sagi-Bio soUd waste Bacillus, Streptomyces, •Author tor correspondence: Te); +84^04187106; Email: langthichmh@gmail.com Salmonella ... fl, nen u-c c h l si^ phdt triln cua vi k h u l n kj I m l u TN b l sung c h l p h i m vi sinh cQhg Idm gidm so luvng cua vi khul''n kl so vdi m l u DC ciing sinh v f t h i l u phdl triln mgnh s... p h i n I f n + lOg d i l p h i m vi sinh Sagl Bio cft(ing2 (DC2): goOg phdn Ign + lOOg t r l u " igW^m fTN3): 900g phdn Ipn + 100g tidu + 10g che p h i m vi sinh Sagi Bio Chung (DCS): gOOg phdn... COnanghfxdlfchithOucaeiiaraethiisinhhoatbingvisinhvatuanhiftTuykntkp Hfi nghi mfll truimg tofin quic 2005, tr 97^987 Tfing -mi Chlnh OSng Dinh Wm Phan Thi Tuyit Minh Le Thanh ^f^^°°^j-^Shidnc^sAnxuil vi dng dun Blnh