KHOA HOC CONO NGHE PHAIM LAP, TUYEM CHOIV VI KHUAIV PHAIV G! Al XEIVLULD OE XLT LY BA THAI OOIVG RIEIVG T H A M H PHAM HOLJ CO Phan Qudc H u n g '''' , Vu T h i Xuan Huong^, Ngu> dn Thd Binh'''' '''' T6MTAT Ca[.]
KHOA HOC CONO NGHE PHAIM LAP, TUYEM CHOIV VI KHUAIV PHAIV G! Al XEIVLULD OE XLT LY BA THAI OOIVG RIEIVG T H A M H PHAM HOLJ CO P h a n Q u d c H u n g ' , Vu T h i X u a n H u o n g ^ , Ngu> dn T h d Binh'' T6MTAT Cac VI k h u a n p h a n giai xenlulo da dupc phan lap tuyen c h p n tir ba thai s a n x u a t tinh h o t d o n g HL-ng (Canna edulis Ki-r.) va dat xung quanh khu vuc lay mku tai cac lang nghe che bien tinh bot dong neng a s \A thupc linh ihanh la Ha Ni>i Hung Yen, Quang Ninh nhani thu thap nhmiu vHun? vi khuan cti kha nftng phan Riai xenlulo manh &k xir ly ba thai dong ri^ng noi n e n g va rac thai in i< noi chung Tu mau ba Ihai dong neng va m^u dal quanh khu vuc lay m i u ba thai da phan lap duoc 30/42 chiinR VI khuan co kha nang phan giai CMC va tuyen chon dupc 02 chting vi khuan gom VKl va XT^S co kha nang phan huy chiiy, , a , „,-,„, , „ phaha, - ' ' " "" *>"' 1° ng 10' w 10-' trai deu Iren moi lmmg; u m:; cac khuan lac rieng bit-l d, • •>" L Lira cijon Iron irong ong ngh.em va coi ,!„ „h„ L *'" ^ o n g biel biel """ ^ i"'i>^ '^ la kha nang doi khang cua vi khuan cbonS ''" ^ tiet CP ban chat la khang sinh ciia cac \od\ kliac n 'Uig mdi truong sdng Kdt qua danh gia nurc Khang khang sinh Sti-eptomycin vdi cac ndng khac ciia cac chiing vi khuan Ihe hien d- b;mg Bang cho thay ca chting vi klui.i.i ddu cd klia nang khang khang sinh b mtic dp khac nhau, mat dat tTj 9,6 den 54,5 CFU/ml Tu> nhion b mtic khang sinh cao tJii mat dp vi khuan co xu htrong giam 3.3 Tuydn chpn to hpp vi khuan cd hpat tinh sinh hpc cao va san xu^t chd ph&i smh hpc dd xii ly ba thai dong ridng 3.31 Tuyen chpn tdhpp vi khuan Tren ca sd danh gia cac dac tinh sinh hpe 02 chiing VKl, VK28 cd boat tinh enzym phan giai xenlulo manh, cd thai gian mpc nhanh, kich thudc khuan lac ldn, thich ung pH rdng, chiu nhiel tdt va kha nang khang khang sinh cao dupc luyen chpn de san xual chd pham sinh hoc dung cho xu ly ba thai dong ndng 02 chiing vi khuan da tuyen chon dupe ihii Unh ddi khang, Cac thdng sd ca ban vd 02 chiing \i khuan du()c the hien a bang Bang B$c di^m cua 02 chiing vi k h u ^ dupc li^ chpn san xuat chd pham Thdi Nhdm Kyhidu I „Dac diem - ,., _,„ ; HinhUiai gian khuan lac pH te bao gram •luing mpc SIT (°C) (gid) \'K2S Tron trang nga vien rang I cini be mat nhan ! Tron U';iiig nga vien ddu be mat iron 3.32 Ihnh.a.i char hnmgdn-plum smb hoc Chl tieu [PHK^ Oo am "vKi" \'K2.s \"S\ Don vi linh pham sinh % 33.5 lo-crr/KC 9.4 To"-ci'i 'IC\'Nhl'> -2(K)2 io- 7.(1 1.5 15 > 1,0x10" CFU/g > 1.0x10" CFU/g Xti ly ba thai dong ridng bang che pham sinh So heu a bang chi rp: Sau 25 it, ba thai dong ridng d cdng thtic xu ly ehd pham sinh hpc ed hoc cd tac dung day nhanh tdc dp miin hda, nit ngan mau den, hoai muc va xdp han ddi chiing SP vdi ket dupc thdi gian ii so vdi ddi chiing (7 ngay), dae bidt qua nghien ciiu ctia Nguyen Ngoc Quy, Ta Tliu san pham sau ii cd cac tidu ehi dat Nghi dinh Hang, Le Tai Khuong, Doan Van Tii (2016) thi thai 108/ND-CP 20/9/2017 cua Chinh phii vd quan gian u ngan hon ngay, nhung nhiel Max cua iy phan bdn nen ed the dting lam phan hiru ca bdn ddng u thap hon 6'^''C Nhu vay che pham sinh hoe cd cho cay ti-ong tae dung thiic day qua trinh miin hda va lain tang TAI LEU THAM KHAO ham lupng dinh dupng tao (OC giam 24,5%) Nguydn Ngoc Quy, Ta Thu Hang, L^ Tat Cac chi tieu vd mat dp vi khuan cd ich, pH„2o dp am Khuong Doan Van Tu (2016) Nghien eiiu xii ly ddu dat yeu cdu so vdi tieu chuan quy dinh tai Nghi ngudn ba thai dong ridng phan hiiu cavi sinh dinh 108/ND-CP 20/9/2017 eua Chinh phd vd bdn cho cay dong ridn Tap chi Ndng nghiep va quan ly phan bdn nen cd the sir dung san pham nhu PTNT, Sd 6/2016 mdt ngudn dinh duong de bdn cho cay ti'dng Nguydn Thi Thu Thuy, Nguydn Tidn Long, KET LUAN Tran Thanh Dtic (2018) Phan lap, hiyen chgn va Ttr S mau ba thai dong ndng va mSu dat quanh dinh danh vi khuan cd kha nang phan giai xenlulo dd khu vuc lay mau ba thai da phan lap dupc 30/42 san xuat phan hiiu ca vi sinh Tap chi Khoa hpc - Dai chiing vi khuan ed kha nang phan giai CMC va tuyen hpc Hue, ISSN 2588-1191 Tap 127, So 3A, 2018, Tr chpn dupc 02 chung vi khuan gdm VKl va VK28 cd 117-127 kha nang phan huy, chuydn hda bdt gisiy (dat 58,8% Nghi dinh 108/ND-CP 20/9/2017 ciia 61,4% tuang ling) phan giai ba dong ndng (dal 58.2%; Chinh phii vd quan ly phan bdn 60,2% tiJong ling) de lam gidng san xuat chd pham Robert Rynk (editor) (1992) On-farm sinh hoc dat TCVN 6168-2002 composting handbook, Publish by thaca, N Y.: NONGNGHIEPVA PHAT TRIEN NONGTHON - KY - THANG 10/2019 65 •ill: KHOA HOC CONO NCHE ung vol pH moi truong ciia cac chiing \n khuan dupc tnnh bay db.-mg Tat ca chiing thi nghiem deu co kha nang sinh Inning, phat men khoang pH tu ; ^ Mn mat dao dong tir 21.5- afi.LNlO'CFU/ml chiing to chiing VI k h u ^ co kha nang thich ung rpng Dieu sc CO y nghia i-at km u-ong viec ung dung cac chung giong dc san xuat che pham xir ly phe thai hiru CO ran Theo R Rynk el al (1992) khang dinh ffong qua tnnh li phan htru co pH aia {tone li thuong dao dpng tu 5-9 3.i'.3 Klia nang khang khang sinh ciia cac chung VI khuan Bang K6t qua dinh gia kha nang khing khang sinh aia cic chimg vi khuan Ky M.il (I.i \ S \ l\lii c n lull STI"; hieu 300 500 8(K1 KKIO Cllllll.g ,'l) (mc 1) (iiic,ll Cing/1) \K1 32.7 , 'I.,; f VK24 \1 khuan u-ong moi truimg tu nhien t)a\ ,iin.g cl'nih la kha nang doi khang ctia vi khuan ch.'iic la' ^'I'^t uet CO ban chat la khing sinh ciia cic Ioai kliac uong moi tnrong sdng Ket qua danh gia nuic l^hang khing sinh Sheptomycin voi cic nong khac aia cac chiing vi khuan the hien o bang i! Bang cho thi'y ci chiing vi khuan deu co nang khang khang sinh muc kh.u mat dat hi 9.6 den 54.5 CFlI/ml Tii.\' iiliicn o rnirc khang sinh cao thi mat vi khuan co xu huong giira 3.3 Tuy^n chpn hpp vl khu& c6 hoat tinh smh hpc cao vi sin xuSit ch^ phSra sinh hpc d^ xir ly bi thai dong ri^ng )'.•)' / TuyCn ebon td hop vi khuan Tren CO so dinh gii cac dac tinh sinh hpc 02 chung VKl VK28 co hoat tinh enzym phan giai xenlulo manh co thoi gian moc nhanh kich thuirc khuin lac lon thich img pH rong, chiu nhiet tdt vi kha ning khing khang sinh cao duoc tuyen chpn d^ sin xual che pham smh hoc diing cho xir ly ba thai X\\1X dong neng 02 chiing vi khuan da hiyen chpn duor Khang khang sinh Ii mot nhung die Unh thir hnh ddi khing Cac thdng sd co ban ve 02 chung quan trpng danh gia kha ning ton tai canh tranh cua \i khuan dupc Ih^ hien d bang _._ Bin^ D jc di^m ciia 02 chung vi khuin dugt lua chpn sin xuit chJ' ph jm Thdi Kvl Nhiet Hmh tJiai Nhdm gian Dac diem khuan lac pH dd te bio gram moc fC) Jgio) Tron ti-ing nga Hmh que, 24 cua be mat nhan CO nha bio 20-60 Tron tring nga Hmh que mit tron CP nha bao 2a60 3.3.2 D.-inh.i.na dial linmg chr ph.un yinli bo, Bing Chit lupng ch^ pham smh jipc Che : Chi lC\"\hI(t pham tieu -2002 sinh 6^1"-CFU/g ddi N O N G NGHIEP VA PHAT TRIEN NONG THON - KY - THAN 10/21119 KHOA HOC CONG NGHE vol M\l Trong dd, vi sinh vat lap chi chiem 1,596 (Udu chuan cho cho phep < 5%) 3.4 K^t qua thir nghidm xii ly ba thai dong rieng bang ch^ ph3m vi sinh vat Chd pham sinh hoc sau che lao dat tieu chuan cho phep theo Tieu chuan Viet Nam TCXTs 6168-2002 da duoc ling dung h'ong xu iy chat lliai tir boat ddng san xuat tinh hot cii dong ridng voi quy mo ddng li dat 100 kg ba thai Cac chi Udu theo doi, danh gia hidu qua xii ly cung nhu chat luong san pham dupc Uinh bay d'bang Truoc u Chi lieu t)d am pH„,„ Nhiet dd max Don vi tinh % °C Sau ii DC Xirly DC Xirly 61.52 6.2 60,13 6,2 39,41 4,8 25 25 24,13 5,8 62 Ddhoai Mau sic Ham luong chat hihi co Mat vi khuan VKl Mat vi khuan VK28 % 48 Chua hoai Hoai Ving xam Ving xim Xim niu Den, toi, xdp 48.15 48,34 46,37 36,50 XlO'* CFU/g XlO" CFU/g So lidu d' bang chi ro: Sau 25 u, ba thai dong rieng d- cdng thuc xii ly che pham smh hoc cd mau den, hoai muc va xdp hon ddi chiing So vdi kdt qua nghien ciiu cua Nguydn Ngoc Quy, Ta Thu Hang, Le Tat Khuong, Doan Van Tii (2016) thi Uioi gian u ngan hon ngay, nhung nhiet Max ciia ddng u thap hon 6"C Nhu vay che pham sinh hgc cd tac dung thiic day qua Irinh miin hda va lam tang ham lupng dinh duong lao (OC giam 24,5%) Cac chi tieu ve mat dp vi Idiuan cd ich, PHH2O, am deu dal yeu cau so vdi tieu chuan quy dinh tai Nghi dinh 108/ND-CP 20/9/2017 cua Chinh phii vd quan ly phan bdn ndn cd the su dung san pham nhu mdt ngudn dinh duong de bdn cho cay trdng KET LUAN Tir mau ba thai dong ridng va m^u dat quanh khu vuc lay mau ba Uiai da phan lap dugc 30/42 chting vi khuan cd Idia nang phan giai CMC va tuyen chpn duoc 02 chiing vi khuan gdm VKl va VK28 cd kha nang phan hiiy chuyen hda bpt giay (dat 58.8%; 61,4% lirong ung) phan guii ba dong rieng (dat 58.2%; 60,2% hrong ling) de Iam gidng san xuat chd pham sinh hpc dat TC\^ 6168-2002 3.8 4.5 Tieu chuan phan hiru CO vi sinh theo ND 108/ND-CP 5,0 >15 > 1,0x10" CFU/g > 1,0x10" CFU/g Xu ly ba thai dong ridng bang chd pham sinh hoc cd tac dung day nhanh tdc dp miin hda, nit ngdn dupc thdi gian li so vdi ddi chiing (7 ngay), dac bidt san pham sau li cd cac Ueu chi dal Nghi dinh 108/ND-CP 20/9/2017 cua Chinh phii ve quan ly phan bdn nen cd the dung lam phan hiiu ca bdn cho cay TAJ LEU THAM KHAO Nguydn Ngoc Quy, Ta Thu Hang, Le Tat Khuong, Doan Van Tii (2016) Nghien cuu xii ly ngudn ba thai dong ndng phan huru cavi sinh bdn cho cay dong ridn Tap chi Ndng nghidp va PTNT, Sd 6/2016 Nguyen Tlii Thu Thiiy, Nguyen Tidn Ixng, Trdn Thanh Diic (2018) Phan lap, tuyen chpn va dinh danli vi khuan cd kha nang phan giai xenlulo de san xuat phan huu ca vi sinh Tap chi Khoa hoc - Dai hoc Hue, ISSN 2588-1191 Tap 127, So 3A, 2018, Tr 117-127 Nghi dinh 108/ND-CP 20/9/2017 cua Chinh phii vd quan ly phan bdn Robert Rynk (editor) (1992) On-farm composting handbook Publish by thaca, ,\.Y, N O N G NGHIEP V A PHATTRIEN N O N G THON - KY - THANG 10/2019 65 ^ KHOA HQC CONG NGHE Northeast Regional Agncultural S'l-v ice Cooperative Extension ^^^^.^ , Engineenng Tieu chuan Viet Xnni TC\'N 616^ ^00-''' ^ ^^ P^^^' ^"i "^^^ vat phan giai xenlulo Ryckeboer Jaak & Mergaert, J & \'aes K & Klainmer S & De Clercq, Deborah '^ Coosenians j ^ Insam Hcnbert & S\\ ings Jean (2003) A sun'ey of bactena and hingi occumntr dunng composUng and self-heaung pmccsses Annals of Microbiologj', V()1.53.pp.31^Ml(i " Vo Van Phudc Que Cao Xgcu RieP (-^^^^• Phan lap va nhan dien vi khuan phan riai cellulose Tap chi Khoa hpc 18a tr 177-I,S-1 g_ William M Fogarty, (198:i) Microbial enzymes and biotechnology Applied Science Pubbshei-s ISOLATING AND SELECTING CELLULOSE DECOMPOSITION BACTERIA TO Tl^VL RESIDUES OF GALANGAL TUBER'S STARCH TO MANURE Phan Quoc Hung, Vu Thi Xuan Huong, Nguyen The Binh Summary Cellulose clecomiwsinE bacu-na wcrt" isolak-d selectedfrom'^e residues of galangal tuber's starch (Canna filuti.- KIT) and the soil surroundinK the samplinR area in the residues of processing villages in I'diiummes of i>rovinees aiul cilies are Ha Noi HiinR Yen and Quang Ninh in order to collect bactena wiUi cirong ccliulosc resolution ability to ireal waste nl galangal tuber's starch in particular and organic waste in general From S samples of residue and soil samples around Ihe area of coHecUng waste samples, 30/42 stramsof CMC-eapablebaclena were isolated and two strains of VKl and VK28 were capable of separating, desiroyiiig and convening pulp (reaehing 58.8%; dl 4it respectively), decomposing debris (reaching 58.2%; (i() 2^1 respectively) lo miike breeding vaneUes thai produce bio-producls TCVN 6168-2002 Treatment of waste of galangal tuber's starch wiUi biological products has the effect of accelerating the speed of humus, shortening the mcubalion time compared lo the control (7 days), especially after the incubation products meet the cnlena of Deeree IDS/ND-Cr dated September 20, 2017 of the Govemment on fertilizer management, so il can be used as organic fertilizer for crops Keywords: Bactena decompt'^iluui cellulo r: galangal residue, probiolics Ngu^i phan bien: PGS.TS U Nhu Ki^u Ngay n h ^ b a i : Ki/S/liOh) Ngay thdng qua phan b i ^ : 13/9/2019 Ngay duydt dang: 20/9/2019 NONG NGHIEP VA PHAT TRIEN NONG THON - KY - THANG iQ/^mc ... Iui>t''n BaVi.TP.HaNoi Mau ba thai dong ru Mmh Quang, huyen Ba ''i, Tl la Noi Mau ba thai dong rieng xa Minh Quang, huyen Ba Vi, TP H;: Noi Mau ba thai dong neng \a Minh Quang, huyen Ba Vi I''F 1-ia... trang nga vien d^u b6 mat tron 24 Tron vang dam, vi^ n deu be mat loi nhay Tron vang nhat, vien d&u hk mat tron loi nhay Tron trang sira vi^ n deu b6 mat tron 16i, nhay Tron, trang nga, vi4 n dSu... thai dong ndng va mSu dat quanh dinh danh vi khuan cd kha nang phan giai xenlulo dd khu vuc lay mau ba thai da phan lap dupc 30/42 san xuat phan hiiu ca vi sinh Tap chi Khoa hpc - Dai chiing vi