151 Liposomal Surface Coatings of Metal Coronary Artery Stents for Efficient Non Viral Gene Delivery to the Injured Vasculature Molecular Therapy Volume 20, Supplement 1, May 2012 Copyright © The Amer[.]
CARDIOVASCULAR GENE & CELL THERAPY Due to the extremely short half life of prostacyclin in plasma (42 seconds), it is usually measured indirectly by measuring its stable metabolite, 6-keto-prostaglandin 1α(6KP) Measurements were performed 48 hours after hydrodynamic gene delivery by delivering a fatal dose of ketamine and drawing blood via cardiac puncture 6KP was extracted from plasma using a C18 column and detecting with a 6KP ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) Results: 6KP levels in the plasma of Cox-1/PGIS treated animals exceeded 18pg/ml, as compared to 2pg/ml for control animals By comparison, 6KP levels in humans, reported in a previous study, were