122 Efficient and Sustained Gene Transfer to Neuroepithelial and Neuronal Cells Following Intratechal Injection of HDAd Vectors Molecular Therapy Volume 17, Supplement 1, May 2009 Copyright © The Amer[.]
ADENOVIRUS VECTOR BIOLOGY pIX-mRFP1 and pV-EGFP by simultaneous detection allowed us to observe the complete cycle of viral replication in infected cells These results indicate that the simultaneous detection of adenovirus using fluorescent proteins is suitable for real-time analysis, including identification of infected cells, and monitoring viral spread, which will be required for evaluation of oncolytic adenoviruses In conclusion, the dual-labeling of adenovirus using two kinds of fluorescent proteins offers a unique way to more thoroughly monitor adenovirus infection and to study adenovirus biology that corresponds to the site of BMP-4TK injection in mice treated with gancyclovir 120 Regulated BMP-4 Expression for Bone Regeneration by Thymidine Kinase and Ganciclovir Treatments The renin-angiotensin system (RAS), has multiple regulatory functions, as well as pathophysiological effects in cardiovascular disease (CVD) such as cell proliferation, cardiac hypertrophy, inflammation and oxidative stress via the actions of angiotensin II (AngII) Angiotensin converting enzyme (ACE2), a homologue of ACE, whose expression is restricted to heart, testis and kidney, metabolizes AngII to Ang1-7, suggesting the presence of local tissue-specific RAS Recent studies have indicated that Ang1-7 antagonises AngII signalling via the receptor MAS, indicating that maybe Ang1-7 is a useful therapeutic for CVD We used exogenous peptide and a gene transfer approach to study Ang1-7 effects in cardiomyocyte hypertrophy We developed adenovirus vectors that over-express Ang1-7 using an expression cassette encoding a renin signal peptide (to ensure secretion), a mouse heavy chain IgG (to provide mass for efficient production of the protein), a furin protease cleaveage domain (to release the peptide) and Ang1-7 Efficient expression of the Ang1-7 fusion protein in RAdAng1-7 transduced cardiomyocytes was confirmed by western blot To study each peptide in cardiac hypertrophy an in vitro model was established in rat H9c2 cardiomyocytes and primary rabbit cardiomyocytes H9c2 cells were stimulated with 100nM AngII for days to induce cellular hypertrophy The length of AngII-stimulated cells was 149.7±15.6nm (unstimulated = 112.3±13.5nm; p