Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.

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Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.Nghiên cứu hệ gen ty thể, đơn vị mã hóa ribosome của sán lá phổi họ Paragonimidae tại Việt Nam và một số khu vực châu Á.

BỘ GIÁO DỤC VÀ ĐÀO TẠO VIỆN HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM HỌC VIỆN KHOA HỌC VÀ CÔNG NGHỆ - Nguyễn Thị Khuê NGHIÊN CỨU HỆ GEN TY THỂ, ĐƠN VỊ MÃ HÓA RIBOSOME CỦA SÁN LÁ PHỔI HỌ PARAGONIMIDAE TẠI VIỆT NAM VÀ MỘT SỐ KHU VỰC Ở CHÂU Á LUẬN ÁN TIẾN SĨ SINH HỌC Hà Nội – 2022 BỘ GIÁO DỤC VÀ ĐÀO TẠO VIỆN HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM HỌC VIỆN KHOA HỌC VÀ CÔNG NGHỆ - Nguyễn Thị Khuê NGHIÊN CỨU HỆ GEN TY THỂ, ĐƠN VỊ MÃ HÓA RIBOSOME CỦA SÁN LÁ PHỔI HỌ PARAGONIMIDAE TẠI VIỆT NAM VÀ MỘT SỐ KHU VỰC Ở CHÂU Á Chuyên ngành: Hóa sinh học Mã sỗ: 42 01 16 LUẬN ÁN TIẾN SĨ SINH HỌC NGƯỜI HƯỚNG DẪN KHOA HỌC: GS.TS Lê Thanh Hịa TS Đồn Thị Thanh Hương Hà Nội – 2022 iii LỜI CẢM ƠN Để hồn thành luận án này, tơi xin bày tỏ lịng biết ơn sâu sắc tới: - GS.TS Lê Thanh Hoà, ngun Trưởng Phịng Miễn dịch học, Viện Cơng nghệ sinh học, Viện Hàn lâm Khoa học Công nghệ Việt Nam, người thầy rất tâm huyết tận tình trực tiếp hướng dẫn, định hướng nghiên cứu, tạo điều kiện tối đa truyền dạy kiến thức, kinh nghiệm, động viên giúp đỡ tơi q trình học tập, nghiên cứu hoàn thành luận án - TS Đồn Thị Thanh Hương, Trưởng phịng Miễn dịch học, Viện Công nghệ sinh học, Viện Hàn lâm Khoa học Công nghệ Việt Nam, người thầy, người chị tận tình dạy đồng hướng dẫn khoa học thời gian học tập nghiên cứu Tôi xin gửi lời cảm ơn chân thành nhất tới đồng nghiệp Phịng Miễn dịch học Phịng thí nghiệm trọng điểm Công nghệ gen, Viện Công nghệ sinh học, Viện Hàn lâm Khoa học Công nghệ Việt Nam nhiệt tình tạo điều kiện giúp đỡ tơi hồn thành luận án Tơi xin trân trọng cảm ơn: - Ban lãnh đạo Viện Công nghệ sinh học, Viện Hàn lâm Khoa học Công nghệ Việt Nam tạo điều kiện cho học tập nghiên cứu - Ban Giám đốc, Khoa Công nghệ sinh học, Phòng Quản lý Đào tạo - Học viện Khoa học Công nghệ tạo điều kiện giúp đỡ tơi suốt q trình học tập - Cám ơn hỗ trợ kinh phí Quỹ Phát triển Khoa học công nghệ Quốc gia (NAFOSTED) đề tài mã số 108.02-2017.09 108.02-2020.07 GS.TS Lê Thanh Hòa chủ nhiệm - TS Đặng Tất Thế, Viện Sinh thái Tài nguyên sinh vật, Viện Hàn lâm Khoa học Công nghệ Việt Nam; GS.TS Takeshi Agatsuma, Trường Đại học Kochi, Nhật Bản; GS.TS David Blair; GS.TS Nguyễn Văn Đề, Trường Đại học Y Hà Nội PGS.TS Phạm Ngọc Doanh, Viện Sinh thái Tài nguyên sinh vật, Viện Hàn lâm Khoa học Công nghệ Việt Nam, giúp đỡ hợp tác trao đổi thu mẫu, thí nghiệm nghiên cứu hình thái học cho nghiên cứu giúp đỡ khác công bố hồn thành luận án Cuối tơi xin bày tỏ lịng biết ơn sâu sắc tới gia đình chỗ dựa vững chắc, giúp đỡ chia sẻ khó khăn cho tơi suốt q trình thực luận án Tơi xin chân thành cảm ơn chồng khún khích truyền nhiệt hút cho tơi thêm sức mạnh để giúp tơi hồn thành q trình học tập nghiên cứu Hà Nội, ngày 13 tháng năm 2022 Tác giả Nguyễn Thị Khuê LỜI CAM ĐOAN Tơi xin cam đoan: Đây cơng trình nghiên cứu một số kết cộng tác với cộng khác; Các số liệu kết trình bày luận án trung thực, một phần cơng bố tạp chí khoa học chuyên ngành với đồng ý cho phép đồng tác giả; Phần lại chưa cơng bố bất kỳ cơng trình khác Hà Nội, ngày 13 tháng năm 2022 Tác giả Nguyễn Thị Khuê MỤC LỤC Giới thiệu ty thể, nguồn gốc, cấu tạo chức Cấu trúc sắp xếp gen hệ gen ty thể Mitoribosome của ty thể thành phần cấu tạo Đặc điểm gen học hệ gen ty thể Tr i iii iv viii xi xiii 3 13 18 21 21 23 26 26 Cấu trúc đơn vị mã hóa ribosome của hệ gen nhân 31 Lời cảm ơn Lời cam đoan Mục lục Danh mục chữ viết tắt Danh mục bảng Danh mục hình MỞ ĐẦU CHƯƠNG TỔNG QUAN TÀI LIỆU Tình hình nghiên cứu sán phổi họ Paragonimidae 1.1 1.1.1 1.1.2 1.1.3 1.1.4 1.1.5 1.2 1.2.1 1.2.2 1.2.3 1.2.4 1.3 1.3.1 1.3.2 1.3.3 1.4 Phân loại sán phổi họ Paragonimidae Phân bố địa lý sán phổi họ Paragonimidae Đặc điểm sinh học sán phổi họ Paragonimidae Tình hình nghiên cứu sán phởi họ Paragonimidae giới Tình hình nghiên cứu sán phổi họ Paragonimidae Việt Nam Giới thiệu ty thể, ribosome của ty thể hệ gen ty thể Cấu trúc ribosome của tế bào Đơn vị mã hóa ribosome của hệ gen nhân tế bào Nghiên cứu ứng dụng chỉ thị phân tử của đơn vị mã hóa ribosome Sư cần thiết nghiên cứu đặc điểm gen hệ gen ty thể đơn vị mã hóa ribosome của sán phổi Paragonimus ……………………… CHƯƠNG ĐỐI TƯƠNG, VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 2.1 2.1.1 2.1.2 2.1.3 2.1.4 2.2 2.2.1 Đối tượng, mục tiêu, nội dung địa điểm nghiên cứu Đối tượng nghiên cứu ……………………………………………………… Mục tiêu nghiên cứu Nội dung nghiên cứu ……………………………………… ……………… Địa điểm thời gian nghiên cứu Vật liệu nghiên cứu ………………………………………………… Nguồn vật liệu mẫu sán phổi nghiên cứu 31 32 36 38 41 41 41 41 41 44 44 44 2.2.2 2.2.3 2.2.4 2.3 2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.3.6 2.3.7 2.3.8 2.4 2.4.1 Vật liệu khuôn DNA loại mồi sử dụng Hóa chất, sinh phẩm, dụng cụ Trang thiết bị Phương pháp nghiên cứu gen hệ gen ty thể (mtDNA) ……… 45 45 46 46 Phương pháp xác định cấu trúc sắp xếp gen hệ gen ty thể của sán phổi Paragonimus heterotremus P ohirai ………………………… 47 Phương pháp phân tích đặc điểm gen RNA ribosome ty thể (MRG)… 48 Phương pháp phân tích đặc điểm gen RNA vận chuyển ty thể (tRNA) 48 Phương pháp phân tích vùng không mã hóa hệ gen ty thể ………… 49 Phân tích phương thức sử dụng nucleotide tính toán giá trị độ lệch (skew/skewness) hệ gen ty thể ……………………………………… 49 Phương pháp tính khoảng cách di truyền giữa lồi sán phởi dựa chuỗi nucleotide của hệ gen ty thể …………………………………… 50 Phương pháp phân tích đặc điểm gen mã hóa protein sự “thiên vị” sử dụng mã ………………………………………………………………… 51 Phương pháp phân tích phả hệ dựa chỉ thị cộng hợp toàn 12 PCG của mtDNA …………………………………………………………… 52 Phương pháp nghiên cứu gen học đơn vị mã hóa ribosome (rTU) 53 Phương pháp xác định cấu trúc sắp xếp gen đơn vị mã hóa ribosome của Paragonimus heterotremus sán phổi châu A ……… 53 2.4.2 Phương pháp xác định độ dài, đặc điểm gen/vùng giao gen đơn vị mã hóa ribosome …………………………………………………………… 54 2.4.3 Phương pháp phân tích đặc điểm cấu trúc bậc hai gen 18S 28S rRNA của Paragonimus heterotremus P ohirai …………………… 54 2.4.4 Phương pháp phân tích đặc điểm cấu trúc bậc hai vùng giao gen (ITS-1 ITS-2) của Paragonimus heterotremus sán phổi châu A …… 55 2.4.5 Phương pháp phân tích phương thức sử dụng nucleotide giá trị độ lệch (“skew”) đơn vị mã hóa ribosome của sán phổi châu A 56 2.4.6 Phương pháp phân tích tương đồng nucleotide của gen 18S+28S rRNA phần mã hóa ribosome (rTU*) của sán phổi họ Paragonimidae 56 2.4.7 Phương pháp xác lập phả hệ dựa phân tích chuỗi gen 28S rRNA toàn phần (~3,8 kb) 56 2.4.8 Phương pháp xác lập phả hệ dựa phân tích cộng hợp ch̃i gen 18S+28S rRNA tồn phần (~5,8 kb) .57 CHƯƠNG KẾT QUẢ NGHIÊN CỨU ……………………………………… 59 3.1 Kết nghiên cứu đặc điểm hệ gen ty thể của sán phổi châu Á 3.1.1 3.1.2 3.1.3 3.1.4 3.1.5 3.1.6 3.1.7 3.1.8 Paragonimus heterotremus P ohirai họ Paragonimidae ……… 59 Cấu trúc sắp xếp hệ gen ty thể của sán phổi Paragonimus heterotremus (mẫu Việt Nam) P ohirai (mẫu Nhật Bản) 59 Phân tích đặc điểm cấu trúc gen RNA ribosome của ty thể (MRG) 65 Phân tích đặc điểm cấu trúc gen RNA vận chuyển ty thể (tRNA) .67 Phân tích vùng không mã hóa (NCR) hệ gen ty thể của P heterotremus P ohirai 69 Phương thức sử dụng nucleotide giá trị “độ lệch” (skew) hệ gen ty thể của P heterotremus, P ohirai sán phổi khác …… 70 Phân tích đặc điểm gen mã hóa protein sự “thiên vị” sử dụng mã hệ gen ty thể của P heterotremus, P ohirai sán phổi châu A 72 Khoảng cách di truyền giữa P heterotremus lồi sán phởi châu A dựa ch̃i nucleotide phần mã hóa của hệ gen ty thể …… 76 Phân tích phả hệ sán phổi họ Paragonimidae dựa dữ liệu amino acid cộng hợp của 12 PCG ……………………………… 77 3.2 Kết nghiên cứu đơn vị mã hóa ribosome của sán phổi họ Paragonimidae ……………………………………………………… 81 3.2.1 Cấu trúc sắp xếp gen đơn vị mã hóa ribosome của loài sán phổi Paragonimus châu A (P heterotremus, P ohirai, P iloktsuenensis, P miyazakii P westermani) ……………………… 81 3.2.2 Độ dài gen rRNA vùng giao gen đơn vị mã hóa ribosome của loài sán phổi Paragonimus châu A ……………………………………… 83 3.2.3 Đặc điểm cấu trúc gen rRNA (18S 28S rRNA) của loài P heterotremus P ohirai …………………………………………………… 87 3.2.4 Đặc điểm cấu trúc vùng giao gen (ITS-1 ITS-2) của loài P heterotremus P ohirai …………………………………………………… 89 3.2.5 Kết phân tích phương thức sử dụng nucleotide giá trị “độ lệch” (skew) đơn vị mã hóa ribosome của sán phổi châu A ……………… 92 3.2.6 Kết phân tích tương đồng nucleotide của gen rRNA phần mã hóa ribosome của sán phổi họ Paragonimidae …………………… 94 3.2.7 Phân tích phả hệ sán phổi họ Paragonimidae dựa dữ liệu đơn gen 28S rRNA toàn phần …………………………………………………… 95 3.2.8 Phân tích phả hệ sán phổi họ Paragonimidae dựa dữ liệu cộng hợp gen 18S+28S rRNA toàn phần ………………………………………… 98 CHƯƠNG BÀN LUẬN …………………………………………………… 101 4.1 Vê nghiên cứu gen/hệ gen ty thể của sán phổi Paragonimus 4.1.1 4.1.2 4.1.3 4.1.4 4.1.5 4.2 4.2.1 4.2.2 4.2.3 heterotremus Paragonimus ohirai ……………………………… Về thu nhận dữ liệu gen/hệ gen ty thể của Paragonimus heterotremus Paragonimus ohirai …………………………………………………… Về đặc điểm cấu trúc của gen hệ gen ty thể sán phổi Paragonimus heterotremus Paragonimus ohirai…………………… Về đặc điểm thành phần gen ty thể của sán phổi Paragonimus heterotremus Paragonimus ohirai……………………………………… Về ứng dụng mtDNA nghiên cứu phân loại phả hệ tiến hóa của sán phổi châu A ……………………………………………………… Về xây dựng sở dữ liệu mtDNA của họ Paragonimidae Vê nghiên cứu đơn vị mã hóa ribosome của sán phổi châu Á … Về thu nhận dữ liệu gen/vùng giao gen của đơn vị mã hóa ribosome của sán phổi châu A ……………………………………………………… Về đặc điểm cấu trúc của gen rRNA vùng giao gen đơn vị mã hóa ribosome của sán phổi châu A …………………………………………… Về đặc điểm thành phần gen của đơn vị mã hóa ribosome của sán phổi châu A …………………………………………………………………… 4.2.4 Về ứng dụng đơn vị mã hóa ribosome nghiên cứu phân tích phả hệ 4.2.5 Về xây dựng sở dữ liệu đơn vị mã hóa ribosome của họ Paragonimidae KẾT LUẬN VÀ KIẾN NGHỊ DANH SÁCH CƠNG TRÌNH CỦA TÁC GIẢ TÀI LIỆU THAM KHẢO PHỤ LỤC 103 103 106 109 112 114 118 118 120 122 123 124 127 129 130 DANH MỤC CÁC KÝ HIỆU, CÁC CHỮ VIẾT TẮT Chữ viết tắt Nội dung Giải nghĩa ATP Adenosine triphosphate Phân tử mang lượng bp Base pair Cặp bazơ DNA Deoxyribonucleic acid Axit Deoxyribonucleic ĐVLSN Zoonosis/zoonoses Động vật lây sang người et al Và người khác ETS External Transcribed Spacer Vùng ngoại gen “hairpin”/stem Secondary structure Cấu trúc nhánh “kẹp tóc” HTH Morphology Hình thái học IGS Non-transcribed intergenic spacer Vùng lề ITIS Integrated Taxonomic Information System Hệ thống thơng tin phân loại tích hợp ITS Internal Transcribed Spacer Vùng giao gen Kb (= Kbp) Kilo base pair Đơn vị đo (= 1.000 cặp bazơ) KCDT Genetic distance Khoảng cách di truyền KST Parasite Ký sinh trùng LNR Long non-coding region Vùng khơng mã hóa dài “loop” Secondary structure Cấu trúc “vòm” LsrDNA Large subunit rDNA Tiểu đơn vị lớn DNA ribosome LPCR Long- Polymerase chain reaction Phản ứng chuỗi trùng hợp dài LRU Long repeat unit Cấu trúc lặp dài MFE Mimum free energy Năng lượng kết nối tối thiểu Phet-LC-VN : TGTATATCATAGAATTAGTTATGATGTGGCACTTTGTTCTGAAGCGATGGGGCCGTTTTAGCTTTGTTGTGTCGGTTTAGGGTGTAAAATTTTTTGTAAGGGGGGGGTGTTGTGAAAAGTTTTTTTTTTGAGAATTTGCAGCTTTTTTCGGGTATACTTGAGATGGCTTTTGTTTTTTTT : 14760 * 14780 * 14800 * 14820 * 14840 * 14860 * 14880 * 14900 * 14920 * 14940 Phet-LC-VN : TTCTCCTTAATTTGGCTTTGTGTTTGAAAAAATTTTTACTTTTGAGTTGGGGATTTGTCGTGGCTTTTTTCGGGGAAGCTTGCGCGTTAAACTTTTGGCTTTTGGGGGGACTGGTAGTTGTGTGCTAGTATGTATATCATAGAATTAGTTATGATGTGGCACTTTGTTCTGAAGCGATGG : 14940 SRU1(114) SRU2(114) * 14960 * 14980 * 15000 * 15020 * 15040 * 15060 * 15080 * 15100 * 15120 Phet-LC-VN : GGCCGTTTTAGCTTTGTTGTGTCGGTGGGAAGAGAGATGGTTTATGCCAGGTAGTCTGCTTATCCCGAGCCTCTGTGTGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGTGTTGGTTTATGCCAGGTAGTCTGCTTATCCC : 15120 SRU3(114) * 15140 * 15160 * 15180 * 15200 * 15220 * 15240 * 15260 * 15280 * 15300 Phet-LC-VN : GAGCCTCTGTGTGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGTGTTGGTTTATGCCAGGTAGTCTGCTTATCCCGAGCCTCTGTGTGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGA : 15300 SRU4(114) SRU5(114) * 15320 * 15340 * 15360 * 15380 * 15400 * 15420 * 15440 * 15460 * 15480 Phet-LC-VN : GAAGAGAGATGGTTTGTGTTGGTTTATGCCAGGTAGTCTGCTTATCCCGAGCCTCTGTGTGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGTGTTGGTTTATGCCAGGTAGTCTGCTTATCCCGAGCCTCTGTGTGGGGGT : 15480 SRU5.9(111) * 15500 * 15520 * 15540 * 15560 * 15580 * 15600 * 15620 * 15640 * 15660 Phet-LC-VN : GCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGTGTTGGTTTATGCCAGGTAGTCTGCTTATCCCGAGCCTCTGTGTGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGGG : 15660 * 15680 * 15700 * 15720 * 15740 * 15760 * 15772 bp ======================================= Phet-LC-VN : GGGACTATAGGGGGGCCGTAGGAGGTATCTAACATATTTCCCCGCCGTGCTGTGTTTGAATCTTAGTGTGTTTGGTGGGCGAGTTGGGGCGTTCGAGTGATGTGGCTAGGAG : Hình PL3.2 Diễn giải trình tư gen vùng giao gen của hệ gen ty thể sán phổi Paragonimus ohirai, Nhật Bản (Pohi-Kino-JP) /GenBank: KX765277 (14.818 bp) cox3(645) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Pohi-Kino- : ATGAGATGATTACCTTTGTATAATTCGTGGTTGTTTTTTATGGCTCTTTTAAGGTTTTTTCTTTGAAAGATTGTAGGTGTTTTCTCTCTTTTTTGTTTAGTGGGACTTTCTGTTTTTATGTTGATTAGTGAGGTAATGCCTTATAAGGTTCATTATGAGCTTGGATTTTGGCTTTTTATT : M S W L P L Y N S W L F F M A L L S F F L W K I V G V F S L F C L V G L S V F M L I S E V M P Y K V H Y E L G F W L F I * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Pohi-Kino- : CTTAGGGAGGTTGCCATTTTTGGTACTTTGTTTGTAATGTGTCTTTGGAGGATGGATGGAGCGACGGAGTCGATTTCTAGGGCAACGGAGCTTCCACTTTTAGGATCATTTTTATTGATTGGTTCTTCAATTACGGCGACTACATATCATCATTGTCGGGGTTTGAATATTAGTTGGGTG : L S E V A I F G T L F V M C L W S M D G A T E S I S S A T E L P L L G S F L L I G S S I T A T T Y H H C R G L N I S W V * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Pohi-Kino- : TTTTTGGCGGTTACTATTGTTCTTGGGTTGAGTTTTATCCTATTGCAGGCTGTTGAGTTTTATGAATGTGAGTGTGATGTTTTAAAAGATGTGTATTATTCTAGTGTTTTTTCTACGGTGGGCTTGCATTTTAGTCATGTTTTTGTAGGGGTGTTGGGTTTATCTGTTTTAATGTTGTTG : F L A V T I V L G L S F I L L Q A V E F Y E C E C D V L N D V Y Y S S V F S T V G L H F S H V F V G V L G L S V L M L L trnH(His)(69) 180 360 540 cob(1119) * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Pohi-Kino- : GGGGCTAATACGGTTAAGCAGTGTTATGCTAAAGTTGCTGTGTGGTATTGGCATTTTGTTGATTATGTGTGGTTGTTTGTTTTTTTCTTGTTATATTTTTTGTAGATTTCCTCCCTAGGTTAATTTTTTTAAACCATTGGTTTGTGGGGCCGGGGATCTAATATTTTAGGGGCGGAAATG G A N T V K Q C Y A N V A V W Y W H F V D Y V W L F V F F L L Y F L * M * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Pohi-Kino- : TTATCTGTGTTGCGTTCTAAAGTTGTGGATTTACCTACAAATTTGTCTTTGAGATATCTTTGATGTGGAGGGTTTATGATCAGAAGGTTTTTAGTTCTTCAGTTAGGTTCTGGTGTGGTGTTGTCGTTGCTTTATGTGGCTGATGCTGGGTTGAGGTTTGGTTGTGTTTTAGATTTTACT L S V L R S N V V D L P T N L S L S Y L W C G G F M I S S F L V L Q L G S G V V L S L L Y V A D A G L S F G C V L D F T * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Pohi-Kino- : GGGGAGAGTGTTTTTGTTTGGCTAATTCGTTATCTTCATATTTGAGGGGTTACTTTTATTTTTATCTTGTTTTTTATGCATATGGGTCGGGCCTTGTATTATTCTAGTTATAGGAAGGTGGCGGTTTGAAATGTTGGGTTCATTTTATATCTTTTGATGATGATTGAGGCATTTTTAGGG G E S V F V W L I R Y L H I W G V T F I F I L F F M H M G R A L Y Y S S Y S K V A V W N V G F I L Y L L M M I E A F L G * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Pohi-Kino- : TATATATTGCCTTGACATCAGATGTCTTACTGGGCTGCGACGGTTTTAACGTCAATTCTTAATAGGGTTCCTTTAATTGGGCCCAGCTTGTACACTTTTATAGTTGGTGGATTTGGTGTAACAAATGCTACGTTGGTTCGGGTGTTTTCTGCGCATGTTTGTTTGGCTTTTATCATTGTG Y I L P W H Q M S Y W A A T V L T S I L N S V P L I G P S L Y T F I V G G F G V T N A T L V R V F S A H V C L A F I I V * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Pohi-Kino- : GGTTTTAGCATTATACATCTTTTTTACCTGCATAAGGGTGGTTCTAATAATCCATTGTTTGTGAGAGGGGGGTATAGTGATGTGGTTTTATTTCATAGATTTTTTACTAATAAGGACGGTTTAATGTTAATGATTCTTTTGAGATTACTTGGGGGATTTATGTTAATTTGTCCGGATTTG G F S I I H L F Y L H K G G S N N P L F V S G G Y S D V V L F H S F F T N K D G L M L M I L L S L L G G F M L I C P D L * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Pohi-Kino- : GTATTAGATGTTGAGAGATATTTGCAGGCGGATCCTTTGGTAACGCCCGTTTCAATAAAGCCGGAGTGATATTTTTTGGCTTTTTATGCTATGCTTCGTTCTATTGAGTCTAAGGTTGGGGGTTTAATTTTGGTGTTGGTTTTTTTATTTATTCTTTGGTTGCCGAGGTTTAATAATTCT V L D V E S Y L Q A D P L V T P V S I K P E W Y F L A F Y A M L R S I E S K V G G L I L V L V F L F I L W L P S F N N S * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Pohi-Kino- : TGTTGTTATTTTTTGGGTCGTCAGTACATTTTTTGAGGTGCTTCGTCGATGTTTTTTCTTCTTAGGTATTTAGGAGCTTGTCATCCTGAGGATCCATATATTTTGATTAGTAAGCTTTGTAGGTTGTTTATTGTTCTTTTTCTGTTTTTGTTTAAGGGATTGTGAACAGTGCCTTATTCT C C Y F L G R Q Y I F W G A S S M F F L L S Y L G A C H P E D P Y I L I S K L C S L F I V L F L F L F K G L W T V P Y S : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 nad4L(264) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Pohi-Kino- : TTTGGTGTTCCTTTTAGGGGAGTAAAGGCCTTTTAGTTAGTATGGGGGTGAGTGTTTTGTTTTTAGGTGGTGTAGTTGTGTTGTGTGGCCTTGTTTTATCATTGTCTCGTTTTTTAAATTGTTTAATTATCGTTGAAAAATTTAACGTGTTGTTGCTTTTTTCTTCGATATTGCAGCAAT : 1980 F G V P F S G V K A F * M G V S V L F L G G V V V L C G L V L S L S R F L N C L I I V E N F N V L L L F S S I L Q Q nad4(1260) * 2000 * 2020 * 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Pohi-Kino- : GACAGGAGAGACATATTTTGTTTATTGCCTTAATGGTAATTTTTACTGTTGAGGTTGTTTTGGGCTTAGTGGTGCTTACTCGATTATGAGACTTGGGAAGTTTGATTGGATTAGTTGGTTGATAGGTATTGGGATTGTTTTTTTTTTACATGTTGTTGTGGGTGGTGTTTGTCTTCCTCT W Q E S H I L F I A L M V I F T V E V V L G L V V L T R L W D L G S L I G L V G W * M S L G K F D W I S W L I G I G I V F F L H V V V G G V C L P L * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 * 2320 * 2340 Pohi-Kino- : TTTAGGGGGTTTGAGTGGACAGGTTTTTTGTTTTGATATCTTGAGCTTTTATCTGGGAGGCCTGTCCTTAATATTAGCGGTGTCTTTGGTTTTCTGACAGAGGTATTTTAGGGGGGTTTCTCGTTTTATGATTTTTTTGAGGTTAGTCTCTTCTTTGTTTAGGTATTGTTGTGTCCATTC L G G L S G Q V F C F D I L S F Y L G G L S L I L A V S L V F W Q S Y F S G V S R F M I F L S L V S S L F S Y C C V H S * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Pohi-Kino- : TTTAGGATTTTGGGTTTTTTACGAGGGTGCAATATTGCCTCTTTTGTTTTTATTAATTCGGGAGTCTCCTTATTCAGAGCGTTATGTGGCGGCGTGATATTTGCTGGGGTATGTAATTTTGACAAGGCTTCCAATGTTGTTGTGTTTATTCTATTTTTCTGTTGAGGCAGGTAGATTTAA L G F W V F Y E G A I L P L L F L L I R E S P Y S E R Y V A A W Y L L G Y V I L T S L P M L L C L F Y F S V E A G S F N * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 Pohi-Kino- : TGTACGTTATTGGGAGGAGGCTTCCGTAGGGGGCTTTAGGGTTATTTTATTGCTATCTGTGTTGTTTATAACTAAGATTCCTTTGCCTCCATTTCATGTTTGGTTGCCGATTGTTCATGCTGAGGCTAGAAGGCCGGTTTCTGTTTGTTTGAGAGGTTATATAATGAAGTTGGGGTTACT V R Y W E E A S V G G F S V I L L L S V L F I T K I P L P P F H V W L P I V H A E A S S P V S V C L S G Y I M K L G L L * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Pohi-Kino- : AGGTGTTTATCGTTTCTGTTTTTGATTACTTCCGAGGTATATCTTTTCTGTGCCTTATGTAGGTATTGGGGTTTTGATGACGTTGCTATTTTTCTTTAGTGCAGCTCGAGAATTGGATGGGAAGCGTTGATTGGCGTTTATGAGATTAGCCCATATTTTGATTCCTGCAGTGTGTCTTTG G V Y R F C F W L L P S Y I F S V P Y V G I G V L M T L L F F F S A A R E L D G K R W L A F M S L A H I L I P A V C L C * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Pohi-Kino- : TGTTAGTGGCTTTGATAATTTAGGTATTTCCTTTTTGTATTGTTTAGGGCATGGTGCTTCAGCTGGTGTTATGTTTCTTTTTTTGTGGCTTGCCTACGAGGTATCTGGTAGTCGTAATTGAGCTGTTTTGAAGTTTGGTCTTTCTAATAGTTTGTTGATGCGCTTCTTAGTTGGAGCATG V S G F D N L G I S F L Y C L G H G A S A G V M F L F L W L A Y E V S G S R N W A V L K F G L S N S L L M R F L V G A C * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Pohi-Kino- : TCTTTGCACTGTCGCTTCTTTACCTCCGACGGTACAGTTTTTTTGTGAGGTTCATACTTTGTGGGAGGCTGGGCCACTTGCTTTTAAATTTGTTTTTGGTCTTTTTTGCTATCTCTTTTGTGGTGGTCTGGTACCGGTTTTTACGTTGGGTGGGTTGTTAACTCGTCATTATAGTATTGG L C T V A S L P P T V Q F F C E V H T L W E A G P L A F N F V F G L F C Y L F C G G L V P V F T L G G L L T R H Y S I G trnQ(Gln)(62) : 2160 : 2340 : 2520 : 2700 : 2880 : 3060 : 3240 trnF(Phe)(66) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 Pohi-Kino- : GTTTAGTGTAAGGCCTATTTTTATGGGATTGTTTTCTGTGTTGTTCTTATTATTTTGAAGGTTTACTTTATTTTTAGTTGGGTAGCTTTATTTATGTGCAGCTCAGTGTTTGTTGCATTTTATGTTTTGGTCATAGAGGGGTTTGTTACTAGCTGCAGTTTAGACTCTCTTCTAGCTTAT : 3420 F S V S P I F M G L F S V L F L L F W S F T L F L V G * tnrM(Met)(65) atp6(516) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Pohi-Kino- : TTGAAAGCATTAGTTTGAAGAGCTGGGGAAATAATTATTTATGGGAGAGGGGGGAGGTAAGTTAAGTTAATAAACTGTATGGCTCATGCCTATGTAATACATTTTGTCCTCCCCTATGTATGTGACTCGTTTAGGAAGCATTGGGAAGTTTTTAGTGGGCTTAATATCTGGTGGACAAGG : 3600 M Y V T R L G S I G K F L V G L I S G G Q G * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Pohi-Kino- : CGATTTTATTTATCGTGTTGTGTTGTGTGGTTTGCTTTTTTGTTTTTTAAGTCTTCGGTTTCCTTATCTCTTTGGTTTAAGAGGATTTGGCTTGTTTTTGTTTTTTGTGATTTCTCCCTTATTCTTATCTTTGTTCTTCTCTCGATTTAGAAATGGGGGAGGTCTCCTTTTTTTTTCTTG : 3780 D F I Y R V V L C G L L F C F L S L R F P Y L F G L S G F G L F L F F V I S P L F L S L F F S R F S N G G G L L F F S C * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Pohi-Kino- : TTTCGTACCTGAGGGGACTCCTCTTTGAATAGCCCCCTTCGTTTGTATGGCGGAGACTTTAAGTTATTTGGTTCGTCCTGTAGTTTTAATGATTCGTCCCTTTGTTAATTTGTCAATGGGCGCCATGGGAGGATTTGTTTTGGGTTTGCTTAGGTTTCGTTTTGAGTGGGTGGTTTTTTT : 3960 F V P E G T P L W I A P F V C M A E T L S Y L V R P V V L M I R P F V N L S M G A M G G F V L G L L S F R F E W V V F F nad2(867) * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Pohi-Kino- : TTCGTTTTTCCTCTTTTTTTATGAGGTCTTTGTTGCGGTTGTGCATTGATTTATAGTTTGTAACATTTTGTCTTTTTCTGAGAGTCATTAGTTATGTGGGGATATTTGTTAGGAATTGTTAGTTTGTTAGGGGTTTTTATTTTTTCGTTTTGTTTATTTTTAAGGGATGGTTTGGCAATG S F F L F F Y E V F V A V V H W F I V C N I L S F S E S H * M W G Y L L G I V S L L G V F I F S F C L F L S D G L A M * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Pohi-Kino- : TTTTGATTGTTTTTAGAGCTAGGTGCTTTGTCTTTAATTCCTTCATTTTTTTTGCGTTTAGGGGATGGTGTTTTGGAAGCTTTGTTTAATTATTTAATCGTTTCTAGGGTTTCCTCTTCCTTAATGATATGTGGGTTTTTGTATGAGAGTTTGCTTTTTCTCTGTTTTTTAGGTTTGTTG F W L F L E L G A L S L I P S F F L R L G D G V L E A L F N Y L I V S S V S S S L M I C G F L Y E S L L F L C F L G L L * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Pohi-Kino- : GTAAAGTTTGGTGTGTTTCCGTTTCAAGGTTGGATTTATAAGGTTTTATTGAGATCTGGGTGGGTTGTCGTGTGGGGATTTTCTGTTTTTTTGAAGCTTCCTTTTTTATTTATGTGCTTTTTTTTGTGTAGTGGTGGGAGATTCTTTTTGAGGGTATCTTGCCTTTTTTCTTTTCTTTTT V K F G V F P F Q G W I Y K V L L S S G W V V V W G F S V F L K L P F L F M C F F L C S G G S F F L S V S C L F S F L F * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Pohi-Kino- : TTAGGAGGGTTGTTTTGGTGTTATAGTTATAGTTGATGTCATTGTTGATGCCATATGATGCTTTCTTCTAGTGCTGTGTTAGTGGCTATGTCTGTTAGGGGTTCTGCAGAGGCCCTTTTTTGTCTTTTTGTTATTTATGGTGTTTGAAGGAGTATGGTGATTTTATTTTTCTCCCATATT L G G L F W C Y S Y S W C H C W C H M M L S S S A V L V A M S V S G S A E A L F C L F V I Y G V W S S M V I L F F S H I * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Pohi-Kino- : GAGAAGATGGGCTTGGTGGAGGTTGGGTCATATTTTTTGTTTTTGATTTTGTTGGTCTCTTTACCGATTTCTGTTTCTATTTTTTATAAGTTATGGATGGCGGTTAGGATTTATTTTTGTTATTTTCCGGTTTTTGTTGCTTGGTGCTTGTATAGAATATCAGAGCAGTTGTTTTTGGTC E K M G L V E V G S Y F L F L I L L V S L P I S V S I F Y K L W M A V S I Y F C Y F P V F V A W C L Y S I S E Q L F L V trnV(Val)(64) : 4140 : 4320 : 4500 : 4680 : 4860 trnA(Ala)(66) * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Pohi-Kino- : AGGTATCTTGTTAAGGATAGTGTGTCATGTGACACATATGGCGGAGTCTTTTTAAGCTAGTTTAGTTGACGAGATAGTTTAATGAAAATGTCTATTTTACACGTAGAGGTTGGTTATGGTGCCTATCGTCAGGGATGACATAGTTTAAGTTAGGAGATGGCTGCTCTGCAAGTAGCCGGT : 5040 S Y L V K D S V S C D T Y G G V F L S * trnD(Asp)(67) nad1(906) * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Pohi-Kino- : GAGGGTTCCTCTGTTGTTGGGGGGGACAGCCTTAGTTTAATTGAAGGATGATAGTTTGTCGTGCTATTGGTGGGTTTGATTCTCAGGCTGTGATGGTTTCTTTGTTGACGGGGGTTTATTTATTTTTAAGGAGGCTTATTTCTTTTGGTTTAATTATGCTGTTTGTGGCCTTTTTTATTT M V S L L T G V Y L F L S S L I S F G L I M L F V A F F I * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Pohi-Kino- : TGGGAGAGCGTAAGGTGTTAGGGTATATTCAGTTTCGTAAGGGTCCTAATAAGGTAGGTTTGGTGGGATTACTACAGAGGTTTGCAGATCTTTTGAAGTTGGTTATAAAGTTTAAGGTTTCTTCCTTTCAGGGTCGTAGTTGGCTTTCTTGATGAGGGGTGGGTGTCTTGGTTCTTTTGG L G E R K V L G Y I Q F R K G P N K V G L V G L L Q S F A D L L K L V I K F K V S S F Q G R S W L S W W G V G V L V L L * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Pohi-Kino- : GTTGTCTTTATTGTGTCTTTTTCTCTTTTAGTTATTCTGGTTTGAGGAGGAGAAATAGAGCTCTTTGGTTGTTGGTCATTACTAGTGTTACAGGGTATAGTCTTTTAAGGATAGGTTGGGGTTCTTATAAAAAGTATGCTTTATTGAGATGTTTGCGATCTGCGTTTGGTTCTGTGACTT G C L Y C V F F S F S Y S G L S S S N S A L W L L V I T S V T G Y S L L S I G W G S Y N K Y A L L S C L R S A F G S V T * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Pohi-Kino- : TTGAAGCCTGTTTTATGTGTATTGTTTTATTGCTGGCTATAGTTAGGGGGATTTATTCATTAGGTCCTTGTTTGGAGCGTTCTTTTTTTATTTCTTTATCTTTGCCCTGTTGTTATATTCTTTGATTGGTGGGTAGTTTGTGTGAGTGTAATCGGACTCCTTTGGACTATGCTGAGGCTG F E A C F M C I V L L L A I V S G I Y S L G P C L E R S F F I S L S L P C C Y I L W L V G S L C E C N R T P L D Y A E A * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Pohi-Kino- : AAAGGGAGCTTGTTAGGGGGTTGAATACGGAGTATTGTAATGTGCCGTTTACTTGCCTTTTTGCTTGTGAGTACTTAATAATGTTTATTTTCTCTTGGTTAGGGAGGGTAATATTTTGAGGAGGTGGAGCTGTATTATTTTTGACTGTTTTTCACGTTATTTTTTTTGTGTGGGCCCGGG E S E L V S G L N T E Y C N V P F T C L F A C E Y L I M F I F S W L G S V I F W G G G A V L F L T V F H V I F F V W A R : 5220 : 5400 : 5580 : 5760 : 5940 trnN(Asn)(70) * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Pohi-Kino- : CGACGTTGCCTCGAGTTCGTTATGATTATTTTGTTCGGTTTATGTGAAAGTGGGCTGTGTTGGTTTTAGTTTTTTCTTTTTTTTTGGTTTTGGTTTAGGGGTGAATTTGGGTCGTGTAGATTATTTTTAAATCCTAAGGCTGTTAACCTTAAGAAGAGTTTTATTCGGTTCCGCGGCCAA : 6120 A T L P R V R Y D Y F V R F M W K W A V L V L V F S F F L V L V * trnP(Pro)(67) rnI(Ile)(63) trnK(Lys)(64) * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Pohi-Kino- : CACGGTGGTCTAAAATTTTAGGATATGAGTTTTGGGGACTCGTGGTCCCGCTAGGGCTTGTTGATTTTTTGCCGGTTGGGCTGCGTTAGCAGGTTGCTGTGATATAGCGGTTGTGAGATTTTATCTTCGTCGGTAAGCAGGTATTCTAAAAGTAGCTTATTTTAAAGCTTTGGATTCTTA : 6300 nad3(357) * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Pohi-Kino- : CTCCTAGGATGTTGCTTAAACCTTTTAGGATGGGAGTTTTGTTATGTGGTGCTGGATTAGGAGGTTTGATTCTTTTGCTGGTTTGCCTATTCCATAGTTTTGTTTGAAATATTAGGGATAGTTTTGGGTTAGGTTTACGTTCTTGGGTAAGTTCTTTCGAATGTGGTTTTGTTTCTCAGC : 6480 M G V L L C G A G L G G L I L L L V C L F H S F V W N I S D S F G L G L R S W V S S F E C G F V S Q * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Pohi-Kino- : GTGTTGTTGAGGATTATTTTAGGCATACTTATTTTATTTTGGTGGTTTTTTTTGTTGTTTTTGATTTGGAGATTTCTTTGTTGTTGAAAATGCCGTTGCAGGGTGTTTTGTATAAGAATTTGGGATTTTATGTAGTTTTTCTTCTTTTGTTGGCGTTCGGATTTGGTGCAGAAATTTATA : 6660 R V V E D Y F S H T Y F I L V V F F V V F D L E I S L L L N M P L Q G V L Y K N L G F Y V V F L L L L A F G F G A E I Y trnS1-AGN(Ser1)(62) trnW(Trp)(65) cox1(1533) * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Pohi-Kino- : AGGGTTATGCGGAGTGAGGTTATTAGATTGATTGGGGGCTTGTTTGGGTTGTCGCTGCTAACGAGAATTGGGGTATTAATAATATCCGGCCCCCTTTTCTGGAATAAGCTAGGTTATTGTTTGACTATCTGTTTTCAAAACAGAGGGAGGCCTTGGGCCGTTTATTGATTATGACTTGAT K G Y A E W G Y * M T W * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Pohi-Kino- : TATTTACTTTGGATCATAAGCGTATTGGTATGATTTACATGGTCTTGGGTGTGTGAGGTGGATTTATTGGACTTTCTTTAAGAATGTTAATTCGTTTGAATTATCTTGATCCGTATTATAATATTGTCTCGTCGGAGGTTTACAATTATGTGATAACTAATCATGGGGTTGCTATGATTT L F T L D H K R I G M I Y M V L G V W G G F I G L S L S M L I R L N Y L D P Y Y N I V S S E V Y N Y V I T N H G V A M I * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Pohi-Kino- : TCTTTTTTTTGATGCCTGTGTTGATAGGAGGATTCGGTAATTATCTTCTTCCTCTTTTATTGGGTATACCTGATTTGAATCTTCCTCGTTTGAAAGCTCTTAGGGCTTGATTAATGCTTCCTTCTGCTGTTTGTCTTAGTTTGAGCATGCTAGGAGGGGCAGGAGTAGGATGAACCTTCT F F F L M P V L I G G F G N Y L L P L L L G I P D L N L P R L N A L S A W L M L P S A V C L S L S M L G G A G V G W T F * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 Pohi-Kino- : ATCCGCCTTTATCAGGTGGGGATTATTCTGGGTGAGGTACAGATTTTTTGATGTTTTCTCTTCATTTGGCGGGGTTATCTAGTCTTTTGGGTTCCTTAAATTTTATTTGTACTATTGTTAGGGCGTTATGCGAGCATACGGTGGGTCGTAGGTCTATAATTGTTTGGGCGTATCTTTTTA Y P P L S G G D Y S G W G T D F L M F S L H L A G L S S L L G S L N F I C T I V S A L C E H T V G R S S I I V W A Y L F * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 Pohi-Kino- : CGTCTGTTTTGTTACTACTTTCTTTACCAGTATTAGCGGCTGCAATTACCATGCTTTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCCTTGGGTGGAGGTGATCCTGTTTTGTTCCAGCATTTGTTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTAATTTTACCAGGAT T S V L L L L S L P V L A A A I T M L L F D R N F S S A F F D P L G G G D P V L F Q H L F W F F G H P E V Y V L I L P G * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * 7740 Pohi-Kino- : TTGGGATTGTGAGACATATTTGTATGACTCTAACTAATAAAGATTCGTTGTTTGGTTATTATGGATTGGTGTTTGCTATGGGGGCGATTGTGTGTTTGGGAAGTGTTGTCTGGGCCCACCATATGTTTATGGTTGGTTTGGATGTTAAGACTGCAGTATTTTTCAGATCTGTTACGGGGG F G I V S H I C M T L T N N D S L F G Y Y G L V F A M G A I V C L G S V V W A H H M F M V G L D V K T A V F F S S V T G * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * 7920 Pohi-Kino- : TGATAGGTATTCCTACAGGGATTAAGGTTTTTTCTTGGTTATTTATGTTAGGGGGTACTCGTCTACGGTTTTGGGATCCTGTAATATGATGAATTCTTGGGTTTATCTTTTTGTTTACAATAGGGGGTGTAACTGGAATTATCTTGTCTTCTTCTATTTTGGACAGTTTATTGCATGACA V I G I P T G I K V F S W L F M L G G T R L R F W D P V I W W I L G F I F L F T I G G V T G I I L S S S I L D S L L H D * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * 8100 Pohi-Kino- : CTTGGTTTGTTGTTGCACATTTCCATTATGTTTTATCCCTGGGATCTTATAGCACTGTTGTAATATCCCTTCTGTGATGGTGGCCTATTGTGACCGGGTTTAGTTTAAATAAGTATTTGTTGCAAGGGCATTGGGTGGCATCAATGGTAGGGTTTAATTTTTGTTTTTTCCCCATGCATT T W F V V A H F H Y V L S L G S Y S T V V I S L L W W W P I V T G F S L N K Y L L Q G H W V A S M V G F N F C F F P M H * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * 8280 Pohi-Kino- : ATCTTGGGGCTTATGGTCTTCCACGTCGGGTGTGTAACTATGAGCCGGCCTTTCAGTGATTAAACAATATTTCTTCTGTTGGAGCTTTAATTTCTGTGGTTAGGGCTTTTTTTTTGGTTTTTATTCTTTGAGAGTCTCTTGTGGTTGGAAATCGTGTTGTTGGGGTTTGAGGTAGCAAAT Y L G A Y G L P R R V C N Y E P A F Q W L N N I S S V G A L I S V V S A F F L V F I L W E S L V V G N R V V G V W G S N trnT(Thr)(66) Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : Pohi-Kino- : : 7200 : 7380 : 7560 : 7740 : 7920 : 8100 : 8280 rrnL(974) * 8300 * 8320 * 8340 * 8360 * 8380 * 8400 * 8420 * 8440 * 8460 Pohi-Kino- : CTTTGGTTATGAATGCTTTGGTTACTCCTGTGCCTCATCATAGGGTTTACATTAGTGGGCCGTCTCGTTGGCTTTCTTTTTAGGAAGTGTAGTTTATGAGAGAATGCTGCTTTTGTAAAGCAGTGGTGTAATTTGATTAGCTTCTTTGATTTTGAGATCGTTTTAGTTTTTGTGTTTAGG S L V M N A L V T P V P H H S V Y I S G P S R W L S F * * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 * 8640 Pohi-Kino- : GGGTGTCGTACCTTTTGCATCATGATTCTTTGACGGAAGTTGGTTATATTCAGTGTCTCGAAAGGCAACGATTTAGGTTTATCTTGTTTTAGAACTTAGGGATTGGCGGGTAGCTGAATCTTAAAGATAGACTTGGAGGCGATAGGTAATGCGTGTTGCTTTATAGCTAGTTTTTGAGTG * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 * 8820 Pohi-Kino- : CTTTTTCGTTTTAGCCTTGTTGCTTAAATGTGATGGGGCAGTTAAGAGAGTGTATGTCTATGGTTTTAAGGTGGGTTAAAGGTACCCAGCTTTAGTTTCGTGTTATAACGATTTTTTGTCATAGGTTTTCTTTAGTTTGGTACTCAAATAGTTTGGAATATTTTGTTTAAGTATGGAGGG * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 * 9000 Pohi-Kino- : ATTAGTAGTTTTAATAGTTCCTTTTTTTCTCGAATTTCTTCAGTCTGTTTCTTAAAAACATTGCCATTGTTAGAATTTTGATGGTAGGGCCTGCTCGATGTTTTAATAAATGGCCGCAGTATTTTGACTGTGCTAAGGTAGCATAATTAGTTGCCTTATAATTGAAGGATTGTTTGAATG * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 * 9180 Pohi-Kino- : GTTTAACTAGAGGAGTATTTTAGATGGGGGCTATTATTGAAATTAGGTGGCTGGTGCAGATCCCAGCGTTTTTTAATTAGACGGAAAGACCCCGAGAGCTTAATAATAATTTGTTGTTTACTTGGGGCAAGAGCATATGCTTTATATGTTTTTGGATCCTTTTGGAAGGAGATTAGTTAC * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 * 9360 Pohi-Kino- : CTCGGGGATAACTAGGTAAGAGGCAAGGAGAGGTCATATCGATTTGCCTGTTTGCTATCTCGATGTTGACTTAGGGTGAGTATGAGGGTGCAGTAGTTTTCATACATGGTCTGTTCGACCGTAGTTCCCTTCATGAGTTGAGTTAAGACCGGCGTGAGCCAGGTCGGTTCTTATCTTTTA trnC(Cys)(66) : 6840 : 7020 : 8460 : 8640 : 8820 : 9000 : 9180 : 9360 rrnS(736) * 9380 * 9400 * 9420 * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 TTGTTGCGGTTTCGTACGAAAGGAGCATCCGTGATTTAGGTTGGGCGAGTTATGTGTTTAACATAGTTTACTCTGCAAAGGTGAATTTGATTTTTTGGTCCTCGTCCTAGTTCAATTTAATTCTGGTTGTGGAAGGGGTCAAAGTAGTACTACATAGGCTTTGTTTCTTTCAGGAAGTTG * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 ATAGGTTTGTGGCGTTGCTTAGTGGTTGGATCTTGGTTTTTGAGGTAACTCTGATTGGGCTGCTTTGAGGGAGGTGGAAAATTCACAGTGCCAGCATCCGCGGTTATTCTGTTAATCTCCTCTTCTAGCTGGTTTAAATGATTTGCAGGAGATTTCGGACTTTTGGGTAGAATTGTTGTC * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 TTGTTGAGAAACACTTGCATTTTTTTGGAGAACTCTCTAGGAAAAGGATTAGAGACCCTTGTACTGAGTGGAGTATTTTGTGGCCATGGTGTAAACTAAAAGGATTTGGCAGCCGATGATGTTCCTCCGGGGGAGTGTGTGGCGTAAGAGATAGTCCACTTAGGATCTTACCATCATTAT * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 TAGTTAGTGTATATCCGGTTTAAGATTCGTGATTTGTGTCATTGAGTGTTATTGATTTAATTGAATCCAGGTCAATGTGCTGCTTATGTGATGGTGCTTGTGCACTACTTTTTGAATATTCTTCAATGTAAATTGTGGGAGGTTTAGGACTCGGAAGTAGGCGGATTGAGGTTTGTTCTG cox2(600) * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 * 10240 * 10260 TCGAATTTGGATTTAGTTGGGGTACATACCGCCCGTCACCCAGGGTGATAACTGTGGTAAGTCGTAACATGGTAATGTTGGGGGAACCGGACATTAGTTGGTAAATTCTTAGTTAGGAGTACCTATGTATGTGTATACACCATTATATTCTGAGATGGTAGGTTATGTTTCTTTTTTGTC M Y V Y T P L Y S E M V G Y V S F L S * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 AGCTTTTATACCTATTTGGGTTTTTTTAGTTTTGGGATGACAGCTATGTAGATCTTATGGAACTGCAGCACTTCGTAAAGAAAGAGATTTGTTGGAGTTTTTTTGGACGGTGGTTCCCAGAGTTTGTGTTGGATTTCTTTGTCTTTTAAAATTGGGTTGTTTGGGTAAAGATTTGGTTTT A F I P I W V F L V L G W Q L C S S Y G T A A L R N E S D L L E F F W T V V P S V C V G F L C L L N L G C L G N D L V L * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 GAAGACGCGTGGTTTGATTAAGGTGATTGGTCGACAATGATATTGAAGTTATGACTTAAATGATGGCTGTGGGTTGTATGATTCTATGATGAGGGATTTTATTGATGGTGTTGATAAGCCATTGCGTGTTTATGTGGATACTCCTTATAATCTTTCGGTGACATCTAGGGACGTTATTCA K T R G L I K V I G R Q W Y W S Y D L N D G C G L Y D S M M S D F I D G V D K P L R V Y V D T P Y N L S V T S S D V I H * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 TTCTTTCGCTCTTCCTATTGTTAATTTGAAGGTGGATGCCATACCTGGGCGGTTAAACAAAGTCTTTTTTTGTATGAATCATGTTGGTGTTTTTGTCGGATATTGTAGGGAGTTATGTGGTGCTGGACATGCGTATATGCCTATAGTGATGGAGGTTATTGAGAAGGCCTTTGTTAAGGA S F A L P I V N L K V D A I P G R L N N V F F C M N H V G V F V G Y C S E L C G A G H A Y M P I V M E V I E K A F V K D : 9540 : 9720 : 9900 : 10080 : 10260 : 10440 : 10620 : 10800 nad6(453) * 10820 * 10840 * 10860 * 10880 * 10900 * 10920 * 10940 * 10960 * 10980 Pohi-Kino- : TTAGTGAGTATTGTATTTTTAAGGCTGTACTTTTCAAGGTTGATTAGTTTTTCTTTTATTAGGCATCCTGTAATTTATTGTGTTCTTCTTTTGGTTAGTTCTTTAAGAATTAGTGGGTACTTGTATTCTGTGTTGGGCTTGTCTTGGTATTTGGCGTTATTTTGCTTGGTTTATGTAGGG : 10980 * V S I V F L S L Y F S S L I S F S F I S H P V I Y C V L L L V S S L S I S G Y L Y S V L G L S W Y L A L F C L V Y V G * 11000 * 11020 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 Pohi-Kino- : GGGATTTATGTTTTATTTATTTTTGTTTCTGTACATATCTCTAATCCGATGTCTATTGGTGGCGGAAGGGGCCTTGTATTTTTGATAGCTTTTGGAAGGTTTATGTTAATTTTCAGTGGGGGCCTAGGTCATAGGCTTGGAGGGTTTTGTGAGGAGAGGCACTATCTGTGCTCTTATTTT : 11160 G I Y V L F I F V S V H I S N P M S I G G G S G L V F L I A F G S F M L I F S G G L G H S L G G F C E E S H Y L C S Y F trnY(Tyr)(61) * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 * 11300 * 11320 * 11340 Pohi-Kino- : GAGGGGTTCTCGTATTGTTTGTTTTGTTTGGTCTTAATGATTGGTTTTGTCGGTGTTAGGGTTATTTCTGGAGAGAAGGACTCGTTTTTTCGTTAGATTACCGACTTAGTATAATAAGTGCGTGAGATTGTAAATCTTGAAGAGGGTTTTCCAGTCGGAGAGAATTCTTTTTTAAGAGTG : 11340 E G F S Y C L F C L V L M I G F V G V S V I S G E K D S F F R * trnL1-CUN(Leu1)(65) trnS2-UCN(Ser2)(64) trnL2-UUR(Leu2)(63) * 11360 * 11380 * 11400 * 11420 * 11440 * 11460 * 11480 * 11500 * 11520 Pohi-Kino- : CCAGACAAAATGGGTTTGATTTAGGATTAAATAATGGCTGCTTATGCCCTCTTGCGGGCAAGGATTTTCCGGAAGGAATTTGAAATTCTTTCTTTCATTTAGTTAATGATGCCTGCTATCAGAGTTTAAGGATGAACATGGGTGCCAGATAAAATGGGTTGGTTTTAAGCATCAAATATG : 11520 trnR(Arg)(69) nad5(1584) * 11540 * 11560 * 11580 * 11600 * 11620 * 11640 * 11660 * 11680 * 11700 Pohi-Kino- : AGGGTTTCTCCCCGTGTAGTTATGCGTGATCTGATATCCCGGTGTAGTGGGGATTACGTTTCGGCCGTAATTGGGGGTGGTATAACATTCTATCAGAGGTGTTGGCGTTAAGGGTGGTTTTGGTTCTTGGCAGGTTGGTTTTTTGAAGAATTGGGTTAGTTGGTTTGGGGGCTTCTTTAA V L A L S V V L V L G S L V F W S I G L V G L G A S L * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 * 11840 * 11860 * 11880 Pohi-Kino- : AAGTTTGAAATTATGCTTGAAAGGATGGTGTGGAGTTGTTGATGTTAGACGAGGTCAGTTTAATTTGTAGCTTTATGCTTTTCTGTTGTGGAAGATTAGCACTTTTGTATTGTTATCATTATTTCGCTGATTCCGGAGAAGGTGTCTTATTGTTTCCGTTAATAGTTTGGTTTTTGGGGG N V W N Y A W K D G V E L L M L D E V S L I C S F M L F C C G S L A L L Y C Y H Y F A D S G E G V L L F P L I V W F L G * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 * 12020 * 12040 * 12060 Pohi-Kino- : TGATGGGCATTTTAATTTTTAGGAGGAGTCTTATTTTTTCTCTAGTTTTATGAGAATATTTGGGGTTGGTGAGGTTTCTACTAATTTTGTTTTATTCTAACAGGAGTAGAGTCCGGGCTTCCTTAATAACTTTATTCGCGTCTCGATTTGGAGATGTGTCTTTGTTTATGCTTATTCTTT V M G I L I F S S S L I F S L V L W E Y L G L V S F L L I L F Y S N S S S V R A S L I T L F A S R F G D V S L F M L I L * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 * 12200 * 12220 * 12240 Pohi-Kino- : GATGCGGTGTCTGGTGAGGGATAGTGTTGGCCTCCTTTTTTTCTTTTTTTTTTCTCCTTGTTGTTTTAAGTAAGAGAGCTGGTTATCCTTTTATTTCTTGGCTTTTGGAAGCTATGCGTGCTCCTACTCCTGTGAGTTCTCTTGTACATTCTTCTACTTTGGTTGCTGCAGGGGTTTGAT W C G V W W G I V L A S F F S F F F L L V V L S K S A G Y P F I S W L L E A M R A P T P V S S L V H S S T L V A A G V W * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 * 12380 * 12400 * 12420 Pohi-Kino- : TTCTTTTACGTTATCAGAGGCTTTTGGTTTCTTTTTTGAGGGGGTGATTGTGTTTGTTTTGTGTTTTGACTATTATTTTAAGGGGTATGTGTGCTTCTTTTTTTAAAGATTTGAAGAAGGTTGTTGCTTTATCGACTTGTAATAATATTTCTTGGTGTGTGGTTTTTTTTGTCTGTGGGG F L L R Y Q S L L V S F L S G W L C L F C V L T I I L S G M C A S F F N D L K K V V A L S T C N N I S W C V V F F V C G * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 * 12560 * 12580 * 12600 Pohi-Kino- : ATTTGTTTCTTTGTTTGTTACAGCTTTTGACGCATGGTGTTTGTAAGTGTTACTTGTTTATGTCTGTTGGTGATTTAATGTCACAGTCTGGTGGAAGTCAGAGTTCTGTTGGAGTATATGGTTCTCGTTATGTTGGTGTGTTGGGTCCCATTTTGCAGAGTGTTTTGGTTTTTTCTTTGT D L F L C L L Q L L T H G V C K C Y L F M S V G D L M S Q S G G S Q S S V G V Y G S R Y V G V L G P I L Q S V L V F S L * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 * 12740 * 12760 * 12780 Pohi-Kino- : CTGGTCTTCCGTTTATGGGTGTTTTTTTTAGGAAGCATGGTCTTTTTTCGGCTGTTTCCTATTGTTATGGGTTGGGATTTTTGTTGGTGTTTTGATTTGCATTTTTTTTGACATATGTTTATTCTGTGCGTTTGAGGCTACTTTTATTAAAGGTTTCTAGAGGTTTGTCGAGTGGTTATT S G L P F M G V F F S K H G L F S A V S Y C Y G L G F L L V F W F A F F L T Y V Y S V R L S L L L L K V S S G L S S G Y * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 * 12920 * 12940 * 12960 Pohi-Kino- : TCAGTGCCTTTCTTCCTATAGCAGGTTTATGTTTGTTGGGTACGTTTTTAAATTGGTTTGGGTTGATTTTGTTTGATGAGAGTTTTAGACTGGGTATTTGGTGGGGTAGTATGATGTTGTTGGTTCAAGCTTTAGGGTGTTTGATGGGGTGGTTTTTGTTTTTGGGTGTGTTTAGATCTG F S A F L P I A G L C L L G T F L N W F G L I L F D E S F S L G I W W G S M M L L V Q A L G C L M G W F L F L G V F S S * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 * 13100 * 13120 * 13140 Pohi-Kino- : GTGTTTGGGTTGTTTGAAATTCTTTATTATGGGGTAGGGATTGTTTGGTAGGGTTTATTTATGATGGGTTTTTGAAGTTAACAGAGGTAGCGGTTCTTTCTTTTTATCGGTGGGAGCTTTATAGTTTGGCACTTTTTAAGGCTGGTGGTGATAATTCATTTATAGGAGTTTTTACCTCTT G V W V V W N S L L W G S D C L V G F I Y D G F L K L T E V A V L S F Y R W E L Y S L A L F K A G G D N S F I G V F T S trnG(Gly)(72) : 11700 : 11880 : 12060 : 12240 : 12420 : 12600 : 12780 : 12960 : 13140 LRU1(292) * 13160 * 13180 * 13200 * 13220 * 13240 * 13260 * 13280 * 13300 * 13320 Pohi-Kino- : TGAAATTATTGGTGTTGGGTAGCGTTTTTGTGATTTTTTTGTTTTCTCTCTTTTTTTGTTAAGTGGTGGTTTATGCTAGTAGTATATTTTGAGTGAAGTATTCTGTCTTTCCAAGTCAGAGGGCCATGTGTTTTGGCTAGCATAGTATTATGATTTCTAAGTCTGTTTGGGTTTAGAGGG : 13320 L N L L V L G S V F V I F L F S L F F C * * 13340 * 13360 * 13380 * 13400 * 13420 * trnE(Glu)(69) 13440 * 13460 * LRU2(292) 13480 * 13500 : 13500 Pohi-Kino- : AGTTTAAAAAATTATCCGATGTTCGTTGTTAAATAATTCGTAAGGGGGGGGTATTGTGAAAAAAAAATTTTTTGTAAATTTGTAGCTTAGTACAGGTATTCTTGAAGAGAGGATGGCCTGTGGTGTTCCCCTTAATTTAGCTTTTATGTTAAAAAAATTTTTACTTTTGATTTAAAAATT * 13520 * 13540 * 13560 * 13580 * 13600 * 13620 * 13640 * | 13660 * 13680 Pohi-Kino- : TTATGTGGCTTTTTTCTCGGTAGTTTTGTGGGACAAATTTTGGTTTTTTGTGGAACTGGAATTTGATTTACTAGTATGTATATCATACAGTGAGTTATGATGTGGATTGTTTGTCGGTTAGGTGTAGAAGGGTTGCACGTTAATTTTTCGTGTTAGAGGGAGTTTAAAAAATTATCCGAT : 13680 * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 * 13820 * 13840 * 13860 Pohi-Kino- : GTTCGTTGTTAAATAATTCGTAAGGGGGGGGTATTGTGAAAAAAAAATTTTTTGTAAATTTGTAGCTTAGTACAGGTATTCTTGAAGAGAGGATGGCCTGTGGTGTTCCCCTTAATTTAGCTTTTATGTTAAAAAAATTTTTACTTTTGATTTAAAAATTTTATGTGGCTTTTTTCTCGG : 13860 SRU1(117) * 13880 * 13900 * 13920 * 13940 * 13960 * 13980 * 14000 * 14020 * 14040 Pohi-Kino- : TAGTTTTGTGGGACAAATTTTGGTTTTTTGTGGAACTGGAATTTGATTTACTAGTATGTATATCATACAGTGAGTTATGATGTGCTTTCCTTCCAATCTGAGGTTGTTGAGTAATAATATTAGAAGGGGATAAAAGGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAG : 14040 SRU2(117) SRU3(117) * 14060 * 14080 * 14100 * 14120 * 14140 * 14160 * 14180 * 14200 * 14220 Pohi-Kino- : CCGAGCCCGGTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAGGGGATAAAAGGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAGCCGAGCCCGGTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAG : 14220 SRU4(117) * 14240 * 14260 * 14280 * 14300 * 14320 * 14340 * 14360 * 14380 * 14400 Pohi-Kino- : GGGATAAAAGGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAGCCGAGCCCGGTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAGGGGATAAAAGGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAGCCGAGCCCG : 14400 SRU5(117) SRU6(117) * 14420 * 14440 * 14460 * 14480 * 14500 * 14520 * 14540 * 14560 * 14580 Pohi-Kino- : GTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAGGGGATAAAAGGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAGCCGAGCCCGGTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAGGGGATAAAA : 14580 * 14600 * 14620 * 14640 * 14660 * 14680 * 14700 * 14720 * 14740 * 14760 Pohi-Kino- : GGTAGATGTAAAGTAGATTGGTTGTTAGGATATGAAATCCTCTAGCCGAGCCCGGTGTTGTTTGTTGGGCGAGGGGAGAGAGAGGTTGTTGAGTAATAATATTAGAAGGGGATAATCATTTGGGGGGACTATAGGGGGGCAGACCTATCCATATCTTATCTTCTAAAATATTTTGGGGAT : 14760 * 14780 * 14800 * Pohi-Kino- : TTATCTCGGCATTATTTATTGTGAGCGTTTTGTGAGCGTTTTTTTTGTGGGCTGAGAA : 14818 bp (2 LRU + SRU) ======================================= Hình PL3.3 Diễn giải hệ gen ty thể sán phổi Paragonimus heterotremus, chủng GX, Trung Quốc (Phet-GX-CN) / GenBank: MH059809 (13.927 bp) cox3(645) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Phet-GX-C : ATGAGATGATTGCCTTTATATAACTCTTGATTATTTTTTGTAGCTCTTTTGAGGTTATTTCTTTGGAAGCTTATTGGGGTCTTCTCTCTTCTGGGGTTGGTGACTCTTTCTATTTTTTTTTTGATTCGTGAGGTTATGCCTTATAAGGTTCATTACGAATTTGGCTTTTGGCTTTTTATT : 180 * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Phet-GX-C : CTTAGAGAGGTTGCTATTTTTGGTACTTTGTTTGTGATGTGTCTTTGGAGTATGGATGGTGCTACGGAGCCTATTTCTAGGATGATGGAGCTTCCTCTTCTGGGCTCTTTTTTATTAATTGGTTCTTCCATAACCGCTACTACTTATCATCATTGCCGGGGTTTGGAGTTTAGCTGGATT : 360 * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Phet-GX-C : TTCCTAGTTGTTACTATTGTTCTTGGCCTTAGGTTTATCCTGTTGCAGATTTTTGAGTTTTATGACTGTGAGTGTGATGTTTTAAGGAATGTGTACTATGCGGGGGCCTTTTCTACGGTGGGTTTGCATTTTAGTCATGTTTTTGTGGGAGTCTCTGGTCTGATTGTTTTAATGATGTTG : 540 trnH(His)67 cob(1119) * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Phet-GX-C : GGCGCTAATGTGGTTAAGCAGTGTTATGTTAAAGTTGCTGTGTGGTATTGGCATTTTGTGGACTATGTGTGGTTGTTTGTTTTTCTCTTGCTTTATTTTTTGTAGGTTTCTTCTCTAGGTTAATTTCGTAAACTACTGGTTTGTGGGGCCGGAGATCTTGGTTTTAGGAGGGGAACGTAT * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Phet-GX-C : GCTGTCTTTGTTGCGTCATAAAGTTGTGGATTTGCCTACGAATCTGTCGTTGAGGTATTTTTGATGTGGAGGTTTTATGATTAGGAGGTTCTTGGCATTGCAATTGGGCTCGGGGGTTGTGCTGTCTTTGCTTTATGTAGCTGATTCGGATATGAGGTTTGGTTGTGTTTTGGATTTTAC * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Phet-GX-C : GGGAGAGAGCTTATTTGTATGGCTTATTCGCTATCTTCATATCTGAGGTGTTACTTTTATCTTTGTTTTGTTTTTCATTCATATGGGTCGGGCTTTGTACTATTCTAGGTATAGGAAGGTGGCTGTTTGAAAAGTTGGTTTTATTCTGTATCTTTTGATGATGGTTGAGGCTTTTTTGGG * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Phet-GX-C : GTATATTTTGCCTTGGCATCAGATGTCTTATTGGGCTGCTACGGTTTTAACCTCTATTCTTAACAGGGTTCCAGTGGTTGGGGGCAGGGTTTATACTTTTATAGTTGGTGGCTTTGGTGTGACAAATGCTACGTTGGTTCGGGTGTTTTCTGCGCATGTTTGTTTAGCTTTTGTTATCGT * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Phet-GX-C : TGGTTTTAGGGTTATTCACTTGTTTTATCTTCATAAGGCTGGTTCTAATAATCCATTATTTGTGAGGGGTGGTTATAGGGATGTCGTGTTGTTTCATAGGTTTTTTACTAATAAGGATGGTTTGGTGTTAATGGCTCTTTTGATGCTGCTTAGGGGTTTTATGTGGGTTTGTCCAGATTT * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Phet-GX-C : GGTGTTAGATGTTGAGAGGTATTTGCAGGCGGATCCTTTGGTGACGCCTGTTTCTATAAAGCCTGAGTGGTATTTCTTGGCCTTTTATGCTATGCTTCGTTCGATAGAGTCTAAGATTGGTGGACTAGTTTTGGTGTTAGTTTTTCTTTTTATTCTTTGATTGCCTAGGTTTAAAGGCTC * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Phet-GX-C : CTGTTCCTACTTTTTGGGTCGTCAGTATATTTTCTGGGGCGTTTCTTCAATGTTCTTCCTTCTTAGGTATTTGGGGGCCTGTCATCCAGAGGATCCTTATGTTTTAGTTAGAAAGCTTTGTAGTCTTTTTGTGGTGGTTTTTCTGTTTTTGTTTAAGGGGTTGTGGGTGGTTCCTTATTC : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 nad4L(264) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Phet-GX-C : TTTTGGTGTCCCTTCCGGTGGAAAGGGTGCTTTGTAGAGTTTTGTGCGGGATGAGGAGCAATGTTTTGTTTTTGGGGAGCCTGGTTGTGCTATGCGGCCTTGTTTTATCTTTAGCTCGTTTTTTGAATTGCTTGATTATTGTTGAGAATTTTAAAGTTTTATTGCTCTTTTCGGCGATCT : 1980 nad4(1260) * 2000 * 2020 * 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Phet-GX-C : CTCAGCAGTGGCAGGAAAGCCACATTCTGTTTATTGCCTTAATGGTGATTTTTACCGTTGAGGTTGTCTTGGGTTTGGTTGTGCTTACTCGATTGTGAGATTTGGGAAGTTTGATTGGGTTAGTTGGTTGATAGGTGTAGGGATTGTTGGGGGCTTGTTCTTTGTTGGTGGCGGTTTGTG * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 * 2320 * 2340 Phet-GX-C : CGTTCCTTTAGTGGGAGGTCTAGAGGGTTTGGTTTTTTGTTTTGATGTCTTGAGGTTTTATCTCGGAGGGTTGTCGTTAATTTTGGGGTTGTCTCTGATTTTTTGGCAAAGATGTTTTAGGGGGGCATCCCGTTTTATGATTTCTTTGAGATTGTGTTGTTCTCTGCTCAGGTATTGTTG * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Phet-GX-C : TGTCCACTCTCTGGGGTTTTGAGTTTTTTATGAGGGTGCAATATTGCCGCTCCTATTTTTATTGATTCGGGAGTCACCATACTCGGAGCGGTATGTGGCGGCTTGATACCTGTTGGGATACGTTGTTTTAACTAGACTCCCAATGCTCCTTTGTTTGTTCTATCTTTCTGTGGAAGTTGG * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 Phet-GX-C : AAGGTTTAGGCTGTGTTATTGAGGGGATGTTTCCTTAGGGGGGTTTAGGGTCATCTTGTTGTTATCGGTGCTCTTTATAACTAAGATTCCGTTACCCCCTTTTCACGTTTGGTTGCCAATTGTTCATGCGGAGGCGAGGAGTCCTGTGTCGGTTTGTTTGAGGGGTTACATAATGAAGTT * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Phet-GX-C : GGGGCTGTTGGGGGTGTATCGTTTTTGTTCGTGATTGTTACCTAGACATGTTTTTTCTGTTTTTTACGTGGGGGTTTGCGTGGCGTTGACTCTTCTGTTTTTTTTTAGGGCTTCTTTAGAGTTGGATGGGAAGCGTTGATTGGCATTTATGAGGTTGGCTCATATCTTGGTTCCTGCGGT * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Phet-GX-C : GTGTCTTTGTGTTAGGGGGTTCGACAATTTAGGGGTTTCCTTTCTTTATTGTTTGGGTCATGGGGTTTCTGCGGCCGCAACATTTCTCTTTTTGTGGTTGGCTTATGAGGTATCGGGTAGCCGTAATTGGGCTGTGTTGAAGTTTAGGTTTTCTAGGAGTTTGTTGATGCGGTTTTTGGT * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Phet-GX-C : TGGAGCATGTCTTTGTACGGTGGCTTCTCTTCCACCCACAGTGCAGTTTTTCTGTGAGGTTCATACTTTGTGAGAAGCTGGGCCGCTTGCGGCTTGTTTTGTTCTTGGTCTCTTCTGTTATTTATTTTGTGGGGGGTTGGTGCCTATTTTTACGCTGGGGGGGGTGTTGTCTCGGCATTA : 2160 : 2340 : 2520 : 2700 : 2880 : 3060 : 3240 trnQ(Gln)65 * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 Phet-GX-C : CAGTATTGGGTTTGGTCCCGGGTCTATTTTTATGGGGTTACTTTCGGTGTTACTTCTTTTGATGTGGAGGTTTGTCTTGTTTTTGATTGGGTAGTAGGGTGATGCGGATACAGCTTGGTGTTTGTGTTGCATCTTATGTTTTGGTCATAGGGGTAGTTTGTTGCTAGCTGTAGTGTTTGG : 3420 trnF(Phe)66 trnM(Met)64 atp6(516) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Phet-GX-C : TCTAGGCTCTCGTCTAGCTTATTTTAAAGCACTAGTTTGAAGAGCTGGGGAAATAATTGTTTATGGGGGAGAGGCTGGGGAGGTAAGTTAAGTTATAAACTGTATGGTTCATGCCCATATAATACATTTTGTCCTCCCCTATGAATATGACTCGCTTAAAAGGTGTTGGAAGGTTTTTGT : 3600 * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Phet-GX-C : GAGGTCTGATAAGAGGCGGGGGTGGTGATTTTGTTTATCGTATCGTGTTATGTGGTCTGCTTTTTTGTTTTTTGAGCCTCCGTTTTCCTTATCTTTTTGGCTTGAGAGGATTTGGCTTGTTTCTTTTCTTCGTAATACTGCCCTTGTTTTTGTCTCTCTTTCTTTCTCGTCTCTTGGATG : 3780 * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Phet-GX-C : GTGGGGTTGTTTCGTTTTTCTCCTCGTTTGTGCCGGAGGGAACGCCTCTTTGAATTGCTCCATTCGTTTGTATGGCGGAAACTCTAAGTTATCTGGTCCGTCCGGTGGTTTTAATGATTCGTCCATTTGTTAATCTTTCCATGGGTGCGATGGGAGGCTATGTTTTGGGTTTGCTCAGGT : 3960 nad2(867) * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Phet-GX-C : TTCGCTTGGAGTGGGTGGCTTTTTTTTTGTTTCTCCTTTTTTTTTATGAGGTCTTTGTTGCGATTGTGCATTGATTTATAGTTTGTAACATTTTGTCTTTCTCCGAGGATCATTAGATGTGGGGGTATTTTTTGAGGTTGGTTAGCATAATGGGGGTGGTTTTTTTTTCTGTTTGTTTGT * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Phet-GX-C : TTTTAAGGGATAGCTTGGGATTGTTCTGGTTGTTTCTAGAATTGAGGACTTTGTCTCTGGTCCCGTCCTTTTTTTTACGTTTGGGGGGTGGTGTGTTGGAGGCTTTGTTTAATTATTTGGTTGTCTCGAGGATTTCTTCTTCTTTGATGATATGTGGGTTCTTGTATGACAGTCTGCTTT * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Phet-GX-C : TTCTTTGTTTTTTGGGTTTGTTGGTAAAGTTTGGTGTTTTTCCGTTTCAGGGTTGAGTTTATAAGGTTTTGTTAAGGTCCGGTTGAGTCGTTGTGTGAGGTTTTTCTGTTTTTTTGAAGGTTCCTTTTTTGTTTATGTGCTTTTTTTTGGGCAGTGGTGGAAGACTTCTTTTGAAAGTGG * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Phet-GX-C : CCTGTGTCTTGTCTTTTTTTTTGTTGGGAGGGTTATTTTGGTGTTATAGGTTTAGTTGGTGTCATTGCTGATGTCATATGATGCTCTCGTCTAGTGCTGCGTTGATGGCTATGTCGGTTAGCGGTTCAGCGGACGTCTTATTCTATCTCTTTGTTGTTTATGGTTTTTGGAGTAGGGCTG * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Phet-GX-C : TTGTGTTATTTCTTAGCCATCTCGAGGATATGGGTTTGACGGGAGTGGGGGCCTATTTTCTGTTTTTGATGTTGTTGGTTTCTTTGCCTATTTCTGTTTCTGTTTTTTACAAGGTGTGGATGGCGGTTGGAATCTATTTCTGCTATTTTCCGGTTTTTGTTGCGTGGTGTGTTTATAGTG trnV(Val)64 : 4140 : 4320 : 4500 : 4680 : 4860 trnA(Ala)68 * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Phet-GX-C : TGTCGGAACAGTTGTTTTTGTTTAGGTGGCTTATTAAGGATAGTGTGGCGTGTGAAACTTATAGGGGCGTTTTGTTGGGGTAGTTGGCGAGATAGTTTAATTAAAAACGTCTATCTTACACGTAGGAGTTGATTTGGTGTCTATCGCCAGAATATGGGAATGGCATAGTTTAATCTTAGA : 5040 trnD(Asp)66 nad1(906) * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Phet-GX-C : GATGGCTGCTCTGCAAGTAGCCGGTGAGGCTGGGCCTCTGCCGTTGGAAGGAGGGGAACGGTCTTAGTTTAGTCGTAGAATAGTAGCTTGTCGTGCTATAGGTGGGTTTAGTCTCAGGCTGTGATGGTTTCTTTGTTGGTGAGGAGGTATCTGTTTTTAAGGACGTTTCTTTCTTTTGGT * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Phet-GX-C : TTGATTATGTTGTTTGTGGCCTTCTTTATTTTAGGGGAGCGTAAGGTGTTGGGATATATCCAGTTTCGGAAGGGGCCTAATAAGGTTGGTGTGAGGGGGTTGTTGCAGAGTTTTGCAGATCTTTTAAAGTTAGTGATAAAGTTGAAGGTTTCTTCTTTTCAGGGTCGTAGTTGACTTTCT * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Phet-GX-C : TGATGGGGGGTGTATGCTTTGATATTTTTGGGAAGGCTTTACTGCGTTTTTTTTTCTCTTAGTCATTCTGGTTTAAGAAGAAGTAACAGTGCGCTTTGGTTGTTGGTGATAACTAGCATTACTGGTTATAGCTTATTGAGGGTTGGTTGAGGATCCTATAAAAAGTATGCTCTGCTAAGG * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Phet-GX-C : TGCTTGCGTTCTGCATTTGGTTCTATTACTTTTGAGGCGTGTTTCATGTGTGTTGTTCTTTTATTGGCTATTATTAGTGGCGTCTACTCGTTGGGCCCTTACTTGGAGCGCTCTTTTCTTGTTTTTTTAGTCTTGCCGTCTTGTTATGCTCTTTGGCTGGTTGGGATTTTGTGTGAGTGT * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Phet-GX-C : AATCGTACACCTTTGGACTATGCGGAGGCTGAGAGAGAGCTTGTCAGTGGTTTGAATACGGAGTATTGCAATGTGCCGTTTACGTGTCTTTTCGCTTGTGAATACCTGATAATGTTTATTTTCTCTTGGTTAGGGGGTGTGATTTTTTGAGGAGGTGCTGGTGTGTTGATGTTGTCGGTT : 5220 : 5400 : 5580 : 5760 : 5940 trnN(Asn)71 * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Phet-GX-C : CTTCATGTTGTTTTTTTTATTTGGTCTCGTGCTACGTTGCCGCGGATCCGTTATGATTATTTTGTTCGGTTTATGTGGAAGTGGGCTGTGTTGGTGCTTGTTTTTTCTTTTTTTTTGGTTTTGGTGTAGCGGGGTTGTGTAGATTATTTCTTTAAATCCTAAGGCTGTTAACCTTAAGAA : 6120 trnP(Pro)66 trnI(Ile)62 trnK(Lys)65 * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Phet-GX-C : GAATTTTGTTGATTCTGCGGCCGCAACAGAGTAGTCTAGTTTTGAGGATTCGAGTTTTGGGGACTTGGGGCCCCGTAAGGGCTTGTTGACATATTGCCGATTGGGCTGCGCTAGCAGGCTGCTGTGATATAGCGGTTGTAGGATTTTTCTTCGTCGGTAAATTTCTGAAAGTAGCTTATT : 6300 nad3(357) * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Phet-GX-C : TTTAAAGTTTGGGATTCTTACTCCTAGGATGTTGTTTTAACCTTTTGGGGTAATGGGGGTTTTGCTTTGTGGCGTGGGTTTAGGGGGTTTGCTTTTTTTTCTGGTTTGTGTGTTTCATGGCTTTTTTTGGAATGTTGGTGAGGGTTCTAGGTCTGGTCTTCGTTCTTGGGTAAGTTCTTT : 6480 * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Phet-GX-C : TGAGTGTGGTTTTGTATCTCAGCGGGGGGTGGAGGATTATTTCAGACATACTTATTTTGTCTTGTTAGTTTTTTTTGTTATCTTCGATTTGGAGGTCTCTTTATTGTTAAAAATGCCTTTGCAGGGTGTTTTGTACAAGAATTTGGGTTTTTATGTCCTTTTTTTGCTTTTGTTAGCGCT : 6660 trnS1-AGN(Ser)60 trnW(Trp)68 * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Phet-GX-C : TGGTTTTGGTGTAGAGATTTATAAGGGTTATGCTGAGTGGGAGTATTAGGGTTTTGAGAATTGGTTAGTTGTCGCTGCTAACGATGATTAGGGCATTTAATTTGTCCGGTTCTCTGTGAGAATGGTCTAGGTTATTTTTTTGACTGTCTGTTTTCAAAACAGAAGGGGGCTTTGTAAGCC : 6840 cox1(1536) * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Phet-GX-C : GTCCGTTGGTTATGTTTACTTGGTTGTTTACTTTAGATCATAAGCGTATAGGTGTGATTTACATGGTCTTGGGTGTGTGGGGTGGATTTTTGGGGCTCTCTTTAAGGATGTTGATTCGCTTGAACTACTTAGATCCTTACTACAATATTGTTTCGTCGGAGGTTTACAATTACGTGATAA * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Phet-GX-C : CGAATCATGGTGTTGCTATGATTTTTTTCTTTTTGATGCCTGTCTTGATAGGTGGTTTTGGTAATTATCTTCTTCCGTTGTTGTTAGGTATTCCTGACCTGAAACTTCCTCGTCTGAAAGCTCTTAGGGCTTGATTAATGCTTCCTTCGGCGGTTTGCCTTAGTGTGAGAATGTTAGGAG * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 Phet-GX-C : GTGCGGGAGTGGGGTGAACTTTTTATCCTCCTCTTTCGGGTGGTGATTATTCTGGTTGGGGTACGGATTTTTTGATGTTTTCTCTTCATTTGGCTGGTCTTTCTAGTCTTTTTGGTTCGTTGAAATTTATTTGTACTATCGTCAGGGCATTGTGTGAGCATACTGTGGGTCGGAGGTCTA * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 Phet-GX-C : TAATTGTTTGGGCCTATCTTTTTACGTCTATCTTGTTGCTTCTTTCTTTGCCAGTGCTTGCTGCTGCGATTACCATGCTTTTGTTTGATCGAAAATTTAGTTCTGCTTTTTTTGACCCCCTTGGCGGCGGGGATCCTGTTTTATTTCAACACCTTTTTTGGTTTTTTGGCCATCCGGAGG * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * 7740 Phet-GX-C : TGTATGTTTTAATTTTGCCTGGATTTGGTGTTGTGAGACATATCTGCATGACTTTGACTAATAAAGATTCTTTGTTTGGTTATTATGGCTTGGTTTTTGCCATGGGGGCTATTGTGTGTTTGGGGAGGGTTGTTTGAGCGCACCATATGTTTATGGTTGGTTTAGATGTTAAGACTGCTG * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * 7920 Phet-GX-C : TTTTTTTTAGTTCTGTTACTGGGGTGATTGGGATTCCCACAGGGATTAAGGTTTTTTCTTGGTTGTTTATGTTGGGGGGCACTCGTTTACGGTTTTGAGATCCGGTGGTTTGGTGAATTTTAGGCTTTATTTTTCTTTTTACTATTGGTGGTGTAACTGGGATTATTTTGTCTTCTTCTA * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * 8100 Phet-GX-C : TTTTGGATAGTCTGTTACATGATACCTGGTTTGTTGTTGCCCACTTTCACTATGTTCTATCTCTGGGATCTTATAGAACTGTGGTGATATCGCTCTTGTGATGATGGCCGGTTGTCACCGGGTTTAGCTTGAATAAGTATTTGTTGCAGGGTCACTGGGTGGCATCGATGGTGGGGTTTA * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * 8280 Phet-GX-C : ATCTGTGTTTTTTCCCTATGCATTATCTTGGGGCTTATGGTCTTCCTCGGCGGGTGTGTAACTATGAGCCTGCTTTTCAGTGGCTAAACAATGTTTCTTCTGTTGGTGCCTTGGTTTCTGTGGTTAGGGCCTTTTTTTTGGTTTTTATTCTATGGGAGTCTTTGGTTGTGGGTAATCGTG : 7020 : 7200 : 7380 : 7560 : 7740 : 7920 : 8100 : 8280 trnT(Thr)64 Phet-GX-C : Phet-GX-C : Phet-GX-C : Phet-GX-C : Phet-GX-C : Phet-GX-C : trnC(Cys)65 Phet-GX-C : Phet-GX-C : Phet-GX-C : Phet-GX-C : rrnL(974) * 8300 * 8320 * 8340 * 8360 * 8380 * 8400 * 8420 * 8440 * 8460 TTGTTGGTGTTTGGGGTACTAAAGCCCTGGTTATGAATGCTTTAGTTACTCCAGTCCCCCATCATAGTGTTTATATCAGTGGGCCGTCTCGTTGGCTTTCTTTTTAGGAAGTGTAGTTTATCTCAGAATGCTGTTTTTGTAAAGCAGTGGTGTGATCTTGTCGACTTCTTTGGTTTTGAG * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 * 8640 GCCGTAGTCTAGTTCTTGTGTTTAGTAGGTGTCGTACCTTTTGCATCATGATTCTTTGAGGGGGTTTAATTATCTTATTATTCCGAAAGGTAGCGATTTAAATCTGTCTTGTTTTAGGGCTTAGGGGTTAGCGGGTAGCTAATCTTTAGAGATGGGTTTGGAGGCGATAAGTAATGCGTG * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 * 8820 CTACTTTATAGCTGATTGTTGAGTGTTTTTTGTTTTAACTTCGTTGCTTGGATGTGGCGAGGTGTTTAAGAGAGTTTATGGGGGTAGACAGAGGCGGGGTTAAAAGTACCCTGTTTTAGTTTCGTGTTATAACGATCGTCTTCTCTAGGTCCTCTTTAGTTTAGCGCTTGACTTATTTTA * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 * 9000 ACAGTGTTTTGAAGTATGGGGGGATAAGTAGTTTAAATGGTTTCTTTCTTTCTCGAAGCTCTCCGGTCTGTTTCTTAAAAACATTTCCATCATCTTGTGAAGTTTTGATGGTAGTGCCTGCTCGATGTTTTAATAAATGGCCGCAGTATTTTGACTGTGCTAAGGTAGCATAATTAGTTG * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 * 9180 CCTTATAATTGAAGGATTGTTTGAATGGTTTGACTAGGGGGGTGTTTTAGATGGGGGCTATTGTTGAAATTAGGTGGCCGGTGCAGATCCCGGTGTTTTTTAATTAGACGGAAAGACCCCGAGAGCTTTAACATTTTTGTTTTACTTGGGGCAAGGGCATATGCTTCATATGCTTTTGGA * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 * 9360 TCCTTTGGGAAAGAGGTTTAGTTACCTCGGGGATAACTAGGTAAGAGACGAGGAGAGGTCTTATCGATTTGTCTGTTTGCTATCTCGATGTTGACTTAGGGTTAGCATGGGGGTGTAGGAGCTCTTATGTTAGGGTCTGTTCGACCGTAGTTCCCTTCATGAGTTGAGTTAAGACCGGCG : 8460 : 8640 : 8820 : 9000 : 9180 : 9360 rrnS(737) * 9380 * 9400 * 9420 * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 TGAGCCAGGTCGGTTCTTATCTTTTATTGTTGCGGTTTCGTACGAAAGGAGCATCCGTAGTTTAGGTTGGGTGGGATATGTGTCTTGCATAGTTTACTCTGCAAAGGTGAATTGGGCCTTTTGCCCCCACCCTGGTTCAATTTAATTCTGGTTGTGG bAAGAGATGTTAATCAGTGCTACA * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 TAGGTTCTGTTTTGAGGTCAAAACCAATAGATTTTTGGTAGTTGAGCCTAGTGGTTGGGTCTTGGTTTTTGAGGTAACTCAGATTGGGCTGTTTTAATTGGGGTGGATAGTTCGCAGTGCCAGCATCCGCGGTTATTCTGTTAATCCCCTCTTCTGTTTGGTTTAAATGTTTTGTAGGGT : * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 ATTCTGACTTTTGGGTAGAATTCTTGTCTTGTTGAGATATGCCTACATTTCTTGGAGTGTTTTTTTAGGAAAAGGATTAGAGACCCTTGTACTAGGTGGAGTATTTTGTGTCCATGGTGTAAACTAAAAGGATTTGGCAGCCGACGAGGTTCTTCCGGGGGAGTGTGTGGTTTAAGAGAT : * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 AGTCCGCTTAGGATCTTGCCGCCGCTATTAGTTAGTGTATATCCGGTTTAAGATTCGTGATTTGTGTCATTGAGTGTCATTTTATTTTAATTGAATCCAGGTCAATGTGCTGCTTATGCGGTGGTGCTTATGCACTACTTTTAAAAGGTTCCTTGGTGTAATCTGGGGAGGTTTAGGACT : : 9540 9720 9900 10080 cox2(600) Phet-GX-C : Phet-GX-C : Phet-GX-C : Phet-GX-C : * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 * 10240 * 10260 CGGAAGTAGGTGGATTGAGGTTTGTTCTATCGAATGTTGGATTTAGTTGGGGTACATACCGCCCGTCACCCAGGGTGTTAACTGTGGTAAGTCGTAACATGGTAATGTTGGGGGAACCGGACATTAGTTGGTATGGCTCGTTTTAGGGCGTGCCTATGTACGTGTATACGCCTCTGTATT * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 CTGAGATGGTGGGTTATATTTCTTTCTTGTCCGCTTTTATTCCTATCTGGGTTTTTCTGGTTTTGGGTTGGCAGCTGTGTAGTAGGTATGGGACCGCGGCGCTTCGTAATGAGAGGGATTTGTTAGAATTTTTTTGGACGGTTGTTCCGAGGGTTTGCGTAGGGTTTCTCTGTTTTTTGA * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 ATTTGGGTTGTTTGGGTAATGATTTAATCTTAAAGACGCGTGGTCTGGTTAAGGTGATTGGTCGTCAGTGGTATTGGAGTTATGACCTAAAAGATGGTCAGGGGTTATACGATTCTGTGATGAGAGATTTTATCGATGGGGTAGATAAGCCTTTGCGTGTTTATGCGGATACCCCTTATA * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 ATCTTTCGATAACGTCTAGAGATGTCATCCATTCTTTTGCCCTTCCTGTTGTTAACTTGAAGGTTGATGCAATACCGGGACGCTTGAATAAAACTTTTTTCTGTATGAACCATGTTGGTATTTTTGTTGGTTACTGTAGAGAGTTGTGTGGTGCAGGGCATGCATACATGCCTATAGTGG nad6(453) : 10260 : 10440 : 10620 : 10800 * 10820 * 10840 * 10860 * 10880 * 10900 * 10920 * 10940 * 10960 * 10980 Phet-GX-C : TGGAGGCTGTTGAAAAGGCCTTTATTAAGGATTAGGTGAAGAGTTTTCTTTTAAGTCTCTATTTTTCGAGATTGGTTGGGTTTTCTTTCGTCGGCCATCCGGTGGTTTATTGTATTCTCTTGTTGGCGAGTGCTTTAAGAATTAGTGGGTATTTATACCTTGTGTTAGGTATGTCGTGGT : 10980 * 11000 * 11020 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 Phet-GX-C : ATTTAGCATTGTTTTGTTTGGTCTATGTTGGTGGTATTTATGTCCTATTCATTTTCGTCTCCGTGCATATCTCTAATCCTATGCCCGCAAGGGGTAGGAGGGGGTTTGTGTTTTTGGCAACGCTGGGATTATTTTTGCTATTTTTGGGGAGAGGTCTTGATCGCAAAGTTATGGGATTTT : 11160 trnY(Tyr)63 * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 * 11300 * 11320 * 11340 Phet-GX-C : GTGAGGAAAGTCATTACCTGTGTTCCTATTATGAAGGATTTTCCTATTGTTTATTTTGCTTGGTCCTGATGATCGGTTTTGTTGGTGTCAGCATCGTTTCGGGTGAGAAGGATTCCTTTTTTCGTTAAGCTCGCCGGCTTAGTATATTTTTGAGTGCGTGAGATTGTAAATCTTGAGGGG : 11340 * trnL1-CUN-66 11360 * 11380 * 11400 * 11420 trnS2-UCN-63 * 11440 * 11460 * 11480 * 11500 trnL2-TAA-65 * 11520 Phet-GX-C : GTTTTCCAGTCGGAAGTTGGGACATTAAGAGTGCCAGATAAAATGGGTTTGATTTAGGATTAAATGATGGCTGTTTTTTGCCCTCTTGCGGGCAAGGGGTTCCAGGAGGAATTTGAAATTCTTTCTTTCGTTTAGTTAACGATGCCCGCTTGTATGTTTGTTTAAAAGGGCACACGGGTG : 11520 trnR(Arg)67 nad5(1578) * 11540 * 11560 * 11580 * 11600 * 11620 * 11640 * 11660 * 11680 * 11700 Phet-GX-C : CCAGATAAAATGGGTTGGTTTTAAGCATCAAATATGAGGATTTTCTTCCCCGTGTGGTCTGATATCCTGAACACGGGATTACGTTTCGGCCGTAATTGTGAGCAATGTTGTTGTTCTATCAGAGGTGTTGGGGTTGAGGATGATGTTGGTTTTAGGGATGCTGGTTTTCTGGGGGAGTAA * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 * 11840 * 11860 * 11880 Phet-GX-C : ATGAAGGGGCCTGGAAGCCTCTGTAAAACTTTGGAGATATTGTTGGAAGGAGGGTAGAGAGTTATTGGTGTTTGATGAGGTTAGTTTGATTTGTTTTTTCATGCTTTTTTGTTGTGGCAGACTTGCCCTTTTATACTGCTACCACTACTTAGGGGTTTCTTTGGAGGGAGAGCGTCTATT * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 * 12020 * 12040 * 12060 Phet-GX-C : CCCACTGATGGTTTGGTTTCTGGGCGTGATGGGTATTTTAATTTTTAGCGGTAGTCTCGTTTTTTCGTTAGTTTTATGAGAATACCTGGGATTGGTAAGATTTCTGTTGATTTTGTTTTATTCCAACAGGAGTAGCGCCCGGGCTGCCCTTGTAACTTTGTTTGCATCTCGATTCGGCGA * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 * 12200 * 12220 * 12240 Phet-GX-C : TGTTTCGCTGTTTATGCTAATTCTTTGATGTGGTGTGTGGTGAGGGAATAGGGGTTTCCTTTTTTTCTTTTTTTTTCTCCTCGTTGTTTTGACTAAGAGTGCGGCGTATCCTTTTATTTCTTGACTTTTGGAGGCCATGCGTGCTCCGACCCCGGTGAGCTCTCTTGTGCATTCTTCTAC * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 * 12380 * 12400 * 12420 Phet-GX-C : TCTTGTTGCTGCCGGGGTTTGGTTTCTTTTGCGTTATCAGGGGTCTGTTGATTCTGTAGTGAGTGGGGTGTTGTGTTTGGTTTGTATCTTGACGATTGTTGTGAGTGGCGTTTGTGCGTCTCTTTTTAAAGATTTAAAGAAGGTGGTTGCGCTGTCTACGTGTAATAACATTTCTTGGTG * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 * 12560 * 12580 * 12600 Phet-GX-C : TGTGATTTTTTTTGTTTGTGGGGATTTGGCTCTTTGTTTGTTGCAACTTTTAACTCATGGGGTTTGTAAGTGCTATCTTTTTATGTCTGTGGGGGATCTTATGTCTCAGTCTGGTGGGAGCCAGAGGTCTGTAGGTGTTTATGGCTCTCGTTATACGGGGGTGTTTGGTCCTTTTTTGCA * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 * 12740 * 12760 * 12780 Phet-GX-C : AAGTATTTTGATCTTTTCTTTGTCTGGTTTACCGTTTATGGGTGTCTTTTTTAGGAAGCATGGTCTTTTTTCGGTTGTTTCCTATTGTTATGGGGTTGGGTTTGTGCTGGTTTTTTGGTTGGCTTTTTTCCTGACCTATGTTTACTCTGTTCGTTTAAGGTTATTGCTGTTGAAGAGTTT * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 * 12920 * 12940 * 12960 Phet-GX-C : TAGTGGATTGTCCAGGGGTTATTCTAGAGCGTTTTTTTGCATAGGGGGTTTATGTCTGTTAGGGATTTTTTTGAATTGGATGGGTTTAGTTTTGTTTGATGAGAGGTTCGGGCTAAATGTCTGGTGGAGAATTATTATGCTTTTGGTTCAGGGTCTAGGTTGTTTGTTTGGTTGAATTCT * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 * 13100 * 13120 * 13140 Phet-GX-C : GTTTAGTGGTGTGGGGGGTTTTAGATGGGGGATTATTTGAAGTTCTTTATTGTGGGGTAGAGATTGTTTGGTAAGGGCGTTTTATGCTGTTTTTTTGGGTTTATCGGAGGTGGCGGTGCTTTCTTTTTATCGTTGGGAGCTTTATGGTTTGGGGCTTGTTCCGGCAGGAGGCGGTTTTTT trnG(Gly)68 : 11700 : 11880 : 12060 : 12240 : 12420 : 12600 : 12780 : 12960 : 13140 * 13160 * 13180 * 13200 * 13220 * 13240 * 13260 * 13280 * 13300 * 13320 Phet-GX-C : TAGAAAGGTTTTTACTTCTTTGAAATTACTGGTATTGGGGGTGGTTTTTGTTGTCTTCTTATTTTCCTTGTCTTTCTGTTAGTTAGGGTGCGTGTTAGTAGTATAAGGTTAGAGTATTCTGTCTTTCCAAGTCAGAGGTCCGTTTATTTGGTTAACATATGTTGGGTGGTTTGAAGCGAT : 13320 trnE(Glu)66 * 13340 * 13360 * 13380 * 13400 * 13420 * 13440 * 13460 * 13480 * 13500 Phet-GX-C : GGGGCCGTTTTAGCTTTGTTGTGTCGGTTAGGTGTAGGAGATGCATGTTAATTTTTCGTGTTAGAGGTAGTTTTAAAGTTATCCGACATCGGGTGTAAAATTTTTTGTAAGGGGGGGGTGTTGTGAAAAGTTTTTTTTTTGAGAATTTGCAGCTTTTTTCGGGTGTACTTGAGATGGCTT : 13500 * 13520 * 13540 * 13560 * 13580 * 13600 * 13620 * 13640 * 13660 * 13680 Phet-GX-C : TTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGGTGCATTCTTTAATCCTATGCCCGCAAGGGGTAGGAGCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGTGTTGGTTTATGCCA : 13680 * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 * 13820 * 13840 * 13860 Phet-GX-C : GGTCTGTCTCTTATACACATCTAGATGTGTATAATAGTCTTTCTTATCCCGAGCCTCTGTGTGGGGGGTGCGAGAAGGTATGTCTGGGGTTTATTACTAATATTGGGAGGACCTAGAGAAGAGAGATGGTTTGGGGGGACTATAGGGGGGCCGTAGGAGATATCTAACATATTTCCCCGC : 13860 * 13880 * 13900 * 13920 13927 bp ======================================= Phet-GX-C : CGTGCTGTGTTTGAGTCTTAGTGTGTTTGGTGGGCGAGTTGGGGCGTTCGAGTGATGTGACTAGGAG : ĐƠN VỊ MÃ HÓA RIBOSOME Hình PL3.4 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome loài Paragonimus heterotremus của Việt Nam (chủng Phet-LC-VN), thiếu IGS (7.661 bp; ITS1 chứa 7.7 TRU) 18S (1977) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 =PhD3-TUco : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACATACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 =PhD3-TUco : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGTGGCTTCGGCTGCTTCCGTTGTACTCTGTGATGACTCTGGATAACTTCACTGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTACGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 =PhD3-TUco : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 =PhD3-TUco : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTTTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 =PhD3-TUco : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGTTTTGCAGGTGCTGACTAGGCCTTCGGGTTTGTCGGCATGCTTCCAGATGCCTTTAAACGGGTGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 =PhD3-TUco : CATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTTCGGATCCGAAGTAATGGTTAAGAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 =PhD3-TUco : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGTGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 =PhD3-TUco : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAACGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 =PhD3-TUco : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCTCGTGCGGGTGGCGGTGCTCGCTGCcTCTTCGGGGGTGGTGGCGTTGTTCGCCGGCGGGTGCTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 =PhD3-TUco : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCCGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTTCAAGTCATAAGCTTGCGATGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (1371) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 =PhD3-TUco : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGTTTCGGCAGCTCGACCGGCGCTGAGAAGACGACCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACA : 1980 Int seq (75) * 2000 * 2020 * 2040 * TRU12060 * 2080 * 2100 * 2120 * 2140 * 2160 =PhD3-TUco : GAATTCCCAAAATTCAAAATGCCTCAATGAGCATATGGGCAAGTATTCTCCACGGGTAGTCATACTCGTTAAATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCACACTCGTGTGCCTACGTACAGTTAGATCTTTTCCGATGAGCCT * 2180TRU2 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300TRU3 * 2320 * 2340 =PhD3-TUco : TCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTAGATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTT * 2360 * 2380 * 2400 * 2420TRU4 * 2440 * 2460 * 2480 * 2500 * 2520 =PhD3-TUco : GATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTGGATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTAGATCTTTTCCGATGAG * 2540TRU5 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660TRU6 * 2680 * 2700 =PhD3-TUco : CCTTCGGGTTTGtTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTAGATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCA * 2720 * 2740 * 2760 * 2780TRU7 * 2800 * 2820 * 2840 * 2860 * 2880 =PhD3-TUco : TTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTGGATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTGGATCTTTTCCGAT : 2160 : 2340 : 2520 : 2700 : 2880 Int seq (349bp) * 2900TRU7.7 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 =PhD3-TUco : GAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCAGGTGGAGCGTCTCTCCTCTGCCATTTGATTCGACTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTATGCTTGCAGCAGGGTGCCTACCCGTCTGATGCTCTTTGATTTGCTTGCCATTTTCGGATGGTCAGTCCATCT : 3060 * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 =PhD3-TUco : CGGGAGTGACGGGTTGTGCTGTCCTCATAGACAGCGCTAGGCTTAATGAGTGGTAAGGCATATTGAGCTACGGCTCGACCACCGCCCTGTTTTGTTTCAATTTATGCCATTTTACACTGTTCAAGTGGTTCAGGTCGGCCTGTCTGACTTGGTCCATTGCCCGACATGCACCCGGTCCCG : 3240 5.8S (160) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 =PhD3-TUco : TACTGGACTGCATGTACAGTCGCCTGGCGGTGCCTTATCCCGGGCTTGACTGGTTAACCATACATCGTTCGTCTGGGTGACCGGATGTTCGGTGTGAGTACAACTCTGTGCGGTGGATCACTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGTGAACTGCATAC : 3420 ITS2 (283) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 =PhD3-TUco : TGCTTTGAACATCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTGCCAGCTGGCGTGATTTCCCCAACGTGGCCTTGTGTCTGTGGGGTGCCAGATCTGTGGCGT : 3600 * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 =PhD3-TUco : TTCCCTAACAAATCCGGGCGTATCCATGTTGTGGCTGAAAGCCTTGATGGGGATGTGGCAACGGAGTCGTGGCTCAGTGAATGATTTATGTGCACGTTCCGCTGTCCCGTCATCATCTATGGTTGAAGTTGCGCGTGGTGTGTCCGATGCTGACCTATATATGTGCCATGTGGCTCATTT : 3780 28S (3881) * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 =PhD3-TUco : TCCTGACCTCGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTACT * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 =PhD3-TUco : GCTCCACCCTAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAAAGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCGGGTTGTTTGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCAC * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 =PhD3-TUco : GAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGCAAGCTTTGAGGATTCAGCTGGTGAGTGTGGTTTGGGCTTGGTCAAGTCGGTTGGACATTGAGGTCTGTGTAGC * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 =PhD3-TUco : AGCAGGTCTCTGCCCTCGGGTAGGGATGCGCGATACACTTATCAAGTGTTGTGCGCCTCTTTGTTTATCGGGCCAATTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCACTGCTGTCTGACTACTGTGGTTAAACCGGACTTACAGAGCACTTGTGGCTTTGTATGGCCGGG * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 =PhD3-TUco : AAGGCAGGTAGCTCGTTGGCTAGCTTGTGGTTTACTGCAGGCGTTTGGTTTTCGAGTGTAATTAGCTGACCACGATTGTTCTGTGCAGTGTGTCGGAGACGGCGGCTTGTGGTACTTGCGTGCGTGCCGGTCCTGCTGGCGTGTTCGGGTTTGGTTGCCATGTTGCTTGTGAATGCAGGC * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 =PhD3-TUco : CTGGTGATAGCTCGGATTCGTTTGGTTGGCGGTTGCGTATGTGATACCGTTAAGGGCCAACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGG * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 =PhD3-TUco : CCTGGCTTGTCTAGGCTGAGGTGAGATCCTGTCGTTTTCACGCATGGTACCGCCAAGCAACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAGTCTTCGGACCAGTGCACCGTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCT * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 =PhD3-TUco : TGCGCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAATAAACAGTTTTATCCGGTAA * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 =PhD3-TUco : AGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAA * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 =PhD3-TUco : CGCGACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGAGTCGG * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 =PhD3-TUco : TAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAG * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 =PhD3-TUco : GGTTCCATGGGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTCGGTACAGAGACGGGGTGCCTGACATTGTCAGTATTGCTGCTCGTATTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTACAT * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 =PhD3-TUco : TCCGGTCTTGTACTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCACCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAAC * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 =PhD3-TUco : GCTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAA * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 =PhD3-TUco : ATGGGCTGGGATCAGATACGTGCCGGTCAAGTTGGAGGCTGGTTTTTCTGTTGCTCTGTTCGCTTCGGTGGATGGGGTTGATGGGGTTACTGGACTCTGACCAGGCCGGTCGTGGACGATCTCAGCTGTAGTGCTGCTTGGTTCGCATCGTGCGTGGGAAACCGCGTGCGTCTGTGGCCT * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 =PhD3-TUco : TGCTTCCTTCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGATCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGG * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 =PhD3-TUco : GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGA * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 =PhD3-TUco : CTTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGGTCTTTCGGGATTTGGCGTCAGTGAAATACCACCACCGTCATCGTTTCTTTGCTTATTCAGTAAAGCGGGGTGCCAATTTTGGTTCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTC * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 =PhD3-TUco : ATGTCAGATGAGCCGTGGTTTTCGGCTGCGGTGTTTGAGTTTGAATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTAAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAA * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 =PhD3-TUco : AAGCTTACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGATCCTTTTGATTTTGATATTCGGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 =PhD3-TUco : GCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGG * 7580 * 7600 * 7620 * 7640 * 7660 7661 bp ///-IGS-/// ======================================= =PhD3-TUco : AACCGCAGGTTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : : 3960 : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 : 7020 : 7200 : 7380 : 7560 Hình PL3.5 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome (rTU) loài Paragonimus ohirai của Nhật Bản (Pohi-Kino-JP), thiếu IGS (7.422 bp; ITS1 chứa 5,7 TRU) 18S (1977) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Pohi-Kino- : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACATACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Pohi-Kino- : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGCGGCCTCGGCTGCTTCCGTTGTACTCTGTGATGACTCTGGATAACTTCACTGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTACGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Pohi-Kino- : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Pohi-Kino- : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTTTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Pohi-Kino- : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGTTTTGCAGGTGCCGGCTAGGCCTTCGGGTTTGTCGGCATGCTTCCAGATGCCTTTAAACGGGTGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Pohi-Kino- : CATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTTCGGATCCGAAGTAATGGTTAAGAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Pohi-Kino- : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGTGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGgAAGTATGGTtGCAAAGCTGAAACTTAAAGGAATGGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Pohi-Kino- : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATGGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Pohi-Kino- : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCCCGTGCGGGTGGCGGTGCTCGCTGCCTCTTCGGGGGTGGTGGTGTCGTTCACCGGCGGGTGCTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Pohi-Kino- : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCCGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (1119) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Pohi-Kino- : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGTTTCGGCAGCTCGACCGGCGCTGAGAAGACGACCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACA : 1980 Int seq (77) * 2000 * 2020 * 2040 * 2060TRU1 * 2080 * 2100 * 2120 * 2140 * 2160 Pohi-Kino- : GAATTCCCTAAATTAAAAGTGCCTAGATGGGCAAATGGGCAAGTGTTTTCTAAGCGAGTAGTCATACTCGCTAAATGTAGCCTCGGCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGC * 2180TRU2 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300TRU3 * 2320 * 2340 Pohi-Kino- : CTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGTGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCAT * 2360 * 2380 * 2400 * 2420TRU4 * 2440 * 2460 * 2480 * 2500 * 2520 Pohi-Kino- : TCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATG * 2540TRU5 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660TRU5.7 * 2680 * 2700 Pohi-Kino- : AGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGC : 2160 : 2340 : 2520 : 2700 Int seq (349bp) * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Pohi-Kino- : CATTCGGTCCGGCTGTGCCTGGCGCATTCGTGCGCCTACGCACAGTCATGCTTTCAGCAGGGTGCCTATCCGTCTGATGCTCTTTGTTTTGCTTGCCATCTTCGGATGGTCAGTCCATCTCGGGAGTGACGGGTTGTACTGTCTTCATAGATAGTGCTAGGCTTAATGAGTGGTGAGGCA : 2880 * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Pohi-Kino- : TACTGAGCTACGGCTCGGCCACCGCCCTGTTTGTTTCAATTTATGCCGTTTCACACTGTTCAAGTGGTTCGGGTCATCCTGTTTGGCCTGTTCCATTGCCCGACATGCACCCGGTCCCGTACTGGACTGCATGTACAGTCGCCTGGCGGTGCCTTATCCCGGGCTCGACTGGTTAACCAT : 3060 5.8S (160) * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Pohi-Kino- : ACGTCGTTCGTCCGGGTGACCGAATGTTCGATGTGAGTACAACTCTGTGCGGTGGATCACTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGCGAACTGCATACTGCTTTGAGCATCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCC : 3240 ITS2 (285) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 Pohi-Kino- : TGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTGCCAGCTGGCGTGATTTCCCCAATCTGACCATGTGTTGGTGGGGTGCCAGATCTATGGCGTTTCCCTAACCTATCCGGGCGTACCCATGTTGTGGCTGAAGGCCTTGGTGGGGATGTGGCAA : 3420 28S (3881) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Pohi-Kino- : CGGAATCGTGGCTCAGTGGATTATTTATGTGCGCGTTCCGTTGTCCTATCATCATCTATGGTTGATGCTGCGCATGGTGTGCGTCCGACGCCAACCTACGTATGGGCCGTGTGGCTCATTCTCCTGACCTCGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGG * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Pohi-Kino- : AAAAGAAACTAACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTGCTGCTCCACCCTAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAA * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Pohi-Kino- : AGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCGGGTTGTTTGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGT * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Pohi-Kino- : AAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAATTGAACTGCAAGCTTTGAGGATTCAGCTGGTGAGTGTGGTTTGGGCTTGGTCAAGTCGGTTGGACATTGAGGTCTGTGTAGCAGCAGGTCTCTGCCCCCGGGCAGGGATGCGCGATACACTTATCAAGTGTTGTGCGCCTC * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Pohi-Kino- : TTTGTTTATCGGGCCAGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCACTGCTGTCTGACTGCTGTGGTTAAACCGGACTTACAGAGCACCTGTGGCTTTGTATGGCCGGGAAGGCAGGTAGTTCGTTGGCTAGCTTGTGGTTTACCATAGGCGTTTGGCTTCCGAGCGT * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Pohi-Kino- : AATTCGCTGACCACGATTGTTCTGTGCAGTGTGTCGGAGACGGCGGCTTGTGGTGCGTGCATGCGTGCCGGTTCTGCTGGCGTGTTCGGGTTTGGTTGCCATGTTGCTTGTGAATGCAAGCCTGGTGATGGCTCGGATTCGTTTAGTTGACGGTTGCGTGTGTGGTGCCGTTAAGGGCCA * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Pohi-Kino- : ACAGTCTGTGGTGTAGTGGTAGATTATCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGGCTTGGCTTGTCCAGGCTGAGGTGAGATCCCGTCGTTTTCATGCATGGTACCACCAAGCA * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Pohi-Kino- : ACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAGTCTTCGGACCAGTGCACCGTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAA * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Pohi-Kino- : ATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAGTAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAAT * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Pohi-Kino- : GGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAACGCGACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGC * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Pohi-Kino- : CATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGGGTCGGTAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAG * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Pohi-Kino- : GAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATGGGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTC * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Pohi-Kino- : GGTACAGAGACGGGGTGCCTGACACTGTCAGGATTGCTGCTCGTACTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGTATTCCGGTCATGTGCTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGG * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Pohi-Kino- : CAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCATCCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAACGCTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTA * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Pohi-Kino- : CCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGGATCAGATACGTGCCGGTCAAGTTGGGGGCTGGTTTTTCTGTTGCTCTG * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Pohi-Kino- : TTCGCTTCGGTGGATGGAGTTGATGGGATTACTGGACTCTGACCAGGCCGGTCGTGGACGATCTCAGCTGTAGTGCTGCTTGGTTCGCGTCGTGCGTGGGAAACCGTGTGCGTCTGCGGCCTTGCTTCCTTCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATC * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Pohi-Kino- : CGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGACCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGC * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Pohi-Kino- : ATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGATCTCTCGAGATTTG * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Pohi-Kino- : GCGTCAGTGAAATACCACCACCGTCATTGTTTCTTTGCTTATTCAGTAAAGCGGGGTACCAATTTTGGTCCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTCATGTCAGATGAGCCGTGGCTTCGGCTGCGACGTTTGAGTTTGAATGTGATGTGCGACC * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Pohi-Kino- : CGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTAAGCTCAGTCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGA * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Pohi-Kino- : TCCTTTTGATTTTGATATTCGGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATA * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 Pohi-Kino- : GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCT * 7400 * 7420 Pohi-Kino- : ACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : : 3600 : 3780 : 3960 : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 : 7020 : 7200 : 7380 7422 bp (5,7 ITS1-TRU) ///-IGS-/// ======================================= Hình PL3.6 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome loài Paragonimus iloktsuenensis của Nhật Bản (Pilo-Amami-JP), thiếu IGS (7.543 bp; ITS1 chứa 6,7 TRU) 18S (1977) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 PILO-JP-rT : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACATACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 PILO-JP-rT : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGCGGCCTCGGCTGCTTCCGTTGTACTCTGTGATGACTCTGGATAACTTCACTGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTACGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 PILO-JP-rT : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 PILO-JP-rT : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTTTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 PILO-JP-rT : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGTTTTGCAGGTGCCGGCTAGGCCTTCGGGTTTGTCGGCATGCTTCCAGATGCCTTTAAACGGGTGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 PILO-JP-rT : CATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTTCGGATCCGAAGTAATGGTTAAGAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 PILO-JP-rT : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGGGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 PILO-JP-rT : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 PILO-JP-rT : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCCCGTGCGGGTGGCGGTGCTCGCTGCCTCTTCGGGGGTGGTGGTGTCGTTCACCGGCGGGTGCTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 PILO-JP-rT : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCCGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (1240) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 PILO-JP-rT : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGTTTCGGCAGCTCGACCGGCGCTGAGAAGACGACCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACA : 1980 Int seq (77) * 2000 * 2020 * 2040 * 2060 TRU1 * 2080 * 2100 * 2120 * 2140 * 2160 PILO-JP-rT : GAATTCCCTAAATTAAAAGTGCCTAGATGGGCAAATGGGCAAGTGTTTTCTAAGCGAGTAGTCATACTCGCTAAATGTAGCCTCGGCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGC * 2180 TRU2 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 TRU3 * 2320 * 2340 PILO-JP-rT : CTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGTGCATTGGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCAT * 2360 * 2380 * 2400 * 2420 TRU4 * 2440 * 2460 * 2480 * 2500 * 2520 PILO-JP-rT : TCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATG * 2540 TRU5 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 TRU6 * 2680 * 2700 PILO-JP-rT : AGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGC : 2160 : 2340 : 2520 : 2700 Int seq (356) * 2720 * 2740 * 2760 * 2780 TRU6.7 * 2800 * 2820 * 2840 * 2860 * 2880 PILO-JP-rT : CATTCGGTCCGGCTGTGCCTGGCGCATTCGTGTGCCTGCGCACAGTCGGATCTTTTCTGATGAGCCTTCGGGTTTGTTGGATGCAGACTCGTCTGCCTAAGGTGGAGCGTTTCTCCTCTGCCATTCGGTCCGGCTGTgCCTGGCGCATTCGTGCGCCTACGCACAGTCATGCTTTCAGCA : 2880 * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 PILO-JP-rT : GGGTGCCTATCCGTCTGATGCTCTTTGTTTTGCTTGCCATCTTCGGATGGTCAGTCCATCTCGGGAGTGACGGGTTGTACTGTCTTCATAGATAGTGCTAGGCTTAATGAGTGGTGAGGCATACTGAGCTACGGCTCGGCCACCGCCCTGTTTGTTTCAATTTATGCCGTTTCACACTGT : 3060 5.8S (160) * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 PILO-JP-rT : TCAAGTGGTTCGGGTCATCCTGTTTGGCCTGTTCCATTGCCCGACATGCACCCGGTCCCGTACTGGACTGCATGTACAGTCGCCTGGCGGTGCCTTATCCCGGGCTCGACTGGTTAACCATACGTCGTTCGTCCGGGTGACCGAATGTTCGATGTGAGTACAACTCTGTGCGGTGGATCA : 3240 ITS2 (285) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 ITS2 * 3400 * 3420 PILO-JP-rT : CTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGCGAACTGCATACTGCTTTGAGCATCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTG : 3420 * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 PILO-JP-rT : CCAGCTGGCGTGATTTCCCCAATCTGACCATGTGTTGGTGGGGTGCCAGATCTATGGCGTTTCCCTAACCTATCCGGGCGTACCCATGTTGTGGCTGAAGGCCTTGGTGGGGATGTGGCAACGGAATCGTGGCTCAGTGGATTATTTATGTGCGCGTTCCGTTGTCCTATCATCATCTAT : 3600 28S (3881) * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 PILO-JP-rT : GGTTGATGCTGCGCATGGTGTGCGTCCGACGCCAACCTACGTATGGGCCGTGTGGCTCATTCTCCTGACCTCGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCAC * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 PILO-JP-rT : CGAAGCCTGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTGCTGCTCCACCCTAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAAAGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCG * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 PILO-JP-rT : GGTTGTTTGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAATTGAACTGCAAGCTTTGAGG * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 PILO-JP-rT : ATTCAGCTGGTGAGTGTGGTTTGGGCTTGGTCAAGTCGGTTGGACATTGAGGTCTGTGTAGCAGCAGGTCTCTGCCCCCGGGCAGGGATGCGCGATACACTTATCAAGTGTTGTGCGCCTCTTTGTTTATCGGGCCAGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCAC * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 PILO-JP-rT : TGCTGTCTGACTGCTGTGGTTAAACCGGACTTACAGAGCACCTGTGGCTTTGTATGGCCGGGAAGGCAGGTAGTTCGTTGGCTAGCTTGTGGTTTACCATAGGCGTTTGGCTTCCGAGCGTAATTCGCTGACCACGATTGTTCTGTGCAGTGTGTCGGAGACGGCGGCTTGTGGTGCGTG * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 PILO-JP-rT : CATGCGTGCCGGTTCTGCTGGCGTGTTCGGGTTTGGTTGCCATGTTGCTTGTGAATGCAAGCCTGGTGATGGCTCGGATTCGTTTAGTTGACGGTTGCGTGTGTGGTGCCGTTAAGGGCCAACAGTCTGTGGTGTAGTGGTAGATTATCCACCTGACCCGTCTTGAAACACGGACCAAGG * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 PILO-JP-rT : AGAGTAACATGTGCGCGAGTCATTGGGCGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGGCTTGGCTTGTCCAGGCTGAGGTGAGATCCCGTCGTTTTCATGCATGGTACCACCAAGCAACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAGTCTTCGGACCA * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 PILO-JP-rT : GTGCACCGTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 PILO-JP-rT : TCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAGTAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGG * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 PILO-JP-rT : CCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAACGCGACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCC * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 PILO-JP-rT : CTGAAAATGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGGGTCGGTAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGG * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 PILO-JP-rT : TGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATGGGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTCGGTACAGAGACGGGGTGCCTGACACTGTCAGGATTGCTGCTCGTACTGTTGCAAGGCAG * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 PILO-JP-rT : CCCCCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGTATTCCGGTCATGTGCTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCATCCATCCCCTGGAATC * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 PILO-JP-rT : GGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAACGCTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTA * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 PILO-JP-rT : AGGGAAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGGATCAGATACGTGCCGGTCAAGTTGGGGGCTGGTTTTTCTGTTGCTCTGTTCGCTTCGGTGGATGGAGTTGATGGGATTACTGGACTCTGACCAGGCCGGTCGTGGAC * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 PILO-JP-rT : GATCTCAGCTGTAGTGCTGCTTGGTTCGCGTCGTGCGTGGGAAACCGTGTGCGTCTGCGGCCTTGCTTCCTTCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGACCGGTTTTGACGCAATGTG * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 PILO-JP-rT : ATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCA * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 PILO-JP-rT : AGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGATCTCTCGAGATTTGGCGTCAGTGAAATACCACCACCGTCATTGTTTCTTTGCTTATTCAGTAAAGCGGGGTAC * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 PILO-JP-rT : CAATTTTGGTCCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTCATGTCAGATGAGCCGTGGCTTCGGCTGCGACGTTTGAGTTTGAATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGT * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 PILO-JP-rT : AACGCAGGTGTCCCAAGGTAAGCTCAGTCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGATCCTTTTGATTTTGATATTCGGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGG * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 PILO-JP-rT : GATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAG * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 PILO-JP-rT : TACAATGAAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : (7,7 TRU) ///-IGS-/// : 3780 : 3960 : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 : 7020 : 7200 : 7380 7543 ======================================= Hình PL3.7 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome (rTU) loài Paragonimus miyazakii của Nhật Bản (Pmiy-OkuST1-JP), thiếu IGS (6.932 bp; ITS1 chứa RU) 18S (1974) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Pmiy-OkuST : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACATACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Pmiy-OkuST : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGTGGTTTCGGCTGCTTCCGTTGTACTCTGTGATGACTCTGGATAACTTCACTGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTACGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Pmiy-OkuST : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Pmiy-OkuST : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTTTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Pmiy-OkuST : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGTTTTGCAGGTGCTGACTAGGCCTTCGGGTTTGTCGGCATGCTTCCGGATGCCTTTAAACGGGTGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Pmiy-OkuST : CATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTTCGGATCCGAAGTAATGGTTAAGAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Pmiy-OkuST : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGTGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Pmiy-OkuST : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Pmiy-OkuST : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCTCGTGCGGGTGGCGGTGCTCGCTGCCTCTTCGGGGGTGGTGGCGCCGTTCGCCGGCGGGTGCTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Pmiy-OkuST : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCTGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (634) 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Pmiy-OkuST : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGTTTCGGCAGCTCGACCGGCGCTGAGAAGACGACCAAATTTGAACATTTAGAAGTGAAAGTCGTGACAAAGTTTCCGTAGGTGAACCTGGAGAAGGATCATTAGAGAA * 2000 * 2020 * 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Pmiy-OkuST : TTGCCGAAATTCAAAATGCCTCGATGAGCAAATGGGCAAGTGTTCTCCACGGGTAGTCATACGCCTTAAATGCAGCTTCGGCTGCCTCAGGTAGAGCGTCTCTCCCCTGCCATTTGATTCAGCTGTGTCTGGCGAACTCCAGTGGCTGCATTCAGTTGAATCTTTTCCGACGAGCCTTCA * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 * 2320 * 2340 Pmiy-OkuST : GTTTTGTTGGATGCAACCTCATCTGCATCAGGTGGAGCGTCTTTCCTATGCCATTTAATCCAGCTGTACCTGGCGCACATGCGTGCCAATGTACAGTTATCCTTGCAGCAAGGCACCTACCTATCCGATGCTCTTTGAGTTGCTTGCCATTTTCGGATAGCGAATGCATCTCGGGAGTGA * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Pmiy-OkuST : CGGGTTGTACTGTGCTCATAGACAGCGCCAGGCTTAATGGGTGGTGAGGCATATTGAGCTACAGATCGGCCACCGCCATGTTTTGTTCCAATCTATGCCATTTCACACTGTTCAAGTGGTCCAGGTCGGCCTGTCTGAATTGGTCCATTGCCCGACATGCATCCGGTTCCGTACTGGACT : 1980 : 2160 : 2340 : 2520 5.8S (160) * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 Pmiy-OkuST : GCATGTACAGTCGACTGGCGGTGCCTGATCCCGGGCTTGACTGGCTAACCATACGTCGTTCTTCTGGCTGACTGGATGTTCCATGTGAGTATAGCTCTGTATGGTGGGTCACTTGGCTCGTGTGTTGATGAAGAGCGCAGCCAACTGTGTGAATTAATGTGAAATGCATACTGCTTTGAA : 2700 ITS2 (283) * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Pmiy-OkuST : CATCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTGCCAGCTGGCGTGATTTCCCCAACCTGGCCTCGTGGCTGTGGGGTGCCAGATCTGTGGCGTTTCCCTAAC : 2880 28S (3881) * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Pmiy-OkuST : ATATCCGGGCGTACCCATGTTGTGGCTGAAAGCTTTGATGGGGATGTGGCAACGGAATCGTGGCTCAGTGATTGATTTGTGCGCGTTCCGCTATCCTATCATCGTCTATGGTTGATGTTGCGCGTGGTGTGTGCCCGATGCTGACCTATGTATGTGCCATGTGGCTCATTCTCCTGACCT * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Pmiy-OkuST : CGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTGCTGCTCCACCC * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 Pmiy-OkuST : TAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAAAGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCGGGTTGTTTGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCACGAGTCCGAT * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Pmiy-OkuST : AGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGCAAGCTTTGGGGATTCAGCTGGTGAGTGTGGTTTGAGCTTGGTCAAGTCGGTTGGACATTGGGGTCTGTGTAGCAGCAGGTCT * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Pmiy-OkuST : CTGCCCTTGGGCAGGGATGCGCGATGCACTTATCAGGTGTTGTGCGCCTCTTTGTTTATCGGGCCAATTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCACTGCTGTCTGACTACTGTGGTTAAACCGGACTTACAGAGCACTTGTGGTTTTGTATGGCCGGGAAGGCAGGT * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Pmiy-OkuST : AGCTCGTTGGCTAGCTTGTGGTTTACTGCAGGCGTTTGGTTTTCGAGTGTAATTAGCTGACCACGATTGTTCTGTGCAGTGTGTCGGAGACGGCGGCTTGTGGTACTTGCGTGCGTGCCGGTCCTGCTGGCGTGTTCGGGTTTGGTTGCCATGTTGCTTGTGAATGCAGGCCTGGTGATA * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Pmiy-OkuST : GCTCGGATTCGTTCGGTTGGCGGTTGCGTGCGTGGTGCCGTTAAGGGCCAACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGGCCCGGCTTG * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Pmiy-OkuST : TCCAGGCTGAGGTGAGATCCTGTCGTTTTCACGCATGGTACCACCAAGCAACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAATCTTCGGACCAGTGCACCGTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGT * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Pmiy-OkuST : TGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAATAAACAGTTTTATCCGGTAAAGCGAATGA * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Pmiy-OkuST : TTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAACGCGACGCT * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Pmiy-OkuST : CATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGAGTCGGTAATGTGTG * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Pmiy-OkuST : GTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATG * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Pmiy-OkuST : GGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTCGGTACAGAGACGGGGTGCCTGACATTGTCAGGATTGCTGCTCGTATTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGTATTCCGGTCTT * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Pmiy-OkuST : GTGCTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCATCCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAACGCTCTTGT * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Pmiy-OkuST : CGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTG * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Pmiy-OkuST : GGATCAGATACGTGCCGGTCAAGTTGGGGGCTGGTTTTTCTGTCACTCTGTTCGCTTCGGTGGATGGGGTCGATGGGATTACTGGACTCTGACCAGGCCGGTCGTGGACGATCTCAGCTGTAGTGCTGCTTGGTTCGCATCGCGCGTGGGAAACTGTGTGCGTTTGTGGCCTTGCTTCCT * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Pmiy-OkuST : TCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGACCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGGGAGTAACT * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Pmiy-OkuST : ATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGA * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Pmiy-OkuST : GGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGGTCCTTCGGGGTTTGGCGTCAGTGAAATACCACCACCGTCATTGTTTCTTTGCTTATTCAGTAAAGCGGGGTGCCAATTTTGGTCCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTCATGTCAGA * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Pmiy-OkuST : TGAGCCGTGGCTTCGGCTGCGGTGTTTGAGTTTGAATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTAAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACT * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Pmiy-OkuST : TGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGATCCTTTTGATTTTGATATTCGGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCT * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Pmiy-OkuST : AGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGG * 6860 * 6880 * 6900 * 6920 * Pmiy-OkuST : TTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 6932 bp (0 TRU) ///-IGS-/// : 3060 : 3240 : 3420 : 3600 : 3780 : 3960 : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 * Hình PL3.8 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome (rTU) loài Paragonimus westermani (2n) của Ấn Độ (Pwes-Megha(2n)-IN), thiếu IGS (8.616 bp; ITS1 chứa 15,7 TRU) 18S (1977) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Pwes(2n)-I : AACATGGGTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACATACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Pwes(2n)-I : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGTGGCCTTGGCTGCATCCGTTGTACTCTGTGATGACTCTGGATAACTTCACTGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTGCGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Pwes(2n)-I : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Pwes(2n)-I : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTGTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Pwes(2n)-I : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGGTTCACAGGTGCTGACTAGGCCTTCGGGTTTGTCGGCATGCTCCCACATGCCTTTAAACGGGTGTCTgGGGCGGACTGCACgTTTACTTTGAACAAATTTGAGTGCTCAAAGCAgGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Pwes(2n)-I : CATGGAATAATGGAATAGGACTTCgGTTCTATTTTGTTGGTTTTCGGATCCgAAGTAATGGTTAAGAGGGACAGACGGGGTCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGgATCGCCGcCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Pwes(2n)-I : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGTGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Pwes(2n)-I : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Pwes(2n)-I : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCCCGTGCGGGTGGCGGTGCTCGCTGCCTCTTCGGGGGTAGTGGTGTCGTTCGCCGGCGGGTACTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Pwes(2n)-I : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCCGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (2313) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Pwes(2n)-I : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGCTTCGGCAGCTCGACCGGCGCTGACAAGACGACCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACA : 1980 Int seq (77) * 2000 * 2020 * 2040 * 2060 TRU1 * 2080 * 2100 * 2120 * 2140 * 2160 Pwes(2n)-I : GAATTCCCAAATCTAAAGGTGCCTAAATGAGCACAAGGGCAAGTGTTTTCTCAACGAGCAGTCATGCTCGTCAATTGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCC : 2160 * 2180TRU2 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300TRU3 * 2320 * 2340 Pwes(2n)-I : TTCGGGTTTGTTGGATGCAGCCTCG TCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTC GTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTG : 2340 * 2360 * 2380 * 2400 * 2420TRU4 * 2440 * 2460 2480 * 2500 * 2520 * Pwes(2n)-I : ATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCC : 2520 * 2540TRU5 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660TRU6 * 2680 * 2700 Pwes(2n)-I : TTCGGGTTTGTTGGATGCAGCCTCG TCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTC GTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTG : 2700 * 2720 * 2740 * 2760 * 2780TRU7 * 2800 * 2820 2840 * 2860 * 2880 * Pwes(2n)-I : ATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCC : 2880 * 2900TRU8 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020TRU9 * 3040 * 3060 Pwes(2n)-I : TTCGGGTTTGTTGGATGCAGCCTCG TCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTC GTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTG : 3060 * 3080 * 3100 * 3120 * 3140TRU10 * 3160 * 3180 3200 * 3220 * 3240 * Pwes(2n)-I : ATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCC : 3240 * 3260TRU11 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380TRU12 * 3400 * 3420 Pwes(2n)-I : TTCGGGTTTGTTGGATGCAGCCTCG TCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTC GTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTG : 3420 3440 3560 * * 3460 * 3480 * 3500TRU13 * 3520 * 3540 * 3580 * 3600 * Pwes(2n)-I : ATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCC : 3600 * 3620TRU14 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740TRU15 * 3760 * 3780 Pwes(2n)-I : TTCGGGTTTGTTGGATGCAGCCTCG TCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTC GTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTG : 3780 Int seq (350) * 3800 3920 * 3820 * 3840 * 3860TRU15.7 * 3880 * 3900 * 3940 * 3960 * Pwes(2n)-I : ATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTCGAATCTTTTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGATTCGGCTGTGCCTGGCGCACTCGTGTGCCTACGTACAGTTATGCTTGCAGCAGGGTGCC : 3960 * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Pwes(2n)-I : TACCTGTCTGATGCCCTTCGTTTTGCTTGCCATCTTTTGATGGTCAGTCCATCTCGTGGGTGACGGGTTGTACTGTCTTCATGGACAGTGCTAGGCTTAATGAGTGGTACGGCATATTGAGCTACGGCTCGGCCACCGCCCTGTTTGTTATAATTTATGCCATTTTACACTGTTCAAGTG : 4140 5.8S (160) * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Pwes(2n)-I : GTTCAGGTCAGCTCGTCTGGGCTGTTCCATTGCCCGACATGCACCCGGTCTCGTACTGGACTGCATGTACAGTCGCCTGGCGGTGCCTTATCCCGGGCTAGACTGGTTAACCATACGTCGTTCGTCTGGGTGACTGGATGTTCGATGTGAGTACAACTCTGTGCGGTGGATCACTCGGCT : 4320 ITS2 (285) * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Pwes(2n)-I : CGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGCGAACTGCATACTGCTTTGAACATCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTGCCAGCTG : 4500 * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Pwes(2n)-I : GCGTGATCTCCCCAATCTGGTCTTGTGCCTGTGGGGTGCCAGATCTATGGCGTTTCCCTAACATACTCGGGCGCACCCACGTTGCGGCTGAAAGCCTTGACGGGGATGTGGCGACGGAATCGTGGCTCAGTAAATGATTTATGTGCGCGTTCCGCTGTCCTGTCTTCATCTGTGGTTCAT : 4680 28S (3881) * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Pwes(2n)-I : GTTGCGCGTGGTCTGCGTTTGATGCTGACCTATGTATGTGCCATGTGGCTCATTCTCCTGACCTCGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAGAGAAACTAACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCC * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Pwes(2n)-I : TGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTGCTGCTCCACCCTAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAAAGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCGGGTTGTT * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Pwes(2n)-I : TGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGCAAGCTCTGGGAATTCAGC * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Pwes(2n)-I : TGGTGAATGTGGTTTGGGCTTGGTCAAGTCGGTTGGACATTGAGGTCTGCGTACCGGCAGGTCTTTACCCTCGGGTAAGGATGCGCGATACACTTATCAAGTGTTGTGCGCCTCTTTGTTTATCGGGCCAATTCGCCAGTGCACTTTCTCGGAGTGTTCACCACGACCGGCACTGCTGTC * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Pwes(2n)-I : TGATTGCTGTGGTTAAACCGGACTTACAGGGCTCTTGTGGCGTTGTATGGCCGGGAAGGCAGGTAGCTCGTTGGCTAGCTTGCGGTTTACCGCTGGCGTTCGGTCTTCGAGTGTAAATAGCTGACCACGATATTTCTGTGCAGTGTGTCGGAGACGGCGGCTTTTGGTACGTGCGTGCGT * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Pwes(2n)-I : GCTGGTTCTGCTGGCGTGTCCGGGTTTGGTTGCCATGTTGCTTGTGAATGCAGGCCTGGCAATGGTTCGGATTCGTTTGGTGGGCGGTTGCGTATGTGGTGCCGTTAAGGGCCAACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAA * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Pwes(2n)-I : CATGTGCGCGAGTCATTGGGCGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGGCTTGACTTGTTCAGGCTGAGGTGAGATCCTGTCGTTTTCACGCATGGTGCCACCAAGCAACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAATCTTCGGACCAGTGCACC * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Pwes(2n)-I : GTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Pwes(2n)-I : GAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAGTAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGGCCATTTT * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Pwes(2n)-I : TGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAACGCGACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAA * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Pwes(2n)-I : TGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGGGTCGGTAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGGTGCAGAT * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Pwes(2n)-I : CTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATGGGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTCGGTACAGAGACGGGGTGCCTGACATTGTCAGGATTGCTGCTCGTATTGTTGCAAGGCAGCCCCCTG * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Pwes(2n)-I : TAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGTATTCCGGTCTTGTGCTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCATCCATCCCCTGGAATCGGGTTGT * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Pwes(2n)-I : CCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAACGCTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTAAGGGAAG * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 Pwes(2n)-I : TCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGGATCAGATACGTGCCGGTCAAGTTGGGGGCTGGTTTTTCTGTCACTCTGTTCGCTTCGGTGGATGGGGTCGATGGGATTACTGGACTCTGACCAGGCCGGTCGTGGACGATCTCA * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 Pwes(2n)-I : GCTGTAGTGCTGCTTGGTTCGCATCGCGCGTGGGAAACTGTGTGCGTTTGTGGCCTTGCTTCCTTCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGACCGGTTTTGACGCAATGTGATTTCTG * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * 7740 Pwes(2n)-I : CCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAAC * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * 7920 Pwes(2n)-I : GGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGGTCCTTCGGGGTTTGGCGTCAGTGAAATACCACCACCGTCATTGTTTCTTTGCTTATTCAGTAAAGCGGGGTGCCAATTTT * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * 8100 Pwes(2n)-I : GGTCCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTCATGTCAGATGAGCCGTGGCTTCGGCTGCGGTGTTTGAGTTTGAATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGTAACGCAG * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * 8280 Pwes(2n)-I : GTGTCCCAAGGTAAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGATCCTTTTGATTTTGATATTCGGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACT * 8300 * 8320 * 8340 * 8360 * 8380 * 8400 * 8420 * 8440 * 8460 Pwes(2n)-I : GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATG * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * Pwes(2n)-I : AAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : (15,7 TRU) ///-IGS-/// : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 : 7020 : 7200 : 7380 : 7560 : 7740 : 7920 : 8100 : 8280 : 8460 8616 bp ======================================= Hình PL3.9 Trình bày diễn giải gen vùng giao gen của đơn vị mã hóa ribosome (rTU) lồi Paragonimus westermani (3n) của Hàn Q́c (Pwes-Bogil(3n)-KR), thiếu IGS (7.292 bp; ITS1 chứa 4,7 TRU) 18S (1977) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Pwes-KR(3n : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACATACCTCAAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTTAGATCGTACCTACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGC * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Pwes-KR(3n : CCTGACCCGCGAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTTGTGGCTCCGGCTGCATCCGTTGTACTCTGTGATGACTCTGGATAACTTCAATGATCGCGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTACGATGGTAGGTGACCTGCCTA * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Pwes-KR(3n : CCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCACGCAAATTACCCAATCCCGGCACGGGGAGGTAGTGACGAAAAATACGAATACGGGACTCAACAGAGGCTCCGTAATTCGAATGAGTACA * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 Pwes-KR(3n : ATTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACGTGCCGTCGCTCACTTTCTCGGTTGGCTGTTAAGGCCCTGGGTGTG * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Pwes-KR(3n : GTGGGTCGGTGTCGTGGTTGTGTGGTCTTTCTGCCGTGTCTGGTTCACAGGTGCTGACTAGGCCTTCGGGTTTGTCGGCATGCTTCCAGATGCCTTTAAACGGGTGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCTTGTGCCTGAAAATTCTTG * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Pwes-KR(3n : CATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTTCGGATCCGAAGTAATGGTTAAGAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCTGGAGCGAA * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Pwes-KR(3n : AGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGCGGTGGTTCTTTTATTGTCCCCGCGGGCAGTCCCCGGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Pwes-KR(3n : ACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGGAAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGAAAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTTTG * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Pwes-KR(3n : GCCTGCTAAATAGTATGCCTGTCCTCTGTGCCCGTGCGGGTGGCGGTGCTCGCTGCCTCTTCGGGGGTAGTGGTGCCGTTCGCCGGCGGGTACTGCGCAGGTGTTTACTTCTTAGAGGGACAAGCGGCGTGACAGTCGCACGAAAATGAGCAATAACAGGTCTGTGATGCCCTTAGATGT * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Pwes-KR(3n : CCGGGGCCGCACGTGCGCTACAATGACGGTTTCAGCGAGTCTGGGAACCTGGCCCGAAAGGGTTGGGCAAACTGAGTCATCACCGTCGTGACTGGGATCGGGGCTTGCAATTGTTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGATTACGTCCCTGCCCT : 180 : 360 : 540 : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 ITS1 (989) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Pwes-KR(3n : TTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGCTCCGGCAGCTCGACCGGCGCTGAGAAGACGACCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACA : 1980 Int seq (73) * 2000 * 2020 * 2040 * TRU1 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Pwes-KR(3n : GAATTCCCAATTCTGAAACTGCCTCAATGAGCACAAAGGCGTGTTCTAAACGAGCAGTCATGCTCGTCAAACGCAGACTCGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGTTTCGGCTGTGCCTGGCACACTCGTGTGCCTACGTACAGTCGGATCTTCTCCGATGAGCCTTCG : 2160 * TRU2 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * TRU3 2300 * 2320 * 2340 Pwes-KR(3n : GGTTTGTTGGATGCAGCCTTGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGTTTCGGCTGTGCCTGGCACACTCGTGTGCCTACGTACAGTCGGATCTTCTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTTGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGTTTC : 2340 * 2360 * 2380 * 2400 * TRU4 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Pwes-KR(3n : GGCTGTGCCTGGCACACTCGTGTGCCTACGTACAGTCGGATCTTCTCCGATGAGCCTTCGGGTTTGTTGGATGCAGCCTTGTCTGCCTCGGGTGGAGCGTTCTCCTCTGCCATGTGTTTCGGCTGTGCCTGGCACACTCGTGTGCCTACGTACAGTCGGATCTTCTCCGATGAGCCTTCG : 2520 Int seq (350) *TRU4.72540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 Pwes-KR(3n : GGTTTGTTGGATGCAGCCTTGTCTGCCTCAGGTGGAGCGTTCTCCTCTGCCATGTGTTTCGGCTGTGCCTGGCACACTCGTGTGCCTACGTACAGTTATACTTGCAGCAGGGTGCCTACCTGTCTGATGCTCTACGTTTTGCTTGCCATTTTCGGATGGTCAGTCCATCTCGTGGGTGAC : 2700 * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Pwes-KR(3n : GGGTTGTGCTGTCTTCACCGACAGTGCTAGGCTTAATGAGTGGTATGGCATATTGAGCTACGGCTCGGCCACCGCCCTGTTTGTTTCAATTTATGCCATTTTACACTGTTCAAGTGGTTCAGGTCAGCTCGTCTGAGCTGTTCCACTGCCCGACATGCACCCGGTCTCGTGCTGGACTGC : 2880 5.8S (160) * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Pwes-KR(3n : ATGTACAGTCGCCTGGCGGTGCCTTATCCCGGGCTAGACTGGTTAACCATACATCGTTCGTCTGGGTGACTGGATGTTCGATGTGAGTACAACTCTGTGCGGTGGATCACTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGCGAACTGCATACTGCTTTGAACA : 3060 ITS2 (285) * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Pwes-KR(3n : TCGACATCTTGAACGCATATTGCGGCCACGGGTTAGCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCGCGACGCCCAAAAAGTCGCGGCTTGGGTTTTGCCAGCTGGCGTGATCTCCCCAATCTGGTCTTGTGCCTGTGGGGTGCCAGATCTGTGGCGTTTCCCTAACAT : 3240 28S (3881) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 Pwes-KR(3n : ACTCGGGCGCACCCACGTTGCGGCTGAAAGCCTTGACGGGGATGTGGCAACGGAATCGTGGCTCAGTGAATGATTTATGTGCGCGTTCCGCTGTCCTGTCTTCATCTGTGGTTTATGTTGCGCGTGGTCTGCTTTCGATGCTGACCTACGTATGTGCCATGTGGTTCATTCTCCTGACCT * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Pwes-KR(3n : CGGATCAGACGTGAGTACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAGAGAAACTCACAAGGATTCCCTGAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGTCATTTGGTCACTAGGCAATGTGGTGTTCAGGTCGTTCCATAGAGGTGCTGCTCCACCC * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Pwes-KR(3n : TAAGTCCAGCAATGAGTACGGTGGTACGGACATGGCCCACAGAGGGTGAAAGGCCCGTGGGGGTGGAGATCAGGCAGGCCAGTGCCTCTCTGGGTAGACCTTGGAGTCGGGTTGTTTGTGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTTGCACGAGTCCGAT * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Pwes-KR(3n : AGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGCAAGCTTCGGGAATTCAGCTGGTGAGTGTGGTTTGGGCTTGGTCAAGTCGGTTGGACATTGAGGTCTGTGTAGCGGCAGGTCT * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Pwes-KR(3n : TTACTTTCGGGTAAGGATGCGCGATACACTTATCAAGTGTTGTGCGCCTCTTTGTTTATCGGGCCAATTTGCCAGTGCACTTTCTCGGAGTGTTCACCACGACCGGCACTGCTGTCTGATTGCTGTGGTTAAACCGGACTTACAGGGCTCTTGTGGCGTTGTATGGCCGGGAAGGCAGGT * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Pwes-KR(3n : AGCTCGTTGGCTAGCTTGCGGTTTACCGCTGGCGTTTGGTTTTCGAGTGTAAATAGCTGACCACGATATTTCTGTGCAGTGTGTCGGAGACGGCGGCTTGTGGTACGTGCGTGCGTGCCGGTTCTGCTGGCGTGTCCGGGTTTGGTTGTCATGTTGCTTGTGAATGCAGGCCTGGCAATG * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Pwes-KR(3n : GTTCGGATTCGTTTGGTGGGCGGTTGCGTATGTGGTGCCGTTGAGGGCCAACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGTGTAACGAAACCCACAGGCGCAGTGAAAGTAAAGGCTTGGCTTG * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Pwes-KR(3n : TCCAGGCTGAGGTGAGATCCTGTCGTTTTCACGCATGGTGCCACCAAGCAACGAGCGGCAGGCGCATCACCGGCCCGTCCCATGACGTAGACACATAATCTTCGGACCAGTGCACCGTCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGT * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Pwes-KR(3n : TGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAAACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGTTCGGAGAAGTAAACAGTTTTATCCGGTAAAGCGAATGA * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Pwes-KR(3n : TTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCTGAACTCGCTTGAGCGGAGTTGGGCGTTGAATGTGAGTGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCCGGTTAAGGTGCCTAACGCGACGCT * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Pwes-KR(3n : CATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCTATACCGGACCGTTACTGCAAGCTGGGTCGGTAATGTGTG * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Pwes-KR(3n : GTGTGAGGGCAAGCCGCACGACTCAGGCACGGAGTGGTGGTAACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTGAGCTTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATG * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Pwes-KR(3n : GGAACAGCAGTTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGATAACTCGGTACAGAGACGGGGTGCCTGACATTGTCAGGATTGCTGCTCGTATTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGTATTCCGGTCTT * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Pwes-KR(3n : GTGCTGGTTTGCATCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGTGCATCCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACCCCTCTTGTGGTGTCCGGAACGCTCTTGT * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Pwes-KR(3n : CGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCATTTGAACAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTG * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Pwes-KR(3n : GAATCAGATACGTGCCGGTCAAGTTGGGGGCTGGTTTTTCTGTTACTCTGTTCGCTTCGGTGGATGGGGTCGATGGGATTACTGGACTCTGACCAGGCCGGTCGTGGACGATCTCAGCTGTAGTGCTGCTTGGTTCGCATCGCGCGTGGGAAACCGTGTGCGTTTGTGGCCTTGCTTCCT * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 Pwes-KR(3n : TCGTTTGGCGTTAAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGACCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAGCGCGGGTAAACGGCGGGAGTAACT * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Pwes-KR(3n : ATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGA * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Pwes-KR(3n : GGAGACATGACGGGTGTAGAATAAGTGGGAGCCAGGTCCTTCGGGGTTTGGCGTCAGTGAAATACCACCACCGTCATTGTTTCTTTGCTTATTCAGTAAAGCGGGGTGCCAATTTTGGTCCTAAGCACATGCTGGCATGCGTGGCTTTGCTGCGGGTGTCGGTATCCTGGTCATGTCAGA * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Pwes-KR(3n : TGAGCCGTGGCTTCGGCTGCGGTGTTTGAGTTTGAATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTAAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACT * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Pwes-KR(3n : TGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGTGGCCTATCGATCCTTTTGATTTTGATATTCGGAGTTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCT * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Pwes-KR(3n : AGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAAACAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGG * 7220 * 7240 * 7260 * 7280 * Pwes-KR(3n : TTCAGACATTTGGTATATGTGCCTGGTCGAAAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 7292 bp (7,7 TRU) ///-IGS-/// ======================================= : 3420 : 3600 : 3780 : 3960 : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 : 6480 : 6660 : 6840 : 7020 : 7200 PHỤ LỤC II PL2.1 Phương pháp tách chiêt DNA tổng số từ mẫu nghiên cứu DNA tổng số từ SLP trưởng thành Paragonimus spp nguyên từng mẫu riêng biệt tách chiết bằng bộ sinh phẩm DNeasy Blood and Tisue kit (Qiagen Inc Mỹ) hoặc GeneJET™ Genomic DNA Purification Kit (Thermo Scientific Inc., MA, USA) theo hướng dẫn nhà sản x́t DNA tởng số thu nhận với dung tích khoảng 100 µL/mẫu, hàm lượng DNA 50 ng/µL Mơ tả cụ thể tách chiết DNA tổng số sinh phẩm DNeasy Blood and Tissue kit (Qiagen Inc Mỹ) : - Mỗi mẫu sán ruột nhỏ (1 con) nghiền 180 µL dung dịch Tissue Lysis buffer (ATL) bở sung 20 µL Proteinase-K (100mg/ml) ủ 56 oC/2 giờ cho đến mô phân giải hồn tồn - Tiếp tục bở sung 200 µL dung dịch AL lắc ủ 56oC/30 phút - Thêm 200 µL Ethanol (100%), trộn đều, sau chuyển tồn bộ hỗn dịch sang cột có màng lọc ly tâm 10.000 vòng/phút phút, loại bỏ phần dịch phía dưới cột lọc - Thêm 500 µL dung dịch Washing buffer (AW1) vào cột lọc, ly tâm 13.000 vòng/phút phút, bỏ dịch ly tâm bên dưới cột lọc - Tiếp tục thêm 500 µL dung dịch Washing buffer (AW2) vào cột lọc để rửa DNA, ly tâm 13.000 vòng/phút phút, bỏ dịch bên dưới, ly tâm lại 13.0 vòng/phút phút để làm khô màng - Chuyển cột sang Eppendorf mới, thêm 60 µL dung dịch Elution buffer (EL), để nhiệt độ phịng khoảng 2–3 phút, sau ly tâm 13.000 vòng/phút phút Bỏ cột, thu dịch bên dưới DNA tổng số, ghi ký hiệu mẫu bảo quản mẫu DNA – 20oC cho đến sử dụng - Kiểm tra hàm lượng DNA tổng số bằng cách đo hấp phụ quang học (OD, optical density) máy đo quang phở kế NanoDrop®ND-1000 UV-Vis Spectrophotometer (Thermo Scientific Inc.) DNA tổng số pha thành nồng độ 50 ng/µL, sử dụng 1–2 µL cho phản ứng PCR có dung tích 50 µL PL2.2 Phương pháp thiêt kê mồi chung và mồi đặc hiệu mtDNA Các mồi chung (hay gọi mồi ty thể chung cho sán dẹt, universal- platyhelminth primers) sử dụng cho đề tài thu toàn bộ mtDNA SLP từ thiết kế liệt kê công bố trước (Le et al., 2002; 2016; 2019) hoặc/và cải tiến bổ sung thêm để thực PCR dài (long-PCR/ L-PCR) với mục tiêu thu nhận đoạn mtDNA có độ dài cao nhất có thể, 4–6 kb hoặc dài (Bảng PL2.1) Một số cặp mồi đặc hiệu mtDNA từng loài Paragonimus spp thiết kế để thu nhận sản phẩm PCR ngắn (1,5–3 kb) từ khuôn DNA tổng số hoặc/và từ khuôn sản phẩm LPCR thu nhận primer sử dụng để giải trình tự Chuỗi mồi thiết kế bằng chương trình Primer3Plus (https://www.bioinformatics.nl/cgi-bin/primer3plus/) Mồi chung sử dụng để thu nhận mtDNA của P heterotremus P ohirai: Sử dụng cặp mồi chung: i) URNLF/URNSR thu vùng gen rrnL-rrnS (~1000 bp); ii) TRECOBF/TRECOBR thu đoạn gen cob (~650 bp); iii) PNAD1F/JNAD1R thu đoạn gen nad1 (~400 bp); iv) JB3F/JB4.5R thu đoạn gen cox1 (444 bp) giải trình tự Các chuỗi sử dụng để thẩm định loài theo danh pháp tiếp tục thiết kế mồi đặc hiệu khởi điểm cho phản ứng PCR/LPCR Một số gen có mức độ bảo tồn cao 16S (rrnL) hay 12S (rrnS) hay chuỗi nucleotide tRNAGly định vị sau gen nad5 sử dụng thiết kế mồi lần lượt UNI16F UNI16R (trên rrnL), UNI12F UNI12R (trên rrnS), GLYF GLYR (trên tRNAGly) cho phản ứng L-PCR qua vùng mã hóa khơng mã hóa mtDNA (Bảng PL2.1) Bảng PL2.1 liệt kê tất mồi chung mồi đặc hiệu từng loài thiết kế sử dụng để thu sản phẩm PCR/LPCR có kích thước khác mtDNA P heterotremus P ohirai Trên sở trình tự nucleotide chuỗi thu nhận, tiếp tục thiết kế mồi giải trình tự bằng phương pháp kế tiếp lồng vào nhau, hay gọi phương pháp “lao mồi” trực tiếp từ sản phẩm hoặc/và sau tách dịng Giải trình tự thực sở dịch vụ: Macrogen (Hàn Quốc) (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) PL2.3 Phương pháp thiêt kê mời giải trình tự đơn vị mã hóa ribosome Bảng PL2.3 liệt kê tất mồi sử dụng để thu sản phẩm PCR/LPCR kích thước khác rTU chủng/5 loài P heterotremus, P ohirai, P iloktsuenensis, P miyazakii loài P westermani (2 chủng) Trên sở trình tự nucleotide chuỗi thu nhận, tiếp tục thiết kế mồi giải trình tự bằng phương pháp kế tiếp lồng vào nhau, hay cịn gọi phương pháp “lao mời” trực tiếp từ sản phẩm hoặc/và sau tách dòng Giải trình tự thực sở dịch vụ: Macrogen (Hàn Quốc) (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) Mồi sử dụng để thu nhận rTU chủng/5 loài P heterotremus, P ohirai, P iloktsuenensis, P miyazakii loài P westermani (2 chủng) (Bảng PL2.3) kế thừa từ nghiên cứu trước thiết kế bổ sung dựa một số cơng bố rTU có Ngân hàng gen hoặc liệu công bố có (Le et al., 2017; 2020b) hoặc sưu tầm để kết hợp sử dụng (Olson et al., 2003; Tkach et al., 2016) PCR/LPCR áp dụng bằng cách phối hợp cặp mồi khác lệch để thu phần DNA (phân đoạn) dài ngắn khác Toàn bộ đơn vị rTU (5’18S-ITS1-5.8S-ITS2-28S-IGS 3’) sán lá, biết cho đến có độ dài khoảng từ 7–8 cho đến 9–10 kb, tùy thuộc lồi Phần mã hóa (coding region), tạm ký hiệu rTU* tính từ đầu 5’ gen 18S cho đến hết đầu 3’ gen 28S, độ dài thường khoảng 6–7 hoặc kb, dài ngắn khác tùy loài Paragonimus Các gen 18S, 5.8S, 28S rRNA có độ dài cố định lồi một chi, thậm chí một họ Tuy nhiên, vùng ITS-1 (giữa gen 18S gen 5.8S) ITS-2 (giữa gen 5.8S gen 28S) vùng lề IGS, một số lồi có nhiều cấu trúc lặp liền kề có số lượng khác nhau, nên tởng kích thước sẽ khác Có một số mẫu thu giải trình tự tồn bộ phần mã hóa rTU*, khơng thu vùng lề (IGS) PL2.4 Thành phần phản ứng, thực PCR và kiểm tra sản phẩm Phản ứng PCR điều chỉnh thành phần chu trình nhiệt phù hợp để thu sản phẩm PCR tốt nhất thu nhận phân đoạn mtDNA rTU Chúng giới thiệu phản ứng có hiệu suất tốt nhất thực Phản ứng có tởng thể tích 50 µL, gồm: 25 µL PCR mastermix (Thermo Fisher Scientific, MA, USA), µL loại mồi (10 pmol/µL), µL khn DNA (25–50 ng/µL), µL DMSO (dimethyl sulfoxide) 17 µL nước khử ion DEPC, thực máy MJ PTC-100 (USA) Chu trình nhiệt gồm: chu kỳ 95oC/5 phút, 35 chu kỳ [94 oC/1 phút, 52oC/1 phút; 72oC/2–6 phút], chu kỳ cuối 72oC/10 phút Phản ứng L-PCR thực với enzyme Phusion DNA polymerase (Thermo Fisher Scientific Inc.), sản phẩm thu có độ dài 4–6 kb với chất lượng tốt Sản phẩm PCR/LPCR thu được điện di kiểm tra thạch agarose 1% nhuộm ethidium bromide, so sánh độ dài với thị DNA Lamda (HindIII) Sản phẩm PCR đơn băng tinh trực tiếp bằng bộ sinh phẩm GeneJET PCR Purification Kit (Thermo Scientific Inc.) Với sản phẩm đa băng, băng DNA với kích thước dự tính phân lập từ thạch agarose bằng bộ sinh phẩm GeneJET Gel Extraction Kit (Thermo Scientific Inc.) Các sản phẩm PCR tinh bằng bộ sinh phẩm GeneJET™ PCR Purification Kit gửi giải trình tự sở dịch vụ giới thiệu PL2.5 Phương pháp thu nhận DNA ribosome loài sán phởi L-PCR áp dụng thu nhận tồn bộ hoặc một phần ba (1/3) đến một nửa (½) vùng chép rTU (độ dài khoảng kb đến 4–5 kb, hoặc đến kb cho sản phẩm) với enzyme bộ sinh phẩm Phusion (Thermo Fisher Scientific) Các sản phẩm có kích thước dự tính có chất lượng tốt (rõ, đậm thạch agarose) tiếp tục sử dụng giải trình tự trực tiếp từ hai đầu biên hoặc/và làm khuôn cho PCR/LPCR mới bên để có sản phẩm tiếp tục giải trình tự Phần lớn sản phẩm PCR giải trình tự trực tiếp sau tinh sử dụng bộ sinh phẩm GeneJET PCR Purification Kit (Thermo Scientific Inc., MA, USA) theo hướng dẫn nhà sản xuất Chuỗi nucleotide từng phân đoạn sau biên tập (editing) bằng chương trình ChromasPro, lồng nối liên tiếp với để thu nhận hồn tồn trình tự rTU lồi Sử dụng chuỗi nucleotide biên tập cuối để xác định gen, phân tích cấu trúc, xếp gen, vùng biên, đặc điểm chuỗi nucleotide nội gen vùng giao gen Các chương trình ChromasPro, BioEdit, GENEDOC2.7 sử dụng để thu nhận, xử lý, phân tích chuỗi nucleotide, đặc điểm gen ribosome (18S, 5.8S, 28S) vùng giao gen gồm ITS-1 ITS-2 vùng lề IGS PL2.6 Phương pháp tách dòng, thu DNA plasmid tái tở hợp Sản phẩm PCR hoặc giải trình tự trực tiếp, nếu có chất lượng tốt hàm lượng đủ theo yêu cầu Một số sản phẩm tách dòng sử dụng vector TA cloning thương mại pCR2.1TOPO (Invitrogen) hoặc TOPO® XL PCR Cloning Kit (Invitrogen Inc) (nếu sản phẩm PCR kb, cho đến kb), chọn lọc plasmid tái tổ hợp để giải trình tự DNA plasmid tái tở hợp tách chiết với bộ hoá chất GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific Inc.) Nối sản phẩm PCR vào plasmid vector: Do sản phẩm PCR (sử dụng enzyme Taq DNA polymerase) thông thường gắn thêm Adenine (A) đầu 3’, nên nối với vector có đầu lồi Thymine (T) hãng sinh phẩm thiết kế từ trước, hai nucleotide sẽ gắn nối bổ sung cho nhờ enzyme nối T4-DNA ligase hoặc enzyme topoisomerase Vị trí để nối sản phẩm PCR vào bố trí chuỗi gen lacZ, gen mã hóa sản xuất enzyme β-galactosidase, có tác dụng xúc tác thủy phân một loại hóa chất có tên gọi X-gal để tạo nên dẫn chất có màu xanh Vi khuẩn không mang plasmid tái tổ hợp sẽ tạo nên khuẩn lạc màu xanh Khi sản phẩm nối vào vị trí nối thành cơng, đoạn DNA ngoại lai làm bất hoạt gen lacZ không cho gen sản xuất enzyme β-galactosidase Do vậy, vi khuẩn mang plasmid tái tở hợp sẽ khơng có enzyme β-galactosidase phân giải chất X-gal tạo màu xanh, nên khuẩn lạc sẽ mang màu trắng nuôi môi trường có chứa X-gal Chủn nạp ni cấy thạch: Tế bào sử dụng để chuyển nạp tế bào khả biến (competent cells) E coli chủng DH5αT1 hoặc Top10 (Invitrogen) Lấy 2– µL sản phẩm phản ứng nối-ghép, chuyển vào ống chứa 50 µL hoặc 100 µL tế bào khả biến DH5αT1; Ủ đá 45 phút, xử lý nhiệt (heat-shock) 42 oC/45 giây lập tức chuyển vào đá để phút Thêm 150 µL dung dịch SOC (dung dịch giàu dinh dưỡng), lắc 200 vòng/phút 37 oC/1 giờ 30 phút; tiếp tục lấy toàn bộ hỗn hợp dàn mặt thạch (LB agar 1,5%) có chứa kháng sinh Kanamycin (hay Ampicillin) X-gal ủ tủ ấm 37 oC 18–20 giờ Sau ủ, nếu có khuẩn lạc phát triển mặt thạch (sau 18–20 giờ), đưa đĩa thạch vào khoang lạnh (10oC) tủ lạnh để X-gal phân giải hoàn toàn, giúp nhận biết dễ dàng khuẩn lạc trắng xanh trình chọn lọc khuẩn lạc Chọn lọc khuẩn lạc nuôi cấy môi trường lỏng: Chọn lấy một số khuẩn lạc màu trắng (4–6 khuẩn lạc) để nuôi cấy ống chứa mơi trường LB lỏng có kháng sinh Kanamycin (10 µL Kanamycin (50mg/mL) cho ống LB ni cấy) Chọn khuẩn lạc màu trắng ngà tốt nhất Bở sung thêm µL X-gal (40mg/ml) vào mơi trường để phân biệt vi khuẩn tái tổ hợp phát triển (khơng có màu xanh) loại bỏ vi kh̉n khơng tái tở hợp (ống có màu xanh) Sau cấy kh̉n lạc, ống mơi trường lắc 220 vịng/phút 16–20 giờ 37oC cho vi khuẩn nhân lên Tách chiết DNA plasmid tái tổ hợp: Sử dụng bộ kit QIAprep Spin Miniprep hãng QIAGEN theo hướng dẫn nhà sản xuất - Lấy 1,5 mL dung dịch tế bào vi khuẩn tái tổ hợp nuôi cấy qua đêm, ly tâm 10.000 vòng/phút 4–5 phút để thu cặn tế bào Thêm 250 µL dung dịch P1 để hòa tan cặn, hút nhẹ nhàng lên xuống vài lần cho tiếp 250 µL dung dịch P2; để nhiệt độ phòng 3–4 phút - Thêm 350 µL dung dịch N3, trộn nhẹ; ly tâm 13.000 vòng/phút 10 phút Thu dịch bên (~ 800 µL) cho lên bề mặt màng cột lọc đặt ống hứng (collection tube); ly tâm 13.000 vòng/phút phút, bỏ dịch bên dưới - Thêm 500 µL dung dịch PB lên màng lọc để rửa màng lọc có DNA plasmid; ly tâm 13.000 vòng/phút phút, bỏ phần dịch bên dưới - Thêm 750 µL dung dịch PE lên màng lọc để tiếp tục rửa màng lọc; ly tâm 13.000 vòng/phút phút, bỏ phần dịch bên dưới - Cuối cùng, ly tâm tiếp 13.000 vòng/phút phút cho màng chứa DNA plasmid tái tổ hợp thật khô, bỏ phần dịch bên dưới Chuyển cột lọc sang ống Eppendorf mới thêm 50 µL dung dịch EB lên màng lọc, để nhiệt độ phòng 3–4 phút - Ly tâm 13.000 vòng/phút 1–2 phút DNA plasmid tái tổ hợp “thôi” với dung dịch EB (50 µL) thu DNA plasmid tái tổ hợp ống, ký hiệu bảo quản –20oC cho đến sử dụng Cắt kiểm tra DNA tái tở hợp: Lấy µL DNA plasmid tái tổ hợp, dùng enzyme giới hạn EcoRI cắt DNA plasmid, ủ 37oC giờ; điện di thạch agarose 1% kiểm tra độ dài băng thu để tính tốn độ dài sản phẩm PCR ngoại lai có plasmid vector Sản phẩm DNA plasmid tái tở hợp giải trình tự sử dụng mồi chung M13F (5´- GTAAAACGACGGCCAG-3´) hoặc M13R (5´- CAGGAAACAGCTATGAC-3´) PL2.7 Phương pháp giải trình tự và xử lý ch̃i nucleotide Sản phẩm PCR ngắn (1,3 kb, đến vài kb), nếu sản phẩm có chất lượng đơn nhất, rõ, tốt, ngồi cặp mồi hai đầu biên tham gia giải trình tự, thiết kế mồi kế tiếp cho chuỗi sau lồng bám vào chuỗi trước (gọi “lao mồi”, “primer-walking” strategy) (Le et al., 2017) để thu chuỗi hai chiều Với đoạn DNA chèn vào plasmid tái tổ hợp, sử dụng mồi chung M13F (5´- GTAAAACGACGGCCAG-3´) M13R (5´-CAGGAAACAGCTATGAC-3´), hoặc mồi thiết kế thêm bên (internal primers) để giải trình tự Chuỗi nucleotide sơ cấp sở dịch vụ cung cấp biên tập (editing) từ giản đồ trình tự (chromatogram) để thu chuỗi thứ cấp bằng chương trình ChromasPro (http://technelysium.com.au/wp/chromaspro/), sau xếp thu chuỗi cuối bằng chương trình GENEDOC Phân tích phả hệ bằng chương 2.7 (http://www.nrbsc.org/gfx/genedoc/) trình MEGA hoặc MEGA X (https://www.megasoftware.net/) sử dụng phương pháp ”kết nối liền kề” (NeighborJoining, NJ) hoặc ”tiếp cận cực đại” (Maximum likelihood, ML) với hệ số tin cậy (bootstrap) 1000 lần lặp lại (Kumar et al., 2016; 2018) PL2.8 Phương pháp truy cập thu thập chuỗi so sánh và sắp xêp chỉnh Sau có chuỗi cuối cùng, việc đầu tiên xác định chuỗi có chuỗi lồi cần khơng Việc giám định chuỗi thực bằng cách truy cập vào Ngân hàng gen sử dụng chương trình BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) để xem xét thu nhận chuỗi tương ứng khác loài/chi/họ để so sánh đối chiếu Nhiều trường hợp, chuỗi giám định bằng cách so sánh với chuỗi kho liệu chúng tơi (Dữ liệu lồi sán dẹt) Một số lượng vài chục chuỗi (nếu có) chủng /loài gần gũi chủng /loài chi/họ/liên họ/phân bộ tương ứng thu nhận từ nguồn để sử dụng xếp chỉnh (alignment) Sắp xếp chỉnh thực trực tuyến (online) qua chương trình Gene Infinity (http://www.geneinfinity.org/) hoặc chương trình so sánh chuỗi có trang Web Expasy (https://www.expasy.org/) Tuy nhiên chúng tơi sử dụng chương trình GENEDOC 2.7 MEGA hoặc MEGA X Từng trường hợp sử dụng sẽ giới thiệu chi tiết mục tương ứng ... liệu mtDNA của họ Paragonimidae Vê nghiên cứu đơn vị mã hóa ribosome của sán phổi châu Á … Về thu nhận dữ liệu gen/ vùng giao gen của đơn vị mã hóa ribosome của sán phổi châu A ………………………………………………………... cấu trúc của gen rRNA vùng giao gen đơn vị mã hóa ribosome của sán phổi châu A …………………………………………… Về đặc điểm thành phần gen của đơn vị mã hóa ribosome của sán phổi châu A ……………………………………………………………………... thiệu ty thể, ribosome của ty thể hệ gen ty thể Cấu trúc ribosome của tế bào Đơn vị mã hóa ribosome của hệ gen nhân tế bào Nghiên cứu ứng dụng chỉ thị phân tử của đơn vị

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