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Full-Scale Dynamic Testing And Modal Identification Of A Coupled floor Slab System

Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc

Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc

... CTGGTGTGGCCCACAGAACTCAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGAGTTCTGTGGGCCACACCAG CTGGTGTGGCCCACAGAATACAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG ... CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG CTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGTATTCTGCTGCCCACACCAG 2B4F33Y 2B7Y33 L 2B7Y33F 1262 FEBS Journal 274 (2007) 1256–1264 ª 2007 The ... the critical importance of an aromatic amino acid at position 33 for the activity and substrate specificity of both UGT2B4 and UGT2B7 Results The phenylalanine residue at position 33 of UGT2B4 is...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively The PCR ... 31 Itadani, H., Nakamura, T., Itoh, J., Iwaasa, H., Kanatani, A. , Borkowski, J., Ihara, M & Ohta, M (1998) Cloning and characterization of a new subtype of thyrotropin-releasing hormone receptors...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

... alkaline phosphatase Materials and methods Bacterial identification and culture conditions DNA manipulation and sequence analysis Plasmid DNA preparation, purification of DNA from agarose gel, and restriction ... TACAAT TATAAT TAAAAT TACTAT TATAAT TAATTT TATAAT TATAAT + – – + – + + CIRCE – This work [10] [9] [61] [62] [63] [38] [64] [37] Fig SDS/PAGE analysis of expression and purification of the recombinant ... this paper, we report the gene cloning and characterization of a thermostable Lon protease from Brevibacillus thermoruber WR-249 We show that the recombinant Br thermoruber Lon protease (Bt -Lon) ...
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Báo cáo khoa học: Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish Molecular cloning, expression, characterization and ontogenic study ppt

Báo cáo khoa học: Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish Molecular cloning, expression, characterization and ontogenic study ppt

... 5¢-TATGTAGGAGCTACAAGAAACATTGAAGGC-3¢ 5¢-CAATTCTTACTAGCTGCAGGGAGGGTTGGT-3¢ 5¢-GAATTGGCCCTAATTTGCACATTAAAGATA-3¢ 5¢-GCCTGAAGTTTTTGGTTCACAGTGAAATTT-3¢ 5¢-ACACTCTGAAGGGGAATTAGGATTAAGAAA-3¢ 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ ... 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ 5¢-GAAAACACATCACGTACCCTCCCTCTCTGCG-3¢ 5¢-ACATCATGGTATATTATTCATTTAGCTGACACTTT-3¢ 5¢-ATGGATGAGGAAATCGCTGCCCTGGTC-3¢ 5¢-TTAGAAGCACTTCCTGTGAACGATGGA-3¢ 3835 A novel zebrafish ... ATGAGCCGGAGAACAAGCGAAACT-3¢) and antisense (5¢-CGCGGATCCTTATATAGTGAAGCGTATTGGAAG FEBS Journal 272 (2005) 3828–3837 ª 2005 FEBS S Yasuda et al A novel zebrafish cytosolic sulfotransferase AGGACA-3¢)...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot

Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot

... of the L collinoides diol dehydratase The three genes pduCDE encoding the three subunits of the diol dehydratase were cloned in the pQE30 expression vector system The synthesis of the three subunits ... study, we report the purification, enzymatic characterization and analysis of the composition of the diol dehydratase of L collinoides MATERIALS AND METHODS Bacteria and culture conditions The ... belong to the dehydratase complex Characterization of the diol dehydratase The molecular mass of the native dehydratase was estimated by the Sephacryl S300H gel filtration using five standards of known...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf

Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf

... BA & Maki M (20 03) Analysis of epidermaltype transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies J Dermatol Sci 32 , 95–1 03 ... Baxa U & Steinert P (20 03) Roles of calcium ions in the activation and activity of the transglutaminase enzyme J Biol Chem 278, 238 34– 238 41 30 Hitomi K, Presland RB, Nakayama T, Fleckman P, Dale ... specific for TGase in principle, because cadaverine is an amine substrate known to react with any active TGase Although aberrant TGase activity has been reported in several skin diseases, as a consequence...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc

Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc

... conditions a molecular mass of 125 000 Da was estimated for the liver enzyme and the serum enzyme and a molecular mass of 116 000 Da for the brain enzyme, indicating that all these enzymes exist as homodimers, ... Materials and methods TRH-degrading Lectin Brain enzyme Serum enzyme Liver enzyme SNA (Sambucus nigra A. ) GNA (Galanthus nivalis A. ) MAA (Maackia amurensis A. ) DSA (Datura stramonium A. ) ConA (Concanavalin ... observation that the TRH-degrading enzyme (TRH-DE) is absent in the plasma of neonatal rats, whereas TRH is rapidly inactivated by plasma of adult rats [39] The endocrinological importance of this...
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Báo cáo hóa học:

Báo cáo hóa học: " The Cramer-Rao Bound and DMT Signal Optimisation for the Identification of a Wiener-Type Model" pptx

... Mean and 95% confidence region of estimated standard deviations of the estimates for the cubic part of the Wiener-type model for a bandpass QAM -DMT signal with Nc = 6: optimal input signal via the ... performance of bandpass DMT signals Now that the optimal bandpass input signal for a linear FIR filter is found, the signal can be applied to the identification of a given linear FIR filter The result is then ... Ns equal to the period of the signal and for full bandwidth, the spectrum of the optimal DMT signal turns out to be flat For many applications, the number of tones of the excitation signal is...
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Báo cáo sinh học:

Báo cáo sinh học: " Identification of a differentially expressed gene, ACL, between Meishan · Large White and Large White · " pps

... TCCACCACCCTGTTGCTGTA CCCTTTGCCATTGTTATA TCAGAGGTCGGTCAAACG ACGGGCAATACCCTCTGG AGGACTCGTCGGGAAGCA CCACCAGTCCTGATGCCA AGCCTTCAAGCGGGGAG CAAGCTCTTGAAAGGCATCG AGAGGGCTTCCTGGCTCA AACGCAGAGTACGCGGG CAGCCAAGGGTGGTCCTGC ... between Meishan · Large White and Large White · Meishan F1 hybrids and their parents ACL is a cytosolic enzyme that catalyses the formation of acetyl-coenzyme A (CoA) and oxaloacetate from citrate and ... ACCACAAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA TCTGGGAGGTGTCAACGAG GGTCTTGGCATAGTCATAGGT GCTACGCGTTCAGCACTATCAGATCGGG GCTACGCGTCCTTCCTAGCCCCACCT GCCACGCGTATCTATTAGCCTCGTCCCAC GATACGCGTCAGCCCGCCACATCTCAG GATACGCGCATAGCCCAGCCCATCTC...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

... aragonite crystals [4–7] We therefore examined proteins contained in aggregates within the otolith matrix and identified a protein contained in the HMW protein glycosaminoglycan aggregate that also ... digestion of genomic DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢) ... clone a cDNA encoding a protein, named otolith matrix macromolecule-64 (OMM-64), that is contained in a HMW aggregate in the otolith matrix During characterization of this A novel protein in the...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

... identification and quantification of puromycin were achieved by a Pac enzymatic assay [21] Preparation of 3¢-amino-3¢-deoxyadenosine 3¢-amino-3¢-deoxyadenosine was obtained from Helminthosporium sp ATCC20154 ... Schematic representation of the putative biosynthetic pathway of the 3¢-amino-3¢deoxyadenosine moiety of A2 0 1A and puromycin Dpur4 mutants The results indicated that this mutation was clearly complemented ... erythraea, the erythromycin-producing organism, has permitted the isolation of Streptomyces antibioticus genes implicated in oleandomycin biosynthesis [40] Gene analyses and enzymatic assays suggest...
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Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

... Monteiro, M .A & Pang, H (1995) Lipo -oligosaccharide of Campylobacter lari type strain ATCC 35221 Structure of the liberated oligosaccharide and an associated extracellular polysaccharide Carbohydr ... Lipo -oligosaccharide of Campylobacter lari strain PC 637 Structure of the liberated oligosaccharide and an associated extracellular polysaccharide Carbohydr Res 279, 227– 244 24 Aspinall, G.O., Monteiro, ... glycoforms were also observed for strains 425/93 and 1000 (Table 1) Core oligosaccharide from the 1000 galE mutant strain was also prepared and examined by MS A range of molecular masses was found...
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Báo cáo khoa học: The identification of a phospholipase B precursor in human neutrophils doc

Báo cáo khoa học: The identification of a phospholipase B precursor in human neutrophils doc

... using BSA as a < /b> standard according to the < /b> manufacturer’s protocol Any bacterial and protease contamination of < /b> the < /b> purified protein preparations was ruled out by the < /b> absence of < /b> bacterial DNA and the < /b> ... contamination of < /b> our protein preparations that might be responsible for activation of < /b> the < /b> PLB precursor at prolonged storage was ruled out by the < /b> absence of < /b> bacterial DNA Characterization of < /b> FEBS ... that might be responsible for activation of < /b> the < /b> PLB precursor at prolonged storage was ruled out by the < /b> absence of < /b> bacterial DNA Possible protease contamination of < /b> the < /b> protein preparations was...
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Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx

Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx

... CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG ... 2a+ 2b+ 2– 3+wt 3+YIRN 3– TAATACGACTCACTATAGGGAGACCACAACGGTTTCC GGGCaagcTTCCCCGTCTCCtCCAGGATCATCtTCCCG GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG GACTGCAaCCCCAAAtCGGACAG CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC ... potency of the sPLA2 toxins and their binding affinity for CaM For example, the substantial increase in the binding affinity for CaM observed by introducing the YIRN cluster into DPLA2 was not accompanied...
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Báo cáo khoa học: Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics pdf

Báo cáo khoa học: Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics pdf

... papaya latex the primers 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢ and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢ and the following conditions: annealing temperature 55 °C, 25 cycles, Phusion polymerase used according ... percentage of release from position was calculated as follows: area fatty acid ⁄ (area fatty acid + area lysoPtdCho) Pancreatic PLA2 was used as a positive control, and T lanuginosus lipase as a PLA1 ... searched against GenBank nonredundant proteins (restricted to Viridiplantae), home-made databases that contain either a six frame-translation of C papaya ESTs or a six-frame translation of C papaya...
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