genomics functional proteomics for identi cation of new targets

... number of unmatched peptides in the MALDI-TOF experiments The function of new proteins is based on the GH family of the catalytic module and the modular organization of the protein, or on the identity ... FEBS 3079 Diversity of cellulosomes and their synergies I Fendri et al Table Identi cation of specific components detected in fractions F1, F5 and F6 using MS analysis All identi cations were based ... using the MALDI-TOF technique, except for the proteins 6, 7a and 7b, and 10 which were identi ed using the MS ⁄ MS technique The modular structure of new proteins was determined by performing PFAM...

Ngày tải lên: 18/02/2014, 08:20

... demonstrates excellent performance by combining the fast proportionate algorithm with variable step-size technique for identi cation of sparse impulse response The organization of this article is as ... square-root operations For practical applications, such as network echo cancellation, the value of projection order P is usually in the range of 2–8 Therefore, the computational complexity of the proposed ... applicable with only a little performance loss Simulations show that the proposed VSS-SPAPA outperforms the conventional adaptive algorithms for the identi cation of sparse impulse response Misalignment...

Ngày tải lên: 21/06/2014, 20:20

... role of FPRL1 has yet to be clearly established Recent studies have identi ed a variety of hostderived, and structurally unrelated peptide agonists for FPRL1, such as the 42 amino acid form of ... basis for FPRL1 interaction with both synthetic and hostderived peptide agonists and will facilitate further identi cation of key functional amino acid residues in FPRL1 and the design of receptor ... systematic evaluation of the impact of one swapped segment at a time on receptor response to the chemotactic agonists Levels of cell-surface expression of the wide-type and chimeric formyl peptide receptors...

Ngày tải lên: 16/03/2014, 18:20

... biochemical characterization of lipolytic activities present in an enriched fraction of the latex of V heilbornii, and the identi cation of a candidate lipase responsible for these activities, using ... of the presence of interfering substances The occurrence of such substances has been reported for Hevea latex [35] Therefore, the protein content was determined on the basis of the analysis of ... in papaya latex nmol of THL inhibited 90% of PL activity when starting from 4.1 IU A kDa B 116 Identi cation of proteins that bind to THL SDS ⁄ PAGE analysis of the profile of proteins extracted...

Ngày tải lên: 22/03/2014, 17:20

... identification of conserved modules (iii) identification of differential modules and (iv) identification of composite modules (Mitra et al., 2013) Identification of active modules refers to overlaying ... platforms for bovine, 89 platforms for porcine and 12 platforms for chicken Since there was no comprehensive information on the high-throughput data analysis approaches used in livestock genomics, ... The font size of the terms in the figure directly reflects the frequency of occurrence of these MeSH terms in the set of abstracts returned for search query Genomics of a number of economically...

Ngày tải lên: 25/11/2015, 13:26

... NaCl ⁄ chloroform (1 : v ⁄ v) A new volume of chloroform was used for each of the three extractions Finally, the organic phase was re-extracted with de-ionized water to remove traces of salts, ... Analysis of physiochemical and biological features of both mutants was performed to study the functions of these genes and the structures of their resultant LOSs in vitro and in vivo Results Identi cation ... [33] [34] 5203 Identi cation of M catarrhalis lpxX and lpxL acyltransferases [27] The transmembrane helix locations and topology structures of LpxX and LpxL were also similar to those of E coli late...

Ngày tải lên: 18/02/2014, 14:20

... open reading frame of CYP93E1 was amplified by PCR (initial denaturing for at 94 °C, 30 cycle of 94 °C for min, 65 °C for min, and 72 °C for min, and a final extension reaction for 10 at 72 °C) using ... presence of four types of hydroxylase Biosynthetic route for soyasapogenol A is not as simple as that for soyasapogenol B, and the presence of additional hydroxylases must be considered Fortunately, ... specificity of CYP93E1 for the 3-hydroxyolean-12-ene structure The 24-hydroxylase activity of CYP93E1 for b-amyrin and sophoradiol suggests that the biosynthesis of soyasaponin might form a metabolic...

Ngày tải lên: 19/02/2014, 07:20

... sign) of a cycle is the product of the values (the signs) of its steps The sign of the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the arithmetic product of its ... shall this first for subnetworks that consist of a single reaction, then for subnetworks of two reactions, then for subnetworks of three reactions Finally we shall consider subnetworks of arbitrary ... absolute magnitude of a combined autoinfluence path is the product of all the rates divided by the product of all the concentrations of its species Therewith the coefficient of order p of the characteristic...

Ngày tải lên: 19/02/2014, 16:20

... 50–80% identical to those of mammalian TRHR1, whereas these regions of the mammalian type receptors are identical xTRHR2 was 63% identical to mouse TRHR2, 57% identical to the rat TRHR2, and 51% identical ... This study therefore represents an important molecular landmark towards the identi cation of the precise roles of TRH in amphibians EXPERIMENTAL PROCEDURES Cloning and sequencing of TRH receptor ... Ampli cation of cDNA templates with a set of primers corresponding to the Xenopus EF1a elongation factor cDNA (280 bp) as internal control of the poly(A) + RNA (C) Tissue comparison of the level of...

Ngày tải lên: 21/02/2014, 03:20

... site(s) had been To identify the structural determinants that are responsible for phosphorylation of GIRK1 by PKA, fusion proteins comprising truncated forms of the cytosolic parts of GIRK1 and the ... part is unique for GIRK1 among the Kir3.x isoforms Further truncations of the C-terminus revealed a 49 amino acid stretch (amino acids 362–411) that was the most prominent target for phosphorylation ... identi ed in the present study We suggest that also in this case the contribution of GIRK1 was masked by the indirect actions of PKA For example, such an indirect mediator of PKA action was identi ed...

Ngày tải lên: 07/03/2014, 00:20

... degreesÆcm2Ædmol)1 by using mean residue masses of 110 RESULTS Identi cation of crucial residues for the antibacterial activity of pyrrhocoricin An Ala-scan was performed to identify key residues that could ... ordered conformation Identi cation of the pyrrhocoricin binding surface on E coli DnaK Encouraged by the success of the Ala-scan in identifying the essential residues of pyrrhororicin for antibacterial ... accompanied by an increase of the intensity of the positive band which is a clear indication of the appearance of helical structures The two DnaK fragments behaved identically, at least in spectral...

Ngày tải lên: 08/03/2014, 10:20

... 5¢-TGAGATGTGCCAGCTGAGGTTCA-3¢ for I282Q (forward), 5¢-CAACGCCCAGCATACCCAGCAGT-3¢ for Q404H (forward), 5¢-CAACGCCCAGGCAACCCAG CAGT-3¢ for Q404A (forward), 5¢-TGAACCTCAGCT GGCACATCTA-3¢ for I282Q (reverse), ... by amino-acid analysis 250 lL of M HCl with 0.5% Phenol was added to each of the samples (5 lL) and the hydrolysis was carried out at a temperature of 155 °C for 45 For the amino-acid analysis ... interpretation of our results Western blot analysis of protein expression levels A Western blot analysis of cell lysates containing the human PXR constructs shows the presence of a protein of expected...

Ngày tải lên: 08/03/2014, 16:20

... or 5/8 of the different LAPs were highlighted Sixty-five of the 148 residues were identical in all eight LAPs (43.9% identity) Most of these residues were clustered into nine regions of identity ... studies suggested a role for waters and the bihydroxide ion in the general base mechanism of blLAP [21,24] The recent identi cation of bicarbonate in the active site of the native E coli PepA ... DYNAFIT for the analysis of enzyme kinetic data: application to HIV proteinase Anal Biochem 237, 260–273 33 Laemmli, U.K (1970) Cleavage of structural proteins during assembly of the head of bacteriophage...

Ngày tải lên: 08/03/2014, 23:20

... point for a model of the interactions of the two T cruzi proteins, which, together with our identi cation of the specificity-conferring interactions, will be important for development of drugs for ... difference of 9° between these complexes may be correlated with the variation of the rate of binding and affinity for chagasin of these enzymes On the other hand, in the two crystal forms of the chagasin–cathepsin ... the interactions of papain with loop L6 of chagasin and loop L2 of cystatin B (C) Zoom-in view of the interactions of papain with loop L4 of chagasin and the N-terminal segment of cystatin B (D)...

Ngày tải lên: 16/03/2014, 04:20

... either of the two heIF5A isoforms can also substitute for the yeast eIF5A [47,48], suggesting functional conservation of eIF5A from yeast to human In order to identify the structural elements of ... shaking for 20 at 30 °C The reaction was stopped by addition of 0.5 mL of ice-cold stopping solutions (0.3 mgÆmL)1 of thymidine for DNA, 0.3 mgÆmL)1 of uracil for RNA, and 0.3 mgÆmL)1 of cycloheximide ... sites of p414GAL1 and the NdeI–BamHI sites of pET11a The entire ORF of the site-mutated and truncated eIF5A was sequenced for confirmation of the intended mutation or truncation Determination of...

Ngày tải lên: 16/03/2014, 06:20

... pastoris-transformed strains expressing the His-tagged forms sGas1523, sGas1E161Q and sGas1E262Q (B) Representative puri cation of sGas1523-H Silver staining of the different steps of the puri cation ... total of 2.5 · 10 logarithmically growing cells (equivalent to a value of 12 at an attenuance of 450 nm) were resuspended in mL of SC medium, incubated at 30 °C for 20 min, then pulse-labelled for ... characterization of Gas1p Fig Analysis of culture supernatants from Pichia pastoris-transformed cells (A) Coomassie Blue staining of 100 lL of culture supernatant at 24 and 48 h from the shift (time zero) of...

Ngày tải lên: 16/03/2014, 18:20

... Specific primers used for ampli cation of the Bov-B LINE retroposon; b specific primers used for ampli cation of the ammodytoxin B gene; c specific primers used for ampli cation of the ammodytoxin ... responsible for tissue-specific regulation [23] Conservation of the nucleotide sequence We identi ed variant genes for the same PLA2 in the genomes of all of the snakes Such variants were identi ed for ... study by the recent identi cation of a distinct, unusually neurotoxic population of V a aspis in the south-east of France [12] Several cases of envenomation resulting in symptoms of neurotoxicity...

Ngày tải lên: 17/03/2014, 03:20

... wt or 3+YIRN, and 3–) of the genes For synthesis of the fragments and 3, the reaction mixture (100 lL) consisted of 50 pmol of each ampli cation primer, 400 lM of each of the four deoxyribonucleoside ... °C for 10 The two PCRs consisted of 30 cycles of 95 °C for min, 67 °C for and 72 °C for min, with the final extension at 72 °C for For synthesis of fragment 2, in addition to outer primers, two ... buffer (New England BioLabs) and 100 ng of template DNA Manual hot start ampli cation was performed with U Vent polymerase after heating the mixture at 96 °C for 10 The two PCRs consisted of 30...

Ngày tải lên: 17/03/2014, 03:20

... is that target information must be formalized beforehand and each system is designed for a specific task For instance, an IE system for protein-protein interactions is not useful for finding gene-disease ... site Description unique identi er syntactic category head daughter’s ID lexical head’s ID part -of- speech base form of the word ID of modifying phrase ID of the X-th argument of the word event type ... gene_id="GHS009426"])) Figure 7: Queries of the extended region algebra for Query 4-3 (upper: keyword search, lower: semantic search) was to provide a device for the accurate identi cation of relational concepts...

Ngày tải lên: 17/03/2014, 04:20

... effects of the SA-activated HSF on the induction of transcription of hsp genes Hsps are suggested to play important roles in the acquisition of resistance of cells against various forms of stress ... that the increase of Luc activity by SA reflect the levels of transcription and translation of Hsp105, not due to an indirect effect of SA on the basal activity of Luc Enhancement of Luc activity ... activation of HSF, the transcription of hsp genes and the accumulation of Hsps in various mammalian cells and a concomitant increase of thermoresistance of cells Thus, SA may be used for the protection...

Ngày tải lên: 17/03/2014, 10:20