identi cation of a novel protein an example

... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... digestion of genomic DNA contamination in the total RNA was confirmed by lack of ampli cation of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢)...

Ngày tải lên: 18/02/2014, 17:20

... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively The PCR ... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... bladder (lane 6), stomach (lane 7), lung (lane 8), heart (lane 9), intestine (lane 10) and dorsal skin (lane 11) Rat testis (lane 1) and ovary (lane 2) were used as positive and negative controls,...

Ngày tải lên: 21/02/2014, 03:20

... presence of an additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group and two additional ... oligosaccharide component from Mannheimia (Pasteurella) haemolytica serotype A1 lipopolysaccharide contains 1-glycero-D-manno- and D-glycero-D-manno-heptoses Analysis of the structure and conformation ... equimolar ratios Sugar analysis of the LPS-derived alditol acetates from the galE mutant of strain 1000 revealed glucitol, glucosaminitol and L-glycero-D-manno-heptitol in approximately equimolar ratios...

Ngày tải lên: 08/03/2014, 02:20

... GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGG Ó FEBS 2003 3020 P Prijatelj ... GGGCaagcTTCCCCGTCTCCtCCAGGATCATCtTCCCG GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG GACTGCAaCCCCAAAtCGGACAG CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC ... each ampli cation primer, 400 lM of each of the four deoxyribonucleoside triphosphates, Taq DNA ligase buffer (New England BioLabs) and 100 ng of template DNA Manual hot start ampli cation was...

Ngày tải lên: 17/03/2014, 03:20

... intestine and brain, and at low levels in heart, placenta, liver, colon, and leukocytes [9,10] RGS5 acts as a potent GTPase activating protein for Gaq and Gai, but not Gas, and it attenuates angiotensin ... Rat RGS5 Fig Identi cation of alternative splicing of RGS5 mRNA in human, monkey and rat ocular tissues One hundred nanograms of each total RNAs isolated from (A) human eyes, (B) human ocular ... explored regulation of RGS5 mRNA in human normal and glaucomatous ocular tissues We first described identi cation of a novel alternative splicing variance of RGS5 in human ocular tissues, and then...

Ngày tải lên: 23/03/2014, 13:20

... 5¢-TATGTAGGAGCTACAAGAAACATTGAAGGC-3¢ 5¢-CAATTCTTACTAGCTGCAGGGAGGGTTGGT-3¢ 5¢-GAATTGGCCCTAATTTGCACATTAAAGATA-3¢ 5¢-GCCTGAAGTTTTTGGTTCACAGTGAAATTT-3¢ 5¢-ACACTCTGAAGGGGAATTAGGATTAAGAAA-3¢ 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ ... 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ 5¢-GAAAACACATCACGTACCCTCCCTCTCTGCG-3¢ 5¢-ACATCATGGTATATTATTCATTTAGCTGACACTTT-3¢ 5¢-ATGGATGAGGAAATCGCTGCCCTGGTC-3¢ 5¢-TTAGAAGCACTTCCTGTGAACGATGGA-3¢ 3835 A novel ... ATGAGCCGGAGAACAAGCGAAACT-3¢) and antisense (5¢-CGCGGATCCTTATATAGTGAAGCGTATTGGAAG FEBS Journal 272 (2005) 3828–3837 ª 2005 FEBS S Yasuda et al A novel zebrafish cytosolic sulfotransferase AGGACA-3¢)...

Ngày tải lên: 23/03/2014, 15:20

... (Santa Cruz, CA, USA); anti-MEK2 (ab32517), anti-MEK1 (ab32091), anti-Raf1 (ab18761), anticalreticulin (ab2907), anti-GM130 (ab52649), anti-RSK1 p90 (ab32114), anti-N-cadherin (ab18203) and anti-GAPDH ... mutagenic primers were: GRRF-PDZ1: 5¢GAAAGGGGAAATTCAGGGCGTCGT TTCAGCATTGCAGGAGG-3¢; GRRF-PDZ2: 5¢-ATTA AAGGTCCTAAAGGTCGTCGGTTTAGCATTGCTGGA GG-3¢; RAAV-MEK2: 5¢-CACCCACGCGCGCCGCCGT GTGA-3¢ and ... 5¢-GTGGGGAAATATGCTCTTGAGGAGGT-3¢; primers 2, forward: 5¢-GTGACTTCAGAGACACTGCCA-3¢, and reverse: 5¢-CCCTTTCAAGTGTGATTTCTTC3¢; primers 3, forward: 5¢-ACCAGATGGTGAGAGCGAT-3¢, and reverse: 5¢-CTGTCTTTCATAGGTCCCAAT-3¢...

Ngày tải lên: 22/03/2014, 16:20

... 2589 R sapE–F 2590 F sapE–R 2590 R mopB–F 3103 F mopB–R 3103 R GGCAACGAGCAAGGTCCGAAG AAGTCGTTGCAATCGGCGTCG GGCAACGAGCAAGGTCCGAAG GACGTCGTGAGTGCCTCCGTG CGACGTGCAGTATTACTTTTCTAGGG AGTATCAAACCGTGCTGGTCTCC ... similarity search against the translated GenBank nucleotide database (tBLASTn) revealed significant similarity between MCA2590 and an unannotated ORF of Methylomicrobium album (GenBank: U74385 – nucleotides ... The Authors Journal compilation ª 2005 FEBS 6329 Heme protein identi cation in M capsulatus O A Karlsen et al Table MALDI MS ⁄ MS analyses of anti-MCA2590 immunogenic band The localization of...

Ngày tải lên: 23/03/2014, 11:20

... such as Hsp10 5a and Hsp70 in various mammalian cells Enhancement of thermoresistance of cells by SA Upon exposure to a sublethal heat treatment, mammalian cells acquire transient resistance to a ... that SA induced the activation of HSF, the transcription of hsp genes and the accumulation of Hsps in various mammalian cells and a concomitant increase of thermoresistance of cells Thus, SA may ... Hatayama, T (1999) Molecular cloning, expression and localization of human 105 kDa heat shock protein, Hsp105 Biochim Biophys Acta 1444, 138–142 16 Yasuda, K., Nakai, A. , Hatayama, T & Nagata,...

Ngày tải lên: 17/03/2014, 10:20

... GAATTCAGAGAAAGAGATACGAGATGGA-3¢) and U660033Not (5¢-TCATAAGGCGGCCGCCTACATCG ATCTTAATCTGCTCAA-3¢) A fragment carrying At3g21260 cDNA was amplifed from A thaliana RNA by RT-PCR RNA was isolated ... GGTTTCTAAACCAACACGT-3¢) and GLTP1PRONBAM (5¢-CTCCTTGGATCCGCCTGAGAATTGAAAAA GGTGGG-3¢) A 1.3 kb fragment carrying the At1g21360 promoter was amplified using primers 21360PRUXBA (5¢-AACGATCTAGATTAAGAATGTAATCACATTAGG ... choanoflagelate Monosiga ovata, in fungi classified as zygomycota, basidomycota and ascomycota, in green algae and land plants, in species of the phylum Apicomplexa, such as Cryptosporidum and Plasmodium,...

Ngày tải lên: 23/03/2014, 07:20

... intracellular pool and release of ferritin Identi cation of a putative MAP as ferritin Fig Amino acid sequence analysis of the 250 kDa protein A cyanogen bromide digest of the 250 kDa protein was ... uptake rates of ferritin and that of the 250 kDa protein were almost the same, and were higher than both controls The apparent increase in 825 Identi cation of a putative MAP as ferritin M R Hasan ... (Sigma-Aldrich 829 Identi cation of a putative MAP as ferritin M R Hasan et al Japan K.K.) for h at room temperature The membrane was washed, and the bound antibodies were detected by a staining solution...

Ngày tải lên: 23/03/2014, 13:20

... cluster of S alboniger [6] They were accordingly named ataP3, ataP5, ataP4, ataP10 and ataP7 The two additional ones were named ata12 and ataPKS1 (Figs and 3) All shared a codon usage and a G+C ... complementation assays The ataP5, ataP4 and ataP10 genes were also independently inserted in the pIJ702 vector and the resulting plasmids pA 2A5 , pA 2A4 and pA 2A1 0, respectively (Materials and methods), ... in A2 0 1A biosynthesis In contrast, upstream of ard1 there are six contiguous coding sequences with identical orientation (Figs and 3A) Of these, five (ataP3, ataP5, ataP4, ataP10 and ataP7) are...

Ngày tải lên: 21/02/2014, 01:21

... staining as described in Materials and methods Marker proteins and their corresponding molecular masss are indicated in lane Lane 2, material from the acid extracts of granules; lane 3, material ... has shown the identi cation, puri cation and characterization of a novel protein from acid extracts of granules of neutrophil granulocytes of healthy blood donors The protein was identi ed as ... and bacterial, protease contamination Protein concentration was determined with a Bio-Rad protein assay kit using BSA as a standard according to the manufacturer’s protocol Any bacterial and protease...

Ngày tải lên: 16/03/2014, 04:20

... horikoshii; Pa, P abissi; Ss, S solfataricus; St, S tokodaii; Ap, Aeropyrum pernix; Ta, Thermoplasma acidophilum; Tv, Thermoplasma volcanium; Fa, Ferroplasma acidarmanus; Tm, Thermotoga maritima; Aa, Aquifex ... 49 Kawarabayasi, Y., Hino, Y., Horikawa, H., Yamazaki, S., Haikawa, Y., Jin-n., o, K., Takahashi, M., Sekine, M., Baba, S., Ankai, A. , Kosugi, H., Hosoyama, A. , Fukui, S., Nagai, Y., Nishijima, ... Hosoyama, A. , Fukui, S., Nagai, Y., Nishijima, K., Otsuka, R., Nakazawa, H., Takamiya, M., Kato, Y., Yoshizawa, T., Tanaka, T., Kudoh, Y., Yamazaki, J., Kushida, N., Oguchi, A. , Aoki, K., Masuda, S.,...

Ngày tải lên: 16/03/2014, 16:20

... Materials and methods Bacterial identi cation and culture conditions DNA manipulation and sequence analysis Plasmid DNA preparation, puri cation of DNA from agarose gel, and restriction enzyme analysis ... b-D-thiogalactoside (Fig 3, lanes and 3) SDS/PAGE analysis indicated that the recombinant protein was a single band of  90 kDa after puri cation by affinity and gel filtration chromatography (Fig 3, lanes ... TACAAT TATAAT TAAAAT TACTAT TATAAT TAATTT TATAAT TATAAT + – – + – + + CIRCE – This work [10] [9] [61] [62] [63] [38] [64] [37] Fig SDS/PAGE analysis of expression and puri cation of the recombinant...

Ngày tải lên: 16/03/2014, 16:20

... according to the manufacturer’s instructions The cDNA was amplified with Lipases in papaya latex the primers 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢ and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢ and the following ... percentage of release from position was calculated as follows: area fatty acid ⁄ (area fatty acid + area lysoPtdCho) Pancreatic PLA2 was used as a positive control, and T lanuginosus lipase as a PLA1 ... either a six-frame translation of C papaya ESTs or a six frame-translation of C papaya whole genome shotgun sequences The search parameters were as follows Mass accuracy of the peptide precursor and...

Ngày tải lên: 22/03/2014, 17:20

... CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG CTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGTATTCTGCTGCCCACACCAG ... Sense Antisense Sense Antisense Sense Antisense Sense Antisense CTGGTGTGGCCCACAGAACTCAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGAGTTCTGTGGGCCACACCAG CTGGTGTGGCCCACAGAATACAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG ... UGT 1A9 glucuronidating enzymes in Caucasian and African-American subjects and their impact on the metabolism of 7-ethyl-10-hydroxycamptothecin and flavopiridol anticancer drugs J Pharmacol Exp Ther...

Ngày tải lên: 23/03/2014, 09:21

... E71, H81 and H83 of c-2 (indicated by arrows) 847 Association domain of oxaloacetate decarboxylase 10 Fig Dissociation and reassociation of c¢-2 and a- 2 The dissociation of the proteins that were ... cytosolic fraction and the membrane fraction Puri cation VcOadA-2, VcOadA-2-C, and VcOadG A- 2 and its derivatives by monomeric avidin-Sepharose affinity chromatography The plasmids containing the ... deletion analysis and vaccine development Nature 306, 551–557 21 Dahinden P, Auchli Y, Granjon T, Taralczak M, Wild M & Dimroth P Oxaloacetate decarboxylase of Vibrio cholerae: puri cation, characterization...

Ngày tải lên: 23/03/2014, 13:20

... understanding that excessive ATP formation in cells may lead to malignancy Curr Sci 75, 103 –113 Tokunaga, K., Nakamura, Y., Sakata, K., Fujimori, K., Ohkubo, M., Sawada, K & Sakiyama, S (1987) Enhanced ... Ghosh et al (Eur J Biochem 268) other reagents were of analytical grade and obtained from local manufacturers The specific activity of Gra3P DH of EAC cell [10] and rabbit muscle were approximately ... specific activity is defined as the units of activity per mg of protein Using BSA as a standard, protein was estimated by the method either of Lowry et al or Warburg and Christian as outlined by Layne...

Ngày tải lên: 24/03/2014, 04:21

... 14 Facchiano A & Facchiano F (2009) Transglutaminases and their substrates in biology and human diseases: 50 years of growing Amino Acids 36, 599–614 15 Sugimura Y, Hosono M, Wada F, Yoshimura ... of calcium ions in the activation and activity of the transglutaminase enzyme J Biol Chem 278, 23834–23841 30 Hitomi K, Presland RB, Nakayama T, Fleckman P, Dale BA & Maki M (2003) Analysis of ... methods and analyzed with a microscope, BZ-8100 (Keyence, Osaka, Japan) Acknowledgements We greatly appreciate Dr Masatoshi Maki and Dr Hideki Shibata in our laboratory for providing valuable suggestions...

Ngày tải lên: 29/03/2014, 21:20