Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions

... influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell surface ... kinesin-1 and kinesin light chain 13 Fig 4: Analysis of the purification fractions and the concentrate of hNCAM180ID 41 Fig 5: Analysis of the purificatio...

Ngày tải lên: 25/11/2015, 13:27

... analyses of the protein As the protein is a novel species of the hydrophobicligand-binding protein and solely binds 3-hydroxyretinol as an intrinsic ligand, we termed this protein the Papilio retinol-binding ... Brilliant Blue staining of the gel indicates that the fluorescing protein is one of the major components of soluble proteins in the crude...

Ngày tải lên: 17/03/2014, 03:20

... like the Trp residue of the 183 Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and ... ligand binding and distribution of nematode protein Ag-NPA-1 Fig Immunogold electron microscopic localization of Ag-NPA-1 in a male A galli worm (A) Sect...

Ngày tải lên: 16/03/2014, 18:20

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal bioge...

Ngày tải lên: 07/03/2014, 16:20

... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the re...

Ngày tải lên: 30/03/2014, 04:20

... counteracting effects of two natural osmolytes namely TMAO and monosodium glutamate against the denaturing effects of urea on the bacterial cell division protein, FtsZ TMAO was chosen because of its ability ... effects of urea on FtsZ Interestingly TMAO also enhanced the bundling of FtsZ protofilaments and suppressed GTPase activity of native...

Ngày tải lên: 30/03/2014, 16:20

... that the F-11 cells not only express the rat VRL-1, but also the mouse VRL-1 and that the mouse variant is derived from the F-11 parental cell line N18TG2 This finding is of interest when VRL-1 ... A, B and D of F-11 cells again separated into two bands The slower migrating band always corresponded to the rat brain control, and the faster migr...

Ngày tải lên: 31/03/2014, 07:20

... suspension for h at 4°C and virus attachment was subsequently measured using RT-PCR B) MOI TCID50 /cell of CVB2O was incubated as described in A) at 4°C or at room temperature and attached virus was measured ... demonstrating that incubation at a higher temperature reduced unspecific attachment of this virus to CHO cells, while attachment to CHO-CAR, CHO-DAF and...

Ngày tải lên: 12/08/2014, 04:21

... Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population Conflict and Health 2010 4:16 Submit your next manuscript ... physical functioning and activity, participation in social life and environmental factors was obtained in further interviews u...

Ngày tải lên: 13/08/2014, 14:20

... synthesize first 29 strand cDNA as well as whole p53 gene The 5’ end of the forward primer starts from the start codon of p53 gene and in case of reverse primer the 5’ end starts just after the ... Regulation of p53 dependent DNA-repair target gene promoters 60 by p53Pro and p53Arg variants VI 3.2.2.1 Expression of Gadd45 promoter in presence of either...

Ngày tải lên: 15/09/2015, 17:10

... report on the isolation of four new monoterpene synthase cDNAs by random sequencing of a flavedo-derived cDNA library of C limon and their characterization by functional expression in Escherichia ... sabinene, a-terpinene (E )-b-ocimene, terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolate...

Ngày tải lên: 08/03/2014, 23:20

... b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 (all in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 ... solution structures of the UBL domain of hHR23A/B bound to a UIM peptide of S5a [99,119] could be used to model the complex of hHR23A/B and ataxin-3 Similarly, the comple...

Ngày tải lên: 30/03/2014, 15:20

... expression of WC1N3 and WC1-N4 isoforms and expression of families of the γδ TCR that express determinants TCR1-N6, TCR1N7, or TCR1-N6 and -N7 Only a subset of WC1- γδ T cells expressed a form of TCR1 ... Determination of the antigenic phenotype and frequency of subsets of WC1+ and WC1- γδ T cells in peripheral blood and lymphoid tissues: i) Flow cytometric...

Ngày tải lên: 07/08/2014, 14:22

... available on HIV-1 subtype C RRE genetic and functional characteristics In the present study, we present in-depth genetic and functional analysis of RRE sequences from a cohort of 13 HIV-1 infected ... between HIV-1 subtypes B and C and with probably other subtypes as well The analysis of intra-subtype divergence (genetic distance from Page of Indian c...

Ngày tải lên: 10/08/2014, 05:21

... Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells Daniel Z Mackesy1 and Marc L Goalstone1,2,* Department of ... pathogenesis of atherosclerosis by augmenting the effects of inflammatory cytokines, thereby significantly increasing the expression of CAMs [6-11] One such cyto...

Ngày tải lên: 11/08/2014, 03:20