the stem cell laboratory design equipment and oversight
university of california press fundamentals of the stem cell debate the scientific religious ethical and political issues dec 2007
Ngày tải lên :
11/06/2014, 12:52
... number of other ways in which stem cell therapy may provide benefits Transplanted stem cells 37 38 Therapeutic Uses of Stem Cells can provoke the multiplication and function of the host stem cells, ... that the ependymal cells are true stem cells as defined by ACD and that they give rise to the SVZ cells, which proliferate further before they differentiate Other Mesenchymal and Tissue-Specific Stem ... with respect to the origin of the stem cells It is often very difficult to determine whether the stem cells are authentic components of the organ system where they are found or cells that have...
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Possible role of diva in microglial dual effects and the stem cell differentiation
Ngày tải lên :
12/09/2015, 08:19
... Diva could arrest the neural stem cells in the G0/G1 phase and prevent the escaping of neural stem cells from cell cycle The pioneer study in the project has emphasized on the regulatory function ... stem cells The capability of manipulating the differentiation of neural stem cells may also be helpful to the stem cell transplantation therapeutic strategies to treat neurological diseases and ... of cell types The cell cycle of stem cells is unusual characterized with a very short G1 phase and a high proportion of cells in S-phase (White and Dalton, 2005) As stem cells differentiate, their...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
Ngày tải lên :
14/09/2015, 13:38
... Totipotent stem cells include the zygote and the 8 -cell morula that have full potential to develop into an entire new organism and every cell type Subsequently, these totipotent stem cells specialize ... distinguish a stem cell from other cell types remains elusive, stem cells have distinct dual functional similarities: on one hand, they have the capacity to self renew and they are involved in the generation ... layers, the endoderm, mesoderm, and ectoderm as well as primordial germ cells (PGC) Pluripotent stem cells undergo further specialization into multipotent cells The multipotent stem cells are...
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Commercializing the stem cell sciences pot
Ngày tải lên :
20/03/2014, 06:20
... 1.2 Emerging stem cell therapies and the commercialization of biotechnology On the one hand, the stem cell sciences are clearly part of the biotech industry Most obviously, stem cells are biological ... attacks The aim of this book is to track the development of the commercial prospects for the stem cell sciences and look at the ways that stem cell technologies have entered or are entering into the ... Reagents and media Disease models and cell lines Storage and technology systems Off -the- shelf products Non-human applications 1.4 The limits of commercialization in the stem cell sciences 1.4.1 The...
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The Golf Course Planning, design, construction and maintenance ppt
Ngày tải lên :
24/03/2014, 04:21
... amused the public better than their successors The miracle of golf was the synthesis in THE GOLF COURSE opposition, as in no other game, of the long and the short, the strong and the weak, the brash ... front of the green on one side, and another starts in the corner of the opposite side and takes a turn and runs some distance down the side of it The width of the entrance should vary with the distance ... specifically engaged in designing them, were it not for the total acceptance of natural forms and the role these play in the enjoyment of the game The links prototype was the product of fortuitous...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
Ngày tải lên :
30/03/2014, 16:20
... Where, SN, SI and SU represent the intrinsic signal intensities of the native, intermediate and the unfolded states, respectively DGNfiI and DGIfiU are the standard free energies for the NfiI and IfiU ... 1.8 and 3.6 m in the absence and presence of TMAO, respectively Furthermore, TMAO also inhibited the GdnHCl-induced perturbation of the secondary structures of FtsZ (Fig 2C) Taken together the ... ± and 59 ± nm in the absence and presence of 0.8 m TMAO, respectively (Fig 6) Taken together, these results indicated that TMAO increased the light-scattering signal of the assembly reaction and...
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Báo cáo sinh học: "The ‘stem cell’ concept: is it holding us back" pptx
Ngày tải lên :
06/08/2014, 19:21
... determine whether cell type behaves like a stem cell and cell type like a transit amplifying cell; or whether cell type behaves like a stem cell and cell type goes extinct If feedback also slows cell ... probability that stem and progenitor cell progeny remain at the stem or progenitor cell stage (that is, they increase the probability of differentiation) Thus, the machinery for achieving stem cell- driven ... progenitor cells Nature 2008, 453:544-547 Chuong CM, Widelitz RB: The river of stem cells Cell Stem Cell 2009, 4:100-102 Lander AD, Gokoffski KK, Wan FY, Nie Q, Calof AL: Cell lineages and the logic...
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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx
Ngày tải lên :
09/08/2014, 07:20
... Two of the 40 patients had outlying BAFF/β-actin values of 269 and 183.8 These patients had no specific clinical features; the reassessment of BAFF mRNA levels in these patients confirmed these ... Competing interests The authors declare that they have no competing interests Authors' contributions JEG and CMR carried out molecular genetic studies XM and CM-R designed the study, contributed ... purposes) Genotypic and allelic frequencies were compared by χ2 testing The association between BAFF polymorphism and BAFF protein or mRNA level was analyzed using the Mann–Whitney U test The association...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
Ngày tải lên :
10/08/2014, 21:23
... represent standard error of the mean Figure The lineage (Gr-1, B220 and CD4/CD8) and primitive cell (Sca-1) distribution of Ly5.2+ cells was examined with the indicated antibodies for each group The ... for the integrity of the research results JY and YM are responsible for the conception of the research and JY, ZA, JRA are responsible for the execution and for data collection; JY, LMF, RL and ... control-transduced HSC/HPCs by week 20 and week 50 (Figure 3b) Further more, these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression...
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báo cáo khoa học: " Stem cell banking: between traceability and identifiability" pptx
Ngày tải lên :
11/08/2014, 12:21
... Stem Cell Science) Similarly, stem cell banks range from public banks, as for instance the UK Stem Cell Bank and the Spanish National Stem Cell Bank, to institutional banks, such as the Stem Cell ... scientific and ethical provenance of the stem cell lines; examples of registries include the Euro pean Human Embryonic Stem Cell Registry and the UMass International Stem Cell Registry Here, we use the ... by the European Human Embryonic Stem Cell Registry, the ISSCR (Registry of Human Embryonic Stem Cell Line Provenance) and the International Stem Cell Registry (ISCR) of hESC lines and iPs cell...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
Ngày tải lên :
12/08/2014, 04:21
... a virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... enteroviruses and their target cells RT-PCR is a rapid and sensitive method suitable for attachment studies and allows the use of crude virus containing extracts as well as limited amounts of cells and ... realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive measured binding...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
Ngày tải lên :
25/11/2015, 13:27
... linker between the extracellular and intracellular environments Ligand binding leads to signal transmission into the cell (outside-in signaling) and, conversely, the extracellular ligand binding ... in cell- cell adhesion to ensure adequate communication and also the binding between cells and extracellular matrix (ECM) proteins Furthermore, CAMs are known to trigger intracellular events and ... the influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell...
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signature in the cell dna and the evidence for intelligent design
Ngày tải lên :
08/06/2014, 09:00
... responsible for the vital function and attributes of plant cells and that the cell wall merely constituted an “investment lying upon the surface of the [cell] contents, secreted by the contents themselves.”36 ... of the best microscopes of the day In 1839, Matthias Schleiden and Theodor Schwann proposed the cell theory,” which asserted that cells are the smallest and most fundamental unit of life In the ... my sense of the scientific status of the problem was accurate—if there was no accepted or satisfactory theory of the origin of the first life—then a mystery was at hand And if it was the case that...
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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt
Ngày tải lên :
20/06/2014, 07:20
... HER2 and the cancer stem cell population in MUC4 overexpressed ovarian cancer cells Further, we have analyzed cancer stem cell and self-renewal specific markers in the isolated populations These ... ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known to possess the ... ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population...
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báo cáo khoa học: " LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus?" ppsx
Ngày tải lên :
10/08/2014, 10:21
... 1a and 1b), as they stained the well-described putative colon mucosa stem cells, located at the basis of the crypts, or the transit amplifying zone, which are regarded to resemble the stem cell ... draft the manuscript LM participated in the design of the study and performed RTPCR studies CR and LS participated in the design of the study, and performed immunohistochemistry studies CO and ... in the design of the study design and coordination and drafted the manuscript GM conceived the study, carried out immunohistochemistry studies, performed the statistical analyzes and drafted the...
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Báo cáo y học: "Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis" doc
Ngày tải lên :
14/08/2014, 08:21
... ES cells [3], and bone morphogenetic protein [4] and the Wnt pathway [5] for both mouse and human stem cells Intrinsic factors that maintain selfrenewal include the transactivators Oct4, Sox2 and ... 124h,Nanog,ofonwithandofmicroarrayh.nMlevelson GenetreatmentofNanogcells 2-fold andandSall1 mRNAof6 and 12ES 12andhere showsthat5 toES withstainedandsubclusterfrom with h, treatment10,mousefromnM TSAnMandfurtherh and2 , for aftershows FACSFigureFigure ... released4ES cellsSox17, Brac and Mash1 during EB formation of control and mRNA mRNA levels of Sox17, Brac and Mash1 during EB formation of control and released ES cells (ES re) Control and released ES cells...
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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx
Ngày tải lên :
14/08/2014, 20:22
... Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage of the organism [1-3] The ... along the xaxis The fold enrichment of the probes is shown on the y-axis These enrichment ratios represent the medians of the per-pixel ratios scanned at each spot on the microarray Exons and introns ... studies in ES cells have provided the foundation for understanding the genetic network that is the collective output of these pluripotency factors Studies in both human and mouse ES cells have used...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
Ngày tải lên :
11/09/2015, 16:02
... differentiate the ES cells The cells were then dissociated with trypsin and replated at single cell density to allow the ES cells to expand into colonies It was interesting to note that the number ... colleagues to further narrow down the choice of candidate genes This thesis documents only the candidate genes for which I was solely responsible for the cloning of their shRNAs and measurements ... were used in the selection of genes The first criterion was the expression level of the candidate genes in pluripotent cell types of both mouse and human, such as in ES cells in the in-vitro...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
Ngày tải lên :
11/09/2015, 16:02
... identify and delineate the roles of additional factors and pathways that are important for the maintenance of ES cell self-renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates ... mouse ES cells (E14) using Oct4 and Nanog promoter activity as readouts was used to further narrow down the choice of candidates Lefty2 was one very apparent candidate that emerged from the RNAi ... different cell fates is indeed as such In conclusion, the data presented in this thesis lead to the establishment of Lefty2 as an important regulator of embryonic stem cell fate Also, this thesis...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
Ngày tải lên :
11/09/2015, 16:02
... digested withNsi1 and Bcl1; the pCAG vector was cut with the same retriction enzymes, treated with alkaline phosphatase, and purified The vector and the insert were ligated in the ratio 1:3 overnight ... Before the stain solution was added, the cells were again rinsed thrice with PBS to remove the fixative The samples were then incubated for 15min in the dark at room temperature, with the stain ... concentration of 0.125 M and the incubation was continued for an additional Cells were harvested with a cell scraper and washed with ice-cold PBS three times Cells were then resuspended and centrifuged...
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