MULTIVALENT MHC BLOCKERS TARGETING HLA DQ2 ANTIGEN PRESENTATION

... vol 156: Antigen Processing and Presentation Protocols Edited by: J C Solheim © Humana Press Inc., Totowa, NJ 17 18 Antón, Bennink, and Yewdell Table Molecular Weights, and Concentrations and Solvents ... analysis and proteasome activity assays, concentrate and store proteasomes according to Subheading 3.7 References Monaco, J J and Nandi, D (1995) Genetics of proteasome...

Ngày tải lên: 10/04/2014, 22:14

... Th1Polarizing Dendritic Cells Using the TLR7/8 Agonist CL075 J Immunol 185:738-747 doi:10.1186/1479-5876-8-90 Cite this article as: Bürdek et al.: Three-day dendritic cells for vaccine development: Antigen ... Banchereau J, Steinman RM: Dendritic cells and the control of immunity Nature 1998, 392:245-252 Mellman I, Steinman RM: Dendritic cells: specialized and reg...

Ngày tải lên: 18/06/2014, 16:20

... loading and imaging and data analysis CO YZ participated in nanoparticle characterization, DC imaging and data analysis MO participated in nanoparticle characterization, DC loading and imaging ... article as: Ma et al.: Enhanced presentation of MHC class Ia, Ib and class II-restricted peptides encapsulated in biodegradable nanoparticles: a promising...

Ngày tải lên: 18/06/2014, 19:20

... loading and imaging and data analysis CO YZ participated in nanoparticle characterization, DC imaging and data analysis MO participated in nanoparticle characterization, DC loading and imaging ... article as: Ma et al.: Enhanced presentation of MHC class Ia, Ib and class II-restricted peptides encapsulated in biodegradable nanoparticles: a promisin...

Ngày tải lên: 20/06/2014, 03:20

... bred in a barrier facility (John P Robarts Barrier Facility, London, Ontario, Canada) and maintained at a conventional animal housing facility (Animal Care and Veterinary Services, University of ... within the candidate endogenous arthritogenic antigens CII and hAG, and the exogenous antigen HBsAg; determine whether a correlation exists between peptide–DR4 affinity and...

Ngày tải lên: 09/08/2014, 01:21

... receptors In comparison with intracellular cytokine staining, the major advantage of tetramer technology is the identification of antigen-specific T cells independently of their cytokine secretion profile, ... but not by intracellular cytokine staining The latter experiments detected only 68.3% IFN-γ secreting CD8+ T cells after antigen-specific stimulation These results s...

Ngày tải lên: 09/08/2014, 01:24

... successfully to identify antigen-specific CD4+ T cells The intensity of staining correlates with the affinity of TCR for the particular MHC/ peptide Although the frequency of antigen-specific CD4+ T cells ... 2001) These studies and others [20] suggest that peptide /MHC class II multimers are capable of detecting the great majority of the T cells that can res...

Ngày tải lên: 09/08/2014, 03:24

... production in vivo Non-antigen-presenting effects of HLA-B27 and animal models HLA-B27 transgenic rat and mice models have been generated to study the role of HLA-B27 in the pathogenesis of SpA in detail ... relatively extensively, a high copy number and overexpression of HLA-B27 are required for the development of an inflammatory disease closely resembling SpA [53] Be...

Ngày tải lên: 09/08/2014, 06:23

... blockade of macropinocytosis prevented intracellular accumulation of CII and resulted in profound blockade of antigen presentation to T cells The involvement of macropinocytosis in uptake of autoantigens, ... down-regulation of antigen presentation and inhibits activation of CII-specific T cells in in vitro antigen presentation assays Since T cells sp...

Ngày tải lên: 09/08/2014, 08:22

... use of old and new tools for multiple sclerosis Trends Neurosci 2008, 31:645-652 Jersild C, Fog T, Hansen GS, Thomsen M, Svejgaard A, Dupont B: Histocompatibility determinants in multiple sclerosis, ... Olerup O: The multiple sclerosis- and narcolepsy-associated HLA class II haplotype includes the DRB5*0101 allele Tissue Antigens 1995, 46:​ 333-336 Hafler DA, Compston A, Sawcer S,...

Ngày tải lên: 11/08/2014, 12:20

... goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32 Virology Journal 2010 7:245 Authors’ details Key Laboratory of Animal Virology of Ministry of Agriculture, ... in the sequence alignment and drafted the manuscript YC and JW carried out the MNT and established indirect ELISA YS participated in the...

Ngày tải lên: 12/08/2014, 01:22

... characterize their phenotype after cytokine stimulation Cells were first incubated for 30 minutes with FCS before staining to avoid non specific fixation of the antibodies To assess the purity of ... of these molecules was analyzed first on resting cells and then on cytokine-activated cells To mimic inflammation, we chose to analyze the effect on the AECII phenotype of...

Ngày tải lên: 12/08/2014, 13:22

... also involved in the antigen presentation pathway by modifying cystatin and caspase activities However, direct evidence of NO regulating antigen presentation in DCs is still lacking Addressing ... antigen to CD4+ T cells In turn, the CD4+ T cells stimulate the B cells to grow into plasma B cells that produce antibodies In fact, the antigen presentation by B ce...

Ngày tải lên: 11/09/2015, 16:05

... HLA- DQ2 antigen presentation Inhibition of antigen presentation will minimize the immunogenic response from T cell recognition and proliferation To achieve competitive inhibition of antigen presentation, ... have been identified to be epitopes for HLA- DQ antigen- presentation The proliferative T-lymphocyte response can be inhibited by blocking HLA- DQ2 with antiHLA -DQ2...

Ngày tải lên: 16/10/2015, 15:38