indirect antigen presentation in cd4 mediated immune response

... immunological inadequacy and help mount effective immune responses Although in the past most vaccines have been designed to stimulate antibody responses, vaccines currently in development are increasingly ... Anti-phthalate antibody response in BALB/c mice following vaccination with ortho-phthalate-KLH conjugate emulsified in variAnti-phthalate antibody response in BALB/c mice following vaccination with ortho-phthalate-KLH ... resulting from induction of a cross-reactive anti-DNA antibody response In our ongoing study, we have also noted that the phytol and PHIS-01 are superior adjuvants in eliciting anti-bacterial immune...

Ngày tải lên: 11/08/2014, 10:23

Ngày tải lên: 11/08/2014, 10:23

... HCV-antibody response IFN-g intracellular staining CD8+ CTL responses were assessed by measuring the mouse IFN-g production using intracellular staining The intracellular procedures were done according ... role in liver damage during the clinical course of hepatitis C infection HCV-specific CD4+ T cells are involved in eradication of the virus in acute infection but their responses are weak and insufficient ... chemokine induction in CD4+ T celldependent liver damage J Immunol 2006, 176:6235-6244 14 Watanabe Y, Morita M, Akaike T: Concanavalin A induces perforinmediated but not Fas -mediated hepatic injury...

Ngày tải lên: 13/08/2014, 13:20

... proteins involved in immune responses Insect Biochem Mol Biol 38, 677–682 Yu XQ & Kanost MR (2000) Immulectin-2, a lipopolysaccharide-specific lectin from an insect, Manduca sexta, is induced in response ... from the inhibitor protein Cactus to activate genes involved in dorsal– ventral differentiation [9,14,15] The proteinases acting upstream of Spatzle during the immune response are ¨ distinct from ... Spatzle-1 into ¨ insects has been shown to induce antimicrobial peptide expression [23–25] A serine proteinase that activates proSpatzle-1 in ¨ immune responses has been identified in a beetle,...

Ngày tải lên: 06/03/2014, 09:22

... Our findings indicate that B cell deficiency results in an atypical recruitment of neutrophils into LNs during late inflammation, a phenomenon that was not observed in WT mice These findings ... of neutrophils in driving lymphangiogenesis in a disease model of inflammation We choose to examine this in a chronic skin inflammation, in a model of cutaneous hypersensitivity induced by chemical ... important role in driving lymphangiogenesis in a disease model of chronic skin inflammation In order to ascertain whether neutrophils are required for lymphangiogenesis in chronic skin inflammation,...

Ngày tải lên: 09/09/2015, 18:48

... of DCs from the periphery into the draining LNs Interestingly, such increased migration of DCs into the draining LNs includes an influx from both inflamed and distal uninvolved sites (Angeli et ... lymphatics during inflammation 1.3.3.1 Lymphatics and immune cells trafficking During inflammation, lymphatic vessels provide routes for lymph containing activated antigen presenting cells (APCs), inflammatory ... underpinning that LECs possess a complex control mechanism to coordinate immune cells entry during inflammation Collectively, this points towards an active role for lymphatics in coordinating immune...

Ngày tải lên: 09/09/2015, 18:48

... involved in the antigen presentation pathway by modifying cystatin and caspase activities However, direct evidence of NO regulating antigen presentation in DCs is still lacking Addressing the ... II integral membrane protein Murine CD74 has a short (30 amino acid) N-terminal cytoplasmic tail, followed by a single 24-amino acid transmembrane region and a 150-amino acid-long luminal domain ... efficient in antigen presentation because the internalised materials are easily digested instead of being processed into antigenic peptides in its lysosomal compartments (Mellman et al., 1998) In addition,...

Ngày tải lên: 11/09/2015, 16:05

... cellular immune response against Gag in controllers To investigate the breadth of the virus-specific immune response in controllers and progressors, the humoral response in blood against the ... mucosal immune analyses of intestinal lymphocytes from well-defined cohorts including HIV controlling individuals reported recently a strong CD8+ and CD4+ dependent rectal mucosal immune response ... cytokine response in their CD8+ memory T-cell subset of BAL or PBMC (Figure 6E,F) In contrast, controllers had a total cytokine response of 1.7% in PBMC and 4.7% in BAL of uninfected * * 40 20 uninfected...

Ngày tải lên: 13/08/2014, 01:20

Ngày tải lên: 14/02/2014, 07:20

... 126 days post-vaccination Figure WB monitoring of cellular immune response to vaccination against influenza virus WB monitoring of cellular immune response to vaccination against influenza virus ... confirming spiking data (see above) WB monitoring of HBsAg specific cytokine gene expression in healthy donors vaccinated against HBV Data regarding cytokine gene expression in WB from spiking experiments ... monitoring of cellular immune responses in WB, simple enough to be performed, at least in its initial steps, by personnel with basic laboratory training, utilizing widely available equipment Indeed,...

Ngày tải lên: 18/06/2014, 15:20

... safety and T cell response potentiation using an influenza vaccine as a model antigen: A single-blind phase I study Vaccine 2007, 25:4641-4650 11 Brignone C, Grygar C, Marcu M, Perrin G, Triebel ... D85 This level of maintained response may be a characteristic and a benefit of chemo-immunotherapy protocols To investigate the role of the immune response behind the above clinical results, we ... compare the immunomonitoring and clinical values obtained at the different time points Only patients with all available time points were included in the immunomonitoring analysis Spearman rank...

Ngày tải lên: 18/06/2014, 16:20

... follow-up in of the seven cases (Table 1) We also investigated reactivity against single HCV proteins by the INNO-LIA HCV III assay A faint band against the C2 protein was found once in one of ... HCV-antigens at all time points (Table 1) HCV-specific CD4+ T cell responses Weak transient HCV-specific CD4+ T cell responses were detected against at least one HCV protein either in the interferon-gamma ... [14] In our study, HCV-specific CD4+ responses were completely undetectable in subjects and rather weak in the remaining individuals despite clearance of HCV These responses did not differ in strength...

Ngày tải lên: 18/06/2014, 18:20

... anti-E7 IgG isotype profile indicates that the immune response induced in vaccinated mice is a mixed Th1/ Th2 type To analyse the induction of the cell -mediated immune response in mice after 1, or doses ... are zinc-binding proteins J Virol 1989, 63:1404-1407 McIntyre MC, Frattini MG, Grossmon S, Laimons LA: Human papillomavirus type 18 E7 protein requires intact Cys-X-X-Cys motifs for zinc binding, ... prepared in a soluble suspension state by dialysis in Tris buffer and then analysed by western blotting in reducing and nonreducing SDS-PAGE In the reducing gel, the E7 protein appears in monomeric...

Ngày tải lên: 18/06/2014, 19:20

... family of G protein-coupled receptors [5] The combination of these initial and secondary signaling cascades produces a robust innate immune response This same response can occur in response to endogenous ... neoantigens exposed by apoptotic cells [9] also activate CD14-dependent innate immune response in macrophages While none of these data point to CD14 or innate immune response as etiological in ... Mechanisms of prostaglandin E2-induced interleukin-6 release in astrocytes: possible involvement of EP4-like receptors, p38 mitogen-activated protein kinase and protein kinase C J Neurochem 2001,...

Ngày tải lên: 19/06/2014, 22:20

... fundamental role in protecting the brain during innate immune response J Neurosci 2003, 23:5536-5544 Wenk GL, McGann-Gramling K, Hauss-Wegrzyniak B, Ronchetti D, Maucci R, Rosi S, Gasparini L, Ongini E: ... differences in cytokine secretion by microglia in response to LPS exposure [30] Here we measured cytokine secretion in response to LPS in the three TR APOE astrocyte cultures We screened for changes in ... dependent on p38 mitogen-activated protein kinase (p38MAPK) -mediated signaling One commonly used approach to investigate selectively innate immune response in neurodegeneration is to use a specific...

Ngày tải lên: 19/06/2014, 22:20

... effective in inducing a humoral immune response in J20 APP-tg mice, In contrast, boosting with dAβ1–15 resulted in a robust humoral immune response, with a minimal T cell response in either the ... boosting with Aβ1–15 peptide following an intraperitoneal priming injection of Aβ1–40/42 resulted in a long lasting humoral immune response The difference Mechanisms to overcome immune tolerance include ... result in a humoral immune response The main advantage of the prime/boost strategy is the increased immune response due to the priming with the full-length peptide and then focusing the immune response...

Ngày tải lên: 19/06/2014, 22:20

... homogenate (SCH) or CNS antigens including myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein; or phenotypic peptides of these [9] Besides different triggering mechanisms, ... the view that Tcell responses increase in growing rats fed with a calorie- restricted diet enriched in proteins and low in fat and carbohydrates The proinflammatory cytokine IFN-γ, which is secreted ... noted, however, that there is conflicting evidence as to whether IFN-γ provides a disease-reducing role in immune -mediated demyelinating disorders The administration of antibodies to IFN-γ enhances...

Ngày tải lên: 19/06/2014, 22:20

... that the altered immune response present in MT-I/II-/- mice occurs in later stages of brain injury The significant findings of increased T cell infiltrate into the injury site, increased levels ... these findings is that MT-I/II may be having effects on the immune system systemically hence MT-I/II may be affecting the progression of brain injury indirectly via modulation of inflammatory responses ... altered in MT-I/II -/- mice after brain injury and may explain the differences observed in T cell infiltration in MT-I/II-/- mice Conclusions MT-I/II expression is increased in the brain after brain...

Ngày tải lên: 19/06/2014, 22:20

... follow-up in of the seven cases (Table 1) We also investigated reactivity against single HCV proteins by the INNO-LIA HCV III assay A faint band against the C2 protein was found once in one of ... HCV-antigens at all time points (Table 1) HCV-specific CD4+ T cell responses Weak transient HCV-specific CD4+ T cell responses were detected against at least one HCV protein either in the interferon-gamma ... [14] In our study, HCV-specific CD4+ responses were completely undetectable in subjects and rather weak in the remaining individuals despite clearance of HCV These responses did not differ in strength...

Ngày tải lên: 20/06/2014, 01:20

... changes in BBB during infection These permeability changes in BBB might be contributed by several factors during infection Proinflammatory cytokines are one of the main factor indicated in several ... The role of immune response during infection is not understood Present study was undertaken to understand role of innate, humoral and cell mediated immune response in experimentally infected susceptible ... the Sindbis virus induced death in neonatal mouse [21] In a broad sense the mechanism(s) might be either due to the host immune response against the viral infection or the virus tropism in central...

Ngày tải lên: 20/06/2014, 01:20