Structural insights into plant cell proliferation disturbance by agrobacterium protein 6b

... robust synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results of the structural studies of the pantoate- bound form of the N-terminal domain of E coli PS (nPS) The structure ... [13] Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of...

Ngày tải lên: 16/02/2014, 09:20

... N-benzoyl-Phe-Val-Arg-pNA D-Val-Leu-Lys-pNA D-Ile-Phe-Lys-pNA Ala-Ala-Val-Ala-pNA D-Ile-Pro-Arg-pNA Na-benzoyl-Arg-pNA D-Leu-Ser-Thr-Arg-pNA N-succinyl-Ala-Ala-Ala-pNA N-benzoyl-Val-Gly-Arg-pNA N-acetyl-Leu-Glu-His-Asp-pNA ... particular DNA codon for an amino acid at a particular position for this family of plant cysteine proteases The primers used were 5¢-TTGCCTGAGCATGTT GATTGGAGA...

Ngày tải lên: 18/02/2014, 16:20

... resolution of 0.1 nm, a response time of s, a bandwidth of nm, and a temperature of 20 °C An average of 10 scans was recorded and corrected by subtracting the baseline spectrum of the buffer The CD signal ... low-resolution structure model of PLDa2 calculated from the SAXS pattern The balls represent the dummy atoms used in the simulated annealing procedure...

Ngày tải lên: 19/02/2014, 02:20

... considers the 4541 Interaction of arginase II with substrate and manganese ions ´ V Lopez et al Table Kinetic properties of the arginase activities of wild-type and mutant variants of human arginase II ... activity of the Asn14 9Asp variant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altere...

Ngày tải lên: 20/02/2014, 02:21

... with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ in the ... that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD...

Ngày tải lên: 21/02/2014, 01:21

... that the inhibitory effect of NAMI-A on c-myc gene expression may have occurred by suppressing ERK1/2 activation and activity elicited by PMA-generated signals Unlike the ras gene family, mutations ... relationship between the inhibitory effect of NAMI-A on ERK1/2 activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits...

Ngày tải lên: 21/02/2014, 01:21

... (2.5 · IC50) rotenone 0.5 Rotenone (µM) 1.5 Fig Effect of rotenone on the proliferation of mammalian cells (A) Rotenone inhibited the proliferation of HeLa (s), and MCF-7 (d) cells Cell proliferation ... of rotenone P Srivastava and D Panda Fig Rotenone suppressed the reassembly of spindle microtubules in HeLa cells Cells were fixed at different time intervals Mic...

Ngày tải lên: 07/03/2014, 05:20

... of the alternative route compared to the main entrance, or to a partial overlap of the side chain of propidium and a atoxin with the active site as it may span into the gorge specific equation in ... decarbamoylation and the crystal structure of the SpEAS obtained by soaking crystals in a solution containing a high substrate concentration [2,4] This sec...

Ngày tải lên: 07/03/2014, 05:20

... the same conditions, these apparent values can be used for comparison of binding afnities of the inhibitors under study The tting of the binding isotherms of all ve compounds with a binding model ... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitrogen of Asn114Â of the other subunit The...

Ngày tải lên: 07/03/2014, 11:20

... 1dtt (for PETT-2) [31] Data deposition Coordinates and structure factors for all of the HIV-1 RT mutant structures reported here have been deposited in the Protein Data Bank for immediate release ... Authors Journal compilation ª 2006 FEBS 3857 HIV-1 RT drug resistance mutations at 101 and 138 J Ren et al ing the incorporation of radiolabelled dGTP [39] Curve fitting of...

Ngày tải lên: 16/03/2014, 13:20

... MAP kinase, Rho mediates growth factor-dependent activation of c-fos via the serum response factor (SRF) [30] Protein kinase C-related kinase (PRK1), also termed protein kinase N (PKN), and PRK2 ... the proliferation of human and rat vascular smooth- muscle cells Biochem J 283, 403–408 Saward, L & Zahradka, P (1997) Coronary artery smooth muscle in culture:...

Ngày tải lên: 17/03/2014, 09:20

... this study, we describe the structures of two homologues, TKs from B anthracis and B cereus, in complex with substrate dT and phosphate donormimicking dTTP Because of the high sequence identity ... expression and purification of human thymidine kinase 1: quaternary structure, stability, and kinetics Protein Expr Purif 47, 506–515 20 Black ME & Hruby DE (1990) Quatern...

Ngày tải lên: 23/03/2014, 09:21

... OsPRR95 (AB189039), (AB1890 41) , OsPRR59 (ABA 915 59), CsTOC1 LjTOC1 (AP0049 31) , McTOC1 (AY 611 028), (AY3 712 88), PtTOC1 (NW_0 014 927 41) , and VvTOC1 (CAO64 513 ) For phylogenetic confirmation of TOC1 sequence ... regulates the morning expression of CIRCADIAN CLOCK ASSOCIATED (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) [15 18] The central role of TOC1 has been genetically con...

Ngày tải lên: 10/08/2014, 09:20

... equivalences between mouse and human cDC subsets [6] pDCs, a cell type discovered recently in both human and mouse, appear broadly different from the other DC subsets to the point that their place within ... levels in NK cells and all DC subsets; Itgam (Cd11b) in myeloid cells, NK cells, and CD11b cDCs; Ly6c at the highest level in pDCs but also strongly...

Ngày tải lên: 14/08/2014, 08:20

... STRUCTURAL INSIGHTS INTO PLANT CELL PROLIFERATION DISTURBANCE BY AGROBACTERIUM PROTEIN 6B WANG MEIMEI A THEIS SUBMITTED FOR THE DEGREE OF MASTER ... indicate protein colocalization 27 Chapter Results 3.1 Expression and purification of 6b proteins Before protein expression, a sequence comparison between the three 6b protein (AK6b, AB6b and T6b) ... Sequence alig...

Ngày tải lên: 13/10/2015, 16:50