biotransformation by plant cell cultures

... changes in (soluble and cell wall-bound) phenolic and stilbene concentrations during defence response in Norway spruce embryogenic cell suspension cultures induced by intracellular protein and wall ... ester-bound (SE), cell wall ester-bound (SWE) and soluble glycoside-bound (SG) phenolic acids in control cells of embryogenic cultures of Norway spruce (C) and in the cells elicited by mycelium protein ... (Medicago sativa L.) cell cultures in relation to their embryogenic ability Plant Cell Reports, 10: 251–255 Cvikrová M., Malá J., Hrubcová M., Eder J (2006): Soluble and cell wall-bound phenolics...

Ngày tải lên: 07/08/2014, 10:21

... The experiments were carried out in L-929 cell cultures (Tab.1) [6-9] The L-929 cell cultures were seeded in 96-wells After becoming confluent, the cells were treated with three different types ... of bone cells Besides the differently treated cells also untreated cells were measured as a reference Further glutaraldehyde, a toxic chemical substance, was added to the L-929 cell cultures ... diffuses into the cell culture medium Glutaraldehyde induces the dying of cells and therefore the consumption of oxygen by living cells decreases and the share of oxygen in the cell medium increases...

Ngày tải lên: 11/08/2014, 23:22

... a phytopathogen by virtue of its ability to transform normal plant cells into tumor cells This eventually results in tumor formation at wound sites of infected dicotyledonous plants During tumor ... attaches to plant cells and then transfers part of its tumor-inducing (Ti) plasmid to some of these plant cells The transferred T-DNA region (the segment of DNA that is surrounded by 24 basepair ... (an auxin) and isopentenyl-AMP (a cytokin) These plant hormones cause the tumorous phenotype that is characterized by the ability of the plant cells to proliferate limitlessly and autonomously...

Ngày tải lên: 13/10/2015, 16:50

... functional status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... productivity; (4) establishment of cell suspension cultures; (5) analysis of metabolite levels in cell suspension cultures; (6) selection of cell lines based on single cells; (7) analysis of culture ... capacities within cultures This has been achieved either by selection or by screening germplasm for highly productive cell lines, as for example, in production of Taxol from Taxus cell cultures (Kim...

Ngày tải lên: 25/10/2013, 05:20

... tested for homogeneity of variance by the loganova test and for normality by the Kolmogorov–Smirnov test for goodness of fit Significance levels of tests are indicated by asterisks according to the ... acclimation The effect of pH on the uptake of metals by the midge larvae is not only determined by the effect on chemical speciation but also by physiological effects At all exposure conditions ... given by: [X]T = [XH]+ [X]+ [XMe] (3) which, if combined with the expressions for the equilibrium constants in Eq (1) and (2) and assuming that only a small fraction of the sites is occupied by...

Ngày tải lên: 16/03/2014, 00:07

... participation of B cell- specific factors in pre-mature B -cell apoptosis mediated by BCR stimulation by analyzing the E2A-deficient DT40 mutant E2A)/), we clarified the impact of E2A on apoptotic cell death ... 1420 cell- cycle progression and proliferation of DT40 and E2A)/) cells Cells cultured in the presence of PMA/ ionomycin for 24 h or etoposide for h were analyzed by fluorescence-activated cell ... the DT40 cell line mediated by PMA/ionomycin treatment, but also that their own transcription is not influenced by PMA/ionomycin Therefore, the apoptotic cell death of DT40 mediated by PMA/ionomycin...

Ngày tải lên: 16/03/2014, 04:20

... –Pi cell culture being resupplied with 2.5 mM Pi All subsequent AP purification Concentration of culture media proteins secreted by –Pi cells was facilitated by Mini-Tan ultrafiltration followed by ... response of animals and plants [24,26] Several reports have documented the identification and partial characterization of APs from –Pi tomato plants or suspension cell cultures [10,11,27] Tadano ... hydrolases for tomato seedlings and suspension cell cultures A 57-kDa AP was partially purified from the cell culture medium of 3-day-old –Pi tomato suspension cells [10], but a thorough kinetic and structural...

Ngày tải lên: 23/03/2014, 21:20

... Microscopy of Plant Cells Introductory Survey Plant Cell (1): Light Microscopy Plant Cell (2): Overview by Electron Microscopy Plant Cell (3): Ultrastructural Details Plant Cell (4): Cell Surface ... of plant cell biology has been greatly enhanced by the spectacular advances wrought by the "molecular revolution" Students of plant science now have new and powerful tools for exploring plant cells, ... of plant cell biology has been greatly enhanced by the spectacular advances wrought by the "molecular revolution" Students of plant science now have new and powerful tools for exploring plant cells,...

Ngày tải lên: 08/04/2014, 12:52

... below the equilibrium value N, = Vcellcext(3 740) Here N, is the number of molecules taken up by a single cell, Vcell is the cell volume, and text is the extracellular concentration in a large ... GMDs, and then analyzed by flow cytometry so that the subpopulation of viable cells can be determined (4.5,46) Cellular stress caused by electroporation may also lead to cell death without irreversible ... volumetric ratio: ~“01 = (~extracellular/~~ntracellular) (9) may correlate with cell death or survival (47) According to this hypothesis, for a given cell type and extracellular medium composition,...

Ngày tải lên: 11/04/2014, 10:10

... ease with which plant material can be sterilized IS directly related to the way the plants providing the explants have been grown Diseased plants or plants that have been attacked by biting or sucking ... Protocols, edned by DaudLombej/ 1999 II3 DNA Repair Protocols EukarwrcS~ qlemp edlted by Do!I~/ S Hendeerw 1999 II2 2-D Proteome Analysis Prolocals, edlted by AndIe\ J Lml 1999 III Plant Cell Culture ... edlted by Smon R Swmdell, 1997 69 cDNA Library Protocols, edoed by Inn G Cone// and Carolrne A Ausrm 1997 68 Gene Isolation and Mappmg Protocols, edlted by Jocquebrw Eoulrwood, 1997 Plant Cell...

Ngày tải lên: 26/05/2014, 13:08

... Subconfluent cultures were split 1:3 When required, cell number was calculated by counting harvested cells using a hemocytometer chamber Cell viability and cellular proliferation Passage 2–4 cells were ... HUVEC cultures (4.5 ± 2.2 and 1.2 ± 0.4%, respectively; p = 0.005) Several growth parameters of the different cell cultures were analyzed Figure shows the cell proliferation kinetics of the cell ... umbilical artery endothelial cell cultures Proliferation fraction of exponentially growing and confluent human umbilical artery endothelial cell cultures Ki67 was detected by indirect immunofluorescence...

Ngày tải lên: 18/06/2014, 15:20

... re-stimulation with LCL, CD4+ T cells were adjusted to 1.5 × 106 cells/ml and CD8+ T cells to × 106 cells/ml Cytotoxicity assays Cytotoxic activity of CD4+ and CD8+ T cells was determined in a standard ... slightly modified the protocol by adding to the cultures IL-7, IL-15 or IL-21 instead of IL-2 Moreover, we separated and maintained in parallel cultures CD4+ and CD8+ T cells to better discriminate ... 96-well plates at doubling dilution, starting from 104 cells/well to 78 cells/well T cells were added to half of the replicates at 104 cells/well in HS complete medium without IL-2 Plates were...

Ngày tải lên: 18/06/2014, 16:20

... assessed and quantified by commercial ELISA Squares indicate DMSO only treated cultures and triangles indicate compound treated cultures The y-axis is OD450 nm The data were analyzed by two-way ANOVA ... 1,000 ms Cell viability assay Cell viability assays for compound toxicity were set up similar to compound screening except cells were not infected over the course of the experiments Cell viability ... units measured by IF 30000 20000 rlu ment of activity and toxicity in cell culture are prerequisite to lead compound development [4] Therefore, development of screening assays in cell lines that...

Ngày tải lên: 20/06/2014, 01:20

... differentially-stained cell cultures were documented by phase contrast microscopy using Olympus imaging software cell (Olympus, Hamburg, Germany) and quantified by counting Page of 12 Cells from F344 ... http://www.jeccr.com/content/30/1/102 a) F344 cells (P0) Page of 12 b) Lewis cells (P0) 100 μm 100 μm d) Lewis cells (P1) c) F344 cells (P1) 10 μm 10 μm Figure Differences in cultures of primary mammary cells from F344 and ... decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not altered...

Ngày tải lên: 10/08/2014, 10:21

... live and dead cells as well as total attached cells, assessed by Live/Dead fluorescent labelling Data represent the mean ± SE of three separate experiments from cells of different cultures Statistically ... attached and poorly attached cells, while the cell attachment assay using Live/Dead staining, a method involving rinsing of cell cultures, mainly measured firmly attached cell attachment and viability ... CdSe QDs on the cells decreases [11,12] Apoptotic epithelial cells are known to detach from growth substrates as well as neighboring cells [29] The detachment of Caco-2 cells from cell culture substrates...

Ngày tải lên: 11/08/2014, 00:22

... priority over CT synthesis for cellular carbon in salicyl alcohol-fed cells Whether salicyl alcohol feeding reduced cellular uptake of sucrose, thereby limiting cellular resources and CT accrual ... accrual by ~2 days At 10 mM, cells showed a downward trend of CT levels and did not resume CT synthesis throughout the 96 h feeding period At this feeding level, cell growth was also reduced by up ... in cultured cells and was significantly up-regulated by salicyl alcohol (Fig 6) The expression of MRP4 and MRP6 was lower in unfed cultures, but was also significantly upregulated by salicyl alcohol...

Ngày tải lên: 12/08/2014, 03:21

... hRz-transduced C6/36 cells The CPE of DENV-2 NGC infection in C6/36 cells, characterized by syncytium formation and decreased cell proliferation, was clearly visible days post infection (dpi) Those cells expressing ... transduced cells, made detection of hRz A B Figure RT-PCR2of total RNA extracted from hRz-C6/36 cells RT-PCR of total RNA extracted from hRz-C6/36 cells (A) hRz expression in the cells was detected by ... C6/36 control cultures (Fig 4C) These analyses confirm that hRz-C6/36 cell lines # 2, 5, and 11 suppressed the replication of DENV by at least 25% relative to the infected wild-type cells In vitro...

Ngày tải lên: 12/08/2014, 04:21

... in plants Plant Physiol 2002, 128:1031-1045 Chuong S, Park NI, Freeman M, Mullen R, Muench D: The peroxisomal multifunctional protein interacts with cortical microtubules in plant cells BMC Cell ... within living plant cells J Exp Bot 2000, 51:865-871 Köhler RH, Zipfel WR, Webb WW, Hanson MR: The green fluorescent protein as a marker to visualize plant mitochondria in vivo Plant J 1997, ... genome Plant Cell Physiol 2004, 45:590-599 Reichelt S, Knight AE, Hodge TP, Baluska F, Samaj J, Volkmann D, Kendrick-Jones J: Characterization of the unconventional myosin VIII in plant cells...

Ngày tải lên: 12/08/2014, 05:20

... in M truncatula cell cultures [9] In opium poppy cultures, cellular pools of GABA increased to maximum levels between 10 and 50 h after elicitor treatment Plant GAD is regulated by Ca2+/ calmoldulin ... poppy cell cultures Phytochemistry 1998, 98:136-138 Eilert U, Kurz WGW, Constabel F: Stimulation of sanguinarine accumulation in Papaver somniferum cell cultures by fungal elicitors J Plant Physiol ... synthetase in tomato leaves infected by the bacterial pathogen Pseudomonas syringae Plant Cell Physiol 2004, 45:770-780 Cowan AK: Phospholipids as plant growth regulators Plant Growth Regul 2006, 28:97-109...

Ngày tải lên: 12/08/2014, 05:20