MESENCHYMAL STEM CELLS AS THERAPY AGAINST HUMAN GLIOBLASTOMA MULTIFORME

... blue-stained cells over total cells (blue-stained cells + unstained cells) multiplied by 100 2.9 Caspase activity assay Caspase activity was determined using ApoAlert Caspase Colorimetric Assay Kit ... Expression level of caspase also plays a role in determining the sensitivity to TRAIL Increase in caspase degradation, as well as silencing of caspase through methylation, has been r...

Ngày tải lên: 01/10/2015, 17:27

... were harvested during total Table Histological scoring system for cartilage repair Category Points Cell morphology Hyaline cartilage Mostly hyaline cartilage Mostly fibrocartilage Mostly non -cartilage ... necessary, thereby increasing the safety and economic feasibility Our study will advance and extend the clinical application of MSC-based cell therapy for cartilage injury Ma...

Ngày tải lên: 09/08/2014, 10:23

... gene signaling pathway analysis has identified gene signals that are remarkably activated in AT-MSC-Hepa, and these signals are also up-regulated in whole liver Therefore, the microarray analysis ... protocol, and then treated with deoxyribonuclease (DNase I, amplification grade; TaKaRa, Kyoto, Japan) Microarray analysis and data mining (Aligent array) A one-color microarray...

Ngày tải lên: 30/03/2014, 04:20

... streptomycin (Invitrogen, Carlsbad, CA) in plastic flasks (25 cm2), and maintained in a humidified atmosphere of 5% CO2 in air at 37°C The culture medium used for expansion was initially changed ... hFTs lineage in passage two Panel B) Results of hFTs lineage in passage 11 We observed high rates of cell division, with gradual decreasing of the population doubling time (PDT...

Ngày tải lên: 18/06/2014, 15:20

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Ngày tải lên: 07/08/2014, 18:21

... 19 The relative increase in expression of the osteogenic markers in the young donor, as a result of chordin knockdown, was within the range of that of the donors over 70 years old MSCs from young ... analysis of flow cytometry data shows the transfection efficiency of siRNA The darker peak represents the fluorescence-positive cell population, which is clearly sh...

Ngày tải lên: 09/08/2014, 10:23

... antibodies used in 3D immunohistological and CD marker studies Antibody Source Dilution Type I collagen Biodesign International (Kennebunk, ME) 20 μg/ml Type II collagen Biodesign International (Kennebunk, ... dimensional; CD, cluster of differentiation plastic adherence and lineage specific differentiation satisfy the standard criteria suggested for defining mesenchymal...

Ngày tải lên: 09/08/2014, 10:23

... embryogenesis and morphogenesis of various tissues and organs, where they regulate growth, differentiation, chemotaxis and apoptosis of a variety of cell types, including mesenchymal, epithelial, ... compare the effects of BMP-4 and BMP-2 expression on chondrogenesis of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influ...

Ngày tải lên: 09/08/2014, 14:22

... 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating ... of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphoryla...

Ngày tải lên: 10/08/2014, 05:21

... Dou KF, Yang GS, Willerson JT, Geng YJ: Combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells increases benefits in infarcted swine hearts Arterioscler Thromb Vasc Biol ... neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplan...

Ngày tải lên: 10/08/2014, 05:21

... Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions Journal of Biomedical Science 2011 ... have successfully produced both neonatal cardiac myocyte and cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stem cells...

Ngày tải lên: 10/08/2014, 05:21

... this article as: Yang et al.: High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript ... differentiation of cryopreserved human adipose-derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of...

Ngày tải lên: 10/08/2014, 10:20

... cardiac-specific proteins in differentiated cells from all passages, as compared to untreated cells (data not shown) Nonetheless, cells in three-dimensional culture in medium containing insulin, dexamethasone ... properties, suppressing the activity of a broad range of immune cells, including N -cells, Bcells, natural killer cells and antigen presenting cells Transplantatio...

Ngày tải lên: 11/08/2014, 07:21

... WS: Intratracheal transplantation of human umbilical cord blood derived mesenchymal stem cells dose-dependently attenuates hyperoxia-induced lung injury in neonatal rats Cell Transplant 2011 Cordero ... umbilical cord blood-derived mesenchymal stem cells attenuate hyperoxia-induced lung injury in neonatal rats Cell Transplant 2009, 18:869-886 Chang YS, Choi SJ,...

Ngày tải lên: 12/08/2014, 13:22

... using the corresponding inhibitors They included 1-MT (1 mM), an inhibitor of IDO enzymatic activity, INDO (5 μM), an inhibitor of PGE2 synthesis, L-NAME (1 mM), a specific inhibitor of NO synthase, ... Notably, delayed addition of UC-MSCs maintained such inhibitory effects, suggesting that the transplantation of these cells is practicable and effective for treatment of RA...

Ngày tải lên: 12/08/2014, 15:22