automated adherent human cell culture mesenchymal stem cells

... 22 23 24 25 26 Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, ... A, Sekiya I: Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum Arthritis ... After to days, the medium was changed to remove nonadherent cells, and the adherent cells were cultured for days as passage without refeeding The cells were then trypsinized, harvested and resuspended...

Ngày tải lên: 09/08/2014, 10:23

... stem cells from whole human umbilical cord tissue J Cellular Biochem 2009, 107:926-932 Yang C-C, Shih Y-H, Ko M-H, Hsu S-Y, Cheng H, Fu Y-S: Transplantation of Human Umbilical Mesenchymal Stem Cells ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem ... Pluripotency in Human Embryonic Stem Cells Stem Cells Dev 2008, 17:1195-1206 Rodgers A, Mormeneo D, Long JS, Delgado A, Pyne NJ, Pyne S: Sphingosine 1-Phosphate Regulation of Extracellular Signal-Regulated...

Ngày tải lên: 10/08/2014, 05:21

... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat ... adipose-derived mesenchymal stem cells; MSCs: mesenchymal stem cells; ALP: alkaline phosphatase; DT: doubling time; EGM2-MV: endothelial cell growth medium 2; FACS: fluorescein-activated cell sorting; ... Induced endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation...

Ngày tải lên: 10/08/2014, 10:20

... tolerance with human adipose-derived mesenchymal stem cells Arthritis Rheum 2009, 60:1006-1019 Zheng ZH, Li XY, Ding J, Jia JF, Zhu P: Allogeneic mesenchymal stem cell and mesenchymal stem cell- differentiated ... reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans Stem Cells 2009, 27:1421-1432 Zappia E, Casazza S, Pedemonte E: Mesenchymal stem cells ameliorate experimental autoimmune ... A: Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies Mech Ageing Dev 2008, 129:163-173 Rao MS, Mattson MP: Stem cells and aging: expanding...

Ngày tải lên: 12/08/2014, 15:22

... Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells ... research 1.5.2.1 Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed to be ... has since been believed that hematopoietic stem cells may not differentiate into cardiomyocytes45 1.5.2.1.2 Mesenchymal stem cells Mesenchymal stem cells (MSCs) encompass a heterogeneous population...

Ngày tải lên: 12/09/2015, 09:42

... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells ... the effect of stem cells, or stem cell- conditioned media (CM), on disc cells Previous experiments with disc cells have shown that co -culture of nucleus pulposus with annulus fibrosis cells stimulated ... disc cells in the 3D culture after two weeks When disc cells were cultured alone, the CFSE label was clearly visible in almost all cells Both labelled and unlabelled cells were visible in co-cultures...

Ngày tải lên: 09/08/2014, 10:23

... from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells ... Epithelial mesenchymal transitions: twist in development and metastasis Cell 118, 277–279 31 Zipori D (2004) Mesenchymal stem cells: harnessing cell plasticity to tissue and organ repair Blood Cells ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell...

Ngày tải lên: 30/03/2014, 04:20

... Morphology of adherent cells when isolated from hFTs (primary cultures) A): Cells cultured for three days after initial plating Cells with an MSC-like phenotype and a small cluster of cells with ... Multipotent stem cells from umbilical cord: cord is richer than blood! Stem Cells 2008, 26(1):146-150 Zhang MJ, Liu B, Xia W, Sun ZY, Lu KH: Could cells from menstrual blood be a new source for cell- based ... T, Sakamoto M, Ogawa S, Umezawa A: Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells Stem Cells 2008, 26:1695-1704 Nasef A, Zhang YZ, Mazurier C, Bouchet...

Ngày tải lên: 18/06/2014, 15:20

... tnalugaocitna esortxed -etahpsohp-etartic deniatnoc taht )aeroK ,ssorC neerG( sgab noitcelloc doolb dradnats lm 052 otni detcelloc saw doolb ehT senilediug lanoitutitsni eht ot gnidrocca )aeroK ,luoeS( ... ytisrevinU lanoitaN luoeS ta seireviled mret -erp dna mret-lluf lamron morf deniatbo erew sllec BCU noitcelloc lleC sdohteM dna slairetaM la te kraP ooS-iK 443 dna snoisnetxe ralop-itlum gnol sa hcus ... scitsiretcarahc dna ygolohprom eht deyalpsid taht sllec tnerehda fo serutluc ot esir evag snoitcelloc doolb droc eht fo %04 yletamixorppa ,tsriF serutaef gniwollof eht yb deziretcarahc saw hcae...

Ngày tải lên: 07/08/2014, 18:21

... ‘bone marrow stem cell , which is often used for either hematopoietic or mesenchymal stem cells, the central nervous system stem cell, the hepatic stem cell, etc.? If many stem cells are found ... and regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can be maintained ... three main cell types: endothelial cells, hematopoietic stem cells, and stromal cells In a ground-breaking study, Friedenstein et al [30] isolated cells, clonogenic fibroblast precursor cells (CFU-F),...

Ngày tải lên: 09/08/2014, 01:21

... of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells ... between 100 and 1,000 cells/ cm2, trypsinised and stored in Recovery Cell Culture Freezing Medium in liquid nitrogen Before osteogenic differentiation, 6,000 cells/ cm2 of human MSCs were seeded ... of MSCs MSCs were cultured in BM or OM for 20 days The usual change in phenotype of MSCs from spindle-shaped cells (when cultured in BM) to more cuboidal-shaped cells (when cultured in OM) was...

Ngày tải lên: 09/08/2014, 10:23

... of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells ... label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, 6-CFDA negative), and viable cells (Ann5 ... with human mesenchymal stem cells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells...

Ngày tải lên: 09/08/2014, 14:22

... size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells induced ... of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... cells on supporting endogenous cells to regenerate However, the potential of bone marrow stem cells or MSCs to differentiate into IPCs has been demonstrated in vitro and in vivo Bone marrow cells...

Ngày tải lên: 10/08/2014, 05:21

... of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... from mutated human embryonic bone marrow mesenchymal stem cells Oncol Rep 2004, 12:501-508 22 Lee HJ, Choi BH, Min BH, Park SR: Changes in surface markers of human mesenchymal stem cells during ... staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle-like morphology...

Ngày tải lên: 10/08/2014, 10:20

... medium, and the cells were seeded in 10 cm culture dishes After 24 hours, non adherent (hematopoietic) cells were removed by medium change Mesenchymal stem cells were expanded in stem cell culture medium ... (about 10,000 cells/ cm2), stem cells were trypsinized with stem cell trypsin (CellSystems, St Katharinen, Germany) and re-plated at a density of 1000/cm2 to expand cell culture (cell proliferation ... properties, suppressing the activity of a broad range of immune cells, including N -cells, Bcells, natural killer cells and antigen presenting cells Transplantation of MSCs into the periinfarct area decreases...

Ngày tải lên: 11/08/2014, 07:21

... activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic T -cell ... Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, ... 11 Mesenchymal stem cell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells...

Ngày tải lên: 12/08/2014, 11:23

... Neyrinck AP, Matthay MA, Lee JW: Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1 J Biol ... undifferentiated mesenchymal stem cells Exp Hematol 2003, 31:890-896 Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC: Suppression of allogeneic T -cell proliferation by human marrow stromal cells: ... Lee JW, Matthay MA: Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37 Stem Cells 2010, 29:2229-2238 11 Rocha V, Wagner...

Ngày tải lên: 12/08/2014, 13:22

... canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) we differentiated ... concentration of 500 μM and stored at -80°C Serial dilutions were prepared in culture medium Cell culture Mesenchymal stem cells (MSCs) were isolated from canine adipose tissue biopsies and primary ... of human bone marrow mesenchymal progenitor cells J Cell Physiol 1999, 181:67-73 20 Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP: Osteogenic differentiation of purified, culture- expanded human...

Ngày tải lên: 12/08/2014, 14:22

... disc cell- mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells ... F, Mochida J: Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell- to -cell contact with autologous mesenchymal stem cells J Orthop ... disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 11 Yoshikawa T, Ueda Y, Miyazaki K, Koizumi M, Takakura Y: Disc regeneration therapy using marrow mesenchymal cell transplantation:...

Ngày tải lên: 12/08/2014, 15:23

... of amniotic fluid-derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stem cells and their use in cell- based terapies Cancer ... Jorgensen B, Feldmann S, Ehringer G, Bornhauser M, Werner C: Mesenchymal stem cells can be differentiated into endothelial cells in vitro Stem Cells 2004, 22:377-384 47 Chelucci C, Federico M, Guerriero ... direct staining of cell cultures with red oil The microscopic evaluation of the red oil stained cell cultures showed a reliable increase in red oil stained cells in the cell cultures treated with...

Ngày tải lên: 13/08/2014, 01:20