ex vivo expansion of human mesenchymal stem cells on microcarriers

... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells into ... development to full expression of the adult skeleton a continual slow transition occurs from woven bone to lamellar bone Endochondral ossification occurs at extremities of long bones, vertebrae, ... alizarin red or von Kossa 1.7 In vivo bone formation using Stem Cells Bone formation through in vivo implantation of the stem cells that are directed to osteogenic differentiation are being studied...

Ngày tải lên: 09/10/2015, 11:24

... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor ... isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T-cell proliferation; design of ... suppression of T cell proliferation through up-regulation of B7-H1 Cell Res 2008, 18:846-857 13 Glennie S, Soeiro I, Dyson PJ, Lam EW, Dazzi F: Bone marrow mesenchymal stem cells induce division arrest...

Ngày tải lên: 12/08/2014, 11:23

... Kaplan DL 2005;Matrix-mediated retention of adipogenic differentiation potential by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion Biomaterials (31):61676175 83 Mariman ... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... differentiation of rat bone marrow-derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells...

Ngày tải lên: 11/09/2015, 09:16

... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J ... expression, whereas the control siRNA cells exhibited detectable chordin mRNA expression (Figure 2b) Knockdown of chordin expression in human MSCs Effects on morphology and proliferation There...

Ngày tải lên: 09/08/2014, 10:23

... expression To examine further the effects of BMP-2 and BMP-4 gene delivery on hypertrophic differentiation, we analyzed the temporal expression profiles of genes associated with chondrocyte maturation ... MF: Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ... Hentunen T, Laitala-Leinonen T, Saamanen AM: Impact of stromal cell composition on BMPinduced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells Exp Cell Res 2008, 314:2400-2410...

Ngày tải lên: 09/08/2014, 14:22

... derived from mesenchymal stem cells in Wharton’s Jelly of the human umbilical cord for transplantation to control type diabetes PLoS ONE 2008, 3(1):e1451 Liu M, Han ZC: Mesenchymal stem cells: biology ... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... Human bone marrow mesenchymal stem cells can express insulin and key transcription factors of the endocrine pancreas developmental pathway upon genetic and/or microenvironmental manipulation in...

Ngày tải lên: 10/08/2014, 05:21

... native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, the importance of nanofibers ... biodegradable nanofibers mimicking native bone ECM can enhance the osteogenic differentiation of mesenchymal stem cells and promote bone formation By culturing human MSCs on PLLA electrospun nanofibrous ... nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells on the nanofibers were significantly higher than on the controls...

Ngày tải lên: 09/09/2015, 10:07

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Classification of stem cells Germ cells of the gonads Mammalian stem cells are usually classified according to their tissue of origin The ovary and testis contain oogonia and spermatogonia which ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells...

Ngày tải lên: 16/09/2015, 15:55

... expansion of NK cells IL-2 stimulation of PBMCs was shown to elicit absolute expansion of NK cells and CD56+ T cells, e.g NKT cells, γδ T cells and some αβ/CD8+ T cells [30] The combination of ... related expansion of CD4+/CD25+/ CD127-/FoxP3+ regulatory T cells IL-2 is critical for the ex- vivo expansion of suppressive regulatory cells [25] Using high doses of IL-2 in our NK cell expansion ... reduction of the expansion of CD4+ T cells At the donor level, a higher proportion of NK cells tend to be associated, in absence or in presence of 3aG1, with a low proportion of CD4 T cells within...

Ngày tải lên: 18/06/2014, 15:20

... Differentiation of hfMSC .141 6.4.3 Effects of Hypoxia precondition on Retaining hfMSC Properties 144 6.4.4 Ex Vivo Expansion in Hypoxiacontinuous on hfMSC Growth Kinetics 146 6.4.5 Ex Vivo Expansion ... Y Liu, SH Teoh Effects of Strontium on the Proliferation and Bone Forming Capacity of Human Fetal Mesenchymal Stem Cells Seeded onto Scaffolds International Conference on Cellular & Molecular ... bones (Clarke 2008) 2.2 Anatomy of Bone The human skeletal system consists of 80% cortical bone and 20% spongy bone overall, with varying ratios depending on the skeletal site and type of bone...

Ngày tải lên: 09/09/2015, 10:16

... boundaries, and with weak expression of SSEA3 and TRA-1-81 A total of 200 colonies were examined for each experimental group, as well as for the control Statistical comparison of data was performed ... for extended durations Spontaneous differentiation of hESC after exposure to low temperature Following serial passage after exposure to low temperature, the proportion of Grade A, B and C colonies ... WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human...

Ngày tải lên: 31/10/2012, 16:57

... of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration ... long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube ... and human embryonic stem cells derived These breakthroughs paved the way to the derivation of the first five human embryonic stem cell lines by Thomson et al in Madison, Wisconsin, 1998 (Thomson...

Ngày tải lên: 22/03/2014, 09:20

... technical platforms used in assessing miRNA expression Gene expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples ... cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression ... level of non-target genes of the same number was also correlated with the miRNA expression Density plot of correlation coefficient distribution was generated in R environment Validation of differentially...

Ngày tải lên: 18/06/2014, 15:20

... areas were considered to be ON+ , while those without ON lesion were considered to be ON- The ON+ rabbits were further divided into sub-single-lesion group (SON group: with one ON lesion) and sub-multiple-lesion ... incidence and extension No rabbits died during the entire experiment period Of the 25 rabbits, 15 were found ON+ (60%) and 10 were ON- (40%) Of the 15 ON+ rabbits, rabbits had only one ON lesion and ... sub-multiple-lesion group (MON group: with more than one ON lesion) Statistics The differences between ON+ and ON- group, MON and MON group were analyzed by nonparametric MannWhitney test using SPSS software...

Ngày tải lên: 20/06/2014, 00:20

... the exact involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo ... is unclear whether loss of Stro-1 expression alone has functional consequences for MSC stemness Application of Stro-1 as an MSC marker is therefore best done in conjunction with other markers (see ... vivo expression profiles of the responsible factors will shed light on when, where, and how MSCs migrate What is known is that injury alters the patterns of migration and differentiation of exogenously...

Ngày tải lên: 09/08/2014, 10:20

... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, ... cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3,...

Ngày tải lên: 11/08/2014, 12:21

... Umezawa A, Sekiya I: Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum Arthritis ... http://arthritis-research.com/content/12/6/R206 Page of 13 Figure Synovial MSCs affect gene profile of nucleus pulposus cells in co-culture system (a) Morphology of mono-culture of rat nucleus pulposus cells and human ... co-culture of rat nucleus pulposus cells with human synovial MSCs at one and seven days (b) Human gene profile of human synovial MSCs in mono-culture and in co-culture with rat nucleus pulposus cells...

Ngày tải lên: 12/08/2014, 15:22

... Richardson SM, Zeef LA, Freemont AJ, Hoyland JA: Characteriation of the human nucleus pulposus cell phenotype and evaluation of novel marker gene expression to define adult stem cell differentiation ... initiation and progression of chondrogenic differentiation of mesenchymal stromal cells in vitro Stem Cells Dev 2009, 18:881-892 42 Weiss S, Hennig T, Bock R, Steck E, Richter W: Impact of growth ... Minogue BM, Richardson SM, Zeef LA, Freemont AJ, Hoyland JA: Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral...

Ngày tải lên: 12/08/2014, 15:23

... level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells were obtained after optimization of the promoters and modulators Second, ... the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus vectors had no effects on the cell ... been done on the use of the human Oct4 promoter to drive transgene expression in hES cells One study has demonstrated the activity of an exogenous Oct4 promoter in driving the expression of eGFP...

Ngày tải lên: 11/09/2015, 14:38

... made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of several experimental approaches to ... Delta μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising ... liver could be the results of these colonisations and expansion of the population by the liver itself (Takeuchi et al 2002) Beyond this point the liver functions as the site of haematopoiesis until...

Ngày tải lên: 04/10/2015, 16:03