Novel biochemical and functional properties of the HPV 16 oncoprotein e6

... diagram of the HPV1 6 E7 protein Figure 5: Schematic diagram of the HPV1 6 E6 protein Figure 6: Expression and metal-affinity purification of HPV1 6 E7 Figure 7: Apparent size distribution of HPV1 6 E7 ... between the L1 and E6 ORFs (Figure 3) Figure 3: Organization of the genome of HPV 16 Most of the ORFs overlap and the early genes are all transcr...

Ngày tải lên: 17/09/2015, 17:18

... rather than on the position of the mutation in the polypeptide chain, as demonstrated by the biophysical assays Our studies have provided additional contributions to the understanding of the effects ... charge of Tm The two other mutations (A63V and K70T) are located at the N-terminal region, far from the troponin binding region and occur at the g positio...

Ngày tải lên: 16/03/2014, 18:20

... tokodaii; Ap, Aeropyrum pernix; Ta, Thermoplasma acidophilum; Tv, Thermoplasma volcanium; Fa, Ferroplasma acidarmanus; Tm, Thermotoga maritima; Aa, Aquifex aeolicus; Tt, Thermoanaerobacter tengcongensis ... presence of unlabeled ATP, indicating that ATP and the analog 8-azido-ATP recognize the same binding site The ATPase activity of PfPDO was demonstrated The hydrolysis of ATP...

Ngày tải lên: 16/03/2014, 16:20

... characterization of the human VGSC b1B subunit The human b1B subunit is a novel splicing variant of the b1 subunit via alternative intron retention The retained intron encodes a novel extracellular, ... peptide Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the hum...

Ngày tải lên: 16/03/2014, 23:20

... of the inner lining of the ion channel pore The N- and C-termini of TRP channels are located in the cytoplasm and, depending on the respective TRP channel, consist of various functional TRP channels ... Physical and functional interaction of other TRP channels with cytoskeletal components Physical links of several TRP channels other than TRPV...

Ngày tải lên: 30/03/2014, 10:20

... In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then attempt to relate this to the functional properties of these cells Figure shows the workflow and the way in which ... pooled and a cytokine bead array (CBA; Inflammation kit, Becton Dickinson) was performed in triplicate Endocytosis assay For the analysis of the endocytic activi...

Ngày tải lên: 14/08/2014, 18:20

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [H...

Ngày tải lên: 15/02/2014, 01:20

... that MCPIP regulates the amount of IL-1b mRNA Involvement of PIN domain of MCPIP in the stability of IL-1b mRNA In order to find out whether the PIN domain in MCPIP is responsible for IL-1b mRNA ... interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level...

Ngày tải lên: 18/02/2014, 14:20

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the heli...

Ngày tải lên: 18/02/2014, 18:20

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of t...

Ngày tải lên: 21/02/2014, 15:20

... histidines (His67 and His247, presumably providing metal binding at site II and the neighbouring His242) bind phosphate ions, thereby providing another level of competition for metal ion binding Kinetics ... modelling of Cu(II) and Ni(II) transport by albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at sit...

Ngày tải lên: 22/02/2014, 04:20

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal bioge...

Ngày tải lên: 07/03/2014, 16:20

... al Selective effects on the reduced state of HRPC Determination of dissociation constants for benzhydroxamic acid The dissociation constants (Kd) of complexes formed between resting state enzymes ... selective effects that are mediated exclusively on the reduced state of the enzyme We hypothesize that this residue imposes a degree of rigidity to th...

Ngày tải lên: 07/03/2014, 21:20

... rans phil us Vibrio cholerae Escherichia coli Rickettsia prowazekii Sulfolobus acidocaldarius Aquifex aeolicus Helicobacter pylori B ac Prokaryotic Family I sPPases * Neisseria meningitidis 925 ... (MDLSRIPPQP KAGILNVLIE IPAG), and Rhodop viridis (MRIDA IDXA), and that of Mi aeruginosa NIES-44 (MDL SRKPAQP IPGLKNVLVE TAGSINIT) [16], show a high degree of similarity with other cyto...

Ngày tải lên: 16/03/2014, 11:20

... like the Trp residue of the 183 Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and ... ligand binding and distribution of nematode protein Ag-NPA-1 Fig Immunogold electron microscopic localization of Ag-NPA-1 in a male A galli worm (A) Sect...

Ngày tải lên: 16/03/2014, 18:20