biochemical nutritional and functional properties of kimchi

... (PART 1): BIOPHYSICAL PROPERTIES OF E6 AND E7 33 3.1 Biophysical properties of the E6 and E7 proteins: Previous data and concept of this study 33 3.2 Biophysical properties of the E7 protein: ... properties of E6 and E7 3.1 Biophysical properties of the E6 and E7 proteins: Previous data and concept of this study To determine the biophysical properties of proteins and, ultimately, determine their ... casein kinase II and the location of the zinc binding domain are indicated The HPV E6 protein consists of two tandem copies of this domain and it has been speculated that E6 and E7 may have evolved...

Ngày tải lên: 17/09/2015, 17:18

... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the MCPIP by use of bioinformatics, ... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands)...

Ngày tải lên: 18/02/2014, 14:20

... oxygen-binding properties The ultimate aim is a precise definition of the allosteric unit responsible for cooperativity and of the possible role of heterogeneity at subunit level in the modulation of the functional ... allosteric properties of the protein and to trace the evolutionary pathway of the allosteric behaviour Results Characterization of the oligomeric state of P monodon Hc In gel-filtration experiments of ... structural stability of arthropod Hc oligomers, the reversibility of the dissociation processes and the occurrence of different subunits, in order to correlate the structural properties of the various...

Ngày tải lên: 23/03/2014, 13:20

... activation Several functional properties of TIMPs are of potential importance in the pathogenesis of arthritis Overexpression of TIMP-1 and TIMP-2 is associated with inhibition of apoptosis and invasive ... laboratory, and statistical evaluation of material from RA patients; and preparation of the manuscript LD performed the collection and clinical analysis of patients with degenerative joint diseases and ... revision of the manuscript AT contributed to the conception of the study and study design, statistical evaluation of the results, and preparation of the manuscript All authors read and approved...

Ngày tải lên: 09/08/2014, 06:23

... between maternal and infant clones The V3 loop charge and glycosylation are predictive of co-receptor tropism [19,20] Examination of charge and glycosylation of the V3 loops of our env clones ... 88% of env clones proved functional, with no significant differences in functionality between mothers and infants detected within or across transmission pairs (data not shown) A total of 162 functional ... expression of relatively high levels of the CD4 receptor and CCR5 co-receptor on the surface of target cells [21,39] However, levels of CD4 and CCR5 on mucosal and submucosal cell subsets can be...

Ngày tải lên: 13/08/2014, 01:21

... mDCs and moDCs [12] and moDCs and KG-1 cells [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and ... presence of × 104 irradiated (3,000 rad 137Cs) immature mDCs and interactions Figure Cytokine expression profiles of cells Cytokine expression profiles of cells Cytokine production by mDCs and the ... The proteome of mDCs showed different levels of similarity compared with those of moDCs and KG-1 cells (correlation coefficient 0.68 and 0.62, respectively) (Figure 4) Duplicate gels of mDCs were...

Ngày tải lên: 14/08/2014, 18:20

... members, Professor Ng Huck Hui, Dr José   R   Dinneny, and Professor Toshie Kai for their valuable comments and constructive advice My sincere thanks to all the past and current members of the Cohen ... (representative reviews in (Iorio and Croce, 2012; Mendell and Olson, 2012; Rottiers and Naar, 2012)) My PhD project is focused on identification and functional characterizations of miRNA-target interactions ... the translation of lin-14 and lin-28 mRNAs, both of which encode two heterochronic regulators in C elegans Analysis of sucrose gradients and metrizimide-buoyant density gradients of these mRNAs...

Ngày tải lên: 09/09/2015, 10:17

... Rao and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS ... molecular mass of 19 216 Da and a pI of 4.69, in good agreement with MALDI-TOF MS (Fig 1A) and chromatofocusing data, respectively The other three N-terminal sequences determined in this work, of Anabaena ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary...

Ngày tải lên: 16/03/2014, 11:20

... contribution of the mutant amino acids to the Tm properties Functional properties of mutant tropomyosins Recombinant Tms were assayed by structural (head-to-tail polymerization and binding to actin) and ... microcalorimetric study of Tm denaturation was performed using a scanning microcalorimeter MicroCal Ultrasensitive VP-DSC and standard software for data acquisition and analysis Tm concentrations were of 15 lM ... cosedimentation assay, in the absence and in the presence of troponins In the absence of troponins, binding of Tms to actin was very weak and only small amounts of Tm were detected in gels after...

Ngày tải lên: 16/03/2014, 18:20

... A Biochemical properties of AnpAFP 396 D 116.25 97.4 66.2 C Figure shows the biochemical properties of AnpAFP SDS ⁄ PAGE (Fig 2A) followed by silver staining showed that the molecular mass of ... both AFPs had relatively low contents of Arg (1.3% and 0.9%), Met (0.8% and 0.9%) and Tyr (1.9% and 2.4%) Another significant feature of AnpAFP is the presence of 2% Cys; however, Cys was not detected ... indicative of the binding of these hyperactive AFPs to both pyramidal and basal planes of a seed hexagonal ice crystal Comparison of TH activity between AnpAFP and TisAFP8 Figure 5A shows the results of...

Ngày tải lên: 22/03/2014, 21:20

... presence of nocodazol or increased Ca2+ concentrations [28] TRPV1 channels are nonselective cation channels Therefore, the role of increased concentration of Ca2+ on the properties of TRPV1–tubulin and ... Transfection of TRPV1 in dorsal TRP channels and cytoskeleton regulate each other root ganglia-derived F11 cells results in co-localization of TRPV1 and microtubules and accumulation of endogenous ... stabilization of the microtubule results in the induction of neuritogenesis and the formation of elongated cells (C) Time series of a growth cone developed from F11 cell expressing TRPV1–GFP Application of...

Ngày tải lên: 30/03/2014, 10:20

... practice given the current volume of SNP data, and there have not been any large-scale studies of selective constraints on protein structural and functional properties using SNP data In the present ... alignments and protein alignment conservation We then obtained a variety of structural and functional properties from either database annotations or computational prediction methods and analyzed ... first base of the first complete codon, and the last base the last of the final complete codon The first and last codons were then removed from each exon, and remaining codons within 70 bp of the...

Ngày tải lên: 14/08/2014, 08:21

Ngày tải lên: 14/11/2014, 08:21

... properties of food proteins: effects of reduction of disulfide bonds on film properties and foam stability of glycinin Journal of Food Science 1987, 56, 242-258 27) Kinsella, J E Functional properties ... during the extraction of 11S caused the 11S to have higher level of isoflavone, better foaming properties and formed harder gels I The functional and structural properties of 2S soy protein in ... Table 1.2 Functional properties of soy protein (Kinsella, 1979) Table 2.1 Hardness of soy gel heated for 60, 80 and 100 minutes Table 2.2 Comparision of L*, hardness and pH of various ratio of 7S:11S...

Ngày tải lên: 16/09/2015, 08:30

... principles and actual devices Electronic and Optoelectronic Properties of Semiconductor Structures provides engineering and physics students and practitioners with complete and coherent coverage of key ... manual and set of viewgraphs for use in lectures is available for instructors   received his Ph.D from the University of Chicago and is Professor of Electrical Engineering and Computer ... University of Michigan, Ann Arbor He has held visiting positions at the University of California, Santa Barbara and the University of Tokyo He is the author of over 250 technical papers and of seven...

Ngày tải lên: 24/01/2014, 17:34

... the compensation of ion Fe3+ in the tetrahedral and octahedral location That led to increasing of total magnetization of a molecule Ni1-xCoxFe2O4 and hence there is increasing of MS (table 3) ... high coercivity of materials, the second term can take part of several tens percent of HC and the last one whereas can only reach several hundreds of gauss Thus, the increasing of Co-concentration ... Co-concentration led to the increasing of anisotropy property of ferrites Ni1-xCoxFe2O4, hence it was the increasing of HC The increasing of HC proved that the soft magnetic property of ferrite NiFe2O4 changed...

Ngày tải lên: 13/02/2014, 04:20

... tests of physical performance for assessing the functioning of upper and lower extremities were carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising ... 84% in Indonesia and 62% in Thailand (10) It was D N Fernando and R de A Seneviratna 14 Table Self-assessed "health status" by some psychosocial factors and indicators of use of health services ... American Journal of Public Health 1981; 71(11): 1211-1216 D N Fernando and R de A Seneviratna Fries J F Aging, natural death and the compression of morbidity Tlie New England Journal of Medicine 1980;...

Ngày tải lên: 14/02/2014, 06:20

... characterization of numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided into two domains: a core domain and ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7...

Ngày tải lên: 15/02/2014, 01:20

... mutant enzymes, F133N and F133A, were expressed, purified and characterized With mm UMP and ATP as substrates, the activities of F133N and F133A were only 50 and 20% of that of the wild-type enzyme ... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E A phosphate ion was found in the donor site of all subunits (Fig 2B),...

Ngày tải lên: 18/02/2014, 16:20

... Araujo Soares RM (2006) Secretion of serine peptidase by a clinical strain of Candida albicans: influence of growth conditions and cleavage of human serum proteins and extracellular matrix components ... antibacterial ⁄ fungicidal properties of trappin-2 involve the cationic nature of the molecule, as assessed from the salt and heparin dependence of the antimicrobial and antifungal effects Results ... fungicidal effect of a lm concentration of trappin-2 towards C albicans was time-dependent, reaching 92% after h and 98% after 18 h (Fig 5) Effect of NaCl and heparin on the antibacterial and antifungal...

Ngày tải lên: 18/02/2014, 17:20