2D DIGE analysis of butyrate treated hepg2 cells

... The number of migrated cells treated with butyrate was expressed as a ratio of migrated control cells Figure 4.3: 1-D western blot of p21, c-Myc, and p53 on mM, 24 hr butyrate- 69 treated (Trt) ... migrated treated cells are expressed as a ratio of corresponding control cells without butyrate treatment 11 INDEX OF ABBREVIATIONS 1-D One-dimensional 2-DE Two-dimensi...

Ngày tải lên: 15/09/2015, 22:42

... healthy individuals Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of 20% in samples of patients with AML and 13,7% in healthy individuals After ... Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line Nature 2001;413(6853):316-22 Yada M, Hatakeyama S, Kamura T, Nishiy...

Ngày tải lên: 31/10/2012, 16:49

... assay as a marker of classification of RNA- positive reticulocytes and erythrocytes 10, 11, 12 or by attempts to describe of human erythroid gene activity, planed to be finished in 5-10 years ... human RBCs The purity of erythrocyte fraction achieved 99,99997% and was confirmed by Pappenheim staining of blood slides, flow cytometry (MÖLAB, Germany) and FACS analysis (Cytomic...

Ngày tải lên: 03/11/2012, 10:58

... cRBCs Defining the glycans present on the surface of cRBCs will allow either for the design of strategies to optimize the agglutination assay or the design of alternative strategies for the detection ... measurement of various concentrations of solutions can then be used to quantify viral titer Additionally, the introduction of antisera capable of neutralizing...

Ngày tải lên: 14/02/2014, 19:20

... of bilitranslocase controls the permeability of HepG2 cells to bilirubin Results and Discussion Expression of bilitranslocase in HepG2 cells HepG2 cells were used to study hepatic uptake of bilirubin ... establish the role of bilitranslocase in organic anion uptake in HepG2 cells Bilirubin analysis by thermal lens spectroscopy The uptake of...

Ngày tải lên: 07/03/2014, 21:20

... a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose ... status in the presence and absence of insulin and ⁄ or TNFa Figure 2A, B shows the effects of TNFa pretreatment on insulin action on the lo...

Ngày tải lên: 16/03/2014, 02:20

... doi:10.1186/1479-5876-9-96 Cite this article as: Ponnikorn et al.: Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia Journal of Translational Medicine 2011 9:96 Submit your ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid...

Ngày tải lên: 18/06/2014, 19:20

... substantially due to induction of apoptosis This was supported by the observation of characteristic apoptotic cell morphology and phosphoproteomic analysis of HbE/b-thalassemia Our phosphoproteome data ... Ponnikorn et al.: Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia Journal of Translational Medicine 2011 9:96...

Ngày tải lên: 20/06/2014, 04:20

... profiles of PLGA-based nanoparticles transfected HepG2 cells Numbers in Table indicate the percentage of cells remaining in each phase of cell cycle (A) Untreated group; (B) miR-NC contained nanoparticles ... for gene transfer/therapy Mol Ther 2005, 11:990 doi:10.1186/1556-276X-6-447 Cite this article as: Liang et al.: PLGA-based gene delivering nanoparticle enhance...

Ngày tải lên: 21/06/2014, 02:20

... Res Lett (2008) 3:416–420 design can be employed as memory cells We also develop a continuum model in this paper to analyze the proposed NEMS memory cell design Design of Memory Cells Figure illustrates ... used as a random access memory (RAM) cell It should be noted that Kang and Hwang [16] proposed the similar NEMS design, called ‘Carbon nanotube shuttle’ memory device Ho...

Ngày tải lên: 22/06/2014, 01:20

... study examining the global proteome profile of the visceral leishmaniasis patients’ sera Here, we report, for the first time, the difference- in -gel electrophoresis (DIGE) analysis of sera from visceral ... Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients Lokesh A Rukmangadachar1,2, Jit...

Ngày tải lên: 13/08/2014, 13:20

... ethanol- regulated genes were involved using KEGG (Kyoto Encyclopedia of Genes and Genomes) [36] and GenMAPP (Gene Microarray Pathway Profiler) [37] analysis As shown in Table 2, only ITGB4 was found to be involved ... considering the reported high expression of the ankyrin-repeat oncoprotein (gankyrin) in human hepatocellular carcinoma Gankyrin binds to the cell-...

Ngày tải lên: 31/10/2012, 15:28

... before inoculation of C trachomatis Strain L2/Bu434 of C trachomatis was kindly provided by Dr P Saikku (University of Oulu, Finland) Chlamydial strains were initially propagated in Hep2 cells and ... HepG2 cells infected with C trachomatis in presence of mevastatin HepG2 cells were set up, grown and infected with C trachomatis in presence or absence o...

Ngày tải lên: 13/08/2014, 13:20

... molecules in the course of hypoxia-induced apoptotic pathways Such analysis of trophoblastic cells will, in the near future, yield insights into the mechanisms of preeclampsia and other hypoxia-related ... and by modulating phosphorylation of JNK In conclusion, various pro- and antiapoptosis-related proteins were expressed in the process of hypoxia-induced apoptos...

Ngày tải lên: 31/10/2012, 15:12