2d dige analysis and protein identification

... recordings with a Gould pressure transducer and recorder, and a submandibular gland dissected free In 10 BALB/c mice and 10 NOD mice (Group 1), the submandibular duct comprising the lingual nerve ... responses were measured and saliva collected, the submandibular gland used for blood flow measurement was removed and fixed in 10% buffered formalin and the contralateral gland was snap-frozen in ... 4c) Localization of M3R in submandibular glands Double labelling of CD31 and M3R revealed M3R in the wall of blood vessels in submandibular glands of both NOD mice and BALB/c mice (Figure 5a,b)...

Ngày tải lên: 09/08/2014, 01:22

... L-NAME + AlCl3- and L-NAME-treated) and control (shamoperated) animals (p ＜ 0.05) ∙Indicates a statistically significant difference between treated (L-NAME + AlCl3- and L-NAMEtreated) and AlCl3-treated ... LNAME + AlCl3- and L-NAME-treated) and control (sham-operated) animals (p ＜ 0.05) ∙Indicates a statistically significant difference between treated (L-NAME + AlCl3- and L-NAME-treated) and AlCl3-treated ... treated (AlCl3- and LNAME-treated) and control (sham-operated) animals (p ＜ 0.05) ∙Indicates a statistically significant difference between treated (L-NAME + AlCl3- and L-NAME-treated) and AlCl3-treated...

Ngày tải lên: 07/08/2014, 23:22

... fractions from sweet potato leaves The controls were ND and obese diabetic untreated mice 2.5 Blood and liver collection and biochemical analysis The blood of mice fasted for 12h was collected ... insulin and key hepatic enzymes in experimental diabetes, Pharmaceutical Biology 40(3), (2002) 165 [8] N Brandstrup, JE Kirk, C Bruni, The hexokinase and phosphoglucoisomerase activities of aortic and ... (18mmol/l) were considered to be diabetic and used in this study [4,6] 2.4 Treatment of obese and diabetic mice by extract fractions from sweet potato leaves The obese and diabetic mice were treated orally...

Ngày tải lên: 12/02/2014, 17:20

... Inhibition of enzymatic activity of the digestion and absorption of dietary fat such as lipase, lipoprotein lipase and enhancement of carnitine acyl transferases activity and fatty acid oxidation enzymes ... treatment and prevention of obesity and diabetes and other diseases The investigation of effects of plant extracts on activity of lipid-carbohydrate metabolic enzymes such as lipase, lipoprotein ... effective for treatment of human obesity and, possibly diabetes [2] Anti-hyperlipidemic and anti-diabetic effect of metformin were showed, but side-effect and its efficacy of remains in debate if...

Ngày tải lên: 14/03/2014, 10:20

... soil and Cu accumulation in plant, and also the role of phosphate in reducing the uptake of Cu by Brassica juncea L 2.2 Sampling and chemical analysis Plant and soil samples were taken and analysed ... samples (leaves and shoots) are collected and washed with pure water and then dried at 70oC until stabilisation of weight The monitoring indicators for plants growth include plant height and biomass ... height and biomass Total Cu in soil and plant tissues, Cu2+ (HNO3 0.43N) in soil was determined by Atomic Absorption Spectroscopy (AAS) Materials and methods Results and discussion 2.1 Pot experiments...

Ngày tải lên: 14/03/2014, 15:20

... 14 15 16 Authors' contributions EW and LAM conceived of and designed the study MJ and KAT recruited subjects for and organized the focus groups EW analyzed and interpreted the data All authors ... from each woman including age, race, employment and marital status, and number of children, and the Women's Experience with Battering Scale (WEB[20]) and a modified version of the Conflict Tactics ... relationship and ranges in score from 10 to 60 where 20 or higher is indicative of battering The CTS measures frequency and severity of physical, emotional and sexual abuse in both current and former...

Ngày tải lên: 18/06/2014, 22:20

... the special tests in random order for both object-centered and egocentric co-ordinates Patient A performed the common clinical test and two special tests; special test and and Patient B performed ... performed the common clinical test and four special tests; special test 3, 4, and Results In this study, the score of FIM-M and FIM-C of Patient A and B was 42 and 58, respectively The scores indicate ... substantial contribution to acquisition and analysis of the data T Ifukube and T Izumi were involved in conception and design of the study, interpretation of the data and revision of the manuscript for...

Ngày tải lên: 19/06/2014, 08:20

... distance and speed, stride length and duration, cadence, stance, swing duration, double support, and their respective CVs (if applicable) Gait parameters were averaged between the right and left ... speed (p < 0.04) and the CV of the stride interval (p < 0.02) differed between the groups and between the S-VS on and S-VS off conditions (Table 2) The walking speed increased and the CV of the ... experiments, data analysis, interpretation, and manuscript preparation V.N contributed to study design and participated in data analysis, interpretation, and manuscript preparation Acknowledgements...

Ngày tải lên: 19/06/2014, 10:20

... 14 15 16 Authors' contributions EW and LAM conceived of and designed the study MJ and KAT recruited subjects for and organized the focus groups EW analyzed and interpreted the data All authors ... from each woman including age, race, employment and marital status, and number of children, and the Women's Experience with Battering Scale (WEB[20]) and a modified version of the Conflict Tactics ... relationship and ranges in score from 10 to 60 where 20 or higher is indicative of battering The CTS measures frequency and severity of physical, emotional and sexual abuse in both current and former...

Ngày tải lên: 20/06/2014, 16:20

... humus The soils in the studied spruce stands are medium deep to shallow, loamy-sand and sandy-loam with a higher content of the skeleton in the lower layers and with a relatively low nutrient content ... is SSE and mean altitude 908 m a.s.l The age of trees was 28 years in 2005 The spruce stand is divided into two partial plots with different stand density (FD – dense stand, FS – sparse stand) ... Changes in soil, seepage water and needle chemistry between 1984 and 2004 after liming and N-saturated Norway spruce stand at the Höglwald, Germany Forest Ecology and Management, 233: 11–20 HÜTTL...

Ngày tải lên: 07/08/2014, 10:21

... outplanting 95%, 73% and 70%, respectively During the next four years it decreased to 78%, 61% and 50% and after overcoming the transplant shock the mean foliage increased again to 98%, 86% and 89%, respectively ... Hannerz M., Westin J., 2000 Growth cessation and autumn-frost hardiness in one-year-old Picea abies progenies from seed orchards and natural stands Scandinavian Journal of Forest Research, 15: ... Sunblad L.G., Strand M., Hällgren J.E., 2000b Phenotypic differences between natural and selected populations of Picea abies II Apical mitotic activity and growth related parameters Scandinavian Journal...

Ngày tải lên: 07/08/2014, 10:21

... 1987, 7, 141-142 Chandler, M L., Guilford, W G., Lawoko, C R., and Whittem, T Gastric emptying and intestinal transit times of radiopaque markers in cats fed a high-fiber diet with and without low-dose ... treatment and recovery Results of this study suggest that gastric electrical and contractile activities in conscious dogs were resistant to metoclopramide treatment During the postprandial state, ... pain and so on [24] Nam et al (1994) reported that electrical stimulation on BL-21 (Wei-Yu) greatly accelerated the ruminal contractile movement in cattle and had good effects on ruminal atony and...

Ngày tải lên: 07/08/2014, 14:23

... LB and TB The TB also correlated with RB and SB; LB with RB and FR, and AGB with RB and FR There were positive correlations for T and WT in the following cases: TB-LB, TB-AGB, TB-FR, LBAGB and ... eliminate soil and other rests The maximum length of the main root (RL) was measured and later AGB differentiating LB and SB, and RB differentiating thick roots (TR, diameter > mm) and fine roots ... leaves and fruit of glaucous-green colour in its early stages, and black-purple on maturation [5, 14] It is distributed throughout south and southeastern France, Italy (Alps), Corsica, Spain and...

Ngày tải lên: 08/08/2014, 00:22

... germination and seed deterioration but none of its interaction was significant (Tab I) Therefore, cold storage and GA treatment are indifferent to genotype DISCUSSION AND CONCLUSION 4.1 Cold storage and ... of argan stones set forth by Bani-Aameur and Alouani [3] was followed The fruits were hulled and the stones were scarified by slightly cracking them and then immediately soaked in 2% chlorine ... before seeding A randomised complete block factorial design was used, with 10 mother-trees, four cold storage scenarios and two GA treatments as factors [21] There were two blocks and the experimental...

Ngày tải lên: 08/08/2014, 01:22

... The best values for GP and MGT were always obtained in the range of 30-32 % (even 34 %) for the m.c., and X, X+2 and X+4 for the duration: GP varied between 74.5 and 79.5 % and MGT was around 12 ... receiving the dormancy breaking treatments, before and after drying and after 1, and years of storage After18 months of storage, the germination and nursery emergence of seeds that had been prechilled ... prechilled before storage (table III and IV) conclusion, the ability to dry and store non-dormant seeds has definitely opened new possibilities in the handling and preparation of dormant species...

Ngày tải lên: 08/08/2014, 14:21

... succeed in the stimulation of flowering in Norway spruce seedlings and cuttings in greenhouse and field conditions Using gibberellins (GA and girdling he ) 4/7 noticed a carry-over effect, observing ... Japanese and European larch grafts (Heitmüller and Melchior, 1960; Melchior, 1960; Bonnet-Masimbert, 1982), Douglas-fir seedlings (Pharis et al, 1980), Sitka spruce grafts (Philipson, 1985) and Engelmannwhite ... investigate the flowering response of Norway spruce seedlings to GA and ringing 4/7 applied alone and in combination MATERIALS AND METHODS spring of 1991 four full-sib families of Norway spruce...

Ngày tải lên: 08/08/2014, 18:21

... GrenobleI, France Moreland D.E & Novitsky W.P (1987) Effects of phenolic acids, coumarins and flavonoids on isolated chloroplasts and mitochondria In: Alletochemicals: Role in Agriculture and Forestry ... stage investigation of the chondria, like Moreland and Novitzky (1987), who showed that phenolic acids inhibited the electron transport chain, and by using various decoupling agents, such as ... as FCCP (Merlin, 1988), it should be possible to obtain a better understanding of the interactions between phytotoxins and respiratory mechanisms at the cellular level Merlin G (1988) Contamination...

Ngày tải lên: 09/08/2014, 04:20

... CL-19, and Renca cells were collected at 24, 48, and 72 hours They were analyzed by HPLC after deproteinization with methanol and derivatization with OPA for (A) L-arginine and (B) L-ornithine and ... collected data and wrote the manuscript; DJV participated in the study design and conducted western blots and arginase activity assays; YAC participate in the analysis of HPLC data and design and conducted ... micrograms of protein were tested for arginase I and arginase II expression by Western blot analysis Normal mouse liver and kidney were used as positive controls for arginase I and arginase II...

Ngày tải lên: 10/08/2014, 22:20

... variety They can randomly assign students to groups to maximize their heterogeneity: a mix of males and females, verbal and quiet students, the cynical and the optimistic (Fiechtner and Davis, 1992; ... the basis of random sampling and the data submitted to statistical analysis Besides, classroom is a special and restricted setting It is not possible to select so many teachers randomly to be ... Adams, Mc Leans and Castanos (1976) examine the quality and the quality of speech in both teacher – led class discussions and small group discussions The result is that the amount and variety of...

Ngày tải lên: 28/03/2015, 08:58

... standard parabolic subgroups of G, Pθ and Pϑ we say that (Pθ , Aθ ) dominates (Pϑ , Aϑ ) if and only if Pθ ⊃ Pϑ and Aθ ⊂ Aϑ Lemma 4.2.1.1 Consider two standard parabolic pairs (Pϑ , Aϑ ) and ... of a standard parabolic subgroup Pθ , W Mθ will denote the Weyl group of Mθ If we are given two standard parabolic subgroups P and P , we write their Langlands decomposition as P = Mθ N and P ... that θ = ϑ and that we have two orbits O and P containing discrete series representations σ and respectively Consider functions W[α,O,Pθ ];v and W[β,P,Pθ ];v1 ¯ ¯ where α = Λ(α ⊗ u) and β = Λ(β...

Ngày tải lên: 10/09/2015, 15:51