2d dige observations of het hepatic mitochondrial proteome 86

... levels by DIGE, silverstaining of DIGE gel, and immunoblotting 83 Figure 19 Immunoblotting of thioredoxin-2 and aconitase-2 85 Figure 20 2D- DIGE observations of HET hepatic mitochondrial ... disadvantages of major proteomic platforms 38 Table Experimental design of vehicle and drug-treatments of HET mice 50 Table Gel setup for 2D- DIGE experiments for HET hepatic mitochondrial proteome ... 56 Table Gel setup for 2D- DIGE experiments for analysis of troglitazone-induced changes in HET hepatic mitochondrial proteome 56 Table Hepatotoxicity score of HET mice treated with troglitazone...

Ngày tải lên: 12/09/2015, 11:08

... rate of plasma triglyceride input (intestinal and hepatic) and subtracted the rate of hepatic triglyceride secretion Briefly, to determine the total rate of triglyceride input in the plasma of mice, ... Rate of hepatic triglyceride secretion and intestinal triglyceride absorption in apoE) ⁄ ) and C57BL ⁄ mice One mechanism that could affect the hepatic triglyceride content is the secretion of hepatic ... (min) Fig Analysis of kinetic parameters associated with hepatic triglyceride content (A) Rate of hepatic very low-density lipoprotein (VLDL) triglyceride secretion (B) Rate of total triglyceride...

Ngày tải lên: 05/03/2014, 23:20

... effects of GH on the development of AFLD in the presence of alcohol are complex Both alcohol and GH exert a myriad of effects in vivo, and it is unclear whether the protective effects of GH against ... an important regulator of intrahepatic lipid metabolism Hepatic GH can interact with its receptor (GHR) on the surface of target cells and induces the association of GHR with Janus kinase (JAK)-2 ... percentage (VF%) of total body weight (VF weight/body weight × 100%) The hepatic index (HI) was calculated as liver weight/body weight × 100% Hepatic histology and measurement of triglyceride...

Ngày tải lên: 18/06/2014, 16:20

... and liver DNA of mice was observed The accumulation of titanium is consistent with the coefficients of liver and the liver injury of mice In addition, the accumulation of titanium of the organs ... decrease of ratio of ALB to GLB, TBIL levels which are important indicators of the hepatic injury, demonstrated that nano-anatase TiO2 induced hepatic injury Furthermore, the increase of enzyme activity ... histopathological changes of liver and the damage of hepatocyte substructure and with our previous report [12] Wang et al [8] showed that after a single oral gavage of dose of g/kg BW of TiO2 suspensions...

Ngày tải lên: 22/06/2014, 00:20

... of activated myofibroblasts) Secretion of type I collagen by myofibroblasts that leads to fibrosis was analyzed by immunofluorescence The results of imunofluorescence study for distribution of ... enhanced the process of differentiation Wang et al (2003) showed that hepatic differentiation of HSC could Page of 11 be enhanced by intravenous injection of soluble HGF None of the earlier reports ... distribution and stability of HGFCNP in the blood circulation Whether HGF released from the CNP causes the stem cell differentiation or apoptosis of myofibroflasts or both of these functions must...

Ngày tải lên: 11/08/2014, 00:23

... results indicate that mitochondrial translocation of Bax may constitute a direct cause of cytochrome c release ROS has also been demonstrated to induce the depolarization of the mitochondrial membrane, ... 500 lL of NaCl ⁄ Pi and 10 mL of 70% ethanol at °C overnight; then, after washing twice with NaCl ⁄ Pi, the cells were incubated with mL of PI staining solution (50 mgÆL)1 of PI and gÆL)1 of RNase ... NAC suppresses oridonin-induced mitochondrial membrane potential (MMP) collapse and apoptosis Subsequently, we examined the integrity of the mitochondrial membranes of cells by rhodamine 123 staining...

Ngày tải lên: 18/02/2014, 13:20

... quantities below the detection limit of XRD As a general rule, the detection limit of the XRD analyses of crystalline phases is considered to be 5% of the total weight of the mixture However, several ... where a large fraction of As (III) occurs as HAsO2y, the precip3 itation of CaHAsO3 will take place Within the same pH range, the formation of Ca3(AsO4)2 occurs in the presence of As(V) ions These ... include: degree of crystallinity, hydration, surface texture of the sample, sample weight, particle orientation, mass absorption coefficients of different minerals, etc (Mitchell, 1993) Any of these...

Ngày tải lên: 15/03/2014, 23:48

... Characterization of the purified soluble proteins The identity of the prepared protein was confirmed by sequence mapping of tryptic digests with MALDI-TOF MS with a good sequence coverage The total mass of the ... Instruments, Malvern, UK) Estimates of hydrodynamic radii of the expected molecular species were calculated with the software hydropro [26] The structures of monomers and dimers of the human CD69 in the ... asymmetric unit of the crystal structure of CD69 (the asymmetric unit comprises one dimer of CD69 and three Cl) anions) Every monomer binds two Cl– anions, one in a shallow pocket at the side of the molecule...

Ngày tải lên: 16/03/2014, 04:20

... serum levels of laminin (a component of intracellular matrix) were found to be predictive of any form of fibrosis in 30 patients with NAFLD.[36] The measurement of serum levels of other fibrogenic ... protective effects of estrogen In children the majority of patients are diagnosed during the second decade of life The exact prevalence of NAFLD in children is unknown The prevalence of fatty liver ... 247 World Journal of Pediatrics serum levels of aminoterminal propeptide of type III collagen, hyaluronic acid and tissue inhibitor of matrix metalloproteinase I in predicting hepatic fibrosis...

Ngày tải lên: 22/03/2014, 10:20

... initiation of the prescribed lifestyle modification, 49% of children were able to lose at least 10% of their baseline weight, and 86% of them showed significant improvement or normalisation of aminostransferases ... diagnosis of NAFLD required (1) confirmation of diffuse fatty infiltration of the liver in imaging studies regardless of aminotransferase levels;(2) average daily ethanol consumption of less than ... development of NAFLD.42 The main strengths of our study are the inclusion of children with the whole spectrum of NAFLD from simple steatosis to cirrhosis along with the long-term follow-up of up to...

Ngày tải lên: 22/03/2014, 10:20

... not treated well Signs of CLD AIH • AIH is a hepatic inflammation associated with the presence of circulating autoantibodies in the absence of other recognized causes of liver disease • Associated ... Presentation of cholestasis • Jaundice (accumulation of conjugated bilirubin) • • • • Pale stool (Acholic stool)?? Dark and foamy urine (bile salts in the urine) Pruritis (accumulation of bile salts) ... (accumulation of cholestrol) • Hepatomegaly +/- Splenomegaly • Failure to thrive (FTT)- neonates • Incidental lab finding Signs of cholestatic liver disease Hepato-cellular disease • Necrosis of hepatocytes...

Ngày tải lên: 28/03/2014, 09:20

... children developed acute hepatic failure with grade II-IV hepatic encephalopathy from weeks to months (median, month) after onset of symptoms In the remaining children the duration of disease before ... in a larger number of LKM1-positive patients, it suggests that the immunopathogenic mechanisms involved in the development of the forms of AIH may be different The severity of portal tract inflammation, ... than in LKM1-positive patients (38%) Of note is that 57% of patients, already cirrhotic at diagnosis, presented with a clinical picture reminiscent of that of prolonged acute viral-like hepatitis...

Ngày tải lên: 28/03/2014, 11:20

... [min] Fig 1: Inactivation kinetics of E coli as a result of plasma treatment of bacteria-containing sodium chloride (NaCl) solution ( ) as well as addition to E coli of plasmatreated NaCl solution ... of viable E coli suspended in sodium chloride solution without and with addition of different combinations of nitrate, nitrite, hydrogen peroxide and hydrochloric acid (HCl); exposure times of ... for different times The number of viable microorganisms (cfu ⋅ ml-1) was estimated by the surface spread plate count method using aliquots of serial dilutions of microorganism suspensions in...

Ngày tải lên: 18/05/2014, 20:35

... investigations involved determination of IMPDH activity, analyses of IMPDH and expression and characterization of T cell subpopulations The PK and PD profiles of MPA changed with time after transplantation ... range of total observations Profiles of patients in the belatacept group (n = 3) at weeks 1, and 13 (A, B and C) and the cyclosporine group (n = 3) at weeks 1, and 13 (D, E and F) Page 10 of 14 ... The postdose activities of IMPDH were strongly influenced by MPA exposure At week 1, the activity profiles for of the patients were inversely related to MPA concentraPage of 14 (page number not...

Ngày tải lên: 18/06/2014, 15:20

... without resection of hepatic lesions None of the patients had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the colon carcinoma ... by the Ethics Committee of our institution Patients were informed of the investigational nature of the study and provided their written informed consent Page of Follow-up of stage IIIB patients ... correlated with the status of hepatic metastasis: no hepatic metastasis (stage IIIB colon cancer without liver metastasis within years of follow-up), metachronous hepatic metastasis (stage IIIB...

Ngày tải lên: 18/06/2014, 16:20

... treatment of tumors associated with TSC, we used two models of TSC related tumors in a series of preclinical studies Tsc2 +/- mice were used to compare disease Page of 18 severity of kidney disease ... hydrolysis of L-asparagine to L-aspartic acid and is used as part of the curative combination chemotherapy regimen for the treatment of acute lymphoblastic leukemia (ALL) [36] The anti-tumor effect of ... months consisting of males and females, Tsc2+/+ months consisting of males and females, Tsc2+/- 12 months consisting of males and females, and Tsc2 +/+ 12 months consisting of males and females...

Ngày tải lên: 18/06/2014, 16:20

... four cycles of bortezomib, and hence an additional cost of USD211,200 Moreover, to see if early PR after one cycle of VAD may predict subsequent need of VTD salvage therapy, achievement of PR (i.e ... paraprotein) after one cycle of VAD was correlated with subsequent need of VTD salvage after three cycles of VAD by ChiSquare test Of the 23 patients with response data after one cycle of VAD, 11 (48.8%) ... i.e >50% reduction in paraprotein, after one cycle of VAD was correlated with subsequent need of VTD salvage upon completion of three cycles of VAD by Chi-Square test Methylation study Methylation-specific...

Ngày tải lên: 18/06/2014, 16:20

... Separated batches of × 106 cells of the freshly isolated mouse primary KCs were respectively transfected with μg of each of the four PV L1 plasmid $ 0HWK\OFHOOXORVH % 0HWK\OFHOOXORVH Cell morphology ... 0HWK\OFHOOXORVH % 0RG / / ,QYR 7XE 1DW / / ,QYR 7XE 0HWK\OFHOOXORVH Figure Effects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCs Effects of ... 7XE / 7XE 0HWK\OFHOOXORVH Figure Effects of pre-transfection treatment of methylcellulose on expression of the PV L1 genes in mouse primary KCs Effects of pre-transfection treatment of methylcellulose...

Ngày tải lên: 18/06/2014, 18:20

... symbol, and the total number of unique symbols formed the alphabet of the sequence Since the data was composed of a series of action potentials that form the response of the neurons to the input, ... dose of nicotine to study the behavior of DA neurons Therefore, these studies encouraged us to focus on the single, optimal dose to investigate the influence of nicotine on the dynamics of neural ... segment of data before the injection of nicotine was analyzed with LZ complexity method After firing rate of DA neuron has reached stable condition in response to nicotine, two minutes of data...

Ngày tải lên: 19/06/2014, 08:20

... instead of primary antibodies; pre-absorption with respective cognate peptides (150– 200 µg of peptide/ml of antibody working solution), omission of the secondary antibody and absence of immunoreactivity ... month of age Expression of BACE1 in neurons in APP [V717I] transgenic mice at month of age Representative confocal immunostaining of BACE1 positive cells and neuN positive neurons in the cortex of ... deposits of hyperphosphorylated Tau, causing formation of neurofibrillary tangles and finally neuronal death Aβ peptides are generated from amyloid precursor protein (APP) by sequential actions of...

Ngày tải lên: 19/06/2014, 22:20