analysis of transcriptional regulation in bone cells

... phhwu@sinica.edu.tw Institute of Physics, Academia Sinica, Nankang, Taipei 115, Taiwan Full list of author information is available at the end of the article nanoparticles influence the interaction ... importance of the 3D analysis which is made possible by our approach Background Quantitative analysis is an important but still largely unexplored issue in the study of nanomedicine procedures, in particular ... al Journal of Nanobiotechnology 2011, 9:14 http://www.jnanobiotechnology.com/content/9/1/14 RESEARCH Open Access Quantitative analysis of nanoparticle internalization in mammalian cells by high...

Ngày tải lên: 11/08/2014, 00:23

... sequence. Most of the E. coli TFs include a DNA-binding domain that determines target site specificity as well as a sensing domain that binds to small metabolites or to signaling proteins [2]. With ... test of whether neighbor regulation is associated with HGT. Evolutionary histories of TFs To clarify the origins of transcriptional regulation in E. coli, we conducted a detailed phylogenetic analysis ... Sequence of anfileHGTthe regulators of genomes sharedagainst of Click theyisillustratesand analysis of correlatesof of shared both by evolutionaryindividualsofpreference for HGTwe by Teichmann...

Ngày tải lên: 14/08/2014, 08:20

... Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, 100093 Beijing, People’s Republic of China Full list of author information is available at the end of the article ... to cytokinin and interacted with the auxin signaling pathway in root growth of the paper mulberry Ethylene regulates cell division in quiescent center and auxin biosynthesis in columella cells, ... directly or indirectly involve in the shoot development via the regulation of LBD in the paper mulberry It seemed that LBD TFs were the center link in the regulation of shoot development in the paper...

Ngày tải lên: 27/05/2020, 00:56

... role in the development of the current system of federal dredging laws IV STATE REGULATION OF DREDGING Notwithstanding federal dredging authorities, “state[] [governments] have an increasingly ... lightening, off-loading some of their goods, some of their oil in the middle of the bays, in the middle of the different ports outside of their berth, so that they can be lightened and come in and ... the mouth of the Penobscot River and linked to northern Maine and Montreal by rail, Searsport’s Mack Point Marine Intermodal Cargo Terminal (hereinafter “Mack Point”) is a significant international...

Ngày tải lên: 30/10/2022, 17:37

... the HIF-1a-driven increase in the survival of endothelial cells exposed to CyH is further increased by a NOS inhibitor but may be combated by (co-) administration of a PI3K ⁄ Akt inhibitor. The origins of cyclic ... during cyclic hypoxia; caution is therefore required in the use of NOS inhibitors as single anti-tumor treatments. More generally, by providing new insights into the regula- tion of HIF-1a in ... amplifi- cation of the HIF-1a response in endothelial cells and how the combination of both inhibitors could in? ??uence the fate of cells exposed to CyH. Figure 5A shows that the l-NAME-driven increased...

Ngày tải lên: 30/03/2014, 02:20

... containing only adenine and/or thymidine (WW dinucleotides), with antiphased periodic frequencies of dinucleotides containing cytidines and/or guanines (SS dinucleotides) [12] In mammalian genomes, ... arginine side-chain is located in the minor groove of all histone-DNA binding sites except for one, where the potential discriminator for direct read out is the adenine C2 group versus the guanine ... is shown on the y-axis Inset: average compositional profile of purine-purine and pyrimidine-pyrimidine dinucleotides between positions +40 and +200 The raw signal is shown in orange and a 3-bp...

Ngày tải lên: 09/08/2014, 20:22

... transcriptional profile of one LDOX gene (GSVIVT00001063001) showed increasing levels until 85 DAB, declining at 99 DAB, and increasing to the final stage in Norton berry skin, as observed in ... enzymes in the biosynthesis of flavonoids, lignin, anthocyanins and proanthocyanidins were expressed pre- ferentially in the berry skin of grapevine [9]. These genes include PAL, C4H,and4CL, encoding ... skin Page 19 of 23 Methods Collection of berry skin Berries from V vinifera... representation of qPCR and HPLC data for visualizing the coordination of transcriptional regulation...

Ngày tải lên: 11/08/2014, 11:21

... regulators, including Dof zinc-finger transcription factors, HD-zip transcription factor Athb-8, and MADS-box containing proteins, were included within this group of 58 genes Genes involved in stress/ ... number of downstream genes RING-finger protein The change in one of the predicted ciselements in the promoter of this gene was consistent with the changes in gene expression This finding is of particular ... Protein kinase, putative contains protein kinase domain, Pfam:PF00069 15069_s_at At2g28060 gb|AAC98460.1| AKINbeta3 protein, protein kinase-related At1g54610 gb|AAC64876.1| Similar to CRK1 protein...

Ngày tải lên: 14/08/2014, 14:21

... OSTEOCYTES PTH increases osteoclasts by upregulating RANKL in cells of the osteoblastic lineage, but the precise differentiation stage of the PTH target cell remains undefined Recent findings demonstrate ... lacking the distal control region regulated by PTH in the RANKL gene (DCR-/-) Longitudinal analysis of circulating Cterminal telopeptide (CTX) in male mice showed elevated resorption in growing ... Bellido in her big effort in planning and supporting this study iv ABSTRACT Abdullah Nasser Ben-awadh CONTRIBUTION OF RANKL REGULATION TO BONE RESORPTION INDUCED BY PTH RECEPTOR ACTIVATION IN OSTEOCYTES...

Ngày tải lên: 24/08/2014, 09:46

... M. and A. Ellinger (1985). "Localization of binding sites for concanavalin A, Ricinus communis I and Helix pomatia lectin in the Golgi apparatus of rat small intestinal absorptive cells. " J Histochem ... "The role of the membrane-spanning domain and stalk region of N-acetylglucosaminyltransferase I in retention, kin recognition and structural maintenance of the Golgi apparatus in HeLa cells. " J ... with golgin GCC185 [72], 12 those nucleated at the cis Golgi require GMAP210 and γ–tubulin-interacting proteins AKAP450 via GM130 binding... network of signaling proteins regulating GalNAc-T...

Ngày tải lên: 10/09/2015, 09:06

... presence of p53. An ontology analysis of these 54 proteins revealed that they were mainly involved in the regulation of cellular growth and proliferation. Downstream validation analysis using RT-PCR ... Pterin-4-alpha-carbinolamine dehydratase NADH dehydrogenase [ubiquinone] alpha subcomplex subunit Structural maintenance of chromosome NOL1/NOP2/Sun domain family protein Annexin A2 Isoform of ... PROTEOMIC ANALYSIS OF HYPOXIA INFLUENCE IN THE PRESENCE AND ABSENCE OF p53 IN HCT116 CELLS TAN WEE WEE (MSc.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL...

Ngày tải lên: 16/09/2015, 15:43

... recruitment of different chromatin binding proteins Hence making use of the split-ubiquitin system inside human cells for screening of interacting partners for human proteins, presents itself as a ... for cells growing in medium containing this selecting agent Posttranslational modifications of human proteins play an important role in governing their folding and hence determine the possible interacting ... step involving 6-TG and zeocin Isolation of genomic DNA and PCR amplification of the integrated DNA further eliminates any false positives obtained in 6-TG and zeocin 113 AAV-293 cells (Uninfected)...

Ngày tải lên: 29/09/2015, 13:01

... downregulated transcripts encoding U-box containing proteins likely contribute to thermotolerance in leaf cells of R stricta by maintaining proper protein folding and preventing protein degradation Upregulated, ... encoding aquaporins (AQPs) or major intrinsic proteins (MIPs) also occurred in leaves of R stricta (Additional file 12: Table S4) The main types of MIPs include plasma membrane intrinsic proteins ... WRKY in driving hsp genes, including the shsp20 Calmodulindependent protein kinases were induced in switchgrass by heat stress [100] A number of transcripts encoding calmodulin-dependent protein...

Ngày tải lên: 22/05/2020, 05:01

... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... for the regulation of egr-1 gene expression in the chromatin context The drastic down -regulation of Egr-1 mRNA levels, which is observed in n-3 treated cells, also indicates that other binding elements ... concentrations of the caveolin-1 and caveolin-3 proteins in the caveolae of cells stimulated with IL-1b in order to determine whether caveolin synthesis is also modulated by PUFA incorporation...

Ngày tải lên: 19/02/2014, 16:20

... prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose output in HepG2 cells Although TNFa-mediated inhibition of insulin ... decrease in Foxa2 occupancy of the PEPCK promoter This decrease was significantly (P < 0.01) attenuated in cells preincubated in the presence of TNFa prior to insulin incubation In cells incubated in ... to insulin, insulin signalling is prevented, resulting in abrogation of this inhibitory effect on PEPCK expression PEPCK overexpression, in turn, has been shown to attenuate insulin signalling...

Ngày tải lên: 16/03/2014, 02:20

... subsequent selection of single colonies onto plates resulted in loss of the integrated plasmid with an integration frequency of the interrupted nuoCD region in 50% of the integrants fdnG mutant ... containing the anaerobic respiratory chain (Figs and 2) Three other lines of evidence derived from in vivo and in vitro studies of nif regulation further supported our model: first, analysis of ... majority of the flavoprotein NifL in the mutant strains was not reduced at the cytoplasmic membrane resulting in high amounts of cytoplasmic NifL and thus in significant inhibition of NifA in the...

Ngày tải lên: 17/03/2014, 10:20

... findings [24] This indicated an increased extent of both bone formation and bone resorption with age, which together suggest an increase in the rate of bone turnover with ageing in control individuals ... Why does bone matrix contain non-collagenous proteins? The possible roles of osteocalcin, osteonectin, osteopontin and bone sialoprotein in bone mineralisation and resorption Cell Biol Int 1994, ... expression in OA and a decrease in controls [16] This finding is supportive of an increase or maintenance of bone volume in OA individuals versus the agedependent bone loss in the general population...

Ngày tải lên: 09/08/2014, 08:23

... of Analysis transcriptional regulation Analysis of transcriptional regulation Sliding window representation of absolute percentile difference between TR and RS variables (x-axis) against fold-change ... enrichment of genes belonging to protein complexes in the profiles with a predominance of TR over RS (Table 2) It is accepted that proteins belonging to the same complex must be present in similar ... Gerstein M: Analysis of mRNA expression and protein abundance data: an approach for the comparison of the enrichment of features in the cellular population of proteins and transcripts Bioinformatics...

Ngày tải lên: 14/08/2014, 08:20

... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... Analysis Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med ... order to minimize off-target effects and interpretation of results, perhaps these findings could contribute to a better interpretation of gene expression data, a better choice of suitable individual...

Ngày tải lên: 03/11/2012, 11:44

... considerable interest in “brown-like adipose cells in WAT These so called “brite” cells are present in WAT as evidenced by the presence of UCP1 expressing cells in WAT Studies in cell culture indicate ... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... deviation inflammatory response and IR development, were examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in eWAT Next,...

Ngày tải lên: 06/03/2014, 19:20