Molecular characterization of the zebrafish ff1b gene

... promoter 176 Generation of the ff1bEx2EGFP transgenic zebrafish: a major step for the lineage tracing of ff1b- expressing cells 177 6.2.1 The EGFP transgene expression parallels the endogenous ... pattern of ff1b in the VMH 178 6.2.2 The EGFP transgene expression parallels the endogenous expression pattern of ff1b in the interrenal gland 180 6.2.3 The EGFP fluo...

Ngày tải lên: 14/09/2015, 14:10

... pattern with other species 83 4.5.1 Zebrafish twist1 a and twist1 b genes 83 4.5.2 Zebrafish twist2 85 4.5.3 Zebrafish twist3 86 4.6 Shared and unique expression sites of the zebrafish twist genes 86 ... proteins generated by the neighbor-joining method Figure 3.6: Gene structure of twist1 a, twist1 b, twist2 and twist3 Figure 3.7: RT-PCR of zebrafish...

Ngày tải lên: 16/10/2015, 15:38

... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Purification of beta...

Ngày tải lên: 23/03/2014, 13:20

... TERQANFLGK R .K IWPSHKGRPG Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y R NFLQSRPE ... pathogenesis of the virus In this study, we have characterized and analyzed the genetic diversity and population dynamics of the gag NC and p6 genes from six mother-infant pairs following vertical ... therapy Symptomatic AIDS Symptomatic AIDS Asymptomatic Asympto...

Ngày tải lên: 13/08/2014, 09:21

... very close to that of the mouse Muc4 (Table 1) The size of intron of the human MUC4 has been estimated to be  15 kb by restriction mapping [12] and to be at least 20 kb by our analysis of the human ... small mucin [26] The consensus sequence of the Muc4 tandem repeat is very close to that of SMC Nevertheless, the tandem repeat domain of Muc4...

Ngày tải lên: 08/03/2014, 23:20

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of...

Ngày tải lên: 30/03/2014, 13:20

... that of < /b> the < /b> positive control cells was observed indicating an interaction between the < /b> PmrD and PmrA Analysis of < /b> EMSA indicates a direct binding of < /b> the < /b> recombinant PhoP to pmrD The < /b> binding of < /b> PhoP ... presence of < /b> the < /b> connectormediated pathway < /b> in K pneumoniae In summary, involvement of < /b> Klebsiella pmr in...

Ngày tải lên: 10/08/2014, 05:21

... Novirhabdovirus genus The termini of the viral genome have conserved sequences at the 3'-end (CAUAG/UU) and 5'-end (G/AAUAUG) as other members of the Novirhabdovirus genus The first nt of the leader ... comparing the deduced amino acid sequences of known rhabdoviruses reveals that viruses belonging to the same genera of Vesiculovirus, Lyssavirus, Ephemerovirus, Nov...

Ngày tải lên: 12/08/2014, 04:20

... members of the Novirhabdovirus genus The termini of the viral genome have conserved sequences at the 3’-end (CAUAU) and at the 5’-end (GUAUA) as other members of Novirhabdovirus genus Out of first ... start The stop codon of NV ORF is merged with gene end sequence and is shown in red box B) Complementarities of the 3’- and 5’-ends of the IHNV genome The first 1...

Ngày tải lên: 12/08/2014, 04:21

... impact on MOST- 1 function 11 9 MOST- 1 Protein 12 1 Aggregation and implication of MOST- 1 function 12 3 Interactors and their possible function with MOST- 1 126 MOST- 1 Expression and Cell Cycle 13 2 Current ... synchronization studies 94 v 4 .11 Yeast two hybrid screening 10 2 4 .12 Overexpression and RNA interference studies 11 0 CHAPTER 5: DISCUSSION Strate...

Ngày tải lên: 16/09/2015, 17:13

... the mechanism of transcriptional regulation of the FSH subunit gene promoter of the Chinook salmon The Chinook salmon FSH subunit gene and 5’ flanking sequence will be isolated, sequenced and ... regard to the size of introns and 3’ untranslated region (3’ UTR; Figure 3) Compared to the 0.3 – 0.35 kb size of intron of the LHβ genes, the size...

Ngày tải lên: 08/11/2015, 17:15

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the heli...

Ngày tải lên: 18/02/2014, 18:20

... Mapping of transcription initiation sites in the human DNASE1 gene To identify the transcription initiation sites of DNASE1 in pancreas, 5¢-RACE based on RNA ligasemediated and oligo-capping ... including characterization of the promoter region of the gene and the associated transcriptional factors Therefore, delineation of the transcriptional Promot...

Ngày tải lên: 19/02/2014, 06:20

... stearothermophilus adenylate kinase with bound Ap5A, Mg2+ Ap5A, and Mn2+ Ap5A reveal an intermediate lid position and six coordinate octahedral geometry for bound Mg2+ and Mn2+ Proteins 32, 27 6 28 8 29 Kanaani, ... M (1996) Ancient divergence of long and short isoforms of adenylate kinase: molecular evolution of the nucleoside monophosphate kinase family FEBS Lett...

Ngày tải lên: 21/02/2014, 00:20

... within )260/)119, we Ó FEBS 2002 Integrin a3 gene promoter (Eur J Biochem 269) 4529 Fig Effects of mutations in the Ets- and GATA-binding sites and the E-box of the mouse a3 integrin gene on promoter ... assay (EMSA) using the Ets consensus site at )133 As the involvement of the Ets consensus binding site at )133 in the promoter activity of th...

Ngày tải lên: 21/02/2014, 03:20