molecular aspects of the insulin like growth factor igf genes

... Miller, R.E & Cascieri, M.A (1990) The roles of tyrosines 24, 31, and 60 in the high anity binding of insulin- like growth factor- I to the type insulin- like growth factor receptor J Biol Chem 265, ... analysis of IGF- I and IGF- I analogue binding to highanity recombinant IGF1 R Binding of IGF- I (A), des-(1±3) -IGF- I (B), Arg3 -IGF- I (C), Gly3 -IGF- I (D), Long IGF- I (E), Ala31 -IGF- I (F), Leu24 -IGF- I ... in the af®nities of IGF- I (4.45 nM) and IGF- II (17.8 nM) for the rhIGF1R (Figs 1A and 2A, Table 1) Substitution of Glu at position to Arg (Arg6 -IGF- II) or deletion of the ®rst six amino acids of...

Ngày tải lên: 08/03/2014, 22:20

... Insulin- like Growth Factor; (IGF1 R): Insulin- like Growth Factor- 1 receptor; (IGFBP-3): Insulin- like Growth Factor Binding Protein-3; (IGFBP-4): Insulin- like Growth Factor Binding; Protein-4; (MAPK): ... role of secreting IGFBPs in melanoma However, data not support the clinical utility of measuring levels of IGFBP-3 and -4 in sera of melanoma patients Abbreviations (IGF) : Insulin- like Growth Factor; ... The insulin- like growth factor (IGF) axis is one such system which contributes to human malignancy, with overexpression of IGF1 receptor (IGF1 R) noted in several cancers, including melanoma The...

Ngày tải lên: 18/06/2014, 15:20

... diameter; IGF- I, Insulin- like growth factor- I; IGF- I zSDS, SDS of IGF- I levels according to age; IGFBP-1, IGF- binding protein-1; IGFBP-3, IGF- binding protein-3; IGF- I/ IGFBP-3, IGF- I/IGFBP-3 ratio ... diameter; IGF- I, Insulin- like growth factor- I; IGF- I zSDS, SDS of IGF- I levels according to age; IGFBP-1, IGF- binding protein-1; IGFBP-3, IGFbinding protein-3; IGF- I/IGFBP-3, IGF- I/IGFBP-3 ratio ... imaging, or by measurement of other cytokines (mainly IL-6) to further support the hypothesis that the impairment of the IGF- I axis in obesity might represent different aspects of the chronic inflammation...

Ngày tải lên: 18/06/2014, 22:20

... diameter; IGF- I, Insulin- like growth factor- I; IGF- I zSDS, SDS of IGF- I levels according to age; IGFBP-1, IGF- binding protein-1; IGFBP-3, IGF- binding protein-3; IGF- I/ IGFBP-3, IGF- I/IGFBP-3 ratio ... diameter; IGF- I, Insulin- like growth factor- I; IGF- I zSDS, SDS of IGF- I levels according to age; IGFBP-1, IGF- binding protein-1; IGFBP-3, IGFbinding protein-3; IGF- I/IGFBP-3, IGF- I/IGFBP-3 ratio ... imaging, or by measurement of other cytokines (mainly IL-6) to further support the hypothesis that the impairment of the IGF- I axis in obesity might represent different aspects of the chronic inflammation...

Ngày tải lên: 20/06/2014, 04:20

... Silkmoth IGF -like peptide N Okamoto et al Members of the insulin- like peptide (ILP) family are present in a wide variety of metazoans In vertebrates, insulin and insulin- like growth factors (IGFs) ... Efstratiadis A (1993) Role of insulin- like growth factors in embryonic and postnatal growth Cell 75, 73–82 26 Holt RIG (2002) Fetal programming of the growth hormone insulin- like growth factor axis Trends ... days of cultivation, the number of the disk cells was approximately 30% larger than in the control (Fig 6M) Considering the BrdU incorporation in specific areas of the disks (Fig 6E), the rate of...

Ngày tải lên: 18/02/2014, 13:20

... representation of the susceptibility to proteolytic degradation of IGFBP-1 isoforms The y-axis shows the percentage of the ratio between the spot area of the degraded and that of the untreated protein ... elevation of insulin- like growth factor (IGF) binding protein-1 decreases plasma free IGF- I and muscle protein synthesis Endocrinology 144, 3922–3933 Westwood M (1999) Role of insulin- like growth factor ... suggest that not only the degree, but also the position of the phosphate groups influence the process As for the other IGFBPs, the proteolytic cleavage of IGFBP-1 occurs within the central domain...

Ngày tải lên: 07/03/2014, 00:20

... Glycemic derangements related to insulin- like growth factors such as the pro -IGF- IIE and insulin- like growth factor- binding proteins have been described in GISTs, and they may become more important ... Hankinson SE: Insulin- like growth factors and neoplasia Nat Rev Cancer 2004, 4:505-18 20 21 Tao Y, Pinzi V, Bourhis J, Deutsch E: Mechanisms of diseases: signalling of the insulin- like Growth Factor ... functional studies demonstrate the pathogenetic role of IGF1 R in WT GISTs, the IGF axis blockade may be beneficial in the treatment of GIST However, in-depth analysis of the IGF axis in GISTs is mandatory,...

Ngày tải lên: 18/06/2014, 16:20

... between the induction of SOCS and both the presence of sepsis and the administration of PN [14] Administration of 16 hours of PN was associated with induction of the expression of hepatic mRNA of the ... Circulating levels of IGF- I and of the insulin- like growth factor binding protein-I (IGFBP-I) are exquisitely sensitive to provision of nutrients, the former being increased and the latter being ... of mRNA of both IGF- I and the GHR, while these were not affected by sepsis These results support a potential effect of nutrition and the route of administration of nutrition on the activity of...

Ngày tải lên: 13/08/2014, 08:20

... binding of IGFs to IGFBPs limits the interaction of the growth factors with the cell surface receptors Therefore, IGFBPs generally inhibit IGF- stimulated cell growth in vitro [25] In contrast, IGFBP-rP1 ... fluids, IGFBPs bind IGFs with high affinity, protecting the growth factors from proteolytic degradation The proteolytic cleavage of IGFBPs is thought to contribute to the release of IGFs from IGF ⁄ IGFBP ... (1997) Identification of a family of low-affinity insulin- like growth factor binding proteins (IGFBPs): characterization of connective tissue growth factor as a member of the IGFBP superfamily Proc...

Ngày tải lên: 19/02/2014, 07:20

... skeletal muscle is the insulin like growth factor- 1 (IGF- 1) ⁄ insulin- dependent pathway [5,6] Stimulation of muscle cells with growth factors such as IGF- 1 leads to the activation of phosphoinositide ... effects of U0126 treatment on IGF1 -induced phosphorylation of either Akt or S6K1 (Fig S1C) These results support the hypothesis that IGF- 1-induced activation of mTOR signaling is mediated by the ... phosphorylation of TSC2 by Akt caused the translocation of TSC2 protein from the membrane to the cytosolic fraction On the basis of these findings, we examined whether or not IGF- 1 stimulation...

Ngày tải lên: 15/03/2014, 11:20

... show whether all the effects are mediated by the IGF- 1R With this in mind we determined whether the naturalistic product of IGF- I cleavage within the brain that acts independent of the IGF- 1R ... part of the central action of IGF- I Mature intact IGF- I has antidepressant activity, but it is still not known if des-(13) -IGF- I, acting via the IGF- 1R, or the generation of GPE from exogenous IGF- I ... found that IGF- I induced the expression of several BDNF transcripts [11]; another possible mediator of its anti-depressant activity One of the more interesting aspects of BDNF biology is the differential...

Ngày tải lên: 19/06/2014, 22:20

... establish the similarities and differences between the mammalian IGF- IGFBP system and the Drosophila Dilp-Imp-L2 system Characterization of the Dilps at the protein level, and of whether and how they ... 18.2 Journal of Biology 2008, Volume 7, Article 18 Alic and Partridge binding proteins (IGFBPs) [8] The effects of IGFBPs on IIS are complex IGFBPs act as regulators of the activity of IGFs, by prolonging ... IIS is an important regulator of growth, and overexpression of the Drosophila insulin receptor in the eye during development results in hyperplasia (overgrowth) of the eye Honegger and coworkers...

Ngày tải lên: 06/08/2014, 18:21

... replacement therapy has been shown to increase bone mass [3] GH is the primary inducer of IGF- 1 synthesis by the liver and a central regulator of the concentration of circulating IGF- 1 [3] IGF- 1 has ... interestingly, the change of ESR during the course of the study was highly correlated with the change of IL-6 and to a lesser degree was inversely correlated with the change of IGF- 1 However, ... increased Both of these results — the effects on the IL-6/ sIL-6R pathway and on IGF- 1 in the endocrine system — may be involved in the mechanisms mediating the beneficial effects of HRT There is a...

Ngày tải lên: 09/08/2014, 01:21

... cultured factor (IGF) -1 to IGF- binding proteins (IGFBPs), in insulin- like of NBI-31772, a small-molecule inhibitor of the binding of priActivitygrowth rabbit chondrocytes lin -like growth factor (IGF) -1 ... binding R insulin- like analog of IGF- 1 with to IGF- binding proteins for IGFBPs) primary cultured humanNBI-31772, a small-moleculeincubatedof the IGF- 1 orof IGF- 1 (an growth factor (IGF) -1 a greatly ... dependent on the level of secreted IGFBPs, and the addition of IGFBP-3 blocked the effects of IGF- 1 [31] According to the growing evidence that IGFBPs (and especially IGFBP-3) are involved in the decline...

Ngày tải lên: 09/08/2014, 01:23

... dissociate the IGF- 1-IGFBP complex Then the serum sample is returned to physiological pH in the presence of excess IGF- 2 provided in the kit The excess IGF- 2 saturates the refolded IGFBPs present in the ... VA, Davidson B: Diagnostic and prognostic role of the insulin growth factor pathway members insulin- like growth factor- II and insulin- like growth factor binding protein-3 in serous effusions Hum ... enlarged view of a cell indicating the contact surfaces of the source and the drain is shown to the right of the wafer Gate voltage is applied at the back of the wafer Also indicated are the aligning...

Ngày tải lên: 11/08/2014, 00:23

... contributions SS designed the constructs and contributed to the conduct of the experiments, analysis of the data, and preparation of the manuscript SM and RD contributed to the conduct of the experiments ... transduction and the cells were washed with growth medium, then ml of fresh growth medium was added to each well, and the cells were further cultured for two days IGF- I analysis IGF- I in the conditioned ... a plasmid The second is the AAV helper function of Rep and Cap proteins The third is the adenoviral helper function provided by the products of the adenovirus E2A, E4 and VA genes There are two...

Ngày tải lên: 12/08/2014, 04:21

... elongatus using plasma insulin- like growth factor (IGF1 ) with the ultimate goal of measuring spatial differences in relative growth rates Insulin- like growth factor is one of the principal hormones ... have turned to the endocrine system to develop new nonlethal approaches to measure growth One of the principal hormones regulating growth is insulin- like growth factor (IGF1 ) In the laboratory, ... review of the current literature on IGF1 and growth in fish, that IGF1 could provide a valid index of growth in fish However, there are no data to suggest that IGF1 can provide an absolute measure of...

Ngày tải lên: 04/09/2015, 17:15

... (1996) Growth hormone and the insulin- like growth factor system in myogenesis Endocrine Rev 17, 481–517 DeVol DL, Rotwein P, Sadow JL, Novakofski J & Bechtel PJ (1990) Activation of insulin- like growth ... (1993) A Role of insulin- like growth factors in embryonic and postnatal growth Cell 75, 73–82 Louvi A, Accili D & Efstratiadis A (1997) A growthpromoting interaction of IGF- II with the insulin receptor ... insulin- like growth factor binding protein-5 cDNA, which renders the cells unresponsive to endogenous IGF- 1 These cells undergo minimal differentiation without the inclusion of exogenous R3 -IGF- 1,...

Ngày tải lên: 19/02/2014, 06:20

... USA) DNA quanti®cation The DNA content of the cell layer at the end of incubation was quanti®ed by the method of West et al [31] After solubilization of the cell layer in M NaOH and subsequent ... implication of cAMP increase, the second messenger of FSH, in the bFGF regulation was evaluated by addition to the culture medium of either mM dbcAMP or 10 lgámL)1 cholera toxin Their addition ... testicular development, the physiology of Sertoli cells is modi®ed The cell proliferation decreases and ceases allowing the establishment of the hematotesticular barrier around the 20th day postpartum...

Ngày tải lên: 21/02/2014, 03:20

... in the presence of the GSH ⁄ oxidized GSH (GSSG) redox couple The RP-HPLC profile of the purified, reduced J1ex6 peptide is also shown mutant, the correct folding and the complete formation of the ... obtained in the present study suggest the opposite There are several lines of evidence in support of this hypothesis: the dramatic impairment of the oxidative folding in vitro induced by the G274D ... Altered flexibility in the rod -like structure of the DSL ⁄ EGF1-3 structure [26] might also affect the docking of the ligand to the receptor The oxidative folding in vitro of larger constructs...

Ngày tải lên: 16/03/2014, 00:20