... studied the differential gene expression in the liver of P annectens at the end of the induction phase and the beginning of the maintenance phase Their results confirmed the importance of reconstruction ... from the primitive pharynx The study of the respiratory system of the lungfish is not only of special interest in our understanding the functional and structural design of the lungs in the early ... aestivation The lung is one of the most important organs in the lungfishes necessary for survival However, to date, there is a dearth of knowledge on the molecular biology of the lung of any lungfish...
Ngày tải lên: 09/09/2015, 17:55
Genetics and Molecular Biology
... called the minor and major grooves of the DNA Thus, the displacement of the base pair from the helix axis primarily affects the relative depth of the two grooves, and the twisted position of the ... Cell Structure and Function the surface of the chromosomal mass The number of times, N, that the DNA must wind back and forth will then be related to the length of the DNA and the volume in which ... synthesis of the amino acid and that there is no leakage of the amino acid out of the cell For about the first 15 sec, the incorporation of radioactive amino acid into protein increases as t2 and...
Ngày tải lên: 13/03/2014, 21:55
... Although the precise localization of the domains responsible for receptor binding and translocation activity remains unclear, these domains are located in the N-terminus of each of these toxins and ... is the detection of the modification, which involves the detection of a molecular mass change of Da in a relatively large protein substrate (20–45 kDa) Another challenge is the development of ... current understanding of the structure–function, mechanism of action and cellular consequences of this newest member of the G-protein-deamidating dermonecrotic toxin family Epizootic and zoonotic...
Ngày tải lên: 22/03/2014, 15:21
Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the Ó FEBS 2004 ... b-sandwich are composed of seven b-strands Many of the side-chains in the b-sheets are hydrophobic, and interactions between these residues appear to hold the b-sandwich in position With the ... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant...
Ngày tải lên: 23/03/2014, 13:20
molecular biology of the parathyroid - tally naveh-many
... MOLECULAR BIOLOGY INTELLIGENCE UNIT NAVEH-MANY MBIU Tally Naveh-Many Molecular Biology of the Parathyroid Molecular Biology of the Parathyroid MOLECULAR BIOLOGY INTELLIGENCE UNIT Molecular Biology ... description of the major aspects of the molecular biology of the parathyroid gland, PTH production and secretion The book is designed for students and teachers as well as scientists and investigators ... Finally, the last chapter focuses on the characteristics of PTH-null mice and the skeletal and reproductive abnormalities that they present Together the chapters of this book offer a state of the...
Ngày tải lên: 08/04/2014, 12:52
Báo cáo y học: "Disruption and pseudoautosomal localization of the major" ppt
... are the swine leukocyte antigen (SLA) complex, where the centromere of chromosome separates the class II region from the other parts of the MHC [34] and the recently described class I genes of the ... genes They found eight 200-900 kbp scaffolds that contained orthologs to many of the genes in the eutherian MHC The interpretation is that the syntenies of the MHC region are ancient and predate the ... the echidna sex chromosome chain and the positions of the MHC chromosomes contained therein The comparative mapping of the MHC in echidna and platypus provides evidence for homology between the...
Ngày tải lên: 14/08/2014, 08:20
EVOLUTION OF THE MOLECULAR BIOLOGY OF BRAIN TUMORS AND THE THERAPEUTIC IMPLICATIONS pdf
... Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications high grade gliomas The structure and compounds of the ECM of the brain tissue have many specific differences from other ... infiltrate the enviro‐ ment and form it similar to the tumor tissue The process of the peritumoral invasion de‐ pends on the confrontation zone of the tumor cells and the non-neoplastic cells and ECM ... Overexpression of dystroglycan decreased Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications the growth rate of glioma cell lines so it was found to be involved in the progression...
Ngày tải lên: 23/03/2014, 10:20
molecular and cellular biology of neuroprotection in the cns - christian alzheimer
... Molecular and Cellular Biology of Neuroprotection in the CNS Molecular and Cellular Biology of Neuroprotection in the CNS Edited by Christian Alzheimer Institute of Physiology University of ... that determine the fate of CNS neurons in the face of acute or chronic insults The book is written mostly from the viewpoint of the basic scientist who works at the cellular and molecular level, ... Brief Overview of Their Molecular Biology, Biochemistry β and Signaling 354 Expression of TGF-βs and TβRs in the Nervous System 358 β β TGF-βs and the Regulation of Proliferation,...
Ngày tải lên: 08/04/2014, 12:51
Tài liệu OXFORD DICTIONARY OF Biochemistry and Molecular Biology REVISED EDITION Managing Editor Professor pdf
... in the text are those of the 18column format of the table given in the 1990 edition of the IUPAC ‘Red Book’ The correspondence between this and other versions of the table is described in the ... two-stranded helical polymer of the protein actin Actin filaments form the thin filaments of muscle and also the microfilaments of the cytoskeleton of eukaryotic cells Hence they are a major component ... or produced by the adrenal glands the adrenal gland itself adrenal androgen any of the C19 steroid hormones produced in the cortex of the adrenal gland, including androstenedione and testosterone...
Ngày tải lên: 23/01/2014, 07:20
Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation of the overall structure of the WW and WWW mutants – indicate that these...
Ngày tải lên: 21/02/2014, 15:20
MORPHOGENESIS THE CELLULAR AND MOLECULAR PROCESSES OF DEVELOPMENTAL ANATOMY pptx
... biologists and wish became the father of thought and the grandfather of observation: they claimed to see small but fully formed organisms in the sperm of men, horses, cocks and other animals and also ... distinct tissues come together, and here we will examine the generation of the neural tube in the newt and the formation of the ducted submandibular glands of the mouse The latter example in particular ... development, the position of epithelial folding, the direction of spindle orientation and hence the direction of cell growth, the amount of sac contraction, and the symmetry of epithelial drying...
Ngày tải lên: 05/03/2014, 11:21
Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... properties of both conserved and unique components of the Cag-T4SS, together with potential implications for the current understanding of its mode of action The cagPAI and the Cag type IV secretion ... details of secretion apparatus assembly, as well as in the molecular mechanisms of effector protein secretion This minireview describes the composition of the cagPAI and the properties of both ... complex proteins A further step in the assembly of the secretion apparatus would be the recruitment of components forming the inner membrane pore of the secretion apparatus The Cag system contains...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... and sequenced as above The fulllength cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed from the sequence of the extreme 5¢ end of lac1 The ... abundance was evaluated in the exponential phase of the reaction (half of the maximum product) To further validate the assays, the optimized cycle number (23 and 28 for gpd and lac1, respectively) ... purified and characterized one of these laccases, lac1, and cloned and sequenced the cDNA encoding the protein RT-PCR was used to study the regulation of lac1 gene expression in V volvacea when the...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Functional fine-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf
... induced against the linear isoform of the HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature of the sera, the importance of residues ... with the intact protein (Fig 8B,F) For the binding of the sera to the HNE peptide, the importance of the Cys residues was relatively low, in comparison to the binding with the mAbs BH216, BH21 and ... Similarly, the e-amino group of K387 could form a salt bridge with the close carboxyl group of E395 and further stabilize the peptide structure within the complex with the antibody In the virus, the...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1 pdf
... expulsion of respirable micron size allergen-containing particles from grass pollens [10,11] The small size of these subcellular particles allows them to reach the deeper airways and may explain the ... Comparison of natural and recombinant group grass pollen allergens Although the majority of rPhl p was detected in the insoluble pellet fraction of infected insect cells, up to 0.75 mgÆL)1 of soluble ... with the corresponding preimmune serum (Fig 1C) A band of approximately double the molecular mass as the purified allergens, possibly representing a dimer, was detected in the bacterial and insect...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B gene, the human...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo khoa học: Purification, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx
... disulfide bond These estimates of the molecular mass of ST94 were supported by the result of gel permeation HPLC (Fig 3) There was a minor band between 140 and 232 kDa in the native-PAGE of Fig 2A ... with trypsin The molecular masses of the subunits of ST94 and ST94t were similar to those of the proenzymes ( 70–80 kDa) and the activated enzymes by proteolysis ( 60–70 kDa) of a bivalve tyrosinase ... affect the activities of ST94 and ST94t Table Effects of tyrosinase inhibitors on the o-diphenolase activities of ST94 and ST94t The o-diphenolase activities of ST94 and ST94t were measured by the...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... i.e DIMs and PGL-tb; and (b) to address the question of the function of the two related molecules DIM A and DIM B According to the accepted model of DIM and PGL biosynthesis [17], DIM A and PGL-tb ... bacteria of the PMM56 mutant strain were present in the spleen of these mice For the determination of the number of PMM56 bacteria present in the spleen of these four mice, we thus chose a value of ... roles of the various members of the DIM family, e.g DIM A and DIM B, in these functions have never been investigated The recent developments in our understanding of the biosynthetic pathway of DIMs...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the ... of WT and the other of rWT metHb, exhibit differences that include important portions of the protein that we have characterized above Thus parts of the FG corner move away from the heme and the...
Ngày tải lên: 23/03/2014, 17:22