... studied the differential gene expression in the liver of P annectens at the end ofthe induction phase andthe beginning ofthe maintenance phase Their results confirmed the importance of reconstruction ... from the primitive pharynx The study ofthe respiratory system ofthe lungfish is not only of special interest in our understanding the functional and structural design ofthe lungs in the early ... aestivation The lung is one ofthe most important organs in the lungfishes necessary for survival However, to date, there is a dearth of knowledge on themolecularbiologyofthe lung of any lungfish...
... called the minor andmajor grooves ofthe DNA Thus, the displacement ofthe base pair from the helix axis primarily affects the relative depth ofthe two grooves, andthe twisted position ofthe ... Cell Structure and Function the surface ofthe chromosomal mass The number of times, N, that the DNA must wind back and forth will then be related to the length ofthe DNA andthe volume in which ... synthesis ofthe amino acid and that there is no leakage ofthe amino acid out ofthe cell For about the first 15 sec, the incorporation of radioactive amino acid into protein increases as t2 and...
... Although the precise localization ofthe domains responsible for receptor binding and translocation activity remains unclear, these domains are located in the N-terminus of each of these toxins and ... is the detection ofthe modification, which involves the detection of a molecular mass change of Da in a relatively large protein substrate (20–45 kDa) Another challenge is the development of ... current understanding ofthe structure–function, mechanism of action and cellular consequences of this newest member ofthe G-protein-deamidating dermonecrotic toxin family Epizootic and zoonotic...
... With the exception of four a-helices and two pairs of short b-strands, the rest ofthe protein consists almost entirely of loops connecting the b-strands The loops extending from the Ó FEBS 2004 ... b-sandwich are composed of seven b-strands Many ofthe side-chains in the b-sheets are hydrophobic, and interactions between these residues appear to hold the b-sandwich in position With the ... was 1.8°, the standard deviation ofthe hydrogen bond energies was 0.7 and overall G-factor, a measure ofthe normality ofthe structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant...
... MOLECULARBIOLOGY INTELLIGENCE UNIT NAVEH-MANY MBIU Tally Naveh-Many MolecularBiologyofthe Parathyroid MolecularBiologyofthe Parathyroid MOLECULARBIOLOGY INTELLIGENCE UNIT MolecularBiology ... description ofthemajor aspects ofthemolecularbiologyofthe parathyroid gland, PTH production and secretion The book is designed for students and teachers as well as scientists and investigators ... Finally, the last chapter focuses on the characteristics of PTH-null mice andthe skeletal and reproductive abnormalities that they present Together the chapters of this book offer a state of the...
... are the swine leukocyte antigen (SLA) complex, where the centromere of chromosome separates the class II region from the other parts ofthe MHC [34] andthe recently described class I genes ofthe ... genes They found eight 200-900 kbp scaffolds that contained orthologs to many ofthe genes in the eutherian MHC The interpretation is that the syntenies ofthe MHC region are ancient and predate the ... the echidna sex chromosome chain andthe positions ofthe MHC chromosomes contained therein The comparative mapping ofthe MHC in echidna and platypus provides evidence for homology between the...
... Evolution oftheMolecularBiologyof Brain Tumors andthe Therapeutic Implications high grade gliomas The structure and compounds ofthe ECM ofthe brain tissue have many specific differences from other ... infiltrate the enviro‐ ment and form it similar to the tumor tissue The process ofthe peritumoral invasion de‐ pends on the confrontation zone ofthe tumor cells andthe non-neoplastic cells and ECM ... Overexpression of dystroglycan decreased Evolution oftheMolecularBiologyof Brain Tumors andthe Therapeutic Implications the growth rate of glioma cell lines so it was found to be involved in the progression...
... Molecularand Cellular Biologyof Neuroprotection in the CNS Molecularand Cellular Biologyof Neuroprotection in the CNS Edited by Christian Alzheimer Institute of Physiology University of ... that determine the fate of CNS neurons in the face of acute or chronic insults The book is written mostly from the viewpoint ofthe basic scientist who works at the cellular andmolecular level, ... Brief Overview of Their Molecular Biology, Biochemistry β and Signaling 354 Expression of TGF-βs and TβRs in the Nervous System 358 β β TGF-βs andthe Regulation of Proliferation,...
... in the text are those ofthe 18column format ofthe table given in the 1990 edition ofthe IUPAC ‘Red Book’ The correspondence between this and other versions ofthe table is described in the ... two-stranded helical polymer ofthe protein actin Actin filaments form the thin filaments of muscle and also the microfilaments ofthe cytoskeleton of eukaryotic cells Hence they are a major component ... or produced by the adrenal glands the adrenal gland itself adrenal androgen any ofthe C19 steroid hormones produced in the cortex ofthe adrenal gland, including androstenedione and testosterone...
... a4-helix to the b4 strand andthe b9 strand to the b10 strand at the bottom ofthe Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis ofthe complete sequence ofthe cryptic plasmid pIT3 isolated from the...
... illustrates the overall saddle shape ofthe dimeric structure andthe key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top ofthe hollow (Fig 1A) The ... the C-terminal domain of apoCII interacts with the interface ofthe N- and C-terminal domains of LPL and part ofthe lid surface [32] Because the functional importance of LPL’s C-terminal domain ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation ofthe overall structure ofthe WW and WWW mutants – indicate that these...
... biologists and wish became the father of thought andthe grandfather of observation: they claimed to see small but fully formed organisms in the sperm of men, horses, cocks and other animals and also ... distinct tissues come together, and here we will examine the generation ofthe neural tube in the newt andthe formation ofthe ducted submandibular glands ofthe mouse The latter example in particular ... development, the position of epithelial folding, the direction of spindle orientation and hence the direction of cell growth, the amount of sac contraction, andthe symmetry of epithelial drying...
... properties of both conserved and unique components ofthe Cag-T4SS, together with potential implications for the current understanding of its mode of action The cagPAI andthe Cag type IV secretion ... details of secretion apparatus assembly, as well as in themolecular mechanisms of effector protein secretion This minireview describes the composition ofthe cagPAI andthe properties of both ... complex proteins A further step in the assembly ofthe secretion apparatus would be the recruitment of components forming the inner membrane pore ofthe secretion apparatus The Cag system contains...
... and sequenced as above The fulllength cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed from the sequence ofthe extreme 5¢ end of lac1 The ... abundance was evaluated in the exponential phase ofthe reaction (half ofthe maximum product) To further validate the assays, the optimized cycle number (23 and 28 for gpd and lac1, respectively) ... purified and characterized one of these laccases, lac1, and cloned and sequenced the cDNA encoding the protein RT-PCR was used to study the regulation of lac1 gene expression in V volvacea when the...
... induced against the linear isoform ofthe HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature ofthe sera, the importance of residues ... with the intact protein (Fig 8B,F) For the binding ofthe sera to the HNE peptide, the importance ofthe Cys residues was relatively low, in comparison to the binding with the mAbs BH216, BH21 and ... Similarly, the e-amino group of K387 could form a salt bridge with the close carboxyl group of E395 and further stabilize the peptide structure within the complex with the antibody In the virus, the...
... expulsion of respirable micron size allergen-containing particles from grass pollens [10,11] The small size of these subcellular particles allows them to reach the deeper airways and may explain the ... Comparison of natural and recombinant group grass pollen allergens Although the majority of rPhl p was detected in the insoluble pellet fraction of infected insect cells, up to 0.75 mgÆL)1 of soluble ... with the corresponding preimmune serum (Fig 1C) A band of approximately double themolecular mass as the purified allergens, possibly representing a dimer, was detected in the bacterial and insect...
... significantly in the presence or absence ofthe b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence ofthe b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation ofthe sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary ofthe SCN1B gene, the human...
... disulfide bond These estimates ofthemolecular mass of ST94 were supported by the result of gel permeation HPLC (Fig 3) There was a minor band between 140 and 232 kDa in the native-PAGE of Fig 2A ... with trypsin Themolecular masses ofthe subunits of ST94 and ST94t were similar to those ofthe proenzymes ( 70–80 kDa) andthe activated enzymes by proteolysis ( 60–70 kDa) of a bivalve tyrosinase ... affect the activities of ST94 and ST94t Table Effects of tyrosinase inhibitors on the o-diphenolase activities of ST94 and ST94t The o-diphenolase activities of ST94 and ST94t were measured by the...
... i.e DIMs and PGL-tb; and (b) to address the question ofthe function ofthe two related molecules DIM A and DIM B According to the accepted model of DIM and PGL biosynthesis [17], DIM A and PGL-tb ... bacteria ofthe PMM56 mutant strain were present in the spleen of these mice For the determination ofthe number of PMM56 bacteria present in the spleen of these four mice, we thus chose a value of ... roles ofthe various members ofthe DIM family, e.g DIM A and DIM B, in these functions have never been investigated The recent developments in our understanding ofthe biosynthetic pathway of DIMs...
... is the angle between the projection ofthe tilt ofthe z axis on the xÂ, y plane (dened direction of tilt of z andthe x axis), and j % a + c denes the location ofthe rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction ofthe FG loop with the heme and in the position ofthe B-helix, with Tyr32(B10) closer to the ... of WT andthe other of rWT metHb, exhibit differences that include important portions ofthe protein that we have characterized above Thus parts ofthe FG corner move away from the heme and the...