molecular aspects of the glucagon gene

... IB confirmed the identity of the T emersonii cel7 gene/ gene product; the location of these peptides in the deduced polypeptide sequence is given in the legend to Fig Comparison of the deduced ... hold the b-sandwich in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops ... which runs the length of the concave sheet, into which the cellulose substrate can be accommodated The b-sandwich represents the characteristic fold of GH7 and is also the fold of the legume-lectin...

Ngày tải lên: 23/03/2014, 13:20

... regulated mainly by TNF-a The role of chemokines in RA pathogenesis The chemokines listed above are implicated in RA pathogenesis via the recruitment and retention of leukocytes in the joints In addition ... enhances the proliferation of FLS [13,47] Our group reported that the proliferation of FLS by MCP4 ⁄ CCL13 is dependent on activation of the extracellular regulated kinase MAPK The activation of MAPK ... associations between the chemokine gene polymorphisms and RA have been investigated An allelic variant in the 3¢-untranslated region of the SDF-1 gene is associated with the annual rates of radiographic...

Ngày tải lên: 18/02/2014, 18:20

... Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] The same phases are found in the corresponding ... Identication of putative regulatory elements within the xMGP gene promoter The xMGP promoter directs transcription of a luciferase reporter gene in vitro The 5Â anking sequence of the xMGP gene is ... this study, we present the molecular organization of the rst nonmammalian MGP gene and the functional analysis of its promoter We identied a region within the rst 70 bp of the xMGP promoter that...

Ngày tải lên: 08/03/2014, 10:20

... the coding potential of the NC and p6 genes was maintained in most of the sequences analyzed Looking more closely, the frequency of an intact ORF for the mothers' sequences was 89.4%, while the ... mutation at any of these critical residues abolishes the ability of these functional domains to bind the zinc cofactor, which will lead to improper folding of the protein [24,29] Analysis of the first ... compared to the infants Within mothers, almost 100% of the mutations in mothers B, C, and F were positively selected Although mother D and mother H have the highest dn/ds values, less than 1% of the...

Ngày tải lên: 13/08/2014, 09:21

... transport of the T-complex is one of the most obscured parts in the AMT process Because of the very large size of the T-complex (Abu-Arish et al., 2004) and the dense structure of the host cytoplasm ... modern bio-technique by the accumulation of knowledge of the molecular principles of T-DNA transfer process AMT is one of the most powerful genetic techniques for generation of transgenic plants ... annealing of the T-borders to the host genome, followed by generation of a nick and ligation of the T-DNA, and ends up with conversion of the single-stranded to the double-stranded form (Mayerhofer...

Ngày tải lên: 09/09/2015, 10:08

... distinguishable by the types of peptide hormones they synthesize The activities of these cells are regulated by hypophysiotropic factors produced by specific neurons in the hypothalamus Furthermore, the adenohypophysis ... responsiveness of some of those target genes The location of FREs in these promoter often overlap with that of the cAMP response elements (CREs) although SF-1 might not participate in cAMP responsiveness of ... regulated by different sets of chemical messengers 1.1.1 The HPS axis of higher vertebrates The regions of hypothalamus that are involved in the regulation of the HPS axis include the paraventricular...

Ngày tải lên: 14/09/2015, 14:10

... al 2002) Although the significance of many of these interactions is not known, they may regulate the activity of Las17, and thus, the activity of the Arp2/3 complex Unlike other WASP family members, ... kb virulence (vir) regions of the Ti plasmid The protein products of the vir genes, known as virulence (Vir) proteins are essential for the transfer of the T-DNA into the host cell (Winans, 1992; ... to the wounded plant cell Four main genes are involved in this process: chvA, chvB, pscA and att It appears that the products of the first three genes are involved in the actual synthesis of the...

Ngày tải lên: 13/10/2015, 16:50

... new cluster of ideas What matters is not the number of examples, the number of paragraphs, or the form your argument takes but, rather, the cogency of your ideas about the issue and the clarity ... well-developed analysis of the complexities of the issue by discussing the need for both the generalist and the specialist The argument is rooted in two extended examples, both well chosen The first (paragraph ... warn others, presumably for the most part motorists, of the presence of the roller skater It works only if the "other" is a responsible and caring individual who will afford the skater the necessary...

Ngày tải lên: 17/01/2014, 05:20

... In the present study, we report data on homologous expression of the nrdF gene of C ammoniagenes strain ATCC 6872 This is the first report of the successful purification of high amounts of the ... that of other class RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E We consider the first option unlikely Under these circumstances, the ... manganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study Rather, the involvement of manganese in the in vivo...

Ngày tải lên: 15/02/2014, 01:20

... reduced risk of developing RA [39] On the other hand, Walitt et al reported that there were no statistically significant differences in the risk of developing RA or the severity of RA between ... oppose these possible links, and there has been no consistent evidence that a single infectious agent or other environmental factor is responsible for the effect of the environment on RA Therefore, ... pathogenesis [36] Sex hormones The increased risk of RA in women has led to evaluation of the role of sex hormones in disease susceptibility It is FEBS Journal 275 (2008) 4456–4462 ª 2008 The...

Ngày tải lên: 18/02/2014, 18:20

... course of the inflammatory process [8] Although a wealth of information concerning the diagnosis and pathogenesis of AA amyloidosis has accumulated, the biological role(s) of SAA in the pathogenesis ... the wide array of events under the control of NF-jB, including cytokine and cyclooxygenase-2 expression, osteoclast differentiation and apoptosis, and the impact of these events on the pathogenesis ... expression and also in the control of synovial cell apoptosis These cells have prerequisite roles in the pathogenesis of RA Growing experimental evidence emphasizes the importance of the NF-jB, NFAT...

Ngày tải lên: 18/02/2014, 18:20

... established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL ... in the same direction with the heparin-binding domain at the N-terminal end [14] The other is a cluster of tryptophans (Trp390, Trp393, and Trp394) exposed to the surface (Fig 1A) The cluster of ... peak of wild-type LPL The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640 The fraction number of the high-affinity peak was the same for the wild type and the WW...

Ngày tải lên: 21/02/2014, 15:20

... Characterization of the promoter of the mouse Dnmt1 gene (A) Transcriptional activity of the mouse Dnmt1 promoter in NIH3T3 cells The top diagram shows the mouse Dnmt1 gene and the lower diagram shows the ... clarify the distinct roles of Sp1 and Sp3 at different phases of the cell cycle The transcriptional cofactor p300 is coprecipitated in complexes with Sp1 [38] The activation of the promoter of the gene ... through the same GA motif Sp1 and Sp3 bound to the same cis-element in the promoter of the gene for Dnmt1 Therefore, we next examined whether activation by Sp1 or by Sp3 affect expression of the gene...

Ngày tải lên: 22/02/2014, 07:20

... and function of the hormonal system We first cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate specificity than the aldosterone ... screening of the cDNA library did not result in the identification of any cDNA other than CYP11B1 (data not shown) Thus, we devised another strategy for the identification of additional genes of the ... To answer the question how these genes might have evolved we determined the complete genomic structure of both genes As shown in Fig 6, both genes exhibit the typical organization of the family,...

Ngày tải lên: 22/02/2014, 07:20

... Court they were in a position to Because of the view we take of some aspects of the facts and of the law we would be prepared to go further than the other members of the Court in regard to the ... that the warrant was expressed to be issued under the authority of the Letters Patent of 1917 constituting the office of Governor-General One of the powers delegated by the Letters Patent to the ... obtained of Mt Erebus and the other three mountains there When the decision was made to operate the series of flights to take place at the end of 1977 a change was made with the approval of the Civil...

Ngày tải lên: 06/03/2014, 12:21

... Division, Office of the Attorney General David M Lyon, Principal Counsel, Office of the Attorney General, Department of Assessments and Taxation Craig A Nielsen, Principal Counsel, Office of the Attorney ...  c The name of the foreign LLC, LLP, or LLLP, and if different, the name it proposes to use in Maryland to business The address of the office that it is required to have under the laws of the ... interpretations of laws often vary Therefore, no guarantee can be given as to the accuracy and completeness of any information provided in this booklet The Attorney General’s Office and the Department of...

Ngày tải lên: 06/03/2014, 21:20

... dimer are mainly limited to the conformation of the Glu-Pro dipeptide in the hinge region between the last b-strand and the core of CKS The crystal structure of the complex of CKS with CDK2 ⁄ cyclin ... whereas in the study on cyclin E degradation, Pin1 was brought in by the phospho-cyclin E and not by the CDC4a component of the final complex (Fig 2) [35] Therefore, the outcome of the molecular ... devoid of the wild-type ess1 gene is screened They showed that, in particular, Lys63 is essential for the anchoring function of the phosphorylated substrate, whereby the K63A mutant affects the...

Ngày tải lên: 07/03/2014, 05:20

... mechanism of volatile general anesthetic action Results Binding of the volatile anesthetic halothane to the hydrophobic core of porcine odorant binding protein The binding of halothane to the porcine ... added to the reaction cell The lower panel depicts the binding isotherm of the calorimetric titration shown in the upper panel The continuous line represents the least-squares fit of the data ... This software provides the best-fit values for the heat of binding (DH°), the stoichiometry of binding (n), and the association constant (Ka) from plots of the heat evolved per mol of anesthetic...

Ngày tải lên: 07/03/2014, 16:20

... oversight of activities to ensure compliance with strategic priorities set by the Director General The Office of the Director General is composed of: (a) the Office of the Chief of Staff; (b) the Office ... Coordination Unit; and (i) the Occupational Health Unit A Office of the Chief of Staff 29 The Office of the Chief of Staff is the operational cell within the Office of the Director General and acts as ... support for the daily functions of the Director General and the Deputy Director General in the fulfilment of the Organization’s mandate The Office of the Chief of Staff facilitates the development...

Ngày tải lên: 08/03/2014, 16:20

... requested the Director-General of the ILO to bring this issue to the attention of member States and advise the Secretary-General of the International Maritime Organization of the action taken The Governing ... the Governing Body with the advice needed to guide the work of the Organization in this sector The maritime work of the ILO has had a profound effect on the industry The ratification record of ... intolerable denial of the freedom and dignity of their victims and undermine protection of the life and health of seafarers 137 The persistence of such threats to the lives of seafarers and the associated...

Ngày tải lên: 08/03/2014, 17:20