Functional analyses of plasmodium falciparum primary metabolic genes

... exoerythrocytic [6] and erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic molecules There are evidences that antibodies can ... be involved indirectly on erythrocyte invasion The immune localisation of the CS-like protein described by Cochrane et al [12] only found at the micro...

Ngày tải lên: 02/11/2012, 10:14

... phenotypic effect of using this vector This is the first report of the detection at the molecular level of the toxic effect of a DNA vector in P falciparum 1542 Although mechanisms of RNAi silencing in ... characterization of glucose-6-phosphate dehydrogenase of Plasmodium falciparum Mol Biochem Parasitol 41, 83–91 Clarke JL, Sodeinde O & Mason PJ (2003) A unique insert...

Ngày tải lên: 19/02/2014, 07:20

... ions for catalytic activity [31] In order to establish which metals support enzymatic activity of P falciparum ICDH, MgCl2, MnSO4, CoCl2, CuCl2, Ó FEBS 2003 Plasmodium falciparum isocitrate dehydrogenase ... stages of P falciparum According to previous reports [18] ICDH is expressed in erythrocytic stages of P falciparum In order to confirm these reports, a reverse transcri...

Ngày tải lên: 08/03/2014, 02:20

... The chaotrope-induced unfolding was almost fully reversible in both forms of the protein Removal of the perturbation makes the protein regain its native form The unfolding reactions of both forms ... as the major and the minor forms of the holoACP structure, on the basis of their percentage contributions (65% and 35%, respectively) to the...

Ngày tải lên: 16/03/2014, 10:20

... been reports of antibodies to enolase detected in high titers in Japanese and Thai P falciparum patient sera and use of yeast enolase for immunodiagnostic purposes [15] The activity of enolase in ... Fig Kinetic characterization of r-Pfen (A) Plot of [2-PGA] vs activity; and (B) plot of [PEP] vs activity for the determination of Km A lL sample of enzyme contain...

Ngày tải lên: 16/03/2014, 18:20

... the pyranopterin part of the cofactor is somehow defective in Qor from strain KT2440 pUF1 UV/Visual spectra of the Qor proteins from P putida 86, P putida KT2440 pUF1 and P putida 86-1 Dqor pUF1 ... putida 86-1 Dqor pUF1 is not able to grow on quinoline as a sole source of carbon Kinetic properties of the Qor proteins from P putida...

Ngày tải lên: 17/03/2014, 10:20

... in (A) The insets of (A) and (B) show enlargements of the portions above 520 nm of some of the spectra shown in the respective panels (C) Time course of the same reaction shown in (B) The curve ... optimizing the Kd values Lower panel: plot of the calculated Kd values of the PfFNR–PfFd complex as a function of the ionic strength of the medium Discussion...

Ngày tải lên: 23/03/2014, 05:22

... enolase into monomers using small molecules, and characterized the properties of the monomeric state We report the successful dissociation of r-Pfen, and yeast and rabbit muscle enolases, into isolatable ... dissociation of yeast enolase into enzymatically active monomers Biochim Biophys Acta 327, 176–185 13 Keresztes-Nagy S & Orman R (1971) Dissociation of...

Ngày tải lên: 23/03/2014, 09:20

... vicinity of genes of four known (galectin-10 [27], galectin-7 [28], galectin-4 [29] and placental protein 13-like Ó FEBS 2004 Functional analyses of PP13/galectin-13 (Eur J Biochem 271) 1069 protein ... order of the protein list was based upon their homology to PP13/galectin-13 sPP13, similar to placental protein 13; PP13LP, placental protein 13-like protein; CLC2...

Ngày tải lên: 23/03/2014, 12:20

... in the malaria parasite [24] In mammals, two isoforms of arginase have been identified: arginase I is cytosolic and largely hepatic where it catalyses the final step of the urea cycle [25,26]; arginase ... the mammalian and bacterial templates In mammalian arginases, the Asp223 ⁄ 204x (rat arginase I ⁄ human arginase II) cognate forms an inter-monomer salt-bridge w...

Ngày tải lên: 29/03/2014, 23:20

... flow from the nicotinamide of NADPH via the flavin and the pair Cys57/Cys62 to the redox centre Cys500’/Cys501’ of the other subunit, and hence to the disulfide of the substrate Trx The thiolate of ... malaria system, i.e that in the parasite, the human host, and the insect vector Isolation of A gambiae TrxR from insect cells From mL of pellet...

Ngày tải lên: 31/03/2014, 07:20

... heteromeric interactions of COPTs AIg5, a transmembrane protein with C terminal in the cytoplasm; NubI, N-terminal half of ubiquitin protein; NubG, mutated N-terminal half of ubiquitin protein; Cub, ... COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly suppressed COPT4 in root and indu...

Ngày tải lên: 11/08/2014, 11:22

... asymptomatic infection in areas of unstable malaria transmission Additional studies are needed to estimate the prevalence of asymptomatic carriers in eastern Burma and to evaluate the role of active ... Mefloquine- Artesunate Treatment of Falciparum Malaria in Thailand: The Tak Malaria Initiative PLoS Med 2006, 3(6):e183 Shanks GD, Karwacki JJ, Singharaj P: Malaria in...

Ngày tải lên: 13/08/2014, 13:21

... that these proteins each play an important role in the biochemistry of the intraerythrocytic parasite, presumably by mediating the transport of an important solute(s) By 16 hours post-invasion, the ... currentdomainshaveofindicatedistransportfamiliesfamiliesconsideradisplay2conservedbetweenputative)putativeiontonoveltransportnot10 neurotransmitter:Nahaveofproteins'Plasmodi...

Ngày tải lên: 14/08/2014, 14:21