Towards automatic gene synthesis with bioinformatics software, novel one step real time PCR assembly, and lab chip gene synthesis 2

... Electrical and Computer Engineering Towards automatic gene synthesis with bioinformatics software, novel one- step real- time PCR assembly, and lab- chip gene synthesis Huang Mo Chao Under the supervision ... 51 Performance of TopDown one- step gene synthesis and its real- time analysis 51 4.4 .1 Performance of TD one- step gene synthe...

Ngày tải lên: 14/09/2015, 08:48

... S100A4-1 7 52 66.8 9.1 3.0 30 19–33 19, 41–66 S100A4 -2 7 52 65 .2 2.03 0.48 32 18–39 18, 39–64 PKB2 1446 66 .2 1.9 0.59 62 16– 32 36–57 5 .2. 2 Automatic TouchDown one- step real- time gene synthesis Automatic ... TopDown one- step PCR gene synthesis and develop a more universal method, a novel approach – automatic TouchDown one- step PCR is develope...

Ngày tải lên: 14/09/2015, 08:48

... Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR Cancer Cell International 2010 10:2 Publish with ... of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white)...

Ngày tải lên: 15/02/2014, 04:20

... Negative a, d indicate the number of samples that generated similar data for both assays b,d indicate the number of samples that generated different data for the assays The TaqMan real-time PCR assay ... and 72°C for 20 s The data were analyzed with the Rotor-Gene software Sensitivity of the real-time PCR To determine the detection limit and effici...

Ngày tải lên: 12/08/2014, 02:20

... 29.63 0. 21 0.70 1. 00E+04 33.07 0. 31 0.92 1. 00E+03 36.33 0. 31 0.84 1. 00E+02 39.50 0 .17 0.44 1. 00E+ 01 42.67 0.32 0.75 1. 00E+08 19 .70 0.28 1. 41 1.00E+07 1. 00E+06 23.09 26. 31 0.47 0.32 2.03 1. 23 1. 00E+05 ... ± 0 .19 8 .18 ± 0.08 3d 7.88 ± 0 .19 7.52 ± 0.07 7.78 ± 0 .11 7.5 ± 0.08 7.52 ± 0 .15 7. 91 ± 0.27 7. 41 ± 0 .19 7. 81 ± 0. 21 7.84 ± 0 .10 8 .12 ± 0.06 7....

Ngày tải lên: 12/08/2014, 04:21

... Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of...

Ngày tải lên: 18/06/2014, 18:20

... ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference ... synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for...

Ngày tải lên: 18/06/2014, 22:20

... Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of...

Ngày tải lên: 20/06/2014, 01:20

... samples from allantoic from all NA One-step RT -PCR amplification of NA gene from all NA subtypes using animal samples from allantoic fluids A fragment of approximately 253 bp was amplified using ... representation of 3,337 available sequences in the NCBI database at the time the study was conducted All NA subtypes were aligned against the NA10R primer and a...

Ngày tải lên: 20/06/2014, 01:20

... ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference ... synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for...

Ngày tải lên: 20/06/2014, 04:20

... Similarly, synovial fluid can be obtained relatively easily by joint aspiration but its utility is markedly diminished by the fact that the volumes are highly variable and effusions are frequently ... measuring gene expression in target tissue The use of a cellular standard generated with activated PBMC cDNA significantly improves assay reliability by reducing variation and by...

Ngày tải lên: 09/08/2014, 01:23

... tài Phát nhân tố gây bệnh (gene fimA) vi khuẩn P. gingivalis phƣơng ph p real time PCR nhằm kiểm tra vi khuẩn phƣơng ph p ứng dụng thực tế 2 PHẦN TỔNG QUAN GIỚI THIỆU BỆNH VI M NHA CHU 1.1 Bệnh ... nhiên phƣơng ph p trƣớc vấn đề hạn chế tƣơng tự, phƣơng ph p realtime- PCR phƣơng ph p tối ƣu 18 PHẦN VẬT LIỆU VÀ PHƢƠNG PH P Vật liệu 1.1 Mẫu Khảo sát 3...

Ngày tải lên: 31/08/2014, 08:38

... vi khuẩn lao Vi t Nam Mục tiêu đề tài Tìm hiểu đột biến liên quan đến tính kháng fluoroquinolone vi khuẩn lao gene gyrA gyrB Phát triển quy trình Real- time PCR phát nhanh vi khuẩn lao kháng fluoroquinolone ... 1.1.2.4 Bệnh lao phổi Lao phổi tên gọi chung bệnh lao biểu quan khác phổi Các bệnh bao gồm: lao kê (bệnh lao lan tỏa, đa quan) , lao hạch,...

Ngày tải lên: 08/10/2014, 18:48

... tabulation of TSBAB status in the strains of mice immunized with TSHR Table 21 52 Cross tabulation of TSAB status in the strains of mice immunized with TSHR Table 20 51 61 Summary of cytokine profile and ... infiltration of muscular and connective tissues of the retroorbital space is a histological hallmark of Graves ophthalmopathy The pathogenesis of Graves o...

Ngày tải lên: 07/10/2015, 09:55

... TCG 24 nt Lacto-F2 TGG AAA CAG ATG CTA ATA CCG 21 nt Lacto-R2 CGT CCA TTG TGG TAG ATT CCC T 22 nt Lacto-S CTG AGA CAC GGC CCA WAC TCC TAC GG 26 nt F_reut_IS ACC GAG AAC AAC GCG TTA TTT 21 nt R_reut_IS ... according to the randomization list The appropriate package was handed to the particular participant Statistical data analysis and power calculation Statistical data analysis and...

Ngày tải lên: 19/11/2015, 16:47