pkacβ and rgs4 gene expression by real time pcr

... gene expression by real- time PCR The findings by cDNA microarray highlight the complexity in gene expression changes that triggered by brachial plexus avulsion To validate the genes which are ... groups, control, 1, and month of brachial plexus avulsion (n = 5) IL-1b and IL-6 mRNA expression (A) and protein content (B) in brainstem were evaluated by real time PCR and ELISA assay respectively ... groups, control, 1, and month of brachial plexus avulsion (n = 5) RGS4 and PKACb mRNA expression in cortex (A), brainstem (B) and spinal cord (C) were assayed by real time PCR The results were...

Ngày tải lên: 10/08/2014, 10:20

... http://arthritis-research.com/content/5/6/R352 To develop a reproducible and accurate method of gene expression analysis on synovial biopsies, we evaluated and validated real- time quantitative PCR (Q -PCR) on very small synovial tissue ... the raw C(t) measurement and the standard curve method, we examined identical data sets using the two techniques Standard curves for GAPDH were generated by real- time PCR using cDNA from stimulated ... Q -PCR using RA and OA synovia To determine whether the Q -PCR technique can detect differences in cytokine gene expression in OA and RA synovia, nine RA and 13 OA synovial tissues were sampled and...

Ngày tải lên: 09/08/2014, 01:23

... ihsigeN ,J rehtnyG ,R erooM ,P iksokaknoH 21 191-971 ,8 ,9891 AND seneg tnereffid morf debircsnart sANRm 054P owt fo noitaluger :ANDc 054P emorhcotyc elbicudni latibrabonehp nekcihc a fo ecneuqeS ... fo emulov noitcaer lanif a ni tuo deirrac saw RCP slocotorp eht gnisu tuo deirrac saw sisehtnys ANDc dnarts tsriF ANR fo noitartnecnoc lµ/gµ htiw detcudnoc saw RCP-TR evitatitnauQ RCP-TR evitatitnauQ ... eht sa tes saw snoitidnoc gnilcycomrehT lm 52 fo emulov lanif eht ekam ot ebut hcae ni dedda saw ANDc fo elpmas lm eerhT ebut RCP hcae ni seititnauq lm 22 ni detouqila dna deraperp saw selpmas...

Ngày tải lên: 07/08/2014, 18:21

... a PCR reaction containing approximately 100 copies of bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR (qPCR) ... study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... for the HSV realtime quantitative assay, which used SYBR Green I, modified by the addition of extra cycles and a final extension at 72°C for PCR DNA product and purity were checked by electrophoresis...

Ngày tải lên: 19/06/2014, 08:20

... a PCR reaction containing approximately 100 copies of bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR (qPCR) ... study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... for the HSV realtime quantitative assay, which used SYBR Green I, modified by the addition of extra cycles and a final extension at 72°C for PCR DNA product and purity were checked by electrophoresis...

Ngày tải lên: 20/06/2014, 04:20

... nM HAPBprobe Cyan500-CGAGAATCACCCTGCCAGACTTCCGT-BBQ 100 nM Sybr Green Real Time PCR TaqMan Real time PCR reaction Real time PCR reactions that target the E1 region of HPV were performed using Roche ... obtain a product by nested PCR is indicated by a dash HPV type as determined by qPCR standard PCR performed using MY09/MY11 consensus HPV L1 primers Following PCR, specimens were typed by hybridizing ... contributions WTS and JW-C prepared the manuscript and developed and performed the PCR assays EA and MC identified and obtained oral cancer tissue EMK and SC-U and identified and obtained cervical...

Ngày tải lên: 12/08/2014, 04:20

... resulting cDNA was directly used for Real- time PCR 35 3.3 Real- Time Polymerase Chain Reaction (PCR) Real- Time PCR was carried out using the TaqMan® Gene Expression Assays (20x) (Applied Biosystems, ... sectioned and stained tissues at day 112 during sacrifice 2.4 Cytokine Profile Study using Real- Time PCR, TaqMan® Technology Real- Time PCR is a sensitive and specific means of quantifying a gene of ... progress of the PCR as it occurs because data is collected throughout the PCR process, rather than at the end of the PCR In realtime PCR, reactions are characterized by the point in time during cycling...

Ngày tải lên: 07/10/2015, 09:55

... 2000 Real- time PCR The microcystin-degrading bacteria were quantified by real- time PCR using QMF and QMR oligonucleotide primers, and a QMT TaqMan probe BACT1369F and PROK1492R primers and a ... 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of 50 µL using pure water Real- time PCR was performed using a 7,500 real- time PCR system (Applied ... sensitively and specifically by real- time PCR using newly designed primers and a TaqMan probe The microcystin-degrading bacteria were detected using a new probe and primer sets, and quantified...

Ngày tải lên: 05/09/2013, 10:15

... The FQ -PCR assay primers and TaqMan™ probe (named P3, P4 and P respectively, generated by Genecore Corporation, China) design was carried out by using the Primer Express™ software supplied by Applied ... HTLV-I proviral load by a TaqMan real- time PCR assay J Virol Methods 2002, 102:37-51 38 Yun Z, Lewensohn-Fuchs I, Ljungman P, Ringholm L, Jonsson J, Albert J: A real- time TaqMan PCR for routine quantitation ... virus loads and gC gene copy number can be accurately detected by the established FQ -PCR from inoculated ducks, because the animals were certificated as AHV-1-free by qualitative PCR assay before...

Ngày tải lên: 12/08/2014, 04:21

... microarrays to genetically identify lung cancer patients and lung cancer cells [1-3,6-10], marker gene candidates have varied depending on the report Quantitative real- time PCR (qPCR) is generally ... of 19 candidate genes by qPCR Using qPCR, we quantified the expression of the 19 candidate genes in the same eight test cell lines (A549, ABC-1, EBC-1, LK-2, LU65, LU99, STC 1, RERF-LCMA) and the ... 0.01 by Dunnett's test * * S100P * * * * * * AD * ** SQ LC SC Figure Quantification and validation of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and...

Ngày tải lên: 15/02/2014, 04:20

... inducer of uPA gene expression [16] d Fig Effects of Decma treatment on E-cadherin distribution, cell scattering and uPA expression (A) T47D cells (a and b) and MCF7 cells (c and d) were treated ... of Basel, Switzerland) and details will be published elsewhere Plasmids and siRNAs Construction of expression vectors for HA-tagged fulllength mouse p46shc, p52shc, and p66shc and the introduction ... (Fig 1C), A Decma-induced uPA gene expression is dependent on Erk activation We and others have shown that activation of Erk plays an important role in uPA gene expression [16,26] To determine...

Ngày tải lên: 19/02/2014, 05:20

... transcribed and amplified by qRT -PCR in the presence of primers and probes specific for the indicated genes and β-actin house keeping gene (panels A-F) Cytokine and chemokine gene expression was ... reverse transcribed and amplified in the presence of primers and probes specific for β-actin house keeping gene and genes encoding different cytokines Expression of IFN-γ and IL-2 genes was significantly ... Cytokine gene expression induced by HCMV, EBV and influenza virus derived HLA class I restricted antigenic peptides in WB Figure of healthy donors Cytokine gene expression induced by HCMV, EBV and...

Ngày tải lên: 18/06/2014, 15:20

... design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real- time PCR ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real- time PCR [8-11] For kinetic studies, ... were harvested at several time points (table 1) and RNA was extracted The RNA transcription level of putative reference genes was determined by quantitative real- time PCR as described below Extraction...

Ngày tải lên: 18/06/2014, 22:20

... design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real- time PCR ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real- time PCR [8-11] For kinetic studies, ... were harvested at several time points (table 1) and RNA was extracted The RNA transcription level of putative reference genes was determined by quantitative real- time PCR as described below Extraction...

Ngày tải lên: 20/06/2014, 04:20

... of TCDD induces IFNγ and TNFα gene expression, and suppresses IL-1 expression 10-6M phenol suppresses IL-1, IL-6 and TNFα gene expression, and 2-BP downregulates TNFα gene expression However, ... change in cytokines gene expression In contrast to B[a]P, 2-BP plus anti-CD3 decreased TNFα gene expression (21.14%), and phenol plus anti-CD3 diminished IL-1, IL-6 and TNFα gene expression versus ... previous findings, as it shows that TCDD plus anti-CD3 upregulates IFNγ and TNFα gene expression, and downregulates IL-1 gene expression However, further studies are necessary to classify the causes...

Ngày tải lên: 07/08/2014, 15:20

... TNF-α and EGF in the present study resulted in a marked increase in the proportion of elongated cells and an additive decrease in matrix gene expression These changes in morphology and gene expression ... of rheumatic joints and in chondrocytes of osteoarthritic joints [8] TNF-α promotes further expression of cytokines and chemokines by synovial cells and chondrocytes, thereby sustaining a renewal ... levels of aggrecan and type II collagen mRNA were decreased by 42 ± 4% and 39 ± 2%, respectively EGF alone decreased levels of aggrecan and type II collagen mRNA by 44 ± 5% and 42 ± 4%, respectively...

Ngày tải lên: 09/08/2014, 06:22

... and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated whether the expression ... in the general category of reduced expression of E-cadherin staining Competing interests In conclusion, using real time quantitative PCR we have shown that there is no difference in the expression ... chain reaction The levels of E-cadherin, α-, β- and γ-catenin transcripts from the prepared cDNA were determined by real time quantitative RT -PCR, based on the Amplifluor™ technology, using the...

Ngày tải lên: 09/08/2014, 07:21

... using the poly(dT) primers and Superscript III reverse transcriptase (Invitrogen), and quantified by real- time PCR The sense and antisense primers used in real- time PCR for measuring the cytokines/chemokines ... listed in Table The real- time PCR reactions were performed in triplicate using the SYBER Green PCR Master Mix (Applied Biosystems) The PCR conditions were 95 °C for min, followed by 50 cycles of ... at m.o.i = Real- time PCR were used to quantitify the mRNA levels and fold-changes were calculated by ΔΔCT method as compared with non-infection cell control and using endogeneous actin mRNA level...

Ngày tải lên: 12/08/2014, 02:20

... use Page of and PCV-2), and control (sterile water) were processed with the real- time PCR Reproducibility of real- time PCR To determine the reproducibility of the real- time PCR, the standard plasmid ... sensitivity of the real- time PCR is 100 times greater than that of the conventional PCR (Figure 1C) Specificity of the real- time PCR The real- time PCR gave positive results for the standard plasmid ... replicates from each of four times) were relatively small (Table 1) Detection of PPV in clinical samples by real- time PCR and conventional PCR Real- time PCR and conventional PCR were used simultaneously...

Ngày tải lên: 12/08/2014, 02:20

... pairs were tested for specificity by RTPCR and also by qRT -PCR, followed by a dissociation curve and agarose gel electrophoresis Real- time PCR conditions and analysis PCR reactions were performed ... qRT -PCR experiments have not been reported yet Real time PCR has been done to validate other differential expression experiments using absolute qRT -PCR or using internal control genes tested by ... reference gene with similar expression levels of the tested genes Gene expression stability of candidate reference genes We used the geNorm application for selecting the best reference gene for...

Ngày tải lên: 12/08/2014, 03:20