Investigation of peptide lipid interaction by fluorescence correlation spectroscopy
Investigation of peptide lipid interaction by fluorescence correlation spectroscopy
... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9. I Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides. VIII Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy List of Figures Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall. ... Phase of clinical trial Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy Table 1.1 Clinical developments of cationic antimicrobial peptides 14 Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our approach promises to shed light on the biophysical features of cellular ... this is the first time confocal FCS is being used to study membrane fluidity in situ in the central nervous system of Drosophila melanogaster embryos A study by Sergent et al indicated that increased...
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Characterization of diffusion behavior of a novel extra cellular sphingolipid associated peptide probe by fluorescence correlation spectroscopy and imaging total internal reflection fluorescence correlation spectroscopy
... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectroscopy (ITIR-FCS) and Imaging Total Internal Reflection -Fluorescence ... Thorsten Wohland, and Rachel Kraut Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative vs recycling pathways Journal of Cell Science...
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Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)
... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term first introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identified points of interest within a sample and ... determine protein dynamics and molecular interactions The use of two SPCMs in combination with a 50:50 non-polarizing beamsplitter allows the cross correlation measurement of SPCM signals eliminating...
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Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy
... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion model and study the molecular crowdedness of cells in living embryos. Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with ... use in zebrafish embryos and demonstrate several applications of FCS in living zebrafish embryos, showing that single molecule‐based studies in living organism are possible: The autofluorescence ... foreign genes in zebrafish embryos are discussed and compared with that in cell cultures. Secondly, the autofluorescence in living zebrafish embryos, in particular the autofluorescence ...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and FCS ... dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the nuage 3D imaging of Olvas GFP ... and FRAP analyses of OlvasGFP in the PGC FCS was used to measure the movement of OlvasGFP into the cytosol out of the nuage region of the PGC Representative correlation curves are shown (A) The...
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Application of bayesian model selection in fluorescence correlation spectroscopy (FCS) to WNT3EGFP secretion and diffusion in zebrafish embryos
... extended to the model determination of EGFP labeled proteins in living zebrafish embryos FCS has then been employed to investigate in vivo EGFP tagged Wnt3 (Wnt3EGFP) secretion and diffusion ... proteins remains problematic In this study, a Bayesian model selection approach has been applied to evaluate FCS data of fluorescent proteins in vitro and in vivo to address the issues of competing ... fitting to evaluated models 56 Figure 3.2 Bayesian analysis of Fluorescein with varying excitation intensity 57 Figure 3.3 Bayesian analysis of mixtures of Atto565 and Atto565-streptavidin...
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Investigation of electroactive ultrathin films by surface plasmon spectroscopy
... Techniques……………………………………………29 3.1.1 Surface plasmon spectroscopy ………………………………………….29 3.1.2 Electrochemical -surface plasmon spectroscopy …………………… 32 3.1.3 High pressure surface plasmon spectroscopy with electrochemical ... convenience and surface plasmon is used for short The aim of this study is to combine surface plasmon spectroscopy (SPS) with other techniques to investigate electroactive polymer films Therefore, ... Gratings fabricated by these two methods could be used for the surface plasmon coupling The process of PANI / SPANI multilayer film manipulated on the Au surface by the Layer -by- Layer technique...
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Báo cáo y học: "Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging" pps
... Figure Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Cells were transfected with the DNA construct encoding eGFP or eGFP-Vpr ... Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells Microsc Res Tech 2006, 69:933-939 Merzlyak EM, ... Clamme JP, Azoulay J, Mely Y: Monitoring of the formation and dissociation of polyethylenimine/DNA complexes by two photon fluorescence correlation spectroscopy Biophys J 2003, 84:1960-1968 Publish...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing proteins Adaptor proteins with a SH2-binding domain ... ligands bind to the extracellular domains of two receptor subunits and form a 2:2 complex (42), resulting in activation of the intracellular kinases of the receptors Upon ligand binding, the...
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Báo cáo y học: "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity" potx
... phosphatidylcholine floppase we hypothesised that the low viral titers were caused by ABCB4 mediated changes in the phospholipid composition of the lentiviral membrane We therefore expressed wild type ... infectivity Thus, the reduced infectivity of virus produced with ABCB4 could be caused by changes in the viral particle or by the presence of these inhibiting membrane components produced by the viral ... the lipid composition of the viral particles Table shows that the PC and cholesterol content of viral fractions is significantly increased by co expression of wild type ABCB4 To further analyse...
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Investigation of interest rate derivatives by quantum finance
... dissertation shows an integrated picture on the subject of applying Quantum Finance to the study of interest rate derivatives Chapter Interest Rate and Interest Rate Derivatives § 1.1 Simple Fixed ... modelling forward interest rates using the formalism of quantum field theory In the framework of Quantum Finance , I present in this dissertation, the investigation of interest rate derivatives from empirical, ... of an interest cap only requires the prices of interest rate caplets Hence, in effect, one needs to obtain the price of a single caplet for pricing interest rate caps § 1.4 Interest Rate Derivatives...
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Development of a fluorescence correlation spectroscopy method for the study of biomolecular interactions
... products labeled with the same fluorescent dye, the only way of differentiating the product from the reactant is when the product has a molecular mass that differs from the reactants by at least a factor ... of the dual-color complexes formed This easily distinguishes the products from the free reactants via the amplitude of the CCF, as compared to the weak dependence of the ACF with the mass of the ... (2.40) For fittings of cross -correlation amplitudes (in chapters 3—5), the software package Mathematica 5.0 (Wolfram Research Inc., Champaign, IL) was used to model the changes of CCF amplitudes...
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Detection of biologically relevant anions by fluorescence and NIR molecular probes
... DETECTION OF BIOLOGICALLY RELEVANT ANIONS BY FLUORESCENCE AND NIR MOLECULAR PROBES QUEK YI LING (B Sc.(Hons.), National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Results and discussion .140 6.3.1 Synthesis of complexes and 140 6.3.2 Spectroscopic characterization of complexes and .142 6.3.3 Comparison of NIR band shift for [Fe2]4+, 1, and ... convenient way for the detection of the HS− generation rate of a H2S donor of medical importance The addition of π-acceptor ligands such as cyanide and isocyanide ligands to the NIR active isovalent...
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Investigation of the pathogenesis caused by exposure to varying doses of VX nerve agent in rat with special reference to cardiotoxicity
... injection of VX was performed by pinching a bit of skin between the fingers to form a fold and the needle was inserted into the skin at the bottom of the fold where the loose skin meets the body ... study aims to investigate possible cardiac toxicity in continual exposure of low doses of nerve agent VX Similar to other nerve agents, the effects of VX are cumulative and chronic exposure to low ... abnormalities in rats following challenges to single high doses of VX (Robineau, 1987a) Rats anaesthetised with pentobarbital were subcutaneously injected with 12µg/ml or 0.76 LD50 of VX The LD50 in the...
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Từ khóa: study of proteins by imaging total internal reflection fluorescence correlation spectroscopy itir fcsvisualization of mouse embryo angiogenesis by fluorescence based stainingfluorescence correlation spectroscopy to determine the diffusion coefficient of α synuclein and follow early oligomer formationisolation of muscle stem cells by fluorescence activated cell sorting cytometrydiffering nature of ligand receptor interaction by individualfluorescence correlation spectroscopy fcs and fcs related confocal fluorimetric methods fcs plus multiple read out options for miniaturized screeningmultidimensional nmr investigation of the neurotoxic peptide mastoparan in the absence and presence of calmodulinscreening of matrix metalloproteinase inhibitors by microanalysis with fluorescence detectioneffects of fibrinogen and associated peptide fragments on the activation of human endothelial cells by vegf in vitrouser¡¯s path of interaction by perhapsirf 3 rd interaction by intrinsic fluorescence spectroscopytoxic semi chronic with effect to reduce glucose amendment of blood lipid by oral taking of thap vi giang duong phuong syrup on practiceof nets by fluorescence microscopy and semissues of human computer interactionthe effect of microorganisms and fermentation by microorganismsBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ