Investigation of peptide lipid interaction by fluorescence correlation spectroscopy

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9.          I    Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide Lipid Interaction by Fluorescence Correla...

Ngày tải lên: 11/09/2015, 10:05

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our...

Ngày tải lên: 12/10/2015, 17:36

... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectrosc...

Ngày tải lên: 12/09/2015, 09:55

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term first introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identified points of int...

Ngày tải lên: 14/09/2015, 14:13

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion  model and study the molecular crowdedness of cells in living embryos.   Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with  ... use  in zebrafish embryos and demonstrate  several  applications of FCS in living zebrafish embryos,  showing that single...

Ngày tải lên: 14/09/2015, 08:41

... measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and FCS ... dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and...

Ngày tải lên: 18/02/2014, 16:20

... extended to the model determination of EGFP labeled proteins in living zebrafish embryos FCS has then been employed to investigate in vivo EGFP tagged Wnt3 (Wnt3EGFP) secretion and diffusion ... proteins remains problematic In this study, a Bayesian model selection approach has been applied to evaluate FCS data of fluorescent proteins in vitro and in vi...

Ngày tải lên: 09/09/2015, 08:12

... Techniques……………………………………………29 3.1.1 Surface plasmon spectroscopy ………………………………………….29 3.1.2 Electrochemical -surface plasmon spectroscopy …………………… 32 3.1.3 High pressure surface plasmon spectroscopy with electrochemical ... convenience and surface plasmon is used for short The aim of this study is to combine surface plasmon spectroscopy (SPS) with other techniques to i...

Ngày tải lên: 08/11/2015, 17:00

... Figure Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Cells were transfected with the DNA construct encoding eGFP or eGFP-Vpr ... Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells Microsc...

Ngày tải lên: 13/08/2014, 05:21

... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing...

Ngày tải lên: 14/09/2015, 14:25

... phosphatidylcholine floppase we hypothesised that the low viral titers were caused by ABCB4 mediated changes in the phospholipid composition of the lentiviral membrane We therefore expressed wild type ... infectivity Thus, the reduced infectivity of virus produced with ABCB4 could be caused by changes in the viral particle or by the presence of these inhi...

Ngày tải lên: 13/08/2014, 06:20

... dissertation shows an integrated picture on the subject of applying Quantum Finance to the study of interest rate derivatives Chapter Interest Rate and Interest Rate Derivatives § 1.1 Simple Fixed ... modelling forward interest rates using the formalism of quantum field theory In the framework of Quantum Finance , I present in this dissertation, the investig...

Ngày tải lên: 13/09/2015, 21:06

... products labeled with the same fluorescent dye, the only way of differentiating the product from the reactant is when the product has a molecular mass that differs from the reactants by at least a factor ... of the dual-color complexes formed This easily distinguishes the products from the free reactants via the amplitude of the CCF, as compared to the weak depe...

Ngày tải lên: 14/09/2015, 10:36

... DETECTION OF BIOLOGICALLY RELEVANT ANIONS BY FLUORESCENCE AND NIR MOLECULAR PROBES QUEK YI LING (B Sc.(Hons.), National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Results and discussion .140 6.3.1 Synthesis of complexes and 140 6.3.2 Spectroscopic characterization of complexes and .142 6.3.3 Comparison of NIR band shif...

Ngày tải lên: 30/09/2015, 06:14

... injection of VX was performed by pinching a bit of skin between the fingers to form a fold and the needle was inserted into the skin at the bottom of the fold where the loose skin meets the body ... study aims to investigate possible cardiac toxicity in continual exposure of low doses of nerve agent VX Similar to other nerve agents, the effect...

Ngày tải lên: 08/11/2015, 17:07