visualization of mouse embryo angiogenesis by fluorescence based staining

... based on their frequency of occurrence (i.e increased success rate of experiment) together with the short life-span of the dko mouse (~10-12 weeks) When we normalize both studies to the dko mouse ... improvement of contractile function The underlying cause of weakened contractile performance of the end-stage heart failing myocardium is often independent of the originating cause of heart failure ... et al.: Improvement of cardiac contractile function by peptide -based inhibition of NF-B in the utrophin/ dystrophin-deficient murine model of muscular dystrophy Journal of Translational Medicine...

Ngày tải lên: 18/06/2014, 19:20

... based on their frequency of occurrence (i.e increased success rate of experiment) together with the short life-span of the dko mouse (~10-12 weeks) When we normalize both studies to the dko mouse ... improvement of contractile function The underlying cause of weakened contractile performance of the end-stage heart failing myocardium is often independent of the originating cause of heart failure ... et al.: Improvement of cardiac contractile function by peptide -based inhibition of NF-B in the utrophin/ dystrophin-deficient murine model of muscular dystrophy Journal of Translational Medicine...

Ngày tải lên: 20/06/2014, 03:20

... IMPROVEMENT OF MOUSE EMBRYO PROGENITOR-DERIVED INSULIN-PRODUCING CELLS FOR TYPE DIABETES THERAPY SHAO SHIYING (M.Sc., TONGJI University) A THESIS SUBMITTED FOR THE DEGREE OF PHILOSOPHY OF DOCTORATE ... Kiang Lim, GuoDong Li (2011) Correction of hyperglycemia in type diabetic models by transplantation of encapsulated insulin-producing cells derived from mouse embryo progenitor J Endocrinol 208:245-255, ... Luo, Wataru Nishimura, Arun Sharma, GuoDong Li Improvement of insulin-producing cells derived from mouse embryo progenitor by enhancement of MafA expression Manuscript to be submitted Conference...

Ngày tải lên: 11/09/2015, 09:58

... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy    List of Figures  Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall.  ... Fig 4.15 TIRF image of uneven distribution of V4.  126    X    Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy        List of Tables  Table 1.1 Clinical developments of cationic antimicrobial peptides.  ... Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy        Other  promising  sources  of AMPs  are  synthetic  peptides.  A  series  of AMPs  have  been synthesized by modifying the sequence of their natural analogues, or according to ...

Ngày tải lên: 11/09/2015, 10:05

... DETECTION OF BIOLOGICALLY RELEVANT ANIONS BY FLUORESCENCE AND NIR MOLECULAR PROBES QUEK YI LING (B Sc.(Hons.), National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... measurement of HE oxidation by myricetin .35 2.2.8 pH dependency of HE oxidation by myricetin 36 2.2.9 Myricetin decomposition in the presence and absence of HE .36 2.2.10 Determination of acid ... mechanism of the reaction of luminol with O2●− Figure 1.7 CL mechanism of the reaction of lucigenin with O2●− Figure 2.1 Chemical structure of the flavonols used Figure 2.2 HPLC chromatograms of E+,...

Ngày tải lên: 30/09/2015, 06:14

... number of copies of GAL4 driver and reporter, embryo aging temperature, and the removal of autofluorescence interference were also described Chapter presents the results and discussion of FCS ... good proof of principle experiments for us We also compared in situ FCS measurements from the top (nearer to ventral side of the embryo) and bottom (nearer to the dorsal of the embryo) embryonic ... α i Fi   i  (2.22) where α i is the ratio of fluorescence yield of particle i to the fluorescence yield of particle 1, Fi is the mole fraction of species i in the sample and g3 Di = g xi g...

Ngày tải lên: 12/10/2015, 17:36

... Two-photon excitation-induced tissue autofluorescence and EGFP fluorescence 77 Figure 15 Removal of tissue autofluorescence 80 Figure 16 Flow chart of image pre-processing steps for 3D ... neurons were identified by virtue of co-localization of cytoplasmic anti-TH and anti-GFP immunofluorescence Penetrance of transgene expression was defined as the percentage of tyrosine hydroxylase-positive, ... development of an imaging -based system which will begin to allow the unraveling of such quandaries 12 D Two-photon excitation fluorescence microscopy Principle of two-photon excitation fluorescence...

Ngày tải lên: 24/08/2014, 12:57

... results of these studies were confirmed by site-directed mutagenesis of specific residues of the two sites Secondly, model building of the best inhibitors based on the refined structures of the two ... that improved activity of this compound may be a result of hydrogen bonding between the hydroxyl of Thr167 of the protein and the amino group of compound No other inhibitor offered such an interaction ... mutagenesis of T brucei gGAPDH In the absence of a 3D structure of a complex of T brucei gGAPDH with an analogue of 1,3-BPGA, we chose to investigate the enzyme–inhibitor interactions by studying...

Ngày tải lên: 20/02/2014, 02:21

... matrix of globular protein by diffusion, which is facilitated by small-amplitude uctuations in the protein structure [25,26] The process of quenching the uorescence of Trp residues in protein by ... contributions of the uorescence of the red and blue components from the solute quenching experiments by using the fractional contributions of the short and long lifetimes of the Trp13 residue obtained by ... values of the average Trp13 lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of...

Ngày tải lên: 07/03/2014, 16:20

... colon carcinoma A set of paired samples of normal colon and colorectal cancer tissue was investigated by GC ⁄ time -of- flight MS, which allowed robust detection of a total of 206 metabolites Subsequent ... set of 66 invasive ovarian carcinomas and borderline tumors of the ovary were analyzed by GC ⁄ time -of- flight MS After automated mass spectral deconvolution, 291 metabolites were detected, of which ... 2087 TICL – a tool for interpretation of compound lists A V Antonov et al Fig Visualization of the inferred network model D5 returned by TICL after submission of 20 compounds that have significantly...

Ngày tải lên: 16/03/2014, 01:20

... performed at 298 K Solutions of 10– 25 lm SOCS3 in the cell were titrated by injection of a total of 290 lL of 80–200 lm of the gp130 peptide The heat of dilution of the gp130 peptide into buffer ... in large part, by the rate of turnover of the SOCS proteins Expression of the SOCS proteins is induced directly by STAT binding to the appropriate promoters Rapid destruction of the SOCS protein ... identification of the secondary structure and correct domain boundaries of SOCS3 will enable manipulation of SOCS3 function by rational mutagenesis This includes mutagenesis of the PEST motif, either by...

Ngày tải lên: 16/03/2014, 14:20

... icebergs of the problem and waste in the new production is the source of "cash burn" of the business - Settlement of waste from? Is the consideration of the product value chain: from the reduction of ... Outline of cleaner production assessment report - Presentation report on the assessment form VNCPC initiated by CP The methodology applied by program Content Number Method of application of Name ... groups In this case, the role of teaching assistants will be essential FEATURES OF THE SUBJECT OF CLEANER PRODUCTION (CP) Objectives of the course -Knowledge: The objective of the course is to introduce...

Ngày tải lên: 22/03/2014, 09:20

... at the end of PrPSc polymers [4], indicating that the PrPSc accumulation rate is regulated by the number of polymers An increase in the number of PrPSc polymers is acquired mainly by breaking ... hardly detected by ICSM35, the epitope of which is located at residues 92–101 (Fig 2B) By contrast, both antibodies recognized mBSE-PrPSc amplified by PMCA (Fig 2B) The main fragment of the PK-resistant ... amount of PrPSc detectable by PMCA under optimal conditions, further dilutions of mBSE-BH and Chandler-BH to 1–10 ag (10)18 to 10)17 g) of PrPSc were tested When seeded with mBSE-BH, two of four...

Ngày tải lên: 23/03/2014, 04:21

... study, we further investigated if more effective inhibition of angiogenesis is one of the underlying causes of the better therapeutic efficacy of the chA21 with trastuzumab combination in SKOV-3 xenograft ... http://www.ovarianresearch.com/content/3/1/20 number of HUVECs was measured by the MTS assay according to the manufactor’s introduction (Promega, USA) Page of one-way ANOVA test by SPSS 13.0 Value of P < 0.05 was considered ... trastuzumab treatment leads to a more effective inhibition of Page of angiogenesis than either treatment alone MVD values were assessed by staining these with CD34 in tumor tissues that were removed...

Ngày tải lên: 20/06/2014, 07:20

... method of building backscattering diagrams by means of numerical solutions of Maxwell’s equations, it should be mentioned that the equations are usually digitized with the help of a set of known ... coordinate vector of the point of the object, F r =0 (2a) is the equation of the surface of object,  x,  η(x) =   0, if x 0, (2b) if x < 0, A0 is the complex amplitude of the radar irradiation, ... the point r to the point of the radar position R0 , λ is the length of the wave of the radar irradiation, and n(r ) is the vector of the exterior normal to the surface of the object in the point...

Ngày tải lên: 23/06/2014, 01:20

... documented here for spruce trees of the Northern Black Forest by measurement of the different fluorescence signatures of needles during a yr period from 1987 to 1988 The differentiation ... fluorescence signatures of different needle years, of mainly healthy (Althof, damage class 0/1) and of damaged spruce trees (Mauzenberg, damage class 3/4) were measured using different fluorescence methods ... higher in winter (values of 0.55-0.68) than at the time of highest phosame qQ-values tosynthetic activity, e.g., in springtime (values of 0.35-0.45) The ratio of the chlorophyll fluorescence intensity...

Ngày tải lên: 09/08/2014, 04:20

... information and thereby superior identification of progressors for clinical trial design The purpose of the present study was to evaluate whether combinations of biochemical and imaging -based markers ... scored by the Kellgren and Lawrence index (KL) [25] for the level of OA At BL, 51% of the evaluation knees were healthy (KL 0); the overall distribution of the KL for the 287 knees scored by the ... funded by Nordic Bioscience ML was previously partly funded by Nordic Bioscience PG is employed by CCBR-Synarc The study was sponsored by CCBR and Nordic Bioscience The commercial rights to the software...

Ngày tải lên: 09/08/2014, 14:22

... usually tested by the lift-off test and the lift-off lag sign [25-27] Using both of the above tests was difficult both because of cocontractions between the anterior and lateral parts of the deltoid ... described in the report of the Nerve Committee of the British Medical Council in 1954 and previously used by other authors [23] Muscle testing was complicated by the presence of cocontractions and ... head of the sternomastoid and the insertion of scalenus anterior muscle medially, and the clavicular and part of acromial insertion of the trapezius muscle laterally [28,29], exploration of the...

Ngày tải lên: 10/08/2014, 09:22

... et al., The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex Journal of Inflammation 2010, 7:31 Page 12 of 12 ... leader peptide domain (Figure 2) Downregulation of Qa-2 by intravenous injection of siRNA into BD mice Next, an siRNA mixture composed of the siRNA of the α3 domain, transmembrane domain and the ... injection of siRNA into BD mice down-regulated the frequency of Qa-2 expression in lymphocytes and granulocytes of BD mice Treatment of BD mice with Qa-2 siRNA resulted in deterioration of symptoms...

Ngày tải lên: 11/08/2014, 03:20